47 th Tobacco Workers Conference. January 11-14, 2016 Sheraton Music City Hotel Nashville, Tennessee USA

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1 47 th Tobacco Workers Conference January 11-14, 2016 Sheraton Music City Hotel Nashville, Tennessee USA

2 GENERAL PROGRAM Monday, January 11, :30-5:30 pm Registration... Plantation Lobby 12:30-5:30 pm Speaker Ready Room... Belmont Room Committee Meetings 9:00-10:00 am Burley and Dark Tobacco Production Guide Working Group...Kingsley Room 10:00-12:00 pm Flue-Cured Tobacco Disease Resistance Evaluation Committee... Two Rivers Room 12:00-2:00 pm Flue-Cured Variety Evaluation and Minimum Standards Committees... Two Rivers Room 2:00-3:00 pm Burley Variety Evaluation Committee... Two Rivers Room 3:00-4:00 pm Flue-Cured & Burley Regional Growth Regulator Committee...Kingsley Room 4:00-5:00 pm Tobacco Pesticide Committee... Two Rivers Room 5:00-5:15 pm Nominating Committee... Edgewood Room 5:15-5:30 pm Resolutions Committee...Kingsley Room 5:30-6:00 pm Executive Committee... Edgewood Room Welcome Reception 6:00-7:30 pm Hosted by The University of Tennessee... Plantation Lobby Tuesday, January 12, :30-8:45 am Breakfast...McGavock s Ballroom 7:30-5:00 pm Registration... Plantation Lobby 7:30-5:00 pm Speaker Ready Room... Belmont Room 9:00-11:40 pm General Session... Hermitage Ballroom Salons B & C 10:25-10:55 am Break 11:40-1:00 pm Lunch on your own 1:00-3:00 pm Technical Sessions - Hermitage Ballroom - Session A: - Fertility... Salon B - Session B: Tobacco Disease Council - Black Shank... Salon C - Session C: Entomology - Future Directions for Tobacco IPM...Salon D 3:00-3:30 pm Break 3:30-5:30 pm Technical Sessions - Hermitage Ballroom - Session A: - Fertility, Transplant Production, and Physiology...Salon B - Session B: Tobacco Disease Council - Foliar Diseases, Nematodes, Bacterial Wilt... Salon C - Session C: Entomology - Insecticides...Salon D 2

3 Wednesday, January 13, :30-8:45 am Breakfast... McGavock s Ballroom 7:30-5:00 pm Registration... Plantation Lobby 7:30-5:00 pm Speaker Ready Room... Belmont Room 9:00-5:00 pm Poster Viewing...Salon E 9:00-10:40 am Technical Sessions - Hermitage Ballroom - Session A: - Weed Control, Market Preparation & Quality...Salon B - Session B: Agricultural Economics... Salon C - Session C: - Molecular Biology...Salon D 10:40-11:10 am Break & Poster Viewing (Salon E) 11:10-12:10 pm Technical Sessions - Hermitage Ballroom - Session A: Joint Session - & Agricultural Engineering...Salon B - Session B: Agricultural Economics... Salon C - Session C:... Salon D 12:10-1:30 pm Recognition Lunch...McGavock s Ballroom 1:30-2:00 pm Poster Viewing...Salon E 2:00-3:20 pm Technical Sessions. - Session A: Sucker Control...Salon B - Session B: Agricultural Economics... Salon C 3:20-3:40 pm Break & Poster Viewing...Salon E 3:40-4:40 pm Section Meetings - Agricultural Economics & Policy... Salon C - Agricultural Engineering...Salon D -...Salon B - Entomology... Edgewood Room - Tobacco Disease Council...Kingsley Room 4:40-5:40 pm General Business Meeting... Salon C Thursday, January, 14, :00-8:00 am Breakfast...McGavock s Ballroom 8:00-11:00 am Speaker Ready Room... Belmont Room 8:00-10:20 am Tobacco Breeding... Salon C 10:20-10:40 am Break 10:40-11:40 am Annual GAP Meeting (followed by private Board Meeting)... Salon C 3

