Tracking X-ray microscopy for alveolar dynamics in live intact mice
|
|
- Anthony Bates
- 5 years ago
- Views:
Transcription
1 SUPPLEMENTARY INFORMATION Tracking X-ray microscopy for alveolar dynamics in live intact mice Soeun Chang, Namseop Kwon, Byung Mook Weon, Jinkyung Kim, Chin Kook Rhee, Han Sung Choi, Yoshiki Kohmura, Masaki Yamamoto, Tetsuya Ishikawa, and Jung Ho Je Supplementary Figure 1 Supplementary Figure 2 Supplementary Figure 3 Supplementary Figure 4 Supplementary Figure 5 Supplementary Figure 6 Supplementary Figure 7 Supplementary Figure 8 Supplementary Table 1 Supplementary Table 2 Supplementary Discussion 1 Supplementary Discussion 2 Overall lung movements Movement trajectories of individual alveoli during respiration Alveolar size (diameter) change correlated with the ventilator pressure Alveolar pressure-volume curves in mice The pressure size curves of two additional alveoli in two different mice Morphologies of duct and sac alveoli Morphological changes of individual alveoli in 3-D geometry during respiration Alveolar morphology Diameters of alveoli Morphologies of individual duct alveoli (DA) and sac alveoli (SA) Ratio of alveolar depth (D) to mouth diameter (MD) Radiation dose Note: Supplementary Videos 1-8 are available on the Scientific Reports website
2 Video Legends Supplementary Video 1. Real-time movie of the upper right lung lobe in a mouse during respiration with a normal tidal volume (160µl) (Supplementary Fig. 1). Here the overall lung movement during respiration is significant as more or less 300μm even for the upper right lobe with a minimum lung movement. Supplementary Video 2. Microtomographic movie of the upper right lung lobe of a mouse, taken at every end point of inspiration during 180º rotation of the sample stage (Supplementary Fig. 6a). Supplementary Video 3. Microtomographic movie of the upper right lung lobe of a mouse, taken at every end point of expiration during 180º rotation of the sample stage (Supplementary Fig. 6b). Supplementary Video 4. Real-time movie of individual alveoli from the end point of expiration to the end point of inspiration in the upper right lung lobe of a mouse (Supplementary Fig. 1a,b,c). Different movement trajectories of individual alveoli enabled us to recognize the coherent motion of optical features of each alveolus during respiration and as a result to identify and track individual alveoli from the overlapped images of numerous alveoli
3 Supplementary Video 5. Segmented microtomographic movie of one unit of branched ducts and sacs with alveoli in inspiration (Fig. 3a). Supplementary Video 6. Segmented microtomographic movie of one unit of branched ducts and sacs with alveoli in expiration (Fig. 3a). Supplementary Video 7. Segmented microtomographic movie of the upper right lung lobe of a mouse in inspiration (Supplementary Fig. 6c). Each color indicates each unit of branched ducts and sacs with alveoli. Supplementary Video 8. Segmented microtomographic movie of the upper right lung lobe of a mouse in expiration (Supplementary Fig. 6d). Each color indicates each unit of branched ducts and sacs with alveoli
4 Supplementary Figure 1 Representative real-time microradiographs of the upper right lung lobe of a mouse during a normal inspiration (0~200ms)/expiration (200~600ms) cycle with a normal tidal volume (160µl). The arrow indicates the alveolus at the apex top of the upper right lobe. Here the overall lung movement during respiration is significant as ~ 300 m even for the upper right lobe with a minimum lung movement. Scale bar, 100µm
5 Supplementary Figure 2 (a-c) Representative real-time microradiographs of the upper right lung lobe of a mouse from the end point of expiration (t 1 ) to the end point of inspiration (t 3 ). Different movement trajectories of individual alveoli enabled us to recognize the coherent motion of optical features of each alveolus during respiration and as a result to identify and track individual alveoli from the overlapped images of numerous alveoli. Each color (blue, green, red, and yellow) of the dashed circles indicates the same alveolus. (d) Trajectories (dashed arrows) of individual alveoli movements during respiration. Scale bar, 50µm. (e) The trajectories of 60 alveoli in 6 different mice during inspiration and expiration. The trajectories, defined here as the distance of individual alveolar positions in end of expiration and end of inspiration
6 Supplementary Figure 3 Alveolar size (diameter) change correlated with the ventilator pressure. (a) The ventilator pressure with time. (b) The size change of an alveolus with time (8ms interval) by real-time microradiography. Blinded analyses of the same alveolar images were done by five independent investigators, showing an inter-observer coefficient of variation of alveolar size measurements of <3%
