Drugs of Abuse Confirmation/Quantitation - Opiates - Urine CDCO OPI 211

Transcription

1 ARUP Standard Transport Tube. (Min: 2 ml) Unacceptable Conditions: Separator tubes. Plasma or whole blood collected in lt. blue (sodium citrate). Specimens exposed to repeated freeze/thaw cycles. Stability: After separation from cells: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 3 years CPT Code(s): Drugs of Abuse Confirmation/Quantitation - Cocaine Metabolite (Benzoylecgonine) - Urine CDCO COCA Methodology: Quantitative Gas Chromatography-Mass Spectrometry Specimen Required: Collect: Random urine. Specimen Preparation: Transfer 4 ml urine with no additives or preservatives to an ARUP Standard Transport Tube. (Min: 2 ml) Storage/Transport Temperature: Room temperature. Unacceptable Conditions: Specimens exposed to repeated freeze/thaw cycles. Stability: Ambient: 1 week; Refrigerated: 1 month; Frozen: 3 years CPT Code(s): Drugs of Abuse Confirmation/Quantitation - Fentanyl & Metabolite - Serum or Plasma CDCO FENSP Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry Specimen Required: Collect: Gray (sodium fluoride/potassium oxalate). Also acceptable: Plain red, green (sodium heparin), lavender (EDTA), or pink (K 2 EDTA). Specimen Preparation: Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 4 ml serum or plasma to an ARUP Standard Transport Tube. (Min: 2 ml) Unacceptable Conditions: Separator tubes. Plasma or whole blood collected in lt. blue (sodium citrate). Specimens exposed to repeated freeze/thaw cycles. Stability: After separation from cells: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 3 years CPT Code(s): Drugs of Abuse Confirmation/Quantitation - Fentanyl & Metabolite - Urine CDCO FENU Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry Specimen Required: Collect: Random urine. Specimen Preparation: Transfer 4 ml urine with no additives or preservatives to an ARUP Standard Transport Tube. (Min: 0.5 ml) Storage/Transport Temperature: Room temperature. Unacceptable Conditions: Specimens exposed to repeated freeze/thaw cycles. Stability: Ambient: 1 month; Refrigerated: 1 month; Frozen: 9 months CPT Code(s): Drugs of Abuse Confirmation/Quantitation - Methadone & Metabolite - Meconium CONFMTD M Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry Specimen Required: Collect: All meconium (blackish material) excreted until milk/formula based stool (yellow-green) appears. Specimen Preparation: Transport 1 g (equivalent to 1/2 inch cube) for each separate confirmation required. (Min: 0.5 g or 1/4 inch cube) Storage/Transport Temperature: Room temperature. Stability: Ambient: 1 week; Refrigerated: 3 months; Frozen: 1 year CPT Code(s): Drugs of Abuse Confirmation/Quantitation - Methadone & Metabolite - Serum or Plasma METHADO SP Methodology: Quantitative Liquid Chromatography-Tandem Mass Specimen Required: Collect: Gray (sodium fluoride/potassium oxalate). Also acceptable: Plain red, green (sodium heparin), lavender (EDTA), or pink (K 2 EDTA). Specimen Preparation: Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 4 ml serum or plasma to an ARUP Standard Transport Tube. (Min: 2 ml) Unacceptable Conditions: Separator tubes. Plasma or whole blood collected in lt. blue (sodium citrate). Specimens exposed to repeated freeze/thaw cycles. Stability: After separation from cells: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 3 years CPT Code(s): Drugs of Abuse Confirmation/Quantitation - Opiates - Meconium CONFOPI M Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry Specimen Required: Collect: All meconium (blackish material) excreted until milk/formula based stool (yellow-green) appears. Specimen Preparation: Transport 1 g (equivalent to 1/2 inch cube) for each separate confirmation required. (Min: 0.5 g or 1/4 inch cube) Storage/Transport Temperature: Room temperature. Stability: Ambient: 1 week; Refrigerated: 3 months; Frozen: 1 year CPT Code(s): Drugs of Abuse Confirmation/Quantitation - Opiates - Serum or Plasma OPIS SP Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry Specimen Required: Collect: Gray (sodium fluoride/potassium oxalate). Also acceptable: Plain red, green (sodium heparin), lavender (EDTA), or pink (K 2 EDTA). Specimen Preparation: Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 4 ml serum or plasma to an ARUP Standard Transport Tube. (Min: 2 ml) Unacceptable Conditions: Separator tubes. Plasma or whole blood collected in lt. blue (sodium citrate). Specimens exposed to repeated freeze/thaw cycles. Stability: After separation from cells: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 3 years CPT Code(s): Drugs of Abuse Confirmation/Quantitation - Opiates - Urine CDCO OPI 211

2 Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry Specimen Required: Collect: Random urine. Specimen Preparation: Transfer 4 ml with no additives or preservatives urine to an ARUP Standard Transport Tube. (Min: 1 ml) Storage/Transport Temperature: Room temperature. Unacceptable Conditions: Specimens exposed to repeated freeze/thaw cycles. Stability: Ambient: 1 week; Refrigerated: 1 month; Frozen: 3 years CPT Code(s): Drugs of Abuse Confirmation/Quantitation - Phencyclidine (PCP) - Meconium CONFPCP M Methodology: Quantitative Gas Chromatography-Mass Spectrometry Specimen Required: Collect: All meconium (blackish material) excreted until milk/formula based stool (yellow-green) appears. Specimen Preparation: Transport 1 g (equivalent to 1/2 inch cube) for each separate confirmation required. (Min: 0.5 g or 1/4 inch cube) Storage/Transport Temperature: Room temperature. Stability: Ambient: 1 week; Refrigerated: 3 months; Frozen: 1 year CPT Code(s): Drugs of Abuse Confirmation/Quantitation - Phencyclidine (PCP) - Urine CDCO PCP Methodology: Quantitative Gas Chromatography-Mass