INDOOR AIR QUALITY CLEARANCE SAMPLING

Transcription

1 5 Route, Suite C4 Brewster, NY State Street, Suite 700 Albany, NY Wehrle Drive, Suite One Buffalo, NY adelaid @adelaidellc.com Fax: IOOR AIR QUALITY CLEARANCE SAMPLING PERFORMED AT: Adelaide Project#YONK:89.0-PM PREPARED FOR: Yonkers Public Schools One Larkin Center Yonkers, New York 070 PREPARED BY: Jason Fullum October 9, 08 REVIEWED BY: Stephanie A. Soter President Over Three Decades of Service Woman-Owned Business Enterprise GSA Contract GS-0F-0080V Asbestos and Lead Consulting Hazardous Material Investigations Mold/Bacteria Consulting Indoor Air Quality Assessments Industrial Hygiene Investigations

2 TABLE OF CONTENTS.0 Introduction. Scope of Work / Project Personnel. Executive Summary.. Conclusions and Recommendations.. Indoor Air Quality. Observations.0 Sampling Methodology.0 Disclaimers APPEICES Sample Location Map(s) Analytical Results Personnel and Laboratory Certifications A B C

3 .0 Introduction. Scope of Work / Project Personnel (Adelaide) performed an Indoor Air Quality Clearance Sampling, in conformance with ALL Federal, State and Local regulations, on October, 08 for Yonkers Public Schools throughout all rooms located at in Yonkers, New York. The clearance sampling included ) a visual inspection/assessment throughout accessible interior and/or exterior spaces of the building/structure identified to be affected; and, ) collecting of various air, tape, swab and/or bulk sample(s) at the discretion of the mold assessor; Certified Adelaide personnel (Appendix C), Jason Fullum (NYS Mold Assessor/Cert. #MA00056), performed the visual assessment throughout affected area(s) identified.. Executive Summary Following the scope of work that was provided to us, Adelaide performed an indoor air quality clearance sampling of all the rooms on all floors for post abatement clearance. Adelaide collected one hundred and thirty five (5) air samples from the above-mentioned area(s). For testing locations, refer to the sample location maps (Appendix A)... Conclusions and Recommendations The following conclusions and recommendations are prepared by Adelaide as per the provided scope of work. Should the scope of work change, it is recommended that the findings be revisited to determine if additional sampling will be required to satisfy ALL Federal, State and Local regulations... Indoor Air Quality Air samples were collected in each space throughout the basement, first, second and third floors of the building after mold abatement was completed. The abatement included removals of the visible mold, removal of water stained areas and fogging of the duct work to kill any mold that may be remaining. The air samples all showed acceptable levels and the area can be reoccupied. Even though Stachybotrys was found in the air samples in rooms 06, storage room A, st floor hallway south and room 09 it was a minimal amount of one or two spores at the highest.. Observations The following observations were made during the assessment: No visible mold was observed during the final clearance. ADELAIDE ENVIRONMENTAL HEALTH ASSOCIATES, INC. ADELAIDE PROJECT # YONK:89.0-PM Indoor Air Quality/Mold Assessment

4 .0 Sampling Methodology Yeasts and Molds: Adelaide uses 4.4mm,.7 micron Air-O-Cell cassettes at 5.0L/min to sample airborne mold. Samples of unused filters, handled in the same manner as the sample cassettes, are analyzed as blanks to ensure no contamination was from the process of taking the sample. The filters from the samples are then diluted and redeposited on malt extract agar (for fungi and mold) and tryptic soy agar (for bacteria). The agar is then incubated from four to seven days. The growth is both counted and identified. This is very important. "s", alone, are only half the story. The other half is whether the mold present is an opportunistic pathogen or just an allergen. Yeast and mold concentrations vary outdoors based on rainfall, temperature, vegetation, soil disturbance, wind and other factors. There are no "regulatory standard" methods at this time. The method used was provided by Dr. Chin Yang, Consulting Mycologist, formerly with the U.S. Public Health Service, Division of Federal Occupational Health. Bulk samples collected are sent to the laboratory in a 4 mil poly bag. The sample(s) received by the laboratory are matched with the information provided on the Chain of Custody (COC). A laboratory Identification Number is assigned and the project and sample information is logged. Analysis begins with an inspection of the bulk material and any areas of discoloration or potential fungal presence are noted. Clear tape is used to take representative samples from the material and the tape(s) are reviewed microscopically and any detected fungi are identified and estimated amounts are noted in our reporting system. In evaluating microbiological test data (air samples, wipes, swipes, vacuum swipes, swabs, etc.), there are several assumptions and guidelines we follow, and we list them below:. Our reference guide for interpreting microbiological analytical results is the Proceedings of the International Conference of Fungi and Bacteria in Indoor Air Environments, Edited by Drs. Eckardt Johanning and Chin S. Yang. Additional references include: a. Aerobiology, edited by Muilenberg and Burge, CRC Press (996) b. Field Guide for the Determination of Biological Contaminants in Environmental Samples, Dillon, Heinsohn, Miller, AIHA Publications (996) c. Biosafety Reference Manual, Heinsohn, Jacobs, Concoby, AIHA Publications (995) d. Indoor Air and Human Health, Gammage, Kaye, Lewis Publishers, (985) e. Indoor Air and Human Health Second Edition, Gammage, Berven, CRC Press (996). The values we use to interpret air sample data was provided by Dr. Chin Yang. He is a consulting Mycologist, and presently performs consulting work for the U.S. Public Health Service concerning Bioaerosols and Indoor Air Quality. Low: Moderate: High: 0 to 00 CFU/m (limited or no effect level) 00 to 50 CFU/M (minor effect level; persons with hypersensitivity, allergies or immunosuppressed may experience an "effect".) 50 to 000 CFU/m (effect level for the average or normal healthy adult; the effects vary from minor discomfort to lost job time) ADELAIDE ENVIRONMENTAL HEALTH ASSOCIATES, INC. ADELAIDE PROJECT # YONK:89.0-PM Indoor Air Quality/Mold Assessment

5 Very High: 000 CFU/m and above (noticeable odor, growth, illness etc.; a decontamination strategy is normally required.) The levels are guideline levels. There is NO law or legal requirement to do anything based on the above levels. They are OPINIONS.. Why perform microbiological testing if it is so inaccurate, the data is subject to "opinion", and there is no legal requirement? Again, all we can offer is another opinion. When it comes to a person's health and well-being, we like having as much data as possible to determine if a person is "at risk" from the conditions in the workplace. Microbiological testing provides enough data to determine if there is a potential risk from exposure to bioaerosols. When the numbers are above "low", you then take into account the individuals affected, i.e., their medical history, and make a judgment. Although bodies such as the ACGIH do not give numerical guidelines, a Canadian guide on office buildings based on five years of investigation of 50 air-conditioned federal government buildings (Nathanson, 99) includes some guidance on numbers. The following are the main points: a. The "normal" air flora should be quantitatively lower than, but qualitatively similar to, that of outdoor air. b. The presence of one or more fungal species at significant levels in indoor but not outdoor samples is evidence of an indoor amplifier. c. Pathogenic fungi such as Aspergillus fumigatus, Histoplasma and Cryptococcus should not be present in significant numbers. d. The persistence of toxigenic molds such as Stachybotrys atra and Aspergillus versicolor in significant numbers requires investigation /action. e. More than 50 CFU/m (0,000 CFU/g) may be of concern if there is only a single species present (other than certain common outdoor phylloplane fungi); up to 50 CFU/m (0,000 CFU/g) is acceptable if the species present reflect the flora outdoors; up to 500 CFU/m (50,000) is acceptable in summer if outdoor leaf-inhabiting fungi are the main components..0 Disclaimers Adelaide certifies that the information contained within this report is based solely upon site observations and the results of laboratory analysis for samples collected during this survey/assessment. These observations and results are time dependent, subject to changing site conditions and revisions to Federal, State and Local regulations. Adelaide warrants that these findings have been promulgated after being prepared in general accordance with generally accepted practices in the abatement industries. Adelaide also recognizes that inspection laboratory data is not usually sufficient to make all abatement and management decisions. No other warranties are expressed or implied. ADELAIDE ENVIRONMENTAL HEALTH ASSOCIATES, INC. ADELAIDE PROJECT # YONK:89.0-PM Indoor Air Quality/Mold Assessment