4 47 th TOBACCO WORKERS CONFERENCE Tuesday, January 12, 2016 General Session - Hermitage Ballroom Presiding: Rob Ellis and Andy Bailey, University of Tennessee 9:00 Welcome Remarks: Rob Ellis, Co-Chair, 47th Tobacco Workers Conference 9:05 Melissa Bryant - Regional Director, Tennessee Farm Bureau 9:30 Tim Cross - Dean, University of Tennessee Extension 10:00 Julius Johnson - Commissioner of Agriculture, Tennessee Department of Agriculture 10:25 Break 10:55 The Evolving World of Tobacco: James Strickland, Altria Client Services 11:25 Tobacco Science Update: Bob Pearce, Editor 11:40 Adjourn Lunch on your own Session A - Salon B Fertility Presiding: Paul Denton GAP Connections 1:00 1. No-till and rotation improve soil aggregation and associated carbon and nitrogen stocks in burley tobacco production. B. Pearce; Zou, C.; Grove, J.; Coyne, M. University of Kentucky, Lexington KY USA 1:20 2. Residual nitrogen investigations for strip-tilled flue-cured tobacco (Nicotiana tabacum L.) production in Virginia. E. B. Brown, Reed, T. D.; Teutsch, C. D., Tracy, B. F., Virginia Tech, Blackstone VA USA Session B - Salon C Tobacco Disease Council Black Shank Presiding: Emily Pfeufer 32. Oxathiapiprolin, a new fungicide active ingredient (a.i.) for control of diseases caused by Oomycetes. V. Mascarenhas; Kuhn, P.; Mclean, H.; Tally, A.; Teeter, K.; Lowder, S., Syngenta Crop Protection, Greensboro NC USA 33. Black shank control in Virginia using Ridomil Gold and/or Presidio and/or Orondis in 2014 and C. S. Johnson, Virginia Tech, Blackstone VA USA Session C - Salon D Entomology Future Directions for Tobacco IPM Presiding: Hannah Burrack North Carolina State University 44. Future directions for integrated pest management in tobacco. H. J. Burrack, Toennisson, T. A., North Carolina State University, Raleigh 45. Overcoming barriers to integrated pest management adoption in flue-cured tobacco. J. D. Slone; Burrack, H. J., North 4

5 Tuesday, January 12, 2016 (continued) Session A - Salon B Session B - Salon C Session C - Salon D 1:40 3. Evaluation of NCDA boron sufficiency ranges for flue-cured tobacco. C. Jernigan; Hardy, D. H.; Hicks, K. A.; McGinnis, M. S., North Carolina Department of Agriculture and Consumer Services, Raleigh ; Fisher, L. R.; Vann, M. C., North 2:00 4. Dark fire-cured tobacco response to boron. J. C. Rodgers and W. A. Bailey, University of Kentucky, Princeton KY USA 2:20 5. Dark fire-cured tobacco response to potassium application rate and timing. R. A. Hill; Richmond, M. D.; Bailey, W. A.; Rodgers, J. C.,, Princeton KY USA 2:40 6. The effects of carbon nanosol on potassium adsorption characteristics and potassium uptake in the roots of tobacco. Liang, T. B.; LI, Y. L.; Yang J.; Zhang,Y.L.; and Yin, Q.S.; Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China 3:00 Break 34. Evaluation of Presidio and Ridomil Gold for black shank control in dark fire-cured tobacco. A. B. Keeney; Bailey, W. A.; Rodgers, J. C.; Hill, R. A.,, Princeton KY USA 35. Adaptation by Phytophthora nicotianae to partial resistance in tobacco. K. L. McCorkle; Lewis, R. S.; Shew, H. D., North Carolina State University, Raleigh 36. Nicotiana natural products inhibit black shank disease in tobacco. A. Mihaylova-Kroumova; Artiouchie, I.; Wagner, G. J.,, Lexington KY USA 37. Investigations into the use of stevia as a rotation crop in tobacco production systems. A. M. Koehler; Shew, H. D., North Carolina State University, Raleigh 46. Understanding interactions between systemic insecticides and generalist predators in tobacco. P. N. Nelson; Burrack, H. J.; Sorenson, C.E., North Carolina State University, Raleigh 47. Assessing the impact of flower strips on biological control of insects in organic tobacco. J. T. Klein; Burrack, H. J.; Toennisson, A., North Carolina State University, Raleigh 48. Insect management in tobacco grown for biofuel. T. A. Toennisson; Slone, J. D.; Burrack, H. J., North Carolina State University, Raleigh 5

