Table S1. CDE Sequences and Globin Reporter mrna Half-Lives in NIH3T3 Cells, Related to Figures 1 and 2 Construct Sequence t1/2 ± SD (hrs)
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1 Table S1. CDE Sequences and Globin Reporter mrna Half-Lives in NIH3T3 Cells, Related to Figures 1 and 2 Construct Sequence t 1/2 ± SD (hrs) n TNF -CDE 37 -V2 AGATCCTTCAGACAGACATGTTTTCTGTGAAAACGGAGCTGAGCTAGATCT 8.8 ± TNF -CDE 37 -V3 AGATCCAGTTCAGACAGACATGTTTTCTGTGAAAACGGAGCTGAGCTAGATCT 1.4 ± human TNF -CDE 37 -V3 AGATCCTGTTGGCTCAGACATGTTTTCCGTGAAAACGGAGCTGAACAAGATCT 2.0 ± TNF -CDE 37 -V3-M1 AGATCCAGTTCAGA TTTTCTGTGAAAACGGAGCTGAGCTAGATCT > 9.0* 1 TNF -CDE 37 -V3-M2 AGATCCAGTTCAGACAGACATGTTT GCTGAGCTAGATCT > 9.0* 2 TNF -CDE 37 -V3-M3 AGATCCAGTAGTGACAGACATGTTTTCTGTGAAAACGGAGCTGAGCTAGATCT 1.5 ± TNF -CDE 37 -V3-M4 AGATCCAGTTCAGACAGACATGTTTTCTGTGAAAACGGAGCACTGCTAGATCT 2.5 ± TNF -CDE 37 -V3-M5 AGATCCAGTAGTGACAGACATGTTTTCTGTGAAAACGGAGCACTGCTAGATCT 1.1* 2 TNF -CDE 37 -V3-M6 AGATCCAGTTCAGAGTGACATGTTTTCTGTGAAAACGGAGCTGAGCTAGATCT 1.4 ± TNF -CDE 37 -V3-M7 AGATCCAGTTCAGACAGACAACTTTTCTGTGAAAACGGAGCTGAGCTAGATCT > TNF -CDE 37 -V3-M8 AGATCCAGTTCAGAGTGACAACTTTTCTGTGAAAACGGAGCTGAGCTAGATCT > 9.0* 2 TNF -CDE 37 -V3-M9 AGATCCAGTTCAGACAGACATGTTTAGGCTGAAAACGGAGCTGAGCTAGATCT > TNF -CDE 37 -V3-M10 AGATCCAGTTCAGACAGACATGTTTTCTGTGAAAAGTCTGCTGAGCTAGATCT > 9.0* 1 TNF -CDE 37 -V3-M11 AGATCCAGTTCAGACAGACATGTTTAGGCTGAAAAGTCTGCTGAGCTAGATCT > 9.0* 1 TNF -CDE 37 -V3-M12 AGATCCAGTTCAGACAGACATGTTTTCTGTGAAAACGCTGCTGAGCTAGATCT 1.3 ± TNF -CDE 37 -V3-M13 AGATCCAGTTCAGACAGACATGTTTAGTGTGAAAACGCTGCTGAGCTAGATCT > TNF -CDE 37 -V3-M14 AGATCCAGTTCAGACAGACATGTTTTCTGTGAAAAGTGAGCTGAGCTAGATCT > TNF -CDE 37 -V3-M15 AGATCCAGTTCAGACAGACATGTTTTCGCTGAAAAGTGAGCTGAGCTAGATCT > TNF -CDE 37 -V3-M16 AGATCCAGTTCAGACAGACATGTTTTCTGTGAAATGGGAGCTGAGCTAGATCT > TNF -CDE 37 -V3-M17 AGATCCAGTTCAGACAGACATGTTTTCTCAGAAATGGGAGCTGAGCTAGATCT > TNF -CDE 37 -V3-M18 AGATCCAGTTCAGACAGAGTTGTTTTCTGTGAAAACGGAGCTGAGCTAGATCT 2.0 ± TNF -CDE 37 -V3-M19 AGATCCAGTTCAGACAGACATCATTTCTGTGAAAACGGAGCTGAGCTAGATCT > TNF -CDE 37 -V3-M20 AGATCCAGTTCAGACAGACATCATTTCTGTGAAATGGGAGCTGAGCTAGATCT 2.7 ± TNF -CDE 37 -V3-M21 AGATCCAGTTCAGACAGACATGTTTAGTGTGAAAACGGAGCTGAGCTAGATCT > TNF -CDE 37 -V3-M22 AGATCCAGTTCAGACAGACATGTTTTCTGTCTAAACGGAGCTGAGCTAGATCT > TNF -CDE 37 -V3-M23 AGATCCAGTTCAGACAGACATGTTTAGTGTCTAAACGGAGCTGAGCTAGATCT > TNF -CDE 37 -V3-M24 