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1 Annex to declaration accreditation (scope accreditation) Location(s) where activities are performed under accreditation Head Office Jan van Galenstraat JG Schiedam The Netherlands Location Abbreviation/ location code Head fice Jan van Galenstraat JG Schiedam The Netherlands Jan van Galenstraat JG Schiedam The Netherlands Type activity 1 Sampling a Tap water Sampling water for Legionella analysis NL/M16 in accordance with NEN 6265 and ISO This annex has been approved by the Board the Dutch Accreditation Council, on its behalf, J.A.W.M. de Haas Director Operations 1 If there is a referral to a code starting with NAW, NAP, EA or IAF, this concerns a scheme mentioned on the RvA-BR010-lijst. If no date or version number is mentioned for a normative document, the accreditation concerns the most current version the document or scheme. Dutch Accreditation Council RvA Page 1 9
2 Annex to declaration accreditation (scope accreditation) Type activity 1 b Dried figs, groundnuts (peanuts), other oilseeds, nuts, driedfruits and spices Sampling for the analysis on mycotoxins NL/29 in accordance with Commission Regulation (EU) nr. 401/2006 Organic analyses 1 Vegetable and Determination the content Volatile Organic Contaminants; Headspace GC- MS NL/15 equivalent to ISO Food plant origin and animal origin (with low fat content) and their raw Determination the content dithiocarbamates (as CS2); Headspace GC-MS NL/10e (extraction ) in accordance with EN (analyse) equivalent to EN Food plant origin and animal origin and their raw and feed and their raw, animal oils, fats and fatty acids Determination the content dithiocarbamates (as CS2); Headspace GC-MS NL/10e (extraction ) (analyse) equivalent to EN Vegetable and animal oils and fats (with the exception olive oil) Determination the content the total amount and individual sterols; GC-FID cholesterol, brassicasterol, campesterol, stigmasterol, sitosterol, 5-avenasterol, 7-stigmasterol and 7-avenasterol NL/45 equivalent to ISO / 5 Food and feed plant origin and animal origin and Determination the content highly polar pesticides; LC-MS/MS NL/10d in accordance with QuPPe and QuPPe- AO Diquat, Paraquat, Glyfosate, AMPA, N-acetylglufosinate, Glufosinate, MPPA,Glufosinate sum, Phosphonic acid, Fosetyl, Fosetyl sum, Chlorate, Ethephon, Perchlorate Dutch Accreditation Council RvA Page 2 9
3 Annex to declaration accreditation (scope accreditation) Type activity 1 6 Feed and their raw, animal oils, fats and fatty acids Determination the content highly polar pesticides; LC-MS/MS Diquat, Paraquat, Glyfosate, AMPA, N-acetylglufosinate, Glufosinate, MPPA,Glufosinate sum, Phosphonic acid, Fosetyl, Fosetyl sum, Chlorate, Ethephon, Perchlorate NL/10d (sample preparation) in accordance with QuPPe and QuPPe- AO 7 Food and feed (with the exception spices) and their raw Determination the content Mycotoxins; LC-MS-MS Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2, Aflatoxin total, Ochratoxin A (OTA), Zearalenon (ZEA), Deoxynivalenol(DON/ Vomitoxin), HT2 Toxin, T2 Toxin, Fumonisin B1, Fumonisin B2 NL/13 8 Spices (with the exception pepper) Determination the content Mycotoxins; LC-MS-MS NL/13 Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2, Aflatoxin total, Ochratoxin A (OTA), Deoxynivalenol(DON/ Vomitoxin), Fumonisin B1, Fumonisin B2 9 Pepper Determination the content Mycotoxins; LC-MS-MS NL/13 Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2, Aflatoxin total, Deoxynivalenol (DON/ Vomitoxin), Fumonisin B1, Fumonisin B2 10 Spices Determination the content illegal dyes; LC-MS-MS NL/12 Sudan I, Sudan II, Sudan III, Sudan VI, Sudan red 7B, Sudan Red G, Sudan Orange G, Rhodamine B, Methanil Yellow, Dimethyl Yellow, Auramine O Basic Red 46, Chrysoidine G, Fast Garnet GBC, Sudan Black B, Sudan Red B, Toluidine Red, Orange II, Orange SS, Para Red, Bixin, Norbixin, Orange III, P-nitroaniline 11 Vegetable and Determination fatty-acid composition: GC-FID NL/16 in accordance with ISO / ISO Dutch Accreditation Council RvA Page 3 9