6 Tuesday, January 12, 2016 (continued) Session A - Salon B Fertility, Transplant Production, and Physiology Presiding: Barry Sims University of Tennessee 3:30 7. Post-layby nitrogen application to fine textured soil of the North Carolina Piedmont. J. H. Mason; Vann, M. C.; Fisher, L. R., North 3:50 8. Evaluation of three transgenic varieties compared to three conventional varieties for low alkaloid production with three nitrogen variables. J. Cheek; Vann, M. C.; Fisher, L. R., North 4:10 9. Organic production of tobacco transplants: The nitrogen dilemma. B. Pearce,, Lexington KY USA 4: Evaluation of a hard plastic greenhouse float tray for tobacco transplant production. T. D. Reed, Virginia Tech, Blackstone VA USA 4: Multiplex use on tobacco to determine leaf major components, and maturation. E. Bargiacchi; Milli, G.; Romani, A.; Campo, M.; Miele, S., Italian Interuniversity Consortium for Science and Technology of Materials, Firenze Italy Session B - Salon C Tobacco Disease Council Foliar Diseases, Nematodes, Bacterial Wilt Presiding: Kenny Seebold Valent USA 38. Multimedia tools for understanding target spot disease dynamics. A. Mendoza-Moran; Shew, H.D, North Carolina State University, Raleigh 39. Simplifying and characterizing a blue mold resistance locus using next generation sequencing. K. Dluge; Dewey, R., North 40. Evaluation of fluensulfone for nematode management in flue-cured tobacco. A. S. Csinos; Hickey, H.; LaHue, S.; Hargett, U.; Gine, P. N., University of Georgia, Tifton GA USA 41. Effect of fatty alcohol concentration and formulation on mechanical transmission of bacterial wilt at harvest. P. D. Peterson; Fortnum, B.A., Clemson University, Florence SC USA 42. Novel compounds for the control of Ralstonia solanacearum. H. T. Tseng; Mila, A. L., North Carolina State University, Raleigh Session C - Salon D Entomology Insecticides Presiding: Hannah Burrack North Carolina State University 49. The use of imidacloprid affects two parasitoids of the tobacco budworm differently. C. E. Sorenson; Taylor, S. V.; Burrack, H. J.; Roe, M. R., North 50. DuPont Verimark AND Exirel insect control: novel insecticides for crop protection and yield optimization in tobacco. H. E. Portillo; Williams, R.W.; Smith, J.D.; Backscheider, K. A., DuPont Crop Protection, Newark DE USA 6

7 Tuesday, January 12, 2016 (continued) Session A - Salon B 5: Effects of light intensity on soluble sugars and organic acids in tobacco leaf (Nicotiana tabacum L.). B. Cai; Zhao, H.; Cai K.; Lei, B.; Guizhou Academy of Tobacco Science, Guizhou China 5:30 Adjourn Session B - Salon C 43. Sensitive detection of Ralstonia solanacearum in tobacco-planting soil with locked nucleic acid-based quantitative real-time PCR assay. Hu, L. W.; Xi, J. Q.; Liu, F.; Song, J. ZH., Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China; Feng, X. H.; Xiong, SH. B.; Wan, Y. F.; Zhang, R.; Zhang, H. G.; Zhou, G. W., Fuzhou Company of Jiangxi Tobacco Company, Fuzhou China 7

