Quality Diagnostic Tests for Poultry Industry

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1 Quality Diagnostic Tests for Poultry Industry VIRAL DISEASES A. DETECTING ANTIBODIES/host immune response against virus infection/vaccination Sample type: Serum Minimum sample: 15 samples per test 11ELSA-E031 Newcastle Disease Virus (SYN) NDV Indirect ELISA/Synbiotics 11ELSA-E003 Newcastle Disease Virus (IDX) NDV Indirect ELISA/IDEXX 11HEIN-E042 Newcastle Disease Virus (Haemagglutination Inhibition) ND/HI HI Test 11ELSA-E048 Newcastle Disease Virus (Biochek) NDV Indirect ELISA/Biochek 11ELSA-E040 Infectious Bronchitis Virus (SYN) IBV Indirect ELISA/Synbiotics 11ELSA-E001 Infectious Bronchitis Virus (IDX) IBV Indirect ELISA/IDEXX 11ELSA-E049 Infectious Bronchitis Virus (Biochek) IBV Indirect ELISA/Biochek 11ELSA-E006 Swollen Head Syndrome/Avian Metapneumovirus (IDX) SHS/APV Indirect ELISA/IDEXX 11ELSA-E029 Infectious Bursal Disease Virus (SYN) IBDV Indirect ELISA/Synbiotics 11ELSA-E030* Infectious Bursal Disease Virus IBD Plus+ (SYN) IBDV Indirect ELISA/Synbiotics 11ELSA-E002 Infectious Bursal Disease Virus (IDX) IBDV Indirect ELISA/IDEXX 11ELSA-E050 Infectious Bursal Disease Virus (Biochek) IBDV Indirect ELISA/Biochek 11ELSA-E035 Reovirus/Avian Arthritis Virus (SYN) REO Indirect ELISA/Synbiotics 11ELSA-E004 Reovirus/Avian Arthritis Virus (IDX) REO Indirect ELISA/IDEXX 11ELSA-E038 Chicken Anaemia Virus (SYN) CAV Indirect ELISA/Synbiotics 2012 Vet Food Agro Diagnostics (M) Sdn Bhd ( X) Page 1 of 11

2 11ELSA-E028 Chicken Anaemia Virus (IDX) CAV Blocking ELISA/IDEXX 11ELSA-E024* Egg Drop Syndrome Virus (HIP) EDS Indirect ELISA/HIPRA 11HEIN-E041 Egg Drop Syndrome Virus (Haemagglutination EDS HI Test Inhibition) 11ELSA-E039 Avian Influenza Virus Flu Detect BE AIV (SYN) AI Blocking ELISA/Synbiotics 11ELSA-E014 Avian Influenza Virus (IDX) AI Indirect ELISA/IDEXX 11ELSA-E054* Avian Leukosis Virus Subgroup J (SYN) ALV-J Indirect ELISA/Synbiotics 11ELSA-E055* Avian Influenza Virus (SYN) AIV Indirect ELISA/Synbiotics B. DETECTING VIRAL ANTIGENS (FIELD TEST) with immunoassays (Lateral Flow) Sample type: Tracheal swab Minimum sample: 15 samples per test 11LAFL-E043 Avian Influenza Virus Type A Flu Detect (SYN) AI Dipstick (Rapid field test) /Synbiotics C. DETECTING VIRAL ANTIGENS (PCR) with molecular assays Sample type: Animal tissues, organ, swab, food, feed, environmental samples 31RPCR-M053* Newcastle Disease Virus NDV Real-time PCR/APSN 31CPCR-M004 Newcastle Disease Virus NDV Conventional PCR/APSN 31RPCR-M055* Infectious Bronchitis Virus Screening IBV Real-time PCR/APSN 31CPCR-M005 Infectious Bronchitis Virus (793B/D274/MASS) IBV Conventional PCR/APSN 31RPCR-M056* Avian metapneumovirus SHS/APV Real-time PCR/APSN 31CPCR-M002 Swollen Head Syndrome/Avian metapneumovirus SHS/APV Conventional PCR/APSN 31RPCR-M054* Infectious Bursal Disease Virus IBDV Real-time PCR/APSN 31CPCR-M006 Infectious Bursal Disease Virus IBDV Conventional PCR/APSN 31RPCR-M052* Reovirus REO Real-time PCR/APSN 31CPCR-M007 Reovirus REO Conventional PCR/APSN 2012 Vet Food Agro Diagnostics (M) Sdn Bhd ( X) Page 2 of 11

