Lecture 3-4. Identification of Positive Regulators downstream of SA
|
|
- Sharyl Pope
- 5 years ago
- Views:
Transcription
1 Lecture 3-4 Identification of Positive Regulators downstream of SA
2 Positive regulators between SA and resistance/pr Systemic resistance PR gene expression? SA Local infection Necrosis SA What could they be?
3 Hunt for regulators of SAR Genetic screens for positive regulators downstream of SA How do you find mutants non-responsive to SA?
4 Hunt for regulators of SAR Case 1: John Ryals Group Host plant: Arabidopsis Ws ecotype Pathogen: oomycete Peronospora parasitica Emwa2, causes downy mildew Treatment: INA or SA spray followed by Emwa2 inoculation 280,000 M2 plants from EMS mutagenesis and 80,000 T4 plants (from T-DNA transformation) identified 75 lines that were defective in SAR. nim1 compromised the most.
5 WT nim1 H 2 O INA SA
6 Where to place NIM1?
7 NIM1 Systemic resistance PR gene expression SA? Local infection Necrosis? SA
8 SA SA UDP-Glycosyltransferase SAG (SA+SAG) What does the figure tell us about NIM1 function?
9 Systemic resistance PR gene expression NIM1 SA? Necrosis SA Local infection
10 What genetic tests need to be done with nim1? - Heritable? - Dominant/recessive? - Single gene? - Allelism test
11 nim1 x WT Conclusions?
12 Conclusions?
13 Conclusions?
14 Induction of PR-1 mrna by pathogen infection or chemicals Pathogens? SA?? NIM1 nim1-5 PR1 PR1? - Not allelic to nim1-1 - unusual allele of nim1 - Allelic to nim1-1, other mutations affect the phenotype
15 How would you clone nim1? nim1-1 x Ler F1 F2 Crude mapping fine mapping
16 Crude mapping Fine mapping Cosmid contig
17 Which clones contain the NIM1 gene?
18 Sequenced the overlapping region and a mutation found in nim1-1
19 Is nim1 cloned? What else should we do? - Sequence other alleles - Single gene complementation - Test T-DNA knockout alleles
20
21
22 nim1-5 - Not allelic to nim1-1 - unusual allele of nim1 - Allelic to nim1-1, other mutations affect the phenotype? Which agrees with the sequencing data?
23 Systemic resistance PR gene expression NIM1 SA? Necrosis SA Local infection
24 Hunt for regulators of SAR Forward genetic screens conducted to search for positive regulators downstream of SA Case 2: Xinnian Dong s Group at Duke University Screen for mutants in which PR genes can not be induced by SA How?
25 BGL2 (PR2) Promoter-GUS reporter BGL2 promoter GUS Pathogen SA or INA GUS: β-glucuronidase
26 WT SA or INA npr1-1 SA or INA EMS, 13,500 M2 plants screened, 76 putative mutants identified npr: non-expresser of PR genes
27 What kinds of mutations can abolish PR2 promoter-gus expression? - Genes required for induction of PR2 by SA - GUS reporter gene
28 How would you confirm your mutants? Systemic resistance PR gene expression SA? Necrosis SA Local infection
29 npr1-1
30
31 NPR1 Systemic resistance PR gene expression SA? Necrosis SA Local infection
32 Map of the NPR1 Locus on Chromosome I 1 2 3
33 H 2 O INA SA Clone 1 Clone 2 Clone 3 WT npr1-2
34 Map of the NPR1 Locus on Chromosome I 1 2 3
35 Summary of positional cloning Generate a mapping population Pick plants with the mutant phenotype in F2 Crude mapping Generate additional markers Fine mapping of the mutation Generate cosmid contigs Perform complementation analysis Sequence overlapping region to identify the mutation PCR Seq Whole genome Seq
36 Predict the function of NPR1
37 Predict the function of NPR1 1. Blast search 2. Identification of protein domains SMART (Simple Modular Architecture Research Tool) 3. Prediction of protein subcellular localization PSORT, WoLF PSORT 4. Analysis of gene expression pattern Arabidopsis efp Browser Where were the data used to build the efp Browser coming from?
38 NPR1 is a protein with ankyrin repeats What does it tell us about how NPR1 functions?
39 NIM1/NPR1 Systemic resistance PR gene expression SA? Necrosis SA Local infection
40 Forward genetic screens conducted to search for positive regulators downstream of SA Lab Screens Mutants obtained Ryals, Novartis Non-immunity (NIM) nim1-1 to nim1-6 Klessig, Rutgers Dong, Duke Ausubel, Harvard SA insensitive (SAI) No PR-gene expression (NPR) Enhanced disease susceptibility (EDS) sai1-1 and sai1-2 npr1-1 npr1-2 to npr1-4
41 Why all the SAR screens only yielded mutants carrying mutations in one gene --- NPR1? Why other things such as TF were not found?
