Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK ISO/IEC 17025: 2005 Essentra Scientific Services Contact: Mr A Stutz Shaftesbury Avenue Tel: +44 (0) Jarrow Fax: +44 (0) Tyne and Wear alanstutz@essentra.com NE32 3UP Website: Testing performed at the above address only DETAIL OF ACCREDITATION CIGARETTES Chemical Tests Documented In-House Methods Mainstream Cigarette Smoke (ISO smoking regimes) Intense smoking regime (Canadian) for collection of smoke for further testing ISO smoking regime for collection of smoke for further testing ISO smoking regime for collection of smoke for further testing Total Particulate Matter and puff number Carbon Monoxide Nicotine Water Calculated value: Nicotine free dry particulate matter (NFDPM) ph SM/032 using Linear Smoking Machine SM/030 using Linear Smoking Machine SM/034 using Rotary Smoking Machine SM/010, SM/020, and SM/060 based on ISO 4387:2000 SM/050 based on ISO 8454:2007 SM/085 based on ISO 10315:2000 SM/080 based on ISO :1999 Determined from Total Particulate Matter, Nicotine and Water values SM/150 (in-house method) using smoke collected on Linear or Rotary Menthol SM/130 based on SO 13110:2012 using smoke collected on Linear Assessment Manager: JB1 Page 1 of 10
2 Benzo[a]pyrene SM/140 based on ISO 22634:2008 by GC-MS or GC-MS/MS using smoke collected on Linear or Rotary (ISO smoking regimes) Tobacco specific nitrosamines (TSNA s) Total oxides of nitrogen as nitric oxide Carbonyls: Acetone Acetaldehyde Acrolein n-butyraldehyde Methyl ethyl ketone Crotonaldehyde Formaldehyde Propionaldehyde Acetamide and acrylamide Ammonia Aromatic amines: 1-aminonaphthalene 2-aminonaphthalene 3-aminobiphenyl 4-aminobiphenyl SM/160 based on Coresta Reference Method No. 75 by LC-MS/MS using smoke collected on Linear Smoking Machines SM/190 using NOx analyser using smoke collected on Rotary Smoking Machines SM/170 based on Coresta Reference Method No. 74 by HPLC with UV detection using smoke collected on Rotary SM/250 (in-house method) using Linear SM/220 based on Health Canada Method T-101 using ion chromatography using smoke collected on Rotary Smoking Machines SM/260 (in-house method) using Linear Assessment Manager: JB1 Page 2 of 10
3 Aromatic amines: o-toluidine o-anisidine 2,6-dimethylaniline Selected aza-arenes: 2-amino-3-methyl-3H-imidazo-[4,5- f]quinoline 2-aminop-3-methyl-9H-pyrido[2,3- b]indole 3-amino-1-methyl-5H-pyrido[4,3- b]indole 3-amino-1,4-dimethyl-5Hpyrido[4,3-b]indole 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine 2-amino-6-methyldipyrido[1,2- a:3,3 -d]imidazole 2-amino-9H-pyrido[2,3-b]indole Caffeic acid Ethyl carbamate Hydrazine SM/280 in-house method using Linear SM/320 in-house method using Linear SM/300 in-house method using Rotary SM/310 in-house method using Linear SM/290 in-house method using GC/MS using smoke collected on Rotary Hydrogen cyanide SM/210 based on HCTM No. T-107 using continuous flow analyser using smoke collected on Linear or Rotary Assessment Manager: JB1 Page 3 of 10
