WATER Microbiology BIOMARK LABORATORIES. Drinking Water. 1. Campylobacter Species. Dehydrated Culture Media. 1. Total Plate Counts

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1 Drinking Water 1. Total Plate Counts B869 M-(HPC) Heterotrophic Plate Count Agar Base B1213 M-(HPC) Heterotrophic Plate Count Broth Base B654 Nutrient Agar, ph 6.8 B298 Plate Count Agar (Standard Method Agar) B299 B399 B767 B790 Plate Count Agar R-2A Agar TGB Agar (Tryptone Glucose Beef Extract Agar) Tryptone Glucose Extract Agar (Tryptone Glucose Yeast Extract Agar) Selective Culture Media for Detecting Organisms in Water and Waste Water I. Isolation and Cultivation 1. Campylobacter Species B116 B945 BF018 B125 BF016 BF021 B951 BF016 B126 BF016 B1159 B964 BF013 BF015 B966 BF033 B994 Blood Agar Base No.2 Blood Free Campylobacter Selectivity Agar Base Campylobacter Supplement V (BFCSA) Brucella Agar Base Campylobacter Growth Supplement Horse Serum Brucella Agar Base, Modified Campylobacter Growth Supplement Brucella Broth Base Campylobacter Growth Supplement Campylo Thioglycollate Medium Base Campylobacter Agar Base Campylobacter Supplement - I (Blaser - Wang) Campylobacter Supplement -II (Skirrow) Campylobacter Enrichment Broth Base (Preston Enrichment Broth Base) Campylobacter Selective Supplement IV (Preston Selective Supplement) Columbia Blood Agar Base BF013 BF014 BF015 BF016 BF041 BF042 B657 BF033 B994 BF013 BF014 BF015 BF016 BF041 BF042 B657 BF033 Campylobacter Supplement - I(Blaser-Wang) Campylobacter Supplement - Ii (Butzler) Campylobacter Supplement - III (skirrow) Campylobacter Growth Supplement Campylobacter Selective Supplement Campylobacter Supplement VI (Butzler) Preston Agar Base Campylobacter Selective Supplement IV (Preston Selective Supplement) Columbia Blood Agar Base Campylobacter Supplement - I(Blaser-Wang) Campylobacter Supplement - Ii (Butzler) Campylobacter Supplement - III (skirrow) Campylobacter Growth Supplement Campylobacter Selective Supplement Campylobacter Supplement VI (Butzler) Preston Agar Base Campylobacter Selective Supplement IV (Preston Selective Supplement) 40

2 II. Biochemical Characterization B286 Phenol Red Dextrose Broth B309 SIM Medium B048 Triple Sugar Iron Agar 2. Clostridium perfringens B499 B887 B1211 Differential Reinforced Clostridial Broth Base Liver Meat Infusion Agar M-CP Agar Base BF119 BF120 M-CP Selective Supplement - I M-CP Selective Supplement - II 1. MPN Presumptive Test 2. Clostridium perfringens A. Total Coliforms (Faecal and Non-faecal) B024 B316 Fluid Lactose Medium Lactose Broth B991 B245 Lauryl Sulphate Broth (Lauryl Tryptose Broth) MacConkey Broth Purple w/ BCP II. MPN Confirmed Test B015 Brilliant Green Bile Broth 2% B180 EMB Agar, Levine B514 Endo Agar B1024 Endo Agar, Modified B830 MUG MacConkey Agar B603 MacConkey Agar w/1.35% Agar, CV, NaCl & 0.15% Bile Salts B1226 Mineral Modified Glutamate Medium Base (Double Strength) (Twin Pack) B515 Endo Agar Base BF052 Basic Fuchsin B771 Tergitol - 7 Agar Base B351 Violet Red Bile Agar B. Faecal Coliforms B900 B1139 B177 B387 A-1 Broth B.T.B. Lactose Agar, Modified (Lactose Blue Agar) EC Broth Lauryl Tryptose Mannitol Broth w/tryptophan B860 B235 B B602 B602 I M 7 Hr FC Agar MUG Brilliant Green Bile Broth MUG Bromocresol Purple Broth w/lactose MUG EC Broth MUG EC Broth, Modified 41

