injections. Needle Tip 1 µl Cone 1BR

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1 s General Purpose Manual s µl Nano With the ability to inject down to 50 nl with high precision and accuracy, SGE Nano s are perfect for Nano capillary chromatography injection as well as making accurate standards that require small volumes. The sample is only drawn into the needle, not the syringe barrel. When the plunger is depressed, the sample is completely dispensed by the Nano plunger that extends to the tip of the needle. Displacing the full sample during injection results in virtually zero dead volume and carry over between Reduced Diameter injections. The plungers and needles are matched sets and must be replaced as a set. Refer to page 65 for a list of replacement plunger and needle kits. Sample NOZZLE TIP SHOWN IN DIAGRAM. s and ± 2 % (dispensed volume) Borosilicate Glass Barrel Outer Diameter () 6.5 mm and 8 mm Scale 0.5 µl (6.5 mm barrel) = mm, 0.5 µl (8 mm barrel) = 63.7 mm, 1 µl = 54.1 mm, 63.7 mm, 5 µl = 48.7 mm Code and 6.5 mm Outer Diameter () Barrel 0.5 µl Cone 0.5BNR µl Bevel 0.5BNR-5BV µl Cone 0.5BNR-5/ mm Outer Diameter () Barrel 0.5 µl Cone 0.5BR µl Bevel 0.5BR-7BV µl Cone 1BR µl Bevel 1BR-5BV µl Cone 1BR µl Bevel 1BR-7BV µl Cone 1BR µl Cone 1BR-7/ µl Cone 5BR µl Bevel 5BR-5BV µl Cone 5BR µl Bevel 5BR-7BV µl Cone 5BR µl Nozzle 5BR-7N When replacing the plunger and needle follow the instructions included in the kit the front cover nut must be loosened before removing the plunger. s Fitted with Repeating Adaptor See page 63 for more details about the RAX repeating adaptor. Code and 6.5 mm Outer Diameter () Barrel 0.5 µl Cone 0.5BNR-5-RAX µl Cone 0.5BNR-5/0.47-RAX mm Outer Diameter () Barrel 0.5 µl Cone 0.5BR-7-RAX µl Cone 1BR-5-RAX µl Cone 1BR-7-RAX s General Purpose Manual s 35

2 s General Purpose Manual s 5-10 µl Metal s and Borosilicate Glass Barrel Outer Diameter () Scale ± 1 % (dispensed volume) 6.5 mm (guided plunger syringes 8 mm) 54.1 mm Standard Protection s Fixed Code 10 Pack 25 Pack 5 µl Bevel 5F µl Bevel 5F µl Cone 5F /0.63C 10 µl Bevel 10F µl Bevel 10F µl cone 10F-5C µl Bevel 10F µl Cone 10F- 4.2/0.63C Removable µl Bevel 5R µl Bevel 10R µl Bevel 10R SuperFlex s protection helps prevent plunger bending during injection. Code No Fixed 5 µl Bevel 5FX µl Cone 5FX-5C µl Bevel 10FX µl Bevel 10FX µl Cone 10FX-5C µl Cone 10FX-5/0.63C Removable 5 µl Bevel 5RX µl Bevel 10RX The virtually indestructible Titanium Nickel alloy plunger of the SuperFlex s is ideal for student use. 36 This selection guide can be viewed as an electronic book at sge.com/selectionguide

3 s and Guided s Guided plunger syringes are the most rugged syringe available, making them perfect for industrial environments. Fixed Code No 5 µl Bevel 5F-GP µl Bevel 10F-GP Removable 5 µl Bevel 5R-GP µl Bevel 5R-GP µl Bevel 5R-GPS * 10 µl Bevel 10R-GP µl Bevel 10R-GP * Short half scale syringe (scale length 27 mm). s Fitted with Repeating Adaptor See page 63 for more details about the RAX repeating adaptor. Fixed Code No 10 µl Bevel 10F-RAX Removable 5 µl Bevel 5R-RAX µl Bevel 10R-RAX Removable - Guided 5 µl Bevel 5R-GP-RAX µl Bevel 10R-GP-RAX µl Bevel 10R-GP-7-RAX The RAX repeating adaptor improves precision and reproducibility when repeatedly injecting the same volume manually. s General Purpose Manual s 37

4 s General Purpose Manual s µl Metal s and Borosilicate Glass Barrel Outer Diameter () Scale ± 1 % (dispensed volume) 8 mm 60 mm Code Fixed 25 µl Bevel 25F µl Bevel 50F µl Bevel 100F µl Bevel 250F µl Bevel 500F Removable 25 µl Bevel 25R µl Bevel 50R µl Bevel 100R µl Bevel 100R µl Bevel 250R µl Bevel 500R s Fitted with Repeating Adaptor See page 63 for more details about the RAX repeating adaptor. Code Removable 25 µl Bevel 25R-RAX µl Bevel 50R-RAX µl Bevel 100R-RAX µl Bevel 250R-RAX µl Bevel 500R-RAX To eliminate carryover between samples, flush the syringe with solvent 5-20 times, remembering to discard the first 2-3 washes. 38 For more syringe tips view our video at sge.com/support/videos

10-500 µl PTFE ped Borosilicate Glass Barrel Outer Diameter () Scale ± 1 % (dispensed volume) 10 µl = 6.5 mm, 25 500 µl = 8 mm 10 µl = 54.

