InfinityLab Purification Capillary Kits
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1 InfinityLab Purification Capillary Kits Technical Note This note describes the capillary connections for Agilent preparative systems. Agilent Technologies
2 Contents Abbreviations 3 List of Modules 4 Overview 5 General Installation Notes 5 Installation Notes for 1260 Infinity II Column Organizer G9328A 6 Installation Notes for 1290 Infinity II Column Compartment G7163B 8 Configurations with 1260 Infinity II Column Organizer (G9328A) 10 Entry Level System Configurations (with Manual Injector) 10 Standard UV/LC System Configurations 16 Standard Mass-Based Systems Configuration 24 Configurations with 1290 Infinity II Preparative Column Compartment (G7163B) 28 Entry Level System Configurations (with Manual Injector) 28 Standard UV/LC System Configurations 34 Standard Mass-Based System Configurations 40 Accessories 44 UV Accessories 44 MS Accessories 44 2
3 Abbreviations Table 1 Abbreviations Modules VWD Variable wavelength detector Diode array detector MI Manual Injector DC Delay Coil Organizer RC Recovery collector FC Fraction collector PRV Pressure relieve valve BPR Back pressure regulator FM Flow modulator Fittings R fitting for flex capillaries: for rigid capillaries: S lock fitting Capillaries and tubings UV1...5 Capillaries to connect the s of the liquid chromatographic part of the system MS1...6 Capillaries to connect the s of the mass spectrometric part of the system 0.3f 0.3 ID flex capillary 0.5r 0.5 ID rigid capillary ESD Electrostatic discharge Material SST Stainless steel PTFE Polytetrafluorethylene PEEK Polyether ether ketone 3
4 List of Modules Table 2 G7161A G7161B G7114A G7115A G7165A G7159B G7166A G1364E G1328D G7157A G7170B G9324A List of s 1260 Infinity II Preparative Binary Pump 1290 Infinity II Preparative Binary Pump 1260 Infinity II Variable Wavelength Detector 1260 Infinity II Diode Array Detector WR 1260 Infinity II Multiple Wavelength Detector 1290 Infinity II Preparative Open-Bed Fraction Collecor 1260 Infinity II Preparative Valve-Based Fraction Collector 1260 Infinity II Preparative Fraction Collector 1260 Infinity II Manual Preparative Injector 1260 Infinity II Preparative Autosampler 1290 Infinity II MS Flow Modulator 1260 Infinity II Delay Coil Organizer PREP Pressure Relief Valve Kit G7163B G9328A G7110A 1290 Infinity II Preparative Column Compartment 1260 Infinity II Column Organizer 1260 Infinity II Isocratic Pump ERI Remote and Leak Module G6125B MSD 4
5 Overview General Installation Notes Capillaries and Fittings Capillary UV/LC kits ( , , , ) are flow dependent. The proper kit needs to be selected based on the desired flow range. The MS Upgrade kit ( ) is only one kit. It contains all capillaries for all flow ranges. Four capillaries (UV2A, 2B, 2C, 2D) are provided to connect sampler and column. All capillaries with an inner diameter (ID) of 0.3 and 0.5 are flexible. Only the MS1B capillary from column to flow modulator is always rigid. This capillary is guided between s and needs to remain in its fixed position. The bypass of the flow modulator is part of accessory kit G All capillaries going to the ports of the flow modulator need UHP-FF fittings. NOTE Do not use swagelock for the flow modulator, the ports can be damaged. The Multiple Wavelength Detector is not depicted in the drawings. The same principles as for the Diode Array Detector apply. Only removable fittings are used for columns. Either Quick Turn Fittings for flexible capillaries, or UHP-FF for rigid capillaries. General rules If the height of the stack configuration exceeds the safety limit (0.89 m), the solvent cabinet has to be placed next to the stack on the base plate. The base plate is needed in case the Solvent Cabinet is placed on a bench by itself, and has to be ordered separately (p/n G ). 5