Spectrometry Specimen Required: Collect: Random urine. Specimen Preparation: Transport 10 ml urine. (Min: 2 ml) Storage/Transport Temperature: Room temperature. Unacceptable Conditions: Specimens exposed to repeated freeze/thaw cycles. Stability: Ambient: 1 week; Refrigerated: 1 month; Frozen: 3 years CPT Code(s): Drugs of Abuse Confirmation/Quantitation - Phencyclidine - Serum or Plasma PHENCYCLID Methodology: Quantitative Gas Chromatography-Mass Spectrometry Specimen Required: Collect: Gray (sodium fluoride/potassium oxalate). Also acceptable: Plain red, green (sodium heparin), lavender (EDTA), or pink (K 2 EDTA). Specimen Preparation: Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 4 ml serum or plasma to an ARUP Standard Transport Tube. (Min: 2 ml) Unacceptable Conditions: Separator tubes. Plasma or whole blood collected in lt. blue (sodium citrate). Specimens exposed to repeated freeze/thaw cycles. Stability: After separation from cells: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 3 years CPT Code(s): Drugs of Abuse Confirmation/Quantitation - Propoxyphene & Metabolite - Meconium CONFPPXY M Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry Specimen Required: Collect: All meconium (blackish material) excreted until milk/formula based stool (yellow-green) appears. Specimen Preparation: Transport 1 g (equivalent to 1/2 inch cube) for each separate confirmation required. (Min: 0.5 g or 1/4 inch cube) Storage/Transport Temperature: Room temperature. Stability: Ambient: 1 week; Refrigerated: 3 months; Frozen: 1 year CPT Code(s): Drugs of Abuse Confirmation/Quantitation - Propoxyphene & Metabolite - Serum or Plasma PROPOXY SP Methodology: Quantitative Liquid Chromatography-Tandem Mass Specimen Required: Collect: Gray (sodium fluoride/potassium oxalate). Also acceptable: Plain red, green (sodium heparin), lavender (EDTA), or pink (K 2 EDTA). Specimen Preparation: Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 4 ml serum or plasma to an ARUP Standard Transport Tube. (Min: 2 ml) Unacceptable Conditions: Separator tubes. Plasma or whole blood collected in lt. blue (sodium citrate). Specimens exposed to repeated freeze/thaw cycles. Stability: After separation from cells: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 3 years CPT Code(s): Drugs of Abuse Confirmation/Quantitation - Propoxyphene & Metabolite - Urine CDCO PPXY Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry Specimen Required: Collect: Random urine. Specimen Preparation: Transfer 4 ml with no additives or preservatives urine to an ARUP Standard Transport Tube. (Min: 1 ml) Storage/Transport Temperature: Room temperature. Unacceptable Conditions: Specimens exposed to repeated freeze/thaw cycles. Stability: Ambient: 1 week; Refrigerated: 1 month; Frozen: 3 years CPT Code(s): Drugs of Abuse Confirmation/Quantitation - Amphetamine - Serum or Plasma AMPHETAM Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry Specimen Required: Collect: Gray (sodium fluoride/potassium oxalate). Also acceptable: Plain red, green (sodium heparin), lavender (EDTA), or pink (K 2 EDTA). Specimen Preparation: Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 2 ml serum or plasma to an ARUP Standard Transport Tube. (Min: 1 ml) Unacceptable Conditions: Separator tubes. Plasma or whole blood collected in lt. blue (sodium citrate). Specimens exposed to repeated freeze/thaw cycles. Stability: After separation from cells: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 3 years CPT Code(s):

3 Drugs of Abuse Panel, Meconium - Screen with Reflex to Confirmation/Quantitation MEC 9 Methodology: Qualitative Enzyme-Linked Immunsorbent Assay/ Quantitative Gas Chromatography-Mass Spectrometry/Quantitative Liquid Chromatography-Tandem Mass Spectrometry Specimen Required: Collect: Meconium. All meconium (blackish material) excreted until milk/formula based stool (yellow-green) appears. Storage/Transport Temperature: All meconium available (4 g is preferred) at C. (Min: 2 g or 3/4 inch cube on each side) Stability: Ambient: 1 week; Refrigerated: 3 months; Frozen: 1 year CPT Code(s): x9 Screen; if positive add appropriate CPT code for drug confirmed: Marijuana; Cocaine; Opiates; Phencyclidine; Amphetamines; Barbiturates; Methadone; Benzodiazepine; Propoxyphene Drugs of Abuse Screen - Cocaine & Metabolites - Urine U COC Methodology: Enzyme Immunoassay Specimen Required: Collect: Random urine. Specimen Preparation: Mix well. Transfer 4 ml urine to an ARUP Standard Transport Tube. (Min: 1 ml) Also acceptable: Room temperature or frozen. Stability: Ambient: 1 week; Refrigerated: 1 month; Frozen: 3 years CPT Code(s): Drugs of Abuse Screen - Opiates - Urine U OPI Methodology: Enzyme Immunoassay Specimen Required: Collect: Random urine. Specimen Preparation: Mix well. Transfer 4 ml urine to an ARUP Standard Transport Tube. (Min: 1 ml) Also acceptable: Room temperature or frozen. Stability: Ambient: 1 week; Refrigerated: 1 month; Frozen: 3 years CPT Code(s): Drugs of Abuse Screen - Stimulant Amines - Urine U AMP Methodology: Enzyme Immunoassay Specimen Required: Collect: Random urine. Specimen Preparation: Mix well. Transfer 4 ml urine to an ARUP Standard Transport Tube. (Min: 1 ml) Also acceptable: Room temperature or refrigerated. Unacceptable Conditions: Specimens exposed to repeated freeze/thaw cycles. Stability: Ambient: 1 week; Refrigerated: 1 month; Frozen: 3 years CPT Code(s): Drugs of Abuse Test, Alcohol, Urine - Screen with Reflex to Confirmation/Quantitation CDASUALC Methodology: Semi-Quantitative Alcohol Dehydrogenase/ Qualitative Gas Chromatography-Flame Ionization Detection Specimen Required: Collect: Random urine. Specimen Preparation: Transport 10 ml urine. (Min: 2 ml) Newborn minimum: 1 ml for the initial test and 1 ml for the