3 31CPCR-M003 Chicken Anaemia Virus CAV Conventional PCR/APSN 31RPCR-M031* Adenovirus Group 1 EDS Real-time PCR/APSN 31CPCR-M017 Infectious Laryngotracheitis (ILT)/Herpesvirus ILT Conventional PCR/APSN 31CPCR-M067* Egg Drop Syndrome/Adenovirus Adeno Conventional PCR/APSN 31RPCR-M032* Marek s Disease Virus Serotype 1 (Gallid herpesvirus) Marek s Real-time PCR/APSN 31RPCR-M033* Marek s Disease Virus Serotype 2 (Gallid herpesvirus) Marek s Real-time PCR/APSN 31RPCR-M034* Marek s Disease Virus Serotype 3 (Gallid herpesvirus) Marek s Real-time PCR/APSN 31RPCR-M039* Avian Influenza Virus H5N1 H5N1 Real-time PCR/APSN 31RPCR-M074 DIVA assay-reovirus REO Real-time PCR/APSN 31RPCR-M075 DIVA assay-newcastle Disease Virus NDV Real-time PCR/APSN 31RPCR-M076 DIVA assay-infectious Bursal Disease Virus IBDV Real-time PCR/APSN 31RPCR-M077 DIVA assay-avian metapneumovirus APV Real-time PCR/APSN 31RPCR-M083 Infectious Bronchitis Virus (793/B) IBV Real-time PCR/APSN 31RPCR-M084 Infectious Bronchitis Virus (Massachusetts) IBV Real-time PCR/APSN 31RPCR-M085 Mycoplasma gallisepticum (MG) MG Real-time PCR/APSN 31RPCR-M086 Mycoplasma synoviae (MS) MS Real-time PCR/APSN 31CPCR-M104* Avian Encephalomyelitis (AE) AEV Conventional PCR/APSN 31CPCR-M105* Avian Leukosis Virus Subtype J ALVJ Conventional PCR/APSN BACTERIAL DISEASES A. DETECTING ANTIBODIES/host immune response against bacterial infection/vaccination Sample type: Serum Minimum sample: 15 samples per test 11ELSA-E036* Mycoplasma gallisepticum (SYN) MG Indirect ELISA/Synbiotics 2012 Vet Food Agro Diagnostics (M) Sdn Bhd ( X) Page 3 of 11

4 11ELSA-E005 Mycoplasma gallisepticum (IDX) MG Indirect ELISA/IDEXX 11RPAT-E045* Mycoplasma gallisepticum MS Plate Agglutination Test (Field Test)/Charles River 11ELSA-E037* Mycoplasma synoviae (SYN) MS Indirect ELISA/Synbiotics 11ELSA-E032* Mycoplasma synoviae (IDX) MS Indirect ELISA/IDEXX 11RPAT-E044* Mycoplasma synoviae MG Plate Agglutination Test (Field Test)/Charles River 11ELSA-E027 Ornithobacterium rhinotracheale ORT Indirect ELISA/IDEXX (IDX) 11ELSA-E047 Salmonella Group B and D (Biochek) SE/ST Biochek ELISA 11ELSA-E059 Pasteurella multocida (SYN) PM Indirect ELISA/Synbiotics B. BACTERIAL CULTURE AND IDENTIFICATION Sample type: Water, feed, food, meat, swab, organ, environmental samples TEST CODE 21BACI-B038 21BACI-B002* 21BACI-B001 21BACI-B003 21BACI-B004* 21BACI-B005* 21BACI-B006 21BACI-B007* 21BACI-B008* 21BACI-B010* 21BACI-B011* 21BACI-B042* 21BACI-B043* DISEASE/PATHOGEN Bacterial Identification (Up To Species Level) General Bacterial Identification (Up to Genus Level) Salmonella Isolation and Serotyping Vancomycin Resistant Enterococci (VRE) Screening Vancomycin Resistant Enterococci (VRE) Species Typing Staphylococcus aureus Methicillin-Resistant Staphylococcus aureus (MRSA) Vancomycin-Resistant Staphylococcus aureus Shigella spp. Escherichia coli Clostridium perfringens and Clostridium spp. Campylobacter spp. Listeria spp Vet Food Agro Diagnostics (M) Sdn Bhd ( X) Page 4 of 11

