TUV SUD SOUTH ASIA PVT. LTD. A1, INDUSTRIAL ESTATE OPPOSITE TO NIRMAN PARK KANCHARAPALEM MAIN ROAD, VISAKHAPATNAM ANDHRA PRADESH INDIA

Transcription

1 This is to attest that TUV SUD SOUTH ASIA PVT. LTD. A1, INDUSTRIAL ESTATE OPPOSITE TO NIRMAN PARK KANCHARAPALEM MAIN ROAD, VISAKHAPATNAM ANDHRA PRADESH INDIA Testing Laboratory TL-703 has met the requirements of the IAS Accreditation Criteria for Testing Laboratories (AC89), has demonstrated compliance with ISO/IEC Standard 17025:2005, General requirements for the competence of testing and calibration laboratories, and has been accredited, commencing May 30, 2016, for the test methods listed in the approved scope of accreditation. This certificate expires June 1, (See laboratory s scope of accreditation for fields of testing and accredited test methods.) This accreditation certificate supersedes any IAS accreditation bearing an earlier effective date. The certificate becomes invalid upon suspension, cancellation or revocation of accreditation. See for current accreditation information, or contact IAS at C.P. Ramani, P.E., C.B.O President

2 IAS Accreditation Number Accredited Entity Address TL-703 TUV SUD South Asia PVT. LTD. A1, Industrial Estate Opposite to Nirman Park Kancharapalem Main Road, Visakhapatnam Andhra Pradesh India Dr. Shaik Ali Contact Name Telephone Effective Date of Scope May 30, 2016 Accreditation Standard ISO/IEC Standard 17025:2005 FIELDS OF TESTING MATERIAL DETERMINANTS METHOD REFERENCE Microbiology Fish and Marine Aerobic Plate Count Chapter 3, US FDA - BAM IS 5402 ISO ISO Escherichia coli Chapter 4, US FDA - BAM IS 5887 (P 1) ISO ISO Enumeration of Fecal Coliforms Chapter 4, US FDA BAM Enumeration of Coliforms Chapter 4, US FDA - BAM IS 5401 (P 1) ISO 4832 ISO 4831 Shigella spp Chapter 6, US FDA - BAM IS 5887 (P 7) Staphylococcus aureus Chapter 12, US FDA - BAM IS 5887 (P 8 / Sec 1) IS 5887 (P 2) ISO 6888 (P 1) Salmonella spp IS 5887 (P 3) Chapter 5, US FDA - BAM ISO , AOAC Yeast and Mold Count Chapter 18, US US FDA - BAM IS 5403 Bacillus cereus Chapter 14, US FDA BAM IS 5887 (P 6) ISO 7932 Listeria monocytogenes Chapter 10, US FDA - BAM IS (P 1) ISO ISO , AOAC Enterobacteriaceae ISO Enterococci Chapter 10, APHA 5 th Edition Page 2 of 27

3 Microbiology Fish and Marine Clostridium perfringens Chapter 16, US FDA - BAM IS 5887 (P 4) ISO 7937 Vibrio cholerae Chapter 9, US FDA - BAM IS 5887 (P 5) ISO Vibrio parahaemolyticus Chapter 9, US FDA - BAM IS 5887 (P 5) ISO Vibrio vulnificus Chapter 9, US FDA - BAM ISO Sulfite reducing bacteria / ISO Sulphide spoilage spore formers Meat & Meat Aerobic plate count Chapter 3, US FDA - BAM IS 5402 ISO & 2 Escherichia coli Chapter 4, US FDA - BAM IS 5887 (P 1) Enumeration of Fecal Coliforms Chapter 4, US FDA - BAM Enumeration of Coliforms Chapter 4, US FDA - BAM IS 5401 (P 1) Staphylococcus aureus Chapter 12, US FDA - BAM IS 5887 (P 8 / Sec 1) IS 5887 (P 2) Salmonella spp IS 5887 (P 3) Chapter 5, US FDA - BAM , AOAC Yeast and Mold count Chapter 18, US FDA - BAM IS 5403 Listeria monocytogenes Chapter 10, US FDA - BAM IS (P 1) , Chapter 17, AOAC Clostridium perfringens ISO 7937 IS 5887 (P 4) Shigella spp IS 5887 (P 7) Poultry & Poultry Aerobic plate count Chapter 3, US FDA - BAM IS 5402 / ISO & 2 Escherichia coli Chapter 4, US FDA - BAM IS 5887 (P 1) Enumeration of Fecal Coliforms Chapter 4, US FDA - BAM Enumeration of Coliforms Chapter 4, US FDA - BAM IS 5401 (P 1) Staphylococcus aureus Chapter 12, US FDA - BAM IS 5887 (P 8 / Sec 1) IS 5887 (P 2) Salmonella IS 5887 (P 3) Chapter 5, US FDA - BAM , Chapter 17, AOAC Page 3 of 27

4 Microbiology Poultry & Poultry Yeast and Mold count Chapter 18, US FDA - BAM IS 5403 Listeria monocytogenes Chapter 10, US FDA - BAM IS (P 1) , Chapter 17, AOAC Clostridium perfringens ISO 7937 IS 5887 (P 4) Shigella spp IS 5887 (P 7) Vegetables & Vegetables Aerobic plate count Chapter 3, US FDA - BAM IS 5402 ISO & 2 Escherichia Coli Chapter 4, US FDA - BAM IS 5887 (P 1) ISO Enumeration of Fecal Coliforms Chapter 4, US FDA - BAM Enumeration of Coliforms Chapter 4, US FDA - BAM IS 5401 (P 1) Shigella spp IS 5887 (P 7) Staphylococcus aureus Chapter 12, US FDA - BAM IS 5887 (P 8 / Sec 1) IS 5887 (P 2) Salmonella spp IS 5887 (P 3) Chapter 5, US FDA - BAM , Chapter 17, AOAC Vibrio cholerae Chapter 9, US FDA - BAM IS 5887 (P 5) Listeria monocytogenes Chapter 10, US FDA - BAM IS (P 1) , Chapter 17, AOAC Bacillus cereus Chapter 14, US FDA - BAM IS 5887 (P 6) Yeast and Mold count Chapter 18, US FDA - BAM IS 5403 Clostridium perfringens Chapter 16, US FDA - BAM IS 5887 (P 4) Lactic acid bacteria ISO Flat sour spore formers Clause 25.6, Chapter 25, APHA 5 th Edition Other Specified Food Items - Raw, Cooked Foods and Ready to Eat Foods Aerobic plate count Chapter 3, US FDA - BAM IS 5402 ISO & 2 Escherichia coli Chapter 4, US FDA - BAM IS 5887 (P 1) Enumeration of Fecal Coliforms Chapter 4, US FDA - BAM Enumeration of Coliforms Chapter 4, US FDA - BAM IS 5401 (P 1) Shigella spp IS 5887 (P 7) Staphylococcus aureus Chapter 12, US FDA - BAM IS 5887 (P 8 / Sec 1) Page 4 of 27

