Cereals and genetically modified crops

Transcription

1 New NIM-GBW10011 Wheat flour - Trace elements Ag ± % As ± mg/kg Ba ± 0.3 mg/kg Ca ± % Cd ± 4 μg/kg Ce ± mg/kg Cl ± % Cr ± mg/kg Cu ± 0.2 mg/kg Fe ± 3.1 mg/kg Gd μg/kg Indicative values for further elements K ± % La ± mg/kg Li ± mg/kg Mg ± % Mn ± 0.3 mg/kg Mo ± 0.05 mg/kg N ± 0.06 % Na ± 5 mg/kg Nd ± mg/kg Ni ± 0.02 mg/kg P ± % Cereals and genetically modified crops Wheat New ERM-BC382 NIST-1567a Wheat flour - Proximates and essential elements One unit contains about 37 g of wheat flour filled under argon in 100 ml amber glass s. Uncertainty Kjeldahl nitrogen g/100 g g/100 g Total fat g/100 g g/100 g Ash g/100 g g/100 g Starch g/100 g g/100 g K mg/g mg/g Mg mg/g mg/g Ca mg/g mg/g P mg/g mg/g Wheat flour - Trace elements Milled from a blend of Hard Red Spring and Hard Red Winter wheat. Al µg/g K % P % Ca % Mg % Rb µg/g Cd µg/g Mn µg/g S % Cu µg/g Mo µg/g Se µg/g Fe µg/g Na µg/g Zn µg/g Indicative values for As, Br, Cl, Co, Hg, I, Pb, Sn, U, V, W Pb ± mg/kg Pr ± 0.4 μg/kg Rb ± 0.2 mg/kg S ± % Se ± mg/kg Sm ± 0.28 μg/kg Sr ± 0.3 mg/kg V ± mg/kg Y ± mg/kg Zn ± 0.7 mg/kg 80 g 35 g BCR-121 BCR-563 BCR-396 BCR-471 New B-MYC0856 NIST-RM 8441a Wholewheat flour - Vitamins B1 (thiamin) mg/kg B6 (total pyridoxine) mg/kg Common wheat flour - Properties Folate (total) mg/kg Certified parameters determined according to ISO and ICC standard: Protein content, Ash content, Falling number/s, Zeleny sedimentation, Chopin Alveograph, Brabender Farinograph, Brabender Extensograph. Wheat flour - Deoxynivalenol (DON) blank Deoxynivalenol... <0.05 mg/kg Wheat - Ochratoxin A (blank) Ochratoxin A... < 0.6 µg/kg Wheat flour - Deoxynivalenol Deoxynivalenol ± 23 µg/kg Wheat - Hardness Set of 50 x 20 g Determination of hardness of bulk or single kernel wheat. Ten separate lots of wheat of varying hardness. Five samples of each wheat. 50 g 360 g 150 g 55 g 55 g set