confirmation of screen positive. Storage/Transport Temperature: Room temperature. Remarks: If applicable, indicate NEWBORN prominently on the test request form. Unacceptable Conditions: Specimens exposed to repeated freeze/thaw cycles. Stability: Ambient: 1 week; Refrigerated: 1 month; Frozen: 3 years CPT Code(s): Drug screen; if positive, add Confirmation Dyazide, Serum or Plasma DYAZID Methodology: High Performance Liquid Chromatography Specimen Required: Collect: Plain red, gray (sodium fluoride/potassium oxalate), green (sodium heparin), or lavender (EDTA). Specimen Preparation: Transfer 4 ml serum or plasma to an ARUP Standard Transport Tube. (Min: 1 ml) Storage/Transport Temperature: Room temperature. Unacceptable Conditions: Serum separator tubes. Stability: No stabilities available at this time. CPT Code(s): x Dyazide, Urine DYAZIDE UR Methodology: High Performance Liquid Chromatography Specimen Required: Collect: Random urine. Specimen Preparation: Transfer 4 ml urine to an ARUP Standard Transport Tube. (Min: 1 ml) Storage/Transport Temperature: Room temperature. Stability: No stabilities available at this time. CPT Code(s): x Dyphylline Quantitative, Serum or Plasma DYPHYLLIN Methodology: High Performance Liquid Chromatography Specimen Required: Collect: Plain red or lavender (EDTA). Specimen Preparation: Transfer 2 ml serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 ml) Storage/Transport Temperature: Room temperature. Unacceptable Conditions: Serum separator tubes. Stability: Ambient: 2 weeks; Refrigerated: 2 weeks; Frozen: 2 weeks CPT Code(s): Dyphylline Quantitative, Urine DYPHYLLI U Methodology: High Performance Liquid Chromatography Specimen Required: Collect: Random urine. Specimen Preparation: Transfer 1 ml urine to an ARUP Standard Transport Tube. (Min: 0.5 ml) Stability: Ambient: 1 month; Refrigerated: 1 month; Frozen: 1 month CPT Code(s):

4 Dysautonomia, Familial (IKBKAP) 2 Mutations IKBKAP Methodology: Polymerase Chain Reaction/ASPE Bead Array Specimen Required: Collect: Lavender (EDTA), pink (K 2 EDTA), or yellow (ACD Solution A or B). Specimen Preparation: Transport 3 ml whole blood. (Min: 1 ml) CPT Code(s): Isolation; x2 Enzymatic digestion; Amplification; x2 Mutation identification; Separation and identification; Interpretation and report - Additional CPT code modifiers may be required for procedures performed to test for oncologic or inherited disorders Dysautonomia, Familial (IKBKAP) 2 Mutations, Fetal IKBKAP FE Methodology: Polymerase Chain Reaction/ASPE Bead Array Specimen Required: Collect: Fetal Specimen: Two T-25 flasks at 80% confluency of cultured amniocytes. If the client is unable to culture amniocytes, this can be arranged by contacting ARUP Client Services at (800) Or amniotic fluid. AND Maternal Cell Contamination Specimen: Lavender (EDTA), pink (K 2 EDTA), or yellow (ACD Solution A or B). Specimen Preparation: Cultured Amniocytes: Fill flasks with culture media. Transport two T-25 flasks at 80% confluency of cultured amniocytes. Backup cultures must be retained at the client's institution until testing is complete. OR Amniotic Fluid: Transport 10 ml unspun fluid. (Min: 5 ml) AND Maternal Cell Contamination Specimen: Transport 3 ml whole blood. (Min: 1 ml) Storage/Transport Temperature: Cultured Amniocytes: CRITICAL ROOM TEMPERATURE. Must be received within 48 hours of shipment due to liability of cells. Amniotic fluid: Room temperature. Maternal Cell Contamination Specimen: Room temperature. Remarks: Maternal specimen is recommended for proper test interpretation. Order Maternal Cell Contamination. Patient History Form is available on the ARUP Web site or by contacting ARUP Client Services. Stability: Fetal Specimen: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable Maternal Cell Contamination Specimen: Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable CPT Code(s): Isolation; x2 Digestion; Amplification; x2 Extension; Capillary electrophoresis; Interpretation and report. FCC: Multiplex amplification (first two sequences); x14 Additional amplification; Capillary electrophoresis. If MCC MAT ( ) is performed: Isolation; Multiplex amplification; x14 Additional amplification; Capillary electrophoresis; Interpretation and report - Additional CPT code modifiers may be required for procedures performed to test for oncologic or inherited disorders E-Cadherin by Immunohistochemistry ECADH IHC Methodology: Immunohistochemistry Specimen Required: Collect: Tissue or cells. Specimen Preparation: Formalin fix (10% neutral buffered formalin is preferred) and paraffin embed specimen (Cells must be prepared into a cellblock). If sending precut slides, do not oven bake. Protect paraffin block and/or slides from excessive heat. Ship in cooled container during summer months. Storage/Transport Temperature: Room temperature or refrigerated. Remarks: IMMUNOHISTOCHEMISTRY ORDERING AND SUBMISSION DETAILS: Submit electronic request. If you do not have electronic ordering capability, use an ARUP Immunohistochemistry Stain Form (#32978) with an ARUP client number. For additional technical details, please contact ARUP Client Services. Unacceptable Conditions: Depleted specimens. Specimens submitted with non-representative tissue type. Stability: Ambient: Indefinite; Refrigerated: Indefinite; Frozen: Indefinite CPT Code(s): E. coli Shiga-like Toxin by EIA ECSLT AG Methodology: Qualitative Enzyme Immunoassay Specimen Required: Collect: Stool. OR actively growing isolate of E. coli, in pure culture or on a swab in bacterial transport media. Specimen Preparation: Stool: Place in enteric transport media (Cary-Blair) (ARUP Supply #29799) immediately after collection. Available online through esupply using ARUP Connect or contact ARUP Client Services at (800) (Min: 1 g) OR