NNN Levels in Stable Reduced Converter (SRC) and LC lines cured under conditions that favor NNN formation.

Transcription

1 NNN Levels in Stable Reduced Converter (SRC) and LC lines cured under conditions that favor NNN formation. M. Lusso; K. Lion; A. Adams; W. Morris; U. Warek and J. Strickland Altria Client Services (ALCS) authors should use Altria Client Services LLC., Research, Development & Reg Affairs, 601 East Jackson Street, Richmond VA CORESTA

2 Introduction - TSNAs NNK NNN NAT NAB NNN and NNK have been identified by FDA as harmful or potentially harmful constituents in tobacco products 2

3 NNN Formation and ZYVERT TM Technology Nicotine Nicotine Demethylase Nicotine Conversion Nitrosation Nornicotine Nitrite NOx N -Nitrosonornicotine (NNN) Nitrate Fertilizer 3

4 NNN Formation and ZYVERT TM Technology Nicotine Nicotine Demethylase Nicotine Conversion Nitrosation Nornicotine Nitrite NOx N -Nitrosonornicotine (NNN) Nitrate Fertilizer 4

5 NNN Formation and ZYVERT TM Technology Nicotine Nicotine Demethylase Nicotine Conversion SRC Varieties ZYVERT TM Technology Nitrosation Nornicotine Nitrite NOx N -Nitrosonornicotine (NNN) Nitrate Fertilizer 5

6 Nornicotine Levels in SRC and LC Plant Populations SRC SRC Average = % LC LC Average = % Plant Number Plant Number 6

7 NNN Levels in SRC and LC Plant Populations SRC SRC Average = ppm Plant Number LC LC Average = 0.60 ppm Plant Number 7

8 Objective Evaluate the impact of ZYVERT TM technology in SRC Burley air-cured and SRC Dark fire-cured lines cured under conditions conducive to NNN formation Parameters: Barn Curing Conditions (RH or Temp) Nornicotine levels NNN levels 8

9 Experiment Design Burley Air-Cured Tobacco Varieties TN90 LC TN90 SRC with ZYVERT TM Technology 2 Curing Barns Control Barn Managed according to recommended curing practices 72 sticks (36 LC and 36 SRC) 6 replications Experimental Barn (Stressed) Managed to maintain high relative humidity during curing 96 sticks (48 LC and 48 SRC) 6 replications 9

10 Burley Air-Curing Barn Conditions - RH September October 10

11 Nornicotine (%) Nornicotine Levels Burley Air-Cured LC SRC Normal Curing -64% -58% Stressed Curing 11

12 NNN (ppm) NNN Levels Burley Air-Cured LC SRC Normal Curing -63% -76% Stressed Curing 12

13 Experiment Design Dark Fire-Cured Tobacco Varieties grown under standard dark production practices KY171 LC KY171 SRC with ZYVERT TM Technology 2 Curing Barns Control Barn Managed according to recommended curing practices Experimental Barn (Stressed) Managed to reach higher temperatures during fires 13

14 Maximum Temp (Degrees Fahrenheit) Fire-Curing Barn Conditions High Temperature Control Barn Stressed Barn st Fire 2nd Fire 3rd Fire 4th Fire 14

15 Fire-Curing Barn Conditions - Temperature Curing Conditions - Temperature 500 data point rolling average Control Stressed Max Temperature Reached - Control 137 F - Stressed 174 F /1 9/3 9/4 9/5 9/7 9/8 9/10 9/11 9/13 9/14 9/16 9/17 9/18 9/20 9/21 9/23 9/24 9/26 9/27 9/28 9/30 10/1 10/3 10/4 10/6 10/7 10/9 10/10 10/11 10/13 10/14 10/16 10/17 10/19 10/20 10/21 10/23 10/24 10/26 10/27 10/29 10/30 Temp (deg F) 100 September October

16 Nornicotine (%) Nornicotine Levels Dark Fire-Cured LC SRC % -70% Normal Curing Stressed Curing 16

17 NNN (ppm) NNN Levels Dark Fire-Cured LC SRC % Normal Curing -61% Stressed Curing 17

18 Conclusions Burley and dark SRC tobaccos with ZYVERT TM technology exhibit significant reductions in nornicotine and NNN levels compared to LC variety when cured under recommended conditions When cured under conditions conducive to NNN formation, burley and dark SRC tobaccos with ZYVERT TM technology continue to show significant reductions compared to controls Curing conditions have a significant impact on NNN formation Regardless of curing conditions, SRC varieties with ZYVERT TM Technology reduce NNN levels relative to controls 18

19 Questions 19