42 NPR1 is a positive regulator of SAR. Can we use NPR1 to regulate the SAR pathway? How would you enhance SAR with NPR1?
43
44 Disease symptoms caused by Psm ES4326
45
46
47
48
49 Growth of the oomycete pathogen Peronospora parasitica Noco2 on wild type and NPR1 transgenic lines
50 What can we conclude from the data?
51 35S-NPR1-H snc1 WT
52 References: 1. Buchanan et al Biochemistry and molecular biology of plants. ASPP, Rockville, MD. Chapter Cao H, Bowling SA, Gordon AS, Dong X. Characterization of an Arabidopsis Mutant That Is Nonresponsive to Inducers of Systemic Acquired Resistance. Plant Cell Nov;6(11): Cao H, Glazebrook J, Clarke JD, Volko S, Dong X. The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats. Cell ;88(1): Cao H, Li X, Dong X. Generation of broad-spectrum disease resistance by overexpression of an essential regulatory gene in systemic acquired resistance. Proc Natl Acad Sci U S A May 26;95(11): Delaney TP, Friedrich L, Ryals JA. Arabidopsis signal transduction mutant defective in chemically and biologically induced disease resistance. Proc Natl Acad Sci U S A Jul 3;92(14): Ryals J, Weymann K, Lawton K, Friedrich L, Ellis D, Steiner HY, Johnson J, Delaney TP, Jesse T, Vos P, Uknes S. The Arabidopsis NIM1 protein shows homology to the mammalian transcription factor inhibitor I kappa B. Plant Cell Mar;9(3):
Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene
Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene YUELIN ZHANG, WEIHUA FAN, MARK KINKEMA, XIN LI, AND
More informationSUPPLEMENTARY INFORMATION
Supplementary igure 1. The expression level of PP4 (At4g16860) is diminished in the rpp4 mutant (SK017569). The mna was extracted to analyze the expression level of PP4 using quantitative PC (qpc). Gene
More informationTesting the ABC floral-organ identity model: expression of A and C function genes
Objectives: Testing the ABC floral-organ identity model: expression of A and C function genes To test the validity of the ABC model for floral organ identity we will: 1. Use the model to make predictions
More informationBIOL2005 WORKSHEET 2008
BIOL2005 WORKSHEET 2008 Answer all 6 questions in the space provided using additional sheets where necessary. Hand your completed answers in to the Biology office by 3 p.m. Friday 8th February. 1. Your
More informationDivision of Biology and Molecular, Cellular, and Developmental Biology Program, Kansas State University, Manhattan, Kansas
The Plant Cell, Vol. 16, 465 477, February 2004, www.plantcell.org ª 2004 American Society of Plant Biologists The Arabidopsis thaliana Dihydroxyacetone Phosphate Reductase Gene SUPPRESSOR OF FATTY ACID
More informationRegulation of Floral-Organ- Type by SUPERMAN
Regulation of Floral-Organ- Type by SUPERMAN 1. Need for regulators of the organ-identity genes. 2. The Superman mutant phenotype-predicting the role of SUPERMAN. 3. Testing our hypothesis of the role
More informationHands-On Ten The BRCA1 Gene and Protein
Hands-On Ten The BRCA1 Gene and Protein Objective: To review transcription, translation, reading frames, mutations, and reading files from GenBank, and to review some of the bioinformatics tools, such
More informationThe Plant Cell, Vol. 15, , December 2003, American Society of Plant Biologists
, Vol. 15, 2952 2965, December 2003, www.plantcell.org 2003 American Society of Plant Biologists Plastidial Fatty Acid Signaling Modulates Salicylic Acid and Jasmonic Acid Mediated Defense Pathways in
More informationMolecular, Cellular, and Developmental Biology Program, Kansas State University, Manhattan, Kansas c
This article is published in Online, Preview Section, which publishes manuscripts accepted for publication after they have been edited and the authors have corrected proofs, but before the final, complete
More informationJyoti Shah, Vamsi Nalam, Sujon Sarowar, Syeda Alam, Sumita Behera, Hyeonju Lee, Delia Burdan and Harold N. Trick
Targeting Host Defense and Susceptibility Mechanisms for Engineering FHB Resistance Jyoti Shah, Vamsi Nalam, Sujon Sarowar, Syeda Alam, Sumita Behera, Hyeonju Lee, Delia Burdan and Harold N. Trick General
More informationSupplementary Figure 1 Transcription assay of nine ABA-responsive PP2C. Transcription assay of nine ABA-responsive PP2C genes. Total RNA was isolated
Supplementary Figure 1 Transcription assay of nine ABA-responsive PP2C genes. Transcription assay of nine ABA-responsive PP2C genes. Total RNA was isolated from 7 day-old seedlings treated with or without
More informationFunctional analysis of plant disease resistance genes and their downstream effectors Gregory B Martin
273 Functional analysis of plant disease resistance genes and their downstream effectors Gregory B Martin Plant disease resistance (R) genes encode proteins that both determine recognition of specific
More informationSupplemental Figure 1. Genes showing ectopic H3K9 dimethylation in this study are DNA hypermethylated in Lister et al. study.
