EQUIVALENCE TABLE BETWEEN ISO RECOMMENDED CULTURE MEDIA AND BIOLIFE CULTURE MEDIA MICROBIOLOGICAL METHODS FOR FOOD AND FEEDING STUFFS.

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1 EQUIVALENCE TABLE BETWEEN ISO RECOMMENDED CULTURE MEDIA AND BIOLIFE CULTURE MEDIA MICROBIOLOGICAL METHODS FOR FOOD AND FEEDING STUFFS. rev 6-06/2016 APPLICATION SAMPLE PREPARATION PEPTONE SALT SOLUTION MAXIMUM RECOVERY DILUENT ISO :1999 BUFFERED PEPTONE WATER* BUFFERED PEPTONE WATER ISO :2003 DIPOTASSIUM HYDROGEN PHOSPHATE DILUENT ISO :2003 SODIUM CITRATE DILUENT ISO :2003 PEPTONE SOLUTION TRYPTONE ISO :2010 QUARTER-STRENGTH RINGER'S SOLUTION ISO :2013 Details: ISO 7218:2007 Peptone Salt Solution Milk Peptone Salt Solution Liquid milk products Sodium citrate or di-potassium hydrogen phosphate ph 7.5 Cheese & processed cheese Peptone Salt Solution (room temperature) Frozen milk products including edible ices Peptone Salt Solution(pre-warmed to about 45 C) Butter Di-potassium hydrogen phosphate ph 7.5 Fermented milk, yoghurt, sour cream Di-potassium hydrogen phosphate ph 7.5 Dried milk Peptone Salt Solution Dried sweet whey, dried buttermilk, lactose Di-potassium hydrogen phosphate ph 8.4 Acid casein, lactic casein acid whey powder Di-potassium hydrogen phosphate ph 7.5 Caseinate Peptone Salt Solution (pre-warmed to 40 C) Chocolate and Chocolate confectionary *: may be used instead of PSS for preparation of the homogenate when a single 10-1 homogenate is made for both detection of Salmonella and enumeration of other organisms ENUMERATION OF MICROORGANISMS AT 30 C PLATE COUNT AGAR (PCA) TRYPTIC GLUCOSE YEAST AGAR :2013 Colony count at 30 C by the pour plate technique OVERLAY MEDIUM AGAR BIOS LL ISO :2013 Colony count at 30 degrees C by the surface plating technique PLATE COUNT AGAR (PCA)+SKIMMED MILK PLATE COUNT AGAR WITH SKIM MILK ENUMERATION OF PSICOTHROPHIC MICRORGANISMS PLATE COUNT MEDIUM TRYPTIC GLUCOSE YEAST AGAR ISO 17410:2001 Enumeration BACILLUS CEREUS MYP AGAR BACILLUS CEREUS AGAR BASE MYP ISO 7932:2004 Colony-count technique at 30 C BACILLUS CEREUS ANTIMICROBIC SUPPL EGG YOLK EMULSION BACILLUS CEREUS SELECTIVEAGAR MYP SHEEP BLOOO AGAR BLOOD AGAR BASE N Biolife Italiana 1/11