4 Annex to declaration accreditation (scope accreditation) Type activity 1 12 Vegetable and Determination the content mineral oil (fraction C10-C40): GC-FID NL/17 equivalent to VVR-II-OSP 15 and ISO Determination the content hydrocarbons (fraction C10-C56, C10- C25, C25-C56); GC-FID NL/17 14 Vegetable and animal oils and fats Determination MOSH and MOAH; online HPLC-GC-FID NL/46 MOSH/MOAH in accordance with CEN/TC 275 N 1069 MOSH equivalent to ISO and VVR-II- OSP15 15 Vegetable and animal fatty acids Determination MOSH and MOAH; online HPLC-GC-FID NL/46 MOSH/MOAH MOSH equivalent to ISO and VVR-II- OSP15 16 Food and feed and Determination the content WHO dioxins, dibenzurans and WHO dioxinlike PCB s and following non dioxin-like PCBs; GC-HR/MS NL/22a (sample preparation) in accordance with NEN-EN (feed) PCB 28, PCB 52, PCB 101, PCB 118, PCB 153, PCB 138 and PCB 180 (food) NL/22b in accordance with Directive (EU) nr. 2017/771 (feed and ) in accordance with Directive (EU) nr. 2017/644 (food and ) (non dioxin-like PCBs) Dutch Accreditation Council RvA Page 4 9
5 Annex to declaration accreditation (scope accreditation) Type activity 1 17 Food and feed and Determination the content Polycyclic AromaticHydrocarbons (PAH s); DACC- HPLC-fluorescence benzo(a)anthracene, chrysene, benzo(a)pyrene, NL/03 (sample preparation) in accordance with CEN/TS benzo(b)fluoranthene and the sum these 4 PAH s Phenanthrene, acenaphthene, anthracene, fluoranthene, pyrene, benzo(k)fluoranthene, dibenzo(ah)anthracene, benzo(ghi)perylene, indeno(1,2,3, c,d) pyrene, benzo[c]fluorene, 5- methylchrysene, benzo[j]fluoranthene, dibenzo[a,l]pyrene, dibenzo[a,e]pyrene, dibenzo[a,i]pyrene, dibenzo[a,h]pyrene in accordance with ISO Vegetable and Determination the content Polycyclic Aromatic Hydrocarbons (PAH s); DACC- HPLC-fluorescence benzo(a)anthracene, chrysene, benzo(a)pyrene, NL/03 in accordance with ISO benzo(b)fluoranthene and the sum these 4 PAH s Phenanthrene, acenaphthene, anthracene, fluoranthene, pyrene, benzo(k)fluoranthene, dibenzo(ah)anthracene, benzo(ghi)perylene, indeno(1,2,3, c,d) pyrene, benzo[c]fluorene, 5- methylchrysene, benzo[j]fluoranthene, dibenzo[a,l]pyrene, dibenzo[a,e]pyrene, dibenzo[a,i]pyrene, dibenzo[a,h]pyrene 19 Food and their raw Quantitative determination gluten (gliadin x2); ELISA NL/A001, NL/A002 and NL/A003 In accordance with AOAC-method Physical/ Chemical analyses 20 Grains and their s Determination moisture content; gravimetric NL/34a in accordance with ISO Feed and their raw Determination moisture content; gravimetric NL/34b and NL/34c in accordance with ISO 6496 Dutch Accreditation Council RvA Page 5 9
6 Annex to declaration accreditation (scope accreditation) Type activity 1 22 Feed and their raw, oleaginous seeds, grains and their s, legume, food and their raw Determination the level nitrogen and the level protein; Dumas NL/30 in accordance with ISO (Oleaginous seeds and feed) in accordance with ISO/TS (Grains and their s and legume) (remaining) analyse determination in accordance with ISO/TS Feed and their raw Determination the level raw fibre; gravimetric NL/32 in accordance with ISO Feed and their raw, grains and their s and oleaginous seeds Determination the fat content; direct extraction, gravimetric NL/33 equivalent to ISO 6492 (Feed and their raw ) equivalent to ISO (Grains and their s and feed) equivalent to ISO 659 (Oleaginous seeds) 25 Feed and their raw, grains and their s Determination the fat content; acidic hydrolysis; gravimetric NL/33 equivalent to ISO 6492 (Feed and their raw ) equivalent to ISO (Grains and their s and feed) 26 Feed and their raw, grains, legume and bys Determination the level ash; gravimetric NL/35 in accordance with ISO 5984 (Feed and ) in accordance with ISO 2171 (Grains and their s, legume and by- s) 27 Feed and their raw Determination the level insoluble ash in HCl(sand); gravimetric NL/36 in accordance with ISO Vegetable and Determination the content free fattyacid and acid value (FFA); titrimetric / potentiometric NL/38 in accordance with ISO 660 (titrimetric 9.1 potentiometric 9.2) Dutch Accreditation Council RvA Page 6 9