E. coli Isolate: Transfer to a sealed container. Storage/Transport Temperature: Stool: Refrigerated. E. coli Isolate: Room temperature. Submit specimen according to Biological Substance, Category B, shipping guidelines. Unacceptable Conditions: Specimens or isolates from sources other than stool. Specimens in preservatives. Stability: Stool: Ambient: 24 hours; Refrigerated: 72 hours; Frozen: 1 week E. coli Isolate: Ambient: 1 week; Refrigerated: Unacceptable; Frozen: Unacceptable CPT Code(s): Ear Culture MC EAR Methodology: Standard reference procedures for aerobic bacterial culture and identification Specimen Required: Collect: Aspirate or drainage on swab. Specimen Preparation: Aspirate: Transfer to a sterile container. OR transport Sterile Syringe. Storage/Transport Temperature: Room temperature. Submit specimen according to Biological Substance, Category B, shipping guidelines. Unacceptable Conditions: Swabs of external ear canal without drainage. Dry swabs. Stability: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable CPT Code(s): Eastern Equine Encephalitis Antibody, IgG by IFA, CSF EAST G CSF Methodology: Semi-Quantitative Indirect Fluorescent Antibody Specimen Required: Collect: CSF. Specimen Preparation: Transfer 2 ml CSF to an ARUP Standard Transport Tube. (Min: 0.15 ml) Unacceptable Conditions: Contaminated, heat-inactivated, or hemolyzed specimens. 214

5 Stability: Ambient: 8 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) CPT Code(s): Eastern Equine Encephalitis Antibody, IgG by IFA, Serum EASTERN Methodology: Semi-Quantitative Indirect Fluorescent Antibody Transport Tube. (Min: 0.05 ml) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent. Unacceptable Conditions: Severely contaminated, hemolyzed, or severely lipemic specimens. CPT Code(s): Eastern Equine Encephalitis Antibody, IgM by IFA, CSF EAST M CSF Methodology: Semi-Quantitative Indirect Fluorescent Antibody Specimen Required: Collect: CSF. Specimen Preparation: Transfer 2 ml CSF to an ARUP Standard Transport Tube. (Min: 0.15 ml) Unacceptable Conditions: Contaminated, heat-inactivated, or hemolyzed specimens. Stability: Ambient: 8 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) CPT Code(s): Eastern Equine Encephalitis Antibody, IgM by IFA, Serum EAST M SER Methodology: Semi-Quantitative Indirect Fluorescent Antibody Specimen Preparation: Transfer 1 ml serum to an ARUP Standard Transport Tube. (Min: 0.15 ml) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent. Unacceptable Conditions: Plasma. Contaminated, heat-inactivated, hemolyzed, or severely lipemic or specimens. CPT Code(s): Echinococcus Antibody, IgG ECHINO Methodology: Semi-Quantitative Enzyme-Linked Immunosorbent Assay Transport Tube. (Min: 0.15 ml) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of acute specimens. Mark specimens plainly as acute or convalescent. Unacceptable Conditions: Contaminated or severely lipemic specimens. CPT Code(s): Echovirus Antibodies ECHO Methodology: Semi-Quantitative Serum Neutralization Assay Specimen Required: Collect: Serum separator tube or plain red. Also acceptable: CSF. Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transport 3 ml serum or CSF. (Min: 0.3 ml) Parallel testing is preferred and convalescent samples must be received within 30 days from receipt of acute samples. Mark samples plainly as "acute" or "convalescent." Unacceptable Conditions: Plasma. Stability: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year CPT Code(s): x5 Echovirus (Types 6, 7, 9, 11, and 30) EGFR Gene Amplification by FISH EGFRFISH Methodology: Fluorescence in situ Hybridization Specimen Required: Collect: Tumor tissue. Specimen Preparation: Formalin fix (10% neutral buffered formalin) and paraffin embed tissue. Transport tissue block or 5 unstained 5 micron slides. (Min: 3 slides) Protect paraffin block from excessive heat. Storage/Transport Temperature: Room temperature or refrigerated. Ship in cooled container during summer months. Remarks: Include surgical pathology report. Unacceptable Conditions: Frozen specimens. Specimens fixed or processed in alternative fixatives (alcohol, Prefer ) or heavy metal fixatives (B-4 or B-5). No tumor in tissue. Decalcified specimens. Stability: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable CPT Code(s): x EGFR Mutation Detection by PCR and Fragment Analysis EGFR PCR Methodology: Polymerase Chain Reaction/Fragment Analysis Specimen Required: Collect: Tumor tissue. Specimen Preparation: Formalin fix (10% neutral buffered formalin) and paraffin embed tissue. Transport tissue block or 5 unstained 5 micron slides. (Min: 3 slides) Protect paraffin block from excessive heat. Storage/Transport Temperature: Room temperature or refrigerated. Ship in cooled container during summer months. Unacceptable Conditions: No tumor in tissue. Specimens fixed/processed in alternative fixatives (alcohol, Prefer ) or heavy metal fixatives (B-4 or B-5). Frozen specimens. Decalcified specimens. Stability: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable CPT Code(s): Microdissection; Lysis; x2 Amplification; Digestion; Separation and identification; x2 Nucleic 215