6 Installation Notes for 1260 Infinity II Column Organizer G9328A Columns and Column Holders To keep the capillary from column to detector as short as possible, the column needs to be mounted at the highest possible position, and in the front of the column organizer. Two capillaries (UV2A, UV2B) are provided to connect sampler and column: UV2A is used for columns shorter than 15 cm, UV2B is used for long columns of 15 cm and more. Fix columns with the following column holders: M size holders ( ) for 9.4 /10, 21.2 and 30 columns, L size holders (G ) for 50 columns, L size holders have to be ordered separately. The 25 cm columns require two M size holders, shorter columns need only one holder. One M holder can be used for pre-column. Manual Injection Valve G1328D (MI) The MI must be installed in the first groove of the column organizer extrusion plate. The MI can be installed at any height. In combination with columns shorter than 25 cm, the MI can be positioned in the middle of the column organizer. In combination with columns 25 cm or longer, the MI needs to be installed at the lowest possible position. The MI requires a suitable waste container (bottle or beaker) for waste and siphoning capillaries. NOTE The kit does not contain a waste container. The waste container must be provided by the customer. The waste container has to be placed on the leak plate. 6
7 In the case that a 20 ml stainless steel loop is used, it has to be bent in the direction of the extrusion to leave enough space for the container on the leak plate. None of the other loops limit the space for the container. The waste and siphoning capillaries have to be bent. The end of the capillaries have to be at the same height as the position of the injection needle. Pressure Relieve Valve (PRV, ) and Back Pressure Regulator (BPR, ) PRV must be installed with every configuration. NOTE The PRV is required for safety reasons. The PRV and BPR come with the Preparative LC system. The PRV is installed at the outlet of the Delay Coil Organizer, if available. If the PRV is used as a single item, the following measures are required: The PRV has to be connected with system tubing from the detector. This connection requires union to ¼-28. The PRV has to be fixed to the column organizer using S holders. The PRV has to be placed on the upper part of the extrusion. NOTE The length of the capillary from the detector to the PRV is intentionally limited. The PRV waste tubing is guided to the leak plate by S holders. The BPR needs to be installed ahead of the Recovery Collector. The use of BPR is recoended only for certain flow rates, check for operating instructions. The BPR can be fixed at any position on the extrusion using S holders. 7
8 Installation Notes for 1290 Infinity II Column Compartment G7163B General The Delay Coil Organizer must be installed at the highest valve position. The Flow Modulator must be installed in the second highest valve position. The Recovery Collector can be installed at any available free valve position. Columns and Column Holders The column should be mounted at the highest possible position of the Column Compartment. The capillaries UV2A, UV2B, UV2C and UV2D are used to connect the column with the sampler. Select the suitable capillary length depending on the position or length of the column. Fix columns with the following column holders: M size holders ( ) for 9.4 /10, 21.2 and 30 columns, L size holders (G ) for 50 columns, The 25 cm columns require two M size holders, shorter columns need only one M size holder. One M size holder can be used for pre-column. Manual Injection Valve G1328D (MI) The MI should be installed on the bottom part of the small mounting plate of the Column Compartment in the first groove. The MI requires a suitable waste container (bottle or beaker) for waste and siphoning capillaries. NOTE The kit does not contain a waste container. The waste container must be provided by the customer. The waste container has to be placed on the plateau of the Column Compartment. Columns need to be installed such that they do not collide with MI, loop or siphoning capillaries, usually on the left part of the extrusion. 8