2 BACILLUS CEREUS TRYPTO SOYA POLYMYXIN BROTH (TSPB) TRYPTIC SOY BROTH ISO 21871:2006 Method for the determination of low numbers of presumptive Bacillus cereus Most probable number technique and detection method BACILLUS CEREUS ANTIMICROBIC SUPPL POLYMYXIN PYRUVATE EGG YOLK MANNITOL BROMOTHYMOL BLUE AGAR BACILLUS CEREUS AGAR BASE PEMBA BACILLUS CEREUS ANTIMICROBIC SUPPL EGG YOLK EMULSION BACILLUS CEREUS SELECTIVEAGAR PEMBA MANNITOL EGG YOLK POLYMYXIN AGAR (MYP) BACILLUS CEREUS AGAR BASE MYP BACILLUS CEREUS ANTIMICROBIC SUPPL EGG YOLK EMULSION BACILLUS CEREUS SELECTIVEAGAR MYP SHEEP BLOOO AGAR TRYPTIC SOY AGAR BLOOD AGAR SHEEP BROCHOTHRIX THERMOSPHACTA STAA STAA AGAR BASE ISO 13969:2003 IDF 183:2003 STAA SELECTIVE SUPPLEMENT OXIDASE TEST OXIDASE TEST STRIPS CAMPYLOBACTER BOLTON BROTH CAMPYLOBACTER ENRICH. BASE BROTH-BOLTON B ISO :2006 Detection method LYSED HORSE BLOOD 90HLX100 BOLTON BROTH SUPPLEMENT MODIFIED CHARCOAL CEFOPERAZONE DEOXYCHOLATE AGAR (MCCD AGAR) CAMPY. BLOOD FREE MEDIUM BASE BOLTON BOLTON CCDA ANTIMICROBRIC SUPPL CAMPYLOBACTER BLOOD FREE AGAR (CCDA) SECOND SELECTIVE PLATEING MEDIUM CAMPY. BLOOD FREE MEDIUM BASE KARMALI KARMALI SELECTIVE SUPPLEMENT CAMPYLOBACTER BLOOD FREE AGAR KARMALI COLUMBIA BLOOD AGAR COLUMBIA BLOOD AGAR BASE COLUMBIA BLOOD AGAR SHEEP BRUCELLA BROTH BRUCELLA BROTH (ALBIMI) MUELLER HINTON BLOOD AGAR MUELLER HINTON AGAR II MUELLER HINTON BLOOD AGAR SHEEP CAMPYLOBACTER MODIFIED CHARCOAL CEFOPERAZONE DEOXYCHOLATE AGAR (MCCD AGAR) CAMPY. BLOOD FREE MEDIUM BASE BOLTON ISO/TS :2006 Colony count method BOLTON CCDA ANTIMICROBRIC SUPPL CAMPYLOBACTER BLOOD FREE AGAR (CCDA) COLUMBIA BLOOD AGAR COLUMBIA BLOOD AGAR BASE COLUMBIA BLOOD AGAR SHEEP BRUCELLA BROTH BRUCELLA BROTH (ALBIMI) Biolife Italiana 2/11

3 CAMPYLOBACTER BOLTON BROTH CAMPYLOBACTER ENRICH. BASE BROTH-BOLTON B ISO/TS :2010 Semi quantitative detection LYSED HORSE BLOOD 90HLX100 BOLTON BROTH SUPPLEMENT MODIFIED CHARCOAL CEFOPERAZONE DEOXYCHOLATE AGAR (MCCD AGAR) CAMPY. BLOOD FREE MEDIUM BASE BOLTON BOLTON CCDA ANTIMICROBRIC SUPPL CAMPYLOBACTER BLOOD FREE AGAR (CCDA) COLUMBIA BLOOD AGAR COLUMBIA BLOOD AGAR BASE COLUMBIA BLOOD AGAR SHEEP BRUCELLA BROTH BRUCELLA BROTH (ALBIMI) MUELLER HINTON BLOOD AGAR MUELLER HINTON AGAR II MUELLER HINTON BLOOD AGAR SHEEP CLOSTRIDIUM PERFRINGENS SULFITE-CYCLOSERINE AGAR (SC) TSC AGAR BASE ISO 7937:2004 Colony count technique D-CYCLOSERINE ANTIMICROBIC SUPPL FLUID THIOGLYCOLLATE MEDIUM THIOHLYCOLLATE MEDIUM USP LACTOSE SULFITE MEDIUM (LS) LACTOSE SULFITE MEDIUM NITRATE MOTILITY MEDIUM MOTILITY NITRATE CP MEDIUM LACTOSE GELATIN MEDIUM LACTOSE GELATIN MEDIUM COLIFORMS