NEW. Makingtheinvisible visible. heipha. Culture media designed with safety in mind

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1 NEW Makingtheinvisible visible heipha. Culture media designed with safety in mind

2 > Quality Assurance >> Certificate

3 Contents Contents Page Pharmaceutical and Cosmetic Products 6-18 The ICR Product Line 4-5 Triple wrapped, gamma-irradiated Culture Media for Hygiene Monitoring - ICR 6-9 Contact Plates - ICR 6 Contact Plates - ICR plus 6-7 Settle Plates 90 mm and 150 mm - ICR 8-9 Single wrapped, Culture Media for Hygiene Monitoring - RT and LI Contact Plates 10 Contact Plates - RT plus 11 Settle Plates 90 mm - LI Culture Media in Bags for Media Fills 12 Culture Media for Sterility Testing 13 Bottles 13 Culture Media for Mycoplasma Testing 14 Culture Media for Testing of Water for Pharmaceutical Use 15 Culture Media for Testing Non-Sterile Products according to EP + USP Plates 90 mm - LI 15 Plates 90 mm 16 Liquid Media in Bottles Liquid Media in Tubes 17 Agar Media in Bottles or Tubes 18 Slant Agar Tubes 18 1

4 Contents Contents Page Culture Media for Food, Beverages and Drinking Water Liquid Media for Sample Preparation, Pre-enrichment and Dilution 19 Media for Cultivation, Total Viable Count Selective Media Microbiological Investigation of Water and Drinking Water 32 Chromogenic Media 33 Dip Slides 34 Biochemical Reactions 34 Alphabetical index Service 42 Should the following pages not contain the media you need, please feel free to contact us regarding other media or filling volumes. 2

5 Culture Media Produced in Modern Facilities C u l t u r e M e d i a in Petri Dishes heipha is a leading supplier of culture media for medical microbiology and the pharmaceutical industry since The facility near Heidelberg, Germany, fully meets the quality standards of the pharmaceutical industry and is EN ISO certified. heipha operates in accordance with GMP-guidelines supported by a comprehensive quality management system. A p p l i c a t i o n : sterility testing product testing according to EP and USP air monitoring in commercially available sampling systems settle plates water testing personal hygiene monitoring Available as 90 mm and 150 mm plates. C u l t u re M e d i a in Contact Plates A p p l i c a t i o n : hygiene monitoring of surfaces air monitoring in commercially available sampling systems personnel hygiene monitoring The standard contact plates are 55 mm in diameter and have a 1 cm 2 grid on the base in order to facilitate counting. C u l t u r e M e d i a in Tubes, Bottles and Bags The product range includes a full line of culture media for hygiene monitoring and sterility testing according to USP and EP. For the use in sterile environments product safety is of the highest priority. This especially applies when ready-to-use culture media are used in isolators or clean rooms. Therefore heipha has specialized in the development and production of media for such high requirements. Culture media are available in tubes and bottles. Media in bottles are available with standard screw caps, wide mouth screw caps, septum stoppers and septum stoppers combined with screw caps. 3

6 The icr Product Line heipha consequently develops products to the special needs of the pharmaceutical industry. All ICR products are g-irradiated in the final packaging. Pharma / Cosmetics Petri dishes and contact plates are supplied in quantities of 10 and are triple bagged. This packaging variation ensures safe transfer into isolators and clean room areas. Additionally best protection against mechanical damage during handling is ensured. The inner bag is impermeable to H 2 O 2 and includes a pouch containing a drying agent to minimize the formation of condensation. The bag is provided with a hole at the sealed end for hanging up during the decontamination phase in isolators. All plates are stored at room temperature and individually labelled around the side of the plate with product name, expiry date, lot number and individual plate number. secure but easy to tear closure hole for hanging during gassing desiccant The triple packaging is designed for the special requirements in the most critical areas. outer bag middle bag inner bag 4

7 Culture Media - Hygiene Monitoring in Isolators and Clean Rooms Unique Features of heipha icr Plates: 1. Use in clean rooms and isolators = one product for all purposes = less validation 2. Storage at room temperature = no cooling capacity blocked = store at site of application 3. Extended shelf life = less lots to be tested (up to 9 months) = less documentation 4. Strong outer bag = maximum protection = easy to open 5. Plates without spacers = no desiccated plates = good recovery after long incubations ml filling volume = long monitoring times (settle plates) = better recovery rates ICR - Rely on Defined Conditions at Any Time! TSA Plates with LTHTh: why? Available as 2283e contact plate e settle plate, 30 ml a 150 mm plate, 80 ml Tryptic Soy Agar with LTHTh: inactivation of accumulated H 2O 2 during active air sampling - recovery of Staphylococcus epidermidis ATCC Pharma / Cosmetics 90% 91% 88% 89% During active air sampling low amounts of residual H 2O 2 are highly accumulated in the agar. Concentrations of even more than ppm H 2O 2 can be measured after actively sampling 1m 3 of air. Recovery rate Standard Tryptic Soy Agar (TSA) Tryptic Soy Agar with LTHTh (heipha code e) 0 ppm 0% ppm 0% 200 ppm H 2O 2 concentration per plate 1. more neutralizers = active against additional disinfectants 2. reducing agent = better growth of anaerobic microorganisms 3. H 2 O 2 -inactivation = better recovery rates in isolators 5

8 Culture Media - Hygiene Monitoring in Isolators and Clean Rooms ICR Culture Media - The Standard for Surface Monitoring storage at C gamma irradiated triple wrapped transparent H 2 O 2 -impermeable packaging long incubation time long shelf life Pharma / Cosmetics Contact Plates - 55 mm Tryptic Soy Contact Agar w. LT - ICR 2284e 9 20 (TSA w. lecithin, Tween 80) 200 Tryptic Soy Contact Agar w. LTHTh - ICR 2283e 9 20 (TSA w. lecithin, Tween 80, histidine, thiosulphate) 200 The new heipha standard for surface monitoring lock by turning clockwise long incubation at aerobic conditions lock by turning counterclockwise incubation at aerobic, microaerophilic or anaerobic conditions 6