6 APPEIX A SAMPLE LOCATION MAP(S)

7 . Version # 0/4/08 Issued For: Mold Survey 5 Route Brewster, NY 0509 Phone: (845) Fax: (845) Adelaide Project NO. YONK:89.0- AQ Drawing Prepared By: JPF Paideia School 5 Yonkers Public Schools One Larkin Center Yonkers, New York 070 Client Project No. First Floor Key Plan - Sample Locations **Drawing Not to Scale** SLM -0

8 . Version # 0/4/08 Issued For: Mold Survey 5 Route Brewster, NY 0509 Phone: (845) Fax: (845) Adelaide Project NO. YONK:89.0- AQ Drawing Prepared By: JPF Paideia School 5 Yonkers Public Schools One Larkin Center Yonkers, New York 070 Client Project No. Second Floor Key Plan - Sample Locations **Drawing Not to Scale** SLM -0

9 . Version # 0/4/08 Issued For: Mold Survey 5 Route Brewster, NY 0509 Phone: (845) Fax: (845) Adelaide Project NO. YONK:89.0- AQ Drawing Prepared By: JPF Paideia School 5 Yonkers Public Schools One Larkin Center Yonkers, New York 070 Client Project No. Third Floor Key Plan - Sample Locations **Drawing Not to Scale** SLM -0

10 . Version # 0/4/08 Issued For: Mold Survey 5 Route Brewster, NY 0509 Phone: (845) Fax: (845) Adelaide Project NO. YONK:89.0- AQ Drawing Prepared By: JPF Paideia School 5 Yonkers Public Schools One Larkin Center Yonkers, New York 070 Client Project No. Basement Floor Key Plan - Sample Locations **Drawing Not to Scale** SLM -04

11 APPEIX B ANALYTICAL RESULTS

12 HMC # Analysis Report prepared for 5 Route Suite #C4 Brewster, NY Phone: (845) Fax: (845) Job Number: 89.0-PM Job Name: Date Sampled: Date Analyzed: Report EPA Laboratory ID# VA049 AIHA EMPAT Lab ID# 8886 Mold License: LAB0 License: #PH-098 Page of

13 HMC # Route Suite #C4 October, 08 Client Job Number: Client Job Name: 89.0-PM Dear, We would like to thank you for trusting Hayes Microbial for your analytical needs. On October, 08 we received 5 samples by FedEx for the job referenced above. 5 samples were received in good condition. The results in this analysis pertain only to this job, collected on the stated date and should not be used in the interpretation of any other job. This report may not be duplicated, except in full, without the written consent of Hayes Microbial Consulting, LLC. This laboratory bears no responsibility for sample collection activities, analytical method limitations, or your use of the test results. Interpretation and use of test results are your responsibility. Any reference to health effects or interpretation of mold levels is strictly the opinion of Hayes Microbial Consulting. In no event, shall Hayes Microbial Consulting or any of its employees be liable for lost profits or any special, incidental or consequential damages arising out of your use of the test results. Steve Hayes, BSMT(ASCP) Laboratory Director Hayes Microbial Consulting, LLC Page of

14 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium Bsmt - Boiler Room by Door spores/m / M Signature: Bsmt - Boiler Room Chiller # spores/m / M 0.9% 7.9% % Common Allergen 0/0/08 Date Collected: Date Received: Date Reported: / M 0/0/08 0/0/08 0/0/ Bsmt - Elevator Machine Room spores/m / M 8.4% % 4.8% 5.8% 0 5.8% 5.% 5.6% 5.7% 7.0% % Slightly Higher than Outside Air HMC # Bsmt - Lobby by Elevator spores/m 5 5 SOP #HMC0 4 9 Water Damage Indicator Job Name: Spore Trap Analysis Reviewed by: Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page of

15 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium st Fl - Gym South spores/m / M Signature: HMC #80467 Date Collected: Date Received: Date Reported: st Fl - Gym North spores/m SOP #HMC0 / M st Fl - Room 06 spores/m / M 0/0/08 0/0/08 0/0/ st Fl - Girls Bathroom spores/m / M %.5% 7 6.9% 9.7% % 0 7.5% % % 7.0% 8.% 8.% Water Damage Indicator Job Name: Spore Trap Analysis.% 0 4 Common Allergen 86 Slightly Higher than Outside Air 0/0/08 Reviewed by: Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page 4 of

16 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium Water Damage Indicator Signature: Job Name: st Fl - Computer Room Hallway spores/m / M SOP #HMC0 HMC #80467 Date Collected: Date Received: Date Reported: st Fl - Storage spores/m Spore Trap Analysis / M st Fl - Boys Bathroom spores/m / M 0/0/08 0/0/08 0/0/ st Fl - Computer Room spores/m / M 4.0%.% 5.0% % % % 8.% % 7 9.0% % 5.% Common Allergen 06 Slightly Higher than Outside Air 0/0/08 Reviewed by: Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page 5 of

17 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium st Fl - Room 046 spores/m / M Signature: st Fl - Storage 5 West spores/m / M 6.7% 4.5% % % 6.7% 67 Common Allergen 89 Slightly Higher than Outside Air 0/0/08 HMC #80467 Date Collected: Date Received: Date Reported: st Fl - Storage 5 East spores/m SOP #HMC0 8 Water Damage Indicator Job Name: Spore Trap Analysis Reviewed by: / M 0/0/08 0/0/08 0/0/ st Fl - Storage A spores/m / M % 5.8% 75.6% 5.% %.4% 60 6.% 5.% Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page 6 of

18 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium st Fl - Comm Room 8 spores/m / M Signature: %.% 8.% % 47 Common Allergen %.9% 7 6.% 44 Slightly Higher than Outside Air 0/0/08 HMC #80467 Date Collected: Date Received: Date Reported: st Fl - Boys Bathroom spores/m 6.% 9.% 5.% 4.% / M SOP #HMC st Fl - Elec Room A spores/m 7/M Water Damage Indicator Job Name: Spore Trap Analysis Reviewed by: / M 0/0/08 0/0/08 0/0/ st Fl - Custodial Closet spores/m /M / M 0.9% 7.7% % % 0.9% 5.8% 5.8% 5.8% Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page 7 of

19 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium Water Damage Indicator Signature: Job Name: st Fl - Girls Bathroom spores/m / M SOP #HMC0 HMC #80467 Date Collected: Date Received: Date Reported: st Fl - Room 0 spores/m Spore Trap Analysis / M st Fl - Room 0 spores/m / M 0/0/08 0/0/08 0/0/ st Fl - Room 05 spores/m / M 4.0% 0.0% 7.9% 8.% % % % % 4.0% 7.5% 5.0% Common Allergen Slightly Higher than Outside Air 0/0/08 Reviewed by: 7 7 Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page 8 of