2 Corn BCR-377 ERM-BC716 ERM-BC717 New B-MYC0851 New B-MYC0890 New B-MYC0891 New B-MYC0892 New B-MYC0893 New B-MYC0894 New B-MYC0895 IC-INCT-CF-3 Maize flour - Deoxynivalenol (blank) Deoxynivalenol... <0.05 mg/kg Maize - very low level ZON Compound Zearalenone...<5 μg/kg Maize - low level ZON Compound Uncertainty Zearalenone...83 μg/kg... 9 μg/kg Maize flour - Aflatoxins Aflatoxin B ± 0.37 μg/kg Aflatoxin B ± 0.07 µg/kg Aflatoxin G1... <LOD* Aflatoxin G2... <LOD* Aflatoxins total (sum of B1,B2,G1and G2) ± 0.26 µg/kg *The LOD for Aflatoxin G1 of the used method for in-house characterization of the material is 0.1 µg/kg, for Aflatoxin G2 the LOD is 0.1 µg/kg Maize flour check sample - Aflatoxins Indicative values Aflatoxin B ± 2.34 µg/kg Aflatoxin B ± 0.43 µg/kg Aflatoxin G1...<LOD* Aflatoxin G2...<LOD* Aflatoxins total (sum of B1,B2,G1 and G2) ± 2.63 µg/kg * The LOD for Aflatoxin G1 of the used method is 0.1 µg/kg, for Aflatoxin G2 the LOD is 0.1 µg/kg Maize flour check sample - Aflatoxins Indicative values Aflatoxin B ± 3.93 µg/kg Aflatoxin B ± 0.34 µg/kg Aflatoxin G1... <LOD* Aflatoxin G2... <LOD* Aflatoxins total (sum of B1,B2,G1 and G2) ± 4.05 µg/kg * The LOD for Aflatoxin G1 of the used method is 0.1 µg/kg, for Aflatoxin G2 the LOD is 0.1 µg/kg Maize flour check sample - Deoxynivalenol Indicative value Deoxynivalenol ± 290 µg/kg Maize flour check sample - Fumonisins Indicative values Fumonisin B ± 740 µg/kg Fumonisin B ± 340 µg/kg Maize flour check sample - Fumonisins Indicative values Fumonisin B ± 110 µg/kg Fumonisin B2... <80 µg/kg Maize flour check sample - Zearalenone Indicative value Zearalenone ± 64.8 µg/kg Corn flour - Trace elements Fumonisin B ± 210 µg/kg Fumonisin B3... <80 µg/kg B ± 0.33 mg/kg La ± 1.2 ng/kg P ± 97 mg/kg Br ± mg/kg Sc ± 0.32 ng/kg Rb ± mg/kg Cl ± 33 mg/kg Mg ± 37 mg/kg S ± 121 mg/kg Cu ± 0.13 mg/kg Mn ± 0.22 mg/kg Zn ± 0.76 mg/kg Fe ± 1.4 mg/kg Mo ± 12 ng/kg K ± 119 mg/kg Ni ± mg/kg Indicative values for Al, As, Ba, Ca, Hg, Na, Pb, Sb 150 g 60 g 60 g 55 g 100 g 100 g 100 g 100 g 100 g 100 g 50 g

3 New NIM-GBW10012 Maize flour - Trace elements 35 g Rye New ERM-BC381 IAEA-V-8 New B-MYC0852 Rice BCR-465 BCR-466 BCR-467 IRMM-804 Ag ± % As ± mg/kg B ± 0.11 mg/kg Ba ± 0.16 mg/kg Be ± 0.4 μg/kg Bi ± 0.9 μg/kg Br ± 0.09 mg/kg Ca ± % Cd ± 1.6 μg/kg Ce ± 0.02 mg/kg Cl ± % Cs ± mg/kg Cu ± 0.08 mg/kg Dy ± 0.8 μg/kg Er ± 0.4 μg/kg Indicative values for further elements Fe ± 1.5 mg/kg Gd ± 0.9 μg/kg Ho ± 0.15 μg/kg K ± % La ± mg/kg Li ± mg/kg Mg ± % Mn ± 0.08 mg/kg Mo ± mg/kg N ± 0.07 % Nd ± mg/kg Ni ± 0.014mg/kg P ± 0.003% Pb ± 0.02 mg/kg Pr... 7 ± 1 μg/kg Rye flour - Proximates and essential elements One unit contains about 37 g of rye flour filled under argon in a 100 ml amber glass. Uncertainty Kjeldahl nitrogen g/100 g g/100 g Total fat g/100 g g/100 g Ash g/100 g g/100 g Starch g/100 g g/100 g K mg/g mg/g Mg mg/g mg/g Ca mg/g mg/g P mg/g mg/g Rye flour - Trace elements Rb ± 0.2 mg/kg S ± % Sc ± 0.9 μg/kg Se ± mg/kg Si ± % Sm ± 0.5 μg/kg Sr ± 0.05 mg/kg Tb ± 0.24 μg/kg Th ± 1.5 μg/kg Ti ± 0.5 mg/kg V ± 0.11 mg/kg Y ± mg/kg Yb ± 0.2 μg/kg Zn ± 0.3 mg/kg Br mg/kg Fe mg/kg P mg/kg Ca mg/kg K mg/kg Rb mg/kg Cl mg/kg Mg mg/kg Zn mg/kg Cu mg/kg Mn mg/kg Indicative values for Cd, Co, Na Rye - Ergot alkaloids Ergometrine ± 745 µg/kg Ergometrinine ± 758 µg/kg Ergosine ± 260 µg/kg Ergosinine ± 476 µg/kg alpha-ergocryptine ± 52 µg/kg alpha-ergocryptinine ± 526 µg/kg Rice flour - Amylose (low level) Amylose g/100 g Rice flour - Amylose (medium level) Amylose g/100 g Rice flour - Amylose (high level) Amylose g/100 g Rice flour - Trace elements Compound Uncertainty (mg/kg) (mg/kg) As Cd Cu Mn Pb Zn Ergotamine ± 1156 µg/kg Ergotaminine ± 367 µg/kg Ergocornine ± 156 µg/kg Ergocorninine ± 391 µg/kg Ergocristine ± 195 µg/kg Ergocristinine ± 469 µg/kg 50 g 55 g 10 g 10 g 10 g 15 g

