doi: 1.138/nture862 humn hr. 21q MRPL39 murine Chr.16 Mrpl39 Dyrk1A Runx1 murine Chr. 17 ZNF295 Ets2 Znf295 murine Chr. 1 COL18A1 -/- lot: nti-dscr1 IgG hevy hin DSCR1 DSCR1 expression reltive to hevy hin 1..8.6.4.2 -/- 3 d 35 3 mrna reltive to GAPDH 2 1 ell ount (x 1 2 ) 25 2 15 1 e 5 VEGF (ng/ml): 1 norml humn ips tertoms Down syndrome ips tertoms hcd31 hcd31 + Hoehst hcd31 hcd31 + Hoehst Supplementl Figure 1. DSCR1 expression nd mouse nd humn Down syndrome models. () Comprison of humn hromosome (hr) 21 nd murine trisomy. The Down syndrome mouse model ontins the distl segment of murine hromosome 16, region of linkge onservtion with humn hromosome 21, nd the proximl end of murine hromosome 17. This hromosome onfers trisomy for most of the genes in the hromosome 16 segment. () DSCR1 protein expression is inresed 1.7-fold in the ererl ortex isolted from the mouse 1
doi: 1.138/nture862 model of Down syndrome s ompred to diploid littermtes. Cererl ortex from -/- mie ws proed s ontrol. DSCR1 expression is ompred reltive to kground IgG hevy hin nd. () Inresed mrna expression reltive to GAPDH in from mie s ompred to diploid littermtes s ssessed y qpcr. (d) VEGF-indued prolifertion of isolted from mie is signifintly inhiited s ompred to. Vlues re men ± sem. *p<.4. (e) Representtive imges of different tumors generted from indued pluripotent stem ells (ips) isolted from Down syndrome individuls nd from ytogenetilly norml helthy volunteers. Tumors re immunostined with humn speifi nti-cd31 (rrow) to demonstrte ips-derived mirovsulture. 2
doi: 1.138/nture862 5ʼ homology 1 2 3 mutnt Hprt lous pos.trl het het WT 5ʼ homology my- 1 2 3 NEO trgeting vetor my- PCR HAT seletion my- 1 2 3 4 trgeted funtionl Hprt lous genotyping d trnsgeni lot: nti-vegfr2 VEGFR2 trnsgeni lot: nti-my my-dscr1 mrna reltive to GAPDH 3. 2. 1. β-tin β-tin WT trnsgeni Supplementl Figure 2. Genertion of trnsgeni mouse with 3 opies of. () Sheme for trgeting My-epitope tgged opy of induile into the Hprt lous. Green rrows indite lotion of PCR primers. Numers denote exons. () PCR sreen of emryoni stem ells fter eletroportion of our My- onstrut. Pos. trl = positive ontrol, Het = heterozygous, WT=. () Western lot nlysis of primry isolted from trnsgeni nd littermtes with nti-vegfr2 ntiody (left) to verify endothelil ell identity nd n nti-my ntiody (right) to detet expression of the trgeted trnsgene. (d) RNA ws isolted from from (WT) nd trnsgeni mie nd treted with VEGF (5 ng/ml). mrna ws quntified y qpcr nlysis. 3
doi: 1.138/nture862 mouse.28% trnsgeni mouse H2-2f. 6.16% % CD31 + CD45 - ells.5.4.3.2.1 * CD45 1 1 1 1 2 1 3 1 4 1 11 1 11 2 1 1 3 11 4 4 CD31 WT Tg nti-cd31 FITC-letin merge + Hoehst trnsgeni tumor volume (mm 3 ) 2,5 2, 1,5 1, 5 Lewis lung rinom trnsgeni 15 18 21 23 27 29 31 33 37 4 43 46 dys Supplementl Figure 3. Anlysis of tumor ngiogenesis in trnsgeni nd mie. () Quntifition y flow ytometry of CD31-positive nd CD45-negtive ells isolted from tumors hrvested from nd trnsgeni mie. Representtive flow ytometry plots on left. Vlues re men ± sem. *p<.2. () Immunofluoresene nlysis of funtionl mirovessels in tumor setions hrvested from or trnsgeni mie fter intrvenous injetion of FITC-letin nd o-immunostining with nti-cd31. The mjority of mirovessels in tumors from mie demonstrted o-stining of FITC-letin nd CD31 (rrow heds) while tumors from trnsgeni mie hd CD31-retive mirovessels lking fluoresent letin o-stining (rrow) suggesting the sene of lumens in these CD31- positive endothelil sprouts. Br, 2 µm. () Growth of trnsplnted Lewis lung rinom fter inoultion with 1, tumor ells ws signifintly suppressed in trnsgeni mie s ompred to littermte ontrols. Vlues re men ± s.d., n=2-3, *p<.1. 4
doi: 1.138/nture862 +/- +/+ +/- +/+ -null FISH: proe = mouse hromosome 16 frgment genotyping WT humn murine DYRK1A β-tin Supplementl Figure 4. Anlysis of x +/- litters nd the ontriution of inresed expression of Dyrk1 to the nti-ngiogeni phenotype of trnsgeni. () Anlysis of kryotyping nd genotyping of litters otined from mting femles with +/- mles. Blood leukoytes were nlyzed using proe ginst region of hromosome 16. Nulei of lood leukoytes re stined y DAPI (lue) nd show red spots tht revel the presene of hromosomes 16 nd the segment deteted y FISH. () Representtive genotyping of litters. () Western lot nlysis of endogenous DYRK1A expression in (WT) humn nd murine. 5