20 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium st Fl - Room 07 spores/m / M Signature: HMC #80467 Date Collected: Date Received: Date Reported: st Fl - Room 09 spores/m SOP #HMC0 / M st Fl - Hallway S spores/m / M 7.5% % % % % Common Allergen 707 Slightly Higher than Outside Air 0/0/08 Reviewed by: 0/0/08 0/0/08 0/0/ st Fl - Hallway C spores/m Water Damage Indicator Job Name: Spore Trap Analysis / M 69.6% %.% 7.9% 4.% 7 4.6% 4.% Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page 9 of

21 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium Water Damage Indicator Signature: Job Name: st Fl - Hallway N spores/m st Fl - Mech Room spores/m % % % 7.5% 7.% Common Allergen / M Slightly Higher than Outside Air 0/0/08 HMC #80467 Date Collected: Date Received: Date Reported: st Fl - Electric Room spores/m SOP #HMC0.5% 9.0% / M Spore Trap Analysis Reviewed by: / M 0/0/08 0/0/08 0/0/ st Fl - Grands Room spores/m / M % % % % Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page 0 of

22 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium Water Damage Indicator Signature: Job Name: st Fl - Maint. Office spores/m /M / M SOP #HMC0 HMC #80467 Date Collected: Date Received: Date Reported: st Fl - Toilet 08 spores/m Spore Trap Analysis / M st Fl - Toilet 09 spores/m /M / M st Fl - Hallway 05 spores/m % 7 6.9% % % 0 8.% % % < % Common Allergen Slightly Higher than Outside Air 0/0/08 Reviewed by: 0/0/08 0/0/08 0/0/08 Significantly Higher than Outside Air / M % 9.6% 65.% Ratio Abnormality 0/0/08 Page of

23 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium st Fl - Room 0E Staff spores/m / M Signature: HMC #80467 Date Collected: Date Received: Date Reported: st Fl - Cafeteria East spores/m SOP #HMC0 / M st Fl - Cafeteria West spores/m / M 0/0/08 0/0/08 0/0/ st Fl - Kitchen spores/m / M % % % 5.8% % % % 6 84.% < % < % %.8% 7.% Water Damage Indicator Job Name: Spore Trap Analysis 86 Common Allergen < % Slightly Higher than Outside Air 0/0/08 Reviewed by: Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page of

24 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium Water Damage Indicator Signature: Job Name: st Fl - 0J Storage spores/m / M SOP #HMC0 HMC #80467 Date Collected: Date Received: Date Reported: st Fl - Women's Locker spores/m Spore Trap Analysis / M st Fl - Toilet spores/m / M 0/0/08 0/0/08 0/0/ st Fl - Office spores/m / M 6.% %.5% 7 8.7% % 5 8.% % %.% Common Allergen Slightly Higher than Outside Air 0/0/08 Reviewed by: Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page of

25 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium Water Damage Indicator Signature: Job Name: st Fl - Men's Locker spores/m / M SOP #HMC0 HMC #80467 Date Collected: Date Received: Date Reported: st Fl - Receiving spores/m Spore Trap Analysis / M st Fl - Food Storage spores/m / M Exterior - North spores/m /M % 7.6% % % % 8 78.% /0/08 0/0/08 0/0/ < % 7.4%.% 77.5% 6.9% 6 80 < % < % 86 0 / M.4% 7 Common Allergen 7 Slightly Higher than Outside Air 0/0/08 Reviewed by: 07 Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page 4 of

26 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium Exterior - East spores/m /M Signature: Exterior - West spores/m /M % < % 77.8% < % 7 0.% < % 4 5 < % 097 Common Allergen / M / M % < % 78.6% < % < % 60.% 8 07.% 7 < % 7 9 < % 5 67 < % 7 9 < % /0/08 Reviewed by: 0/0/08 0/0/08 0/0/ Field Blank 0 liters 0 spores/m Slightly Higher than Outside Air HMC #80467 Date Collected: Date Received: Date Reported: Exterior - South spores/m 7/M SOP #HMC0 < % 8.7% < % 77.9% / M 8 Water Damage Indicator Job Name: Spore Trap Analysis Significantly Higher than Outside Air / M Ratio Abnormality 0/0/08 Page 5 of

27 Spore Trap Information HMC #80467 Reporting Limit The Reporting Limit is the lowest number of spores that can be detected based on the total volume of the sample collected and the percentage of the slide that is counted. At Hayes Microbial, 00 the slide is read so the LOD is based solely on the total volume. spore counts that exceed 500 spores will be estimated. Blanks Results have not been corrected for field or laboratory blanks. Background The Background is the amount of debris that is present in the sample. This debris consists of skin cells, dirt, dust, pollen, drywall dust and other organic and non-organic matter. As the background density increases, the likelihood of spores, especially small spores such as those of Aspergillus and Penicillium may be obscured. The background is rated on a scale of to 4 and each level is determined as follows: 4 5 : No background detected. (Pump or cassette malfunction.) Recollect sample. : <5 field occluded. No spores will be uncountable. : 5-5 field occluded. : 5-75 field occluded. : field occluded. : >90 field occluded. Suggest recollection of sample. Fragments Fragments are small pieces of fungal mycelium or spores. They are not identifiable as to type and when present in very large numbers, may indicate the presence of mold amplification. Indoor/Outdoor Comparisons There are no national standards for the numbers of fungal spores that may be present in the indoor environment. As a general rule and guideline that is widely accepted in the indoor air quality field, the numbers and types of spores that are present in the indoor environment should not exceed those that are present outdoors at any given time. There will always be some mold spores present in "normal" indoor environments. The purpose of sampling and counting spores is to help determine whether an abnormal condition exists within the indoor environment and if it does, to help pinpoint the area of contamination. Spore counts should not be used as the sole determining factor of mold contamination. There are many factors that can cause anomalies in the comparison of indoor and outdoor samples due to the dynamic nature of both of those environments. Water Damage Indicators These molds are commonly seen in conditions of prolonged water intrusion and usually indicate a problem. Common Allergens Although all molds are potential allergens, these are the most common allergens that may be found indoors. Slightly Higher than Outside Air The spore count is slightly higher than the outside count and may or may not indicate a source of contamination. Significantly Higher than Outside Air The spore count is significantly higher than the outdoor count and probably indicates a source of contamination. Ratio Abnormality Color Note The types of spores found indoors should be similar to the ones that were identified in the outdoor sample. Significant increases (more than 5%) in the ratio of a particular spore type may indicate the presence of abnormal levels of mold, even if the total number of spores of that type is lower in the indoor environment than it was outdoors. Fungi that are present in indoor samples at levels lower than 00 per cubic meter are not color coded on the report, unless they are one of the water damage indicators. Page 6 of

28 Organism Descriptions HMC #80467 Alternaria Habitat: Commonly found outdoors in soil and decaying plants. Indoors, it is commonly found on window sills and other horizontal surfaces. Health Effects: A common allergen and has been associated with hypersensitivity pneumonitis. Alternaria is capable of producing toxic metabolites which may be associated with disease in humans or animals. Occasionally an agent of onychomycosis, ulcerated cutaneous infection and chronic sinusitis, principally in the immunocompromised patient. Ascospores Habitat: A large group consisting of more than 000 species of fungi. Common plant pathogens and outdoor numbers become very high following rain. Most of the genera are indistinguishable by spore trap analysis and are combined on the report. Health Effects: Health affects are poorly studied, but many are likely to be allergenic. Aspergillus Penicillium Habitat: The most common fungi isolated from the environment. Very common in soil and on decaying plant material. Are able to grow well indoors on a wide variety of substrates. Health Effects: This group contains common allergens and many can cause hypersensitivity pneumonitis. They may cause extrinsic asthma, and many are opportunistic pathogens. Many species produce mycotoxins which may be associated with disease in humans and other animals. Toxin production is dependent on the species, the food source, competition with other organisms, and other environmental conditions. Basidiospores Habitat: A common group of Fungi that includes the mushrooms and bracket fungi. They are saprophytes and plant pathogens. In wet conditions they can cause structural damage to buildings. Health Effects: Common allergens and are also associated with hypersensitivity pneumonitis. Cercospora Habitat: Found on wood and decaying plant matter. Health Effects: Health effects are poorly studied. Cladosporium Habitat: One of the most common genera worldwide. Found in soil and plant debris and on the leaf surfaces of living plants. The outdoor numbers are lower in the winter and often relatively high in the summer, especially in high humidity. The outdoor numbers often spike in the late afternoon and evening. Indoors, it can be found growing on textiles, wood, sheetrock, moist window sills and in HVAC supply ducts. Health Effects: A common allergen, producing more than 0 allergenic antigens and a common cause of hypersensitivity pneumonitis. Curvularia Habitat: They exist in soil and plant debris, and are plant pathogens. Health Effects: They are allergenic and a common cause of allergic fungal sinusitis. An occasional cause of human infection, including keratitis, sinusitis, onychomycosis, mycetoma, pneumonia, endocarditis and desseminated infection, primarily in the immunocompromised. Page 7 of