4 New NIM-GBW10010 Rice - Trace elements Ag ± % As ± mg/kg B ± 0.14 mg/kg Ba ± 0.09 mg/kg Be ± 0.4 μg/kg Br ± 0.13 mg/kg Ca ± % Cd ± 5 μg/kg Ce ± mg/kg Cl ± % Cs ± mg/kg Cu ± 0.3 mg/kg Indicative values for further elements Fe ± 1.9 mg/kg Hg ± 0.5 μg/kg K ± % La ± mg/kg Li ± mg/kg Mg ± % Mn...17 ± 1 mg/kg Mo ± 0.05 mg/kg N ± 0.04 % Na...25 ± 8 mg/kg Nb... mg/kg Ni ± 0.02 mg/kg Cereals and genetically modified crops NIST-1568a New NMIJ CRM 7503-A Rapeseed ERM-BC190 ERM-BC366 ERM-BC367 BCR-446 BCR-447 BCR-448 Rice Flour - Trace elements Produced from 100% long grain rice from Arkansas. Al µg/g Hg P % As µg/g K % Rb µg/g Ca % Mg % S % Cd µg/g Mn µg/g Se µg/g Cu µg/g Mo µg/g Zn µg/g Fe µg/g Na µg/g Indicative values for Br, Cl, Co, I, Pb, Sb, Sn, U, V, W White rice flour - Arsenic compounds and trace elements Arsenite (As(III)) ± mg/kg as As Arsenate (As(V)) ± mg/kg as As Dimethylarsinic acid (DMAA) ± mg/kg as As P ± 0.006P % Pb ± 0.03 mg/kg Pr ± 0.3 μg/kg Rb ± 0.3 mg/kg S ± % Se ± mg/kg Si ± % Sr ± 0.05 mg/kg Y ± mg/kg Zn ± 2 mg/kg Mn ± 0.4 mg/kg Cu ± 0.07 mg/kg As ± mg/kg Fe ± 0.21 mg/kg Zn ± 0.9 mg/kg Cd ± mg/kg Rapeseed (colza) - Total glucosinolate and sulfur Total glucosinolate...23 mmol/kg Rapeseed (colza) - Total glucosinolate and sulfur Total glucosinolate mmol/kg Rapeseed (colza) - Total glucosinolate and sulfur Total glucosinolate...99 mmol/kg Rapeseed - Oil content (low) As is oil g/100 g Moisture and volatiles g/100 g Dry basis oil g/100 g Rapeseed - Oil content (medium) As is oil g/100 g Moisture and volatiles g/100 g Dry basis oil g/100 g Rapeseed - Oil content (high) Genetically modified crops As is oil g/100 g Moisture and volatiles g/100 g Dry basis oil g/100 g ERM-BF410ak - ERM-BF410gk S g/kg S g/kg S g/kg Dried soya seed powders with different mass fractions of genetically modified ( Roundup ReadyTM ) soya beans, intended for the New ERM-BF410AK Soya seed powder GTS Soya (0%) 1 g GTS Soya... < 0.7 g/kg 80 g 35 g 20 g 20 g 20 g 20 g 153 g 153 g 153 g