20 Bevel 25F-GT - 031815-003200 50 µl 50 25 0.50 0.20 Bevel 50F-GT - 031820-004200 100 µl 50 25 0.50 0.20 Bevel 100F-GT - 031825-005200 250 µl 50 25 0.50 0.20 Bevel 250F-GT - 031830-006200 500 µl 50 25 0.

50 0.20 Bevel 100R-GT 038110 031825-005250 250 µl 50 25 0.50 0.20 Bevel 250R-GT 038110 031830-006250 500 µl 50 25 0.50 0.20 Bevel 500R-GT 038110 031835-007250 Guided 10 µl 50 26 0.47 0.

5 µl PTFE ped Borosilicate Glass Barrel Outer Diameter () Scale ± 1 % (dispensed volume) 10 µl = 6.5 mm, µl = 8 mm 10 µl = 54.1 mm, µl = 60 mm s and Fixed PTFE ped Code 10 µl Bevel 10F-GT µl Bevel 10F-GT µl Bevel 25F-GT µl Bevel 50F-GT µl Bevel 100F-GT µl Bevel 250F-GT µl Bevel 500F-GT Removable 10 µl Bevel 10R-GT µl Bevel 25R-GT µl Bevel 50R-GT µl Bevel 100R-GT µl Bevel 250R-GT µl Bevel 500R-GT Guided 10 µl Bevel 10R-GP-GT On-Column 10 µl On- Column Fixed Luer 10R-GT-OC- CE µl F-LT-GT µl F-LT-GT µl F-LT-GT µl F-LT-GT Fixed Luer Lock 50 µl F-LL-GT µl F-LL-GT µl F-LL-GT µl F-LL-GT s General Purpose Manual s 39

s General Purpose Manual s 1-100 ml PTFE ped Also Suitable for Pumps and Dispensers s and Borosilicate Glass Barrel Outer Diameter () Scale Thread in Button ± 1 % (dispensed volume) 1 ml = 9 mm, 2.

6 s General Purpose Manual s ml PTFE ped Also Suitable for Pumps and Dispensers s and Borosilicate Glass Barrel Outer Diameter () Scale Thread in Button ± 1 % (dispensed volume) 1 ml = 9 mm, 2.5 ml = 11 mm, 5 ml = 14 mm, 10 ml = 18 mm, 25 ml = 27 mm, 50 ml = 32.8 mm, 100 ml = 40.8 mm 1 25 ml = 60 mm, 50 ml = 84.2 mm, 100 ml = 104 mm 6-32 UNC PTFE ped Code Fixed 1 ml Bevel 1MDF-GT ml Bevel 2.5MDF-GT Removable 1 ml Bevel 1MR-GT * 2.5 ml Bevel 2.5MDR-GT ml Bevel 5MDR-GT ml Bevel 10MDR-GT Fixed Luer 1.0 ml Luer 1MF-LT-GT * 2.5 ml Luer 2.5MDF-LT-GT Fixed Luer Lock 1.0 ml Luer Lock 1MDF-LL-GT ml Luer Lock 2.5MDF-LL-GT ml Luer Lock 5MDF-LL-GT ml Luer Lock 10MDF-LL-GT ml Luer Lock 25MDF-LL-GT Removable Luer Lock 5 ml Luer Lock 5MDR-LL-GT ml Luer Lock 10MDR-LL-GT ml Luer Lock 25MR-LL-GT ml Luer Lock 50MR-LL-GT * 100 ml Luer Lock 100MR-LL-GT * * No thread in plunger stem of syringe part numbers , , and s Valves valves are ideal for sample storage and transportation or to pressurize sample prior to injection. SGE offers three basic types of manually operated syringe valves; push-pull for smaller volumes (25 µl 2.5 ml), septum and pushbutton for larger volumes (5 ml 2 L). SGE Luer, Luer Lock and removable needle syringes can be fitted with valves. The valve allows fluid to be stored in the syringe without contamination. 40 For more syringe tips view our video at sge.com/support/videos

Push-pull Valves Septum Valve Push-button Valves CLOSE OPEN OPEN CLOSE (Turn to close) OPEN s and 031905 and 031906 031907 Push-pull valves suit removable needle 25 µl - 2.5 ml syringes.