29 Organism Descriptions HMC #80467 Epicoccum Habitat: It is found in soil and plant litter and is a plant pathogen. It can grow indoors on a variety of substrates, including paper and textiles and is commonly found on wet drywall. Health Effects: It is a common allergen. No cases of infection have been reported in humans. Myxomycetes Habitat: Found on decaying plant material and as a plant pathogen. Health Effects: Some allergenic properties reported, but generally pose no health concerns to humans. Nigrospora Habitat: Found on wood, soil and decaying plant matter. Health Effects: Health effects are poorly studied. Pithomyces Habitat: Common fungus isolated from soil, decaying plant material. Rarely found indoors. Health Effects: Allergenic properties are poorly studied. No cases of infection in humans. Stachybotrys Habitat: Commonly found in soil and on decaying plant material. It is cellulolytic, and can be found indoors on wet materials containing cellulose, such as wallboard, ceiling tile, and other paper-based materials. It is found outdoors on decaying plant material although it is rarely detected on outdoor air samples. Health Effects: Allergenic properties are poorly studied and no cases of infection have been reported in humans. They are capable of producing potent tricothecene mycotoxins. The toxins produced by this fungus can suppress the immune system affecting the lymphoid tissue and the bone marrow. The mycotoxin is also reported to be a liver and kidney carcinogen. Torula Habitat: Found in soil and on wood and grasses. Occasionally found growing indoors on cellulose containing materials. Health Effects: A known allergen. No known cases of human infection. Page 8 of

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35 HMC # Analysis Report prepared for 5 Route Suite #C4 Brewster, NY Phone: (845) Fax: (845) Job Number: 89.0-PM Job Name: Date Sampled: Date Analyzed: Report EPA Laboratory ID# VA049 AIHA EMPAT Lab ID# 8886 Mold License: LAB0 License: #PH-098 Page of

36 HMC # Route Suite #C4 October, 08 Client Job Number: Client Job Name: 89.0-PM Dear, We would like to thank you for trusting Hayes Microbial for your analytical needs. On October, 08 we received 48 samples by FedEx for the job referenced above. 48 samples were received in good condition. The results in this analysis pertain only to this job, collected on the stated date and should not be used in the interpretation of any other job. This report may not be duplicated, except in full, without the written consent of Hayes Microbial Consulting, LLC. This laboratory bears no responsibility for sample collection activities, analytical method limitations, or your use of the test results. Interpretation and use of test results are your responsibility. Any reference to health effects or interpretation of mold levels is strictly the opinion of Hayes Microbial Consulting. In no event, shall Hayes Microbial Consulting or any of its employees be liable for lost profits or any special, incidental or consequential damages arising out of your use of the test results. Steve Hayes, BSMT(ASCP) Laboratory Director Hayes Microbial Consulting, LLC Page of

37 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium nd Fl - Room 0 spores/m /M / M Signature: HMC # Date Collected: Date Received: Date Reported: nd Fl - Storage 8 spores/m SOP #HMC0 / M nd Fl - Storage 6 spores/m / M.5% 7 8.8% 0.7%.0% % % 7.0% 4.% 07 8 Water Damage Indicator Job Name: Spore Trap Analysis 7 Common Allergen Slightly Higher than Outside Air 0/0/08 Reviewed by: 0/0/08 0/0/08 0/0/ nd Fl - Room 08 spores/m / M 0.8% 8.9%.% 9 6.7% % 7.4% Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page of

38 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium Water Damage Indicator Signature: Job Name: nd Fl - Room 06 spores/m / M 8 7.5% 7.9% 7.7%.5% SOP #HMC0 HMC # Date Collected: Date Received: Date Reported: nd Fl - Storage spores/m /M Spore Trap Analysis / M nd Fl - Comm Rm 4 spores/m / M 0/0/08 0/0/08 0/0/ nd Fl - Elec Room spores/m / M 7.0% 0.8% 7.6% % 7.5%.9% % % %.5% 8.7% 7.0% 0.8% Common Allergen Slightly Higher than Outside Air 0/0/08 Reviewed by: Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page 4 of

39 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium nd Fl - Staff Only spores/m / M Signature: % % / M Date Collected: Date Received: Date Reported: nd Fl - Staff Only 09 spores/m / M 0/0/08 0/0/08 0/0/ nd Fl - Room 0 spores/m / M 7.5% % 0.8% % % % 7.5% 7.5% Slightly Higher than Outside Air HMC # Common Allergen SOP #HMC nd Fl - Room 04 spores/m 6 0 Water Damage Indicator Job Name: Spore Trap Analysis 0/0/08 Reviewed by: Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page 5 of

40 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium Water Damage Indicator Signature: Job Name: nd Fl - Staff Bathroom 0 spores/m / M 6.5% 60 / M 80.4% % 0.%.% 6.5% % 6.5% 6.5% Slightly Higher than Outside Air 0/0/08 HMC # Date Collected: Date Received: Date Reported: nd Fl - Room 0 spores/m 6 Common Allergen SOP #HMC nd Fl - Staff Bathroom 9 spores/m Spore Trap Analysis Reviewed by: / M 0/0/08 0/0/08 0/0/ nd Fl - Room 05 spores/m / M.5% 7.6% 0 7.5% % 7.0% Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page 6 of

41 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium nd Fl - Girls Toilet spores/m / M Signature: HMC # Date Collected: Date Received: Date Reported: nd Fl - Boys Toilet spores/m SOP #HMC0 / M nd Fl - Room 07 spores/m /M / M 0/0/08 0/0/08 0/0/ nd Fl - Storage spores/m / M %.% 8.% 9.8% % % % 0.% 5.4%.% 4 5.% %.% Water Damage Indicator Job Name: Spore Trap Analysis Common Allergen Slightly Higher than Outside Air 0/0/08 Reviewed by: Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page 7 of

42 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium nd Fl - Storage spores/m / M Signature: HMC # Date Collected: Date Received: Date Reported: nd Fl - Room 09 spores/m SOP #HMC0 / M nd Fl - Guidance Suite spores/m / M 0/0/08 0/0/08 0/0/ nd Fl - Guidance Conference spores/m /M / M 7 4.4% 7 8.% 7.% 8.9% % % % 7.4%.4% % % % 7.% 5.4% Water Damage Indicator Job Name: Spore Trap Analysis 87 5 Common Allergen Slightly Higher than Outside Air 0/0/08 Reviewed by: Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page 8 of