6 acid probes; Interpretation and report - Additional CPT code modifiers may be required for procedures performed to test oncologic or inherited disorders EGFR Mutation Detection with Reflex to EML4/ALK Translocation by RT-PCR EGFR RFLX Methodology: Qualitative Polymerase Chain Reaction/Qualitative Reverse Transcription Polymerase Chain Reaction Specimen Required: Collect: Tumor tissue. Specimen Preparation: Formalin fix (10% neutral buffered formalin) and paraffin embed tissue. Transport tissue block or 5 unstained 5 micron slides (Min: 3 slides). AND 6 tissue scrolls (tissue shavings) 10 microns thick. Protect from excessive heat.. Storage/Transport Temperature: Room temperature or refrigerated. Ship in cooled container during summer months. Remarks: Include surgical pathology report. Tissue block will be returned after testing. Unacceptable Conditions: No tumor in tissue. Specimens fixed/processed in alternative fixatives (alcohol, Prefer ) or heavy metal fixatives (B-4 or B-5). Frozen specimens. Decalcified specimens. Stability: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable CPT Code(s): Microdissection; Lysis; x2 Amplification; Digestion; Separation and identification; x2 Nucleic acid probes; Interpretation and report. If reflexed add Lysis; Isolation; Reverse Transcription; x3 Amplification; x3 Gel Separation; Interpretation and report - Additional CPT code modifiers may be required for procedures performed to test oncologic or inherited disorders Ehlers-Danlos Syndrome Kyphoscoliotic Form, Type VI (PLOD1) Deletion/Duplication EDS-VI DD Methodology: Polymerase Chain Reaction/Multiplex Ligation-dependent Probe Amplification Specimen Required: Collect: Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B). Specimen Preparation: Transport 3 ml whole blood. (Min: 1 ml) CPT Code(s): Isolation; x18 Nucleic acid probes; x18 Amplification; x18 Extension; Capillary electrophoresis; Interpretation and report - Additional CPT code modifiers may be required for procedures performed to test for oncologic or inherited disorders Ehlers-Danlos Syndrome Kyphoscoliotic Form, Type VI (PLOD1) Sequencing and EDS-VI FGA Deletion/Duplication Methodology: Polymerase Chain Reaction/Sequencing Specimen Required: Collect: Lavender (EDTA), pink (K 2 EDTA) or yellow (ACD Solution A or B). Specimen Preparation: Transport 3 ml whole blood. (Min: 2 ml) CPT Code(s): Sequencing: Isolation; x19 Amplification; x19 Sequencing; Capillary electrophoresis; Interpretation and report. Del/Dup: x18 Nucleic acid probes; x18 Amplification; x18 Extension; Capillary electrophoresis - Additional CPT code modifiers may be required for procedures performed to test for oncologic or inherited disorders Ehlers-Danlos Syndrome Type VI Screen EDS6 Methodology: High Performance Liquid Chromatography Specimen Required: Patient Preparation: First-morning urine is preferred. Collect: Urine. First-morning void. Also acceptable: Random specimens. Specimen Preparation: Transfer 4 ml urine to an ARUP Standard Transport Tube. (Min: 3 ml) Stability: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 6 months CPT Code(s): Ehrlichia and Anaplasma Species by Real-Time PCR EHRLI ANA Methodology: Real-Time Polymerase Chain Reaction /DNA Probe Hybridization Specimen Required: Collect: Lavender (EDTA). Specimen Preparation: Transfer 5 ml whole blood to ARUP Standard Transport Tubes. (Min: 0.5 ml) Unacceptable Conditions: Specimens collected in green (sodium or lithium heparin) or yellow (ACD). Stability: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Unacceptable CPT Code(s): Ehrlichia chaffeensis Antibodies, IgG & IgM by IFA E CHAF ABS Methodology: Semi-Quantitative Indirect Fluorescent Antibody Transport Tube. (Min: 0.05 ml) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent. Unacceptable Conditions: Contaminated, hemolyzed, or severely lipemic specimens. CPT Code(s): Ehrlichia chaffeensis IgG; Ehrlichia chaffeensis IgM Ehrlichia chaffeensis Antibody, IgG by IFA E CH G Methodology: Semi-Quantitative Indirect Fluorescent Antibody Transport Tube. (Min: 0.05 ml) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent. Unacceptable Conditions: Contaminated, hemolyzed, or severely lipemic specimens. CPT Code(s):

7 Ehrlichia chaffeensis Antibody, IgM by IFA E CH M Methodology: Semi-Quantitative Indirect Fluorescent Antibody Transport Tube. (Min: 0.05 ml) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent. Unacceptable Conditions: Contaminated, hemolyzed, or severely lipemic specimens. CPT Code(s): Ehrlichia chaffeensis DNA, Real-Time PCR EHRLICHIA Methodology: Real-Time Polymerase Chain Reaction Specimen Required: Collect: Lavender (EDTA) or yellow (ACD). Specimen Preparation: Transfer 0.7 ml whole blood or tick (in ethanol or live) to an ARUP Standard Transport Tube. (Min: 0.3 ml or 1 tick) Unacceptable Conditions: Frozen whole blood. Stability: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 1 month (frozen whole blood unacceptable) CPT Code(s): Electrolyte & Osmolality Profile, Fecal FEC PRO Methodology: Quantitative Ion-Selective Electrode/Freezing Point Depression Specimen Required: Collect: 24-hour or random liquid stool. Specimen Preparation: Mix specimen well and transfer 10 g liquid stool to an unpreserved stool transport vial. (ARUP Supply #40910) (Min: 5 g) Do not add saline or water to liquefy specimen. Indicate time and volume. Unacceptable Conditions: Formed or viscous stools. Stability: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 1 month CPT Code(s): Potassium; Sodium; Magnesium; Osmolality Electrolyte Panel LYTES Methodology: Quantitative Ion-Selective Electrode/Enzymatic Specimen Required: Collect: Plasma separator tube or serum separator tube. Specimen Preparation: Allow serum tube to clot completely at room temperature. Separate serum or plasma from cells within 2 hours of collection. Transfer 1 ml serum or plasma to an ARUP Standard Transport Tube. (Min: 0.3 ml) Stability: After separation from cells: Ambient: 4 hours; Refrigerated: 1 week (tightly capped); Frozen: 1 month CPT Code(s): Electrolytes, Fecal FEC LYTES Methodology: Quantitative Ion-Selective Electrode Specimen Required: Collect: 24-hour or random liquid stool. Specimen Preparation: Mix well and transfer a 5 g aliquot of stool to an unpreserved stool transport vial (ARUP Supply #40910). Available online through esupply using ARUP Connect or contact Client Services