mc mc mc mc SUP mc mc Supplemental Figure. Genes showing ectopic HK9 dimethylation in this study are DNA hypermethylated in Lister et al. study. Representative views of genes that gain HK9m marks in their
More informationTITLE: A Mouse Model to Investigate the Role of DBC2 in Breast Cancer
AD Award Number: W81XWH-04-1-0325 TITLE: A Mouse Model to Investigate the Role of DBC2 in Breast Cancer PRINCIPAL INVESTIGATOR: Valerie Boka CONTRACTING ORGANIZATION: University of Texas Health Science
More informationEarly cell death (FGF) B No RunX transcription factor produced Yes No differentiation
Solution Key - Practice Questions Question 1 a) A recent publication has shown that the fat stem cells (FSC) can act as bone stem cells to repair cavities in the skull, when transplanted into immuno-compromised
More informationDEPARTMENT OF BIOCHEMISTRY & MOLECULAR BIOLOGY
DEPARTMENT OF BIOCHEMISTRY & MOLECULAR BIOLOGY UNDERGRADUATE POSTER SESSION Monday, April 11, 2011 2:00-4:00pm Lisa Pinkava Under the Direction of Dr. Michael Thomashow Plant Research Laboratory Title:
More informationDarwin s dilemma 8/14/2014. Meiosis & Inheritance Lecture 18 Summer Mitosis & Meiosis. The Modern Synthesis
Darwin s dilemma 1 Meiosis & Inheritance Lecture 18 Summer 2014 How do organisms pass heritable traits to their offspring? The Modern Synthesis 2 Mitosis & Meiosis 3 1844 - Darwin essay on Natural Selection
More informationCancer. The fundamental defect is. unregulated cell division. Properties of Cancerous Cells. Causes of Cancer. Altered growth and proliferation
Cancer The fundamental defect is unregulated cell division. Properties of Cancerous Cells Altered growth and proliferation Loss of growth factor dependence Loss of contact inhibition Immortalization Alterated
More informationAnalysis of Massively Parallel Sequencing Data Application of Illumina Sequencing to the Genetics of Human Cancers
Analysis of Massively Parallel Sequencing Data Application of Illumina Sequencing to the Genetics of Human Cancers Gordon Blackshields Senior Bioinformatician Source BioScience 1 To Cancer Genetics Studies
More informationUNIT 3 GENETICS LESSON #30: TRAITS, GENES, & ALLELES. Many things come in many forms. Give me an example of something that comes in many forms.
UNIT 3 GENETICS LESSON #30: TRAITS, GENES, & ALLELES Many things come in many forms. Give me an example of something that comes in many forms. Genes, too, come in many forms. Main Idea #1 The same gene
More informationMultistep nature of cancer development. Cancer genes
Multistep nature of cancer development Phenotypic progression loss of control over cell growth/death (neoplasm) invasiveness (carcinoma) distal spread (metastatic tumor) Genetic progression multiple genetic
More informationChapter 4. Salicylic acid accumulation in transgenic Brassica rapa transformed with a gene encoding bacterial isochorismate synthase
Chapter 4 Salicylic acid accumulation in transgenic Brassica rapa transformed with a gene encoding bacterial isochorismate synthase Sanimah Simoh 1,2, Natali R Mustafa 1, Huub JM Linthorst 3, Robert Verpoorte
More informationThe Chromosomal Basis Of Inheritance
The Chromosomal Basis Of Inheritance Chapter 15 Objectives Explain the chromosomal theory of inheritance and its discovery. Explain why sex-linked diseases are more common in human males than females.
More informationChapter 15: The Chromosomal Basis of Inheritance
Name Period Chapter 15: The Chromosomal Basis of Inheritance Concept 15.1 Mendelian inheritance has its physical basis in the behavior of chromosomes 1. What is the chromosome theory of inheritance? 2.
More informationFor a long time, people have observed that offspring look like their parents.