3 B. Faecal Coliforms B837 B1221 B830 MUG Lauryl Sulphate Broth MUG Nutrient Agar MUG MacConkey Agar B495 B753 PA Broth Sorbitol Agar (MacConkey Sorbitol Agar) I. Membrane Filter Technique B878 B876 B875 B874 B870 M-EC Test Agar M-Endo Agar LES M-Endo Broth M-Endo Broth MF (MF Endo Medium) (M-Coliform Broth) M-FC Agar Base, Modified B1100 B1195 B1214 B1215 B868 B868 I B573 M-FC Broth Base M-Filter Rinse Broth M-HD Endo Broth M-HD Broth w/bg M-Lauryl Sulphate Broth M-Lauryl Sulphate Broth M-Tergitol 7 Agar Base C. Differentiation of Faecal and Nonfaecal Coliforms B441 B475 B484 B164 B543 B215 B1198 Bromo Cresol Purple Broth Base Crystal Violet Lactose Broth Decarboxylase Test Medium Base Deoxycholate Lactose Agar (Hynes) Glucose Phosphate Broth Koser Citrate Medium Lactose Peptone Broth B763 B245 B161 B579 B319 B1127 MacConkey Broth (Double Strength) w/neutral Red MacConkey Broth Purple w/bcp Moeller Decarboxylase Broth Base Motility Test Medium Simmons Citrate Agar Tryptone Broth (Tryptone Water) D. Coliphage Detection B039 Soyabean Casein Digest Agar B040 Soyabean Casein Digest Medium I. MPN Test 4. Faecal Streptococci B107 B539 B698 I BF121 Azide Dextrose Broth Glucose Azide Broth MUD SF Broth Base Enterococcus Selective Supplement B640 Pfizer Selective Enterococcus Agar B707 SF Broth B341 Salt Broth, Modified 42

4 II. Membrane Filter Technique 4. Faecal Streptococci B422 Bile Esculin Agar B119 Brain Heart Infusion Agar B121 Brain Heart Infusion Broth B1077 KF Streptococcal Agar Base BF067 Bromo Cresol Purple B571 Kanamycin Esculin Azide Agar B222 M-Azide Broth Base B406 M-Enterococcus Agar Base B872 M-Enterococcus Agar Base, Modified III. Plate Count B425 Bile Escuin Azide Agar B1077 KF Streptococcal Agar Base B211 KF Streptococcal Broth Base IV. Presumptive Confirmed Test B107 B425 B949 B186 B187 B1028 Azide Dextrose Broth Bile Escuin Azide Agar Bromo Cresol Purple Azide Broth Enterococcus Confirmatory Agar Enterococcus Confirmatory Broth Enterococcus Presumptive Broth B518 B520 B503 B296 B320 Esculin Iron Agar Ethyl Violet Azide Broth (E.V.A. Broth) Ethyl Violet Azide Dextrose Agar Nutrient Gelatin Slanetz & Bartley Medium 5. Klebsiella Species I. Selective Isolation B243 MacConkey Agar, Modified II. Identification B215 B543 Koser Citrate Medium MR-VP Medium (Glucose Phosphate Broth) B319 B1127 Simmons Citrate Agar Tryptone Broth (Tryptone Water) III. Membrane Filter Technique B870 M-FC Agar Base, Modified BF078 Rosolic Acid 43

5 6. Legionella Species B127 BF025 BF026 B1034 B1035 B1202 BF131 Buffered Charcoal Yeast Extract Agar Base Legionella Selective Supplement IV (MWY) Legionella Supplement Feeley Gorman Agar (F.G. Agar) Feeley Gorman Broth (F.G.Broth) Legionella Agar Base Legionella Growth Supplement BF132 BF133 B627 BF023 BF024 BF025 BF026 Legionella Selective Supplement Legionella Supplement Modified Buffered Charcoal Agar Base Legionella Selective Supplement II Legionella Selective Supplement III Legionella Selective Supplement IV (MWY) Legionella Supplement 7. Leptospira species B1039 Fletcher Leptospira Medium Base (Leptospira Medium Base, Fletcher) BF021 B586 Horse serum Leptospira Medium Base, Korthof, Modified 8. Salmonella and Shigellae A. Qualitative Test I. Pre - Enrichment B024 Fluid Lactose Medium B040 Soyabean Casein Digest Medium II. Selective Enrichment B197 B244 B763 BW763 B245 B760 GN Broth, Hajna MacConkey Broth w/neutral Red MacConkey Broth (Double Strength) w/neutral Red MacConkey Broth (Double Strength) w/neutral Red MacConkey Broth Purple w/bcp MacConkey Vroth Purple B264 B386 B317 B026 B027 B332 Mueller Kauffman Tetrathionate Broth Base Rappaport Vassiliadis Medium Selenite Broth (Selenite F Broth) Selenite Cystine Broth Tetrathionate Broth Base (w/o Iodine & BG) Tetrathionate Broth Base, Hajna (TT Broth Base) 44