8 Wednesday, January 13, 2016 Poster Session Available for viewing in Salon E - 9:00 am - 5:00 pm 59. An evaluation of hybrid and pure line cultivars of flue-cured tobacco for cured leaf yield and quality. G. A. Amankwa; Mishra, S.; Shearer, A.; Van Hooren, D. L., Canadian Tobacco Research Foundation, Tillsonburg ON Canada 60. Management of blank shank on burley tobacco with oxathiapiprolin. C. Ammerman; Pearce, R.; Pfeufer, E.,, Lexington KY USA 61. Determining black shank resistance in tobacco varieties grown in Atkinson and Coffee counties in T. Barnes; von Waldner, M.; Edwards, P.; Bertrand, P.; Moore, J. M., University of Georgia, Pearson GA USA, Lewis, R., North 62. Preemergence Pythium damping-off of burley tobacco cultivar TN 90. L. A. Darnell; Johnson, C. S.; Reed, T. D., Virginia Tech, Blackstone VA USA 63. Management of tomato spotted wilt virus with imidacloprid in Lowndes County from J. Dawson; Kicklighter, J.; Price, J.; Moore, J. M.; and Bertrand, P., University of Georgia, Valdosta GA USA 64. Evaluation of fungicides for the control of blank shank and yield response on tobacco. B. A. Fortnum and P. D. Peterson, Clemson University, Pee Dee Research & Education Center, Florence SC USA 65. Greenhouse method to screen for resistance to Fusarium wilt in tobacco. N. Martinez and R. D. Miller,, Lexington KY USA 66. Tobacco-free campus initiative combined with carbon monoxide monitoring and counseling: quitting strategy for Kuwait College nursing student smokers. Omu, F. E.; Al-Kandari, I; Al-Marzouk, R; Paulraj, D; Rajagopal, M; John, P : College of Nursing, The Public Authority for Applied Education & Training, Safat Kuwait; Omu, A. E., Faculty of Medicine, Kuwait University, Kuwait 67. Update on the pasture herbicide stewardship program and efforts to reduce the occurrence of off-target damage to tobacco and other sensitive, high value crops. G. Neil Rhodes, Jr.; Israel, T. D.; McIntosh, D. W., University of Tennessee, Knoxville TN USA 68. Evaluation of MCW-2 (fluensulfone) as a nematicide in flue-cured tobacco production. C. Saude; Brammall, R.A.; Shearer, A.; and Van Hooren, D. L., Canadian Tobacco Research Foundation, Tillsonburg ON Canada 69. Chemical management of blank shank in Georgia. J. Shealey; von Waldner, M.; Barnes, T.; Bertrand, P.; Moore, J. M., University of Georgia, Statenville GA USA 70. Evaluating tobacco varieties for resistance to black shank. J. Taylor; Shealey, J.; Hollifield, S.; Shirley, B.; Bertrand, P.; and Moore, J. M., University of Georgia, Lakeland GA USA 8