5 Microbiology Other Specified Food Items - Raw, Cooked Foods and Ready to Salmonella spp IS 5887 (P 3) Chapter 5, US FDA - BAM , Chapter 17, AOAC Eat Foods Yeast and Mold count Chapter 18, US FDA - BAM IS 5403 Bacillus cereus Chapter 14, US FDA - BAM IS 5887 (P 6) Listeria monocytogenes Chapter 10, US FDA - BAM IS (P 1) , Chapter 17, AOAC Clostridium perfringens Chapter 16, US FDA - BAM IS 5887 (P 4) Vibrio cholerae Chapter 9, US FDA BAM IS 5887 (P 5) ISO Enterobacteriaceae ISO Drinking Water - Piped and Bore Well Packaged Natural Mineral Water and Packaged Drinking Water Coliforms Escherichia coli Escherichia coli and coliforms by membrane filtration technique 9221 B, 9221 C, 9221 E, APHA IS B, 9221 E, APHA IS 1622 IS ISO Enterococci IS ISO Pseudomonas aeruginosa ISO Clostridium perfringens Annex 3, EU 98/83/EC Colony 22 0 C ISO 6222 Colony 37 0 C ISO 6222 Escherichia coli IS 5887 (P 1) IS ISO Coliform IS 5401 (P 1) IS ISO Fecal Streptococci IS 5887 (P 2) IS ISO Staphylococcus aureus IS 5887 (P 2) Sulphite reducing anaerobes IS Pseudomonas aeruginosa IS Yeast and Mold Count IS 5403 Aerobic Microbial Count IS 22 deg C & 37 Deg C Salmonella IS 5887 (P 3) IS Shigella IS 5887 (P 7) Vibrio choleraee IS 5887 (P 5) Page 5 of 27

6 Microbiology Packaged Natural Vibrio parahemolyticus IS 5887 (P 5) Mineral Water and Packaged Drinking Water Water for Processed Standard Plate Count IS 1622 Food Industry Coliform IS 1622 Proteolytic & Lipolytic count Appendix A, IS 4251 Microbiology Beverages - (Alcoholic /Non - Alcoholic) Cereal, Pulses and Cereal Cereal, Pulses and Cereal Aerobic Plate Count Chapter 3, US FDA - BAM IS 5402 ISO Escherichia coli Chapter 4, US FDA - BAM ISO Yeast and Mold Count Chapter 18, US FDA - BAM IS 5403 ISO Salmonella spp IS 5887 (P 3) ISO , Chapter 17, AOAC Shigella spp IS 5887 (P 7) Staphylococcus aureus Chapter 12, US FDA - BAM IS 5887 (P 8 / Sec 1) ISO Enumeration of Coliforms Chapter 4, US FDA - BAM IS 5401 (P 1) IS 5401 (P 2) ISO 4831 ISO 4832 Enumeration of Fecal Coliforms Chapter 4, US FDA - BAM Vibrio cholerae IS 5887 (P 5) Flat Sour Spore Organism Clause 25.6, Chapter 25, APHA 5 th Edition Staphylococcus aureus Chapter 12, US FDA - BAM IS 5887 (P 2) IS 5887 (P 8 / Sec 1) Salmonella spp IS 5887 (P 3) Chapter 5, US FDA - BAM , Chapter 17, AOAC Yeast and Mold count Chapter 18, US FDA - BAM IS 5403 Bacillus cereus ISO 7932 Chapter 14, US FDA BAM Faecal Streptococci IS 5887 (P 2) Aerobic Plate Count Chapter 3, US FDA - BAM IS 5402 ISO & 2 Enumeration of Coliforms Chapter 4, US FDA - BAM IS 5401 (P 1) IS 5401 (P 2) ISO 4832 Page 6 of 27

7 Microbiology Coffee and Cocoa Escherichia coli Cereal, Pulses and Cereal Chapter 4, US FDA - BAM ISO IS 5887 (P 1) Aerobic Plate Count Chapter 3, US FDA - BAM IS 5402 ISO & 2 Escherichia coli Chapter 4, US FDA - BAM IS 5887 (P 1) Enumeration of Coliforms Chapter 4, US FDA - BAM IS 5401 (P 1) Enumeration of Fecal Coliforms Chapter 4, US FDA - BAM Staphylococcus aureus Chapter 12, US FDA - BAM IS 5887 (P 8 / Sec 1) Salmonella spp Chapter 5, US FDA - BAM IS 5887 (P 3) , Chapter 17, AOAC Yeast and Mold Count Chapter 18, US FDA - BAM IS 5403 Bacillus cereus Chapter 14, US FDA - BAM ISO 7932 Clostridium perfringens ISO 7937 Chapter 16, US FDA BAM Fruit and Fruit Total Plate Count Chapter 3, US FDA - BAM IS 5402 ISO & 2 Coliform Count Chapter 4, US FDA - BAM IS 5401 (P 1) IS 5401 (P 2) ISO 4832 ISO 4831 Escherichia coli Chapter 4, US FDA - BAM ISO IS 5887 (P 1) Shigella spp IS 5887 (P 7) Staphylococcus aureus Chapter 12, US FDA - BAM IS 5887 (P 8 / Sec 1) IS 5887 (P 2) Salmonella spp IS 5887 (P 3) Chapter 5, US FDA - BAM , Chapter 17, AOAC Vibrio cholerae IS 5887 (P 5) Chapter 9, US FDA - BAM Listeria monocytogenes Chapter 10, US FDA - BAM IS (P 1) , Chapter 17, AOAC Bacillus cereus Chapter 14, US FDA - BAM IS 5887 (P 6) Yeast and Mold Count Chapter 18, US FDA - BAM IS 5403 Page 7 of 27

