49 Woodside Street Stamford, CT 06902

Transcription

1 Environmental Consultants And Laboratory Services 11 HYGENIX, INC. 49 Woodside Street Stamford, CT (203) Fax (203) Westport Public Schools Attn. Craig Schmarr 110 Myrtle Ave Westport, CT September 29, 2018 Re: Post Remediation Inspection Coleytown Middle School Dear Mr. Schmarr. This letter summarizes the post remediation inspection and sampling performed at the above referenced school on September 27, 2018.At the time of the inspection, Rooms 113,203, 208 appeared to be clean and dry. To quantify for airborne mold, indoor air samples and one outdoor reference air sample were collected using Zefon Air-0-Cell cassettes in accordance with the manufacturer's recommended protocol. All samples were delivered to Pure Earth Environmental Laboratory Inc., where they were analyzed for mold by dlrect microscopic examination. The following table is a summary of the attached lab results: TABLE 1. AIR-0-CELL SAMPLE RESULTS Location Total Counts Predominant Species Individual Counts pr/m Spr/m3 -- _ Outdoors Room 203 Room 208 Room 113 j Ascospores 4373 Basidiospo es 1173 As co spores 160 Cladosporiu_ m Ascospores 587. Basid ospores 267 Ascospores 213 I Cladosporium 160 The following typical indoor mold spore concentration ranges were suggested as a "guide to evaluating the relative degree of indoor airborne mold spore amplification'' by Daniel Baxter, the inventor of the Air-0-Cell mold spore trap, the most widely used b;oaerosol sampling device: TABLE Desc ription Spores( CTS/M3) Predominan! Types "Clean" building.. less tha _ n 2,000. Total for all spores less than 700 Penicillium,A, _ f!rgi/jus Possible Indoor Amplifi _ a _ ion 1,000-5,000 Penicillium, Aspergillus, Cladosporium Indoor Amplification likely present 5,000-10, h ronic Indoor Amplification 10, ,000 Penicillium, Aspergi/Jus, Cladosporium _ nadequate flood cleanup or active 50,000-10,000,000 Penicillium, Aspergil/us, Stachybotrys, indoor demolition of contaminated Cladosporium, Chaetomi/Jm, Basidiomycete s, l surfaces Trichoderma, Ulocladium, etc.

2 Westport Public Schools Coleytown Middle School September 2018 Room 203, 208, Airborne Mold Test- Satisfactory -The sample results were not elevated and have satisfied the criteria for "Clean" building. Room 203,208,113 -Visual Inspection -Satisfactory-All areas/surfaces appeared to be clean and dry. Work area containment have met post remediation standards.

3 Westport Public Schools Coleytown Middle School July 2018 AIR TEST LAB RESULTS

4 - Arnobioloqy ASSOCIATES, L1'boRAroRy INCOf{PORi\TfD Expertise Since 1997 Certificate of Analysis AIHA-LAP EMLAP# North Park Drive Pennsauken, New Jersey (856) Hygenix, Inc 49 Woodside St. Stamford, Connecticut Project: HYGENIX WPS/ CMS 0927 Condition of Sample( s) Upon Receipt: Acceptable Date Collected: 09/27/2018 Date Received: 09/28/2018 Date Analyzed: 09/28/2018 Date Reported: 09/28/2018 Project ID: Page 1 of Spore Trap Analysis: SOP 3 8 Client Sample Number Sample Location Sample Volume (L) Lab Sample Number Spore Identification ascospores basidiospores Cladosporium hyphal elements Smuts,Periconia,Myxomycetes I % Ttl I In/Out Raw Ctl Raw Ctl... spr/m 3 3 I 1 eo I 30 l 1121 a2 l z 101 s I so I I 1 I - l I - I - 2 I OUTDOOR spr/m I 3 % Ttl I In/Out 4373 I ee a 640 I 10 I - s I 2 I - Analytical Sensitivity Analytical Sensitivity: 13 spr/m 3 Analytical Sensitivity: 13 spr/m 3 Comments L - al *See Footnotes 10 l 533 l-100%j I 634_ _J-100%I Client Sample Number Sample Location 208 OUTDOOR Sample Volume {L) Lab Sample Number Spore Identification Raw Ct[ spr/m 3 I % Ttl Un/Out Raw CtJ spr/m 3 I %Ttl In/Out ascospores 11 I 587 l 61 I 1/7 82 l 4373 I 69 - basidiospores 5 I 267! 28 I 1/4 22 I 1173 I 18 I - Cladosporium 2 I 107 I 11 I 1/6 12 I 640 I 10 I - hyphal elements - I - I - I - 1 I 53 I 1 I - Smuts,Periconia,Myxomycetes - l - I - I - 2 I 107 I 2 I Analytical Sensitivity Analytical Sensitivity: 13 spr/m 3 Analytical Sensitivity: 13 spr/m 3 Comments Total *See Footnotes 18 I %1 1/7 119 I % -