Chapter 10 For a long time, people have observed that offspring look like their parents. Even before we knew about genes, people were breeding livestock to get certain traits in the offspring. They knew
More informationCancer Genetics. What is Cancer? Cancer Classification. Medical Genetics. Uncontrolled growth of cells. Not all tumors are cancerous
Session8 Medical Genetics Cancer Genetics J avad Jamshidi F a s a U n i v e r s i t y o f M e d i c a l S c i e n c e s, N o v e m b e r 2 0 1 7 What is Cancer? Uncontrolled growth of cells Not all tumors
More informationISSN: Bogatzevska & Stoyanova J. BioSci. Biotechnol. 2015, SE/ONLINE: RESEARCH ARTICLE. Nevena Bogatzevska Mariya Stoyanova
Nevena Bogatzevska Mariya Stoyanova Induction of systemic acquired resistance in pepper to X. vesicatoria pepper pathotype after treatment with X. vesicatoria tomato pathotype and Pseudomonas syringae
More informationCancer. The fundamental defect is. unregulated cell division. Properties of Cancerous Cells. Causes of Cancer. Altered growth and proliferation
Cancer The fundamental defect is unregulated cell division. Properties of Cancerous Cells Altered growth and proliferation Loss of growth factor dependence Loss of contact inhibition Immortalization Alterated
More informationCANCER. Inherited Cancer Syndromes. Affects 25% of US population. Kills 19% of US population (2nd largest killer after heart disease)
CANCER Affects 25% of US population Kills 19% of US population (2nd largest killer after heart disease) NOT one disease but 200-300 different defects Etiologic Factors In Cancer: Relative contributions
More informationFloral Organ Mutants and the Study of Organ Morphogenesis
Floral Organ Mutants and the Study of Organ Morphogenesis Objectives: 1. How does one use mutants to understand floral organ morphogenesis? 2. What are the phenotypes of some floral organ mutants? 3. What
More informationSMALL GROUP PROBLEM SOLVING SESSION GENETICS
SMALL GOU OBLEM SOLVING SESSION GENETICS DATE: Thursday, August 28, 2014 8:30am 10:30 am LEANING GOAL By attending and participating in this Small Group roblem Solving Session, you will be able to describe
More informationLecture 7. Chapter 5: Extensions and Modifications of Basic Principles, Part 2. Complementation Test. white squash x white squash WwYy x WwYy
Lecture 7 white squash x white squash WwYy x WwYy Chapter 5: Extensions and Modifications of Basic Principles, Part 2 Problem Set 1B due on Monday Genotype W_Y_ 9/16 W_yy 3/16 wwy_ 3/16 wwyy 1/16 Phenotype
More informationSupplementary Figure 1. Schematic diagram of o2n-seq. Double-stranded DNA was sheared, end-repaired, and underwent A-tailing by standard protocols.
Supplementary Figure 1. Schematic diagram of o2n-seq. Double-stranded DNA was sheared, end-repaired, and underwent A-tailing by standard protocols. A-tailed DNA was ligated to T-tailed dutp adapters, circularized
More informationChapter 15: The Chromosomal Basis of Inheritance
Name Chapter 15: The Chromosomal Basis of Inheritance 15.1 Mendelian inheritance has its physical basis in the behavior of chromosomes 1. What is the chromosome theory of inheritance? 2. Explain the law
More informationProgrammed Cell Death (apoptosis)
Programmed Cell Death (apoptosis) Stereotypic death process includes: membrane blebbing nuclear fragmentation chromatin condensation and DNA framentation loss of mitochondrial integrity and release of
More informationControl of Cell Proliferation by Peptide Growth Factors. Autocrine Growth Factor Production Causes Malignant Transformation?
Control of Cell Proliferation by Peptide Growth Factors Autocrine Growth Factor Production Causes Malignant Transformation? Transforming Activities From Condition Media from a Tumor Cell Line Condition
More informationInsulin Resistance. Biol 405 Molecular Medicine
Insulin Resistance Biol 405 Molecular Medicine Insulin resistance: a subnormal biological response to insulin. Defects of either insulin secretion or insulin action can cause diabetes mellitus. Insulin-dependent
More informationOleic acid levels regulated by glycerolipid metabolism modulate defense gene expression in Arabidopsis
Oleic acid levels regulated by glycerolipid metabolism modulate defense gene expression in Arabidopsis Aardra Kachroo*, Srivathsa C. Venugopal*, Ludmila Lapchyk*, Deane Falcone, David Hildebrand, and Pradeep
More informationHigh coverage in planta RNA sequencing identifies Fusarium oxysporum effectors and Medicago truncatularesistancemechanisms
High coverage in planta RNA sequencing identifies Fusarium oxysporum effectors and Medicago truncatularesistancemechanisms Louise Thatcher Gagan Garg, Angela Williams, Judith Lichtenzveig and Karam Singh
More informationSUPPLEMENTARY INFORMATION
doi:10.1038/nature10643 Supplementary Table 1. Identification of hecw-1 coding polymorphisms at amino acid positions 322 and 325 in 162 strains of C. elegans. WWW.NATURE.COM/NATURE 1 Supplementary Figure
More informationProblem set questions from Final Exam Human Genetics, Nondisjunction, and Cancer
Problem set questions from Final Exam Human Genetics, Nondisjunction, and ancer Mapping in humans using SSRs and LOD scores 1. You set out to genetically map the locus for color blindness with respect
More informationThe Biology and Genetics of Cells and Organisms The Biology of Cancer
The Biology and Genetics of Cells and Organisms The Biology of Cancer Mendel and Genetics How many distinct genes are present in the genomes of mammals? - 21,000 for human. - Genetic information is carried
More information3. What law of heredity explains that traits, like texture and color, are inherited independently of each other?