8 Microbiology Fruit and Fruit Clostridium perfringens Chapter 16, US FDA - BAM ISO 7937 IS 5887 (P 4) Lactic acid bacteria ISO Incubation 37 deg for 10 IS 2860 days & 55 deg for 7 days Flat sour spore formers Clause 25.6, Chapter 25, APHA 5 th Edition Herbs, Spices and Condiments Milk and Dairy Aerobic Plate Count Chapter 3, US FDA - BAM IS 5402 ISO Escherichia coli Chapter 4, US FDA - BAM ISO IS 5887 (P 1) Enumeration of Coliforms Chapter 4, US FDA - BAM IS 5401 (P 1) ISO 4832 Enumeration of Fecal Coliforms Chapter 4, US FDA - BAM Salmonella spp Chapter 5, US FDA - BAM IS 5887 (P 3) , Chapter 17, AOAC ISO 6579 Yeast and Mold Count Chapter 18, US FDA - BAM IS 5403 ISO Bacillus cereus Chapter 14, US FDA - BAM IS 5887 (P 6) ISO 7932 Clostridium perfringens Chapter 16, US FDA - BAM ISO 7937 IS 5887 (P 4) Staphylococcus aureus Chapter 12, US FDA - BAM IS 5887 (P 8 / Sec 1) ISO Shigella spp IS 5887 (P 7) Sulfite reducing bacteria ISO Aerobic Plate Count Chapter 3, US FDA - BAM IS 5402 ISO Escherichia coli Chapter 4, US FDA - BAM ISO IS 5887 (P 1) Enumeration of Coliforms Chapter 4, US FDA - BAM IS 5401 (P 1) ISO 4832 Enumeration of Fecal Coliforms Chapter 4, US FDA - BAM Shigella spp IS 5887 (P 7) Page 8 of 27

9 Microbiology Milk and Dairy Staphylococcus aureus Chapter 12, US FDA - BAM IS 5887 (P 8 / Sec 1) IS 5887 (P 2) ISO Salmonella spp Chapter 5, US FDA - BAM IS 5887 (P 3) , Chapter 17, AOAC ISO 6579 Yeast and Mold Count Chapter 18, US FDA - BAM IS 5403 ISO Bacillus cereus Chapter 14, US FDA - BAM IS 5887 (P 6) ISO 7932 Listeria monocytogenes Chapter 10, US FDA - BAM IS (P 1) , Chapter 17, AOAC ISO Clostridium perfringens Chapter 16, US FDA - BAM ISO 7937 IS 5887 (P 4) Enterobacteriaceae ISO Tea Aerobic Plate Count Chapter 3, US FDA - BAM IS 5402 ISO Escherichia coli Chapter 4, US FDA - BAM ISO IS 5887 (P 1) Enumeration of Coliforms Chapter 4, US FDA - BAM IS 5401 (P 1) ISO 4832 Enumeration of Fecal Coliforms Chapter 4, US FDA - BAM Staphylococcus aureus Chapter 12, US FDA - BAM IS 5887 (P 8 / Sec 1) ISO Salmonella spp Chapter 5, US FDA - BAM IS 5887 (P 3) , Chapter 17, AOAC ISO 6579 Yeast and Mold Count Chapter 18, US FDA - BAM IS 5403 ISO ISO Bacillus cereus Chapter 14, US FDA - BAM ISO 7932 Listeria monocytogenes Chapter 10, US FDA - BAM , Chapter 17, AOAC ISO Page 9 of 27

10 Microbiology Tea Clostridium perfringens Chapter 16, US FDA - BAM ISO 7937 IS 5887 (P 4) Enterobacteriaceae ISO Wet Chemical Fish and Marine Total Solids / Moisture , Chapter 35, AOAC FSSAI Lab Manual 6 (Clause 2.2) Total Fat , Chapter 35, AOAC Total Nitrogen , Chapter 35, AOAC Total Ash , Chapter 35, AOAC Sodium Chloride , Chapter 35, AOAC FSSAI Lab Manual 6 (Clause 2.3) Total Volatile Base Nitrogen LAB_P_SOP_129 FSSAI Lab Manual 6 (Clause 1.2) Acid Insoluble Ash IS Annex F FSSAI Lab Manual 6 (Clause 2.4) Polyphosphates IS 5960 (P 13) Clause 8 ISO 5553 FSSAI Lab Manual 6 (Clause 3.5) Lead, Cadmium, Mercury & Lab_P_SOP_36 / AAS Arsenic Meat And Poultry Moisture , Chapter 39, AOAC Crude fat , Chapter 39, AOACFSSAI Lab Manual 6 (Clause 2.1) Salt , Chapter 39, AOAC FSSAI Lab Manual 6 (Clause 2.3) Total ash , Chapter 39, AOACFSSAI Lab Manual 6 (Clause 2.4) Nitrogen , Chapter 39, AOACFSSAI Lab Manual 6 (Clause 2.2) Drinking Water / Packaged Drinking Water / Mineral Water / Process Water (Council Directive 98/83/EC) Copper, Zinc, Tin, Mercury, Arsenic, Lead, Cadmium & Selenium LAB_P_SOP_50 AAS Colour IS 3025 (P 4) Clause B of APHA Total dissolved solids IS 3025 (P 16) 2540 C of APHA Odour IS 3025 (P 5) 2150 B of APHA Turbidity IS 3025 (P 10) 2130 B of APHA Taste IS 3025 (P 8) 2160 C of APHA Aluminium IS 3025 (P 55) Boron IS 3025 (P 57) Residual free chlorine IS 3025 (P 26) Clause Cl-B of APHA Page 10 of 27