7 Supplementary Figure 4 Alveolar pressure-volume curves in mice. Our data (red circles) clearly show a closed-loop hysteresis, similar to the whole-lung curve (inset) 1. We note that alveoli in live intact mice show lower compliances than reported for alveoli of thorax-incised mice (grey circles) 2. Here normalized volumes were estimated based on using alveolar size (our data of Fig. 2c) and alveolar area (IVM)
8 Supplementary Figure 5 The pressure size curves of four additional alveoli in four different mice during inspiration and expiration in the tidal volume ventilation. The alveolar sizes were normalized to the sizes measured at the end of expiration. The four alveoli show similar hysteresis behaviors. The size measurements were performed over 2 respiration cycles in each case
9 Supplementary Figure 6 Morphologies of duct and sac alveoli. (a-b) The microradiographs of the upper right lung lobe of a mouse in inspiration (a) and expiration (b). (c-d) The segmented 3D images corresponding to the images in (a) and (b), respectively. Each color indicates each unit of branched ducts with duct and sac alveoli. The diameter increments of individual alveolar ducts in respiration, estimated from the 3-D images in (c) and (d), are small as 4.3±1.3 % (mean ± s.e.m.). Scale bars, 100µm
10 Supplementary Figure 7 Morphological changes of individual alveoli in 3-D geometry during respiration. The ratio of the alveolar depth (D) and the mouth diameter (MD), D/MD; D/MD in sac alveoli (SA): 0.87±0.03 (mean±s.e.m.) in expiration (light gray) and 0.86±0.03(mean±s.e.m.) in inspiration (dark gray; P=0.8), D/MD in duct alveoli (DA): 0.59±0.03 (mean±s.e.m.) in expiration (light gray) and 0.59±0.03 (mean±s.e.m.) in inspiration (dark gray; P=1.0); *P=1.1x10-7 (**P=1.3x10-7 ) for D/MD between SA and DA in expiration (inspiration). n = ~80 alveoli over 18 ducts with sacs in a live intact mouse
11 Supplementary Figure 8 Alveolar morphology. (a-b) Alveolar schematics of hyper-hemisphere and hypo-hemisphere. (c-d) Representative 3D segmented images of alveolar epithelial surfaces for a sac alveolus (c) and a duct alveolus (d). The local distortion or flats on the surface in the 3-D segmented images are presumably due to adjacent alveoli and diversity in duct branch directions
12 Supplementary Table 1 Raw data of Fig. 2b. d 1 (d 2 ): diameter of individual alveoli in expiration (inspiration), I: diameter increment, and M: mouse number
13 Supplementary Table 2 Morphologies of individual duct alveoli (DA) and sac alveoli (SA) in 3-D geometry during respiration, measured for ~ 80 alveoli over 18 ducts with sacs in a live intact mouse (the errors correspond to the s.e.m.)
14 Supplementary Discussion 1. Ratio of Alveolar Depth (D) to Mouth Diameter (MD). The ratio D/MD is critically associated with the flow patterns within alveoli. One aspect of this factor can be thus exemplified by the study on flow trajectories of particles to predict deposition sites of inhaled pharmaceutical aerosols or pollutants in alveoli 3,4. Despite its importance, the reported D/MDs show a large variation from 0.4 to 2.4 3,4, mostly by the absence of real-time study for 3D alveoli imaging in live intact mice. The D/MD values (0.59±0.02 in ducts and 0.87±0.02 in sacs), obtained for live intact mice in this study, may greatly contribute to the understanding of flow patterns in alveoli 3,4. Supplementary Discussion 2. Radiation Dose. The high coherence of the monochromatic X-rays permitted to observe alveolar boundaries of live mice with high visibility by the edge-refraction enhancement 5 8. Actually the edge enhancement is very useful to reduce the radiation dose. In this study, the total radiation dose for one set (inspiratory triggering + expiratory triggering) of microtomography was reasonably small as ~ 2.5Gy 7, in spite of very high resolution 3-D imaging
15 References 1. Huang, K. et al. Variation in senescent-dependent lung changes in inbred mouse strains. J. Appl. Physiol. 102, (2007). 2. Mertens, M. et al. Alveolar dynamics in acute lung injury: Heterogeneous distension rather than cyclic opening and collapse. Crit. Care Med. 37, (2009). 3. Harding, E. M. & Robinson, R. J. Flow in a terminal alveolar sac model with expanding walls using computational fluid dynamics. Inhal. Toxicol. 22, (2010). 4. Semmler-Behnke, M. et al. Nanoparticle delivery in infant lungs. Proc. Natl Acad. Sci. USA 109, (2012). 5. Hwu, Y. et al. Synchrotron microangiography with no contrast agent. Phys. Med. Biol. 49, (2004). 6. Westneat, M. W., Socha, J. J. & Lee, W. K. Advances in biological structure, function, and physiology using synchrotron X-ray imaging, Annu. Rev. Physiol. 70, (2008). 7. Sera, T. et al. Development of high-resolution 4D in vivo-ct for visualization of cardiac and respiratory deformations of small animals, Phys. Med. Biol. 53, (2008). 8. Dubsky, S., Hooper, S. B., Siu, K. K. W. & Fouras, A. Synchrotron-based dynamic computed tomography of tissue motion for regional lung function measurement. J. R. Soc. Interface doi: /risf (2012)
GAS EXCHANGE IB TOPIC 6.4 CARDIOPULMONARY SYSTEM CARDIOPULMONARY SYSTEM. Terminal bronchiole Nasal cavity. Pharynx Left lung Alveoli.