9 Culture Media - Hygiene Monitoring in Isolators and Clean Rooms ICR plus - Culture Media - The New Standard for Surface Monitoring storage at C gamma irradiated triple wrapped transparent H 2 O 2 -impermeable packaging long incubations long shelf life plus: datamatrix-code on each plate (scanner in preparation) plus: lock by turning clockwise for long aerobic incubation plus: lock by turning counter clockwise for aerobic / anaerobic / microaerophilic incubation plus: safe transport of locked plates ICR plus Contact Plates - 55 mm NEW Blood Contact Agar + LTH - ICR plus (Blood Agar w. lecithin, Tween 80, histidine) 200 Pharma / Cosmetics NEW SDA Contact + LTHTh - ICR plus (SDA w. lecithin, Tween 80, histidine, thiosulphate, 200 replacement product for 25130e) NEW SDA Contact + LTHTh selective - ICR plus coloured - to avoid confusion in your clean room (see page 9) 200 (SDA w. lecithin, Tween 80, histidine, thiosulphate, irradiation-resistant antibiotics, replacement product for 25144e) NEW Tryptic Soy Contact Agar + LT - ICR plus (TSA w. lecithin, Tween 80) 200 NEW Tryptic Soy Contact Agar + LTHTh - ICR plus (TSA w. lecithin, Tween 80, histidine, thiosulphate) 200 NEW TSA Contact + LTHTh + ß-Lactamase II - ICR plus (TSA w. ß-lactamase II for inactivation of ß-lactam antibiotics, 200 lecithin, Tween 80, histidine, thiosulphate replacement product for 2281e) NEW TSA Contact + LTHTh + Penase - ICR plus (TSA w. penase for inactivation of penicillins + lecithin, 200 Tween 80, histidine, thiosulphate, replacement product for 2280e) 7

10 Culture Media - Hygiene Monitoring in Isolators and Clean Rooms ICR - Culture Media - The Standard for Active and Passive Air Sampling storage at C gamma irradiated, triple wrapped transparent H 2 O 2 -impermeable packaging long expositions / incubations long shelf life 30 ml filling volume Pharma / Cosmetics ICR Settle Plates - 90 mm NEW Blood Agar + LTH - ICR projected (lecithin, Tween 80, histidine) Sabouraud Dextrose Agar w. LTHTh - ICR 30 ml 03544e 6 20 (SDA w. lecithin, Tween 80, histidine, thiosulphate) 120 Sabouraud Dextrose Agar selective w. LTHTh - ICR 30 ml 03545e 6 20 coloured - to avoid confusion in your clean room (see page 9) 120 (SDA w. lecithin, Tween 80, histidine, thiosulphate, irradiation-resistant antibiotics) Tryptic Soy Agar - ICR 30 ml 03075e 9 20 (TSA) 120 Tryptic Soy Agar w. LT - ICR 30 ml e 9 20 (TSA w. lecithin, Tween 80) 120 Tryptic Soy Agar w. LTHTh - ICR 30 ml e 9 20 (TSA w. lecithin, Tween 80, histidine, thiosulphate) 120 Tryptic Soy Agar w. ß-Lactamase II - ICR 30 ml 03079e 6 20 (TSA w. ß-lactamase II for inactivation of ß-lactam antibiotics) 120 Tryptic Soy Agar w. LTHTh & Penase - ICR 30 ml 03086e 6 20 (TSA w. lecithin, Tween 80, histidine, thiosulphate + penase 120 for inactivation of penicillins) Settle plates after active air sampling of 0 liters of air and incubation of 7 days Conventional plate ICR plate 8

11 Culture Media - Hygiene Monitoring in Isolators and Clean Rooms ICR - Culture Media - The Standard for Active and Passive Air Sampling storage at C gamma irradiated, triple wrapped transparent H 2 O 2 -impermeable packaging long incubations 80 ml filling volume Settle Plate mm Tryptic Soy Agar w. LTHTh - ICR 15 cm a (TSA w. lecithin, Tween 80, histidine, thiosulphate; 3 plates each per triple bag) Pharma / Cosmetics Sabouraud TSA Sabouraud selective heipha ICR plates - experience the difference! 9

12 Single Wrapped Culture Media for Hygiene Monitoring of Surfaces RT and RT plus - Contact Plates RT storage at C long shelf life RT plus plus: datamatrix-code on each plate (scanner in preparation) plus: lock by turning clockwise for long aerobic incubation plus: lock by turning counter clockwise for aerobic / anaerobic / microaerophilic incubation plus: safe transport of locked plates Pharma / Cosmetics RT Contact Plates - 55 mm Contact Agar Baird Parker - RT 241e Contact Agar DG 18 + Chloramphenicol - RT 248e 6 20 (Dichloran Glycerol Agar) 200 Contact Agar MacConkey - RT 240e Contact Agar Malt Extract - RT 250e Contact Agar Plate Count - RT 238e Contact Agar Sabouraud Dextrose w. Chloramphenicol - RT 2511e Contact Agar Tryptic Soy - RT 235e Contact Agar Tryptic Soy w. LTH - RT 234e 9 20 (lecithin, Tween 80, histidine) 200 Contact Agar Violet Red Bile Dextrose (VRBD) - RT 245e Contact Agar Violet Red Bile Lactose (VRBL) - RT 243e RT plus Contact Plates NEW SDA Contact + Chloramphenicol - RT plus (intended to replace article 2511e) 200 NEW TSA Contact + LTH - RT plus (lecithin, Tween 80, histidine, intended to replace article 234e) 200 Standard and plus Contact Plates - (storage at 4-12 C) Contact Agar Columbia Blood 370e NEW Contact Agar Columbia Blood - plus (projected as replacement for article 370e) Single-wrapped media are not recommended for hygiene monitoring in clean rooms grade A or B

13 Single Wrapped Culture Media for Hygiene Monitoring LI - Settle Plates storage at C long expositions / incubations long shelf life 30 ml filling volume LI Settle Plates - 90 mm NEW Sabouraud Dextrose Agar (SDA) - LI acc. EP 03300e Sabouraud Dextrose Agar w. Chloramphenicol acc. EP - LI 03350e Pharma / Cosmetics Sabouraud Dextrose Agar w. LTHTh - LI 30 ml 03540e 6 20 (SDA w. lecithin, Tween 80, histidine, thiosulphate) 120 Tryptic Soy Agar (TSA) - LI 30 ml EP + USP 03074e Tryptic Soy Agar w. LTHTh - LI 30 ml e 6 20 (TSA w. lecithin, Tween 80, histidine, thiosulphate) 120 Tryptic Soy Agar w. LTH - LI 30 ml e 6 20 (TSA w. lecithin, Tween 80, histidine, cysteine) 120 Single-wrapped media are not recommended for hygiene monitoring in clean rooms grade A or B 11

14 Culture Media in Bags for Media Fills Tryptic Soy Broth in Bags Self collapsing, gamma-sterilised multi layer foil bags Highest safety by filling bags with autoclaved medium via sterile filtration Tryptic Soy Broth acc. to EP and USP 10 litre in 10 litre bag - gamma-irradiated l b 12 1 Pharma / Cosmetics or 200 litre in 200 litre bag 502bulk on request other volumes on request The 10 litre bags are packed into two further safety bags followed by gamma-irradiation at a dose of 9-20 kgy. The bags are supplied with an 80 cm outlet connection tube and a short outlet tube each containing a MPC-male connector for removal of the medium. In addition a separate septum is applied for supplementing the media. The 200 litre bag is supplied with two tubes with MPC-male and ACD connectors for removal of the medium. One outlet tube is designed as a dip tube through which the medium can be pumped of the container. The second outlet tube is positioned at the bottom side of the bag for removal of the medium by gravity. After filling the bags are packed into a transport container which is provided with an opening for the bottom tube outlet. 12