9 The waste and siphoning capillaries have to be bent. The end of the capillaries have to be at the same height as the position of injection needle and should point down to the waste container. Pressure Relieve Valve (PRV, ) and Back Pressure Regulator (BPR, ) PRV must be installed with every configuration. NOTE The PRV is required for safety reasons. The PRV and BPR come with the Preparative LC system. The PRV is installed at the outlet of the Delay Coil Organizer, if available. If the PRV is used as a single item, the following measures are required: The PRV has to be connected with system tubing from the detector. This connection requires union to ¼-28. The PRV has to be fixed to the Column Compartment using S holders ( ). The PRV has to be placed on the upper part of the extrusion. NOTE The length of the capillary from the detector to the PRV is intentionally limited. The PRV waste tubing is guided to the leak sensor by S holders. The use of BPR is recoended only for certain flow rates, check for operating instructions. The BPR can be fixed at any position on the extrusion using S holders. 9
10 Configurations with 1260 Infinity II Column Organizer (G9328A) Entry Level System Configurations (with Manual Injector) Entry Level System Configurations Table 3 Capillary Kit Prep UV/LC Overview System Capillary Kit Prep LC/UV kit for each flow rate Flow range 4 8mL/min ml/min 80 From To Length /cm Fittings UV1 Pump Sampler f UV2A Sampler Column f UV2B Sampler Column f UV3 Column VWD/ f UV4 VWD/ DC Box/ PRV f r UV5 VWD/ PRV f
11 Table 4 Preparative Open-Bed Fraction Collector tubing kits (G7159B) P/N Preparative Open-Bed Fraction Collector tubing kit Tubing Kit 8 (G ) Tubing Kit 50 (G ) Tubing Kit 150 (G ) Tubing Kit 200 (G ) Suitable for the flow up to 1 8 ml/min ml/min ml/min 150 Table 5 Preparative Fraction Collector tubing kits (G1364E) P/N Preparative Fraction Collector tubing kit G G G Coent 4 8 ml/min ml/min 11
12 12
13 Entry Level System Configurations with Delay Coil Organizer (G9324A) Table 6 Capillary Kit Prep UV/LC Overview System Capillary Kit Prep LC/UV kit for each flow rate Flow range 4 8mL/min ml/min 80 From To Length /cm Fittings UV1 Pump Sampler f UV2A Sampler Column f UV2B Sampler Column f UV3 Column VWD/ f UV4 VWD/ DC Box/ PRV f r UV5 VWD/ PRV f Table 7 Preparative Open-Bed Fraction Collector tubing kits (G7159B) P/N Preparative Open-Bed Fraction Collector tubing kit Tubing Kit 8 (G ) Tubing Kit 50 (G ) Tubing Kit 150 (G ) Tubing Kit 200 (G ) Suitable for the flow up to 1 8 ml/min ml/min ml/min
14 Table 8 Preparative Fraction Collector tubing kits (G1364E) P/N Preparative Fraction Collector tubing kit G G G Coent 4 8 ml/min ml/min 14
15 15
16 Standard UV/LC System Configurations Configuration with Preparative Open-Bed Fraction Collector Table 9 Capillary Kit Prep UV/LC Overview System Capillary Kit Prep LC/UV kit for each flow rate Flow range 4 8mL/min ml/min 80 From To Length /cm Fittings UV1 Pump Sampler f UV2A Sampler Column f UV2B Sampler Column f UV3 Column VWD/ f UV4 VWD/ DC Box/ PRV f r UV5 VWD/ PRV f Table 10 Preparative Open-Bed Fraction Collector tubing kits (G7159B) P/N Preparative Open-Bed Fraction Collector tubing kit Tubing Kit 8 (G ) Tubing Kit 50 (G ) Tubing Kit 150 (G ) Tubing Kit 200 (G ) Suitable for the flow up to 1 8 ml/min ml/min ml/min
17 17
