Laboratory Millennium Special Pathology Laboratory, Office No. 10, 11, 12, First Floor, N.K. Image, Tilak Road, 2015 Sadashiv Peth, Pune, Maharashtra
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1 Last Amended on - Page 1 of 18 Specific Method Range of ing / I. CLINICAL BIOCHEMISTRY ENDOCRINOLOGY TESTS THYROID HORMONES 1. SERUM Triiodothyronine (T3) Chemiluminescence ng/ml SERUM Thyroxine (T4) Chemiluminescence ug/dl SERUM Thyroid stimulating hormone (TSH) 4. SERUM Free Triiodothyronine (FT3) Chemiluminescence uiu/ml 5.2 Chemiluminescence pg/ml SERUM Free Thyroxine (FT4) Chemiluminescence ng/dl 5.0 REPRODUCTIVE HORMONES 6. SERUM Follicle Stimulating Hormone (FSH) 7. SERUM Luteinizing Hormone (LH) Chemiluminescence miu/ml 3.6 Chemiluminescence miu/ml SERUM Prolactin Chemiluminescence ng/ml SERUM Estradiol Chemiluminescence pg/ml 6.6
2 Last Amended on - Page 2 of 18 Specific Method Range of ing / 10. SERUM Progesterone Chemiluminescence ng/ml SERUM Total osterone Chemiluminescence nmol/l SERUM Total β HCG Chemiluminescence miu/ml SERUM Free Beta HCG Chemiluminescence ng/ml SERUM Unconjugated Estriol Chemiluminescence ng/ml SERUM PAPP-A Chemiluminescence miu/ml SERUM Triple Marker (2 nd Trimester) ALFA Fetoprotein (AFP) Unconjugated Estriol Total β HCG 17. SERUM Double Marker s Pregnancy Associated Plasma Protein A (PAPP-A) Chemiluminescence+ PRISCA Software Chemiluminescence+ PRISCA Software Chemiluminescence+ PRISCA Software Chemiluminescence+ PRISCA Software ng/ml ng/ml miu/ml miu/ml 7.9
3 Last Amended on - Page 3 of 18 Specific Method Range of ing / Free Beta HCG Chemiluminescence+ PRISCA Software ng/ml SERUM Cortisol Chemiluminescence ug/dl SERUM Insulin Chemiluminescence mu/l SERUM Vitamin B 12 Chemiluminescence pg/ml SERUM Ferritin Chemiluminescence ng/ml SERUM Folic Acid Chemiluminescence ng/ml 9.4 TUMOR MARKERS 23. SERUM Alfa fetoprotein (AFP) Chemiluminescence ng/ml SERUM Carcinoembryonic antigen (CEA) 25. SERUM Total Prostate Specific Antigen (PSA) Chemiluminescence ng/ml 6.9 Chemiluminescence ng/ml SERUM CA 125 Chemiluminescence U/ml SERUM CA 19.9 Chemiluminescence U/ml SERUM 25-OH Vitamin D Chemiluminescence ng/ml 6.2
4 Last Amended on - Page 4 of 18 Specific Method Range of ing / BIOCHEMISTRY ROUTINE 29. FLUORIDE PLASMA Glucose Glucose Oxidase (Trinder's method) mg/dl SERUM Urea Urease End Point mg/dl SERUM Creatinine Jaffe Reaction mg/dl SERUM Cholesterol CHOD-PAP mg/dl SERUM Triglycerides GPO Method mg/dl SERUM HDL Cholesterol PVS/PE GME Method mg/dl SERUM Total Cholesterol/ Hdl Cholesterol Ratio Calculation 36. SERUM VLDL Cholesterol Calculation 37. SERUM LDL Cholesterol Calculation 38. SERUM Serum Glutamate Pyruvate Transaminase (SGPT) 39. SERUM Serum Glutamate Oxaloacetate Transaminase (SGOT) IFCC without P5P U/L 11.9 IFCC without P5P U/L 8.2
5 Last Amended on - Page 5 of 18 Specific Method Range of ing / 40. SERUM Albumin Bromocresol Green 1 6 g/dl SERUM Total Protein Biuret End Point Method g/dl SERUM Total Bilirubin Diazo Method mg/dl SERUM Direct Bilirubin Diazo Method mg/dl SERUM Uric Acid Uricase/POD mg/dl SERUM Calcium Modified Arsenazo Method 0 14 mg/dl SERUM Phosphorus Phosphomolyb Formation mg/dl SERUM Amylase CNPG U/L SERUM Magnesium Xylidyl blue with ATCS mg/dl SERUM Alkaline Phosphatase p-nitrophenyl phosphate AMP buffer U/L SERUM Lipase Methyl resorufin U/L WHOLE BLOOD HbA1c HPLC % SERUM Sodium Ion-Selective Electrode (Direct) mmol/l 3.4
6 Last Amended on - Page 6 of 18 Specific Method Range of ing / 53. SERUM Potassium Ion-Selective Electrode (Direct) 54. SERUM Chloride Modified Thiocynate Method mmol/l mmol/l 3.5 II. CLINICAL PATHOLOGY 1. URINE Physical Examination Volume Visual Measured in ml Appearance Visual Clear, Slightly hazy, Hazy Colour Visual Colourless, Pale yellow, yellow, Dark Yellow, Orange, Red etc. Chemical Examination Glucose Glucose Oxidase, Peroxidase Method Present/Absent. If present Trace to 4+ Bilirubin Diazotized Dichloroaniline Present/Absent. If present 1+ to 3+ Ketone Sodium- Nitroprusside Method Present/Absent. If present Trace to 4+
