SUPPLEMENTARY INFORMATION In format provided by Isberg et al. (JANUARY 2008)

Transcription

1 Supplementary Table S1: Dot/Icm translocated substrates A: Substrates based on similarity to eukaryotic proteins LepA 1 lpg2793 coiled-coil domain/ bacterial egress CA, BLA LepB 1,2 lpg2490 coiled-coil domain, Rab1 GAP CA bacterial egress LegA2 3 lpg2215 ankyrin repeat BLA LegA3/AnkH lpg2300 ankyrin repeat BLA /AnkW 3-5 LegAS4/AnkI 3,4 lpg1718 ankyrin repeat BLA LegA5/AnkK 3,4 lpg2322 ankyrin repeat BLA LegA7/AnkG/ AnkZ 3-5 lpg0401 ankyrin repeat CA LegA8/AnkN lpg0695 ankyrin repeat BLA /AnkX 3-5 LegA9/AnkY 3,5 lpg0402 ankyrin repeat CA LegA10/ lpg0038 ankyrin repeat CA, BLA AnkQ 3,4,6 LegA11/ lpg0436 ankyrin repeat CA, BLA AnkJ 3,4,6 LegA12/ lpg0483 ankyrin repeat BLA

2 AnkC 3,4 LegA14/Ceg31 lpg2452 ankyrin repeat BLA /AnkF 3,4,7 LegA15/ lpg2456 ankyrin repeat BLA AnkD 3,4 LegAU13/ lpg2144 F-box, ankyrin repeat BLA Ceg27/AnkB 3,4,8 LegC3 3 lpg1701 coiled-coil domain BLA LegC4 3 lpg1953 coiled-coil domain BLA LegC5/Lgt3 3,9 lpg1488 coiled-coil domain; glucosyltransferase of EF1A CA, BLA LegC6 3,10 lpg1588 coiled-coil domain CA, BLA LegC8/Lgt2 3,9 lpg2862 coiled-coil domain; glucosyltransferase of EF1A BLA LegC7/YlfA 3,7 lpg2298 coiled-coil domain CA, BLA LegC2/YlfB 3,7 lpg1884 coiled-coil domain CA, BLA LegG1 3 lpg1976 UVB-resistance protein UVR8 CA, BLA LegG2 3 lpg0276 Ras GEF CA, BLA LegK2 3 lpg2137 serine/threonine protein kinase BLA LegK3 3 lpg2556 serine/threonine protein kinase BLA LegL1 3 lpg0945 leucine-rich repeat BLA

3 LegL2 3 lpg1602 leucine-rich repeat BLA LegL3 3 lpg1660 leucine-rich repeat CA, BLA LegLC4 3 lpg1948 leucine-rich repeat, coiled-coil domain CA, BLA LegL5 3,10 lpg1958 leucine-rich repeat CA, BLA LegL7 3 lpg2400 leucine-rich repeat CA, BLA LegLC8 3,10 lpg1890 leucine-rich repeat, coiled-coil domain CA, BLA LegP 3,11 lpg2999 astacin protease BLA LegS2 3 lpg2176 sphingosine 1-phosphate lyase BLA LegU1 3 lpg0171 F-box BLA LegU2/ lpg2830 U-box; Ubiquitination of Clk CYA, CA, LubX 3,10 BLA

4 B: Substrates identified by directly assaying for Dot/Icm-dependent translocation SidA 12,13 lpg0621 hypothetical protein IT, CA SidB 12,13 lpg1642 Rtx toxin/lipase IT, CA SidC 12,13 lpg2511 PI(4)P binding domain/vesicle trafficking IF, PNS, ST, CA SidD 12,13 lpg2465 hypothetical protein IT, CA SidE 12,13 lpg0234 hypothetical protein IT, CA SdeA 13 lpg0257 hypothetical protein IT SdeC 13 lpg2157 hypothetical protein IT SidF lpg2584 Bcl2-rambo and BNIP3 binding/anti-apoptosis IT, IF, CA SidG 12,13,15 lpg1355 coiled-coil domain CA, IT SidH 12,13 lpg2829 hypothetical protein CA, IT SdhA 16 lpg0376 GRIP domain, coiled-coil domain/anti-apoptosis SE C: Substrates identified in yeast ectopic overexpression studies VipA 17 lpg0390 hypothetical protein /vesicle trafficking CA VipD 17,18 lpg2831 patatin-like phospholipase/vesicle trafficking CA, ST, SE

5 VipF 17 lpg0103 acetyltransferase/vesicle trafficking CA YlfA/LegC7 3,7 lpg2298 coiled-coil domain/vesicle trafficking CA YlfB/LegC2 3,7 lpg1884 coiled-coil domain CA

6 D: Substrates identified based on regulatory networks Ceg7 6 lpg0227 hypothetical protein CA Ceg18 6 lpg0898 hypothetical protein CA Ceg33 6 lpg2591 hypothetical protein CA Ceg10 8 lpg0284 hypothetical protein CA Ceg23 8 lpg1621 hypothetical protein CA Ceg29 8 lpg2409 hypothetical protein CA CegC1 6 lpg0012 hypothetical protein CA CegC2 6 lpg0126 ninein, eukaryotic NIN gene CA CegC3 6 lpg1144 hypothetical protein CA CegC4 6 lpg2200 hypothetical protein CA LegA10/AnkQ 3,4,6 lpg0038 ankyrin repeat CA, BLA LegA11/AnkJ 3,4,6 lpg0436 ankyrin repeat CA, BLA E: Substrate identified by a putative Dot/Icm translocation signal Lpg lpg0045 hypothetical protein CA

7 Lpg lpg0081 hypothetical protein CA Lpg lpg0294 hypothetical protein CA Lpg lpg0365 hypothetical protein CA Lpg lpg0518 hypothetical protein CA Lpg lpg0634 hypothetical protein CA Lpg lpg0963 hypothetical protein CA Lpg lpg1148 hypothetical protein CA Lpg lpg1158 hypothetical protein CA Lpg lpg1273 hypothetical protein CA Lpg lpg1689 hypothetical protein CA Lpg lpg1717 hypothetical protein CA Lpg lpg1751 hypothetical protein CA Lpg lpg2327 hypothetical protein CA Lpg lpg2407 hypothetical protein CA Lpg lpg2527 coiled-coil domain CA Lpg lpg2744 hypothetical protein CA F: Substrates identified by alternative mechanisms

8 RalF 19 lpg1950 Arf1 GEF, ARF1 recruitment CA, IF SidM/DrrA 20,21 lpg2464 Rab1 GEF, Rab1 GDI/Rab1 recruitment CA, IF, PNS LidA 20,22 lpg0940 coiled-coil domain/rab1 sequestering IF, PNS SidJ 9,23 lpg2155 hypothetical protein/vesicle recruitment SE, ST, CA SdjA 9,23 lpg2508 hypothetical protein ST, CA VpdA 18 lpg2410 patatin-like phospholipase SE, ST VpdB 18 lpg1227 patatin-like phospholipase SE, ST WipA 15 lpg2718 hypothetical protein CA WipB 15 lpg0642 hypothetical protein CA a CA: cya-fusion assay; IF: immunofluorescence microscopy; IT: inter-bacterial transfer; PNS: protein present on phagosomes isolated from postnuclear supernatants of infected cells; SE: saponin extraction; ST: SidC-based translocation assay; BLA: fusions to β-lactamase 24. SUPPLEMENTARY TABLE REFERENCES 1. Chen, J. et al. Legionella effectors that promote nonlytic release from protozoa. Science 303, (2004).

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