5 '-- AERobioloqy A.%00.\TES, L,boRAIORY INCORPORATED Expertise Since 1997 Certificate of Analysis AIHA-LAP EMLAP# North Park Drive Pennsauken, New Jersey (856) Hygenix, Inc 49 Woodside St. Stamford, Connecticut Project: HYGENIX WPS/ CMS 0927 Condition of Sample(s) Upon Receipt: Acceptable Date Collected: 09/27/2018 Date Received: 09/28/2018 Date Analyzed: 09/28/2018 Date Reported: 09/28/2018 Project ID: Page 2 of 3 Client Sample Number Sample Location 113 Sample Volume (L) 75 Lab Sample Number Spore Identification Raw Ctl spr/m 3 I %TII! In/Out ascospores 4, I 36 I 1121 basidiospores - I - - I - Cladosporium 3 I 160 I 27 I 1/4 hyphal elements - I - I - I - Penicillium/Aspergillus group 3 I I - Smuts,Periconia,Myxomycetes 1 I 53 9 I 1/2 Analytical Sensitivity Analytical Sensitivity: 13 spr/m 3 Comments Total *See Footnotes 11 I %1. 1/ OUTDOOR Raw Ct! spr/m 3 I % Ttl I In/Out 82 I 4373 I 69 I - 22 I 1173 I 18 I - 12 I 640 I 10 I - 1 I 53 I 1 I - - I - I - I - 2 I 107 I 2 I - Analytical Sensitivity: 13 spr/m I %1 -

6 . Anmbioloqy labor<atory ASSOCIATES, INCORPORATED Expertise Since 7997 Certificate of Analysis AIHA-LAP EMLAP# North Park Drive Pennsauken, New Jersey (856) Hygenix, Inc Date Collected: Date Received: 49 Woodside St. Date Analyzed: Stamford, Connecticut Project: HYGENIX WPS/ CMS 0927 Condition of Sample(s) Upon Receipt: Acceptable Date Reported: Project ID: 09/27/ /28/ /28/ /28/ Page 3 of 3 I Footnotes and Additional Report Information Debris Rating -Table 1 Minimal (<5%) present Reported values are minimally affected by load % to 25% of the trace occluded with Negative bias is expected. The degree of bias increases directly with the percent of the trace that is occluded. 26% to 75% of the trace occluded with Negative bias is expected. The degree of bias increases directly with the percent of the trace that is occluded. 75% to 90% of the trace occluded with Negative bias is expected. The degree of bias increases directly with the percent of the trace that is occluded. Quantification not possible due to large negative bias. A new sample should be Greater than 90% of the trace occluded with 5 collected at a shorter time interval or other measures taken to reduce load Penicillium/Aspergillus group spores are characterized by their small size, round to ovoid shape, being unicellular, and usually colorless to lightly pigmented. There are numerous genera of fungi whose spore morphology is similar to that of the Penicillium/Aspergillus type. Two common examples would be Paecilomyces and Acremonium. Although the majority of spores placed in this group are Penicillium, Aspergillus, or a combination of both. Keep in mind that these are not the only two possibilities. 2. Ascospores are sexually produced fungal spores formed within an ascus. An ascus is a sac-like structure designed to discharge the ascospores into the environment, e.g. Ascobolus. 3. Basidiospores are typically blown indoors from outdoors and rarely have an indoor source. However, in certain situations a high basidiospore count indoors may be indicative of a wood decay problem or wet soil. 4. The colorless group contains colorless spores which were unidentifiable to a specific genus. Examples of this group include Acremonium, Aphanocladium, Beauveria, Chrysosporium, Engyodontium microconidia, yeast, some arthrospores, as well as many others. 5. Hyphae are the vegetative mode of fungi. Hyphal elements are fragments of individual Hyphae. They can break apart and become airborne much like spores and are potentially allergenic. A mass of hyphal elements is termed the mycelium. Hyphae in high concentration may be indicative of colonization. 6. Dash (-) in this report, under raw count column means 'not detected (ND)'; otherwise 'not applicable' (NA). 7. The positive-hole correction factor is a statistical tool which calculates a probable count from the raw count, taking into consideration that multiple particles can impact on the same hole; for this reason the sum of the calculated counts may be less than the positive hate corrected total. 8. Due to rounding totals may not equal 100%. 9. Analytical Sensitivity for each spores is different for Non-viable sample when the spores are read at different percentage. Analytical Sensitivity is calculated as spr/m 3 divided by raw count. spr/m 3 = raw counts x (100/ % read) x (1000/Sample volume). If Analytical Sensitivity is 13 spr/m 3 at 100% read, Analytical Sensitivity at 50% read would be 27 spr/m 3, which is 2 times higher. Analytical Sensitivity provided on the report is based on an assumed 100% of the trace being analyzed. 10. Minimum Reporting Limits (MRL) for BULKS, DUSTS, SWABS, and WATER samples are a calculation based on the sample size and the dilution plate on which the organism was counted. Results are a compilation of counts taken from multiple dilutions and multiple medias. This means that every genus of fungi or bacteria recovered can be counted on the plate on which it is best represented. 11. If the final quantitative result is corrected for contamination based on the blank, the blank correction is stated f n the sample comments section of the report. 12. The results in this report are related to this project and these samples only. 13. For samples vvith an air volume of < 1 OOL, the number of significant figures in the result should be considered (2) two. For samples with air volumes between l, the number of significant figures in the result should considered (3) three. For example, a sample with a result of 55,443 spr/m 3 from a 75L sample using significant figures should be considered 55,000. The same result of 55,443 from a 150L sample using significant figures should be considered 55,400 spr/m If the In/Out ratio is greater than 100 times it is indicated >100/1, rather than showing the real value. Terminology Used in Direct Exam Reporting Conidiophores are a type of modified hyphae from which spores are born. When seen on a surface sample in moderate to numerous concentrations they may be indicative of fungal growth...- «= -:p,.,f... :,_.,.,.). Q Suzanne S. Blevins, B.S., SM (ASCP) Laboratory Director 2018 Aerobiology Laboratory Associates 1 In. All rightli re erved.

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