7 Last Amended on - Page 7 of 18 Specific Method Range of ing / Protein Tetrabromphenol Method Present/Absent. If present Trace to 4+ ph Double Indicator Procedure Interpreted as acidic, neutral or alkaline Urobilinogen Ehrlich Reaction Present/Absent. If present Trace to 3+ Specific Gravity Ionic Concentration to Blood Diisopropylbenzene Dihydroperoxide and Tetramethylbenzidine Method Present/Absent. If present Trace to 3+ Urine - Microscopy Microscopic Observation Types Of Cells, Number, Casts, Crystals, Parasites Etc 2. STOOL Physical Examination Colour Visual Examination Brownish, yellowish, greenish etc Consistency Visual Examination Liquid, semi liquid, semi solid, Solid Blood Visual Examination Present / Absent
8 Last Amended on - Page 8 of 18 Specific Method Range of ing / Mucus Visual Examination Present / Absent Chemical Examination Occult Blood Non-Benzidine Method Present/Absent. If present Trace-4+ Microscopic Microscopy by Lugol s iodine Cysts, ova, Type of cells 3. RANDOM URINE Pregnancy test Rapid Chromatographic Immunoassay Interpreted as positive or negative 4. RANDOM URINE Drugs of Abuse (Amphetamine, Benzodiazepine, Barbiturate, Cocaine, Marijuana, Morphine) Competitive binding immunoassay Interpreted as positive or negative III. HAEMATOLOGY & IMMUNOHAEMATOLOGY 1. EDTA BLOOD Hemoglobin Non-cyanide Hemoglobin method g/dl EDTA BLOOD Total WBC Count DC Detection Method x 10 3 /ul EDTA BLOOD RBC Count DC Detection Method x 10 6 /ul EDTA BLOOD Platelet Count Impedence Measurement x 10 3 /ul 6.8
9 Last Amended on - Page 9 of 18 Specific Method Range of ing / 5. EDTA BLOOD Mean Corpuscular volume (MCV) 6. EDTA BLOOD Mean corpuscular Hemoglobin (MCH) 7. EDTA BLOOD Mean Corpuscular Hemoglobin Concentration (MCHC) Calculated fl 3.0 Calculated pg 4.5 Calculated 32-37% EDTA BLOOD Packed cell volume Calculated g/dl EDTA BLOOD Peripheral smear study Microscopy of stained smear Morphology of WBC, RBC, Platelets and presence or absence of parasites 10. CITRATE BLOOD Erythrocyte sedimentation rate Westergren Method mm/hr 11. EDTA BLOOD AND CLOT Blood Group Tube Agglutination And Slide Method Interpreted as A, B, AB, O blood groups and Rh factor as Positive or Negative 12. EDTA BLOOD Absolute Eosinophil Count Microscopy + Impedence Measurement
10 Last Amended on - Page 10 of 18 Specific Method Range of ing / 13. EDTA BLOOD Malaria Parasite Microscopy Interpreted as detected (P. falciparum or P. vivax) or not detected 14. EDTA BLOOD Reticulocyte Count Microscopy of Stained Smear (Brilliant Cresyl Blue) 0-100% EDTA BLOOD Hemoglobin Variants Hb A2 Hb F IMMUNO HEMATOLOGY HIV MONITORING TEST High Performance Liquid Chromatography High Performance Liquid Chromatography 0-100% % EDTA BLOOD Absolute CD4 Flow Cytometry / Impedence Measurement 17. EDTA BLOOD Absolute CD4+CD8 Flow Cytometry / Impedence Measurement cells/ul cells/ul 5.3 LEUKEMIA IMMUNOPHENOTYPING 18. EDTA BLOOD, CD3 Flow Cytometry Interpreted as Negative
11 Last Amended on - Page 11 of 18 Specific Method Range of ing / 19. EDTA BLOOD, 20. EDTA BLOOD, 21. EDTA BLOOD, 22. EDTA BLOOD, 23. EDTA BLOOD, 24. EDTA BLOOD, CD4 Flow Cytometry Interpreted as Negative CD8 Flow Cytometry Interpreted as Negative CD5 Flow Cytometry Interpreted as Negative CD7 Flow Cytometry Interpreted as Negative CD10 Flow Cytometry Interpreted as Negative CD19 Flow Cytometry Interpreted as Negative