5 New ERM-BF410BK Soya seed powder GTS Soya (0.1%) 1 g GTS Soya ERM-BF410C Genetically modified soya beans (0.5%) 1 g TM Roundup Ready Soya bean content New ERM-BF410DK Soya seed powder - GTS Soya (1%) GTS Soya New ERM-BF410GK Soya seed powder - GTS Soya (10 %) GTS Soya g 1 g ERM-BF425A - ERM-BF425D Dried soya seed powders with different mass fractions of genetically modified ( )soya beans, intended for the ERM-BF425A Genetically modified Soya seed (0%) Soya... < 0.5 ERM-BF425B Genetically modified Soya seed (0.1%) Soya ERM-BF425C Genetically modified Soya seed (1%) Soya ERM-BF425D Genetically modified Soya seed (10%) Soya ERM-BF426A - ERM-BF426D Dried soya seed powders with different mass fractions of genetically modified ( ) soya beans, intended for the ERM-BF426A Genetically modified Soya seed (0%) Soja... < 0.8 ERM-BF426B Genetically modified Soya seed (0.5%) Soya ERM-BF426C Genetically modified Soya seed (1%) Soya ERM-BF426D Genetically modified Soya seed (10%) Soya ERM-BF411A - ERM-BF411F Dried maize powders with different mass fractions of genetically modified ( Bt-176 ) maize, intended for the ERM-BF411A Genetically Modified Bt-176 Maize (0%) 1 g Bt-176 maize content...< 0.14 ERM-BF411B Genetically Modified Bt-176 Maize (0.1%) 1 g Bt-176 maize content ERM-BF411C Genetically Modified Bt-176 Maize (0.5%) 1 g Bt-176 maize content ERM-BF411D Genetically Modified Bt-176 Maize (1%) 1 g Bt-176 maize content

6 ERM-BF411E Genetically Modified Bt-176 Maize (2%) 1 g Bt-176 maize content ERM-BF411F Genetically Modified Bt-176 Maize (5%) 1 g Bt-176 maize content ERM-BF412A - ERM-BF412F Dried maize powders with different mass fractions of genetically modified ( Bt-11 ) maize, intended for the ERM-BF412A Genetically Modified Bt-11 Maize (0%) 1 g Bt-11 maize content... < 0.12 ERM-BF412B Genetically Modified Bt-11 Maize (0.1%) 1 g Bt-11 maize content ERM-BF412C Genetically Modified Bt-11 Maize (0.5%) 1 g Bt-11 maize content ERM-BF412D Genetically Modified Bt-11 Maize (1%) 1 g Bt-11 maize content ERM-BF412E Genetically Modified Bt-11 Maize (2%) 1 g Bt-11 maize content ERM-BF412F Genetically Modified Bt-11 Maize (5%) 1 g Bt-11 maize content ERM-BF413AK - ERM-BF413GK Dried maize powders with different mass fractions of genetically modified ( MON 810 ) maize, intended for the ERM-BF413AK Genetically modified MON-810 maize powder (blank) MON 810 maize mass fraction... < g ERM-BF413CK Genetically modified MON-810 maize powder (0.5%) MON 810 maize mass fraction ERM-BF413EK Genetically modified MON-810 maize powder (2%) Uncertainty MON 810 maize mass fraction g/kg g/kg MON 810 maize DNA copy number ratio % % ERM-BF413GK Genetically modified MON-810 maize powder (10%) MON 810 maize mass fraction g 1 g 1 g ERM-AD413 Plasmid DNA fragments of MON 810 maize Uncertainty Fragment of 5' plant-p35s junction DNA / plasmid negligible Fragment of hmg DNA / plasmid negligible ERM-BF414A - ERM-BF414F Dried maize powders with different mass fractions of genetically modified ( GA21 ) maize, intended for the ERM-BF414A Genetically modified GA21 Maize (0%) GA21 maize content... < 0.8 ERM-BF414B Genetically modified GA21 Maize (0.1%) GA21 maize content ERM-BF414C Genetically modified GA21 Maize (0.5%) GA21 maize content