5 E. BACTERIAL CULTURE AND ENUMERATION/COUNT Sample type: Water, feed, food, meat, swab, organ, liquids, solids, environmental samples TEST CODE 21BAMC-B013 21BAMC-B014* 21BAMC-B015 21BAMC-B016 21BAMC-B017 21BAMC-B018 21BAMC-B019 21BAMC-B021* 21BAMC-B022 21BAMC-B023* BACTERIAL COUNT Enterobacteriaceae Count (Culture and Enumeration) Clostridium perfringens Count (Culture and Enumeration) Standard Plate Count (Culture and Enumeration) Total Coliform Count (Culture and Enumeration) E. coli Count (Culture and Enumeration) Total Yeast and Mould Count (Culture and Enumeration) Yeast Count (Culture and Enumeration) Pseudomonas Count (Culture and Enumeration) Coagulase-Positive Staphylococci (Culture and Enumeration) Lactic Acid Bacteria Count (Culture and Enumeration) F. BACTERIAL COUNT ON EXPOSED AGAR PLATES Sample type: Exposed agar plates TEST CODE 21BAMC-B025* 21BAMC-B026* 21BAMC-B027* 21BAMC-B028* BACTERIAL COUNT Standard Plate Count (Plate Counting) Total Coliform /E. coli Count (Plate Counting) Total Yeast and Mould Count (Plate Counting) Pseudomonas Count (Plate Counting) G. ANTIMICROBIAL INHIBITION TESTS Sample type: Bacteria TEST CODE ANTIMICROBIAL SENSITIVITY TESTS DESCRIPTION/BRAND 21BAMC-B030 Antimicrobial Sensitivity/Susceptibility Test - 5 Discs (Excluding bacterial isolation) Choice of antibiotic discs: 30 types of antibiotics Antibiotic Sensitivity (5 Discs) Pick 5 antibiotics discs to test the susceptibility of the bacterium (one species) on agar plate 2012 Vet Food Agro Diagnostics (M) Sdn Bhd ( X) Page 5 of 11

6 TEST CODE ANTIMICROBIAL SENSITIVITY TESTS DESCRIPTION/BRAND available; please ask for the availability of preferred antibiotics 21BAMC-B031 Antimicrobial Sensitivity/Susceptibility Test - 10 Discs (Excluding bacterial isolation) Choice of antibiotic discs: as above Antibiotic Sensitivity (10 Discs) Pick 10 antibiotics discs to test the susceptibility of the bacterium (one species) on agar plate 21BAMC-B032* Minimum Inhibition Concentration - Broth Dilution MIC Broth Pick 5 antibiotics to determine the (Excluding bacterial isolation) Choice of antibiotic: as above Dilution exact minimum concentrations (ug/ml) that can inhibit the bacterium (one species) 21BAMC-B033* Minimum Inhibition Concentration - Agar Dilution MIC Agar Pick 5 antibiotics to determine the (Excluding bacterial isolation) Choice of antibiotic: as above Dilution exact minimum concentrations (ug/ml) that can inhibit the bacteria (up to 2 species) H. RAPID DETECTION OF BACTERIA (PCR) with molecular assays Sample type: Animal tissues, organ, swab, food, feed, environmental samples 31CPCR-M001 Mycoplasma gallisepticum/mycoplasma synoviae MG/MS Conventional PCR/APSN 31CPCR-M012 Salmonella spp. Salmonella Conventional PCR/APSN 31CPCR-M008 Multiplex Vancomycin Resistant Enterococci (VRE- VRE Conventional PCR/APSN vana/vanb/vanc) 31RPCR-M019* Vancomycin Resistant Enterococci - vana vana Real-time PCR/APSN 31RPCR-M020* Vancomycin Resistant Enterococci - vanb vanb Real-time PCR/APSN 31RPCR-M021* Vancomycin Resistant Enterococci - vanc vanc Real-time PCR/APSN 31RPCR-M035 Multiplex MRSA (meca) & VRE (vana) MRSA & VRE Real-time PCR/APSN 31CPCR-M013 Staphylococcus aureus Staph Conventional PCR/APSN 31CPCR-M009* Campylobacter spp. Campylo Conventional PCR/APSN 31RPCR-M029* Campylobacter coli C. coli Real-time PCR/APSN 31RPCR-M028* Campylobacter jejuni C. jejuni Real-time PCR/APSN 2012 Vet Food Agro Diagnostics (M) Sdn Bhd ( X) Page 6 of 11