IB TOPIC 6.4 GAS EXCHANGE CARDIOPULMONARY SYSTEM CARDIOPULMONARY SYSTEM Branch from the pulmonary artery (oxygen-poor blood) Branch from the pulmonary vein (oxygen-rich blood) Terminal bronchiole Nasal
More informationIB TOPIC 6.4 GAS EXCHANGE
IB TOPIC 6.4 GAS EXCHANGE CARDIOPULMONARY SYSTEM CARDIOPULMONARY SYSTEM Branch from the pulmonary artery (oxygen-poor blood) Branch from the pulmonary vein (oxygen-rich blood) Terminal bronchiole Nasal
More informationThe Respiratory System
13 PART A The Respiratory System PowerPoint Lecture Slide Presentation by Jerry L. Cook, Sam Houston University ESSENTIALS OF HUMAN ANATOMY & PHYSIOLOGY EIGHTH EDITION ELAINE N. MARIEB Organs of the Respiratory
More informationRespiratory System. Organization of the Respiratory System
Respiratory System In addition to the provision of oxygen and elimination of carbon dioxide, the respiratory system serves other functions, as listed in (Table 15 1). Respiration has two quite different
More informationDeposition of Inhaled Particle in the Human Lung for Different Age Groups
Deposition of Inhaled Particle in the Human Lung for Different Age Groups Xilong Guo 1, Qihong Deng 1* 1 Central South University (CSU), Changsha, China * Corresponding email: qhdeng@csu.edu.cn, qhdeng@gmail.com.
More informationThe Respiratory System
The Respiratory System If you have not done so already, please print and bring to class the Laboratory Practical II Preparation Guide. We will begin using this shortly in preparation of your second laboratory
More informationThe Pressure Losses in the Model of Human Lungs Michaela Chovancova, Pavel Niedoba
The Pressure Losses in the Model of Human Lungs Michaela Chovancova, Pavel Niedoba Abstract For the treatment of acute and chronic lung diseases it is preferred to deliver medicaments by inhalation. The
More informationRESPIRATORY SYSTEM. 1. Label the following parts of the respiratory system:
RSPIRTORY SYSTM 1. Label the following parts of the respiratory system: 2. In the diagram below, label the types of air volumes with the following terms: tidal volume, vital capacity, residual volume,
More informationsphere A diameter / cm 1 3 (i) The student calculated the surface area: volume ratio of sphere B as 2:1.
1. A student investigated how the surface area of a single-celled organism is related to its volume. The student used two spheres, A and B, as models of two organisms. The surface area and volume of each
More informationThree-Dimensional Quantitative Co-Mapping of Pulmonary Morphology and. Nanoparticle Distribution with Cellular Resolution in Nondissected Murine
Three-Dimensional Quantitative Co-Mapping of Pulmonary Morphology and Nanoparticle Distribution with Cellular Resolution in Nondissected Murine Lungs Lin Yang,,, Annette Feuchtinger, Winfried Möller,,
More information-Tamara Wahbeh. -Razan Abu Rumman. Dr. Mohammed Al-Muhtaseb
-2 -Tamara Wahbeh -Razan Abu Rumman Dr. Mohammed Al-Muhtaseb I tried to include everything the doctor mentioned in both the lecture and his slides in the simplest way possible, so hopefully there would
More informationSupplementary Materials for
advances.sciencemag.org/cgi/content/full/3/8/e1700521/dc1 Supplementary Materials for Functional vascularized lung grafts for lung bioengineering N. Valerio Dorrello, Brandon A. Guenthart, John D. O Neill,
More informationBioMedical quantitative X-Ray Imaging. Emmanuel Brun Researcher Inserm Université Grenoble Alpes
BioMedical quantitative X-Ray Imaging Emmanuel Brun Researcher Inserm Université Grenoble Alpes 1 Outline Introduction K-Edge Imaging Patient imaging at the European synchrotron Medical Phase Contrast
More informationRespiratory Physiology
Respiratory Physiology Dr. Aida Korish Associate Prof. Physiology KSU The main goal of respiration is to 1-Provide oxygen to tissues 2- Remove CO2 from the body. Respiratory system consists of: Passages
More informationChapter 11 The Respiratory System
Biology 12 Name: Respiratory System Per: Date: Chapter 11 The Respiratory System Complete using BC Biology 12, page 342-371 11.1 The Respiratory System pages 346-350 1. Distinguish between A. ventilation:
More informationTheta sequences are essential for internally generated hippocampal firing fields.