CRYSTAL VIOLET NEUTRAL RED BILE LACTOSE (VRBL) AGAR VIOLET RED BILE AGAR ISO 4832:2006 Colony-count technique BRILLIANT GREEN LACTOSE BILE BROTH BRILLIANT GREEN BILE BROTH 2% COLIFORMS LAURYL SULFATE TRYPTOSE BROTH LAURYL PEPTO BIOS BROTH ISO 4831:2006 Most probable number tecnique BRILLIANT GREEN LACTOSE BILE BROTH BRILLIANT GREEN BILE BROTH 2% CRONOBACTER SAKAZAKII MILK AND MILK PRODUCBUFFERED PEPTONE WATER (BPW) BUFFERED PEPTONE WATER ISO/TS 22964:2006 IDF/RM 210: 2006 Detection MODIFIED LAURYL SULFATE TRYPTOSE ENTEROBACTER SAKAZAKII SELECTIVE BROTH (mlst) VANCOMYCIN MEDIUM BROTH (ESSB) ISO FORMULATION VANCOMYCIN ANTIMICROBIC SUPPLEMENT ENTEROBACTER SAKAZAKII ENTEROBACTER SAKAZAKII ISOLATION AGAR (ESIA) ISOLATION AGAR (ESIA) ESCHERICHIA COLI LAURYL SULFATE BROTH LAURYL PEPTO BIOS BROTH ISO 7251:2005 Most probable number technique EC BROTH EC BROTH PEPTONE WATER INDOLE FREE PEPTONE-TRYPTONE WATER INDOLE REAGENT KOVACS' REAGENT E.COLI β-glucuronidase POSITIVE MINERALS MODIFIED GLUTAMATE MEDIUM MINERALS MODIFIED GLUTAMATE MEDIUM ISO :2001 Colony-count technique at 44 degrees C using membranes SODIUM GLUTAMATE TRYPTONE BILE GLUCURONIDE AGAR TRYPTONE BILE X-GLUC AGAR (TBX) E.COLI β-glucuronidase POSITIVE TRYPTONE BILE GLUCURONIDE AGAR TRYPTONE BILE X-GLUC AGAR (TBX) ISO :2001 Colony-count technique at 44 degrees Biolife Italiana 3/11

4 E.COLI β-glucuronidase POSITIVE MINERALS MODIFIED GLUTAMATE MEDIUM MINERALS MODIFIED GLUTAMATE MEDIUM ISO :2015 Detection and most probable number technique SODIUM GLUTAMATE TRYPTONE BILE GLUCURONIDE AGAR TRYPTONE BILE X-GLUC AGAR (TBX) ESCHERICHIA COLI O157 MODIFIED TRYPTONE SOYA BROTH WITHNOVOBIOCIN TRYPTIC SOY BROTH MODIFIED (m-tsb) M ISO 16654:2001 Detection NOVOBIOCIN ANTIMICROBIC SUPPLEMENT TC-SMAC MAC CONKEY SORBITOL AGAR S CEFIXIME TELLURITE O157 SUPPLEMENT 42ISEC NUTRIENT AGAR NUTRIENT AGAR w/ NaCl TRYPTONE TRYPTOPHANE MEDIUM TRYPTONE TRYPTOPHANE MEDIUM INDOLE REAGENT KOVACS' REAGENT ENTEROBACTERIACEAE BUFFERED PEPTONE WATER (DILUENT) BUFFERED PEPTONE WATER ISO :2004 Detection and enumeration by MPN technique with pre-enrichment BUFFERED BRILLIANT GREEN BILE GLUCOSE BROTH (EE BROTH) EE BROTH MOSSEL VIOLET RED BILE GLUCOSE (VRBG) AGAR VIOLET RED BILE GLUCOSE AGAR NUTRIENT AGAR NUTRIENT AGAR w/ NaCl GLUCOSE AGAR PURPLE GLUCOSE AGAR ENTEROBACTERIACEAE VIOLET RED BILE GLUCOSE (VRBG) AGAR VIOLET RED BILE GLUCOSE AGAR ISO :2004 Colony-count method