AGATCCAGTTCAGACAGACATGTTTTCTATGAAAACGGAGCTGAGCTAGATCT 2.2 ± TNF -CDE 37 -V3-M25 AGATCCAGTTCAGACAGACATGTTTTCACAGAAAACGGAGCTGAGCTAGATCT > TNF -CDE 37 -V3-M26 AGATCCAGTTCAGACAGACATGTATTCTGTGAATACGGAGCTGAGCTAGATCT 5.0 ± TNF -CDE 37 -V3-M27 AGATCCAGTTCAGACAGACATGTTATCTGTGATAACGGAGCTGAGCTAGATCT > TNF -CDE 17 -ds AGATCCAGTGTTTTCTGTGAAAACGCTAGATCT > TNF -CDE 17 -ss TCTAGATGTTTTCTGTGAAAACGACCGGT 1.1 ± ICOS-CDE 17 -ss TCTAGAAAATTTCTGTGAAATAAACCGGT 4.8 ± TNF -CDE 150 AGATCCTTTGGAAGGCCGGGGTGTCCTGGAGGACCCAGTGTGGGAAGCTGTCTTCAGACAG ACATGTTTTCTGTGAAAACGGAGCTGAGCTGTCCCCACCTGGCCTCTCTACCTTGTTGCCT CCTCTTTTGCTTATGTTTAAAACAAAATATTTATAGATCT 5.6 ± TNF -CDE 150 -M16 AGATCCTTTGGAAGGCCGGGGTGTCCTGGAGGACCCAGTGTGGGAAGCTGTCTTCAGACAG ACATGTTTTCTGTGAAATGGGAGCTGAGCTGTCCCCACCTGGCCTCTCTACCTTGTTGCCT > CCTCTTTTGCTTATGTTTAAAACAAAATATTTATAGATCT TNF -CDE 150 -M20 AGATCCTTTGGAAGGCCGGGGTGTCCTGGAGGACCCAGTGTGGGAAGCTGTCTTCAGACAG ACATCATTTCTGTGAAATGGGAGCTGAGCTGTCCCCACCTGGCCTCTCTACCTTGTTGCCT 5.6 ± CCTCTTTTGCTTATGTTTAAAACAAAATATTTATAGATCT XXX CDE sequence XXX nucleotides introduced into cloning context XXX mutation --- deletion XXX cloning sites XXX flanking TNF 3 UTR sequence in CDE 150 XXX primer sites * mrna half-lives determined upon stable transfection
2 Table S2. Proteins Enriched by CDE RNA Affinity Purification, Related to Figure 3 Name Symbol MW (kda) No of Peptides 4xS1m CDE-4xS1m Roquin, RING finger and CCCH zinc finger protein 1 Rc3h Cleavage and polyadenylation specificity factor subunit 7 Cpsf Ribosome biogenesis regulatory protein homolog Rrs Roquin2, RING finger and CCCH zinc finger protein 2 Rc3h Nucleophosmin Npm Splicing factor, proline- and glutamine-rich Sfpq Fragile X mental retardation syndrome-related protein 1 Fxr
3 Table S3. Proteins Purified with Roquin and Roquin2, Related to Figure 5 No of peptides Protein MW (kda) EGFP EGFP-Roquin EGFP-Roquin2 CNOT1 = Not RC3H1 = Roquin PABP TAB182 = TNKS1BP IF2B RC3H2 = Roquin PHB GBLP YBOX HNRPM IF2B MOV RCD1 = Not PABP STML PDIA DHX DBPA CNOTA = Not HNRPC P5CR CNOT2 = Not DDX VIME CB PAIRB AGAL IF2B LARP KCRB KPYM CNOT3 = Not RENT AFG ZCHC AL7A PCBP ILF PDIA DHB RBMX DDX3X AAAT CNO6L = Ccr4b CNOT8 = Caf1b
4 CNOT7 = Caf1a Blue: bait proteins Yellow: proteins of the Ccr4-Caf1-Not complex