11 Wet Chemical Drinking Water / Packaged Drinking Hexavalent chromium IS 3025 (P 52) Clause Cr B of APHA Water / Mineral Water / Process Nitrites IS 3025 (P 34) Clause NO 2 B of APHA Water (Council Directive 98/83/EC) ph value IS 3025 (P 11) Clause H+ B of APHA Total hardness (as CaCO 3 ) IS 3025 (P 21) Clause C of APHA Copper (as Cu) IS 3025 (P 42) Iron (as Fe) IS 3025 (P 53) Manganese (as Mn) IS 3025 (P 35) Nitrate (as NO 3 ) IS 3025 (P 34) Clause NO 3 B of APHA Flouride (as F) IS 3025 (P 60) Clause F - D of APHA Zinc (as Zn) IS 3025 (P 49) Nickel (as Ni) Annexure F of IS Chloride (as Cl) IS 3025 (P 32) Clause C of APHA Selenium (as Se) IS 3025 (P 56) Sulphate (as SO 4 ) IS 3025 (P 24) Clause SO 4 E of APHA Alkalinity (as CaCO 3) IS 3025 (P 23) 2320 B of APHA Total Solids IS 3025 (P 15) Calcium (as Ca) IS 3025 (P 40) Clause Ca B of APHA Phenolic compounds (as C 6 H 5 OH) IS 3025 (P 43) 5530 D of APHA Mercury (as Hg) IS 3025 (P 48) Cadmium (as Cd) IS 3025 (P 41) Arsenic (as As) IS 3025 (P 37) Lead (as Pb) IS 3025 (P 47) Chromium (as Cr) Annexure J of IS Barium (as Ba) Annexure F of IS Magnesium (as Mg) IS 3025 (P 46) 3500 Mg B of APHA Page 11 of 27

12 Wet Chemical Drinking Water/ Packaged Drinking Sodium (as Na) IS 3025 (P 45) Water/Mineral Water/Process Silver (as Ag) Annex J IS Water (Council Directive 98/83/EC) Molybdenum (as Mo) IS 3025 (P 2) Mineral Oil IS 3025 (P 39) 5520 C of APHA Anionic Surface Active Agents as MBAS Annex K of IS C of APHA Sulphide as H 2 S IS 3025 (P 29) Clause S 2 - F of APHA Antimony (as Sb) Annex G IS Cyanide (as CN) IS 3025 (P 27) Clause CN - E of APHA Borates (as B) Annex H of IS Chloramines as Cl Cl -G APHA Ammonia (as Total Ammonia N) IS 3025 (P 34) Clause NH 3 C of APHA Oxidisability IS 3025 (P 58) Dissolved oxygen IS 3025 (P 38) Clause 4 Total Organic Carbon (TOC) In-house method Specific conductance IS 3025 (P 14) 2510 B of APHA Milk (Raw and Pasteurized)/Curd/ Lassi/Condensed Milk/Sweetened UHT Treated Condensed Milk Total Milk Solids & Total Solids Titratable Acidity as Lactic Acid ph IS 1166 Append B IS 1479 Clause 4 IS IS Clause , Chapter 33, AOAC FSSAI Lab Manual 1 (Clause 1.3.3) IS 1166 Append D IS Clause 8 IS 1479 (P 1) Clause , Chapter 33, AOAC FSSAI Lab Manual 1 (Clause 9.5) IS 4238 Append C Page 12 of 27

13 Wet Chemical Milk (Raw and Pasteurized)/Curd/ Lassi/Condensed Milk/Sweetened UHT Treated Condensed Milk Total Fat IS Clause 5B IS 1479 (P 2) IS IS 1166 FSSAI Lab Manual 1 (Clause 1.3.4) Total Ash IS 1479 (P 2) Clause , Chapter 33, AOAC FSSAI Lab Manual 1 (Clause 10.7) Lactose IS 1479 (P 2) Clause 13 Sucrose IS 1166 Append C FSSAI Lab Manual 1 (Clause 9.4) Crude Protein IS 1479 (P 2) Clause 8 IS 7219 FSSAI Lab Manual 1 (Clause 19.0) Total Nitrogen IS 1479 (P 2) Clause ,Chapter 33, AOAC FSSAI Lab Manual 1 (Clause 19.0) Solids-Not-Fat (SNF) ,Chapter 33, AOAC FSSAI Lab Manual 1 (Clause 15.2) Titratable Acidity (as Acetic Acid) IS Append D FSSAI Lab Manual 1 (Clause 9.5) Total Antibiotics as Beta Lactum Lab_P_SOP_238(Detection of Antibiotic Residue in Milk and Milk Power) / Delvo Kit Dry Milk Powder (Milk Powder, Skim Milk Powder, Partly Skimmed Milk Powder, Infant Milk Substitute) Moisture IS IS IS FSSAI Lab Manual 1 (Clause 10.2) Total Solids IS 1165 IS (P 1) IS (P 2) IS FSSAI Lab Manual 1 (Clause 10.2) Sucrose IS 4079 Append C FSSAI Lab Manual 1 (Clause 9.4) Fat IS IS FSSAI Lab Manual 1 (Clause 10.3) Total Ash IS 1165 Append A , Chapter 33, AOAC FSSAI Lab Manual 1 (Clause 10.7) Page 13 of 27

14 Wet Chemical Dry Milk Powder (Milk Powder, Skim Milk Powder, Partly Skimmed Milk Powder, Infant Milk Substitute) Titratable Acidity as Lactic Acid Cheese (Includes Natural & Processed Cheese) & Paneer IS 1165 Append B IS FSSAI Lab Manual 1 (Clause 10.4) Protein / Milk Protein in MSNF IS , Chapter 33, AOAC FSSAI Lab Manual 1 (Clause Total Antibiotics as Beta Lactum Moisture Milk Fat 10.6) Lab_P_SOP_238 (Detection of Antibiotic Residue in Milk and Milk Power)/ Delvo Kit IS 2785 Append A IS Append A , Chapter 33, AOAC IS Append B Salt IS 2785 Append C Dairy Milk and Milk Animal Feed / Pet Foods Titratable Acidity as Lactic Acid Ash Total Nitrogen ph Copper, Zinc, Tin, Mercury, Arsenic, Lead, Cadmium & Selenium Aflatoxin M1 IS Append C , Chapter 33, AOAC19th Edition , Chapter 33, AOAC , Chapter 33, AOAC , Chapter 33, AOAC Lab_P_SOP_36 / AAS LAB_P_SOP_235 LC-MS/MS Moisture IS 7874 (P 1) , Chapter 4, AOAC Clause 4 Crude Fat IS 7874 (P 1) , Chapter 4, AOAC Clause 7 Protien IS 7874 (P 1) , Chapter 4, AOAC Clause 5 Ash IS 7874 (P 1) , Chapter 4, AOAC Clause 9 Sugars , Chapter 4, AOAC Crude Fibre IS 7874 (P 1) Clause 8 Animal Feeds Lead, Cadmium, Arsenic & Lab_P_SOP_36 / AAS Mercury Wheat Atta Moisture IS 1155 Append A FSSAI Lab Manual 3 (Clause 8.2) Total Ash IS 1155 Append B FSSAI Lab Manual 3 (Clause 8.3) Page 14 of 27