NUTRIENT AGAR NUTRIENT AGAR w/ NaCl GLUCOSE AGAR PURPLE GLUCOSE AGAR LACTOBACILLUS ACIDOPHILUS MRS/CLINDAMYCIN/CIPROFLOXACIN AGAR (MRS/CL/CIP AGAR) MRS AGAR WITH TWEEN ISO 20128:2006 IDF 192:2006 Colony-count technique at 37 C CLYNDAMICIN AND CIPROFLOXACIN LISTERIA MONOCYTOGENES HALF FRASER BROTH FRASER BROTH BASE ISO :1996 / Amd 1:2004 Detection FRASER HALF SELECTIVE SUPPLEMENT LISTERIA FRASER HALF BROTH FRASER BROTH FRASER BROTH BASE FRASER SELECTIVE SUPPLEMENT LISTERIA FRASER BROTH AGAR LISTERIA ACC.TO OTTAVIANI AGOSTI (ALOA) AGAR LISTERIA ACC.TO OTTAVIANI AGOSTI (ALOA ) ALOA SELECTIVE ENRICHMENT SUPPL ALOA AGAR LISTERIA ACC.TO OTTAVIANI AGOSTI SECOND SELECTIVE MEDIUM LISTERIA PALCAM AGAR BASE PALCAM SELETIVE SUPPLEMENT LISTERIA SELECTIVE AGAR (PALCAM) or LISTERIA OXFORD AGAR BASE OXFORD ANTIMICROBIC SUPPLEMENT LISTERIA SELECTIVE AGAR (OXFORD) TSYEA TRYPTIC SOY YEAST EXTRACT AGAR TSYEB TRYPTIC SOY YEAST EXTRACT BROTH Biolife Italiana 4/11

5 SHEEP BLOOD AGAR BLOOD AGAR BASE N CARBOHYDRATE UTILISATION BROTH FERMENTATION BROTH BASE MOTILITY AGAR PHOSPHATE BUFFERED SALINE LISTERIA MONOCYTOGENES BUFFERED PEPTONE WATER or BUFFERED PEPTONE WATER or ISO :1998 / Amd 1:2004 Enumeration BASE MEDIUM FOR HALF-FRASER BROTH WITH AMMONIUM IRON(III) CITRATE LISTERIA FRASER BROTH BASE HALF CONCENTRATION LISTERIA FRASER SUPPLEMENT (FERRIC AMMONIUM CITRATE) AGAR LISTERIA ACC.TO OTTAVIANI AGOSTI (ALOA) AGAR LISTERIA ACC.TO OTTAVIANI AGOSTI (ALOA ) ALOA SELECTIVE ENRICHMENT SUPPL ALOA AGAR LISTERIA ACC.TO OTTAVIANI AGOSTI TSYEA TRYPTIC SOY YEAST EXTRACT AGAR TSYEB TRYPTIC SOY YEAST EXTRACT BROTH SHEEP BLOOD AGAR BLOOD AGAR BASE N CARBOHYDRATE UTILISATION BROTH FERMENTATION BROTH BASE MOTILITY AGAR PHOSPHATE BUFFERED SALINE MESOPHILIC LACTIC ACID BACTERIA ISO 15214:1998 Colony count MRS MEDIUM (DE MAN, ROGOSA AND SHARPE) AT PH 5,7 MRS AGAR WITH TWEEN 80 (ph to be adjusted) PSEUDOMOS - MEAT AND MEAT PRODUCTS CEPHALOTHIN-SODIUM FUSIDATE-CETRIMIDE AGPSEUDMOS AGAR BASE ISO 13720:2010 Enumeration of presumptive Pseudomonas spp CFC PSEUDOMOS SUPPLEMENT OXIDASE TEST OXIDASE TEST STRIPS PSEUDOMOS - MILK AND MILK PRODUCTS PENICILLIN AND PIMARICIN AGAR (PPA) PSEUDMOS AGAR BASE / ISO/TS 11059:2009 IDF/RM 225:2009 Method for the enumeration of Pseudomonas spp. PP PSEUDOMOS SUPPLEMENT GLUCOSE AGAR PURPLE GLUCOSE AGAR OXIDASE TEST OXIDASE TEST STRIPS SALMONELLA BUFFERED PEPTONE WATER BUFFERED PEPTONE WATER ISO 