5 Table S4. Bioinformatics Prediction of CDEs in Mouse 3 UTRs, Related to Figure 6 Name GeneID Genomic Coordinates CDE Sequence* Stem (nt) Conservation Bmpr1a chr14(-): GCTTTCTGTGAAAGC 6 41 Fos chr12(+): GTTTTCCATGAAAAC 6 40 D11Wsu99e chr11(-): CCTTCTGTGAAGG 5 38 Pdia chr12(+): AATTCTATGAATT 5 38 Prkg chr19(-): CATTTCTGTGAAATG 6 38 Fbxo chr17(-): TCTTTCTATGAAAGA 6 38 Slc25a chr2(-): CATTCTGTGAATG 5 37 Brwd chr16(-): TTTTCTATGAAAA 5 36 Nfkbiz chr16(-): TTGTTTCTATGAAACAA 7 36 Epc chr18(-): TCTCTTCTGTGAAGAGA 7 36 Adamts chr13(+): ACTTCTGTGAAGT 5 36 Baz2b chr2(-): TTTTCTGTGAAAA 5 36 Kpna chr3(-): TTTTCTATGAAAA 5 35 Dscaml chr9(+): TTTTTCTATGAAAAA 6 35 Bptf chr11(-): CTTTCTATGAAAG 5 35 Rc3h chr2(-): AACTTCTGTGAAGTT 6 34 Lpin chr17(+): TCTTTCTGTGAAAGA 6 34 Hmgxb chr18(-): CCCTTCTGTGAAGGG 6 34 Slc35f chr14(-): TATATTCTGTGAATATA A02Rik chr16(+): CCTTCTGTGAAGG 5 34 Frmd chr12(+): TCCTTTCTGTGAAAGGA 7 34 Rc3h chr1(+): AACTTCTGTGAAGTT 6 34 Phf chr4(-): GTTTCTGTGAAAC 5 33 Edem chr1(+): CATTCTGTGAATG 5 33 Tnf chr17(-): GTTTTCTGTGAAAAC 6 33 Aggf chr13(-): TTCTTCTGTGAAGAA 6 33 Uhrf1bp chr17(+): TGGTTCTGTGAACCA 6 33 Ctla chr1(+): ATTTTCCATGAAAAT 6 32 Lpin chr12(-): TCTTTCTGTGAAAGA 6 32 Prkd chr12(-): TGTTCTATGAACA 5 32 Gramd chr18(+): CATTTCTATGAAATG 6 32 Crispld chr8(+): TTTTTCTATGAAAAA 6 31 Plag chr4(-): GTTTCTATGAAAC 5 31 Mdga chr12(-): TTTTCTGTGAAAA 5 30 Wnt3a chr11(-): TTTCTTTCTATGAAAGAAA 8 30 Rbbp chr1(+): CCTTTCTGTGAAAGG 6 28 Slc25a chrx(+): TCTTCTGTGAAGA 5 28 Scmh chr4(+): GTCCTTCCATGAAGGAC 7 27 Efna chr3(-): GTCTTCTGTGAAGAC 6 27
6 Icos chr1(+): ATTTCTGTGAAAT 5 26 Atp2b chr10(+): ATTTCTGTGAAAT 5 26 Ppp1r chr17(+): TGTTTTCTATGAAAACA 7 26 Nfkbid chr7(+): GATTTCTGTGAAATC 6 25 Bcan chr3(-): TACTTCTGTGAAGTA 6 25 Nfkbid chr7(+): TGTTTCTGTGAAACA 6 23 Sertad chr7(+): CCTTCCGTGAAGG 5 23 Pdzk1ip chr4(+): TTATTTCTGTGAAATAA 7 21 Ier chr17(+): TGTTCTGTGAACA 5 21 Agap chr10(+): CCCTTTCCGTGAAAGGG 7 20 Kctd chr5(-): ATTTCTGTGAAAT 5 20 Shprh chr10(+): TCTTCTGTGAAGA 5 20 Pex chr11(-): GTTTCTATGAAAC 5 19 Prr chr17(-): TGTTTTCTGTGAAAACA 7 19 Zfp chr7(-): AACATTCTGTGAATGTT 7 15 Fam188b chr6(+): CCTTCCATGAAGG 5 13 Chic chrx(+): TTGTTTCCATGAAACAA 7 11 Dcaf chr4(-): ACTTTCTGTGAAAGT 6 9 Rps15a chr7(-): CATTTCTGTGAAATG 6 8 Fam63b chr9(-): ATTTCTGTGAAAT 5 5 Cdc14a chr3(-): TGTTCTATGAACA 5 4 Hes chr4(+): ACTTCTGCGAAGT K19Rik chr16(-): AGTTCTGTGAACT 5 3 Hdx chrx(-): TATTCTATGAATA 5 3 Arhgef chr3(+): CTTTCTACGAAAG 5 2 Tnks chr19(+): CTTTCCATGAAAG 5 2 Tcf chr9(-): ATTTCCATGAAAT 5 2 Tmem chr9(+): TATTCCATGAATA 5 2 