6 III. Selective Isolation B114 B.G. Sulpha Agar (Brilliant Green Sulpha Agar) B115 Bismuth Sulphite Agar B429 Bismuth Sulphite Agar, Modified B123 Brilliant Green Agar Base, Modified BF020 Sulpha Supplement B124 Brilliant Green Agar Base w/ 1.2% Agar BF020 Sulpha Supplement B016 Brilliant Green Bile Agar B015 Brilliant Green Bile Broth 2% B163 Deoxycholate Citrate Agar B022 EMB Agar B180 EMB Agar, Levine B023 EMB Broth B203 Hektoen Enteric Agar B1210 Leifson Agar B1126 Leifson's Deoxycholate Agar, Modified B236 MacConkey Agar w/o CV, NaCl, w/0.15% sodium Taurocholate B237 MacConkey Agar w/o CV, NaCl w/0.5% Bile Salts B238 B779 B603 B240 B241 B242 B604 B274 B310 B365 B345 B360 B361 MacConkey Agar w/0.15% Bile Salts, CV & NaCl MacConkey Agar w/cv, NaCl & 0.15% Bile Salts MacConkey Agar w/1.35% Agar, CV, NaCl & 0.15% Bile Salts MacConkey Agar w/o CV, w/0.15% Bile Salts MacConkey Agarw/o CV, w/1.2% Agar MacConkey Agar w/o CV,w/0.5% Bile Salts MacConkey Agar w/bromo Thymol Blue Nutrient Broth S S Agar Salmonella Differential Agar (Twin Pack) (RajHans Medium) Salmonella Differential Agar, Modified (Twin Pack) Xylose Lysine Agar Base Xylose Lysine Deoxycholate Agar (XLD Agar) IV. Identification & Biochemical Characterization B212 B215 B232 B596 BF077 B880 B247 B634 B590 B261 B286 B675 Kligler Iron Agar Koser Citrate Agar Lysine Iron Agar Lysine Iron Cystine Broth Base Novobiocin Selective Supplement Lysine Lactose Broth Malonate Broth, Ewing, Modified Moeller Decarboxylase Broth w/lysine HCl Motility Indole Lysine Medium (MIL Medium) Motility Sulphide Medium Phenol Red Dextrose Broth Phenol Red Dulcitol Broth B289 Phenol Red Lactose Broth B293 Phenol Red Mannitol Broth B295 Phenol Red Sucrose Broth B683 Phenylalanine Agar B684 Phenylalanine Malonate Broth (shaw & Clarke Medium) B309 SIM Medium B319 Simmons Citrate Agar B048 Triple Sugar Iron Agar BW1128 Tryptophan Medium B347 Urea Agar Base (Christensen) ( Autoclavable) BF048 Urea 40% B496 Urea Broth Base BF048 Urea 40% 45

7 B. Quantitative Tests I. MPN Presumptive B317 B026 Selenite Broth Selenite Cystine Broth B027 Tetrathionate Broth Base (w/o Iodine & BG) II. MPN Confirmed B123 BF020 B124 BF020 B232 Brilliant Green Agar Base, Modified Sulpha Supplement Brilliant Green Agar Base w/1.2% Agar Sulpha Supplement Lysine Iron Agar B596 BF077 B048 B361 Lysine Iron Cystine Broth Base Novobiocin Selective Supplement Triple Sugar Iron Agar Xylose Lysine Deoxycholate Agar (XLD Agar) III. Membrane Filter Technique B669 B850 M-Bismuth Sulphite Broth M-Brilliant Green Broth B862 M-Tetrathionate Broth Base 9. Vibrio Cholera & NAG Vibrios I. Enrichment B398 Alkaline Peptone Water II. Selective Isolation B042 TCBS Agar B372 VP Medium B811 B387 Vibrio Agar Vibrio Parahaemolyticus Sucrose Agar III. Identification B633 Moeller Decarboxylase Broth w/arginine HCl B634 Moeller Decarboxylase Broth w/lysine HCl B635 Moeller Decarboxylase Broth w/ornithine HCl B295 Phenol Red Sucrose Broth B797 Tryptone Soya Agar w/added NaCl B347 Urea Agar Base (Christensen) (Autoclavable) BF048 Urea 40% BW356 Wagatsuma Agar Base I. Enrichment 10. Yersinia Enterocolitica B662 PSB Broth Base B663 PSTA Enrichment Broth Base 46