7 ERM-BF414D Genetically modified GA21 Maize (1%) GA21 maize content ERM-BF414E Genetically modified GA21 Maize (2%) GA21 maize content ERM-BF414F Genetically modified GA21 Maize (5%) GA21 maize content ERM-BF415A - ERM-BF415F Dried maize powders with different mass fractions of genetically modified ( NK603 ) maize, intended for the ERM-BF415A Genetically modified NK603 Maize (0%) NK603 maize content...< 0.4 ERM-BF415B Genetically modified NK603 Maize (0.1%) NK603 maize content ERM-BF415C Genetically modified NK603 Maize (0.5%) NK603 maize content ERM-BF415D Genetically modified NK603 Maize (1%) NK603 maize content ERM-BF415E Genetically modified NK603 Maize (2%) NK603 maize content ERM-BF415F Genetically modified NK603 Maize (5%) NK603 maize content ERM-BF416A - ERM-BF416D Dried maize powders with different mass fractions of genetically modified ( MON 863 ) maize, intended for the ERM-BF416A Genetically modified MON 863 Maize (0%) 1 g/kg MON 863 Maize...< 1.0 1) No contamination was detected in the non-gm material when applying event-specific MON 863 realtime PCR with a detection limit of 0.8 g / kg. W ith a confidence level of 95 % the MON 863 mass fraction is below the certified value. ERM-BF416B Genetically modified MON 863 Maize (0.1%) MON 863 Maize ; +1.0 ERM-BF416C Genetically modified MON 863 Maize (1%) MON 863 Maize ; +1.2 ERM-BF416D Genetically modified MON 863 Maize (10%) MON 863 Maize ; +2.5 ERM-BF417a - ERM-BF417D Dried maize powders with different mass fractions of genetically modified ( MON 863 x MON 810 ) maize, intended for the ERM-BF417A Stacked Genetically modified MON 863 x MON 810 Maize 1 g/kg MON 863 x MON 810 Maize...< 1.0 1) No contamination was detected in the non-gm material when applying event-specific MON 863 realtime PCR with a detection limit of 0.8 g/kg and no contamination was detected when applying eventspecific MON 810 realtime PCR with a detection limit of 1.1g /kg. With a confidence level of 95 % the MON 863 x MON 810 maize mass fraction is below the certified value.