Dagla chr19(-): TATTCTATGAATA 5 2 Ifi chr1(-): TTTTCTATGAAAA 5 2 Pxdn chr12(+): ACTTCTGTGAAGT 5 2 C330018D20Rik chr18(-): AGTTCTGTGAACT 5 2 Gpr chr5(+): CCTTCCGTGAAGG 5 2 Steap chr1(-): CCTTCTGCGAAGG 5 2 Pak chr7(+): CTGTTTCTACGAAACAG 7 2 Sftpb chr6(+): ATTTCTATGAAAT 5 2 Yipf chr17(+): TATTCCGCGAATA 5 2 Cnot chr8(-): TGTTCCGTGAACA 5 1 Pkib chr10(+): TTTTCTATGAAAA 5 1 Homez chr14(-): ATTTCTATGAAAT 5 1 Gng chr6(+): AAATTTCCGTGAAATTT 7 1
7 Gtf3c chr2(-): ATTTCTGTGAAAT 5 1 Tdg chr10(+): TGTTCTGTGAACA 5 1 Fyco chr9(-): CTTTCTATGAAAG 5 1 Kcnj chr16(-): CTTTCTGTGAAAG 5 1 Rpusd chr9(+): ATTTCTGTGAAAT 5 1 Msx chr13(-): ATTTCTATGAAAT 5 1 D630042F21Rik chr6(+): TGTTCTGTGAACA 5 1 Lrrcc chr3(+): CATTCCATGAATG B07Rik chr13(+): AGTTCTATGAACT 5 1 Mnda chr1(-): TTTTCTATGAAAA 5 1 Ccdc chr16(+): CCTCCTTCTGCGAAGGAGG 8 1 Sh3bp chr5(+): TTTTCTGTGAAAA 5 1 # Pdcd chr13(-): CAATTCTGTGAATTG 6 1 # Slc27a chr2(+): CTTTCTATGAAAG 5 1 # Zfp chr7(-): CTTTCTATGAAAG 5 1 # BC chr16(+): TGTTTCTATGAAACA 6 1 # Mndal chr1(-): TTTTCTATGAAAA 5 1 # Gvin chr7(-): ATTTTCTATGAAAAT 6 1 # Gm chr7(-): ATTTTCTATGAAAAT 6 1 # Mau chr8(-): CCTTCTGTGAAGG 5 1 # Dap chr3(-): TTTTCTGTGAAAA 5 1 # Slc7a chr3(+): TTATTCTATGAATAA 6 1 # Crispld chr1(+): TTTTCCATGAAAA 5 1 # Lrrtm chr6(+): ACTTTCTATGAAAGT 6 1 # A18Rik chr10(-): GTTTCTGTGAAAC 5 1 # Lilrb chr10(+): CATTCCATGAATG 5 1 # Gp49a chr10(+): CATTCCATGAATG 5 1 # Zfp chr8(-): ATTTCTGTGAAAT 5 1 # Afg3l chr8(+): CGTTCCGTGAACG 5 1 # * Stem depicted in black, loop depicted in red Number of species with a conserved CDE at this genomic position in the UCSC 46 vertebrate species alignment # Alignment at this genomic position was not available Yellow: CDEs conserved in > 10/46 vertebrate species
8 Table S5. Functional Categories of Genes Containing Conserved 3 UTR CDEs, Related to Figure 6 Gene ontology term (biological process) observed Number of genes expected Regulation of developmental process Transcription, DNA-dependent Regulation of RNA metabolic process RNA biosynthetic process Nucleic acid metabolic process Negative regulation of T-cell differentiation