4 Selected chlorinated dioxins and furans: 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) 1,2,3,7,8-pentachlorodibenzo-pdioxin (1,2,3,7,8-PCDD) 1,2,3,4,7,8-hexachlorodibenzo-pdioxin (1,2,3,4,7,8-HxCDD) 1,2,3,4,6,7,8-heptachlorodibenzop-dioxin (1,2,3,4,6,7,8-HpCDD) octachlorodibenzo-p-dioxin (OCDD) 1,2,3,6,7,8-hexachlorodibenzo-pdioxin (1,2,3,6,7,8-HxCDD) 1,2,3,7,8,9-hexachlorodibenzo-pdioxin (1,2,3,7,8,9-HxCDD) 2,3,7,8-tetrachlorodibenzofuran (2,3,7,8-TCDF) 1,2,3,7,8-pentachlorodibenzofuran (1,2,3,7,8-PCDF ) 1,2,3,4,7,8-hexachlorodibenzofuran (1,2,3,4,7,8-HxCDF) 1,2,3,4,6,7,8- heptachlorodibenzofuran (1,2,3,4,6,7,8-HpCDF) octachlorodibenzofuran (OCDF) 2,3,4,7,8-pentachlorodibenzofuran (2,3,4,7,8-PCDF) 1,2,3,6,7,8-hexachlorodibenzofuran (1,2,3,6,7,8-HxCDF) 1,2,3,7,8,9- hexachlorodibenzofuran (1,2,3,7,8,9-HxCDF) 2,3,4,6,7,8-hexachlorodibenzofuran (2,3,4,6,7,8-HxCDF) 1,2,3,4,7,8,9- heptachlorodibenzofuran (1,2,3,4,7,8,9-HpCDF) SM/270 in-house method using GC/MS using smoke collected on Rotary Assessment Manager: JB1 Page 4 of 10
5 Polycyclic aromatic hydrocarbons: Naphathalene Benzo[c]phenanthrene Benz[a]anthracene Benz[j]aceanthrylene Cyclopenta[cd]pyrene Chrysene 3-Methylcholanthrene 5-Methylchrysene Benzo[b]fluoranthrene Benzo[k]fluoranthrene Benzo[a]pyrene Indeno[1,2,3-cd]pyrene Dibenzo[a,l]pyrene Dibenzo[a,e]pyrene Dibenzo[a,i]pyrene Dibenzo[a,h]pyrene Dibenz[a,h]anthracene Semi-volatile organic compounds: Pyridine Styrene 2,3-Benzofuran Nitrobenzene Quinoline SM/200 based on ISO 22634:2008 (benzo[a]pyrene in smoke) by GC/MS-MS using smoke collected on Rotary SM/350 by GC-MS using smoke collected on Linear Smoking Machines Selected volatile nitrosamines: N-nitrosodimethylamine N-nitrosodiethylmethylamine N-nitrosodiethylamine N-nitrosopiperidine Selected volatile nitrosamines: N-nitrososarcosine N-nitrosodiethanolamine SM/330 in-house method by GC-MS using smoke collected on Rotary SM/340 in-house method using Linear Assessment Manager: JB1 Page 5 of 10
6 (ISO smoking regimes) Sidestream Cigarette Smoke (collected by ISO smoking regime) Volatile organic compounds: Vinyl chloride 1,3-Butadiene Ethylene oxide Propylene oxide Isoprene Furan Vinyl acetate Acrylonitrile Benzene Nitromethane 2-Nitropropane Toluene Ethyl benzene Phenols: Catechol m & p-cresol o-cresol Hydroquinone Phenol Resorcinol Smoking for collection of sidestream smoke for: SM/240 based on Coresta Reference Method No. 70 method by GC-MS using smoke collected on Rotary SM/087 (in-house method) using HPLC with fluorescence detection using smoke collected on Linear SS/005 using Linear Smoking Machine Carbon Monoxide SS/020 based on ISO 20774:2007 Tobacco specific nitrosamines (TSNA s) Carbonyls: Acetone Acetaldehyde Acrolein n-butyraldehyde Methyl ethyl ketone Crotonaldehyde Formaldehyde Propionaldehyde SS/040 based on Coresta Reference Method No. 75 by LC-MS/MS SS/050 based on Coresta Reference Method No. 74 by HPLC with UV detection Benzo[a]pyrene SS/060 based on ISO 22634:2008 by GC/MS-MS Assessment Manager: JB1 Page 6 of 10
7 Sidestream Cigarette Smoke Cigarette Filters Phenols: Catechol m & p-cresol o-cresol Hydroquinone Phenol Resorcinol Retention of Particulate Matter and Nicotine SS/030 (in-house method) using HPLC with fluorescence detection SM/010 SM/020 SM/040 SM/070 SM/080 SM/100 CIGARETTES Physical Tests Documented In-House Methods based on National and International Standards Cigarettes and Cigarette Filters Weight Pressure drop Paper Porosity Length Pressure drop/draw resistance Percentage ventilation Circumference/Diameter PM/040 based on ISO 4387:2000 PM/030 based on ISO 6565:2015 PM/050 based on ISO 2965:2009 PM/070 based on ISO 4387:2000 PM/015 based on ISO 6565:2015 ISO 9512:2002 and ISO 2971:1998 Cigarettes Ignition propensity IP/010 based on ISO 12863:2010 and ASTM E Assessment Manager: JB1 Page 7 of 10