Section 2: Genetics Chapter 11 pg. 308-329 Part 1: Refer to the table of pea plant traits on the right. Then complete the table on the left by filling in the missing information for each cross. 6. What
More informationLaboratory. Mendelian Genetics
Laboratory 9 Mendelian Genetics Biology 171L FA17 Lab 9: Mendelian Genetics Student Learning Outcomes 1. Predict the phenotypic and genotypic ratios of a monohybrid cross. 2. Determine whether a gene is
More informationSUPPLEMENTARY INFORMATION
doi:10.1038/nature10495 WWW.NATURE.COM/NATURE 1 2 WWW.NATURE.COM/NATURE WWW.NATURE.COM/NATURE 3 4 WWW.NATURE.COM/NATURE WWW.NATURE.COM/NATURE 5 6 WWW.NATURE.COM/NATURE WWW.NATURE.COM/NATURE 7 8 WWW.NATURE.COM/NATURE
More informationIntroduction to Cancer Biology
Introduction to Cancer Biology Robin Hesketh Multiple choice questions (choose the one correct answer from the five choices) Which ONE of the following is a tumour suppressor? a. AKT b. APC c. BCL2 d.
More information8 Suppression Analysis
Genetic Techniques for Biological Research Corinne A. Michels Copyright q 2002 John Wiley & Sons, Ltd ISBNs: 0-471-89921-6 (Hardback); 0-470-84662-3 (Electronic) 8 Suppression Analysis OVERVIEW Suppression
More informationGenome 371, Autumn 2018 Quiz Section 9: Genetics of Cancer Worksheet
Genome 371, Autumn 2018 Quiz Section 9: Genetics of Cancer Worksheet All cancer is due to genetic mutations. However, in cancer that clusters in families (familial cancer) at least one of these mutations
More informationBoosting lipid productivity of the oleaginous microalga N. gaditana via genome engineering
Boosting lipid productivity of the oleaginous microalga N. gaditana via genome engineering Imad Ajjawi, John Verruto, Moena Aqui, Eric Moellering and Robert Brown October 25th 216 Outline n Brief intro
More informationFatty Acid Desaturation
Fatty Acid Desaturation Objectives: 1. Isolation of desaturase mutants 2. Substrates for fatty acid desaturation 3. ellular localization of desaturases References: Buchanan et al. 2000. Biochemistry and
More informationSrc-INACTIVE / Src-INACTIVE
Biology 169 -- Exam 1 February 2003 Answer each question, noting carefully the instructions for each. Repeat- Read the instructions for each question before answering!!! Be as specific as possible in each
More informationComparison of open chromatin regions between dentate granule cells and other tissues and neural cell types.