Theta sequences are essential for internally generated hippocampal firing fields. Yingxue Wang, Sandro Romani, Brian Lustig, Anthony Leonardo, Eva Pastalkova Supplementary Materials Supplementary Modeling
More informationLecture Overview. Respiratory System. Martini s Visual Anatomy and Physiology First Edition. Chapter 20 - Respiratory System Lecture 11
Martini s Visual Anatomy and Physiology First Edition Martini Ober Chapter 20 - Respiratory System Lecture 11 1 Lecture Overview Overview of respiration Functions of breathing Organs of the respiratory
More informationChapter 10. Respiratory System and Gas Exchange. Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Chapter 10 Respiratory System and Gas Exchange Function of the Respiratory System To obtain oxygen (O 2 ) for all cells in the body. To rid the cells of waste gas (CO 2 ). Oxygen (O 2 ) is vital chemical
More informationI. Anatomy of the Respiratory System A. Upper Respiratory System Structures 1. Nose a. External Nares (Nostrils) 1) Vestibule Stratified Squamous
I. Anatomy of the Respiratory System A. Upper Respiratory System Structures 1. Nose a. External Nares (Nostrils) 1) Vestibule Stratified Squamous Epithelium b. Nasal Cartilages 1) Nasal Cavity Pseudostratified
More informationNature Neuroscience doi: /nn Supplementary Figure 1. Characterization of viral injections.
Supplementary Figure 1 Characterization of viral injections. (a) Dorsal view of a mouse brain (dashed white outline) after receiving a large, unilateral thalamic injection (~100 nl); demonstrating that
More informationNature Neuroscience: doi: /nn Supplementary Figure 1. MADM labeling of thalamic clones.
Supplementary Figure 1 MADM labeling of thalamic clones. (a) Confocal images of an E12 Nestin-CreERT2;Ai9-tdTomato brain treated with TM at E10 and stained for BLBP (green), a radial glial progenitor-specific
More informationJohn Nguyen, Nozomi Nishimura, Robert Fetcho, Costantino Iadecola, Chris B. Schaffer
Supplemental figures and text for Occlusion of cortical ascending venules causes blood flow decreases, reversals in flow direction, and vessel dilation in upstream capillaries John Nguyen, Nozomi Nishimura,
More informationThe Influence of Altered Pulmonarv
The Influence of Altered Pulmonarv J Mechanics on the Adequacy of Controlled Ventilation Peter Hutchin, M.D., and Richard M. Peters, M.D. W ' hereas during spontaneous respiration the individual determines
More informationSupplementary Figure 1. Nature Neuroscience: doi: /nn.4547
Supplementary Figure 1 Characterization of the Microfetti mouse model. (a) Gating strategy for 8-color flow analysis of peripheral Ly-6C + monocytes from Microfetti mice 5-7 days after TAM treatment. Living
More informationSupplementary Figure 1 Information on transgenic mouse models and their recording and optogenetic equipment. (a) 108 (b-c) (d) (e) (f) (g)
Supplementary Figure 1 Information on transgenic mouse models and their recording and optogenetic equipment. (a) In four mice, cre-dependent expression of the hyperpolarizing opsin Arch in pyramidal cells
More informationRespiratory System. Chapter 9
Respiratory System Chapter 9 Air Intake Air in the atmosphere is mostly Nitrogen (78%) Only ~21% oxygen Carbon dioxide is less than 0.04% Air Intake Oxygen is required for Aerobic Cellular Respiration
More informationCD4 and CD8 T cells show a similar accumulation in the tumor stroma.
Fig S1 CD4 Fibronectin EpCM CD8 CD4 and CD8 T cells show a similar accumulation in the tumor stroma. Fluorescently-labeled CD4 (CMFD, green) and CD8 (Hoechst, yellow) T cells were added to a human lung
More informationEnergy is needed for cell activities: growth,reproduction, repair, movement, etc...
Respiration Energy is needed for cell activities: growth,reproduction, repair, movement, etc... Metabolism refers to all of the chemical reactions in the body, where molecules are synthesized (anabolism)
More informationmm Distance (mm)
b a Magnet Illumination Coverslips MPs Objective 2575 µm 1875 µm 1575 µm 1075 µm 875 µm 545 µm 20µm 2 3 0.5 0.3mm 1 1000 100 10 1 0.1 1000 100 10 1 0.1 Field Induction (Gauss) 1.5 0 5 10 15 20 Distance
More information4D PET: promises and limitations
4D PET: promises and limitations Tinsu Pan, Ph.D. M.D. Anderson Cancer Center The University of Texas Background Outlines Gating techniques: Deep inspiration breath hold 4D PET/CT Non-gating techniques
More informationChapter 10 The Respiratory System
Chapter 10 The Respiratory System Biology 2201 Why do we breathe? Cells carry out the reactions of cellular respiration in order to produce ATP. ATP is used by the cells for energy. All organisms need
More informationبسم هللا الرحمن الرحيم