6579:2002 Detection RAPPAPORT-VASSILIADIS MEDIUM WITH SOYA (RVS BROTH) RAPPAPORT VASSILIADIS SOY (RVS) BROTH MULLER-KAUFFMANN TETRATHIOTE- NOVOBIOCIN BROTH (MKTTN) MUELLER KAUFFMANN TETRATHIOTE BROTH BASE ISO FORMULATION NOVOBIOCIN MKTT SELECTIVE SUPPLEMENT IODINE SOLUTION MUELLER KAUFFMANN TETRATHIOTE NOVOBIOCIN BROTH XYLOSE LYSINE DEOXYCHOLATE AGAR (XLD AGAR) XLD AGAR ISO FORMULATION Biolife Italiana 5/11

6 2nd PLATING MEDIUM CHROMOGENIC SALMONELLA AGAR BASE SALMONELLA SELECTIVE SUPPLEMENT CHROMOGENIC SALMONELLA AGAR TSI AGAR TRIPLE SUGAR IRON AGAR ISO FORM S UREA AGAR (CHRISTENSEN) UREA AGAR BASE CHRISTENSEN UREA 40% SOLUTION UREA AGAR L-LYSINE DECARBOXYLASE MEDIUM - VP MEDIUM MR VP MEDIUM ALPHA PHTOL 6% SOLUTION TRYPTONE TRYPROPHAN MEDIUM TRYPTONE TRYPTOPHAN MEDIUM INDOLE REAGENT KOVACS' REAGENT SEMISOLID NUTRIENT AGAR SALMONELLA BUFFERED PEPTONE WATER BUFFERED PEPTONE WATER ISO 6579:2002/Amd 1:2007 Detection in animal faeces and environmental samples MODIFIED SEMI-SOLID RAPPAPORT- VASSILIADIS MEDIUM (MSRV) MSRV MEDIUM BASE NOVOBIOCIN ANTIMICROBIC SUPPLEMENT (10 mg/l) XLD AGAR XLD AGAR ISO FORMULATION nd PLATING MEDIUM CHROMOGENIC SALMONELLA AGAR BASE SALMONELLA SELECTIVE SUPPLEMENT CHROMOGENIC SALMONELLA AGAR SALMONELLA BUFFERED PEPTONE WATER BUFFERED PEPTONE WATER ISO/TS :2012 Enumeration by a miniaturized most MODIFIED SEMI-SOLID RAPPAPORTprobable number technique VASSILIADIS MEDIUM (MSRV) MSRV MEDIUM BASE NOVOBIOCIN ANTIMICROBIC SUPPLEMENT (10 mg/l) XLD AGAR XLD AGAR ISO FORMULATION nd PLATING MEDIUM CHROMOGENIC SALMONELLA AGAR BASE SALMONELLA SELECTIVE SUPPLEMENT CHROMOGENIC SALMONELLA AGAR TSI AGAR TRIPLE SUGAR IRON AGAR ISO FORM S UREA AGAR (CHRISTENSEN) UREA AGAR BASE CHRISTENSEN UREA 40% SOLUTION UREA AGAR L-LYSINE DECARBOXYLASE MEDIUM - SHIGELLA SHIGELLA BROTH SHIGELLA BROTH BASE ISO 21567:2004 Detection NOVOBIOCIN ANTIMICROBIC SUPPLEMENT MAC CONKEY AGAR MAC CONKEY AGAR XLD AGAR XLD AGAR ISO FORMULATION HEKTOEN ENTERIC AGAR HEKTOEN ENTERIC AGAR TSI AGAR TRIPLE SUGAR IRON AGAR ISO FORMULAT S Biolife Italiana 6/11