18 Configuration with Preparative Open-Bed Fraction Collector and Delay Coil Organizer (G9324) Table 11 Capillary Kit Prep UV/LC Overview System Capillary Kit Prep LC/UV kit for each flow rate Flow range 4 8mL/min ml/min 80 From To Length /cm Fittings UV1 Pump Sampler f UV2A Sampler Column f UV2B Sampler Column f UV3 Column VWD/ f UV4 VWD/ DC Box/ PRV f r UV5 VWD/ PRV f Table 12 Preparative Open-Bed Fraction Collector tubing kits (G7159B) P/N Preparative Open-Bed Fraction Collector tubing kit Tubing Kit 8 (G ) Tubing Kit 50 (G ) Tubing Kit 150 (G ) Tubing Kit 200 (G ) Suitable for the flow up to 1 8 ml/min ml/min ml/min
19 19
20 Configuration with Preparative Fraction Collector Table 13 Capillary Kit Prep UV/LC Overview System Capillary Kit Prep LC/UV kit for each flow rate Flow range 4 8mL/min ml/min 80 From To Length /cm Fittings UV1 Pump Sampler f UV2A Sampler Column f UV2B Sampler Column f UV3 Column VWD/ f UV4 VWD/ DC Box/ PRV f r UV5 VWD/ PRV f Table 14 Preparative Fraction Collector tubing kits (G1364E) P/N Preparative Fraction Collector tubing kit G G G Coent 4 8 ml/min ml/min 20
21 21
22 Configuration with Preparative Fraction Collector and Delay Coil Organizer (G9324) Table 15 Capillary Kit Prep UV/LC Overview System Capillary Kit Prep LC/UV kit for each flow rate Flow range 4 8mL/min ml/min 80 From To Length /cm Fittings UV1 Pump Sampler f UV2A Sampler Column f UV2B Sampler Column f UV3 Column VWD/ f UV4 VWD/ DC Box/ PRV f r UV5 VWD/ PRV f Table 16 Preparative Fraction Collector tubing kits (G1364E) P/N Preparative Fraction Collector tubing kit G G G Coent 4 8 ml/min ml/min 22
23 23
24 Standard Mass-Based Systems Configuration Table 17 Capillary Kit Prep UV/LC Overview System Capillary Kit Prep LC/UV kit for each flow rate Flow range 4 8mL/min ml/min 80 From To Length /cm Fittings UV1 Pump Sampler f UV2A Sampler Column f UV2B Sampler Column f UV3 Column VWD/ f UV4 VWD/ DC Box/ PRV f r UV5 VWD/ PRV f
25 Table 18 Upgrade Capillary kit MS-Based Prep LC Upgrade Capillary kit MS-Based Prep LC No kit 4 8mL/min ml/min 80 1 kit only From To Length /cm ID / No. ID / No. ID / No. ID / No. Fittings MS1B Column Flow modul r r MS2 Flow modul. VWD/ f MS3 Make up Flow modul FLOW INDEPENDENT CAPILLARIES MS4 Flow modul. MS MS5 MS restriction capillary MS6 MS waste capillary NON Table 19 Preparative Open-Bed Fraction Collector tubing kits (G7159B) P/N Preparative Open-Bed Fraction Collector tubing kit Tubing Kit 8 (G ) Tubing Kit 50 (G ) Tubing Kit 150 (G ) Tubing Kit 200 (G ) Suitable for the flow up to 1 8 ml/min ml/min ml/min
26 Table 20 Preparative Fraction Collector tubing kits (G1364E) P/N Preparative Fraction Collector tubing kit G G G Coent 4 8 ml/min ml/min Length/cm No. Fittings FM FM
27 27
28 Configurations with 1290 Infinity II Preparative Column Compartment (G7163B) Entry Level System Configurations (with Manual Injector) Entry Level System Configuration Table 21 Capillary Kit Prep UV/LC Overview System Capillary Kit Prep LC/UV kit for each flow rate Flow range 4 8mL/min ml/min 80 From To Length /cm Fittings UV1 Pump Sampler f UV2A Sampler Column f UV2B Sampler Column f UV2C Sampler Column f UV2D Sampler Column f UV3 Column VWD/ f UV4 VWD/ DC Box/ PRV f r
29 Table 22 Preparative Open-Bed Fraction Collector tubing kits (G7159B) P/N Preparative Open-Bed Fraction Collector tubing kit Tubing Kit 8 (G ) Tubing Kit 50 (G ) Tubing Kit 150 (G ) Tubing Kit 200 (G ) Suitable for the flow up to 1 8 ml/min ml/min ml/min 150 Table 23 Preparative Fraction Collector tubing kits (G1364E) P/N Preparative Fraction Collector tubing kit G G G Coent 4 8 ml/min ml/min 29
30 30
31 Entry Level System Configuration with Delay Coil Organizer (G9324A) Table 24 Capillary Kit Prep UV/LC Overview System Capillary Kit Prep LC/UV kit for each flow rate Flow range 4 8mL/min ml/min 80 From To Length /cm Fittings UV1 Pump Sampler f UV2A Sampler Column f UV2B Sampler Column f UV2C Sampler Column f UV2D Sampler Column f UV3 Column VWD/ f UV4 VWD/ DC Box/ PRV f r Table 25 Preparative Open-Bed Fraction Collector tubing kits (G7159B) P/N Preparative Open-Bed Fraction Collector tubing kit Tubing Kit 8 (G ) Tubing Kit 50 (G ) Tubing Kit 150 (G ) Tubing Kit 200 (G ) Suitable for the flow up to 1 8 ml/min ml/min ml/min