15 Culture Media - Sterility Testing Bottles with Different Caps Designed for a Variety of Handling Procedures Bottles Thioglycollate Medium Thioglycollate Medium (acc. to EP + USP) ml - septum, flip cap 510d Thioglycollate Medium (acc. to EP + USP) ml - septum, screw cap 510sd Thioglycollate Medium Clear ml - septum, flip cap 490d Pharma / Cosmetics Thioglycollate Medium Clear ml - septum, screw cap 490sd Tryptic Soy Broth Tryptic Soy Broth (acc. to EP + USP) ml - septum, flip cap 502d Tryptic Soy Broth (acc. to EP + USP) ml - septum, screw cap 502sd Wash Solution Fluid A ml - septum, flip cap 579d ml - septum, flip cap d 12 6 Fluid D ml - septum, flip cap 577d ml - septum, flip cap d 12 6 NaCl-Peptone-Buffer ph 7.0 (acc. to EP + USP) 300 ml - septum, flip cap d

16 Detection of Mycoplasmas in Biopharmaceuticals NEW MycoplasmaCultureMedia ready to use media formulations according to European Pharmacopoeia 6.0 (2.6.7.) suitable for testing according to 21 CFr (FrEY and HAYFLiCK Media) Description Reference shelflife units number inmonths perpack Pharma / Cosmetics Liquid Media in Bottles (Storage at < -15 C; thereof 3 months at 4-12 C after thawing) NEWFREYLiquidMediumacc.toEP (ml,detectionofm.synoviae) NEWFRIISLiquidMediumacc.toEP (ml,detectionofnon-avianmycoplasmas) NEWHAYFLICKLiquidMediumacc.toEP (ml,generaldetectionofmycoplasmas) Solid Media in 60 mm Plates (Storage at 4-12 C) NEWFREYAgaracc.toEP 097e (9ml,detectionofM.synoviae) NEWFRIISAgaracc.toEP 090e (9ml,detectionofnon-avianmycoplasmas) NEWHAYFLICKAgaracc.toEP 093e (9ml,generaldetectionofmycoplasmas) Notes: Availability on request flat charge for dry ice shipment of refrigerated broth media 14

17 Testing of Water and Non-Sterile Products LI Culture Media storage at C long shelf life single wrapped 30 ml filling volume * Media prepared according to harmonised chapters of EP, USP and JP Testing of Water for Pharmaceutical Use LI Plates - 90 mm R2A Agar - LI EP 30 ml 0178e R3A Agar - LI 30 ml 0173e 6 20 (for subculture of colonies from R2A Agar) 120 Pharma / Cosmetics Testing of non-sterile products according to EP + USP LI Plates - 90 mm Baird-Parker Agar - LI 0203e 6 20 (22 ml filling volume) 120 BPLS Agar - LI 30 ml 01042e 6 20 (Brillant-green Phenol-red Lactose Sucrose Agar) 120 *Cetrimide Agar - LI 30 ml 0110e * MacConkey Agar - LI 30 ml 0126e * Mannitol-Salt Agar - LI 30 ml 0128e Pseudomonas Agar F - LI 30 ml 01101e 9 20 (King s B Agar, detection of fluorescein) 120 Pseudomonas Agar P - LI 30 ml 01102e 9 20 (detection of pyocyanin) 120 NEW * Sabouraud Dextrose Agar (SDA) - LI 30 ml 03300e * Sabouraud Dextrose Agar w. Chloramphenicol - LI 30 ml 03350e * Tryptic Soy Agar (TSA) - LI 30 ml 03074e * Violet Red Bile Dextrose Agar (VRBD) - LI 30 ml 0185e * XLD Agar - LI 30 ml 0152e

18 Culture Media - Testing of Non-Sterile Products 90 mm Standard Plates storage at 4-12 C single wrapped 20 ml filling volume Plates - 90 mm Pharma / Cosmetics Potato Dextrose Agar 3700e 4 20 * Sabouraud Dextrose Agar (SDA) 3540e 4 20 * Sabouraud Dextrose Agar (SDA) w. Chloramphenicol 3350e 4 20 * Tryptic Soy Agar (TSA) 307e 4 20 Liquid Culture Media with Tamper-Proof Closures Bottles Lactose Broth (acc. to EP + USP) 90 ml in 275 ml wide neck W 12 6 ml in 125 ml screw cap MacConkey Broth (acc. to EP + USP) ml in 125 ml screw cap ml in 250 ml screw cap 526-B 12 6 ml in 275 ml wide neck 526-W 12 6 Mossel Broth (EE Broth) ml in 125 ml screw cap NEW * in 125 ml screw cap projected ml in 250 ml screw cap 528-B 8 6 ml in 275 ml wide neck 528-W ml in 0 ml screw cap * NaCl-Peptone Buffer ph 7.0 (acc. to EP + USP) 90 ml in 125 ml screw cap ml in 250 ml screw cap B 12 6 ml in 125 ml screw cap ml in 0 ml screw cap ml in 2 litre bag b * Media prepared according to harmonised chapters of EP, USP and JP 16

19 Culture Media - Testing of Non-Sterile Products Liquid Culture Media with Tamper-Proof Closures Bottles * NaCl-Peptone Buffer w. 0.1% Tween 80, ph 7.0 (acc. to EP + USP) 90 ml in 275 ml wide neck W ml in 250 ml screw cap ml in 0 ml screw cap NaCl-Peptone Buffer w. LTH (acc. to EP + USP) 90 ml in 275 ml wide neck W 12 6 NEW Sabouraud Dextrose Broth (acc. to EP + USP) ml in 125 ml screw cap Thioglycollate Medium (acc. to EP + USP) ml in 125 ml screw cap Tryptic Soy Broth (acc. to EP + USP) 90 ml in 275 ml wide neck W 12 6 ml in 125 ml screw cap ml in 200 ml screw cap 502-B 12 6 ml in 275 ml wide neck 502-W 12 6 Pharma / Cosmetics Further Media in 2 litres bags on request Media in bottles with septum or combined septum/screw cap, see page 13 Liquid Culture Media in Tubes Tubes Mossel Broth (EE Broth) 10 ml 2192r 8 20 NEW * Rappaport-Vassiliadis Broth acc. to EP 10 ml 3922r 8 20 Selenite-Cystine Broth (acc. to USP) 10 ml 2150r 8 20 Tetrathionate Broth 10 ml 2180r 6 20 * Thioglycollate Medium (acc. to EP + USP) 9 ml 2171r 6 20 * Tryptic Soy Broth (acc. to EP + USP) 9 ml 3080r * Tryptic Soy Broth w. LTHTh 10 ml 373r (lecithin, Tween 80, histidine, thiosulphate) * Media prepared according to harmonised chapters of EP, USP and JP 17

