CURRICULUM VITAE. January 2014-now: PhD student in Molecular Medicine. Turin Medical School University, Italy.

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1 First name and surname: Antonio D Ambrosio. CURRICULUM VITAE Place and date of birth: Torino, Italy. September 17, Citizenship: Italian. Work Adress: Institute for Cancer Research at Candiolo (IRCC), University of Turin Medical School,Sp.142 km 3, Candiolo (TO), Italy. antonio.dambrosio@ircc.it Education: January 2014-now: PhD student in Molecular Medicine. Turin Medical School University, Italy. February 2013: Licence to practice Medicine and Surgery. Member of the Turin College of Physicians. October 2012: Master s Degree in Medicine and Surgery (M.D.) Score: 110/110. Turin Medical School University, Italy; Title of the thesis: Characterization of glioblastoma stem cell for diagnostic and therapeutical purpouses. July 2000: High School Certificate in Chemistry (I.T.I.S.). Score: 100/100. I.T.I.S. Luigi Casale. Torino, Italy. Research experience: January 2014-now: PhD student at Cancer stem cell research laboratory Deptl. Experimental Clinical Molecular Oncology (PI: Prof. Carla Boccaccio). IRCC - Candiolo (TO), Italy; March 2013-Dicember 2013: post-degree fellow at Cancer stem cell research laboratory Deptl. Experimental Clinical Molecular Oncology (PI: Prof. Carla Boccaccio). IRCC - Candiolo (TO), Italy; Program of study: Targeting the MET oncogene to overcome glioblastoma stem cell radioresistance. March 2010-January 2013: Internal student: in vivo and in vitro laboratory activities in cancer stem cells biology at Cancer stem cell research laboratory Deptl. Experimental Clinical Molecular Oncology (PI: Prof. Carla Boccaccio). IRCC - Candiolo (TO), Italy. Technical skills and Competences: Cell Biology: cell culture for immortalized cell lines and tumor derived cancer stem cells; biological assays for evaluation of cellular motility and invasion, clonogenic ability, proliferation, viability and apoptosis. Isolation of cancer stem-like cells from tumor human specimens and xenografts. In vitro sphere-forming and limiting dilution analysis (LDA) for the evaluation of cancer stem cell frequency. Molecular Biology: Genomic DNA extraction, total RNA extraction, RT-PCR, PCR, copy number evaluation and techniques for gene cloning and expression; transfection of lentiviral vectors in

2 immortalized cell lines and tumor derived cancer stem cells. Protein extraction from cells and tumor tissues; western blot analysis. In vivo competences: mice manipulation and basic surgical techniques; subcutaneous and intracranial stereotactic transplantations of immortalized cell lines and tumor derived cancer stem cells; tail vein injections of immortalized cell lines and tumor derived cancer stem cells. In vivo limiting dilution analysis (LDA). Generation of patient derived xenografts (PDX): glioblastoma, CUP and breast cancer models. Small animal molecular imaging: bioluminescence, fluorescence and microct scanning. Irradiation of preclinical mouse models with clinical devices (linear irradiator; tomotherapy Hi-Art). Histopathology: histological and immunoistochemical (IHC)/immunofluorescence (IF) analysis of the tissue samples derived from human specimens and xenografts. Bio-informatic: good use of Microsoft Word, Excel, PowerPoint, Adobe Photoshop, Adobe Illustrator, ImageJ, Prism software and statistical analysis. Languages: mother tongue: Italian; other language(s): English. Awards: January 2014-now: Scholarship provided by Compagnia di San Paolo University of Torino. March 2013-Dicember 2013: fellowship provided by F.P.O. (Fondazione Piemontese per l Oncologia); IRCC Candiolo (TO), Italy. Publications: Miranti, A. D Ambrosio, G. Cattari, E. Garibaldi, S. Bresciani, P. Gabriele, M. Stasi. NOD-SCID mice irradiation with Tomotherapy and TrueBeam STx: dosimetric and radiobiological results Physica Medica: European Journal of Medical Physics (2016) under revision; De Bacco F, D Ambrosio A, Casanova E, Orzan F, Neggia R, Albano R, Verginelli F, Cominelli M, Poliani PL, Luraghi P, Reato G, Pellegatta S, Finocchiaro G, Perera T, Garibaldi E, Gabriele P, Comoglio PM, Boccaccio C. MET inhibition overcomes radiation resistance of glioblastoma stem-like cells EMBO Molecular Medicine (2016) 2;8 (5): ; De Bacco F, Casanova E, Medico E, Pellegatta S, Orzan F, Albano R, Luraghi P, Reato G, D'Ambrosio A, Porrati P, Patane M, Maderna E, Pollo B, Comoglio PM, Finocchiaro G, Boccaccio C. The MET oncogene is a functional marker of a glioblastoma stem cell subtype Cancer Research (2012) 72(17): Abstracts and participation to national and international congresses: 2016: Cancer Stem Cells (CSC): Impact on Treatment 7-11 December 2016, Obergulg (Austria)- Abstract and poster:

3 MET inhibition overcomes radiation resistance of glioblastoma stem-like cells De Bacco Francesca, D'Ambrosio Antonio, Casanova Elena, Orzan Francesca, Neggia Roberta, Albano Raffaella, Verginelli Federica, Cominelli Manuela, Poliani Pietro L., Luraghi Paolo, Reato Gigliola, Pellegatta Serena, Finocchiaro Gaetano, Perera Timothy, Garibaldi Elisabetta, Gabriele Pietro, Comoglio Paolo M., Boccaccio Carla. ESMO October 2016, Copenhagen (Denmark) -Abstract and poster: AGNOSTOS precision medicine project in patients with Cancer of Unknown Primary (CUP) E. Geuna, S. Benvenuti, F. Verginelli, D. Galizia, S. Siena, G. Stella, A. Gentile, M. Milan, A. Virzì, A. D Ambrosio, P. Cassoni, R. Senetta, A. Balsamo, M. Spione, A. Nuzzo, A. Sapino, S. Marsoni, C. Boccaccio, P.M. Comoglio, F. Montemurro. 58th Annual Meeting of the Italian Cancer Society 5-8 September 2016, Verona (Italy) Abstract and poster: MET inhibition overcomes radiation resistance of glioblastoma stem-like cells Francesca De Bacco, Antonio D Ambrosio, Elena Casanova, Francesca Orzan, Roberta Neggia, Raffaella Albano, Federica Verginelli, Manuela Cominelli, Pietro L. Poliani, Paolo Luraghi, Gigliola Reato, Serena Pellegatta, Gaetano Finocchiaro, Timothy Perera, Elisabetta Garibaldi, Pietro Gabriele, Paolo M. Comoglio and Carla Boccaccio. ESTRO 35 CONGRESS 29 april-3 may 2016, Torino (Italy) Abstract and poster: Small animal irradiation by using tomotherapy: dosimetric and preclinical results Miranti A, D'Ambrosio A, Cattari G, Garibaldi E, Bresciani S, Gabriele P, Stasi M. AIFM february 2016, Perugia (Italy) Abstract: Dosimetric and preclinical results of small animal irradiation by using tomotherapy A. Miranti, A. D'Ambrosio, G. Cattari, S. Bresciani, M. Poli, C. Cutaia, P. Gabriele, M. Stasi. 2015: - GINP -IL GLIOBLASTOMA. RIUNIONE DEL GRUPPO ITALIANO DI NEUROPATOLOGIA(GINP) - November A.O. Spedali Civili di Brescia, Brescia (Italy); - ESMO MAP 2015 Molecular Analysis for personalized therapy October 2015, Paris (France); - GBM II International Symposium on Clinical and Basic Investigation in Glioblastoma september 2015, Toledo (Spain) Abstract and poster: "MET inhibition overcomes radiation resistance of glioblastoma stem-like cells" Francesca De Bacco, Antonio D Ambrosio, Elena Casanova, Roberta Neggia, Raffaella Albano, Francesca Orzan, Federica Verginelli, Gigliola Reato, Paolo Luraghi, Serena Pellegatta, Gaetano Finocchiaro, Elisabetta Garibaldi, Pietro Gabriele, Paolo M. Comoglio and Carla Boccaccio. - CYTO 2015-XXX Congress of the International Society for Advancement of Cytometry june 2015, Glasgow (UK) Abstract and poster: The low-affinity neurotrophin receptor p75 identifies cell line subpopulations displaying stem-like properties in vitro and increased tumorigenicity in vivo Casanova E., De Bacco F., Angelini P., Geuna M., Neggia R., D Ambrosio A., Reato G., Luraghi P., Pisacane A., Comoglio P.M., Boccaccio C. - ISSCR 2015, june 2015, Stockholm (Sweden) Abstract and poster: Targeting the MET oncogene overcomes Glioblastoma Stem-like Cell radioresistance Francesca De Bacco,