15 Wet Chemical Wheat Atta Gluten IS 1155 Append D FSSAI Lab Manual 3 (Clause 8.5) Acid Insoluble Ash IS 1155 Append C FSSAI Lab Manual 3 (Clause 8.4) Alcoholic Acidity IS 1155 Append F FSSAI Lab Manual 3 (Clause 8.6) Crude Fiber IS 1155 Append E FSSAI Lab Manual 3 (Clause 8.9) Maida Gluten IS 1009 Append D FSSAI Lab Manual 3 (Clause 8.5) Moisture IS 1009 Append A FSSAI Lab Manual 3 (Clause 8.2) Ash IS 1009 Append B FSSAI Lab Manual 3 (Clause 8.3) Acid insoluble ash IS 1009 Append C FSSAI Lab Manual 3 (Clause 8.4) Alcoholic Acidity IS 1009 Append E FSSAI Lab Manual 3 (Clause 8.6) Cereals, Pulses and By Copper, Zinc, Tin, Mercury, Arsenic, Lead & Cadmium Lab_P_SOP_36 / AAS Spices and Condiments Pepper - Black, Kalimirchi White (Whole, Powder) Turmeric Haldi (Whole, Powder) Moisture IS 1797 Clause 9 3.0) / IS 1798 Total ash IS 1797 Clause 6 4.0) / IS 1798 Non-volatile ether extract IS 1797 Clause ) / IS 1798 Volatile oil IS 1797 Clause ) / IS 1798 Extraneous matter IS 1797 Clause 4 2.0) / IS 1798 Light Berries IS 1797 Clause ) / IS 1798 Pinheads or broken berries IS 1798 Clause 4 Bulk density Lab_P_SOP_16 (Determination of Bulk Density) 13.1) Piperine , Chapter 43, AOAC 13.3) Moisture IS 1797 Clause 9 3.0) Page 15 of 27

16 Wet Chemical Turmeric - Haldi (Whole, Powder) Total Ash IS 1797 Clause 9 4.0) Acid Insoluble Ash IS 1797 Clause 9 5.0) Cumin- Safed Zeera (Whole, Powder) Cumin Black Kalonji (Whole, Powder) Cumin Black Kalonji (Whole, Powder) Colouring power expressed as curcuminoid content IS 3576 / IS Append B 15.4) Total Starch IS 4706 Clause ) Test for Lead Chromate IS 3576 Annex A 15.6) Extraneous Matter IS 3576 Clause ) Defective rhizomes IS 3576 Clause 4.4 Moisture IS 1797 Clause 9 3.0) Total Ash IS 1797 Clause 6 4.0) Acid Insoluble Ash IS 1797 Clause 8 5.0) Volatile oil Content IS 1797 Clause ) Non Volatile Ether Extract IS 1797 Clause ) Extraneous Matter IS 1797 Clause 4 2.0) Broken Fruits (Dam Discoloured IS 2447 Clause 4 and Immature Seeds) Moisture IS 1797 Clause 9 3.0) Total Ash IS 1797 Clause 6 4.0) Acid Insoluble Ash IS 1797 Clause 8 5.0) Page 16 of 27

17 Wet Chemical Cumin Black Kalonji (Whole, Powder) Volatile Oil Content IS 1797 Clause ) Non Volatile Ether Extract IS 1797 Clause ) Extraneous Matter IS 1797 Clause 4 2.0) Broken Fruits (Damaged, IS 1797 Clause 4 Shrivelled, Discoloured and Immature Seeds) Edible seeds other than Cumin Black IS 2447 Clause 4 Cardamom - Choti Elachi, Large Cardamom - Badi Elachi (Whole, Seeds, Powder) Chillies and Capsicum (Whole, Powder) Moisture IS 1797 Clause 9 3.0) Total Ash IS 1797 Clause 6 4.0) Volatile Oil Content IS 1797 Clause ) Extraneous Matter IS 1797 Clause 4 2.0) Moisture IS 1797 Clause 9 3.0) Total ash IS 1797 Clause 6 4.0) Acid insoluble ash IS 1797 Clause 8 5.0) Volatile oil content IS 1797 Clause ) Extraneous matter IS 2322 Clause ) Unripe and marked fruits IS 2322 Clause 5.5 Broken fruits, seed & fragments IS 2322 Clause 5.5 Crude fiber IS 1797 Clause ) Non-volatile ether extract IS 1797 Clause ) Page 17 of 27

18 Wet Chemical Chillies and Capsicum (Whole, Powder) Colour , Chapter 43, AOAC / ASTA Colour Cinnamon Dalchini (Whole, Powder) Cloves Laung (Whole, Powder) Coriander Dhania (Whole, Powder) Moisture IS 1797 Clause 9 3.0) Total Ash IS 1797 Clause 6 4.0) Acid Insoluble Ash IS 1797 Clause ) Volatile Oil Content IS 1797 Clause ) Extraneous Matter IS 1797 Clause 4 2.0) Moisture IS 1797 Clause 9 3.0) Total ash IS 1797 Clause 6 4.0) Acid insoluble ash IS 1797 Clause ) Volatile oil IS 1797 Clause ) Crude fiber IS 1797 Clause ) Extraneous matter IS ) Tendrils, Mother cloves IS 4404 Khokar cloves IS 4404 Headless cloves IS 4404 Moisture IS 1797 Clause 9 3.0) Total ash IS 1797 Clause 6 4.0) Acid insoluble ash IS 1797 Clause ) Page 18 of 27