20 Culture Media - Testing of Non-Sterile Products Bottles and Tubes for Pour-Plate Method Bottles Pharma / Cosmetics * Sabouraud Dextrose Agar (acc. to EP + USP) 200 ml in 250 ml screw cap * Sabouraud Dextrose Agar w. Chloramphenicol (acc. to EP + USP) 200 ml in 250 ml screw cap * Tryptic Soy Agar (acc. to EP + USP) 200 ml in 250 ml screw cap Tubes - 18 ml * Sabouraud Dextrose Agar (acc. to EP + USP) 3351r * Sabouraud Dextrose Agar w. Chloramphenicol (EP + USP) 3350r Tryptic Soy Agar 3071r (TSA w. Dextrose) Culture Media in Slants * Sabouraud Dextrose Agar (acc. to EP + USP) 1380r * Tryptic Soy Agar (acc. to EP + USP) 307r * Media prepared according to harmonised chapters of EP, USP and JP 18

21 Microbiology of Food, Beverages and Drinking Water Sample Preparation / Pre-enrichment / Dilution Tubes Buffered Peptone Water 9 ml 193r NaCl physiological 10 ml 2080r NaCl physiological 5 ml 2081r Bottles Buffered Peptone Water NEW 225 ml in 250 ml screw cap bottle ml in 0 ml screw cap bottle NEW 2000 ml in 2 litre bag b Buffered Peptone Water w. 0.1% Tween 80 0 ml in 0 ml screw cap bottle Cultivation / Total Viable Count Columbia Blood Agar 109e DEV Nutrient Agar 3871e 4 20 Mod. Tween-Letheen Agar 361e 4 20 (lecithin, Tween 80, thiosulphate) Nutrient Agar 309e 4 20 (acc. ISO u. -2) Plate Count Agar 306e 4 20 (acc. to ISO 4833) Standard I Nutrient Agar 303e 4 20 TGE Agar 246e 4 20 (Tryptone Glucose Meat Extract Agar) Tryptic Soy Agar (TSA) 307e 4 20 Tryptic Soy Agar - LI 30 ml 03074e Tryptic Soy Agar w. LTHTh - LI 30 ml e 6 20 (TSA w. lecithin, Tween 80, histidine, thiosulphate; storage at C) 120 Food / Beverages 19

22 Microbiology of Food, Beverages and Drinking Water Cultivation / Total Viable Count 55 mm Contact Plates Contact Agar Columbia Blood 370e NEW Contact Agar Columbia Blood - plus (projected as replacement for article 370e) 200 Plate Count Contact Agar - RT 238e 9 20 (storage at C) 200 Contact Agar Tryptic Soy w. LTH - RT 234e 9 20 (TSA w. lecithin, Tween 80, histidine; storage at C) 200 Slant Tubes TSA, 6 ml 307r Agar in Tubes for Plate Count Method, 18 ml Food / Beverages DEV Nutrient Agar 3872r Orange Serum Agar 3780r (acidophilic micro-organisms) Plate Count Agar 3061r (acc. to ISO 4833) Tryptic Soy Agar w. Dextrose 3071r 12 Yeast Extract Agar 168r Agar in Bottles for Plate Count Method DEV Nutrient Agar 200 ml in 250 ml screw cap bottle Orange Serum Agar (acidophilic micro-organisms) 200 ml in 250 ml screw cap bottle Plate Count Agar (acc. to ISO 4833) 200 ml in 250 ml screw cap bottle ml in 500 ml screw cap bottle Plate Count Skim Milk Agar 200 ml in 250 ml screw cap bottle TSA (acc. to EP + USP) 200 ml in 250 ml screw cap bottle ml in 500 ml screw cap bottle

23 Microbiology of Food, Beverages and Drinking Water Cultivation / Total Viable Count Broth Media in Tubes TSB (Tryptic Soy Broth) 9 ml 308r TSB with LTHTh 10 ml 373r (lecithin, Tween 80, histidine, thiosulphate) Letheen Broth 9.9 ml 361r Broth Media in Bottles Tryptic Soy Broth (TSB) 90 ml in 275 ml wide mouth bottle W 12 6 ml in 275 ml wide mouth bottle 502-W 12 6 ml in 125 ml screw cap bottle ml in 250 ml screw cap bottle 502-B ml in 250 ml screw cap botlle ml in 0 ml screw cap bottle TSB with LT (lecithin, Tween 80) 90 ml in 125 ml screw cap bottle B 12 6 Mod. Tween Letheen Broth (lecithin, Tween 80, thiosulphate) 90 ml in 250 ml screw cap bottle B 6 6 Food / Beverages 21

24 Microbiology of Food, Beverages and Drinking Water Selective Media Anaerobic micro-organisms (Clostridia and sulfite reducing anaerobic bacteria) DRCM Agar 206e 4 20 Egg Yolk Lactose Agar 183e 4 20 (detection of Lecithinase, Lipase and Lactosedegradation) m-cp-agar 189e 4 20 (detection of C. perfringens in drinking water acc.to Europ. Council Directive 98/83/EC) Shahidi Ferguson Perfringens Agar (SFP) 2065e 4 20 Tryptose Sulfite Cycloserine Agar (TSC) 2066e 4 20 Bottles for Plate Count Method Food / Beverages DRCM Agar 200 ml in 250 ml screw cap bottle Tubes DRCM Agar, 10 ml 206r 6 20 Thioglycollate Broth w. vitamin K and haemin, 10 ml 217r 6 20 Bacillus Cereus Ident Agar 174e 3 20 (chromogenic medium) MYP Agar 192e 4 20 (Mannitol Egg Yolk Polymyxin Agar acc. to BAM 2001 of FDA) Bacillus cereus Agar (PEMBA) 182e 5 20 (Egg yolk Mannitol Bromothymol blue Agar) Bifidobacteria Bifido Agar 20580e

25 Microbiology of Food, Beverages and Drinking Water Selective Media Campylobacter Karmali Campylobacter Agar 3341e 4 20 Preston Campylobacter Agar mod. (mccda) 334e 4 20 (CCDA w. Amphotericin B acc. to ISO 10272) E. coli, Coliform bacteria and Enterobacteriaceae Coli 2G-Agar 111e 2 20 (Chromogenic medium) ECD Agar with MUG 1872e 4 20 (Fluorogenic medium) E. sakazakii-ident-agar acc. to ISO/DTS e 4 on request (chromogenic medium) Lactose TTC Agar w. Tergitol-7 166e 4 20 (Detection of coliform bacteria in drinking water acc.to Europ. Council Directive 98/83/EC) MacConkey Agar 126e 4 20 MacConkey Agar - LI 30 ml 0126e 6 20 (storage at C) 120 Nutrient Agar 309e 4 20 (acc. to ISO , -2) MacConkey Sorbitol Agar with MUG 125e 4 20 (fluorogenic medium) Violet Red Bile Dextrose Agar (VRBD) 185e 4 20 (acc. to ISO ) Violet Red Bile Dextrose Agar (VRBD) - LI 0185e 6 20 (acc. to ISO , storage at C) 120 Food / Beverages 23