9 Wednesday, January 13, 2016 (continued) 71. Management of spotted wilt in tobacco with imidacloprid in the northern portion of the Georgia flue-cured belt. C. Tyson; Earls, C., Griffin, C., Lanier, J., Spaid, D., Bertrand, P., Moore, J. M., University of Georgia, Reidsville GA USA 72. UGA Extension facilitated Good Agricultural Practices (GAP) tobacco farm safety and worker training. T. Varnedore and Moore, J.M., University of Georgia, Hazlehurst GA USA 73. Management of spotted wilt in tobacco with Actigard 50WG + imidacloprid. M. von Waldner; Barnes,T.; Beasley, E.; Curry, S.; Varnedore,T.; Bertrand, P. and Moore, J. M., University of Georgia, Douglas GA USA 74. Evaluation of tobacco varieties for resistance to black shank disease in Berrien County. E. Beasley; von Waldner, M.; Edwards, P.; Bertrand, P.; and Moore, J. M, University of Georgia, Nashville GA USA Session A - Salon B Weed Control, Market Preparation & Quality Presiding: Neil Rhodes University of Tennessee 9: Flue-cured tobacco layby herbicide evaluation. N. E. Harrell and B. M. Spivey, North Carolina Cooperative Extension Service, Wilson NC USA 9: Evaluation of non-tobacco labeled herbicides for late season application. W. T. Whaley; Fisher, L. R.; Vann, M.C., North Carolina State University, Raleigh 9: The influence of primary tillage and flue-cured tobacco management on Palmer amaranth populations in a three year crop rotation. M. C. Vann; Fisher, L. R., Jordan, D. L.; Stewart, A. M.; Wells, R., North Carolina State University, Raleigh Session B - Salon C Agricultural Economics Presiding: Will Snell Panel Discussion U.S. and Global Leaf and Tobacco Product Market Situation and Outlook Darryl Jayson, Tobacco Merchants Association Blake Brown, North Carolina State University Will Snell, Deqing Liang, China Tobacco (continued) Session C - Salon D Molecular Biology Presiding: Ling Yuan 51. High efficiency genome editing in Nicotiana tabacum using designer nucleases. R. Dewey; Wang, Y.; Smith, W.; Yuki Matsuba; North Carolina State University, Raleigh 52. Development of co-dominant markers for Nic1 and Nic2 in tobacco. Y. Shengming; Qin, Q.; Li, D.; Miller, R.; Jack, A.,, Lexington KY USA 53. Association mapping in a collection of tobacco reference cultivars. Step One: Variability of smoke constituents. E. Julio; SEITA; Verron, T.; Cahours, X.; Dorlhac de Borne, F.; Colard, S., Imperial Tobacco Group, Bergerac France 9

10 Wednesday, January 13, 2016 (continued) Session A - Salon B Session B - Salon C Session C - Salon D 10: Effect of artificial ordering on TSNAs in burley tobacco during short term bulking between takedown and stripping. C. R. Fisher; Jack, A.; Bush, L.; Pearce, R.,, Lexington KY USA 10: Lower leaf removal to eliminate lower stalk grades in flue-cured tobacco. L. R. Fisher; Vann, M. C.; Brown, A. B.; Inman, M. D., North Carolina State University, Raleigh 10:40 Break & Poster Viewing - Salon E & Agricultural Engineering Joint Session Presiding: David Reed Virginia Tech 11: Evaluation of a flue-cured tobacco bulk barn exhaust air heat recovery system. J. A. Macialek; Ellington, G. H., North 11: On-farm performance assessment of new curing barns. G. Ellington; Macialek, J. A., North Carolina State University, Raleigh 11: A proposed efficiency testing protocol for tobacco bulk curing barn heat exchangers. T. D. Reed, Virginia Tech, Blackstone VA USA 12:10 Recognition Luncheon 1:30 Poster Viewing - Salon E Panel Discussion Agricultural Economics (continued) Steve Pratt, Burley Tobacco Growers Association Daniel Green, Burley Stabilization Corporation Stuart Thompson, U. S. Tobacco Cooperative Agricultural Economics Presiding: Steve Isaacs Panel Discussion Farm Management/ Budgeting/GAP Issues Laura Powers, Gary Bullen, North Carolina State University Alan Galloway, University of Tennessee Jane Starnes, Paul Denton, GAP Connections Topping and suckercide treatments trigger global changes in gene expression in tobacco. S. Pattanaik; Singh, S. K.; Fisher, C.; Yuan, L., University of Kentucky, Lexington KY USA; Lawson, D., R. J. Reynolds, Inc., Winston-Salem 55. Biodesign of tobacco for high value products. J. S. Yuan; Texas A&M University/Synshark LLC, College Station TX USA Presiding: Ling Yuan 56. Genetic variability for upper stalk sucker growth in fluecured tobacco. J. L. Verrier and L Humeau, J., Bergerac Seed & Breeding, Bergerac, France 57. Field tests for tobacco gall nematodes resistance in France. J. L. Verrier and Mazeau, B., Bergerac Seed & Breeding, Bergerac, France 58. Effects of three primary tillage methods on flue-cured tobacco in North Carolina. M. D. Inman, Vann, M. C. and Fisher, L. R., North Carolina State University, Raleigh