8 SMOKELESS NICOTINE DELIVERY PRODUCTS (ecigarettes, electronic cigarettes, vaping devices) Smokeless nicotine delivery vapour / aerosol Chemical Tests Carbonyls: Acetone Acetaldehyde Acrolein n-butyraldehyde Methyl ethyl ketone Crotonaldehyde Formaldehyde Propionaldehyde Diacetyl Acetyl propionyl Water Nicotine Menthol Propylene Glycol Glycerol Anabasine Ethylene Glycol Diethylene Glycol Documented In-House Methods based on industry standard methods EM/0/20 vapour generation and collection followed by EM/040 based on Coresta Reference Methods No. 81 and 74 by UPLC with UV detection using smoke collected on Linear Smoking Machine EM 060 in house method using linear smoking machine and GC-MS, GC- TCD and GC-FID N-Nitrosonornicotine (NNN) N-Nitrosoanatabine (NAT) N-Nitrosoanabasine (NAB) 4-(N-nitrosomethylamino)-1-(3- pyridyl)-1-butanone (NNK) EM 080 in house method using linear smoking machine and LC-MS/MS Electronic cigarette liquid Chemical Tests Nicotine Water Menthol Glycerol Ethylene glycol Diethylene glycol Propylene glycol N-Nitrosonornicotine (NNN) N-Nitrosoanatabine (NAT) N-Nitrosoanabasine (NAB) 4-(N-nitrosomethylamino)-1-(3- pyridyl)-1-butanone (NNK EM/050 in-house method by GC with FID, TCD and MS detection EM 070 using LC-MS/MS Assessment Manager: JB1 Page 8 of 10
9 TOBACCO Chemical Tests Documented In-House Methods Moisture Reducing substances Polycyclic aromatic hydrocarbons: Benz[a]anthracene Chrysene Benzo[b]fluoranthrene Benzo[k]fluoranthrene Benzo[a]pyrene Indeno[1,2,3-cd]pyrene Dibenzo[a,h]pyrene Benzo[a]pyrene HT/05 by oven drying (in-house method) HT/130 by continuous flow analysis based on ISO 15153:2003 HT/140 based on Labstat International ULC Method SSPT30 by GC/MS-MS HT/150 based on Labstat International ULC Method SSPT30 by GC/MS-MS Total alkaloids Aflatoxin B1 Ammonia Carbonyls: Acetaldehyde Crotonaldehyde Formaldehyde Caffeic acid Coumarin Ethyl carbamate HT/160 by continuous flow analysis based on ISO 15152:2003 HT/180 (in-house method) by HPLC with pre-column derivatisation HT/20 by ion chromatography based on CORESTA method No.73 HT/60 in-house method by HPLC with UV detection HT/200 in-house method by HPLC HT/90 in-house method by LC/MS- MS HT/100 in-house method by LC/MS- MS Assessment Manager: JB1 Page 9 of 10
10 TOBACCO Chemical Tests Documented In-House Methods Tobacco and Tobacco Products Nicotine and alkaloids: Anatabine Anabasine Myosmine Nicotine Nornicotine ph Selected volatile nitrosamines: N-nitrosodimethylamine N-nitrosomorpholine N-nitrosopiperidine N-nitrosopyrrolidine Selected volatile nitrosamines: N-nitrososarcosine N-nitrosodiethanolamine Tobacco specific nitrosamines (TSNA s) Mercury Flavour, Humectant and Plasticiser compounds: Propylene Glycol Menthol n-decanol Triacetin Eugenol Glycerol Triethylene Glycol Nitrate Nitrite Sorbic Acid Propionic Acid HT/190 (in-house method) by GC/MS HT/10 following Aqueous extraction based on CORESTA method No.69 HT/70 in-house method by GC-MS HT/80 in-house method using LC/MS-MS HT/40 based on Coresta Reference Method No. 72 by LC-MS/MS HT/50 (in-house method) using DMA mercury analyser HT/210 based on Coresta Reference Method No. 60 method using GC/MS HT/220 in-house method using ion chromatography with conductivity and UV detection. END Assessment Manager: JB1 Page 10 of 10
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