43 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium nd Fl - Psych spores/m / M Signature:.% SOP #HMC0 HMC # Date Collected: Date Received: Date Reported: nd Fl - Guidance Office spores/m 7 Water Damage Indicator Job Name: Spore Trap Analysis / M nd Fl - SW Office spores/m / M 0/0/08 0/0/08 0/0/ nd Fl - Admin Suite spores/m /M / M %.9%.5% 6.% % 55.6% 4 8.8% 6.% %.% % %.% < % Common Allergen 6 Slightly Higher than Outside Air 0/0/08 Reviewed by: 6 Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page 9 of

44 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism nd Fl - Assist Principal spores/m / M Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium 48 4 Water Damage Indicator Signature: Job Name: SOP #HMC0 HMC # Date Collected: Date Received: Date Reported: nd Fl - Principal spores/m < % 0.9% < % 5.0% Spore Trap Analysis / M nd Fl - Nurses Office spores/m / M nd Fl - Exam Room spores/m /M / M 0 7.7% 6 0.5% 0 8.0% % % % 7 < % < % 7.6% % % < %.0% < % 5 67.% 4 5.6% Slightly Higher than Outside Air 0 60 Common Allergen 0/0/08 0/0/08 0/0/08 0/0/08 Reviewed by: Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page 0 of

45 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium Water Damage Indicator Signature: Job Name: nd Fl - Nurses Room 04B spores/m / M SOP #HMC0 HMC # Date Collected: Date Received: Date Reported: nd Fl - Nurses Bathroom spores/m Spore Trap Analysis / M nd Fl - Parent Center spores/m / M 0/0/08 0/0/08 0/0/ nd Fl - Auditorium Lobby spores/m /M / M % % 9.4% 4.% % % % 6 76.% < % % 7 9.6% < % < % Common Allergen 907 Slightly Higher than Outside Air 0/0/08 Reviewed by: Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page of

46 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium nd Fl - Room 0A Coats spores/m / M Signature: HMC # nd Fl - Auditorium Back spores/m SOP #HMC0 / M Date Collected: Date Received: Date Reported: nd Fl - Auditorium Front spores/m / M 0/0/08 0/0/08 0/0/ nd Fl - Stage spores/m / M % 4.6% 57.% 4.0% % 6.% 7 9.0% % % 4.% % % 8.8% 4.4% 9 6 Water Damage Indicator Job Name: Spore Trap Analysis 80 7 Common Allergen 9 Slightly Higher than Outside Air 0/0/08 Reviewed by: Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page of

47 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium nd Fl - Hallway North spores/m / M Signature: HMC # Date Collected: Date Received: Date Reported: nd Fl - Hallway Center spores/m SOP #HMC0 / M nd Fl - Hallway South spores/m / M 07 0.% 5.0% % % 6 80.% %.4% 7 5.5% 0 Common Allergen 66 Slightly Higher than Outside Air 0/0/ < % 8.0% < % 54.% < % < % % < % 60.4% < % / M 4.9%.4% 5 0/0/08 0/0/08 0/0/ Exterior North spores/m /M 8 Water Damage Indicator Job Name: Spore Trap Analysis Reviewed by: Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page of

48 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Cercospora Chaetomium Cladosporium Curvularia Epicoccum Memnoniella Myxomycetes Nigrospora Pithomyces Stachybotrys Stemphylium Torula Ulocladium Exterior - East spores/m /M / M Signature: / M % < % 55.% < % % < % < % < % < % 0 89 Common Allergen Exterior West spores/m 5/M < % 8.9% < % 57.9% / M % < % 7.9% < %.6% % < % < % < % 5 67 < % < % < % /0/08 Reviewed by: 0/0/08 0/0/08 0/0/ Field Blank 0 liters 0 spores/m Slightly Higher than Outside Air HMC # Date Collected: Date Received: Date Reported: Exterior South spores/m /M SOP #HMC Water Damage Indicator Job Name: Spore Trap Analysis Significantly Higher than Outside Air / M Ratio Abnormality 0/0/08 Page 4 of

49 Spore Trap Information HMC # Reporting Limit The Reporting Limit is the lowest number of spores that can be detected based on the total volume of the sample collected and the percentage of the slide that is counted. At Hayes Microbial, 00 the slide is read so the LOD is based solely on the total volume. spore counts that exceed 500 spores will be estimated. Blanks Results have not been corrected for field or laboratory blanks. Background The Background is the amount of debris that is present in the sample. This debris consists of skin cells, dirt, dust, pollen, drywall dust and other organic and non-organic matter. As the background density increases, the likelihood of spores, especially small spores such as those of Aspergillus and Penicillium may be obscured. The background is rated on a scale of to 4 and each level is determined as follows: 4 5 : No background detected. (Pump or cassette malfunction.) Recollect sample. : <5 field occluded. No spores will be uncountable. : 5-5 field occluded. : 5-75 field occluded. : field occluded. : >90 field occluded. Suggest recollection of sample. Fragments Fragments are small pieces of fungal mycelium or spores. They are not identifiable as to type and when present in very large numbers, may indicate the presence of mold amplification. Indoor/Outdoor Comparisons There are no national standards for the numbers of fungal spores that may be present in the indoor environment. As a general rule and guideline that is widely accepted in the indoor air quality field, the numbers and types of spores that are present in the indoor environment should not exceed those that are present outdoors at any given time. There will always be some mold spores present in "normal" indoor environments. The purpose of sampling and counting spores is to help determine whether an abnormal condition exists within the indoor environment and if it does, to help pinpoint the area of contamination. Spore counts should not be used as the sole determining factor of mold contamination. There are many factors that can cause anomalies in the comparison of indoor and outdoor samples due to the dynamic nature of both of those environments. Water Damage Indicators These molds are commonly seen in conditions of prolonged water intrusion and usually indicate a problem. Common Allergens Although all molds are potential allergens, these are the most common allergens that may be found indoors. Slightly Higher than Outside Air The spore count is slightly higher than the outside count and may or may not indicate a source of contamination. Significantly Higher than Outside Air The spore count is significantly higher than the outdoor count and probably indicates a source of contamination. Ratio Abnormality Color Note The types of spores found indoors should be similar to the ones that were identified in the outdoor sample. Significant increases (more than 5%) in the ratio of a particular spore type may indicate the presence of abnormal levels of mold, even if the total number of spores of that type is lower in the indoor environment than it was outdoors. Fungi that are present in indoor samples at levels lower than 00 per cubic meter are not color coded on the report, unless they are one of the water damage indicators. Page 5 of

50 Organism Descriptions HMC # Alternaria Habitat: Commonly found outdoors in soil and decaying plants. Indoors, it is commonly found on window sills and other horizontal surfaces. Health Effects: A common allergen and has been associated with hypersensitivity pneumonitis. Alternaria is capable of producing toxic metabolites which may be associated with disease in humans or animals. Occasionally an agent of onychomycosis, ulcerated cutaneous infection and chronic sinusitis, principally in the immunocompromised patient. Ascospores Habitat: A large group consisting of more than 000 species of fungi. Common plant pathogens and outdoor numbers become very high following rain. Most of the genera are indistinguishable by spore trap analysis and are combined on the report. Health Effects: Health affects are poorly studied, but many are likely to be allergenic. Aspergillus Penicillium Habitat: The most common fungi isolated from the environment. Very common in soil and on decaying plant material. Are able to grow well indoors on a wide variety of substrates. Health Effects: This group contains common allergens and many can cause hypersensitivity pneumonitis. They may cause extrinsic asthma, and many are opportunistic pathogens. Many species produce mycotoxins which may be associated with disease in humans and other animals. Toxin production is dependent on the species, the food source, competition with other organisms, and other environmental conditions. Basidiospores Habitat: A common group of Fungi that includes the mushrooms and bracket fungi. They are saprophytes and plant pathogens. In wet conditions they can cause structural damage to buildings. Health Effects: Common allergens and are also associated with hypersensitivity pneumonitis. Bipolaris Drechslera Habitat: They are found in soil and as plant pathogens. Can grow indoors on a variety of substrates. Health Effects: They may be allergenic and are very commonly involved in allergic fungal sinusitis. They are opportunistic pathogens but occasionally infect healthy individuals, causing keratitis, sinusitis and osteomyelitis. Cercospora Habitat: Found on wood and decaying plant matter. Health Effects: Health effects are poorly studied. Cladosporium Habitat: One of the most common genera worldwide. Found in soil and plant debris and on the leaf surfaces of living plants. The outdoor numbers are lower in the winter and often relatively high in the summer, especially in high humidity. The outdoor numbers often spike in the late afternoon and evening. Indoors, it can be found growing on textiles, wood, sheetrock, moist window sills and in HVAC supply ducts. Health Effects: A common allergen, producing more than 0 allergenic antigens and a common cause of hypersensitivity pneumonitis. Page 6 of