بسم هللا الرحمن الرحيم Yesterday we spoke of the increased airway resistance and its two examples: 1) emphysema, where we have destruction of the alveolar wall and thus reducing the area available for
More informationRadiology of the respiratory disease
Radiology of the respiratory disease [ Color index: Important Notes Extra ] [ Editing file Feedback Share your notes Shared notes ] Resources: - 435 Slides - 434 Team - 435 Notes Done by: - Mai Alageel
More informationInfluenza virus exploits tunneling nanotubes for cell-to-cell spread
Supplementary Information Influenza virus exploits tunneling nanotubes for cell-to-cell spread Amrita Kumar 1, Jin Hyang Kim 1, Priya Ranjan 1, Maureen G. Metcalfe 2, Weiping Cao 1, Margarita Mishina 1,
More informationSUPPLEMENTARY INFORMATION
doi: 10.1038/nature06310 SUPPLEMENTARY INFORMATION www.nature.com/nature 1 www.nature.com/nature 2 www.nature.com/nature 3 Supplementary Figure S1 Spontaneous duration of wake, SWS and REM sleep (expressed
More informationThe promise of nebulized antibiotic therapy
1 st ATHENA International Conference Athens, 19-20 November 2015 Let s Talk About Inhaled Antibiotics Inhaled Antibiotics: The Story Stijn BLOT Dept. of Internal Medicine Faculty of Medicine & Health Science
More informationPULMONARY FUNCTION TESTS
Chapter 4 PULMONARY FUNCTION TESTS M.G.Rajanandh, Department of Pharmacy Practice, SRM College of Pharmacy, SRM University. OBJECTIVES Review basic pulmonary anatomy and physiology. Understand the reasons
More informationNature Structural & Molecular Biology: doi: /nsmb Supplementary Figure 1
Supplementary Figure 1 Design of isolated protein and RNC constructs, and homogeneity of purified RNCs. (a) Schematic depicting the design and nomenclature used for all the isolated proteins and RNCs used
More informationMonitor the patients disease pathology and response to therapy Estimate respiratory mechanics
Understanding Graphics during Mechanical Ventilation Why Understand Ventilator Graphics? Waveforms are the graphic representation of the data collected by the ventilator and reflect the interaction between
More informationCHAPTER 7.1 STRUCTURES OF THE RESPIRATORY SYSTEM
CHAPTER 7.1 STRUCTURES OF THE RESPIRATORY SYSTEM Pages 244-247 DO NOW What structures, do you think, are active participating in the breathing process? 2 WHAT ARE WE DOING IN TODAY S CLASS Finishing Digestion
More information? Pulmonary Respiratory System
The Structure of The Respiratory System The respiratory system is composed of groups of organelles that filters and transports air into the lungs. The organs comprising of the respiratory system include
More informationOrgans Histology D. Sahar AL-Sharqi. Respiratory system
Respiratory system The respiratory system provides for exchange of O2 and CO2 to and from the blood. Respiratory organs include the lungs and a branching system of bronchial tubes that link the sites of
More informationPrepared by : Bayan Kaddourah RN,MHM. GICU Clinical Instructor
Mechanical Ventilation Prepared by : Bayan Kaddourah RN,MHM. GICU Clinical Instructor 1 Definition Is a supportive therapy to facilitate gas exchange. Most ventilatory support requires an artificial airway.
More informationRESPIRATORY SYSTEM. A. Upper respiratory tract (Fig. 23.1) Use the half-head models.
RESPIRATORY SYSTEM I. OVERVIEW OF THE RESPIRATORY SYSTEM AND THORAX A. Upper respiratory tract (Fig. 23.1) Use the half-head models. Nasal cavity Pharynx (fare-rinks) B. Lower respiratory tract (Fig. 23.1)
More informationUnit 9. Respiratory System 16-1
Unit 9 Respiratory System 16-1 Works together with the circulatory system Exchange of gases between atmosphere, blood, and cells If respiratory system and/or circulatory system fails, death will occur
More informationMeasuring Exosome Stability with Nanoparticle Tracking Analysis
Measuring Exosome Stability with Nanoparticle Tracking Analysis PARTICLE CONCENTRATION PARTICLE SIZE Introduction The study of extracellular vesicles is an area that has become the subject of intense study
More informationMulti-modal anatomical Optical Coherence Tomography and CT for in vivo Dynamic Upper Airway Imaging
Multi-modal anatomical Optical Coherence Tomography and CT for in vivo Dynamic Upper Airway Imaging Santosh Balakrishnan a, Ruofei Bu a, Hillel Price b, Carlton Zdanski d, Amy L. Oldenburg a,b,c a Department
More informationCAPTAINS TRYOUTS 2018 ANATOMY & PHYSIOLOGY ANSWER KEY
CAPTAINS TRYOUTS 2018 ANATOMY & PHYSIOLOGY ANSWER KEY Section 1: Respiratory System (65 points) 1) It provides CO 2 and O 2 exchange, enables vocalization, provides the sense of smell, helps control the
More information7/12/2012. Respiratory system. Respiratory Response to Toxic Injury (Lung) Ninth Industrial Toxicology and Pathology Short Course.