26 Microbiology of Food, Beverages and Drinking Water Selective Media E. coli, Coliform bacteria and Enterobacteriaceae Violet Red Bile Lactose Agar (VRBL) 187e 4 20 VRB Agar with MUG 1871e 4 20 (Fluorogenic Medium) TBX Agar 1875e 4 20 (Trypton Bile Glucuronic Medium, chromogenic medium, acc. to ISO ) 55 mm Contact Plates (Storage C) MacConkey-Agar - RT 240e Violet Red Bile Dextrose Agar (VRBD) - RT 245e Violet Red Bile Lactose Agar (VRBL) - RT 243e Food / Beverages Slant Tubes / Tubes Glucose Agar, 10 ml 3900r 4 20 (tube for confirmation of Enterobacteriaceae acc. to ISO , -2) Kligler Agar Slant Tube, 8 ml 1222r Kligler Agar w. Urea Slant Tube, 8 ml 1220r Simmons Citrate Agar, Slant Tube 6 ml 140r SIM Agar, 8 ml 393r Agar in Bottles for Pour Plate Method Violet Red Bile Dextrose Agar (VRBD) (VRBD, acc. to ISO ) 200 ml in 250 ml bottle with screw cap Violet Red Bile Lactose Agar (VRBL) 200 ml in 250 ml bottle with screw cap Agar in Tubes for Pour Plate Method, 18 ml TBX Agar, 18 ml 1875r 4 20 (Trypton Bile Glucuronic Medium, chromogenic medium, acc. to ISO ) 24

27 Microbiology of Food, Beverages and Drinking Water Selective Media E. coli, Coliform bacteria and Enterobacteriaceae Broth Media in Tubes DEV Lactose Peptone Broth, 9 ml 388r (w. durham tube) Glucose Broth w. Bromthymol blue, 8 ml 397r (w. durham tube) Lauryl Sulfate Broth with MUG & Durham, 10 ml 188r (fluorogenic medium) Modified Lauryl Sulfate Tryptose Broth (mlst) 1888r on request w. Vancomycin acc. to ISO/DTS 22964, 10 ml Mossel Broth, 10 ml 2192r 8 20 (acc. to ISO ) Broth Media in Bottles Lactose Broth 90 ml in 275 ml bottle w. wide mouth W 12 6 ml in 125 ml bottle w. screw cap MacConkey Broth ml in 275 ml bottle w. wide mouth 526-W 12 6 ml in 125 ml bottle w. screw cap ml in 250 ml bottle w. screw cap 526-B 12 6 Mossel Broth (EE Broth acc. to ISO ) ml in 275 ml bottle w. wide mouth 528-W 8 6 ml in 125 ml bottle w. screw cap ml in 250 ml bottle w. screw cap 528-B ml in 0 ml bottle w. screw cap Food / Beverages 25

28 Microbiology of Food, Beverages and Drinking Water Selective Media Enterococci Bile Esculin Azide Agar 167e 4 20 (Detection of enterococci in drinking water acc. to Europ. Council Directive 98/83/EC) Citrate Azide Tween Carbonate Agar (CATC-Agar) 2190e 4 20 Enterococcus Agar 2191e 3 20 (detection of esculin hydrolsis) Slanetz Bartley Agar 2192e 4 20 (Detection of enterococci in drinking water acc.to Europ. Council Directive 98/83/EC) Broth Media in Tubes Brain Heart Infusion Broth, 9 ml 3110r 8 20 Food / Beverages Fungi, Yeasts and Moulds Candida Ident Agar 344e 3 20 (chromogenic medium) Dichloran Glycerol (DG18) Agar 199e 4 20 w. Chloramphenicol Kimmig Agar 136e 4 20 Malt Extract Agar 194e 4 20 Malt Extract Agar 1941e 4 20 w. Chloramphenicol Potato Dextrose Agar 3700e 4 20 Sabouraud Dextrose Agar (SDA) 3540e 4 20 Sabouraud Dextrose Agar - LI 30 ml 03300e 6 20 (storage at C) 120 Sabouraud Dextrose Agar (SDA) 3350e 4 20 w. Chloramphenicol 26

29 Microbiology of Food, Beverages and Drinking Water Selective Media Fungi, Yeasts and Moulds Sabouraud Dextrose Agar w. Chloramphenicol - LI 30 ml 03350e 6 20 (storage at C) 120 Wort Agar 356e 4 20 Yeast Glucose Chloramphenicol Agar (YGC) 3590e mm Contact Plates (Storage at C) Contact Agar DG e 6 20 (Dichloran Glycerol Agar, xerophilic moulds) 200 Contact Agar Malt Extract - RT 250e Sabouraud Dextrose (SDA) Contact Agar 2511e 9 20 w. Chloramphenicol - RT 200 Slant Tubes Sabouraud Dextrose Agar (SDA) 1380r Agar in Tubes for Plate Count Method Food / Beverages Malt Extract Agar, 23 ml 1940r Sabouraud Dextrose Agar (SDA), 18 ml 3351r Sabouraud Dextrose Agar (SDA) w. Chloramphenicol, 18 ml 3350r Agar in Bottles for Plate Count Method Sabouraud Dextrose Agar (SDA) 200 ml in 250 ml bottle w. screw cap Sabouraud Dextrose Agar (SDA) w. Chloramphenicol 200 ml in 250 ml bottle w. screw cap Wort Agar 200 ml in 250 ml bottle w. screw cap

30 Microbiology of Food, Beverages and Drinking Water Selective Media Fungi, Yeasts and Moulds Agar in Bottles for Plate Count Method Yeast Glucose Chloramphenicol Agar (YGC) 200 ml in 250 ml bottle w. screw cap Broth Medium in Bottles NEW Sabouraud Dextrose Broth (SDB) ml in 125 ml bottle w. screw cap Lactobacilli MRS Agar 184e 4 20 (acc. to De Man, Rogosa and Sharpe) Rogosa Agar mod. 2068e 3 20 Agar in Bottles for Plate Count Method Food / Beverages MRS Agar (acc. to De Man, Rogosa and Sharpe) 200 ml in 250 ml bottles w. screw cap Legionella Legionella GVPC Selective Agar 186e 3 20 Legionella BCYEa Agar 1860e 3 20 Legionella BMPAa Selective Agar 1861e 3 20 Legionella MWY Selective Agar 1862e 3 20 Listeria Listeria Agar acc. to Ottaviani a. Agosti 178e 3 20 (chromogenic medium acc. to ISO , -2) Oxford Listeria Selective Agar 196e 4 20 PALCAM Listeria Selective Agar 197e

