Human Amniotic Membrane. KFAFH-Jeddah. KSA. Human Amniotic Membrane. Human Amniotic Membrane 3/12/2014
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1 بسم هللا الرحمن الرحيم KFAFH-Jeddah. KSA The role of in acute and chronic wounds Tauqeer Ahmad Malik MRCS-Ed Associate Consultant Diabetic Foot & Wound Care 5 March 2014 International spinal cord injury SBAHC- Riyadh 1910 Davis used fetal membranes as a skin substitute It has been a popular Biological dressing for Burns Acute and chronic wounds Chronic leg ulcer including vascular Diabetic foot 1
2 In Head, Abdominal, Pelvic and Cardiac surgery for prevention of tissue adhesions In Dental surgery for dental sockets In Gynecology for reconstruction of neo vagina In Neurosurgery for dura repair In Ophthalmology for variety of cases HAM-Histology Human amniotic membrane or Amnion is the upper most non adherent Ectodermal layer of placenta that is facing the fetus HAM-Histology It has a single layer of Cuboidal Epithelial Cells, Thick Basement Membrane and Avascular Stromal Tissue that is loosely attached to Chorion It has certain specific characteristics : Non Immunological reaction due to lack of HLA-A, HLA-B, HLA-C and DR antigens It also has cell migration and growth promoting activity like Cytokines, Growth factors, Protease inhibitors Preserved at C for up to 2 years It has an antimicrobial activity 2
3 Protocols- General Seronegative donors & recipient HBV, HCV, HIV 1, HIV 2, Syphilis & HTLV Consent from Donors and Recipients Harvesting techniques Cleaning & preparation methods Blood & Rh Group Patient selection criteria Dressing protocols Follow-up Results Study Two Centers King Fahad Armed Forces Hospital Jeddah King Fahad General Hospital (MOH) Jeddah We have done 68 cases Diabetic Foot 35 Crush Injuries 07 Burn 14 Pressure Ulcer 08 Post operative 04 Cases 3
4 Case 1 Case 2 Week Follow Week up at 64 0 months Case 1: 59 year old male Diabetic, Hypertensive, Hyperlipidemic, PVD post right Femoropopliteal bypass,ankle Brachial Index on Right 0.9 and Left 0.8 Right 5 th toe amputation. Non healing wound at lateral dorsal foot X ray foot: no bone infection. Wound culture: negative We applied HAM Week At Week one 012 year 818 Week Week Case 2: 64 year old male Diabetic, Hypertensive, Hyperlipidemic Wound at dorsum of right foot since 2 years Ankle Brachial Index on Right 0.9 and Left 1 Extensive debridement +/- amputation was suggested in outside hospitals After initial wound management we offered him HAM Case 3 Case 4 Week Week 38 0 Case 3: 59 year old male Diabetic, Hyperlipidemic, Hypertensive. Infected wound at left 5 th toe since 1 week Ankle Brachial Index on Right 0.9 and Left 1. X rays no bony involvement. Culture no significant growth. Debridement was done After initial wound management we offered him HAM Week Week Week Week Case 4: 45 year old male Diabetic, Hypertensive. Wound at dorsum of right foot since 4 weeks. Ankle Brachial Index on Right 0.9 and Left 1 X rays no bony involvement. Culture no significant growth No debridement was done We offered him HAM 4
5 Case 5 Case 6 Week 40 Week 025 Case 5: 83 year old male Diabetic, CVA, Bed ridden, Hypertensive. Pressure Ulcer at Sacrum stage 3 to 4. X rays no bony involvement. Culture moderate growth of E coli. Initial Debridement was done and standard measures were used to treat ulcer. More than 3 months wound did not heal. We offered him HAM. Dressing change was twice a week with VAC application. Week Week At Week 6 months 80 Case 6: 9 year old male Road side accident. Crush injury to Right foot on medial aspect Skin loss, exposed tendons and fractures of 1 st metatarsal bone Consultation was made and we started HAM. Same week patient was discharged Case 7 HAM-Conclusion Week 40 Week Facilitates epithelialization Maintains a normal epithelial phenotype Infection and rejection of graft are very rare Reduces scarring Reduces tissue adhesion Reduces exudate Case 7: 27 year old male Post RTA. Lacerated Wound Rt. Arm. HAM was applied. Dressing change was 2 times a week 5
6 HAM-Conclusion Inexpensive Widely available Easy to harvest Easy to apply Applied one to 2 times per week References Role of human fetal membrane (Amniotic membrane) in the management of burn wounds annals of burns and fire disasters Vol. XV, 4 th December The Amniotic Membrane: A suitable biological dressing to prevent infection in thermal burns-medical Journal of Islamic Academy of Sciences 13:3, ,2000. Effectiveness of human amnion preserved long term in glycerol as a temporary biological dressing- Burns 25(1999) ) Evaluation of lyophilized, gamma irradiated amnion as a biological dressing- Cells and Tissue Banking 5:73-80,2004 بسم هللا الرحمن الرحيم Thank you 6
Human Tissues Department, Biotechnology Research Center, Tripoli- Libya P.O.Box: 30313,
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