AIRBORNE FUNGAL STRUCTURE SAMPLE ANALYSIS Results reported as Structures per Cubic Meter of Air (structures/m 3 )

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1 Field #: TM Project #: Description: Background, Outside, Main Lab #: Entrance Eagle #: E Results reported as per Cubic Meter of Air Structure Identification ascospores (undifferentiated) basidiospores (undifferentiated) smuts/myxomycetes/periconia TOTAL Background Debris (0-5) 2 Page 1 of 10

2 Field #: TM Project #: Description: First Floor, Hallway at 118 Lab #: Eagle #: E Results reported as per Cubic Meter of Air Structure Identification ascospores (undifferentiated) ND 20 <34 basidiospores (undifferentiated) TOTAL Background Debris (0-5) 3 Page 2 of 10

3 Field #: TM Project #: Description: Second Floor, Hallway at Lab #: B Eagle #: E Results reported as per Cubic Meter of Air Structure Identification ascospores (undifferentiated) basidiospores (undifferentiated) Cladosporium TOTAL Background Debris (0-5) 3 Page 3 of 10

4 Field #: TM Project #: Description: First Floor, Hallway at 145 Lab #: Eagle #: E Results reported as per Cubic Meter of Air Structure Identification ascospores (undifferentiated) basidiospores (undifferentiated) ND 20 <34 TOTAL Background Debris (0-5) 3 Page 4 of 10

5 Field #: TM Project #: Description: First Floor, Hallway at 134 Lab #: Eagle #: E Results reported as per Cubic Meter of Air Structure Identification ascospores (undifferentiated) basidiospores (undifferentiated) ND 20 <34 TOTAL 1 34 Background Debris (0-5) 2 Page 5 of 10

6 Field #: TM Project #: Description: Background, Outside, South Lab #: Wing Entrance nearest Café Eagle #: E C Results reported as per Cubic Meter of Air Structure Identification ascospores (undifferentiated) basidiospores (undifferentiated) Cladosporium TOTAL Background Debris (0-5) 3 Page 6 of 10

7 Field #: TM Project #: Description: Second Floor Hallway at Lab #: Eagle #: E Results reported as per Cubic Meter of Air Structure Identification ascospores (undifferentiated) basidiospores (undifferentiated) ND 20 <34 TOTAL 1 34 Background Debris (0-5) 2 Page 7 of 10

8 Field #: TM Project #: Description: Second Floor Hallway at Lab #: Eagle #: E Results reported as per Cubic Meter of Air Structure Identification ascospores (undifferentiated) basidiospores (undifferentiated) ND 20 <34 TOTAL Background Debris (0-5) 2 Page 8 of 10

9 ASTM D "Standard Test Method for Categorization and Quantification of Airborne in an Inertial Impaction by Optical Microscopy" was used for the analysis of these samples. 20% of the sample surface (26 traverses) was viewed to count the fungal structures in the sample, unless otherwise noted. Per method, when 100 counts for a given spore type or structure is reached, the number of traverses (percent of sample surface) viewed to that point is noted, and these values are used to calculate the concentration for the given spore or structure type. This stoppage rule does not apply to water related spores- all 26 traverses are counted. The structure concentration calculation uses the percentage of the sample trace (number of traverses) viewed to count each spore or structure type and the sample air volume. The calculated structure concentrations are reported to two significant figures. The total structure concentration may not equal the sum of the individual results due to the rounding of the individual values. The detection limit for the area of the sample which was viewed is one fungal spore or structure. 400X magnification was used to view the sample, unless otherwise noted. A background debris rating of 2 to 4 indicates particulate matter may obscure a portion of the sample surface and result in underreporting of fungal structures. A background debris rating of 5 indicates the sample is overloaded with particulate matter and the fungal structures cannot be counted. A new sample should be collected at a shorter time interval, or other measures should be taken to reduce the particle load. The analysis results are not blank corrected, unless otherwise noted. Air-O-Cell slit impactor cassettes were used to collect the samples, unless otherwise stated. See the chain of custody document for sample times or volumes, the date(s) of sampling, and the date of receipt of the samples by the laboratory. The samples were received in acceptable condition unless otherwise noted on the individual sample page. The analysis relates only to the samples tested, and the "scope" of this report pertains only to those items tested and listed as such on the individual pages of this document. This report may not be reproduced in whole or in part without the expressed written consent from Eagle Industrial Hygiene Associates, Incorporated. Receipt Date: 04/28/2015 Analyst: Renee Battiste Analysis Date: 04/29/2015 Reviewed by: Don Schill, Laboratory Director Report Date: 04/29/2015 Page 9 of 10

10 Dresher Dresher Road Road Horsham, PA PA (215) Fax (215) Eagle Industrial Hygiene Associates, Inc. Structure Library (Health Effects) Predominately Outside Alternaria : considered an allergen, involved in Type I allergies and Type III hypersensitivity ascospores: majority are non-disease causing; usually occurring in high humidity or wet conditions basidiospores: may produce toxins, involved in Type I allergies and Type III hypersensitivity Bipolaris/Drechslera/Helminthosporium/Exserohilum : causes Type I allergic reactions and a common cause of allergic fungal sinusitis Curvularia : causes Type I allergic reactions and a common cause of allergic fungal sinusitis Epicoccum : a common Type I allergen Myxomycetes : known to cause Type I allergies Pithomyces : not known to be allergenic or pathogenic Torula : known to be involved in Type I allergies Inside/Outside Cladosporium : a common and important allergen, involved in Type I allergies and Type III hypersensitivity Penicillium/Aspergillus : common allergens, involved in Type I allergies and Type III hypersensitivity Paecilomyces : considered an allergen, involved in Type I allergies and Type III hypersensitivity Botrytis : considered an allergen, involved in Type I allergies and Type III hypersensitivity Water Damage Chaetomium : known to cause Type I allergies and hypersensitivity Stachybotrys : a producer of mycotoxin, involved in Type I allergies and hypersensitivity Ulocladium : a major allergen, involved in Type I allergies NOTES: Type I hypersensitivity: is an allergic reaction provoked by re-exposure to an allergen; examples are allergies and hay fever. Type II hypersensitivity: involves autoimmune diseases; examples are rheumatic fever and autoimmune hemolytic anemia. Type III hypersensitivity: involves the immune system giving an inflammatory response in the body; examples are respiratory pneumonitis and Farmer's Lung. LINK: Page 10 of 10

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