ADVANCED IMMUNOLOGY COURSE
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1 ADVANCED IMMUNOLOGY COURSE MASTERS (M2) OF UNIVERSITIES PARIS DESCARTES, PIERRE ET MARIE CURIE ET DIDEROT PROGRAM FROM NOVEMBER 13TH 2017 TO JANUARY 9TH 2018
2 ADVANCED IMMUNOLOGY COURSE MASTERS (M2) des UNIVERSITÉS PARIS DESCARTES, PIERRE et MARIE CURIE et DIDEROT THEMES Development of the immune system. Molecular basis for antigen recognition by B and T lymphocytes. Repertoire selection and auto-immunity. Lymphocyte interactions Immune responses and immunotherapy Innate immune system The course will take place at Education center - Pavillon Louis MARTIN - Building N 9 Centre d Immunologie Humaine - Metchnikoff Building Centre d Immunologie Humaine - François Jacob Building LECTURES AND SEMINARS : Bât. Social Room 4 & Pavillon Louis MARTIN Room 2 PRACTICAL COURSES : Salle TP - RDC 28, rue du Docteur Roux Paris Cedex 15
3 MONDAY 13 TH NOVEMBER 8:30-10:00 Presentation Ana CUMANO, Milena HASAN, Andrés ALCOVER NOVEMBER 13 TH TO DECEMBER 3 TH 2017 Week 1-3 PRACTICAL COURSES ************* DECEMBER 4 TH TO DECEMBER 15 TH 2017 WEEK 3-4 CONFERENCES ************* DECEMBER 19 TH TO DECEMBER 21 TH 2017 BIBLIOGRAPHY SEMINARS ************* JANUARY 9 TH 2018 EXAM *************
4 ADVANCED IMMUNOLOGY PRACTICAL COURSES PROGRAM NOVEMBER 13 TH TO DECEMBER 3 TH 2017 Week 1-3 Application of cutting edge technologies in the characterization of age-dependent differences in the immune response of healthy individuals (supported in part by Milieu Interieur LabEx) Director: Milena Hasan Conferences associated to the Practical Courses: THURSDAY 23 NOVEMBER 9:00-10:00 From Translational Research to Personalized Medicine Stanislas POL (Institut Pasteur / Hôpital Cochin - Paris) TUESDAY 28 NOVEMBER 9:00-10:00 Genetic determinants of the Immune response Lluis QUINTANA-MURCI Designed and organized by Murielle ALMOUSSA, Bernd JAGLA, Bruno CHARBIT Milena HASAN, Isabelle LEQUEUTRE, Valentina LIBRI, Sophie NOVAULT, Sandrine SCHMUTZ
5 Week 1 MONDAY, NOVEMBER 13 8:30-09:30 Reception of students and INTRODUCTION TO THE COURSE 9:30-10:00 Presentation of students 10:00-11:00 INTRODUCTION TO THE PRACTICAL WORK 11:00-11:15 Human samples for research (Estelle Mottez) 11:30-12:00 Presentation of security rules 12:15-13:00 lunch 13:00-14:00 SEMINAR CYTOMETRY 14:00-18:00 Reception of blood; prepare reagents, cytometry staining TUESDAY, NOVEMBER 14 9:00-10:00 Set up of the cytometer 10:00-13:00 Sample acquisition 13:00-14:00 lunch 14:00-18:00 FlowJo data analysis 09:00-13:00 FlowJo data analysis 13:00-14:00 lunch 14:00-15:00 Set up of the cytometer 15:00-18:00 Sample acquisition WEDNESDAY, NOVEMBER 15 09:00-09:30 Introduction 09:30-13:00 Reception of blood; PBMC isolation, cell counting, freezing down of PBMC, immune stimulation of PBMC 13:00-14:00 lunch 14:00-15:15 Run remaining samples on cytometer 15:15-16:30 Run remaining samples on cytometer 14:00-18:00 FlowJo data analysis THURSDAY, NOVEMBER 16 9:00-10:30 Finalize FlowJo data analysis 10:30-12:30 Collection of supernatants after O/N stimulation; Freezing of supernatants and of cell pellets 12:30-13:30 lunch 13:30-18:00 Stimulation and staining of PBMC for imaging cytometry
6 FRIDAY, NOVEMBER 17 9:00-10:00 SEMINAR SEQUENCING 10:00-19:00 Extraction of RNA, quantification of RNA, library preparation for mrna sequencing