Ninth Industrial Toxicology and Pathology Short Course 23 27 July, 2012 Contemporary Concepts in Target Organ Toxicologic Pathology Respiratory system Respiratory Response to Toxic Injury (Lung) Eric Wheeldon
More informationWenqin Hu, Cuiping Tian, Tun Li, Mingpo Yang, Han Hou & Yousheng Shu
Distinct contributions of Na v 1.6 and Na v 1.2 in action potential initiation and backpropagation Wenqin Hu, Cuiping Tian, Tun Li, Mingpo Yang, Han Hou & Yousheng Shu Supplementary figure and legend Supplementary
More informationSupplementary Figures
Supplementary Figures Supplementary Fig. 1. Current density profiles for backside-plating configuration cells and the cycle stability curve with and without carbon coating. Current density profiles of
More informationOxygenation. Chapter 45. Re'eda Almashagba 1
Oxygenation Chapter 45 Re'eda Almashagba 1 Respiratory Physiology Structure and function Breathing: inspiration, expiration Lung volumes and capacities Pulmonary circulation Respiratory gas exchange: oxygen,
More informationQuestions 1-3 refer to the following diagram. Indicate the plane labeled by the corresponding question number.
Name: Grade: ANATOMY TEST Questions 1-3 refer to the following diagram. Indicate the plane labeled by the corresponding question number. 1. Plane #1 is the... 2. Plane #2 is the... 3. Plane #3 is the...
More informationANATOMY AND BASIC FUNCTION OF THE ENDOCRINE GLANDS
ANATOMY AND BASIC FUNCTION OF THE ENDOCRINE GLANDS Know these endocrine organs of the cat: thymus, thyroid, pancreas, adrenal glands, ovaries, and testes. Review and know microslides, hormones, and structures
More informationThree-dimensional non-destructive soft-tissue visualization with X-ray staining micro-tomography - Supplementary Information
Three-dimensional non-destructive soft-tissue visualization with X-ray staining micro-tomography - Supplementary Information Juliana Martins de Souza e Silva 1,2, Irene Zanette 1,3, Peter B. Noël 1,4,
More informationNotes to complete gas exchange in mammals
Notes to complete gas exchange in mammals Mass flow of air to respiratory surface this is achieved through the mechanics of ventilation (breathing). This ensures a regular supply of air into and out of
More informationSupplementary information - Table (1), Figures (12), and Videos (5)
Supplementary information - Table (1), Figures (12), and Videos (5) A soft, transparent, freely accessible cranial window for chronic imaging and electrophysiology Chaejeong Heo 1, Hyejin Park 1, 2, Yong-Tae
More informationPotential conflicts-of-interest. Respiratory Gated and Four-Dimensional Tumor Tracking Radiotherapy. Educational objectives. Overview.
Respiratory Gated and Four-Dimensional Tumor Tracking Radiotherapy Potential conflicts-of-interest I am PI of a sponsored research agreement between Stanford University and Varian Medical Systems P Keall
More informationRespiration Lesson 3. Respiration Lesson 3
Respiration Lesson 3 and Airway Resistance (key factors affecting air flow) 1) What is the arterial blood pressure in a healthy 18 year old male? 2) What would his venous blood pressure be? 3) What is
More informationSUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb2988 Supplementary Figure 1 Kif7 L130P encodes a stable protein that does not localize to cilia tips. (a) Immunoblot with KIF7 antibody in cell lysates of wild-type, Kif7 L130P and Kif7
More informationRespiratory System Functions. Respiratory System Organization. Respiratory System Organization
Respiratory System Functions Functions of Respiratory System Gas exchange between blood and air Move air to and from exchange surfaces Protect exchange surfaces from environmental variations and pathogens
More informationSupplementary table and figures
3D single molecule tracking with multifocal plane microscopy reveals rapid intercellular transferrin transport at epithelial cell barriers Sripad Ram, Dongyoung Kim, Raimund J. Ober and E. Sally Ward Supplementary
More informationFunctional Respiratory Imaging
Functional Respiratory Imaging (FRI) to assess the bioequivalence of inhaled medication Jan De Backer, MSc, PhD, MBA CEO BABE 2014 Functional Respiratory Imaging (FRI) FRI outcome parameters Added value
More informationParticle Size and Dust Inhalation
Pneumoconiosis A disease of the lungs characterized by fibrosis and caused by the chronic inhalation of mineral dusts, especially silica and asbestos. Helen Lang Dept. Geology & Geography West Virginia
More informationMicrotubule Teardrop Patterns
Supporting Information Microtubule Teardrop Patterns Kosuke Okeyoshi 1, Ryuzo Kawamura 1, Ryo Yoshida 2, and Yoshihito Osada 1 * 1 RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako-shi, Saitama 351-0198,
More informationRESPIRATORY PHYSIOLOGY Pre-Lab Guide
RESPIRATORY PHYSIOLOGY Pre-Lab Guide NOTE: A very useful Study Guide! This Pre-lab guide takes you through the important concepts that where discussed in the lab videos. There will be some conceptual questions
More informationRespiratory Physiology In-Lab Guide
Respiratory Physiology In-Lab Guide Read Me Study Guide Check Your Knowledge, before the Practical: 1. Understand the relationship between volume and pressure. Understand the three respiratory pressures
More informationAnatomy & Physiology 2 Canale. Respiratory System: Exchange of Gases
Anatomy & Physiology 2 Canale Respiratory System: Exchange of Gases Why is it so hard to hold your breath for Discuss! : ) a long time? Every year carbon monoxide poisoning kills 500 people and sends another