9 Table S6. mrnas Significantly Enriched by Roquin-IP, Related to Figure 6 Name GeneID CDE p-value (Roquin-IP vs control-ip) fold enrichment (Roquin-IP / control-ip) fold enrichment (Roquin-IP / input) Nfkbid UTR, conserved (2) 2.1E Tnf (TNFα) UTR, conserved 1.1E Irf E Gm E D5Ertd579e ORF (2) 3.7E Rc3h1 (Roquin) UTR, conserved 1.8E Tcf7l E Csf3 (G-CSF) E Id E Slc39a E Zfp E Calcrl E Rc3h2 (Roquin2) UTR, conserved 6.0E Ptger E Ptpre E Id E Mgat E Adamts E Clk E Nfkbiz UTR, conserved 4.9E Ap1g E Usp E B630005N14Rik E Map3k E Id E Pex E Smek E C07Rik E Bmpr1a UTR, conserved 1.5E Icos UTR, conserved 1.7E Zbtb E Ier UTR, conserved 8.1E Fbxo E Pik3ca E Rybp E Tigd E Ss18l E Lfng E Pptc E Rsf E Lysmd E Mlec E Fam160b E
10 D030056L22Rik E Sec24c E Tmem E F09Rik E Cenpf E Etaa E Nedd E Ipo ORF (2) 9.3E Ppan E Ppp1r UTR, conserved 2.1E Tm2d E Ndst E Slc35e E Prr UTR, conserved 5.6E Agbl E Msl E Hinfp E Kctd E Nuf E Cgrrf E Rbm E Aggf UTR, conserved 3.7E Pole ORF 2.2E Papolg E Tfap E Fam76b E Cdc E D21Rik E Dnajc E Cdon E Ap1s E Nfxl E Sacm1l E Zfp E Hcst E Dcun1d E Fbxo UTR, conserved 8.8E B4galt ORF 7.4E Mthfd E Mrps E Rbbp UTR, conserved 2.9E Ube2q E E20Rik E D19Rik E Rabl E Hmgxb UTR, conserved 1.6E Lig E Fam84b E Epc UTR, conserved 7.0E
11 Dpy19l E AI E Pik3cb E Purple: mrna significantly enriched by Roquin-IP that contains a conserved CDE in the 3 UTR Green: mrna significantly enriched by Roquin-IP that contains a CDE in the ORF
12 Table S7. DNA Oligonucleotides, Related to Experimental Procedures Name Sequence Description G078 GGTGGTCGGAAAGCTATC S7 probe F G083 TTACAAAGTCACTCAGGATG Ncl probe R G314 TTAAGCTAGCAGGGAATGGG mouse TNF probe F G316 GCCGATTTAGGTGACACTATAGAATACTGGCTCTGTGAGG mouse TNF probe R G1000 GTGCATCTGTCCAGTG globin probe R G1001 GCCGATTTAGGTGACACTATAGAATACCCTGAAGTTCTC globin probe F G1008 GCCGATTTAGGTGACACTATAGAATACTATAGACACCAG S7 probe R G1009 GCCGATTTAGGTGACACTATAGAATACTTAGCGTCTTCG Ncl probe F G1706 AGGGGGCAGAAATTGATGGACGAT mouse Ncl qpcr F G1707 TGGGTTCTGGGGCACTTTG mouse Ncl qpcr R G1712 GAGCGGGCCGTGGTGTC mouse Ier3 qpcr F G1713 CTTGGCAATGTTGGGTTCCTC mouse Ier3 qpcr R G1844 TGCCTATGTCTCAGCCTCTTC mouse TNFα qpcr F G1845 GAGGCCATTTGGGAACTTCT mouse TNFα qpcr R G2053 ACTCCACTCACGGCAAATTCAACG mouse Gapdh qpcr F G2054 AAGACACCAGTAGACTCCACGACA mouse Gapdh qpcr R G2181 CAGGTGAACACCACGGATT mouse Nfkbiz qpcr F G2182 CTCACAGCTCCCTTCTGGAT mouse Nfkbiz qpcr R G2199 ATATTCGTGAACATAAAGGCAAGA mouse Nfkbid qpcr F G2200 TCAGTGGCGTTAGGCTCTG mouse Nfkbid qpcr R G2234 CCAAGTAATACGACTCACTATAGGCCTGGCTCACAAATACCACTG pt7-in-line F G2235 AATTTTTGGCAGAGGGAAAAAG in-line R G2332 CCAAGTAATACGACTCACTATAGGTTTGGAAGGCCGGGGT pt7-cde 150 mouse in-line