31 Microbiology of Food, Beverages and Drinking Water Selective Media Listeria Broth Media Fraser Enrichment Broth (1/2) (acc. to ISO ) 225 ml in 250 ml bottle w. screw cap Fraser Enrichment Broth, 10 ml 196r 5 20 (acc. to ISO ) Listeria Enrichment Broth, 10 ml 197r 5 20 Pseudomonas Cetrimide Agar (Pseudomonas CN-Agar) 110e 4 20 (Detection of Pseudomonas aeruginosa in drinking water acc.to Europ. Council Directive 98/83/EC) Cephaloridine Fucidin Cetrimide Agar (CFC) 180e 4 20 GSP Agar (Pseudomonads / Aeromonads) 191e 4 20 (Glutamate Starch Phenol Red Agar acc. to Kielwein) Pseudomonas Agar F - LI 30 ml (PSFA) 01101e 9 20 (detection of fluorecein, storage at C) 120 Pseudomonas Agar P - LI 30 ml (PSPA) 01102e 9 20 (detection of pyocyanin, storage at C) 120 Slant Tubes Food / Beverages King s B Medium 1103r (Detection of Pseudomonas aeruginosa in drinking water acc.to Europ. Council Directive 98/83/EC) Broth Media in Bottles Malachite-green Medium 50 ml in 275 ml bottle w. wide mouth W 12 6 Salmonella / Shigella BPLS-Agar mod. 104e 4 20 (Brilliant-green Phenol-red Lactose Sucrose Agar, modified) Salmonella Elective Agar 143e 4 20 (Rambach Agar, chromogenic medium) 29

32 Microbiology of Food, Beverages and Drinking Water Selective Media Salmonella / Shigella Salmonella Ident Agar 149e 4 20 (Chromogenic medium) Salmonella-Shigella-Agar (SS-Agar) 142e 4 20 XLD Agar 152e 3 20 XLD Agar - LI 30 ml 0152e 4 20 (storage at C) 120 Media in Tubes Food / Beverages Urea Agar acc. to Christensen, 6 ml 398r MSRV Medium with Novobiocin, 12 ml 3526r 8 20 (semi-solid medium for detection of motile Salmonella) OF-Medium, 8 ml 390r 8 20 Rappaport Vassiliadis Medium (RVS), 10 ml 392r 8 20 (acc. to EN ISO 6579) 120 Selenite Cystine Broth, 10 ml 2150r 8 20 Tetrathionate Broth (MKTTn), 10 ml 2182r 6 20 (acc. to EN ISO 6579) Broth Medium in Bottles Selenite Cystine Broth ml in 275 ml bottle w. wide mouth 529-W 6 6 ml in 250 ml bottle w. screw cap 529-B 6 6 Staphylococci Baird Parker Agar 203e 4 20 (acc. to ISO ) Baird-Parker Agar - LI 0203e 4 20 (22 ml filling volume, acc. to ISO , storage at C)

33 Microbiology of Food, Beverages and Drinking Water Selective Media Staphylococci DNase Test Agar 2070e 4 20 (detection of DNase) Mannitol Salt Agar 128e 4 20 Mannitol Salt Agar - LI 30 ml 0128e 6 20 (storage at C) NEW RPF Agar projected (Rabbit Plasma Fibrinogen Agar acc. to ISO ) 55 mm Contact Plates (Storage at C) Contact Agar Baird Parker - RT 241e Broth Medium in Tubes Brain Heart Infusion Broth, 9 ml 3110r 8 20 Yersinia Food / Beverages Yersinia Agar (CIN) 395e 4 20 Broth Media in Tubes Phosphate Buffer Solution, 10 ml 2082r Yersinia Enrichment Broth (IC), 10 ml 395r

34 Microbiology of Food, Beverages and Drinking Water Media for drinking water (* according to EN Council Directive 98/83/EC) * Bile Esculin Azide Agar 167e 4 20 (Enterococci acc. to ISO ) * Cetrimide Agar (CN-Agar) 110e 4 20 (P aeruginosa acc. to pren ISO 12780) * Lactose TTC Agar w. Tergitol-7 166e 4 20 (E. coli acc. to ISO ) * m-cp-agar 189e 4 20 (Clostridium perfringens) * Slanetz-Bartley Agar 2192e 4 20 (Enterococci acc. to ISO ) Standard I Nutrient Agar 303e 4 20 TSA (Tryptone Soja Agar) 307e 4 20 (E. coli acc. to ISO ) Agar in Slant Tubes Food / Beverages * King s B Medium 1103r (P aeruginosa acc. to pren ISO 12780) Agar in Tubes for Pour Plate Method, 18 ml * Yeast Extract Agar 168r (Colony count acc. to EN ISO 6222) Broth Medium in Tubes * Tryptophan Broth, 8 ml 2220r (E. coli acc. to ISO ) 32

35 Chromogenic Media Bacillus spp. Cereus Ident Agar 174e 3 20 E. coli, coliform bacteria Coli 2G-Agar 111e 2 20 E. sakazakii Ident Agar acc. to ISO/DTS e 4 on request TBX Agar 1875e 4 20 (Tryptone Bile X-Glucuronid Agar, E. coli acc. to ISO or -3) Agar in Tubes TBX-Agar, 18 ml 1875r 4 20 (Tryptone Bile X-Glucuronid Agar, E. coli acc. to ISO ) Listeria Food / Beverages Listeria Agar acc. to Ottaviani a. Agosti 178e 3 20 (Listeria acc. to ISO a. -2) Salmonella Salmonella Elective Agar 143e 4 20 (Rambach-Agar) Salmonella Ident Agar 149e 4 20 Yeasts and Moulds Candida Ident Agar 344e

36 DIP Slides / Biochemical Reactions Dip Slides HYCON Dip Slide GK T / HS (T: semiquantitative total count of aerobic micro-organsims (TTC staining of colonies) HS: semiquantitative total count of yeasts and moulds) HYCON Dip Slide GK A / C (A: semiquantitative total count of aerobic micrfoorganisms C: semiquantitative total count of coliform bacteria Biochemical Reactions Food / Beverages Egg Yolk Lactose Agar 183e 4 20 (detection of Lecithinase, Lipase and Lactosedegradation) Glucose Broth w. Bromthymol blue, 8 ml 397r (w. durham tube) Urea Agar acc. to Christensen, 6 ml 398r Kligler Agar, Slant Tube, 8 ml 1222r Kligler Agar w. Urea, Slant Tube, 8 ml 1220r 6 20 Kovacs Indole Reagent, ml OF Medium, 8 ml 390r 8 20 SIM Agar, 8 ml 393r Simmons Citrate Agar, Slant Tube, 6 ml 140r Tryptophan Broth, 8 ml 2220r Gram Staining Gram Staining Set (approx. samples) (4 reagents, each 250 ml) 34