More informationa. dissolved gases b. nutrients c. globulins d. surfactants e. hormones
Example Lecture Test: Blood, Respiratory Multiple Choice. Read each question thoroughly before answering. From the choices available, choose the answer that is the most correct. Place all answers on the
More informationNature Neuroscience: doi: /nn Supplementary Figure 1. Confirmation that optogenetic inhibition of dopaminergic neurons affects choice
Supplementary Figure 1 Confirmation that optogenetic inhibition of dopaminergic neurons affects choice (a) Sample behavioral trace as in Figure 1d, but with NpHR stimulation trials depicted as green blocks
More informationMotion gating and tracking techniques: overview and recent developments
Motion gating and tracking techniques: overview and recent developments Gig S Mageras, PhD, FAAPM Department of Medical Physics Memorial Sloan Kettering Cancer Center, New York MSK/gsm 15-Jun-2018 1 Disclosure
More informationSupporting Information for
Supporting Information for Rupture of Lipid Membranes Induced by Amphiphilic Janus Nanoparticles Kwahun Lee, Liuyang Zhang, Yi Yi, Xianqiao Wang, Yan Yu* Department of Chemistry, Indiana University, Bloomington,
More informationThe respiratory system
The respiratory system Practical 1 Objectives Respiration, ventilation Intrapleural and intrapulmonary pressure Mechanism of inspiration and expiration Composition of the atmosphere and the expired air
More informationPatient. Device Clinician. Safety & efficacy
Patient Device Clinician Formulation Safety & efficacy 1. Modified from Daley-Yates et al., Expert Opin. Drug Deliv. 2011: 8(10):1297-1308 2. Modified from Laube et al., Eur Respir J 2011; 37: 1308 1331
More informationAirway pressure release ventilation (APRV) in PICU: Current evidence. Chor Yek Kee Sarawak General Hospital
Airway pressure release ventilation (APRV) in PICU: Current evidence Chor Yek Kee Sarawak General Hospital Outline Brief introduction of APRV History of APRV Common confusion in APRV Features of APRV and
More informationNURSE-UP RESPIRATORY SYSTEM
NURSE-UP RESPIRATORY SYSTEM FUNCTIONS OF THE RESPIRATORY SYSTEM Pulmonary Ventilation - Breathing Gas exchanger External Respiration between lungs and bloodstream Internal Respiration between bloodstream
More informationThe RESPIRATORY System. Unit 9
The RESPIRATORY System Unit 9 Respiration The exchange of gases between the atmosphere, blood, and cells Pulmonary Ventilation - the exchange of air between the atmosphere and lungs External (Pulmonary)
More informationNature Neuroscience: doi: /nn Supplementary Figure 1. Distribution of starter cells for RV-mediated retrograde tracing.
Supplementary Figure 1 Distribution of starter cells for RV-mediated retrograde tracing. Parcellation of cortical areas is based on Allen Mouse Brain Atlas and drawn to scale. Thick white curves, outlines
More informationKarel Roubík, Vladimír Sobota
Electrical Impedance Tomography for lung recruitment monitoring Technical aspects of clinical usage and interpretation Karel Roubík, Vladimír Sobota Faculty of Biomedical Engineering Czech Technical University
More informationNAME PER DATE. membrane
NAME PER DATE Chapter 9, Section 1 Review Matching: 1. alveolar capillary membrane 2. alveoli 3. bronchioles 4. cardiopulmonary system 5. conchae 6. epiglottis 7. larynx 8. mediastinum 9. nares 10. olfactory
More information1. When a patient fails to ventilate or oxygenate adequately, the problem is caused by pathophysiological factors such as hyperventilation.
Chapter 1: Principles of Mechanical Ventilation TRUE/FALSE 1. When a patient fails to ventilate or oxygenate adequately, the problem is caused by pathophysiological factors such as hyperventilation. F
More informationTanimoto et al., http ://www.jcb.org /cgi /content /full /jcb /DC1
Supplemental material JCB Tanimoto et al., http ://www.jcb.org /cgi /content /full /jcb.201510064 /DC1 THE JOURNAL OF CELL BIOLOGY Figure S1. Method for aster 3D tracking, extended characterization of
More informationUnderstanding the mode of action of a drug using Functional Respiratory Imaging (FRI) Roflumilast Study. Jan De Backer, MSc, PhD, MBA CEO
Understanding the mode of action of a drug using Functional Respiratory Imaging (FRI) Roflumilast Study Jan De Backer, MSc, PhD, MBA CEO IPAC RS/UF Orlando Inhalation Conference March 20, 2014 OVERVIEW
More informationFile name: Supplementary Information Description: Supplementary Figures, Supplementary Table and Supplementary References
File name: Supplementary Information Description: Supplementary Figures, Supplementary Table and Supplementary References File name: Supplementary Data 1 Description: Summary datasheets showing the spatial
More informationLesson 9.1: Learning the Key Terms
131 Lesson 9.1: Learning the Key Terms Directions: Place the letter of the best definition next to each key term. 1. alveolar capillary membrane 2. alveoli 3. bronchioles 4. cardiopulmonary system 5. conchae
More informationSupplementary Figures
Supplementary Figures Supplementary Figure 1: Predicted structure of the most stable {110} antiphase boundary defect in magnetite (model APB-I). a) The same structure as that shown in Fig. 1b (main text)
More informationHigh resolution structural evidence suggests the Sarcoplasmic Reticulum forms microdomains with Acidic Stores (lyososomes) in the heart.