7 Annex to declaration accreditation (scope accreditation) Type activity 1 29 Vegetable and Determination water content; Karl Fischer method; titrimetric 30 Determination peroxide value; titrimetric NL/34d in accordance with ISO 8534 NL/40 in accordance with ISO Determination iodine value; titrimetric NL/41 in accordance with ISO Vegetable and animal oils and fats Determination moisture and volatile matter content; gravimetric NL/34e equivalent to ISO Animal oils, fats (as a basis for petroleum s) Determination nitrogen content; chemiluminescentie NL/31 in accordance with ASTM D4629 & ASTM D Vegetable oils, fats Determination nitrogen content; chemiluminescentie NL/31 Inorganic analyses 35 Vegetable and Determination the content fluoride; spectrophotometry NL/24 36 Food and feed and (solid matter) Determination the content As, Cd, Hg, Pb; microwave digestion and ICP-MS NL/26 (digestion) in accordance with EN in accordance with EN Determination the content Be, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Se, Sr, Mo, Ag, Sn, Sb, Ba, Tl; microwave digestion and ICP- MS NL/26 (digestion) in accordance with EN Dutch Accreditation Council RvA Page 7 9
8 Annex to declaration accreditation (scope accreditation) Type activity 1 38 Food and feed and (vegetable and ) Determination the content Na, Mg, Al, P, K, Ca, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Mo, Ag, Cd, Sn, Sb, Ba, Hg en Pb; ICP-MS NL/27 Microbiological analyses 39 Food and feed and Enumeration micro-organisms at 30ºC; PCA, Colony-count technique NL/M09 in accordance with ISO Enumeration coliforms at 37ºC; VRBL, Colony-count technique 41 Enumeration -glucuronidase-positive E.coli at 44ºC; TBX, Colony-count technique NL/M08 in accordance with ISO 4832 NL/M13 in accordance with ISO Food and feed and Enumeration Enterobacteriaceaeat 37ºC; VRBG, Colony-count technique NL/M14 in accordance with ISO Food and feed and with water activity < 0,95% Enumeration Moulds and Yeasts at 25ºC; DG-18, Colony-count technique NL/M12 in accordance with ISO Food and feed and Enumeration Bacillus cereus at 30 ºC; MYP, Colony-count technique NL/M11 in accordance with ISO Tap water Detection and enumeration Legionella; membrane filtration NL/M07 in accordance with NEN Food and feed and Detection Salmonella spp.; presence/absence reaction, immunologic quicktest NL/M01 equivalent to ISO 6579 (AFNOR BIO 12/16-09/05) 47 Detection Salmonella spp.; presence/absence reaction, real time PCR NL/D005 equivalent to ISO 6579 (MicroVal LR42) Dutch Accreditation Council RvA Page 8 9
9 Annex to declaration accreditation (scope accreditation) Type activity 1 48 Food and feed and Detection Salmonella spp.; presence/absence reaction, real time PCR NL/D Milk and milk powder, infant food, lecithin and lecithin containing s Detection Enterobacteriaceae spp; presence/absence reaction, real time PCR NL/D004 equivalent to ISO (MicroVal 2007LR ) 50 Milk powder, infant food, lecithin and lecithin containing s Detection Cronobacter spp (Enterobacter sakazakii); presence/absence reaction, real time PCR NL/D004 equivalent to ISO/TS (MicroVal 2007LR ) Molecularbiological analyses 51 Singular and pure raw /crops (such as soybean, Screening for Genetically Modified Organisms/crops (GMO): PCR 5 endonuclease instrument 52 maize, rice, sugar beet, flax seed, rape seed, potato s and cotton) Screening for Genetically Modified Organisms/crops (GMO): qpcr GMO-elements: 35S, NOS, PAT, FMV, NPTII, CTP2:CP4 EPSPS, BAR, CryIAb 53 Soybeans Quantitative analysis Roundup Ready Soy (GMO): qpcr 5 endonuclease instrument NL/D001 en NL/D002 NL/D001 en NL/D002 NL/D003 Flexible scope Organic analyses 2 54 Food and their raw Determination the content pesticides and additives; GC-MS/MS and LC-MS/MS NL/10a, NL/10b, NL/10c 55 Feed and their raw, vegetable and Determination the content pesticides and additives; GC-MS/MS and LC-MS/MS NL/10a, NL/10b, NL/10c 2 This flexible scope requires the laboratory to maintain a current list the methods applied under this flexible scope. Dutch Accreditation Council RvA Page 9 9
This annex is valid from: to Replaces annex dated: Location(s) where activities are performed under accreditation
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