7 Push-pull Valves Septum Valve Push-button Valves CLOSE OPEN OPEN CLOSE (Turn to close) OPEN s and and Push-pull valves suit removable needle 25 µl ml syringes The septum cap (P/N ) allows sample access without loss or contamination. It can be removed and Luer Lock needles or devices fitted Suits 5 ml - 2 L syringes with removable Luer Lock or removable needle Suitable for any Luer Lock and Luer syringes or other Luer devices. With the valve in the closed position it is possible to move the plunger to pre-pressurize the sample. Description Valve Code Valve Push-pull Valve with Replaceable µl ml V25/2.5M-0.63C * Push-pull Valve with Replaceable µl ml V25/2.5M-0.47C ˆ Push-pull Valve with Luer Lock 25 µl ml VLL25/2.5M Push-button Valve with Luer Lock or Removable 5 ml - 2 L VLLMA Septum Valve 5 ml - 2 L VLLMA5/ Push-button Valve for Luer Lock Devices 50 µl - 2 L SLLV * 50 mm, 0.63 mm cone tip needle. ˆ 50 mm, 0.47 mm cone tip needle. s Pre-fitted with Valves PTFE ped Code Valve Pre-fitted with Removable Push-pull Valve 50 µl 50R-V-GT µl 100R-V-GT µl 250R-V-GT µl 500R-V-GT ml 1MR-V-GT ml 2.5MDR-V-GT Pre-fitted with Luer Lock Valve 1 ml 1MR-VLL-GT ml 2.5MDR-VLL-GT ml 5MDR-VLLMA-GT ml 10MDR-VLLMA-GT ml 25MDR-VLLMA-GT ml 50MR-VLLMA-GT ml 100MR-VLLMA-GT Valve s For use with P/N and Pack Size Valve Cone Side Hole/Dome Cone See page 67 for the complete range of Luer Lock needles suitable for use on Luer Lock valves. s Valves 41

The SealTight Valves are completely inert and enable connection of a variety of fittings.

8 s valves SealTight s and SealTight low pressure valves are designed for improved valve seal life. SGE valves have overcome the problem of leakage by reducing perpendicular rotor force, and optimizing the rotor face union, thereby improving PTFE plug integrity. The end result is a longer life seal. The SealTight Valves are completely inert and enable connection of a variety of fittings. Valves are available in standard (SV) or bulkhead panel mounting connection (SVP) which can be easily mounted to a dispenser. Other valves, including custom OEM configurations, are available. Flow Path 1.5 mm (0.059") Threaded Ports 1/4 28 UNF Pressure Rating 120 psi Temperature Rating C Standard Weight 58 g Bulkhead Weight 65 g Breakaway Torque 0.15 Nm Lifetime Testing 8,000 cycles ADVANTAGES Extended life resulting from face rotor seal technology, making valves cost effective and reliable. Inert PTFE and Kel-F flow path compatible with a variety of solvents and sample matrices. Easily reassembled by hand: no need to torque it up like other valves. Screw thread for connection onto 5 ml 2 L syringes using syringe-valve connector ( ). APPLICATIONS Description Mounting Code Distribution Valves 2-way L Bulkhead SVP-2L/D way T Standard SV-3T/D way T Bulkhead SVP-3T/D Port Valves I flow 2 port Standard SV-2I/I T flow 3 port Standard SV-3T/T T flow 3 port Bulkhead SVP-3T/T L flow 3 port Standard SV-3T/L L flow 3 port Bulkhead SVP-3T/L SealTight Valve Fittings and Connectors 2-way L 3-way T I flow 2 port T flow 3 port L flow 3 port SealTight valves offer the versatility to be utilized in a variety of industries and applications: Dispensers. Diluters. Flow path variation. Biocompatible, suitable for use in pathology equipment. Description Code Kel-F Female Luer KSV-FL Kel-F Male Luer KSV-LT Metal Thread Male Luer Lock - PTFE Flow Path MSV-LL Valve Connector (fits 5 ml 2 L syringes) PTFE Flow Path MSV-SYR Flangeless Tubing Connector for 1/16" Tubing (includes 2 ferrules) MSV-1/ Flangeless Tubing Connector for 1/8" Tubing (includes 2 ferrules) MSV-1/8FLT Ferrules for 1/16" Tubing PF Ferrules for 1/8" Tubing PF s Valves

Needle Gauge. Needle OD (mm) Needle ID (mm)

Needle Gauge. Needle OD (mm) Needle ID (mm) s LC Manual s Beckman/Altex, Rheodyne, SSI Instruments and Valco Valves s and Borosilicate Glass Barrel Outer Diameter () Scale 5 µl and 10 µl = 6.5 mm, 25 µl to 500 µl = 8 mm, 1 ml = 8.8 mm, 2.5 ml =