51 Organism Descriptions HMC # Curvularia Habitat: They exist in soil and plant debris, and are plant pathogens. Health Effects: They are allergenic and a common cause of allergic fungal sinusitis. An occasional cause of human infection, including keratitis, sinusitis, onychomycosis, mycetoma, pneumonia, endocarditis and desseminated infection, primarily in the immunocompromised. Epicoccum Habitat: It is found in soil and plant litter and is a plant pathogen. It can grow indoors on a variety of substrates, including paper and textiles and is commonly found on wet drywall. Health Effects: It is a common allergen. No cases of infection have been reported in humans. Myxomycetes Habitat: Found on decaying plant material and as a plant pathogen. Health Effects: Some allergenic properties reported, but generally pose no health concerns to humans. Nigrospora Habitat: Found on wood, soil and decaying plant matter. Health Effects: Health effects are poorly studied. Pithomyces Habitat: Common fungus isolated from soil, decaying plant material. Rarely found indoors. Health Effects: Allergenic properties are poorly studied. No cases of infection in humans. Stachybotrys Habitat: Commonly found in soil and on decaying plant material. It is cellulolytic, and can be found indoors on wet materials containing cellulose, such as wallboard, ceiling tile, and other paper-based materials. It is found outdoors on decaying plant material although it is rarely detected on outdoor air samples. Health Effects: Allergenic properties are poorly studied and no cases of infection have been reported in humans. They are capable of producing potent tricothecene mycotoxins. The toxins produced by this fungus can suppress the immune system affecting the lymphoid tissue and the bone marrow. The mycotoxin is also reported to be a liver and kidney carcinogen. Torula Habitat: Found in soil and on wood and grasses. Occasionally found growing indoors on cellulose containing materials. Health Effects: A known allergen. No known cases of human infection. Page 7 of

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56 HMC # Analysis Report prepared for 5 Route Suite #C4 Brewster, NY Phone: (845) Fax: (845) Job Number: 89.0-PM Job Name: Date Sampled: Date Analyzed: Report EPA Laboratory ID# VA049 AIHA EMPAT Lab ID# 8886 Mold License: LAB0 License: #PH-098 Page of 7

57 HMC # Route Suite #C4 October, 08 Client Job Number: Client Job Name: 89.0-PM Dear, We would like to thank you for trusting Hayes Microbial for your analytical needs. On October, 08 we received 5 samples by FedEx for the job referenced above. 5 samples were received in good condition. The results in this analysis pertain only to this job, collected on the stated date and should not be used in the interpretation of any other job. This report may not be duplicated, except in full, without the written consent of Hayes Microbial Consulting, LLC. This laboratory bears no responsibility for sample collection activities, analytical method limitations, or your use of the test results. Interpretation and use of test results are your responsibility. Any reference to health effects or interpretation of mold levels is strictly the opinion of Hayes Microbial Consulting. In no event, shall Hayes Microbial Consulting or any of its employees be liable for lost profits or any special, incidental or consequential damages arising out of your use of the test results. Steve Hayes, BSMT(ASCP) Laboratory Director Hayes Microbial Consulting, LLC Page of 7

58 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Chaetomium Cladosporium Curvularia Epicoccum Fusarium Memnoniella Myxomycetes Pithomyces Stachybotrys Stemphylium Torula Ulocladium Unspecified Spore Water Damage Indicator Signature: Job Name: rd Fl - Room 0A spores/m / M % 8.% 4.0% 7 8.% 5 4 / M 4 8 Common Allergen 0/0/08 HMC # Date Collected: Date Received: Date Reported: rd Fl - Room 0 spores/m /M 7.4% 5.4%.% 9 Slightly Higher than Outside Air SOP #HMC rd Fl - Rom 0 spores/m Spore Trap Analysis Reviewed by: / M 0/0/08 0/0/08 0/0/ rd Fl - Staff Restroom spores/m %.6% 5 67.% 6 4 Significantly Higher than Outside Air / M % 4.0% Ratio Abnormality 0/0/08 Page of 7

59 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Chaetomium Cladosporium Curvularia Epicoccum Fusarium Memnoniella Myxomycetes Pithomyces Stachybotrys Stemphylium Torula Ulocladium Unspecified Spore rd Fl - Staff Restroom spores/m / M Signature: HMC # Date Collected: Date Received: Date Reported: rd Fl - Custodial Closet spores/m /M SOP #HMC0 / M rd Fl - Room 05 spores/m 7/M / M % 0.% % 9.7% 9.% % 7.% 5.% 9.8% % 9 7.9%.4% 69 Common Allergen 44 Slightly Higher than Outside Air 0/0/08 Reviewed by: 0/0/08 0/0/08 0/0/ rd Fl - Girls Bathroom spores/m 7 0 Water Damage Indicator Job Name: Spore Trap Analysis Significantly Higher than Outside Air / M % 9.8% 0.% Ratio Abnormality 0/0/08 Page 4 of 7

60 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Chaetomium Cladosporium Curvularia Epicoccum Fusarium Memnoniella Myxomycetes Pithomyces Stachybotrys Stemphylium Torula Ulocladium Unspecified Spore rd Fl - Boys Bathroom spores/m /M / M Signature: HMC # Date Collected: Date Received: Date Reported: rd Fl - Room 07 spores/m SOP #HMC0 / M rd Fl - Room 09 spores/m / M 0/0/08 0/0/08 0/0/ rd Fl - Room 0 spores/m / M % 7.0% 4.4% 47 6.% 6.7% 5.4% % 9.5%.8% % 5.8% 7.7% 7 4.4% 7.% 7 4.8%.% 5.8% 5.4% 7 4.8% Water Damage Indicator Job Name: Spore Trap Analysis 87 Common Allergen Slightly Higher than Outside Air 0/0/08 Reviewed by: Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page 5 of 7

61 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Chaetomium Cladosporium Curvularia Epicoccum Fusarium Memnoniella Myxomycetes Pithomyces Stachybotrys Stemphylium Torula Ulocladium Unspecified Spore Water Damage Indicator Signature: Job Name: rd Fl - Stray Room spores/m / M % 7 8.4% 47 / M % 8.6%.4% % 8.8% Common Allergen 466 Slightly Higher than Outside Air 0/0/08 HMC # Date Collected: Date Received: Date Reported: rd Fl - Room 06 spores/m /M SOP #HMC rd Fl - Room 08 spores/m /M Spore Trap Analysis Reviewed by: / M 0/0/08 0/0/08 0/0/ rd Fl - Mech Room spores/m /M / M % 5.8%.5% %.6%.6% 7.0% 7.9% 4.4%.6% Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page 6 of 7