7 SEMISOLID NUTRIENT AGAR UREA AGAR (CHRISTENSEN) UREA AGAR BASE CHRISTENSEN UREA 40% SOLUTION UREA AGAR L-LYSINE DECARBOXYLASE MEDIUM L-ORNITHINE DECARBOXYLASE MEDIUM TRYPTONE DL TRYPTOPHANE MEDIUM BROMOCRESOLE PURPLE BROTH SODIUM ACETATE AGAR CHRISTEMNSEN CITRATE AGAR MUCATE BROTH CONTROL BROTH SULFITE REDUCING BACTERIA IRON SULFITE AGAR TSC AGAR BASE ISO 15213:2003 Enumeration STAPHYLOCOCCI -coagulase positive BAIRD PARKER AGAR MEDIUM BAIRD PARKER AGAR BASE ISO :1999 / Amd 1:2003 Enumeration technique using Baird-Parker agar EGG YOLK TELLURITE EMULSION 20% BAIRD PARKER EYT AGAR BRAIN HEART INFUSION BROTH BRAIN HEART INFUSION BROTH RABBIT PLASMA COAGULASE PLASMA EDTA STAPHYLOCOCCI -coagulase positive RABBIT PLASMA FIBRINOGEN AGAR MEDIUM BAIRD PARKER AGAR BASE ISO :1999 /Amd 1:2003 Enumeration technique using RPF agar RPF SUPPLEMENT II STAPHYLOCOCCI -coagulase positive MODIFIED GIOLITTI AND CANTONI BROTH GIOLITTI-CANTONI BROTH ISO :2003 Detection and MPN technique for low numbers POTASSIUM TELLURITE 1% SOLUTION AGAR SOLUTION AGAR BIOS LL BAIRD PARKER AGAR MEDIUM BAIRD PARKER AGAR BASE EGG YOLK TELLURITE EMULSION 20% SULFAMEZATHINE SELECTIVE SUPPL BAIRD PARKER AGAR RABBIT PLASMA FIBRINOGEN AGAR MEDIUM BAIRD PARKER AGAR BASE RPF SUPPLEMENT II BAIRD PARKER RPF AGAR BRAIN HEART INFUSION BROTH BRAIN HEART INFUSION BROTH RABBIT PLASMA COAGULASE PLASMA EDTA Biolife Italiana 7/11

8 VIBRIO ALKALINE SALINE PEPTONE WATER ALKALINE PEPTONE WATER ISO/TS :2007 Detection of Vibrio parahaemolyticus and Vibrio cholerae THIOSULFATE, CITRATE, BILE AND SUCROSE AGAR (TCBS) TCBS KOBAYASHI AGAR SALINE NUTRIENT AGAR S SALINE TRIPLE SUGAR IRON (TSI) AGAR TRIPLE SUGAR IRON AGAR ISO FORM. + SODIUM CHLORIDE S SALINE MEDIUM FOR DETECTION OF ORNITHINE DECARBOXYLASE (ODC) SALINE MEDIUM FOR DETECTION OF LYSINE DECARBOXYLASE (LDC) SALINE MEDIUM FOR DETECTION OF ARGININE DEHYDROXYLASE (ADH) BUFFER ONPG SOLUTION SALINE MEDIUM FOR DETECTION OF INDOLE KOVACS' REAGENT SALINE PEPTONE WATERS DICHLORAN-ROSE BENGAL YEASTS AND MOULDS CHLORAMPHENICOL AGAR (DRBC) DRBC AGAR BASE ISO :2008 Colony count technique in products with water activity greater than 0,95 CHLORAMPHENICOL ANT SUPPLEMENT YEASTS AND MOULDS DICHLORAN 18 % MASS FRACTION GLYCEROL AGAR (DG18) DG18 AGAR BASE ISO :2008 Colony count technique in products with water activity less than or equal to 0,95 GLYCEROL CHLORAMPHENICOL ANT SUPPLEMENT YERSINIA ENTEROCOLITICA PSB BROTH YERSINIA PSB BROTH ISO 10273:2003 Detection of presumptive pathogenic Yersinia enterocolitica ITC BROTH YERSINIA ITC BROTH BASE TICARCILLIN IRGASAN ANTIMICROBIC SUPP POTASSIUM CHLORATE SUPPLEMENT CIN AGAR CIN AGAR BASE YERSINIA SELECTIVE SUPPLEMENT YERSINIA SELECTIVE AGAR SSDC UREA INDOLE KLIGLER IRON AGAR KLIGLER IRON AGAR MEDIUM FOR LYSINE DECARBOXYLASE MEDIUM FOR ORNITHINE DECARB. FERMENTATION BROTH SIMMONS CITRATE AGAR TWEEN ESTERASE MEDIUM AGAR : NOT AVAILABLE WITHIN BIOLIFE PRODUCTS RANGE ALOA IS A TRADEMARK OF BIOLIFE ITALIA Biolife Italiana 8/11