19 Wet Chemical Coriander Dhania (Whole, Powder) Volatile oil content IS 1797 Clause ) Extraneous matter IS 1797 Clause 4 2.0) Split fruits IS 1797 Clause 4 Damaged / discolored fruits IS 1797 Clause 4.1 Spices and Condiments Moisture IS: ) Total ash IS: ) Acid insoluble ash IS: Non-volatile ether extract IS: ) Volatile oil IS: ) Extraneous matter IS: ) Copper, Zinc, Tin, Mercury, Arsenic, Lead & Cadmium Lab_P_SOP_36 / AAS Wet Chemical Fruit and Vegetable (Canned Fruits and Vegetables) Fruit and Vegetable (Pickles) Total soluble solids IS , Chapter 37, AOAC FSSAI Lab Manual 5 (Clause 1.6) ph IS 2860 Clause 8 / IS 3881 Annex D FSSAI Lab Manual 5 (Clause 2.3) Protein IS , Chapter 37, AOAC Salt IS 2860 Clause 11/ IS 1797 Clause 16 Reducing Sugars IS 6287 Clause 8 FSSAI Lab Manual 5 (Clause 2.6) Sucrose IS 6287 Clause 9 FSSAI Lab Manual 5 (Clause 2.6) Titratable Acidity IS 2860 Clause , Chapter 37, AOAC Drained weight / net weight IS 2860 Clause 7 Vitamin C Chapter 45, AOAC ph IS 2860 Clause 8 / IS 3881 Annex D Protein IS , Chapter 37, AOAC Page 19 of 27

20 Wet Chemical Fruit and Vegetable Salt IS 2860 / IS 1797 Clause 16 (Pickles) Reducing Sugars IS 6287 Clause 8 Sucrose IS 6287 Clause 9 Titratable Acidity IS , Chapter 37, AOAC FSSAI Lab Manual 5 (Clause 2.4) Drained weight / Net weight IS 2860 FSSAI Lab Manual 5 (Clause 1.4) Vitamin C , Chapter 45, AOAC Sulphur di oxide , Chapter 47, AOAC Fruit and Vegetable (Jam, Jellies, Marmalades) Fruit and Vegetable (Dehydrated and Frozen Fruits and Vegetables) Total soluble solids IS , Chapter 37, AOAC FSSAI Lab Manual 5 (Clause 17.3) ph IS 2860 Clause 8 / IS 3881 Annex D Protein IS , Chapter 37, AOAC Reducing Sugars IS 6287 Clause 8 Sucrose IS 6287 Clause 9 Titratable Acidity IS , Chapter 37, AOAC FSSAI Lab Manual 5 (Clause 2.4) Vitamin C , Chapter 45, AOAC Sulphur di oxide , Chapter 47, AOAC Water Insoluble solids , Chapter 37, AOAC Soluble solids , Chapter 37, AOAC Total Ash , Chapter 37, AOAC ph IS 2860 Clause 8 / IS 3881 Annex D FSSAI Lab Manual 5 (Clause 17.3) Protein IS , Chapter 37, AOAC Salt IS 2860 / IS 1797 Reducing Sugars IS 6287 Clause 8 Sucrose IS 6287 Clause 9 Titratable Acidity IS , Chapter 37, AOAC Vitamin C , Chapter 45, AOAC Water Insoluble solids , Chapter 37, AOAC Soluble solids , Chapter 37, AOAC Total Ash , Chapter 37, AOAC Moisture Lab_P_SOP_247 FSSAI Lab Manual 5 (Clause 4.1) Acid Insoluble Ash Lab_P_SOP_248 FSSAI Lab Manual 5 (Clause 17.4) Page 20 of 27

21 Wet Chemical Fruit and Vegetable Starch IS 4706 (P 2) Clause 9 (Dehydrated and Frozen Fruits and Vegetables) Sulphur di oxide , Chapter 47, AOAC Fruit and Vegetable (Ketchup, Sauces, Culinary Paste) Total soluble solids IS , Chapter 37, AOAC FSSAI Lab Manual 5 (Clause 1.6) ph IS 2860 / IS 3881 Annex D Protein IS , Chapter 37, AOAC Salt IS 2860 / IS 1797 Clause 16 Reducing Sugars IS 6287 Clause 8 Sucrose IS 6287 Clause 9 Titratable Acidity IS , Chapter 37, AOAC FSSAI Lab Manual 5 (Clause 2.4) Vitamin C , Chapter 45, AOAC Fill of container Lab_P_SOP_250 (Determination of percentage fill of container in fruit and vegetable products) Fruit and Vegetable (Fruit Juice / Beverages, Squash, Cordial, Crush, Carbonated Juices) Alcoholic and Non - Alcoholic Beverages (including Carbonated Beverages) Acid Insoluble Ash Lab_P_SOP_248 Determination of Ash Insoluble in Ash in Fruits and Vegetable product Total soluble solids IS , Chapter 37, AOAC FSSAI Lab Manual 5 (Clause 1.6) Fruit content Lab_P_SOP_244 FSSAI Lab Manual 5 (Clause 2.11) ph IS 2860 / IS 3881 Annex D FSSAI Lab Manual 5 (Clause 2.3) Reducing Sugars IS 6287 Clause 8 FSSAI Lab Manual 5 (Clause 2.6) Sucrose IS 6287 Clause 9 FSSAI Lab Manual 5 (Clause 2.6) Titratable Acidity , Chapter 37, AOAC / FSSAI Lab Manual 5 (Clause 2.4) Sulphur di oxide , Chapter 47, AOAC Copper, Zinc, Tin, Mercury, Lab_P_SOP_36 / AAS Arsenic, Lead & Cadmium Page 21 of 27