12 Last Amended on - Page 12 of 18 Specific Method Range of ing / 25. EDTA BLOOD, 26. EDTA BLOOD, 27. EDTA BLOOD, 28. EDTA BLOOD, 29. EDTA BLOOD, 30. EDTA BLOOD, CD10/ CD 19 DUAL Flow Cytometry Interpreted as Negative CD20 Flow Cytometry Interpreted as Negative CD 117 Flow Cytometry Interpreted as Negative CD 64 Flow Cytometry Interpreted as Negative CD13 Flow Cytometry Interpreted as Negative CD14 Flow Cytometry Interpreted as Negative
13 Last Amended on - Page 13 of 18 Specific Method Range of ing / 31. EDTA BLOOD, 32. EDTA BLOOD, 33. EDTA BLOOD, 34. EDTA BLOOD, 35. EDTA BLOOD, 36. EDTA BLOOD, CD15 Flow Cytometry Interpreted as Negative CD33 Flow Cytometry Interpreted as Negative MPO Flow Cytometry Interpreted as Negative CD 41 Flow Cytometry Interpreted as Negative HLA DR Flow Cytometry Interpreted as Negative CD34 Flow Cytometry Interpreted as Negative
14 Last Amended on - Page 14 of 18 Specific Method Range of ing / 37. EDTA BLOOD, 38. EDTA BLOOD, 39. EDTA BLOOD, 40. EDTA BLOOD, 41. EDTA BLOOD, 42. EDTA BLOOD, Anti AMPO Flow Cytometry Interpreted as Negative FMC7 Flow Cytometry Interpreted as Negative CD 23 Flow Cytometry Interpreted as Negative CD 38 Flow Cytometry Interpreted as Negative Anti kappa Flow Cytometry Interpreted as Negative Anti lambda Flow Cytometry Interpreted as Negative
15 Last Amended on - Page 15 of 18 Specific Method Range of ing / 43. EDTA BLOOD, Anti TDT Flow Cytometry Interpreted as Negative 44. MARROW Bone Marrow Aspiration Report Microscopic Examination IV. MICROBIOLOGY & SEROLOGY 1. BLOOD, BODY FLUIDS, TISSUE, PUS MTB PCR (MTC) Real Time Polymerase Chain Reaction Detectable/Not Detectable 2. BLOOD HIV 1 Quantitative PCR Real Time Polymerase Chain Reaction IU/ml 8.2 TORCH GROUP 3. SERUM Toxoplasma IgM Chemiluminescence Interpreted as positive or with index 4. SERUM Toxoplasma IgG Chemiluminescence Interpreted as positive or with index 5. SERUM Rubella IgM Chemiluminescence Interpreted as positive or with index
16 Last Amended on - Page 16 of 18 Specific Method Range of ing / 6. SERUM Rubella IgG Chemiluminescence Interpreted as positive or with index 7. SERUM CMV IgM Chemiluminescence Interpreted as positive or with index 8. SERUM CMV IgG Chemiluminescence Interpreted as positive or with index SERUM Herpes Simplex IgM I & II Enzyme Linked Immunosorbent Assay Interpreted as positive or SERUM Herpes Simplex IgG I & II Enzyme Linked Immunosorbent Assay Interpreted as positive or SERUM Dengue IgG / IgM Rapid Interpreted as positive or negative 12. SERUM Rheumatoid Arthritis Factor (RA) Slide Agglutination Interpreted as absolute value. Linear Upto 256 IU/mL. If positive titre is mentioned
17 Last Amended on - Page 17 of 18 Specific Method Range of ing / 13. SERUM C-Reactive Protein Slide Agglutination Interpreted as absolute value. Linear Upto 6 mg/l. If positive titre is mentioned AUTOIMMUNE TESTS 14. SERUM Anti Nuclear Antibody (A) Enzyme Linked Immunosorbent Assay Interpreted as positive or negative 10.8 VIRAL MARKERS HEPATITIS MARKERS 15. SERUM Hepatitis B surface antigen (Hbs Ag) Enzyme Linked Immunosorbent Assay Interpreted as positive or SERUM Anti HAV IgM Chemiluminescence Interpreted as positive or 17. SERUM Anti HBc IgM Chemiluminescence Interpreted as positive or SERUM Hepatitis B Envelope Antigen (Hbe Ag) Chemiluminescence Interpreted as positive or 7.3
18 Last Amended on - Page 18 of 18 Specific Method Range of ing / 19. SERUM Anti HBc Total Chemiluminescence Interpreted as positive or 20. SERUM Anti Hbe Chemiluminescence Interpreted as positive or 21. SERUM Anti HBs Total Chemiluminescence Interpreted as positive or SERUM Anti HEV IgM Enzyme Linked Immunosorbent Assay Interpreted as positive or SERUM Anti HCV Tridot Method Interpreted as positive or negative 24. SERUM HCV ELISA Enzyme Linked Immunosorbent Assay Interpreted as positive or SERUM Western Blot for HIV I and HIV II Blot Method Interpreted as Detected or not detected as per CDC criteria -X-X-X-X-X-X-X-X-X-X-X-X-X-X-X-X-
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