62 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Chaetomium Cladosporium Curvularia Epicoccum Fusarium Memnoniella Myxomycetes Pithomyces Stachybotrys Stemphylium Torula Ulocladium Unspecified Spore rd Fl - Comm Room spores/m / M Signature: HMC # Date Collected: Date Received: Date Reported: rd Fl - Elec Room spores/m 7/M SOP #HMC0 / M rd Fl - Storage 06 spores/m /M / M % 7.5%.5% % 4.8% 7.6% % 5.% 0.5%.4% % < % 7 4.% %.6% Common Allergen Slightly Higher than Outside Air 0/0/08 Reviewed by: 0/0/08 0/0/08 0/0/ rd Fl - Room 04 spores/m 7 9 Water Damage Indicator Job Name: Spore Trap Analysis Significantly Higher than Outside Air / M % 4.% 6.% Ratio Abnormality 0/0/08 Page 7 of 7

63 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Chaetomium Cladosporium Curvularia Epicoccum Fusarium Memnoniella Myxomycetes Pithomyces Stachybotrys Stemphylium Torula Ulocladium Unspecified Spore rd Fl - Room 0 spores/m / M Signature: HMC # Date Collected: Date Received: Date Reported: rd Fl - ICT 4 spores/m /M SOP #HMC0 / M rd Fl - ICT spores/m / M % 8.% 4.5% %.% 0.0% % % %.% Common Allergen Slightly Higher than Outside Air 0/0/08 Reviewed by: 0 0/0/08 0/0/08 0/0/ rd Fl - Library spores/m Water Damage Indicator Job Name: Spore Trap Analysis / M 0.% % 9.% 8.% 9.8% 4.4% 7 9.% 9 Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page 8 of 7

64 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Chaetomium Cladosporium Curvularia Epicoccum Fusarium Memnoniella Myxomycetes Pithomyces Stachybotrys Stemphylium Torula Ulocladium Unspecified Spore rd Fl - Work Room spores/m / M Signature: 8.% 7 6.9% 60 Common Allergen HMC # Date Collected: Date Received: Date Reported: / M rd Fl - Staff Lounge spores/m / M 0/0/08 0/0/08 0/0/ rd Fl - Hallway North spores/m / M % 8.9% 8.9% % 6.5%.5% %.5%.4% 7 8.5% 6.5% 7 7.% Slightly Higher than Outside Air SOP #HMC rd Fl - Reading Resource spores/m 0 Water Damage Indicator Job Name: Spore Trap Analysis 0/0/08 Reviewed by: Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page 9 of 7

65 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Job Name: rd Fl - Hallway Center spores/m /M Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Chaetomium Cladosporium Curvularia Epicoccum Fusarium Memnoniella Myxomycetes Pithomyces Stachybotrys Stemphylium Torula Ulocladium Unspecified Spore % % Water Damage Indicator Signature: / M Common Allergen / M % 7.5% 9 0 0/0/08 HMC # Date Collected: Date Received: Date Reported: Exterior North spores/m /M 0.8% Slightly Higher than Outside Air SOP #HMC rd Fl - Hallway South spores/m.0% 5.6% 6.% 9.5% Organism Spore Trap Analysis Reviewed by: Exterior East spores/m 7/M < % 49.8% 4.7% 8.4% / M 0/0/08 0/0/08 0/0/ < % 47.7%.6% 4.9% 8.7% < % % 7.4% < % 80 4.% < % Significantly Higher than Outside Air / M Ratio Abnormality 0/0/08 Page 0 of 7

66 Job Number: Collected by: PM John Soter HMC ID Number Sample ID# Sample Name Sample Volume Reporting Limit Background Fragments Organism Alternaria Ascospores Aspergillus Penicillium Basidiospores Bipolaris Drechslera Chaetomium Cladosporium Curvularia Epicoccum Fusarium Memnoniella Myxomycetes Pithomyces Stachybotrys Stemphylium Torula Ulocladium Unspecified Spore Water Damage Indicator Signature: Job Name: Exterior South spores/m 5/M Exterior West spores/m /M % 5.% 0.0% % < % % < % 4 5.5% < % % < % 9 0.6% / M Slightly Higher than Outside Air 0/0/08 HMC # Date Collected: Date Received: Date Reported: 0/0/08 0/0/08 0/0/ Field Blank 0 liters 0 spores/m Common Allergen SOP #HMC0 < % 5.4% 6.4% 4.7% / M Spore Trap Analysis Reviewed by: / M Significantly Higher than Outside Air Ratio Abnormality 0/0/08 Page of 7

67 Spore Trap Information HMC # Reporting Limit The Reporting Limit is the lowest number of spores that can be detected based on the total volume of the sample collected and the percentage of the slide that is counted. At Hayes Microbial, 00 the slide is read so the LOD is based solely on the total volume. spore counts that exceed 500 spores will be estimated. Blanks Results have not been corrected for field or laboratory blanks. Background The Background is the amount of debris that is present in the sample. This debris consists of skin cells, dirt, dust, pollen, drywall dust and other organic and non-organic matter. As the background density increases, the likelihood of spores, especially small spores such as those of Aspergillus and Penicillium may be obscured. The background is rated on a scale of to 4 and each level is determined as follows: 4 5 : No background detected. (Pump or cassette malfunction.) Recollect sample. : <5 field occluded. No spores will be uncountable. : 5-5 field occluded. : 5-75 field occluded. : field occluded. : >90 field occluded. Suggest recollection of sample. Fragments Fragments are small pieces of fungal mycelium or spores. They are not identifiable as to type and when present in very large numbers, may indicate the presence of mold amplification. Indoor/Outdoor Comparisons There are no national standards for the numbers of fungal spores that may be present in the indoor environment. As a general rule and guideline that is widely accepted in the indoor air quality field, the numbers and types of spores that are present in the indoor environment should not exceed those that are present outdoors at any given time. There will always be some mold spores present in "normal" indoor environments. The purpose of sampling and counting spores is to help determine whether an abnormal condition exists within the indoor environment and if it does, to help pinpoint the area of contamination. Spore counts should not be used as the sole determining factor of mold contamination. There are many factors that can cause anomalies in the comparison of indoor and outdoor samples due to the dynamic nature of both of those environments. Water Damage Indicators These molds are commonly seen in conditions of prolonged water intrusion and usually indicate a problem. Common Allergens Although all molds are potential allergens, these are the most common allergens that may be found indoors. Slightly Higher than Outside Air The spore count is slightly higher than the outside count and may or may not indicate a source of contamination. Significantly Higher than Outside Air The spore count is significantly higher than the outdoor count and probably indicates a source of contamination. Ratio Abnormality Color Note The types of spores found indoors should be similar to the ones that were identified in the outdoor sample. Significant increases (more than 5%) in the ratio of a particular spore type may indicate the presence of abnormal levels of mold, even if the total number of spores of that type is lower in the indoor environment than it was outdoors. Fungi that are present in indoor samples at levels lower than 00 per cubic meter are not color coded on the report, unless they are one of the water damage indicators. Page of 7