Supplementary Figure 1 Comparison of open chromatin regions between dentate granule cells and other tissues and neural cell types. (a) Pearson correlation heatmap among open chromatin profiles of different
More informationA gene is a sequence of DNA that resides at a particular site on a chromosome the locus (plural loci). Genetic linkage of genes on a single
8.3 A gene is a sequence of DNA that resides at a particular site on a chromosome the locus (plural loci). Genetic linkage of genes on a single chromosome can alter their pattern of inheritance from those
More informationProblem Set 8 Key 1 of 8
7.06 2003 Problem Set 8 Key 1 of 8 7.06 2003 Problem Set 8 Key 1. As a bright MD/PhD, you are interested in questions about the control of cell number in the body. Recently, you've seen three patients
More informationProblem Set 5 KEY
2006 7.012 Problem Set 5 KEY ** Due before 5 PM on THURSDAY, November 9, 2006. ** Turn answers in to the box outside of 68-120. PLEASE WRITE YOUR ANSWERS ON THIS PRINTOUT. 1. You are studying the development
More informationp53 and Apoptosis: Master Guardian and Executioner Part 2
p53 and Apoptosis: Master Guardian and Executioner Part 2 p14arf in human cells is a antagonist of Mdm2. The expression of ARF causes a rapid increase in p53 levels, so what would you suggest?.. The enemy
More informationEarly Embryonic Development
Early Embryonic Development Maternal effect gene products set the stage by controlling the expression of the first embryonic genes. 1. Transcription factors 2. Receptors 3. Regulatory proteins Maternal
More informationBreast cancer. Risk factors you cannot change include: Treatment Plan Selection. Inferring Transcriptional Module from Breast Cancer Profile Data
Breast cancer Inferring Transcriptional Module from Breast Cancer Profile Data Breast Cancer and Targeted Therapy Microarray Profile Data Inferring Transcriptional Module Methods CSC 177 Data Warehousing
More informationddm1a (PFG_3A-51065) ATG ddm1b (PFG_2B-60109) ATG osdrm2 (PFG_3A-04110) osdrm2 osdrm2 osdrm2
Relative expression.6.5.4.3.2.1 OsDDM1a OsDDM1b OsDRM2 TG TG TG ddm1a (PFG_3-5165) P1 P3 ddm1b (PFG_2-619) P2 531bp 5233bp P1 P4 P3 P2 F1 R1 F1 TG TG TG F1 R1 C ddm1a -/- -/- ddm1b -/- +/- +/- -/- D (PFG_3-411)
More informationGenetics Review. Alleles. The Punnett Square. Genotype and Phenotype. Codominance. Incomplete Dominance
Genetics Review Alleles These two different versions of gene A create a condition known as heterozygous. Only the dominant allele (A) will be expressed. When both chromosomes have identical copies of the
More informationNot IN Our Genes - A Different Kind of Inheritance.! Christopher Phiel, Ph.D. University of Colorado Denver Mini-STEM School February 4, 2014
Not IN Our Genes - A Different Kind of Inheritance! Christopher Phiel, Ph.D. University of Colorado Denver Mini-STEM School February 4, 2014 Epigenetics in Mainstream Media Epigenetics *Current definition:
More informationRegulation of Floral Organ Identity. Dr. Chloe Diamond Mara
Regulation of Floral Organ Identity Dr. Chloe Diamond Mara Flower Development Angiosperms (flowering plants) are the most widespread group of land plants Flowers are the reproductive organs that consist
More informationGenerating Mouse Models of Pancreatic Cancer
Generating Mouse Models of Pancreatic Cancer Aom Isbell http://www2.massgeneral.org/cancerresourceroom/types/gi/index.asp Spring/Summer 1, 2012 Alexandros Tzatsos, MD PhD Bardeesy Lab: Goals and Objectives
More informationOxi1 mutant plays an important role in Arabidopsis resistance against aphid (Myzus persicae)
Oxi1 mutant plays an important role in Arabidopsis resistance against aphid (Myzus persicae) Dr. Tahsin Shoala Assistant professor - College of Biotechnology - Misr University for Science and Technology
More informationSEX. Genetic Variation: The genetic substrate for natural selection. Sex: Sources of Genotypic Variation. Genetic Variation
Genetic Variation: The genetic substrate for natural selection Sex: Sources of Genotypic Variation Dr. Carol E. Lee, University of Wisconsin Genetic Variation If there is no genetic variation, neither
More informationGeneral Biology 1004 Chapter 11 Lecture Handout, Summer 2005 Dr. Frisby
Slide 1 CHAPTER 11 Gene Regulation PowerPoint Lecture Slides for Essential Biology, Second Edition & Essential Biology with Physiology Presentation prepared by Chris C. Romero Neil Campbell, Jane Reece,
More informationExpression of callose synthase genes and its connection with Npr1 signaling pathway during pathogen infection
Planta (2008) 229:87 98 DOI 10.1007/s00425-008-0812-3 ORIGINAL ARTICLE Expression of callose synthase genes and its connection with Npr1 signaling pathway during pathogen infection Xiaoyun Dong Zonglie
More informationSupplementary Figure 1 IL-27 IL
Tim-3 Supplementary Figure 1 Tc0 49.5 0.6 Tc1 63.5 0.84 Un 49.8 0.16 35.5 0.16 10 4 61.2 5.53 10 3 64.5 5.66 10 2 10 1 10 0 31 2.22 10 0 10 1 10 2 10 3 10 4 IL-10 28.2 1.69 IL-27 Supplementary Figure 1.