at (800) (Min: 1 g) Do not add saline or water to liquefy specimen. Unacceptable Conditions: Formed or viscous stool. Stability: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 1 month CPT Code(s): Potassium; Sodium; Chloride Electrolytes, Urine ULYTE Methodology: Quantitative Ion-Selective Electrode Specimen Required: Collect: 24-hour or random urine. Refrigerate during collection. Specimen Preparation: Do not adjust specimen ph. Transfer a 1 ml aliquot of urine from a well-mixed collection to an ARUP Standard Transport Tube. (Min: 0.5 ml) Record total volume and collection time interval on transport tube and test request form. Stability: Ambient: 8 hours; Refrigerated: 1 week; Frozen: 3 months CPT Code(s): Chloride, urine; Potassium, urine; Sodium, urine Elution & Antibody Identification, RBC IRL-ELU Methodology: Hemagglutination Specimen Required: Collect: Lavender (EDTA) or pink (K 2 EDTA). Specimen Preparation: Do not freeze red cells. Transport 5 ml whole blood. Pediatric: Transport 2 ml whole blood. Remarks: Unacceptable Conditions: Separator or gel tubes. CPT Code(s): Elution; AB ID EML4/ALK Translocation by RT-PCR EML4-ALK Methodology: Reverse Transcription Polymerase Chain Reaction Specimen Required: Collect: Tumor tissue. Specimen Preparation: Formalin fix (10% neutral buffered formalin) and paraffin embed tissue. Transport tissue block or 6 tissue scrolls (tissue shavings), 10 microns thick AND an H&E slide (to be retained at ARUP). Protect paraffin block from excessive heat.. 217

8 CPT Code(s): Storage/Transport Temperature: Room temperature or refrigerated. Ship in cooled container during summer months. Remarks: Include surgical pathology report. Unacceptable Conditions: No tumor in tissue. Specimens fixed/processed in alternative fixatives (alcohol, Prefer ) or heavy metal fixatives. Slides. Frozen specimens. Stability: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable Lysis; Isolation; Reverse transcription; x3 Amplification; x3 Gel Separation; Interpretation and report - Additional CPT code modifiers may be required for procedures performed to test for oncologic or inherited disorders Encephalitis Panel, CSF with Reflex to HSV Type 1 & 2 Glycoprotein G-Specific Ab, IgG ENCEPHCSFR Methodology: Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody Specimen Required: Collect: CSF. Specimen Preparation: Transfer 3 ml CSF to an ARUP Standard Transport Tube. (Min: 2 ml) Unacceptable Conditions: Serum or plasma. Contaminated or heat-inactivated specimens. Stability: Ambient: 8 hours; Refrigerated: 2 weeks; Frozen: 1 year CPT Code(s): Measles G; Measles M; Mumps G; Mumps M; VZE; VZM; x2 LCM G/M; HSV MC; HERP I/II; if reflexed, add HERP I; HERP II; Encephalitis Panel, Serum with Reflex to HSV Type 1 and Type 2 Glycoprotein G-Specific Ab, IgG ENCEPH R Methodology: Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody Specimen Preparation: Transfer 3 ml serum to an ARUP Standard Transport Tube. (Min: 1.5 ml) Unacceptable Conditions: Refer to individual components. Stability: After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year CPT Code(s): Measles G; Measles M; Mumps G; Mumps M; VZE; VZM; x2 LCM G/M; HSV MC; HERP I/II; if reflexed, add HERP I; HERP II Endomysial Antibody, IgA by IFA EMAR TITER Methodology: Semi-Quantitative Indirect Fluorescent Antibody Specimen Preparation: Separate serum from cells ASAP. Transfer 1 ml serum to an ARUP Standard Transport Tube. (Min: 0.15 ml) Unacceptable Conditions: Plasma. Severely lipemic, contaminated, or hemolyzed specimens. Stability: After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid freeze/thaw cycles) CPT Code(s): Endomysial Antibody, IgG EMA IGG Methodology: Semi-Quantitative Indirect Fluorescent Antibody Transport Tube. (Min: 0.15 ml) Unacceptable Conditions: Contaminated specimens. CPT Code(s): Entamoeba histolytica (amebiasis), Antibody, IgG AMB Methodology: Semi-Quantitative Enzyme-Linked Immunosorbent Assay Transport Tube. (Min: 0.1 ml) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent. Unacceptable Conditions: Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens. CPT Code(s): Entamoeba histolytica Antigen, EIA AMOEBA Methodology: Qualitative Enzyme Immunoassay Specimen Required: Collect: Random stool. Specimen Preparation: Transfer 5 g stool to an unpreserved stool transport vial (ARUP Supply #40910). Available online through esupply using ARUP Connect or contact ARUP Client Services at (800) Submit specimen according to Biological Substance, Category B, shipping guidelines. Unacceptable Conditions: Specimens in media or preservatives. Stability: Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: 1 week CPT Code(s): Enterovirus Antibody Panel ENT PANEL Methodology: Serum Neutralization Assay/Complement Fixation Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 ml serum to an ARUP Standard Transport Tube. (Min: 0.75 ml) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent." Unacceptable Conditions: Contaminated, hemolyzed, or severely lipemic specimens. CPT Code(s): 86658, x15: Cox (A9), Coxsackie (B1, B2, B3, B4, B5, and B6), Echovirus (Types 6, 7, 9, 11, and 30), Poliovirus (1, 2, and 3) 218