68 Organism Descriptions HMC # Alternaria Habitat: Commonly found outdoors in soil and decaying plants. Indoors, it is commonly found on window sills and other horizontal surfaces. Health Effects: A common allergen and has been associated with hypersensitivity pneumonitis. Alternaria is capable of producing toxic metabolites which may be associated with disease in humans or animals. Occasionally an agent of onychomycosis, ulcerated cutaneous infection and chronic sinusitis, principally in the immunocompromised patient. Ascospores Habitat: A large group consisting of more than 000 species of fungi. Common plant pathogens and outdoor numbers become very high following rain. Most of the genera are indistinguishable by spore trap analysis and are combined on the report. Health Effects: Health affects are poorly studied, but many are likely to be allergenic. Aspergillus Penicillium Habitat: The most common fungi isolated from the environment. Very common in soil and on decaying plant material. Are able to grow well indoors on a wide variety of substrates. Health Effects: This group contains common allergens and many can cause hypersensitivity pneumonitis. They may cause extrinsic asthma, and many are opportunistic pathogens. Many species produce mycotoxins which may be associated with disease in humans and other animals. Toxin production is dependent on the species, the food source, competition with other organisms, and other environmental conditions. Basidiospores Habitat: A common group of Fungi that includes the mushrooms and bracket fungi. They are saprophytes and plant pathogens. In wet conditions they can cause structural damage to buildings. Health Effects: Common allergens and are also associated with hypersensitivity pneumonitis. Cladosporium Habitat: One of the most common genera worldwide. Found in soil and plant debris and on the leaf surfaces of living plants. The outdoor numbers are lower in the winter and often relatively high in the summer, especially in high humidity. The outdoor numbers often spike in the late afternoon and evening. Indoors, it can be found growing on textiles, wood, sheetrock, moist window sills and in HVAC supply ducts. Health Effects: A common allergen, producing more than 0 allergenic antigens and a common cause of hypersensitivity pneumonitis. Curvularia Habitat: They exist in soil and plant debris, and are plant pathogens. Health Effects: They are allergenic and a common cause of allergic fungal sinusitis. An occasional cause of human infection, including keratitis, sinusitis, onychomycosis, mycetoma, pneumonia, endocarditis and desseminated infection, primarily in the immunocompromised. Page of 7

69 Organism Descriptions HMC # Myxomycetes Habitat: Found on decaying plant material and as a plant pathogen. Health Effects: Some allergenic properties reported, but generally pose no health concerns to humans. Pithomyces Habitat: Common fungus isolated from soil, decaying plant material. Rarely found indoors. Health Effects: Allergenic properties are poorly studied. No cases of infection in humans. Torula Habitat: Found in soil and on wood and grasses. Occasionally found growing indoors on cellulose containing materials. Health Effects: A known allergen. No known cases of human infection. Page 4 of 7

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73 APPEIX C PERSONNEL A LABORATORY CERTIFICATIONS

74 NEW YORK STATE - DEPARTMENT OF LABOR DIVISION OF SAFETY A HEALTH LICENSE A CERTIFICATE UNIT STATE CAMPUS BUILDING Mold Assessor Company License Adelaide Env Health Assoc,Inc 5 Rte Suite C4 BREWSTER, NY 0509 LICENSE NUMBER DATE OF ISSUE: //08 EXPIRATION DATE //09 This license is valid only for the contractor named above. Eileen Franko, Director FOR THE COMMISSIONER OF LABOR

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76 September 8, 08 Laboratory ID: 8886 Stephen Hayes Hayes Microbial Consulting 005 E. Boundary Terrace, Suite F Midlothian, VA Dear Mr. Hayes: Congratulations! The AIHA Laboratory Accreditation Programs (AIHA-LAP), LLC s Analytical Accreditation Board (AAB) has approved Hayes Microbial Consulting as an accredited Environmental Microbiology laboratory. Accreditation documentation includes the EMLAP accreditation certificate, scope of accreditation document and a copy of the current AIHA-LAP, LLC license agreement (if your completed agreement is not on file at AIHA-LAP, LLC). The accreditation symbol has been designed for use by all AIHA-LAP, LLC accredited laboratories. If your laboratory chooses to use the symbol in its advertising the laboratory s accreditation, you must complete and return the AIHA-LAP, LLC license agreement to a Laboratory Accreditation Specialist. Once submitted, an electronic copy of the accreditation symbol will be sent to you. Laboratory accreditation shall be maintained by continued compliance with EMLAP requirements (see Policy Modules D and 6), which includes proficient participation in AIHA-LAP, LLC approved proficiency testing, demonstration of competency, or round robin program as indicated on the AIHA-LAP Approved PT and Round Robin webpage, its associated Scope/PT table, and as required in Policy Module 6, for all Fields of Testing (FoTs) for which the laboratory is accredited. An accredited laboratory that wishes to expand into a new FoT must submit an updated accreditation application to AIHA-LAP, LLC for review by the AAB. Any changes in ownership, laboratory location, personnel, FoTs/Methods, or significant procedural changes shall be reported to AIHA-LAP, LLC in writing within twenty (0) business days of the change. The accreditation certificate is the property of AIHA-LAP, LLC and must be returned to us should your laboratory withdraw or be removed from the EMLAP. Again, congratulations. If you have any questions, please contact Lauren Schnack, Laboratory Accreditation Specialist, at (70) Sincerely, Cheryl O. Morton Managing Director AIHA Laboratory Accreditation Programs, LLC 4 Fairview Park Drive, Suite 777, Falls Church, VA 04 USA main fax R4 0/4/08 Page of

77 AIHA Laboratory Accreditation Programs, LLC acknowledges that Hayes Microbial Consulting 005 E. Boundary Terrace, Suite F, Midlothian, VA Laboratory ID: 8886 along with all premises from which key activities are performed, as listed above, has fulfilled the requirements of the AIHA Laboratory Accreditation Programs (AIHA-LAP), LLC accreditation to the ISO/IEC 705:005 international standard, General Requirements for the Competence of Testing and Calibration Laboratories in the following: LABORATORY ACCREDITATION PROGRAMS IUSTRIAL HYGIENE Accreditation Expires: ENVIRONMENTAL LEAD Accreditation Expires: ENVIRONMENTAL MICROBIOLOGY Accreditation Expires: August 0, 00 FOOD Accreditation Expires: UNIQUE SCOPES Accreditation Expires: Specific Field(s) of Testing (FoT)/Method(s) within each Accreditation Program for which the above named laboratory maintains accreditation is outlined on the attached Scope of Accreditation. Continued accreditation is contingent upon successful on-going compliance with ISO/IEC 705:005 and AIHA-LAP, LLC requirements. This certificate is not valid without the attached Scope of Accreditation. Please review the AIHA-LAP, LLC website ( for the most current Scope. Elizabeth Bair Chairperson, Analytical Accreditation Board Cheryl O. Morton Managing Director, AIHA Laboratory Accreditation Programs, LLC Revision 7 09//08 Date Issued: 09/8/08

78 AIHA Laboratory Accreditation Programs, LLC SCOPE OF ACCREDITATION Hayes Microbial Consulting Laboratory ID: E. Boundary Terrace, Suite F, Midlothian, VA Issue 09/8/08 The laboratory is approved for those specific field(s) of testing/methods listed in the table below. Clients are urged to verify the laboratory s current accreditation status for the particular field(s) of testing/methods, since these can change due to proficiency status, suspension and/or withdrawal of accreditation. Environmental Microbiology Laboratory Accreditation Program (EMLAP) Initial Accreditation 08/0/00 EMLAP Category Field of Testing (FoT) Method Method Description (for internal methods only) Air - Culturable HMC-#0 In-house: Analysis of Culturable Air Bulk - Culturable HMC-#04 In-house: Analysis of Culturable Bulk Fungal Surface - Culturable HMC-#05 In-house: Analysis of Culturable Swab Air - Direct Examination Bulk - Direct Examination Surface - Direct Examination HMC-#0 HMC-#0 HMC-#0 In-house: Analysis of Spore Trap In-house: Analysis of Direct Samples In-house: Analysis of Direct Samples A complete listing of currently accredited Environmental Microbiology laboratories is available on the AIHA-LAP, LLC website at: Effective: 0//0 8886_Scope_EMLAP_08_09_8 Page of