11 Wednesday, January 13, 2016 (continued) Session A Salon B Session B Salon C Sucker Control Presiding: Eric Walker University of Tennessee 2: Using exogenous auxin application to control axillary shoot development in burley tobacco. W. J. Carmack; Miller, R. D.; Smalle, J. A.,, Lexington KY USA 2: Chemical topping of burley tobacco. M. D. Richmond; Bailey, W. A.; Pearce, R. C., University of Kentucky, Lexington KY USA 2: Comparison of sucker control methods for dark firecured tobacco. W. D. Pitt; Bailey, W. A.; Sims, B. D.; Walker, E. R., University of Tennessee, Springfield TN USA 3: Maleic hydrazide residues resulting from applications at three times of day and three rates of MH. J. M. Moore and S. LaHue, University of Georgia, Tifton GA USA 3:20 Poster Viewing - Salon E Agricultural Economics Presiding: Blake Brown North Carolina State University 3:40 Section Meetings - Agricultural Economics & Policy - Kingsley Room - Entomology - Edgewood Room - Agricultural Engineering - Salon D - Tobacco Disease Council - Salon C - - Salon B 4:40 General Business Meeting - Salon C Panel Discussion Policy Issues/ Regulatory Oversight Joe Cain, Kentucky Farm Bureau Jay Boyette, North Carolina Farm Bureau Graham Boyd, North Carolina Tobacco Growers Association Brandie Davis, Philip Morris International Mike Ligon, Universal Leaf Tobacco Company Darryl Jayson, Tobacco Merchants Association Orlando Chambers, Kentucky Tobacco Research & Development Center 11

12 Thursday, January 14, 2016 Session A - Salon C Tobacco Breeding Presiding: Bob Miller, University of Tennessee 8: Evaluation of androgenic and gynogenic doubled haploid lines for use as parental lines for hybrid burley tobacco varieties. R. D. Miller and E. DeOliveira,, Lexington KY USA 8: Optimal generation to develop tobacco doubled haploids when selecting for quantitatively inherited disease resistance. R. D. Miller and E. DeOliveira,, Lexington KY USA 8: Field evaluations of burley tobacco lines containing alleles minimizing nicotine to nornicotine conversion. C. G. Shelton and R. D. Miller,, Lexington KY USA 9: Breeding for increased nicotine content in tobacco. R. S. Lewis, North Carolina State University, Raleigh 9: Working towards an increased understanding of the genetics of soil-borne disease resistance in tobacco. K. Drake; and Lewis, R. S., North ; Bakaher, N.; Goepfert, S.; Philippon, B.; and Mark, R.; Philip Morris International, Neuchatel, Switzerland; Peterson, P. D; Clemson University, Florence SC USA 9: Generation, evaluation, and use of tobacco lines nearly isogenic for QTLS affecting black shank resistance. J. Ma, Lewis, R. S., North ; Edwards, K.; Humphry, M.; British American Tobacco, United Kingdom 10: The demethylase mutants - panacea or new problems? A. M. Jack; Ji, H.; Fannin, N.; Fisher, C.; Schoergendorfer, A.,, Lexington KY USA 10:20 Break 10:40 Annual GAP Meeting, moderated by Jane Starnes, GAP Connections 11:40 Adjourn 12

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