9 EQUIVALENCE TABLE BETWEEN ISO RECOMMENDED CULTURE MEDIA AND BIOLIFE CULTURE MEDIA MICROBIOLOGICAL METHODS FOR WATER ALYSIS rev 6-06/2016 APPLICATION CAMPYLOBACTER COMPLETE PRESTON BROTH CAMPYLOBACTER ENRICHMENT BROTH BASE (PRESTON) ISO 17995:2005 Detection and enumeration PRESTON ANTIMICROBIC SUPPLEMENT II CAMPYLOBACTER GROWTH SUPPLEMENT LYSED HORSE BLOOD 90HLX100 COMPLETE BOLTON BROTH CAMPY. ENRICH. BASE BROTH-BOLTON BOLTON BROTH SUPPLEMENT COMPLETE MODIFIED-CHARCOAL CEFOPERAZONE DESOXYCHOLATE AGAR (m- CCDA) CAMPY. BLOOD FREE MEDIUM BASE BOLTON BOLTON CCDA ANTIMICROBRIC SUPPL CAMPYLOBACTER BLOOD FREE AGAR (CCDA) CLOSTRIDIUM PERFRINGENS TRYPTOSE SULFITE-CYCLOSERINE AGAR (SC) TSC AGAR BASE ISO 14189:2013 Enumeration by membrane filtration method D-CYCLOSERINE ANTIMICROBIC SUPPL BLOOD AGAR COLUMBIA BLOOD AGAR ACID PHOSPHATASE REAGENT CULTURABLE MICROORGANISM YEAST EXTRACT AGAR YEAST EXTRACT AGAR ISO 6222:1999 Colony count ENTEROCOCCI SLANETZ BARTLEY AGAR SLANETZ BARTLEY AGAR ISO :2000- Detection and enumeration of intestinal enterococci by membrane filtration method TTC 1% SOLUTION BILE AESCULIN AZIDE AGAR BILE AESCULIN AZIDE AGAR ISO FORM ESCHERCHIA COLI AND COLIFORM BACTERIA CHROMOGENIC COLIFORM AGAR (CCA) CHROMOGENIC COLIFORM AGAR ISO FORM ISO :2014 Enumeration by membrane filtration method TRYPTONE SOYA AGAR TRYPTIC SOY AGAR ESCHERCHIA COLI AND COLIFORM BACTERIA LACTOSE BROTH LACTOSE BROTH ISO :2012 enumeration by Most probable number method MAC CONKEY BROTH MAC CONKEY BROTH PURPLE IMPROVED FORMATE LACTOSE GLUTAMATE MEDIUM MINERALS MODIFIED GLUTAMATE MEDIUM SODIUM GLUTAMATE LAURYL TRYPTOSE (LACTOSE BROTH) LAURYL PEPTO BIOS BROTH BRILLIANT GREEN LACTOSE (BILE) BROTH BRILLIANT GREEN BILE BROTH 2% EC MEDIUM EC BROTH TRYPTONE WATER PEPTONE-TRYPTONE WATER LAURYL TRYPTOSE MANNITOL BROTH WITH TRYPTOPHAN NUTRIENT AGAR Biolife Italiana 9/11

