Quality Evaluation Laboratory, Spices Board,Cochin.
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1 PHYSICO-CHEMICAL ANALYSIS 1 Agmark specifications 500 * > 2 ASTA specifications 500 / 250X 10 Nos *** * 1 3 Acid insoluble ash Added Starch Alcohol soluble extract Bulk density / Litre weight of spices Artificial colourants in Cardamom Capsaicin - HPLC method (%/SHU) Calcium as Calcium Oxide Coumarin Content Chromate test (Qualitative) Cold water soluble extract Colour value (ASTA METHOD) Common salt Crocine Crude fibre Curcumin (ASTA METHOD) Excreta, mamalian 500/ Excreta, others 500/ Extraneous / foreign matter 500 / Filth, heavy Filth, light 100 # Gingerols and Shogaols Insect defiled / infested 500 / Light berries Moisture (ASTA METHOD) Mould (Physical) Non - volatile ether extract Oleoresin (EDC extractables) *Oleoresin(EDC extractables in Black Pepper)[ISO 1108 Method] Other Seeds HN_GV 1 of 5
2 32 Other Seeds in Cumin Picrocrocine Piperine (ASTA METHOD) *Piperine content[iso 5564 Method] Safranal Starch Sulphur dioxide Total ash USFDA specifications 500/250x10 nos * 6 41 Volatile oil Vanillin Vanillic acid P-Hydroxybenzoic acid P-Hydroxybenzaldehyde Water activity Water soluble ash Whole insects,dead (by count) RESIDUAL ANALYSIS 49 Aflatoxin(B1,B2,G1,G2) [HPLC -METHOD] Heavy metals (Cd, Cr, Cu, Fe, Mg, Mo, Pb & Zn ) ## 5 51 Heavy Metal : Arsenic Heavy Metal : Mercury Mineral oil in Black Pepper Ochratoxin A [HPLC-METHOD] Orange II Organochlorine Pesticides Residues (Isomers of BHC, Endosulfan and DDT; Heptachlor, Aldrin, Dieldrin, Endrin and Endrin aldehyde) 57 Organochlorine Pesticide Residue - Dicofol Organophosphorous Pesticides Residues - (I) (Chlorpyriphos,Dimethoate, Disulfoton,Ethion, Methyl Parathion, Phorate, Parathion and Quinalphos) 59 Organophosphorus Pesticides Residues (II) ( Methyl Pirimiphos, Phosalone, Monocrotophos) HN_GV 2 of 5
3 60 Pesticide Residues-III (Iprobenphos,Triazophos and Profenphos) 61 Pesticide Residues-IV (Ethion,Iprobenphos,Triazophos and Profenphos) 62 Pesticide Residues-V (Profenphos,Bifenthrin,Triazophos,Diethion and Endosulfan) 63 Pesticide Residues-VI (Ethion,Iprobenphos,Triazophos,Profenophos,Phorat e,parathion,chlorpyriphos,methyl Parathion 64 * Pesticide Residues -VII (Profenofos,Triazophos,Endosulfans) 65 Pyrethroid Pesticides Residues (Cypermethrin and Fenvalerate) Pesticide Residue-Malathion Pesticide Residue-Phorate Pesticide Residue-Etrimphos Pesticide Residue-Fenitrothion Pesticide Residue-Phosmet Pesticide Residue-Chlorfenvinphos Pesticide Residue-Parathion Pesticide Residue-Lambda Cyhalothrin Pesticide Residue-Chlorothalonil Pesticide Residue-Azinphos Ethyl Pesticide Residue-Alachlor Pesticide Residue- Aldicarb Pesticide Residue-Carbofuran Pesticide Residue-Methomyl Pesticide Residue-O Methoate Pesticide residues. single compound (any of the above) Sudan - Dye (I,II,III & IV)[HPLC METHOD]** Sudan - Dye (I,II,III & IV)[LC-MS/MS METHOD] Synthetic Dyes II (Sudan Red 7b,Sudan Orange G, Para Red,Rhodamine B and Butter Yellow (LC-MS/MS METHOD)) Synthetic Dyes II - Single compound(any of the above) Sunset Yellow FCF in Sugar Coated Fennel HN_GV 3 of 5
4 MICROBIOLOGICALANALYSIS 87 Aerobic Plate Count Aerobic Spore Count Bacillus cereus Bacillus cereus (AOAC Performance Tested ) Clostridium perfringens Coliforms Coliforms (AOAC Performance Tested ) Enterobacteriacea Enterobacteriacea(AOAC Performance Tested ) E.coli E.coli(AOAC Method) Mould Salmonella (FDA BAM) Salmonella(AOAC OMA Method) Staphylococcus aureus Staphylococcus aureus (AOAC Performance Tested ) Sulphite Reducing Clostridia Thermostable Bacteria Total Viable Count(AOAC Method) Yeast Yeast and Mould Yeast and Mould (AOAC Performance Tested ) HN_GV 4 of 5
5 Note:- * 1.Form no 75 Revised as on with addition of Parameters given under *. 2.Microbiological parameters by AOAC method/aoac Performance tested TEMPO method(automated,fast analytical,validated methods which are internationally accepted).for further technical details on AOAC Method,please contact ID sbqel@indianspices.com 3. Analytical Charges revised with effect from Service will be charged extra as per Government norms. 4. > Depends on the number of parameters for individual spices and number of samples. 5. * Charges based on the parameter specified for each spice. 6. **Turmeric is analysed for Sudan I-IV by HPLC Method unless otherwise specified. 7. ***In the case of analysis for ASTA / USFDA parameters, sample size should be a minimum of 500 g for heavier items like pepper,ginger,tumeric etc. and 250 g sample for low density items like chilli and seed spices. 8. # Quantity for light filth analysis for Tamarind concentrate by AOAC method should be a minimum of 600g. 9. ##Analytical charges Rs. 500/- per element. In the case of analysis of oleoresin samples of spices for the above parameters a minimum of 50 g sample size is required and in case of coriander and herbs a sample size of 300 g is required. 11. The days required for the analysis will increase depending on the inflow of samples for analysis. The samples may be sent to the Scientist D & Head, Quality Evaluation Laboratory, Spices Board, Sugandha Bhavan, Palarivattom, Kochi ,Kerala, along with analytical charges in the form of D.D. drawn in favour of Secretary, Spices Board,Kochi-25. HN_GV 5 of 5
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