High resolution structural evidence suggests the Sarcoplasmic Reticulum forms microdomains with Acidic Stores (lyososomes) in the heart. Daniel Aston, Rebecca A. Capel, Kerrie L. Ford, Helen C. Christian,
More informationHDL surface lipids mediate CETP binding as revealed by electron microscopy and molecular dynamics simulation
HDL surface lipids mediate CETP binding as revealed by electron microscopy and molecular dynamics simulation Meng Zhang 1, River Charles 1, Huimin Tong 1, Lei Zhang 1, Mili Patel 2, Francis Wang 1, Matthew
More informationTransactions on Biomedicine and Health vol 2, 1995 WIT Press, ISSN
Biomedical application of the supercomputer: targeted delivery of inhaled Pharmaceuticals in diseased lungs T.B. Martonen,* I. Katz,* D. Hwang,' Y.Yang* "Health Effects Research Laboratory, U.S.Environmental
More informationAnalisi avanzata dello washout di gas inerti
Analisi avanzata dello washout di gas inerti (relation to models & imaging) Sylvia Verbanck Volume (L) Flow (L/s) R, X (kpa.l -.s) volume (L) volume (L) Non-invasive lung function testing spirometry impedance
More informationSpray Nebulizer Deposition Efficiency as a Function of Age. University of Denver Denver, CO Denver, CO
ILASS Americas, 23 rd Annual Conference on Liquid Atomization and Spray Systems, Ventura, CA, May 2011 Spray Nebulizer Deposition Efficiency as a Function of Age L. Weber * and C.S. Lengsfeld 1 Department
More informationNature Neuroscience: doi: /nn Supplementary Figure 1
Supplementary Figure 1 Reward rate affects the decision to begin work. (a) Latency distributions are bimodal, and depend on reward rate. Very short latencies (early peak) preferentially occur when a greater
More informationd e f Spatiotemporal quantification of subcellular ATP levels in a single HeLa cell during changes in morphology Supplementary Information
Ca 2+ level (a. u.) Area (a. u.) Normalized distance Normalized distance Center Edge Center Edge Relative ATP level Relative ATP level Supplementary Information Spatiotemporal quantification of subcellular
More informationThe respiratory system structure and function
Name: Class: Date: Active reading 11A + Biology Gr11A The respiratory system structure and function The function of the respiratory system is to bring oxygen into the body and eliminate carbon dioxide
More informationThe Respiratory System Structures of the Respiratory System Structures of the Respiratory System Structures of the Respiratory System Nose Sinuses
CH 14 D.E. Human Biology The Respiratory System The Respiratory System OUTLINE: Mechanism of Breathing Transport of Gases between the Lungs and the Cells Respiratory Centers in the Brain Function Provides
More informationSUPPORTING INFORMATION. Lysine Carbonylation is a Previously Unrecognized Contributor. to Peroxidase Activation of Cytochrome c by Chloramine-T
Electronic Supplementary Material (ESI) for Chemical Science. This journal is The Royal Society of Chemistry 2019 SUPPORTING INFORMATION Lysine Carbonylation is a Previously Unrecognized Contributor to
More informationNature Neuroscience: doi: /nn Supplementary Figure 1. Behavioral training.
Supplementary Figure 1 Behavioral training. a, Mazes used for behavioral training. Asterisks indicate reward location. Only some example mazes are shown (for example, right choice and not left choice maze
More informationThe Respiratory System. Dr. Ali Ebneshahidi
The Respiratory System Dr. Ali Ebneshahidi Functions of The Respiratory System To allow gases from the environment to enter the bronchial tree through inspiration by expanding the thoracic volume. To allow
More informationChapter 16. Respiratory System
Chapter 16 Respiratory System Introduction Respiration = the entire process of exchanging gases between the atmosphere and body cells 1. Ventilation 2. Gas exchange 3. Gas transport : 4. Cellular respiration
More informationSESSION IV: MECHANISMS OF HUMAN DISEASE: LABORATORY SESSIONS PULMONARY PATHOLOGY I. December 5, 2012
SESSION IV: MECHANISMS OF HUMAN DISEASE: LABORATORY SESSIONS PULMONARY PATHOLOGY I December 5, 2012 FACULTY COPY GOAL: Describe the basic morphologic and pathophysiologic changes in various conditions
More information