Table S1 Differentially expressed genes showing > 2 fold changes and p <0.01 for 0.01 mm NS-398, 0.1mM ibuprofen and COX-2 RNAi.

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1 Table S1 Differentially expressed genes showing > 2 fold changes and p <0.01 for 0.01 mm NS-398, 0.1mM ibuprofen and COX-2 RNAi. 0.01mM NS 0.1mM Ibu V vs S Genbank Ratio P-value Ratio P-value Ratio P-value Description BG Adipose differentiation-related protein NM_ angiopoietin-like 4 NM_ interleukin 1 receptor-like 1 NM_ adipose differentiation-related protein NM_ S100 calcium binding protein P NM_ prostaglandin-endoperoxide synthase 2 NM_ dimethylglycine dehydrogenase NM_ matrix metallopeptidase 3 NM_ tumor necrosis factor, alpha-induced protein 6 NM_ solute carrier family 6, member 16 NM_ interleukin 13 receptor, alpha 2 AI Eukaryotic translation initiation factor 3 NM_ GLI pathogenesis-related 1 like 1 CF WDFY family member 4 AW MADS box transcription enhancer factor 2 AW Bcl2 modifying factor BX Phosphodiesterase 11A CA Matrix metallopeptidase 12 (macrophage elastase) NM_ dehydrogenase/reductase (SDR family) member 9 NM_ fibronectin type III domain containing 6 BQ Regulator of G-protein signalling like 1 AF MUSP1 (MUSP1) NM_ similar to POSSIBLE GUSTATORY RECEPTOR NM_ Kruppel-like factor 2 (lung) NM_ adhesion molecule, interacts with CXADR antigen 1 NM_ fibroblast growth factor 1 (acidic) NM_ fibroblast growth factor 12 NM_ proteolipid protein 1 NM_ RAR-related orphan receptor A NM_ cadherin 7, type 2 BX Similar to methylenetetrahydrofolate dehydrogenase 1-like BF Butyrophilin-like 3 NM_ family with sequence similarity 71, member A NM_ solute carrier family 15 NM_ FYN binding protein (FYB-120/130) BF Ectodysplasin A

2 BM Solute carrier family 8 NM_ fms-related tyrosine kinase 1 AW Cancer susceptibility candidate 5 NM_ fatty acid binding protein 4, adipocyte NM_ aldo-keto reductase family 1, member B1 BM High-mobility group box 4 BM Rho GTPase activating protein 29 AI V-ets erythroblastosis virus E26 oncogene homolog 1 BC Homo sapiens, clone IMAGE: , mrna AK Ribosomal protein S6 BM Solute carrier family 4 BX Integrin, alpha 2 AB myosin, heavy polypeptide 15 AA Carnitine palmitoyltransferase 1A (liver) AA Ubiquitin specific peptidase 24 H SH3-domain GRB2-like 1 AW EPH receptor B2 NM_ taste receptor, type 2, member 3 NM_ fibroblast growth factor 14 AI Hemochromatosis NM_ zinc finger protein 533 NM_ ADAMTS-like 4 NM_ inversin CB Peroxisome biogenesis factor 26 NM_ involucrin BU RAN binding protein 9 BX Zinc finger protein 491 BC hypothetical protein LOC NM_ interleukin 1 family, member 9 BC Homo sapiens cdna clone IMAGE: NM_ solute carrier family 30 NM_ KIAA1387 protein AV Full length insert cdna YI01B02 BX KIAA1505 protein AK mitochondrial ribosomal protein L46 AK Solute carrier family 7 member 1 NM_ glypican 4 NM_ progestagen-associated endometrial protein BE Chromosome 5 open reading frame 21 AI Ataxin 1 AA Hepatitis B virus x interacting protein

3 BE Kinesin family member 22 BF Holocarboxylase synthetase NM_ anterior gradient 2 homolog (Xenopus laevis) NM_ special AT-rich sequence binding protein 1 BI Thrombospondin 1 NM_ MORN repeat containing 3 NM_ serum/glucocorticoid regulated kinase AL Protein tyrosine phosphatase, receptor type, J BX Polycystic kidney disease 2 (autosomal dominant) NM_ major histocompatibility complex, class II BU Membrane associated guanylate kinase AA Chemokine-like factor NM_ ADAM metallopeptidase with thrombospondin type 1 NM_ membrane protein, palmitoylated 4 NM_ vasohibin 2 NM_ cation channel, sperm associated 2 NM_ gamma-glutamyltransferase-like activity 1 AI Adaptor-related protein complex 2, mu 1 subunit NM_ chitinase 3-like 1 (cartilage glycoprotein-39) NM_ sprouty homolog 1, antagonist of FGF signaling AI Stress-associated endoplasmic reticulum protein 1 CD Leucine rich repeat (in FLII) interacting protein 2 BF Albumin NM_ chromosome 9 open reading frame 26 (NF-HEV) NM_ signal-regulatory protein gamma NM_ SAM pointed domain containing ets transcription factor NM_ serpin peptidase inhibitor, clade A NM_ tumor necrosis factor receptor superfamily NM_ COMM domain containing 4 BF Ubiquitin protein ligase E3A

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Supplementary Table SI: Y strain T. cruzi infection results in the upregulation of 381 genes at the site of infection.

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