7 Week 2 MONDAY, NOVEMBER 20 Group A Group B Group A Group B 9:00-10:00 Put in culture PBMC for Seahorse & Cell Sorting 10:00-11:00 SEMINAR ISX 11:00-13:30 ISX experiment 11:00-13:30 Prepare article for Journal Club 13:30-14:30 lunch 14:30-17:00 Prepare article for Journal Club 14:30-17:00 ISX experiment 9:00-09:30 SEMINAR ELISA/MAGPIX 9:30-11:30 MagPix 11:30-13:00 lunch 13:00-17:00 MagPix TUESDAY, NOVEMBER 21 Group C Group D Group D Group C 9:00-10:00 SEMINAR SORTING 10:00-11:00 Immune stimulation of PBMC for Seahorse & Cell sorting 11:00-11:30 SEMINAR ELISA/MAGPIX 11:30-13:00 MagPix 13:00-14:30 lunch 14:30-18:30 MagPix 10:00-11:00 SEMINAR ISX 11:00-12:00 Put in culture PBMC for Seahorse & Cell sorting 12:00-13:00 lunch 13:00-15:30 ISX experiment 13:00-15:30 Prepare article for Journal Club 15:30-18:00 ISX experiment 15:30-18:00 Prepare article for Journal Club 18:30-19:00 Collection of supernatants for ELISA 19:00-20:00 MagPix data analysis
8 WEDNESDAY, NOVEMBER 22 9:00-10:00 - SEMINAR From translational research to (Stanislas POL) personalized medicine 10:00-11:00 SEMINAR SEAHORSE Group A 11:00-13:30 Seahorse experiment Group B 11:00-13:30 Report writing/prepare article for Journal Club 13:30-14:30 lunch Group B 14:30-17:00 Seahorse experiment Group A 14:30-17:00 Report writing/prepare article for Journal Club 17:00-18:00 MagPix data analysis 10:00-11:00 Immune stimulation of PBMC for Seahorse & Cell sorting 11:00-13:00 Staining of cells for sorting 13:00-14:00 lunch Group C 14:00-16:30 Single-cell sorting Single-cell RT+ preamplification Group D 14:00-16:30 Report writing/prepare article for Journal Club Group D 16:30-19:00 Single-cell sorting Single-cell RT+ preamplification Group C 16:30-19:00 Report writing/prepare article for Journal Club THURSDAY, NOVEMBER 23 Group B Group A+D Group A+D Group A Group B+C Group B+C Group C Group D 9:00-10:30 Staining of cells for sorting 10:30-12:30 Single-cell sorting Single-cell RT+ preamplification 10:30-13:30 Reverse transcription +PCR+ preparation of gel 13:30-14:30 lunch 14:30-16:30 Single-cell sorting Single-cell RT+ preamplification 12:30-13:30 lunch 14:00-17:00 Reverse transcription +PCR+ preparation of gel 09:00-10:00 SEMINAR SEAHORSE 10:30-13:00 Seahorse experiment 14:30-17:00 Seahorse experiment 17:00-18:00 Seahorse data analysis FRIDAY, NOVEMBER 24 09:00-18:00 RNA sequencing - data analysis
9 Week 3 MONDAY, NOVEMBER 27 9:00-18:00 - RNA sequencing - data analysis TUESDAY, NOVEMBER 28 9:00-10:00 - SEMINAR Genetic determinants of (Lluis QUINTANA MURCI) the Immune response Group C Group D Group A Group B 10:00-11:00 SEMINAR BIOMARK 11:00-12:00 Biomark experiment 12:00-13:30 Biomark experiment 13:30-15:00 Biomark experiment 15:00-16:30 Biomark experiment 16:30-18:00 Biomark experiment 12:00-15:00 Separation of amplified fragments on agarose gel 15:00-18:00 Separation of amplified fragments on agarose gel WEDNESDAY, NOVEMBER 29 09:00-12:00 ISX data analysis 12:00-13:00 lunch 13:00-14:00 Coating of plates for ELISA 14:00-18:00 BIOMARK data analysis THURSDAY, NOVEMBER 30 09:00-16:30 Classical ELISA FRIDAY, DECEMBER 1 Group C Group D Group B Group A 9:00-12:00 Report writing 12:00-13:00 lunch 13:00-13:30 Present an article: Journal Club 13:30-14:00 Present an article: Journal Club 14:00-14:30 Present an article: Journal Club 14:30-15:00 Present an article: Journal Club 15:00 farewell party Dead-line for submission of the reports: Sunday, December 3, at midnight