8 ERM-BF417B ERM-BF417C ERM-BF417D Stacked Genetically modified MON 863 x MON 810 Maize MON 863 x MON 810 Maize ; +1.0 Stacked Genetically modified MON 863 x MON 810 Maize MON 863 x MON 810 Maize ; +1.2 Stacked Genetically modified MON 863 x MON 810 Maize MON 863 x MON 810 Maize ; +2.4 ERM-BF418A - ERM-BF418D Dried maize powders with different mass fractions of genetically modified ( 1507 ) maize, intended for the ERM-BF418A Genetically modified 1507 Maize (0%) 1 g/kg 1507 maize... < 0.5 1) No contamination was detected in the non-gm material when applying event-specific 1507 real-time PCR with a detection limit of 0.4 g/kg. With a confidence level of 95 % the 1507 maize mass fraction is below the certified value. ERM-BF418B Genetically modified 1507 Maize (0.1%) 1507 maize ; +0.6 ERM-BF418C Genetically modified 1507 Maize (1%) 1507 maize ; +0.8 ERM-BF418D Genetically modified 1507 Maize (10%) 1507 maize ; +2.0 ERM-BF420A - ERM-BF420C Dried maize powders with different mass fractions of genetically modified Event 3272 maize intended for the ERM-BF420A Genetically modified 3272 Maize (0%) Event 3272 maize... < 1.3 ERM-BF420B Genetically modified 3272 Maize (1%) Event 3272 maize ERM-BF420C Genetically modified 3272 Maize (10%) Event 3272 maize ERM-BF423A- ERM-BF423D Dried maize powders with different mass fractions of genetically modified ( MIR604 ) maize, intended for the ERM-BF423A Genetically modified MIR604 Maize (0%) 1 g/kg MIR604 maize... < 0.9 1) No contamination was detected in the non-gm material when applying event-specific MIR604 real-time PCR with a detection limit of 0.8 g/kg. W ith a confidence level of 95 % the MIR604 maize mass fraction is below the certified value. ERM-BF423B Genetically modified MIR604 Maize (0.1%) MIR604 maize ; +1.0 ERM-BF423C Genetically modified MIR604 Maize (1%) MIR604 maize ; +1.3 ERM-BF423D Genetically modified MIR604 Maize (10%) MIR604 maize ; +2.9 ERM-BF424A - ERM-BF424D Dried maize powders with different mass fractions of genetically modified ( ) maize, intended for the

9 ERM-BF424A Genetically modified Maize (0%) 1 g/kg maize... < 1.2 1) No contamination was detected in the non-gm material when applying event-specific real-time PCR with a detection limit of 1.0 g/kg. With a confidence level of 95 % the maize mass fraction is below the certified limit value. The certified value is traceable to the SI. ERM-BF424B Genetically modified Maize (0.1%) maize ; +1.2 ERM-BF424C Genetically modified Maize (1%) maize ; +1.4 ERM-BF424D Genetically modified Maize (10%) maize ; +5.9 New ERM-BF427A ERM-BF427A - ERM-BF427D Dried maize seed powders with different mass fractions of genetically modified ( ) maize seed, intended for the Genetically modified Maize seed (blank) Maize... < 0.4 New ERM-BF427B Genetically modified Maize seed (0.5%) Maize New ERM-BF427C Genetically modified Maize seed (2%) Maize New ERM-BF427D Genetically modified Maize seed (10%) Maize ERM-BF421A- ERM-BF421B Dried potato powder powders with different mass fractions of genetically modified ( EH ) potato, intended for the ERM-BF421A Genetically modified EH Potato (0%) ERM-BF421a is a blank material composed of milled, dried powder from solely non-eh potatoes. The number fraction is calculated by counting the number of non-modified potatoes and the number of EH potatoes and expressing the result as a percentage. Each individual potato was tested for the presence of the genetic modification by staining a small potato piece for the presence of amylose (Lugol s test). The genetic identification is based on event-specific real-time PCR for EH Certified property Number fraction of EH potatoes / total number of potatoes [%] Genetic identity... Potato without the EH event 2 1 Based on the experimental confirmation of the absence of the EH genetic modification in every individual potato used for the processing through colorimetric analysis of the amylose content in a surface slice. The uncertainty is considered zero. 2 Based on event-specific real-time PCR analysis. No uncertainty is applicable. ERM-BF421B Genetically modified EH Potato (100%) ERM-BF421b is composed of milled, dried powder from purely EH potatoes and its genetic identity has been confirmed by nucleotide sequencing. The number fraction is calculated by counting the number of non-modified potatoes and the number of EH potatoes and expressing the result as a percentage. Each individual potato was tested for the presence of the genetic modification by staining a small potato piece for the presence of amylose (Lugol s test). The genetic identification is based on event-specific real-time PCR for EH and nucleotide sequencing around the junction region between the transgene insert and the potato genome. Certified property Number fraction of EH potatoes / total number of potatoes [%] Genetic identity... EH potato 2 1.Based on the experimental confirmation of the presence of the EH genetic modification in every individual potato used for the processing through colorimetric analysis of the amylose content in a cut tuber surface slice. The uncertainty is considered zero. 2 Based on event-specific real-time PCR analysis and nucleotide sequence analysis of the junction region between the transgene DNA insert and the potato genome in EH No uncertainty is applicable.