8 II. Selective Isolation B002 I B001 I Yersinia Isolation Agar Yersinia Selective Agar Base BF102 Yersinia Selective Supplement III. Identification B424 BF011 B444 B445 B232 B633 B634 B635 Bile Esculin Agar Base Esculin CAL Agar (Cellobiose Arginine Lysine Agar) CAL Broth (Cellobiose Arginine Lysine Broth) Lysine Iron Agar Moeller Decarboxylase Broth w/arginine HCl Moeller Decarboxylase Broth w/lysine HCl Moeller Decarboxylase Broth w/ornithine HCl B579 B286 B675 B289 B293 B295 B683 B319 Motility Test Medium Phenol Red Dextrose Broth Phenol Red Dulcitol Broth Phenol Red Lactose Broth Phenol Red Mannitol Broth Phenol Red Sucrose Broth Phenylalanine Agar Simmons Citrate Agar Recreational Water Selective Culture Media for Detection of Organisms in Recreational Water 1. Actinomyces Species B393 B395 B1051 Actinomyces Agar Actinomycete Isolation Agar Garrod Actinomyces Medium B654 Nutrient Agar, ph 6.8 B746 Starch Casein Agar 2. Aeromonas species B918 BF001 B920 BF001 B234 Aeromonas Isolation Medium Base Aeromonas Selective Supplement Ampicillin Dextrin Agar Base Ampicillin Dextrin Selective Supplement M-Aeromonas Selective Agar Base(Havelaar) B388 BF083 B702 BF094 RS Medium Base Novobiocin Supplement Rippey-Cabelli Agar Base Rippey Cabelli Selective Supplement 47

9 3. Iron & Sulphur Bacteria B456 B958 B568 B752 Casitone Glycerol Yeast Autolysate Broth Base (CGY) Iron Oxidizing Medium (Twin Pack) Isolation Medium For Iron Bacteria Maintenance (SCY) Medium B697 B761 B762 B137 MN Agar Base Sulphate Reducing Medium (Twin Pack) Sulphate Reducing Medium (Triple Pack) Sulphur Medium (Twin Pack) 4. Pseudomonas aeruginosa B911 B842 Acetamide broth (Twin Pack) Aspargine Broth (Coccidioidin & Histoplasmin Broth) Aspargine Proline Broth Cetrimide Agar Base Nalidixic Selective Supplement Cetrimide Broth Cetrimide Broth Base B933 B975 BF035 B139 B976 B1014 Drake's Medium 10 B866 M-PA Agar Base B714 B706 I B302 B303 B695 BF092 BF093 B414 B304 Milk Agar (Brown & Scott Modified) (Twin Pack) Milk Agar w/cetrimide (Twin Pack) Pseudomonas Agar (For Fluorescein) Pseudomonas Agar (For Pyocyanin) Pseudomonas Agar Base Cetrinix Supplement CFC Supplement Pseudomonas Aspargine Broth Pseudomonas isolation Agar Base 5. Staphylococci B106 Azide Blood Agar Base B013 Baird Parker Agar Base BF007 Egg Yolk Tellurite Emulsion BF003 Egg Yolk Emulsion BF008 Potassium Tellurite 3.5% BF009 B P Sulpha Supplement B475 Crystal Violet Lactose Agar BF007 Egg Yolk Tellurite Emulsion B896 Lipovitellin Salt Mannitol Agar Base BF003 Egg Yolk Emulsion B863 M-Staphylococcus Broth B053 Mannitol Salt Agar Base BF003 Egg Yolk Emulsion B665 Phenylethyl Alcohol Agar B534 Staphylococcus Agar No Streptococcus mitis-salivarius B258 Mitis Salivarius Agar Base BF010 Potassium Tellurite 1% 48

10 7. Fungi - Yeasts & Molds B473 B156 B157 B480 B1008 B279 Corn Meal Agar Czapek Dox Agar Czapek Dox Broth Czapek Malt Agar Diamalt Agar Oxytetra Glucose Yeast Agar Base (OGYE Agar Base) BF086 B307 BF004 B703 B557 B363 Oxytetra Selective Supplement Rose Bengal Agar Base Chloramphenicol Selective Supplement Rose Bengal Chloramphenicol Agar Yeast Malt Agar (YM Agar) Yeast Malt Broth (YM Broth) 49