9 Enterovirus Detection by RT-PCR EV PCR Methodology: Qualitative Reverse Transcription Polymerase Chain Reaction Specimen Required: Collect: Lavender (EDTA) or pink (K 2 EDTA). OR CSF. Specimen Preparation: Separate plasma from cells. Transfer 1 ml plasma to a sterile container. (Min: 0.5 ml) Submit specimen according to Biological Substance, Category B, shipping guidelines. Remarks: Specimen source required. Unacceptable Conditions: Heparinized specimens. Non-frozen specimens or specimens exposed to repeated freeze/thaw cycles. Hemolyzed specimens. Stability: Ambient: 1 hour; Refrigerated: 24 hours; Frozen: 3 months CPT Code(s): Enterovirus Typing V ENTTYP Methodology: Immunofluorescence Specimen Required: Patient Preparation: Typing may not be requested directly on a patient specimen. Collect: Enterovirus isolate from cell culture. Specimen Preparation: Transport positive cell culture. Place each specimen in an individually sealed bag. Storage/Transport Temperature: Room temperature. Submit specimen according to Biological Substance, Category B, shipping guidelines. Remarks: Specimen source preferred. Stability: Ambient: 72 hours; Refrigerated: 72 hours; Frozen: 1 month CPT Code(s): Tissue culture; Additional studies Eosinophil Cationic Protein ECP Methodology: Quantitative Fluorescent Enzyme Immunoassay (FEIA) Specimen Preparation: Collect a FULL serum separator tube of blood. Invert 5 times. Let clot 60 to 120 minutes at room temperature. Centrifuge at 1000 to 1300 g for 10 minutes. Transfer 1 ml serum to an ARUP Standard Transport Tube and freeze immediately. (Min: 0.5 ml) Unacceptable Conditions: Thawed specimens. Plasma and hemolyzed serum. Stability: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 1 month CPT Code(s): Eosinophil Granule Major Basic Protein in Tissues EMBP TISS Methodology: Immunofluorescence/Affinity Chromatography Specimen Required: Collect: Tissue. Specimen Preparation: Formalin fix or formalin fix and paraffin embed. Transport 5 micron tissue sections on three slides (no specific size requirement available). Unacceptable Conditions: Frozen fixed tissue. Thawed tissue. Stability: Ambient: In formalin: 3 weeks, Paraffin block: Indefinitely; Frozen (no medium): Indefinitely CPT Code(s): Immunocytochemistry; Consultation & report Eosinophilia Panel by FISH FISH EOS P Methodology: Fluorescence in situ Hybridization Specimen Required: Collect: Non-diluted bone marrow collected in a heparinized syringe. Also acceptable: Green (sodium heparin). Other specimen types may be acceptable, contact the Cytogenetics Laboratory for specific specimen collection and transportation instructions. Specimen Preparation: Transfer 3 ml bone marrow to a green (sodium heparin) (Min: 1 ml). OR transport 10 ml whole blood (Min: 2 ml) Storage/Transport Temperature: Room temperature. Remarks: It is recommended that all FISH studies be done in conjunction with routine cytogenetic analysis. (Refer to appropriate Chromosome Analysis test for order instructions). If FISH only is requested, please include copy of previous cytogenetics report. If cell pellets or dropped cytogenetics slides are not submitted, a processing fee will apply. This test must be ordered using Cytogenetic test request form #43099 or through your ARUP interface. Submit the Patient History for Cytogenetics (Chromosome) Studies form with the electronic packing list (available at Unacceptable Conditions: Frozen specimens. Paraffin-embedded specimens. Clotted specimens. Stability: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable CPT Code(s): x4 DNA probe each; x4 Interphase in situ hybridization each; Interpretation and report - Additional CPT codes modifiers may be required for procedures performed to test for oncologic or inherited disorders Eosinophils, Nasal EOS NASAL Methodology: Wright's Stain/Microscopy Specimen Required: Collect: Nasopharyngeal swab. Specimen Preparation: Transport nasopharyngeal swab or prepared slides. Storage/Transport Temperature: Room temperature. Transport in a protective container. Unacceptable Conditions: Frozen specimens. Stability: Swab: Ambient: 24 hours; Refrigerated: 24 hours; Frozen: Unacceptable Prepared Unstained Slides: Ambient: 1 week; Refrigerated: 1 week; Frozen: Unacceptable CPT Code(s): Eosinophils, Sputum EOS SPUTUM Methodology: Wright's Stain/Microscopy Specimen Required: Collect: Sputum. Specimen Preparation: Transport 0.5 ml sputum or prepared slides. Storage/Transport Temperature: Room temperature. Transport in a protective container. Unacceptable Conditions: Frozen specimens. 219

10 Stability: Sputum: Ambient: 8 hours; Refrigerated: 24 hours; Frozen: Unacceptable Unstained Slides: Ambient: 1 week; Refrigerated: 1 week; Frozen: Unacceptable CPT Code(s): Ephedrine, Serum or Plasma EPHEDRIN Methodology: High Performance Liquid Chromatography/Mass Spectrometry Specimen Required: Collect: Plain red, gray (sodium fluoride/potassium oxalate), green (sodium heparin), lavender (EDTA), or pink (K 2 EDTA). Specimen Preparation: Transfer 1 ml serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 ml) Unacceptable Conditions: Serum separator tubes. Stability: Ambient: 2 weeks; Refrigerated: 2 weeks; Frozen: 2 weeks CPT Code(s): Ephedrine, Urine EPHEDRI U Methodology: High Performance Liquid Chromatography/Mass Spectrometry Specimen Required: Collect: Random urine. Specimen Preparation: Transfer 1 ml urine to an ARUP Standard Transport Tube. (Min: 0.5 ml) Stability: Ambient: 2 weeks; Refrigerated: 2 weeks; Frozen: 2 weeks CPT Code(s): Epithelial Basement Membrane Zone IgA Antibodies EBMZ IGA Methodology: Indirect Immunofluorescence Specimen Required: Collect: Plain red or serum separator tube. Specimen Preparation: Transfer 2 ml serum to an ARUP Standard Transport Tube. (Min: 0.5 ml) Remarks: For specimens less than 0.5 ml, call the Immunodermatology lab at (866) Unacceptable Conditions: Hemolyzed or lipemic specimens. Stability: Ambient: 4 days; Refrigerated: 3 months; Frozen: Indefinitely CPT Code(s): x2 Substrates (monkey esophagus and human split skin) Epithelial Basement Membrane Zone IgG Antibodies EBMZ IGG Methodology: Indirect Immunofluorescence (IFA) Specimen Required: Collect: Plain red or serum separator tube. Specimen Preparation: Transfer 2 ml serum to an ARUP Standard Transport Tube. (Min: 0.5 ml) Remarks: For specimens less than 0.5 ml, call the Immunodermatology