10 ERM-BF419A - ERM-BF419B Dried sugar beet powders with different mass fractions of genetically modified ( H7-1 ) sugar beet, intended for the ERM-BF419A Genetically modified H7-1 Sugar Beet (blank) ERM-BF419a is available in glass bottles containing approximately 1 g of sugar beet powder closed under argon atmosphere. This reference material has been produced from non-modified sugar beet roots delivered by KW S (Einbeck, DE). According to Commission Regulation (EC) No 65/2004 the event H7-1 sugar beet corresponds to the unique identifier KM-ØØØH71-4. The certified H7-1 mass fraction is based on the mass fraction of non-modified sugar beet roots used for the processing of the CRM. All sugar beet roots used in the processing were individually tested by ELISA and eventspecific rt-pcr. Certified property 1 H7-1 sugar beet...0 g/kg 1) No contamination was detected in the non-gm material when applying event-specific H7-1 real-time PCR and ELISA targeting the 5-enolpyruvylshikimate-3-phosphate synthase protein on each individual sugar beet root used for processing. The certified value is traceable to the SI. ERM-BF419B Genetically modified H7-1 Sugar Beet (level 1) ERM-BF419b is available in glass bottles containing approximately 1 g of sugar beet powder closed under argon atmosphere. This reference material has been produced from genetically modified sugar beet roots delivered by KW S (Einbeck, DE). According to Commission Regulation (EC) No 65/2004 the event H7-1 sugar beet corresponds to the unique identifier KM-ØØØH71-4. The certified H7-1 mass fraction is based on the mass fraction of modified sugar beet roots used for the processing of the CRM. All sugar beet roots used in the processing were individually tested by ELISA and eventspecific rt-pcr. Certified property 1 H7-1 sugar beet g/kg 1) No contamination was detected in the non-gm material when applying event-specific H7-1 real-time PCR and ELISA targeting the 5-enolpyruvylshikimate-3-phosphate synthase protein on each individual sugar beet root used for processing. The certified value is traceable to the SI. ERM-BF422A- ERM-BF422D Dried cotton seed powders with different mass fractions of genetically modified ( x ) cotton seed, intended for the ERM-BF422A Genetically modified x Cotton seed (0%) x Cotton seed...< g/kg 1) No contamination was detected in the non-gm material when applying real-time PCR methods targeting the sequence and the sequence. The certified value is traceable to the SI. ERM-BF422B Genetically modified x Cotton seed (100%) x Cotton seed...> 979 ERM-BF422C Genetically modified x Cotton seed (1%) x Cotton seed ERM-BF422D Genetically modified x Cotton seed (10%) x Cotton seed New ERM-BF428A ERM-BF428A - ERM-BF428C Dried cotton seed powders with different mass fractions of genetically modified ( GHB119 ) cotton seed, intended for the Genetically modified GHB119 Cotton seed (blank) GHB119 cotton... < 0.2 New ERM-BF428B Genetically modified GHB119 Cotton seed (1%) GHB119 cotton New ERM-BF428C Genetically modified GHB119 Cotton seed (10%) GHB119 cotton New ERM-BF429A ERM-BF429A - ERM-BF429C Dried cotton seed powders with different mass fractions of genetically modified ( T ) cotton seed, intended for the Genetically modified T Cotton seed (blank) T cotton... < 0.4

11 New ERM-BF429B Genetically modified T Cotton seed (1%) T cotton New ERM-BF429C Genetically modified T Cotton seed (10%) T cotton

12