10 Week 4 ADVANCED IMMUNOLOGY CONFERENCES FROM MONDAY DECEMBER 4 TH TO FRIDAY DECEMBER 15 TH 2017 MODULE D ECOLE DOCTORALE ADVANCED IMMUNOLOGY -1 MONDAY, DECEMBER 4 9:00-12:00 Phylogeny of the Immune System Louis du PASQUIER (University of Bale, Switzerland) 13:30-16:30 Stem cells, hematopoiesis and lymphopoiesis Ana CUMANO TUESDAY, DECEMBER 5 9:00-12:00 Origin of antigen receptor diversity Ludovic DERIANO and recombination 13:30-16:30 Thymus development Thomas BOEHM (Max-Planck Institute - Freiburg, Germany) WEDNESDAY, DECEMBER 6 9:00-12:00 Commensals, the Immune System and the Brain Gerard EBERL 13:30-16:30 Lymphocyte dynamics. Philippe BOUSSO THURSDAY, DECEMBER 7 9:00-12:00 Heterogeneity of T helper cell subsets. Marc VELDHOEN (Institute of Molecular Medicine, Lisbon, Portugal) 13:30-16:30 Peripheral tolerance and autoimmunity. Burkhard BECHER (University of Zurich - Switzerland) FRIDAY, DECEMBER 8 9:00-12:00 α/β T cell development and selection Ludger KLEIN (Institute for Immunology, Munich) 13:30-16:30 γ /δ T cells : development and function. Adrian HAYDAY (Francis Crick Institute - London, UK)
11 Week 5 ADVANCED IMMUNOLOGY CONFERENCES MODULE D ECOLE DOCTORALE ADVANCED IMMUNOLOGY -2 MONDAY, DECEMBER 11 9:00-12:00 Vaccines Philippe SANSONETTI 13:30-16:30 Lymphocyte activation and Immunological Synapses Andrés ALCOVER TUESDAY, DECEMBER 12 9:00-12:00 Lymphoid tissue development. Tom CUPEDO (Erasmus University, Rotterdam, The Netherlands) 13:30-16:30 Dendritic cells Philippe PIERRE (Centre d Immunologie Marseille-Luminy) WEDNESDAY, DECEMBER 13 9:00-12:00 Innate lymphoid cells James DI SANTO 13:30-16:30 Leukemia pathogenesis Camille LOBRY (Institut Gustave Roussy - Villejuif) THURSDAY, DECEMBER 14 9:00-12:00 Molecular Basis of Immune-deficient diseases Alain FISCHER (Institut Imagine - Paris) 13:30-16:30 Fc receptors and allergy Marc DAERON (Centre d Immunologie Marseille-Luminy) FRIDAY, DECEMBER 15 9:00-12:00 Control of Disease Susceptibility Miguel SOARES (Gulbenkian Institute - Oeiras, Portugal) 13:30-16:30 B cell memory Thierry DEFRANCE (Center for Infectiology Res., Lyon)
12 Week 6 ADVANCED IMMUNOLOGY BIBLIOGRAPHY SEMINARS TUESDAY, DECEMBER 19 9:30-12:30 SEMINAR 1 Antibodies in health and disease Organizers : Friederique JÖNSSON Hugo MOUQUET 14:00-17:00 SEMINAR 2 Lymphocyte Trafficking Organizers: Idan MILO Emmanuel DONNADIEU (Institut Cochin - Paris) WEDNESDAY, DECEMBER 20 9:30-12:30 SEMINAR 3 Lymphocyte activation and transcriptional regulation. Organizers: Elisabetta BIANCHI Jerome DELON (Institut Cochin - Paris) 14:00-17:00 SEMINAR 4 T cell responses. Organizers: Corinne TANCHOT (Hôpital Européen Georges Pompidou - Paris) Christine BOURGEOIS (Faculté de Médecine.- Université Paris Sud) THURSDAY, DECEMBER 21 9:30-12:30 SEMINAR 5 Innate immunity Organizers: Jean-Pierre LEVRAUD Florence NIEDERGANG (Institut Cochin - Paris) 14:00-17:00 SEMINAR 6 Molecular basis of B cell differentiation. Organizers: Paulo VIEIRA (Institut Pasteur- Paris) Isabelle ANDRÉ-SCHMUTZ (Institut Imagine - Paris) 17:30 PARTY
13 TUESDAY JANUARY 9 TH, :00-13:00 EXAM: AMPHITHEATRE DUCLAUX 14:30-17:30 Answers to the Exam s questions interactive feed back session Salle Metchnikoff - RdC
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