TRACEABILITY and UNCERTAINTY
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1 ACP Acid phosphatase total 1-naphthyl phosphate NPP Acid phosphatase, non-prostatic 1-naphthyl phosphate (Inhib.:tartrate) ACP-P Acid phosphatase, prostatic 1-naphthyl phosphate (Inhib.:tartrate) ALB Albumin BCG plus ALB Albumin BCP ALP Alkaline phosphatase ALP Alkaline phosphatase ALP Alkaline phosphatase ALT Alanine aminotransferase IFCC with pyridoxalphosphate ALT Alanine aminotransferase IFCC without pyridoxal phosphate ALT Alanine aminotransferase IFCC without pyridoxal phosphate HiCo AMY-L Amylase alpha calculated ERM-DA70k/IFCC ERM-DA70k/IFCC (Tietz, 198), manual (Schumann, 2011), manual (2002) modified, manual (2002) modified, manual Roche reagent according to IFCC (1998), manual
2 AMY-L Amylase alpha STAT P-AMY Amylase alpha pancreatic EPS liquid AST Aspartate aminotransferase IFCC with pyridoxalphosphate AST Aspartate aminotransferase IFCC without pyridoxal phosphate BILD2 Bilirubin direkt Diazo Gen. 2 Jendrassik-Grof BILD2 Bilirubin direkt Diazo Gen. 2 Doumas BILT Bilirubin total Gen. BUN Blood urea nitrogen kinetic UV BUN Blood urea nitrogen kinetic UV STAT Ca o-cresolphthalein-complexone Ca o-cresolphthalein-complexone STAT Ca2 -Nitro- -methyl-bapta Gen Roche reagent according to IFCC (1998), manual (2002) modified, manual m m 712 acc. to Doumas m m m m m m 726 / 298 SRM 96c L 706 / 79 SRM 96c L 698 SRM 96c L2
3 Ca2 -Nitro- -methyl-bapta Gen. 2 STAT CHE Cholinesterase acethylthiocholine CHE Cholinesterase butyrylthiochoine Gen.2 CHOL Cholesterol total CHOD-PAP stand. ID/MS CHOL Cholesterol total CHOD-PAP stand. Abell-Kendall CK Creatine Kinase CO2-L Bicarbonat kinetic liquid CREA Creatinine plus CREA Creatinine Jaffé compensated STAT CREA Creatinine Jaffé rate-blanked and compensated Fe Iron Ferrozine SRM 96c L m m m m m Abell-Kendall 07 (aqueous volumetric standards prepared with pure Sodium Carbonate (ACS)) 2 / SRM 97
4 liquid stand. IFCC HiCo liquid stand. IFCC liquid stand. Szasz HiCo liquid stand. Szasz GLDH Glutamate Dehydrogenase GLU HK GLUH2 HK hemolysate Gen. 2 GLU HK STAT GLU GOD-PAP HBDH Hydroxybutyrate dehydrogenase alpha LAC Lactate enzymatic colorimetric test LDH Lactate dehydrogenase m m m m m Original formulation Persijn/v.d. Slik (1976), manual Original formulation Persijn/v.d. Slik (1976), manual (weighed in purified material)
5 LDH Lactate dehydrogenase with automatic sample predilution LDH Lactate dehydrogenase LIP Lipase enzymatic colorimetric test LIP Lipase enzymatic colorimetric test STAT Mg Magnesium xylidyl blue method Mg Magnesium xylidyl blue method STAT PHOS Phosphate (Inorganic) molybdate UV PHOS Phosphate (Inorganic) molybdate UV STAT TG Triglycerides GPO-PAP TG Triglycerides GPO-PAP GB TP Total protein Biuret Atomic absorption Atomic absorption m m m (NERL) (weighed in purified material) (NERL) (weighed in purified material) SRM 909b 678 SRM 927d
6 TP Total protein Biuret STAT UA Uric acid plus UIBC Unsaturated iron-binding capacity FerroZine UREA Urea kinetic UV UREA Urea kinetic UV STAT SRM 927d (iron) (weighed in purified material) m m m This uncertainty (expanded uncertainty; k = 2) was calculated in accordance with the Guide to the expression of uncertainty in (GUM: 199). For the estimation of the single standard uncertainties normal distribution of the results is assumed; the level of confidence of the expanded uncertainty is about 9% with the coverage factor k=2 (analogue to the 2sd of the standard uncertainty). 2 CRM = Certified, European SRM = Standard = Isotope Dilution - Mass Spectrometry IFCC = International Federationof clinical chemistry and laboratory medicine 6 ERM = European s 7 The value describes the uncertainty within a system class. By transferring the setpoint from one system class to another an additional systematic deviation can arise. This deviation is limited by the instrument to instrument specification. The relative maximum deviation is %, resp. Chol IDMS/AK 0.1.
TRACEABILITY and UNCERTAINTY
Cat. No. 0 79 0 90 ACP Acid phosphatase total -naphthyl phosphate NPP Acid phosphatase, non-prostatic -naphthyl phosphate (Inhib.:tartrate) ALB Albumin BCG plus ALB Albumin BCP ALP Alkaline phosphatase
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ATRYP Antitrypsin alpha 1 ERM-DA470k/IFCC 9 136 1.36 1.84 0.0184 GPROT Acid glycoprotein alpha 1 CRM 470 2 90.1 0.901 0.890 0.00890 ACP Acid phosphatase total 1-naphthyl phosphate 0.720 43.1 0.00703 0.421
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COBAS INTEGRA / cobas c - C.f.a.s. Cat. No. 0 79 30 90 Roche Diagnostics GmbH May 20 Acid phosphatase total -naphthyl phosphate Gen. 2 Integra 00 plus Acid phosphatase total -naphthyl phosphate Gen. 2
More informationTRACEABILITY and UNCERTAINTY 7
Routine Method COBAS INTEGRA systems Acid phosphatase total 1-naphthyl phosphate Integra 00 plus Acid phosphatase total 1-naphthyl phosphate Acid phosphatase, non-prostatic Integra 00 plus Acid phosphatase,
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AAT Antitrypsin alpha 1 AAGP Acid glycoprotein alpha 1 immunoturbidimetric Gen. Acid phosphatase total 1-naphthyl phosphate Gen. Acid phosphatase total 1-naphthyl phosphate Gen. Integra 00 plus Acid phosphatase,
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Roche Diagnostics GmbH Novembe 2012 Refeence Method Refeence Mateial Selected Measuement Pocedue Calibato Value Uncetainty 1 Unit ACP2 Acid phosphatase total 1-naphthyl phosphate Gen. 2 Roche eagent, manual
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Roche Diagnostics GmbH Novembe 2012 Refeence Method Refeence Mateial Selected Measuement Pocedue Assigned Value Uncetainty 1 Unit ACP2 Acid phosphatase total 1-naphthyl phosphate Gen.2 24.0 0.401 0.279
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Refeence Method Refeence Mateial Selected Measuement Acid phosphatase total 1-naphthyl phosphate Gen. 2 Intega 400 47.2 0.788 0.605 0.0101 Acid phosphatase total 1-naphthyl phosphate Gen. 2 Intega 800
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Roche Diagnostics GmbH Decembe 2012 Refeence Method Refeence Mateial Selected Measuement Pocedue Assigned Value Uncetainty 1 Unit Acid phosphatase total 1-naphthyl phosphate Gen. 2 Intega 400 20.4 0.341
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