lab at (866) Unacceptable Conditions: Hemolyzed or lipemic specimens. Stability: Ambient: 4 days; Refrigerated: 3 months; Frozen: Indefinitely CPT Code(s): x2 Substrates (monkey esophagus and human split skin) Epithelial Membrane Antigen (EMA) by Immunohistochemistry EMA IHC Methodology: Immunohistochemistry Specimen Required: Collect: Tissue or cells. Specimen Preparation: Formalin fix (10% neutral buffered formalin is preferred) and paraffin embed specimen (Cells must be prepared into a cellblock). If sending precut slides, do not oven bake. Protect paraffin block and/or slides from excessive heat. Ship in cooled container during summer months. Storage/Transport Temperature: Room temperature or refrigerated. Remarks: IMMUNOHISTOCHEMISTRY ORDERING AND SUBMISSION DETAILS: Submit electronic request. If you do not have electronic ordering capability, use an ARUP Immunohistochemistry Stain Form (#32978) with an ARUP client number. For additional technical details, please contact ARUP Client Services. Unacceptable Conditions: Depleted specimens. Specimens submitted with non-representative tissue type. Stability: Ambient: Indefinite; Refrigerated: Indefinite; Frozen: Indefinite CPT Code(s): Epithelial-Related Antigen, MOC-31 by Immunohistochemistry ERA IHC Methodology: Immunohistochemistry Specimen Required: Collect: Tissue or cells. Specimen Preparation: Formalin fix (10% neutral buffered formalin is preferred) and paraffin embed specimen (Cells must be prepared into a cellblock). If sending precut slides, do not oven bake. Protect paraffin block and/or slides from excessive heat. Ship in cooled container during summer months. Storage/Transport Temperature: Room temperature or refrigerated. Remarks: IMMUNOHISTOCHEMISTRY ORDERING AND SUBMISSION DETAILS: Submit electronic request. If you do not have electronic ordering capability, use an ARUP Immunohistochemistry Stain Form (#32978) with an ARUP client number. For additional technical details, please contact ARUP Client Services. Unacceptable Conditions: Depleted specimens. Specimens submitted with non-representative tissue type. Stability: Ambient: Indefinite; Refrigerated: Indefinite; Frozen: Indefinite CPT Code(s): Epithelial Skin Antibody EPITHELIAL Methodology: Indirect Immunofluorescence Specimen Required: Collect: Plain red or serum separator tube. Specimen Preparation: Transfer 2 ml serum to an ARUP Standard Transport Tube. (Min: 0.5 ml) Storage/Transport Temperature: Room temperature. Remarks: For specimens less than 0.5 ml, call the Immunodermatology lab at (866) Unacceptable Conditions: Hemolyzed or lipemic specimens. Stability: Ambient: 4 days; Refrigerated: 3 months; Frozen: Indefinitely CPT Code(s): x4 Substrates (BMZ, cell surface, IgG and IgA) 220

11 Epstein-Barr Virus Antibody Panel I EBV PAN Methodology: Semi-Quantitative Chemiluminescent Immunoassay Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transport 2 ml serum. (Min: 0.5 ml) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of acute specimens. Mark specimens plainly as "acute" or "convalescent." Unacceptable Conditions: Urine. Contaminated or heat-inactivated specimens. CPT Code(s): EBV VCA IgG; EBV VCA IgM; EBV NA; EBV Early Epstein-Barr Virus Antibody Panel II EBV PAN 2 Methodology: Semi-Quantitative Chemiluminescent Immunoassay Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 ml serum to an ARUP Standard Transport Tube. (Min: 0.5 ml) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimen plainly as "acute" or "convalescent." Unacceptable Conditions: Urine. Contaminated or heat-inactivated specimens. CPT Code(s): EBV VCA IgG; EBV VCA IgM Epstein-Barr Virus Antibody to Early D Antigen (EA-D), IgG EBV EAD Methodology: Semi-Quantitative Chemiluminescent Immunoassay Specimen Preparation: Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimen plainly as "acute" or "convalescent." Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 ml serum to an ARUP Standard Transport Tube. (Min: 0.5 ml) Unacceptable Conditions: Urine. Contaminated or heat-inactivated specimens. CPT Code(s): Epstein-Barr Virus Antibody to Nuclear Antigen, IgG EBV NA Methodology: Semi-Quantitative Chemiluminescent Immunoassay Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 ml serum to an ARUP Standard Transport Tube. (Min: 0.5 ml) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimen plainly as "acute" or "convalescent." Unacceptable Conditions: Urine. Contaminated or heat-inactivated specimens. CPT Code(s): Epstein-Barr Virus Antibody to Viral Capsid Antigen, IgA EBV A Methodology: Semi-Quantitative Enzyme-Linked Immunosorbent Assay Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 0.5 ml serum to an ARUP Standard Transport Tube. (Min: 0.1 ml) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimen plainly as "acute" or "convalescent." Unacceptable Conditions: Contaminated or heat-inactivated specimens. Stability: After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year CPT Code(s): Epstein-Barr Virus Antibody to Viral Capsid Antigen, IgG EBV G Methodology: Semi-Quantitative Chemiluminescent Immunoassay Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 ml serum to an ARUP Standard Transport Tube. (Min: 0.5 ml) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent." Unacceptable Conditions: Urine. Contaminated or heat-inactivated specimens. CPT Code(s): Epstein-Barr Virus Antibody to Viral Capsid Antigen, IgG & IgA EBV PAN 3 Methodology: Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Chemiluminescent Immunoassay Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 ml serum to an ARUP Standard Transport Tube. (Min: 0.5 ml) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent." Unacceptable Conditions: Urine. Contaminated or heat-inactivated specimens. CPT Code(s): x2 221