7 31CPCR-M010 Listeria monocytogenes Listeria Conventional PCR/APSN 31RPCR-M022* Listeria monocytogenes Listeria Real-time PCR/APSN 31RPCR-M023* Listeria ivanovii - Real-time PCR/APSN 31RPCR-M024* Listeria innocua - Real-time PCR/APSN 31RPCR-M025* Listeria welshimeri - Real-time PCR/APSN 31RPCR-M026* Listeria seeligeri - Real-time PCR/APSN 31RPCR-M027* Listeria grayi - Real-time PCR/APSN 31RPCR-M060 Pasteurella multocida (Fowl cholera) - Real-time PCR/APSN 31RPCR-M049* Bacillus cereus - Real-time PCR/APSN 31RPCR-M045 Pseudomonas aeruginosa - Real-time PCR/APSN 31RPCR-M064* Yersinia enterocolitica - Real-time PCR/APSN I. RAPID DETECTION OF OTHER MICROBES (REAL-TIME PCR) Sample type: Animal tissues, organ, swab, food, feed, environmental samples TEST CODE 31RPCR-M040* 31RPCR-M041* 31RPCR-M042* DISEASE/PATHOGEN Aspergillus niger Aspergillus oryzae Aspergillus ustus TOXINS A. ELISA SCREENING TEST Sample type: Food, feed, raw food/feed materials TEST CODE TOXINS DESCRIPTION/BRAND 11ELSA-E010 Zearalenone Zea Screening ELISA/Romer Labs 11ELSA-E011 Total Aflatoxin Afla Screening ELISA/Romer Labs 11ELSA-E012 Deoxynivalenol DON Screening ELISA/Romer Labs 2012 Vet Food Agro Diagnostics (M) Sdn Bhd ( X) Page 7 of 11

8 TEST CODE TOXINS DESCRIPTION/BRAND 11ELSA-E015 Tricothenes T2 Screening ELISA/Romer Labs 11ELSA-E016 Ochratoxin Ochra Screening ELISA/Romer Labs 11ELSA-E017 Fumonisin Fumo Screening ELISA/Romer Labs B. LC-MS/MS CONFIRMATIVE TEST Sample type: Animal feed TEST CODE TOXINS DESCRIPTION 41LCMS-C022 Mycotoxins (Aflatoxin B1, T-2, Fumonisin B1, DON, Zearalenone & Ochratoxin A) 6-in-1 Mycotoxin test Aflatoxin B1& Ochratoxin A, LOD: 2ppb T-2, Fumonisin B1, DON & Zearalenone, LOD: 10ppb ANTIBIOTIC/CHEMICAL RESIDUES A. ELISA AND MICROBIOLOGICAL SCREENING TEST Sample type: Depends on the test TEST CODE ANTIBIOTIC/CHEMICAL RESIDUES DESCRIPTION 21BAAR-B012 Total Antibiotic Residues Screening Test (Meat & Milk) - Antibiotic screening test by microbiological assay 21BAAR-B044 Total Antibiotic Residues Screening Test (Egg & Swab) - Antibiotic screening test by microbiological assay 11ELSA-E033* Melamine-Wheat, Gluten and Pet Food Melamine Screening ELISA/Romer Labs B. LC-MS/MS CONFIRMATIVE TEST FOR ANTIBIOTIC/CHEMICAL RESIDUES Sample type: Animal tissues or feed (please contact the lab for other sample matrices) TEST CODE ANTIBIOTIC/CHEMICAL RESIDUES SAMPLE MATRICE 41LCMS-C018 Chloramphenicol (For meat and tissue) CAP Meat or tissue 2012 Vet Food Agro Diagnostics (M) Sdn Bhd ( X) Page 8 of 11