10 LEGIONELLA BUFFERED CHARCOAL YEAST EXTRACT AGAR MEDIUM (BCYE) LEGIONELLA BCYE AGAR BASE ISO 11731:1998 Detection and enumeration LEGIONELLA BCYE α-growth SUPPL LEGIONELLA AGAR BUFFERED CHARCOAL YEAST EXTRACT MEDIUM WITHOUT L-CYSTEINE (BCYE - CYS) LEGIONELLA BCYE AGAR BASE LEGIONELLA BCYE α-growth SUPPL. W/O CYSTEINE LEGIONELLA AGAR W/O CYSTEINE BUFFERED CHARCOAL YEAST EXTRACT MEDIUM WITH SELECTIVE SUPPLEMENTS (GVPC MEDIUM) LEGIONELLA BCYE AGAR BASE LEGIONELLA BCYE α-growth SUPPL LEGIONELLA GVPC SELECTIVE SUPPL LEGIONELLA SELECTIVE AGAR ACID BUFFER PAGE'S SALINE DILUTE RINGER'S SOLUTION. PHOSPHATE-BUFFERED SALINE FORMOL SALINE. SEROLOGICAL REAGENTS LEGIONELLA RAPID LATEX TEST LEGIONELLA BUFFERED CHARCOAL YEAST EXTRACT AGAR MEDIUM (BCYE) LEGIONELLA BCYE AGAR BASE ISO :2004 Direct membrane filtration method for waters with low bacterial counts LEGIONELLA BCYE α-growth SUPPL LEGIONELLA AGAR BUFFERED CHARCOAL YEAST EXTRACT MEDIUM WITHOUT L-CYSTEINE (BCYE - CYS) LEGIONELLA BCYE AGAR BASE LEGIONELLA BCYE α-growth SUPPL. W/O CYSTEINE LEGIONELLA AGAR W/O CYSTEINE BUFFERED CHARCOAL YEAST EXTRACT MEDIUM WITH SELECTIVE SUPPLEMENTS (GVPC MEDIUM) LEGIONELLA BCYE AGAR BASE LEGIONELLA BCYE α-growth SUPPL LEGIONELLA GVPC SELECTIVE SUPPL LEGIONELLA SELECTIVE AGAR GLYCEROL BROTH NUTRIENT BROTH GLYCEROL ACID BUFFER PAGE'S SALINE Biolife Italiana 10/11

11 PSEUDOMOS AERUGINOSA PSEUDOMOS AGAR BASE/CN-AGAR PSEUDOMOS AGAR BASE ISO 16266:2006 Detection and enumeration - Method by membrane filtration CN PSEUDOMOS SUPPLEMENT GLYCEROL PSEUDOMOS CN SELECTIVE AGAR KING'S B MEDIUM PSEUDOMOS AGAR F ACETAMIDE BROTH ACETAMIDE BROTH OXIDASE TEST OXIDASE TEST STRIPS NESSLER REAGENT SALMONELLA BUFFERED PEPTONE WATER BUFFERED PEPTONE WATER ISO 19250:2010 Detection RAPPAPORT-VASSILIADIS MEDIUM WITH SOYA (RVS BROTH) RAPPAPORT VASSILIADIS SOY (RVS) BROTH XYLOSE LYSINE DEOXYCHOLATE AGAR (XLD AGAR) XLD AGAR ISO FORMULATION TRIPIE SUGAR/IRON AGAR (TSI AGAR) TRIPLE SUGAR IRON AGAR ISO FORM S UREA AGAR (CHRISTENSEN) UREA AGAR BASE CHRISTENSEN UREA 40% SOLUTION UREA AGAR L-LYSINE DECARBOXYLASE MEDIUM - SELENITE CYSTINE BROTH SELENITE CYSTINE BROTH MULLER-KAUFFMANN TETRATHIOTE- NOVOBIOCIN BROTH (MKTTN) MUELLER KAUFFMANN TETRATHIOTE BROTH BASE ISO FORMULATION NOVOBIOCIN MKTT SELECTIVE SUPPLEMENT IODINE SOLUTION MUELLER KAUFFMANN TETRATHIOTE NOVOBIOCIN BROTH SULFITE REDUCING AEROBES CLOSTRIDIA DIFFERENTIAL REINFORCED CLOSTRIDIAL MEDIUM ISO :1986 Method by enrichment in liquid medium SULFITE REDUCING AEROBES CLOSTRIDIA SULPHITE IRON AGAR or ISO :1986 Method by membrane filtration TRYPOSE-SULFITE AGAR TSC AGAR BASE : NOT AVAILABLE WITHIN BIOLIFE PRODUCTS RANGE *: BIOLIFE FORMULATION DOESN'T COMPLETELEY CORRESPOND TO ISO FORMULATION Biolife Italiana 11/11