More informationFunctional validation of cancer susceptibility genes using gene editing
Functional validation of cancer susceptibility genes using gene editing 2-22-2017 Sabine Topka Research Fellow Niehaus Center for Inherited Cancer Genomics www.mskcc.org Inherited Predisposition to Cancer
More informationMuscular Dystrophy. Biol 405 Molecular Medicine
Muscular Dystrophy Biol 405 Molecular Medicine Duchenne muscular dystrophy Duchenne muscular dystrophy is a neuromuscular disease that occurs in ~ 1/3,500 male births. The disease causes developmental
More informationPsych 3102 Lecture 3. Mendelian Genetics
Psych 3102 Lecture 3 Mendelian Genetics Gregor Mendel 1822 1884, paper read 1865-66 Augustinian monk genotype alleles present at a locus can we identify this? phenotype expressed trait/characteristic can
More informationCan Brachypodium distachyon provide insight into FHB? Paul Nicholson Disease and Stress Biology Department John Innes Centre
Can Brachypodium distachyon provide insight into FHB? Paul Nicholson Disease and Stress Biology Department John Innes Centre Genetics and mechanisms of FHB resistance in wheat DON (mg kḡ 1 ) ET-silenced
More informationcontact info. Course coordinator can be reached at
Course Outline BGEN3020 Introduction to Human Genetics Course Coordinator: Dr. David Merz Department of Biochemistry and Medical Genetics Faculty of Health Sciences Rm 324 Basic Medical Sciences Building
More informationHLA and antigen presentation. Department of Immunology Charles University, 2nd Medical School University Hospital Motol
HLA and antigen presentation Department of Immunology Charles University, 2nd Medical School University Hospital Motol MHC in adaptive immunity Characteristics Specificity Innate For structures shared
More informationCourse Curriculum for Master Degree in Medical Laboratory Sciences / Diagnostic Molecular Biology and Human Genetics
Course Curriculum for Master Degree in Medical Laboratory Sciences / Diagnostic Molecular Biology and Human Genetics The Master Degree in Medical Laboratory Sciences / Diagnostic Molecular Biology & Human
More informationIntroduction. Introduction
Introduction We are leveraging genome sequencing data from The Cancer Genome Atlas (TCGA) to more accurately define mutated and stable genes and dysregulated metabolic pathways in solid tumors. These efforts
More informationMOLECULAR BIOLOGY AND GENETICS OF PROGRAMMED CELL DEATH IN ARABIDOPSIS THALIANA
PROCEEDINGS OF THE BALKAN SCIENTIFIC CONFERENCE OF BIOLOGY IN PLOVDIV (BULGARIA) FROM 19 TH TILL 21 ST OF MAY 2005 (EDS B. GRUEV, M. NIKOLOVA AND A. DONEV), 2005 (P. 157 161) MOLECULAR BIOLOGY AND GENETICS
More informationIntroduction to Genetics
Introduction to Genetics Table of contents Chromosome DNA Protein synthesis Mutation Genetic disorder Relationship between genes and cancer Genetic testing Technical concern 2 All living organisms consist
More informationThe functional investigation of the interaction between TATA-associated factor 3 (TAF3) and p53 protein
THESIS BOOK The functional investigation of the interaction between TATA-associated factor 3 (TAF3) and p53 protein Orsolya Buzás-Bereczki Supervisors: Dr. Éva Bálint Dr. Imre Miklós Boros University of
More informationThe lymphoma-associated NPM-ALK oncogene elicits a p16ink4a/prb-dependent tumor-suppressive pathway. Blood Jun 16;117(24):
DNA Sequencing Publications Standard Sequencing 1 Carro MS et al. DEK Expression is controlled by E2F and deregulated in diverse tumor types. Cell Cycle. 2006 Jun;5(11) 2 Lassandro L et al. The DNA sequence
More informationChapter 6- An Introduction to Viruses*
Chapter 6- An Introduction to Viruses* *Lecture notes are to be used as a study guide only and do not represent the comprehensive information you will need to know for the exams. 6.1 Overview of Viruses
More informationC. elegans Embryonic Development
Autonomous Specification in Tunicate development Autonomous & Conditional Specification in C. elegans Embryonic Development Figure 8.36 Bilateral Symmetry in the Egg of the Tunicate Styela partita Fig.
More informationSupplemental Figure S1. Tertiles of FKBP5 promoter methylation and internal regulatory region
Supplemental Figure S1. Tertiles of FKBP5 promoter methylation and internal regulatory region methylation in relation to PSS and fetal coupling. A, PSS values for participants whose placentas showed low,
More informationSignificance of the MHC
CHAPTER 7 Major Histocompatibility Complex (MHC) What is is MHC? HLA H-2 Minor histocompatibility antigens Peter Gorer & George Sneell (1940) Significance of the MHC role in immune response role in organ
More information7.012 Quiz 3 Answers
MIT Biology Department 7.012: Introductory Biology - Fall 2004 Instructors: Professor Eric Lander, Professor Robert A. Weinberg, Dr. Claudette Gardel Friday 11/12/04 7.012 Quiz 3 Answers A > 85 B 72-84
More informationFUNCTIONAL INVESTIGATION OF ARABIDOPSIS CALLOSE SYNTHASES AND THE SIGNAL TRANSDUCTION PATHWAY DISSERTATION. Xiaoyun Dong, M.S.