9 TEST CODE ANTIBIOTIC/CHEMICAL RESIDUES SAMPLE MATRICE 41LCMS-C069* Chloramphenicol (For feed) Feed 41LCMS-C019 Nitrofurans (AMOZ, AOZ, AHD, SC) Nitrofurans Meat or tissue 41LCMS-C020 Tetracyclines (TC, OTC, CTC, DC) Tetracyclines Meat or tissue 41LCMS-C021 Sulfonamides (Sulfadiazine, Sulfamethazine, Sulfonamides Meat or tissue Sulfadimethoxine, Sulphaquinoxaline) 41LCMS-C023 Fluoroquinolones (Difloxacin, Danofloxacin, Flumequine, Fluoroquinolones Meat or tissue Sarafloxacin, Ofloxacin & Enrofloxacin) 41LCMS-C024 Melamine - Feed 41LCMS-C025 Sudan Red (I, II, III and IV) - Egg yolk 41LCMS-C026 Malachite Green and Leucomalachite Green - Feed 41LCMS-C027* Clopidol - Meat or tissue MISCELLANEOUS TESTS TEST CODE OTHER TESTS DESCRIPTION/BRAND 41MOIS-C037* Moisture Analysis For feed, premixes 41PH00-C038 ph For water, wastewater, liquids 21BACI-B009* Sterility Test - 21AMC-B034* Freeze Drying - Bacteria - 21BAMC-B035 Bacteriological Water Quality Indicator For water, wastewater, liquids/apsn 21BAMC-B054* Cryopreservation of Bacteria and Yeast APSN Microcryobeads TM SUPPLIES PRODUCT CODE 81SAMP-P001* 81SAMP-P002* SUPPLIES PP Bottle Buffered Peptone Water 2012 Vet Food Agro Diagnostics (M) Sdn Bhd ( X) Page 9 of 11

10 PRODUCT CODE 81SAMP-P003* 81SAMP-P004* 81SAMP-P005* 81SAMP-P006* 81AGPL-P007* 81AGPL-P008* 81AGPL-P009* 81AGPL-P010* 81SAMP-P017* SUPPLIES Environmental Swab (Large) 8 inch/pc Environmental Swab (Small) 4 inch/pc Cotton Swabs (20pcs/pack) Bacteriological Water Quality Indicator Kit Standard Plate Count Agar Plate (SPCA) Total Coliform/E. coli Count Agar Plate (MA) Total Yeast and Mould Count Agar Plate (PDA) Pseudomonas Count Agar Plate (GSP) Sterile Inoculating Loops (20pcs/pack) 81APSN-P001* CytoStain Plus Stain 1 81APSN-P002* CytoStain Plus Stain 2 81APSN-P003* 81APSN-P004* 81APSN-P005* 81APSN-P006* 81APSN-P007* 81APSN-P008* Real Time PCR Test Kit* APSN 96-well Microplate U-bottom (Sterile) APSN 96-well Microplate U-bottom (Non-sterile) APSN 96-well Microplate Flat-bottom (Sterile) APSN 96-well Microplate Flat-bottom (Non-sterile) APSN Microcryobeads TM (6 vials/box) APSN Microcryobeads TM (100 vials/box) APSN Real-Time PCR Detection Test Kit (Halal Test, NDV, IBV, PCV, MG, MS, Avian Diseases, Porcine Diseases, Food pathogens, Aquaculture viruses, etc) GENERAL NOTES ON THE LABORATORY SERVICES: 1. The turnaround time for all tests is seven (7) working days EXCEPT for molecular assays (e.g. Real-Time PCR and PCR tests) and Bacterial Identification (up to species level) tests which is four (4) working days 2. Surcharge of 10% is applicable for very urgent results subject to availability USEFUL TECHNICAL NOTES: Please download from our website. VFAD2012-T101 Specimen Submission Guidelines for Serology Tests 2012 Vet Food Agro Diagnostics (M) Sdn Bhd ( X) Page 10 of 11

11 VFAD2012-T102 VFAD2012-T103 VFAD2012-T104 Specimen Submission Guidelines for Microbiology Tests Specimen Submission Guidelines for Molecular Biology Tests Specimen Submission Guidelines for Chemistry Tests Disclaimer: The abovementioned product/kit names and any registered and unregistered trademarks in this article are used merely for identification purposes only and remain the exclusive property of their respective owners Vet Food Agro Diagnostics (M) Sdn Bhd ( X) Page 11 of 11

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