22 Wet Chemical Coffee (Roasted Coffee/R&G Coffee/Soluble Coffee Powder, Total ash IS , Chapter 30, AOAC Annex F FSSAI Lab Manual 4 (Clause 1.3) Soluble Coffee - Chicory Powder, Acid insoluble ash IS 3077 Annex G FSSAI Lab Manual 4 (Clause 1.5) Roasted Coffee - Chicory Powder, Water soluble ash IS 3077 Annex H FSSAI Lab Manual 4 (Clause 1.4) Instant Coffee - Chicory Mixture) Alkalinity of ash IS 3077 Annex J FSSAI Lab Manual 4 (Clause 1.6) Ether Extract IS 3077 Annex M Chapter 30, AOAC Aqueous extract IS 3077 Annex K FSSAI Lab Manual 4 (Clause 1.7) Moisture IS 3077 Annex E , Chapter 30, AOAC FSSAI Lab Manual 4 (Clause 1.2) Acidity , Chapter 30, AOAC Crude fiber , Chapter 30, AOAC Total ash IS 612 FSSAI Lab Manual 4 (Clause 1.3) Tea (Black Tea, Green Tea, Instant Tea) Coffee, Tea, Cocoa and By Total ash IS / IS FSSAI Lab Manual 4 (Clause 1.3) Acid insoluble ash IS FSSAI Lab Manual 4 (Clause 1.5) Water soluble ash IS FSSAI Lab Manual 4 (Clause 1.4) Alkalinity of ash IS FSSAI Lab Manual 4 (Clause 1.6) Moisture IS FSSAI Lab Manual 4 (Clause 1.2) Water soluble Extract IS FSSAI Lab Manual 4 (Clause 1.7) Total polyphenols as Gallic Acid ISO Crude fiber IS (P 1) FSSAI Lab Manual 4 (Clause 5.8) Total ash , Chapter 30, AOAC FSSAI Lab Manual 4 (Clause 1.3) Acid insoluble ash IS 3633 Alkalinity of ash IS 3633 Copper, Zinc, Tin, Mercury, Lab_P_SOP_36 / AAS Arsenic, Lead, Cadmium, Selenium, Manganese, Chromium, Iron & Nickel Page 22 of 27

23 Chemical (Analytical) Food & Agricultural Commodities Pesticide Residues: 4-Bromo-2-Chlorophenol, Alachlor, Aldrin, Allethrins, Bendiocarb, Bifenthrin, Bioallethrin, Butachlor, Carbaryl, Captafol, Captan, Chlordane-cis & trans, Chlorfenopyr, Chlorfenvinphos, Chlorobenzilate, Chlorothalonil, Chlorpyrifosmethyl, ethyl, Cyfluthrin- I,II,III,IV, Cypermethrin - I, II, III, IV, Deltamethrin, Dichlorvos, Dicofol, Dieldrin, α-endosulfan, β- Endosulfan, Endosulfan - Sulphate, Endrin, Ethion, Ethofenprox, Etrimfos,Fenitrothion, Fenpropathrin, Fenvalerate-I,II, Esfenvalerate, Fluvalinate-I,II, Fluchloralin, Formothion, HCB, Heptachlor, Heptachlor epoxide, Imazethapyr,Iprodione, Isodrin, Methoxychlor, Metolachlor, Mirex, o,p', p,p' DDD, o,p', p,p' - DDE, o,p', p,p' DDT, Oxyfluorfen, Parathion-ethyl, methyl, Pendimethalin, Permethrin-I,II, Phorate, Pretilachlor, Propanil, Propetamphos, Pyrene, Pyrethrins, Tau-Fluvalinate, Tetraconazole, Tetrahydrophthalimide, Transfluthrin, Trifluralin, Vinconazolin, α-bhc, β-bhc, ϒ- BHC, δ-bhc, λ-cyhalothrin AOAC 20 th Edition 2015 EN : 2009 LAB_P_SOP_50-Fish & Fish LAB_P_SOP_97-Dairy & Dairy LAB_P_SOP_137- Beverages LAB_P_SOP_202- Cereals, Pulses & their LAB_P_SOP_207-Spices Condiments & their products LAB_P_SOP_260 Fruits & Vegetables LAB_P_SOP_287 Simulant Foods By GC-MS/MS Lab_P_SOP_198 Fish & Fish GCMSMS Page 23 of 27

24 Chemical (Analytical) Food & Agricultural Commodities Pesticide Residues: 2,4 - D, 3,4-Dichloroaniline, 3- Hydroxy Carbofuran, Acephate, Acetamiprid, Aldicarb, Aldicarb sulfone, Aldicarb Sulfoxide, Anilophos, Atrazine, Benfuracarb, Benomyl, Bitertanol, Buprofezin, Carbaryl, Carbendazim, Carbofuran, Carbosulfan, Carpropamid, Cartaphydrochloride, Chlorimuron-ethyl, Chlormequatchloride, Clomazone, Clodinafop-propargyl, Cyhalofopbutyl, Cymoxanil, Diazinon, Diclofop-methyl, Difenoconazole, Diflubenzuron, Dimethoate, Dimethomorph, Dithianon, Diuron, Dodine, Edifenphos, Ethephon, Ethion, Ethoxyquin, Ethoxysulfuron, Fenamidone, Fenarimol, Fenazaquin, Fenobucarb, Fenoxy-prop-p-ethyl, Fenthion, Fenthion sulfone, Fenthion sulfoxide, Formothion, Flonicamid, Flufenacet, Fosetyl-Al, Glufosinate-ammonium, Glyphosphate, Hexaconazole, Imidacloprid, Indoxacarb, Iprodione, Isoprothiolane, Isoproturon, Kitazin (Iprobenphos), Linuron, Lufenuron, Malaoxon, Malathion, Malic Hydrazide, Metalaxyl, Metalaxyl-M, Metasulfuronmethyl, Methabenzthiazuron, Methamidophos, Methomyl, Methyl Chloro-phenoxy-acetic Acid (M.C.P.A.), Metribuzin, Monocrotophos, Myclobutanil, Novaluron, O-methoate, Oxadiargyl, Oxadiazon, Oxydemeton methyl, Paclobutrazole, Paraoxon, Paraoxon-methyl, Paraquat, Penconazole, Phenthoate, Phorate sulfone, Phorate sulfoxide, Phosalone, Phosphamidon, Pirimiphos-methyl, Profenofos, AOAC 20 th Edition 2015 EN : 2009 LAB_P_SOP_50-Fish & Fish LAB_P_SOP_97-Dairy & Dairy LAB_P_SOP_137- Beverages LAB_P_SOP_202- Cereals, Pulses & their LAB_P_SOP_207-Spices Condiments & their products LAB_P_SOP_260 Fruits & Vegetables LAB_P_SOP_287 Simulant Foods By GC-MS/MS Lab_P_SOP_198 Fish & Fish LCMSMS Page 24 of 27