FUNCTIONAL INVESTIGATION OF ARABIDOPSIS CALLOSE SYNTHASES AND THE SIGNAL TRANSDUCTION PATHWAY DISSERTATION Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the
More informationThis document is a required reading assignment covering chapter 4 in your textbook.
This document is a required reading assignment covering chapter 4 in your textbook. Chromosomal basis of genes and linkage The majority of chapter 4 deals with the details of mitosis and meiosis. This
More informationGene Therapy. Definition: A disease resulting from a defect in individual genes. > 6000 inherited diseases 1:200 Births
Inherited Disease (AKA Genetic Disease): A disease that is caused by a defect in the genome and that, like other genetic features, can be passed from parents to offspring. Epigenetics: A heritable change
More informationChapter 7 Conclusions
VII-1 Chapter 7 Conclusions VII-2 The development of cell-based therapies ranging from well-established practices such as bone marrow transplant to next-generation strategies such as adoptive T-cell therapy
More informationAgro/Ansc/Bio/Gene/Hort 305 Fall, 2017 MEDICAL GENETICS AND CANCER Chpt 24, Genetics by Brooker (lecture outline) #17
Agro/Ansc/Bio/Gene/Hort 305 Fall, 2017 MEDICAL GENETICS AND CANCER Chpt 24, Genetics by Brooker (lecture outline) #17 INTRODUCTION - Our genes underlie every aspect of human health, both in function and
More informationChapter 4 Cellular Oncogenes ~ 4.6 -
Chapter 4 Cellular Oncogenes - 4.2 ~ 4.6 - Many retroviruses carrying oncogenes have been found in chickens and mice However, attempts undertaken during the 1970s to isolate viruses from most types of
More informationIntrinsic cellular defenses against virus infection
Intrinsic cellular defenses against virus infection Detection of virus infection Host cell response to virus infection Interferons: structure and synthesis Induction of antiviral activity Viral defenses
More informationThe Beauty of the Skin
The Beauty of the Skin Rose-Anne Romano, Ph.D Assistant Professor Department of Oral Biology School of Dental Medicine State University of New York at Buffalo The Big Question How do approximately 50 trillion
More informationAtrogin-1, a muscle-specific F-box protein highly expressed during muscle atrophy
(Courtesy of Magda Stumpfova. Used with permission.) Atrogin-1, a muscle-specific F-box protein highly expressed during muscle atrophy M.D. Gomes, S.H. Lecker, R.T. Jagoe, A. Navon, and A.L. Goldberg PNAS,
More informationVery-Long Chain Fatty Acid Biosynthesis
Very-Long Chain Fatty Acid Biosynthesis Objectives: 1. Review information on the isolation of mutants deficient in VLCFA biosynthesis 2. Generate hypotheses to explain the absence of mutants with lesions
More informationMendelian Genetics using Fast Plants Report due Sept. 15/16. Readings: Mendelian genetics: Hartwell Chapter 2 pp , Chapter 5 pp
1 Biology 423L Sept. 1/2 Mendelian Genetics using Fast Plants Report due Sept. 15/16. Readings: Mendelian genetics: Hartwell Chapter 2 pp. 13-27, Chapter 5 pp. 127-130. FASTPLANTS: Williams et al. (1986)
More informationNature Immunology doi: /ni Supplementary Figure 1. Raf-1 inhibition does not affect TLR4-induced type I IFN responses.
Supplementary Figure 1 Raf-1 inhibition does not affect TLR4-induced type I IFN responses. Real-time PCR analyses of IFNB, ISG15, TRIM5, TRIM22 and APOBEC3G mrna in modcs 6 h after stimulation with TLR4
More informationBIO360 Fall 2013 Quiz 1
BIO360 Fall 2013 Quiz 1 1. Examine the diagram below. There are two homologous copies of chromosome one and the allele of YFG carried on the light gray chromosome has undergone a loss-of-function mutation.
More informationUSABlight: A tool to fight late blight: a reemerging plant disease that threatens global food security
USABlight: A tool to fight late blight: a reemerging plant disease that threatens global food security JEAN BEAGLE RISTAINO WILLIAM NEAL REYNOLDS PROFESSOR DEPARTMENT OF ENTOMOLOGY AND PLANT PATHOLOGY
More informationDetermination of the temporal pattern and importance of BALF1 expression in Epstein-Barr viral infection
Determination of the temporal pattern and importance of BALF1 expression in Epstein-Barr viral infection Melissa Mihelidakis May 6, 2004 7.340 Research Proposal Introduction Apoptosis, or programmed cell
More information