37 Alphabetical index Name Page(s) Anaerobica micro-organisms 22 Bacillus spp. 22 Bacillus cereus Agar (PEMBA) 22 Baird Parker Agar 30 Baird-Parker Agar - LI 22 ml 15, 30 Baird-Parker Contact Agar - RT 10, 31 Bifido Agar 22 Bile Esculin Azide Agar 26, 32 Biochemical Reactions 34 Blood Agar + LTH - ICR plus 8 Blood Contact Agar + LTH - ICR plus 7 BPLS Agar - LI 30 ml 15 BPLS-Agar mod. 29 Brain Heart Infusion Broth in tubes 26, 31 Brillant-green Phenol-red Lactose Sucrose Agar, see BPLS 15, 29 Buffered Peptone Water in tubes 19 Buffered Peptone Water in bottles and bags 19 Buffered Peptone Water w. 0.1% Tween 80 in bottles 19 Campylobacter spp. 22, 23 Candida Ident Agar 19 Cephaloridine Fucidin Cetrimide Agar (CFC) 29 Cereus Ident Agar 22, 33 Cetrimide Agar - LI 30 ml (according to harmonised pharmacopoeia) 19 Cetrimide Agar (Pseudomonas CN-Agar) 29, 32 Chapman-Agar, see Mannitol Salt Agar 15, 31 Chromogenic media 33 Citrate Azide Tween Carbonate Agar (CATC-Agar) 26 Clostridia 22 Coli 2G-Agar 23, 33 Coliform bacteria 15-18, Columbia Blood Agar 19 Columbia Blood Contact Agar 10, 20 Columbia Blood Contact Agar - plus 10, 20 Contact Agar Baird Parker - RT 10, 31 Contact Agar Columbia Blood 10, 20 Contact Agar Columbia Blood - plus 10, 20 Contact Agar DG 18 +Chloramphenicol - RT 10, 27 Contact Agar MacConkey - RT 10, 24 Contact Agar Malt Extract - RT 10, 27 Contact Agar Plate Count - RT 10, 20 Contact Agar Sabouraud Dextrose w. Chloramphenicol - RT 10, 27 Contact Agar Tryptic Soy - RT (+/- neutralizers) 10, 19 Contact Agar Violet Red Bile Dextrose (VRBD) - RT 10, 24 Contact Agar Violet Red Bile Lactose (VRBL) - RT 10, 24 Contact Plates - ICR and ICR plus (triple wrapped, gamma-irradiated)

38 Alphabetical index Name Page(s) Contact Plates - RT and RT plus (single wrapped) 10 Contact Plates - 55 mm standard and plus (single wrapped) 10 DEV Lactose Peptone Broth in tubes (w. durham tube) 25 DEV Nutrient Agar 19 DEV Nutrient Agar in tubes 20 DEV Nutrient Agar in bottles 20 DG18-Agar, see Dichloran Glycerol Agar 10, 26, 27 Dichloran Glycerol (DG18) Agar w. Chloramphenicol 26 Dichloran Glycerol (DG18) Agar w. Chloramphenicol - RT 10, 27 DNase Test Agar 31 DRCM Agar 22 DRCM Agar in tubes 22 DRCM Agar in bottles 22 Drinking water 32 E. sakazakii-ident-agar acc. to ISO/DTS , 33 ECD Agar with MUG 23 E. coli 15-18, EE Broth, see Mossel Broth 16, 17, 25 Egg Yolk Lactose Agar 22, 34 Egg Yolk Mannitol Bromthymol blue Agar, see PEMBA 22 Enterobacteriaceae 15-18, Enterococci 26 Enterococcus Agar 26 Fluid A in bottles 13 Fluid D in bottles 13 Fraser Enrichment Broth (1/2) (acc. to ISO ) in bottles 29 Fraser Enrichment Broth in tubes 29 FREY Agar acc. to EP / 60 mm plates 14 FREY Liquid Medium acc. to EP in bottles 14 FRIIS Agar acc. to EP / 60 mm plates 14 FRIIS Liquid Medium acc. to EP in bottles 14 Fungi 15-18, Glucose Agar in tubes (confirmation of E. coli) 24 Glucose Broth w. Bromthymol blue in tubes (w. durham tube) 25, 34 Glutamate Starch Phenol Red Agar acc. to Kielwein, see GSP Agar 29 Gram Staining Set (approx. samples) 34 GSP Agar (Pseudomonads / Aeromonads) 29 HAYFLICK Agar acc. to EP / 60 mm plates 14 HAYFLICK Liquid Medium acc. to EP in bottles 14 HYCON Dip Slide GK A / C 34 HYCON Dip Slide GK T / HS 34 Hygiene Monitoring

39 Alphabetical index Name Page(s) Karmali Campylobacter Agar 23 Kimmig Agar 26 King s B Medium 29, 32 Kligler Agar in slant tubes 24, 34 Kligler Agar w. Urea in slant tubes 24, 34 Kovacs Indole Reagent, ml 34 Lactobacilli 28 Lactose Broth in bottles 16, 25 Lactose TTC Agar w. Tergitol-7 33, 32 Lauryl Sulfate Broth with MUG & Durham in tubes 25 Legionella 28 Legionella BCYEa Agar 28 Legionella BMPAa Selective Agar 28 Legionella GVPC Selective Agar 28 Legionella MWY Selective Agar 28 Letheen Broth in bottles 21 Listeria Listeria Agar acc. to Ottaviani a. Agosti 28, 33 Listeria Enrichment Broth in tubes 29 MacConkey Agar / 90 mm standard plates 23 MacConkey Agar - LI 30 ml 15, 23 MacConkey Broth in bottles 16, 25 MacConkey Contact Agar - RT 10, 24 MacConkey Sorbitol Agar with MUG 23 Malachite-green Medium in bottles 29 Malt Extract Agar 26 Malt Extract Agar w. Chloramphenicol 26 Malt Extract Agar in tubes 27 Malt Extract Contact Agar - RT 10, 27 Mannitol Salt Agar 31 Mannitol Salt Agar - LI 30 ml (according to harmonised pharmacopoeia) 15, 31 Mannitol Egg yolk Bromothymol blue Agar (PEMBA), see Bacillus cereus Agar 22 Mannitol Egg yolk Polymyxin Agar, see MYP Agar 22 mccda, see Preston Campylobacter Agar mod. 23 m-cp-agar 22, 32 Media Fills 12 Mod. Tween Letheen Broth in bottles 21 Mod. Tween-Letheen Agar 19 Modified Lauryl Sulfate Tryptose Broth (mlst) w. Vancomycin acc. to ISO/DTS in tubes 25 Mossel Broth (EE Broth acc. to ISO ) in tubes 17, 25 Mossel Broth (EE Broth acc. to ISO ) in bottles 16, 25 Mossel Broth in bottles (according to harmonised pharmacopoeia) 16 Moulds 15-18, MRS Agar 28 MRS Agar (acc. to De Man, Rogosa and Sharpe) in bottles 28 37