25 Chemical (Analytical) Food & Agricultural Commodities Propargite, Propiconazole, Pyrazosulfuron ethyl, Quinolphos, Quizalofop-ethyl, Simazine, Spinosad-A, D, Sulfosulfuron, Tebuconazole, Thiamethoxam, Thiochlorprid, Thiodicarb, Thiometon, Thiophenatemethyl, TNFA, TNFG, Triadimefon, Triadimenol, Triallate, Triazophos, Trichlorfon, Tricyclazole, Tridemorph, α-naphthyl acetic acid Food and Agricultural Commodities Food and Agricultural, Animal Feeds Food and Agricultural Commodities Nut and Nut, Cereals & Grains, Coffee Fish And Marine Pesticide Residues: Dithiocarbamates as CS 2, Mancozeb, Metiram, & Propineb Aflatoxins B1, B2, G1 & G2 Copper Oxychloride Aflatoxin B1, B2, G1 & G2 Chloramphenicol Sulfonamides: Sulfamethoxazole, Sulfadoxine, Sulfamerazine, Sulfadiazine, Sulfadimethoxine, Sulfapyridine, Sulfamethazine, Sulfachloropyridazine, Sulfamethoxypyridazine, Sulfamethizole, Sulfathiazole Nitrofuran Metabolties: AHD, AMOZ, AOZ, SEM Nitrofurans: Furaltadone, Furazolidone, Nitrofurantoin, Nitrofurazone Dyes: Crystal violet, Leuco Crystal Violet, Malachite green, Leuco Malachite Green Tetracyclines & Epimers: Chlorotetracycline, 4-epi- Chlortetracycline, Oxyteracycline, 4-epi-Oxytetracycline, Tetracycline, 4-epi-Tetracycline LAB_P_SOP_210 GC-MS Lab_P_SOP_225 LC-MS/MS By AAS Lab_P_SOP_225 LC-MS/MS LAB_P_SOP_43 LC-MS/MS LAB_P_SOP_48 LC-MS/MS LAB_P_SOP_44 LC-MS/MS LAB_P_SOP_96 LC-MS/MS LAB_P_SOP_148 LC-MS/MS LAB_P_SOP_49 LC-MS/MS Page 25 of 27

26 Chemical (Analytical) Fish And Marine Fluoroquinolones: Ciprofloxacin, Danofloxacin, LAB_P_SOP_111 LC-MS/MS Difloxacin, Enrofloxacin, Flumequine, Oxolinic Acid, Sarafloxacin, Norfloxacin, Nalidixic acid Antihelmintics : Emamectin benzoate, Ivermectin LAB_P_SOP_315 LC-MS/MS Water (IS 14543:2004, IS 10500:1994, IS 13428:2005) Pesticide Residues: Aldrin, Carbaryl, Chlordane, Chlordane, Dieldrin, Endosulfanα, Endosulfan-β, Endosulfasulfate, Endrin,, Heptachlor, Heptachlorepoxide, Hexachlorobenzene, Lindane, Methoxychlor, o,p, p,p -DDD, o,p, p,p -DDE, o,p, p,p --DDT Pendimethalin, Quinolphos, Trifluralin, α HCH, β HCH, γ-hch, δ- HCH Polychlorobiphenyls: PCB28, PCB52, PCB101, PCB138, PCB153, PCB180 Ethoxyquin Pesticide Residues: Alachor, Aldrin,, Butachlor, Methyl parathion, Chlordane, Chlorpyrifos-methyl, Diledrin, o, p,p,p --DDT, o,p,p,p --DDE, o,p, p,p -DDD, Endosulfan-α, Endosulfan-β, Endosulfan sulfate, Ethion, Endrin, Heptachlor, Heptachlor epoxide, α-hch, β- HCH, γ-hch, δ-hch, Hexachlorobenzene, Parathionmethyl, ethyl, Phorate 2,4 D, Atrazine, Carbaryl, Ethion, Isoproturon, Malaoxon, Malathion, Methyl paraoxon, Monocrotophos, Pendimethalin, Phorate Sulfone, Phorate Sulfoxide, Quinolphos, Trifluralin & Simazine Polychlorobiphenyls (PCBs): PCB28, PCB52, PCB101, PCB138, PCB153, PCB180 LAB_P_SOP_50 GC-MS/MS LAB_P_SOP_50 GC-MS/MS LAB_P_SOP_198 LCMSMS LAB_P_SOP_46 GC-MS/MS LAB_P_SOP_46 LC-MS/MS LAB_P_SOP_46 GC-MS/MS Page 26 of 27

27 Chemical (Analytical) Water (IS 14543:2004, IS Polyaromatic Hydrocarbons (PAHs) LAB_P_SOP_46 GC-MS/MS 10500:1994, IS Acenaphthalene, Acenaphthene, 13428:2005) Anthracene, Benz(a)anthracene, (Council Directive 98/83/EC) Benzo(a)pyrene, Benzo(b)fluoranthene, Benzo(g,h,i)perylene, Benzo(k)fluoranthene, Chrysene, Di-benz(a,h)anthracene, Fluoranthrene, Fluorene, Indeno 1,2,3(c,d)pyrene, Naphthalene, Phenanthrene & Pyrene Water (Council Directive 98/83/EC) (IS 14543:2004, IS 10500:1994, IS 13428:2005) Volatile Organic Compounds (VOCs): 1,2-dichloroethane, Acrylamide, Benzene, Trihalomethanes (Bromodichloromethane, Bromoform, Chloroform, Dibromochloromethane), Epichlorohydrin, Tetrachloroethene, Trichloroethene & Vinyl Chloride LAB_P_SOP_45 GC-MS/MS LC-MS/MS Page 27 of 27