F G2333 ATAAATATTTTGTTTTAAACATAAGCAAAAGAG CDE 150 mouse in-line R G2364 CATGGCTGCAACACTTACACAGCA mouse Nupl1 qpcr F G2365 ATTGCAAGCCAGTGCCAATACCTG mouse Nupl1 qpcr R G2392 TTGTACCTGAAGCCACTCAGCAGT mouse Roquin qpcr F G2393 TCCACTAGCTGGCAATGAACCAGA mouse Roquin qpcr R G2394 TGCCCATTCTCAGGAAGAGCTTGA mouse Roquin2 qpcr F G2395 GCTGTGGTTGTGACAGTGCTGTTT mouse Roquin2 qpcr R G1612 CCCAAGCTTGATATCATCCACCATGGTGAGCAAG EGFP F, HindIII-EcoRV G1613 TTAAGGTACCCTTGTACAGCTCGTCCATGCCGAG EGFP R, KpnI G2421 TGGACACAGTATCATACCCAGAAAACACCTGTCTCTTCAACTCTTCCTGTGGCCA mouse Roquin2 cdna F G2427 GATCATCGATTCAGCTGTTAACCATCTTCCCA mouse Roquin2-ClaI cdna R G2495 GATCGGATCCTTTGGAAGGCCGGGGT CDE 150 -BamHI-outF G2496 GATCAGATCTATAAATATTTTGTTTTAAACATAAGCAAAAGAG CDE 150 -outr-bglii G2497 TTCTGTGAAATGGGAGCTGAG CDE 150 -M16 F G2498 TTTCACAGAAAACATGTCTGTCTGA CDE 150 -M16 R G2501 GACAGACATCATTTCTGTGAAATGGGA CDE 150 -M20 F G2502 ATGTCTGTCTGAAGACAGCTTCC CDE 150 -M20 R G2554 AAACTAGTATGCGCCGAGATTTACGA Roquin2 cdna C413R F G2555 CATACTAGTTTTATACTTGCTGTTTGGCTG Roquin2 cdna C413R R G2556 GATCCAATTGTTTTGCAGCGAACTG Roquin2-MfeI cdna F G2576 AACATACATGCGTCGAGATATGAAG Roquin cdna C419R F
13 G2577 CATGTATGTTTTGTATTTGCTATGCTGT Roquin cdna C419R R G2579 GATCCACATGGTGGTGGAGTATAGTACATACC Roquin-DraIII cdna R G2592 TTCCCCCTGTTGAAGTTCC mouse Ppp1r10 qpcr F G2593 CTTGCGAGTCAGCTGGTTG mouse Ppp1r10 qpcr R G2622 GATACTGTGTCCACTGATCTGGC BstXI-Roquin2 cdna R G2623 TCATTTGCACAGAGTGTTCAGG Roquin-DraIII cdna F G2723 TGACCTGGGCCTAGCTGTTA mouse Bmpr1a qpcr F G2724 TTCAGGCTTTCATCCAGCA mouse Bmpr1a qpcr R G2729 CTCACATCCTGGACACTGGA mouse Pdia6 qpcr F G2730 ACTGGGCTCCAGCTTCTGT mouse Pdia6 qpcr R G2737 TGTTGGCCCTCTATGAATCTG mouse Hmgxb3 qpcr F G2738 TGGAACCACAGACCACCTG mouse Hmgxb3 qpcr R G2747 TTCATCGTATTGGAAGATCAGG mouse Rck qpcr F G2748 TTGGGATGGGTTTGATCTCT mouse Rck qpcr R G2788 AGGGACCGGTGTCGACC AgeI-SalI-Roquin flanking fr1 F G2789 AGAGCAATTGTTAGCTCCTGAACA MfeI-Roquin flanking fr2 R G2790 GTGACCGAACTGATTCTCCAGCACCAG sirna-resistant Roquin fr2 F G2791 CAGTTCGGTCACTGTGCGTTCACCTAAAG sirna-resistant Roquin fr1 R G2840 ATTAATCGATCTATTCCTGTGAATGTG ClaI-Stop-N-term R G2842 TTATACCGGTGGAACTGGAAAAG Roquin C-term F G2843 GTCCCACGTGGAATCAGTTG Roquin C-term R G2855 TTATACCGGTGCGCCCAATGCAGAGGAA ROQ-AgeI F G2856 TATAATCGATGAGTAACTCCAAATGGGGTCTTAG ROQ-ClaI R
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