40 Alphabetical index Name Page(s) MSRV Medium with Novobiocin in tubes 30 Mycoplasmas 14 MYP Agar 22 NaCl physiological in tubes 19 NaCl-Peptone Buffer ph 7.0 (acc. to EP) in bottles 13, 16 NaCl-Peptone Buffer w. 0.1% Tween 80, ph 7.0 (acc. to EP) in bottles 17 NaCl-Peptone Buffer w. LTH in bottles 17 Non-sterile pharmaceutical products Nutrient Agar 19, 23, 32 OF Medium in tubes 30, 34 Orange Serum Agar in tubes 20 Orange Serum Agar, in bottles 20 Oxford Listeria Selective Agar 28 PALCAM Listeria Selective Agar 28 PEMBA 22 Phosphate Buffer Solution in tubes 20 Plate Count Agar 19 Plate Count Agar in bottles 20 Plate Count Contact Agar - RT 10, 20 Plate Count Skim Milk Agar in bottles 20 Potato Dextrose Agar 16, 26 Preston Campylobacter Agar mod., (mccda) 23 Pseudomonas 15-18, 29 Pseudomonas Agar P - LI 30 ml (PSPA) 15, 29 Pseudomonas Agar F - LI 30 ml (PSFA) 15, 29 Rabbit Plasma Fibrinogen Agar, see RPF Agar 31 Rambach Agar, see Salmonella Elective Agar 29, 33 R2A Agar - LI 30 ml 15 R3A Agar - LI 30 ml 15 Rappaport Vassiliadis Medium (RVS) in tubes 17 Rappaport-Vassiliadis Broth in tubes (according to harmonised pharmacopoeia) 30 Rogosa Agar mod. 28 RPF Agar 31 Sabouraud Dextrose Agar (+/- Chloramphenicol) / 90 mm plate 16, 26 Sabouraud Dextrose Agar (+/- antibiotics, + neutralizers) / 90 mm ICR plates 30 ml 8 Sabouraud Dextrose Agar (+/- Chloramphenicol, +/- neutralizers) / 90 mm LI plates 30 ml 11, 15 Sabouraud Dextrose Agar in slant tubes 27 Sabouraud Dextrose Agar in tubes (+/- Chloramphenicol) 27 Sabouraud Dextrose Agar in bottles (+/- Chloramphenicol) 27 Sabouraud Dextrose Contact Agar (+/- antibiotics, + neutralizers) / 55 mm ICR plus plates 7 Sabouraud Dextrose Contact Agar (+/- Chloramphenicol) / 55 mm RT and RT plus plates 10, 27 Sabouraud Dextrose Broth in bottles 28 38

41 Alphabetical index Name Page(s) Salmonella 15-18, Salmonella Elective Agar 29, 33 Salmonella Ident Agar 30, 33 Salmonella-Shigella-Agar (SS-Agar) 30 Settle plates ICR, 90 mm and 150 mm (triple wrapped, gamma-irradiated) 8, 9 Settle plates LI, 90 mm (single wrapped) 11 SDA, see Sabouraud Dextrose Agar SDB, see Sabouraud-Dextrose Broth 28 Selenite Cystine Broth in tubes 17, 30 Selenite Cystine Broth in bottles 30 SFP Agar, see Shahidi Ferguson Perfringens Agar 22 Shahidi Ferguson Perfringens Agar (SFP) 22 SIM Agar in tubes 24, 34 Simmons Citrate Agar in slant tubes 24, 34 Slanetz Bartley Agar 26, 32 Soybean Casein Digest Agar or Broth, see TSA or TSB Standard I Nutrient Agar 19, 32 Staphylococci 15-18, Sterility testing 13 TBX Agar 24, 33 TBX Agar in tubes 24, 33 Tetrathionate Broth (MKTTn) in tubes 30 Tetrathionate Broth in tubes 17 TGE Agar 19 Thioglycollate Broth w. vitamin K and haemin, 10 ml 22 Thioglycollate Medium in tubes 17 Thioglycollate Medium in bottles 13, 17 Thioglycollate Medium Clear in bottles 13 Tryptic Soy Agar (+/- neutralizers) / 90 mm plates 16, 19, 32 Tryptic Soy Agar (+/-neutralizers) / 90 mm and 150 mm ICR plates 30 ml or 80 ml 8, 9 Tryptic Soy Agar with Penase and neutralizers / 90 mm ICR plates 30 ml 8 Tryptic Soy Agar with ß-Lactamase II / 90 mm ICR plates 30 ml 8 Tryptic Soy Agar (+/- neutralizers) / 90 mm LI plates 30 ml 11, 15, 19 Tryptic Soy Agar in tubes 18, 20 Tryptic Soy Agar in slant tubes 18, 20 Tryptic Soy Agar in bottles 18, 20 Tryptic Soy Contact Agar (with neutralizers) / 55 mm ICR- and ICR plus plates 6, 7 Tryptic Soy Contact Agar with Penase and neutralizers / 55 mm ICR plus plates 7 Tryptic Soy Contact Agar with ß-Lactamase II and neutralizers / 55 mm ICR plus plates 7 Tryptic Soy Contact Agar (+/- neutralizers) / 55 mm RT plates 10, 19 Tryptic Soy Broth (+/- neutralizers) in tubes 17, 21 Tryptic Soy Broth (+/- neutralizers) in bottles 13, 17, 21 Tryptic Soy Broth in bags (10 litre and 200 litre) 12 Tryptone Glucose Meat Extract Agar, see TGE Agar 19 Tryptophan Broth in tubes 32, 34 Tryptose Sulfite Cycloserine Agar (TSC) 22 39

42 Alphabetical index Name Page(s) TSA, see Tryptic Soy Agar TSB, see Tryptic Soy Broth TSC, see Tryptose Sulfite Cycloserine Agar 22 Tween Letheen Broth in bottles, modified 21 Tween-Letheen Agar, modified 12 Urea Agar acc. to Christensen in tubes 30, 34 Violet Red Bile Dextrose Agar (VRBD) 23 Violet Red Bile Dextrose Agar (VRBD) - LI 30 ml 15 Violet Red Bile Dextrose Agar (VRBD) in bottles 24 Violet Red Bile Dextrose (VRBD) Contact Agar - RT 10, 24 Violet Red Bile Lactose Agar (VRBL) 24 Violet Red Bile Lactose Agar (VRBL) in bottles 24 Violet Red Bile Lactose (VRBL) Contact Agar - RT 10, 24 VRB Agar with MUG 24 VRBL, see Violet Red Bile Lactose Agar 10, 24 VRBD, see Violet Red Bile Dextrose Agar 10, 15, 23, 24 Water. Pharmaceutical Use / Drinking Water 15, 32 Wort Agar 27 Wort Agar in bottles 27 XLD Agar 30 XLD Agar - LI 30 ml 15, 30 Yeasts 15-18, Yeast Extract Agar in tubes 20, 32 Yeast Glucose Chloramphenicol Agar (YGC) 27 Yeast Glucose Chloramphenicol Agar (YGC) in bottles 28 Yersinia spp. 31 Yersinia Agar (CIN) 31 Yersinia Enrichment Broth (IC), 10 ml 31 40

43 Notes

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