32 Table 26 Preparative Fraction Collector tubing kits (G1364E) P/N Preparative Fraction Collector tubing kit G G G Coent 4 8 ml/min ml/min 32
33 33
34 Standard UV/LC System Configurations Standard UV/LC System Configurations Table 27 Capillary Kit Prep UV/LC Overview System Capillary Kit Prep LC/UV kit for each flow rate Flow range 4 8mL/min ml/min 80 From To Length /cm Fittings UV1 Pump Sampler f UV2A Sampler Column f UV2B Sampler Column f UV2C Sampler Column f UV2D Sampler Column f UV3 Column VWD/ f UV4 VWD/ DC Box/ PRV f r
35 Table 28 Preparative Open-Bed Fraction Collector tubing kits (G7159B) P/N Preparative Open-Bed Fraction Collector tubing kit Tubing Kit 8 (G ) Tubing Kit 50 (G ) Tubing Kit 150 (G ) Tubing Kit 200 (G ) Suitable for the flow up to 1 8 ml/min ml/min ml/min 150 Table 29 Preparative Fraction Collector tubing kits (G1364E) P/N Preparative Fraction Collector tubing kit G G G Coent 4 8 ml/min ml/min 35
36 36
37 Standard UV/LC System Configurations with Delay Coil Organizer (G9328A) Table 30 Capillary Kit Prep UV/LC Overview System Capillary Kit Prep LC/UV kit for each flow rate Flow range 4 8mL/min ml/min 80 From To Length /cm Fittings UV1 Pump Sampler f UV2A Sampler Column f UV2B Sampler Column f UV2C Sampler Column f UV2D Sampler Column f UV3 Column VWD/ f UV4 VWD/ DC Box/ PRV f r Table 31 Preparative Open-Bed Fraction Collector tubing kits (G7159B) P/N Preparative Open-Bed Fraction Collector tubing kit Tubing Kit 8 (G ) Tubing Kit 50 (G ) Tubing Kit 150 (G ) Tubing Kit 200 (G ) Suitable for the flow up to 1 8 ml/min ml/min ml/min
38 Table 32 Preparative Fraction Collector tubing kits (G1364E) P/N Preparative Fraction Collector tubing kit G G G Coent 4 8 ml/min ml/min 38
39 39
40 Standard Mass-Based System Configurations Table 33 Capillary Kit Prep UV/LC Overview System Capillary Kit Prep LC/UV kit for each flow rate Flow range 4 8mL/min ml/min 80 From To Length /cm Fittings UV1 Pump Sampler f UV2A Sampler Column f UV2B Sampler Column f UV2C Sampler Column f UV2D Sampler Column f UV3 Column VWD/ f UV4 VWD/ DC Box/ PRV f r
41 Table 34 Upgrade Capillary kit MS-Based Prep LC Upgrade Capillary kit MS-Based Prep LC No kit 4 8mL/min ml/min 80 1 kit only From To Length /cm ID / No. ID / No. ID / No. ID / No. Fittings MS1A Column Flow modul f MS2 Flow modul. VWD/ f MS3 Make up Flow modul FLOW INDEPENDENT CAPILLARIES MS4 Flow modul. MS MS5 MS restriction capillary MS6 MS waste capillary NON Table 35 Preparative Open-Bed Fraction Collector tubing kits (G7159B) P/N Preparative Open-Bed Fraction Collector tubing kit Tubing Kit 8 (G ) Tubing Kit 50 (G ) Tubing Kit 150 (G ) Tubing Kit 200 (G ) Suitable for the flow up to 1 8 ml/min ml/min ml/min
42 Table 36 Preparative Fraction Collector tubing kits (G1364E) P/N Preparative Fraction Collector tubing kit G G G Coent 4 8 ml/min ml/min Length/cm No. Fittings FM FM
43 43
44 Accessories UV Accessories Table 37 Accessories for kits , , , A-Line Quick Turn LC Fitting for Column 2 pcs for kits and UHP-FF FITTING for Column 2 pcs for kits and m Tube ESD PTFE OD2.5 ID ESD-Fitting OD Union Flat-Bottom 1/4-28 ESD Plastic and PEEK tubing cutter Fitting-Handle Sealtight-Handle MS Accessories Table 38 Accessories 3 m Tube ESD PTFE OD 2.5 ID ESD-Fitting OD Fitting adapter to SST TEE-LOW DEAD VOLUME Fitting-Handle Sealtight-Handle NOTE The flow modulator bypass is part of the G Flow Modulator Accessory Kit. *G * *G * G Number: G Edition: 11/2017 Printed in Germany Agilent Technologies, Inc 2017 Agilent Technologies, Inc Hewlett-Packard-Strasse Waldbronn, Germany
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