4 Antonio D Ambrosio, Elena Casanova, Roberta Neggia, Raffaella Albano, Francesca Orzan, Federica Verginelli, Gigliola Reato, Paolo Luraghi, Serena Pellegatta, Gaetano Finocchiaro, Elisabetta Garibaldi, Pietro Gabriele, Paolo M. Comoglio and Carla Boccaccio. - EACR-AACR-SIC Special Conference on Anticancer Drug Action and Drug Resistance:from Cancer Biology to the Clinic june 2015, Firenze (Italy) Abstract and poster: Targeting the MET oncogene overcomes Glioblastoma Stem-like Cell radioresistance Francesca De Bacco, Antonio D Ambrosio, Elena Casanova, Roberta Neggia, Raffaella Albano, Francesca Orzan, Federica Verginelli, Gigliola Reato, Paolo Luraghi, Serena Pellegatta, Gaetano Finocchiaro, Elisabetta Garibaldi, Pietro Gabriele, Paolo M. Comoglio and Carla Boccaccio. - IRCCS International Conference - Molecular Oncology From Signal Transduction to Cancer Precision Medicine' 6-7 june 2015, Candiolo (Italy) Abstract and poster: MET inhibitions impairs radiation resistance of glioblastoma stem-like cells Francesca De Bacco, Antonio D Ambrosio, Elena Casanova, Roberta Neggia, Raffaella Albano, Francesca Orzan, Federica Verginelli, Gigliola Reato, Paolo Luraghi, Serena Pellegatta, Gaetano Finocchiaro, Elisabetta Garibaldi, Pietro Gabriele, Paolo M. Comoglio and Carla Boccaccio. 2014: - IRCCS Candiolo Cancer Institute MCO October , Torino (Italy) Abstract and poster: Irradiation of preclinical models: proposal of a new technique Elisabetta Garibaldi, Gabriella Cattari, Anna Miranti, Claudia Cutaia, Antonio D Ambrosio, Francesca De Bacco, Carla Boccaccio and Pietro Gabriele. - IRCCS Candiolo Cancer Institute MCO October , Torino (Italy) Abstract and poster: MET signaling sustains Glioblastoma Stem-like Cells radioresistance Francesca De Bacco, Antonio D Ambrosio, Elena Casanova, Francesca Orzan, Raffaella Albano, Serena Pellegatta, Roberta Neggia, Gigliola Reato, Gaetano Finocchiaro, Paolo M. Comoglio and Carla Boccaccio. - EMBO Cancer stem cells 20 years later: Achievements, controversies, emerging concepts and technologies - October , Catanzaro (Italy). - EACR23, July Munich (Germany) - Abstract and poster: MET signaling sustains Glioblastoma Stem-like Cells radioresistance Francesca De Bacco, Antonio D Ambrosio, Elena Casanova, Francesca Orzan, Raffaella Albano, Serena Pellegatta, Roberta Neggia, Gigliola Reato, Gaetano Finocchiaro, Paolo M. Comoglio and Carla Boccaccio. 2013: GIC XXXI Cytometry National Conference La Citometria dalla ricerca alla clinica October , Lucca (Italy) -Abstract and poster: "The MET Oncogene Is a Functional Marker of a Glioblastoma Stem Cell Subtype" De Bacco F., Casanova E., Medico E., Pellegatta S.,Orzan F., Albano R., D Ambrosio A., Pollo B., Comoglio P.M., Finocchiaro G., Boccaccio C. ISCRR 11th annual meeting - June Boston, MA (USA) - Abstract and poster: The MET oncogene is a functional marker of a glioblastoma stem cell subtype and a therapeutical

5 target for radiosensitization Boccaccio Carla, De Bacco Francesca, Casanova Elena, Medico Enzo, Pellegatta Serena,Orzan Francesca, Albano Raffaella, Luraghi Paolo, D'Ambrosio Antonio, Pollo Bianca, Comoglio Paolo M.,Finocchiaro Gaetano. ESTRO (European Society for Radiotherapy and Oncology) Forum - April , Geneva (Switzerland) - Abstract and poster: "Targeting the MET oncogene to radiosensitize cancer stem cells" Boccaccio C., De Bacco F., Casanova E., Orzan F., D Ambrosio A., Gabriele P., Pellegatta S., Finocchiaro G., Comoglio P.M. 2012: EACR 22nd Congress - July , Barcelona (Spain) - Abstract and poster: "Tyrosine Kinase Receptors as Functional Markers and Therapeutical Targets of Glioblastoma Stem Cells" De Bacco F., Casanova E., Medico E., Pellegatta S., Orzan F., Albano R.,D Ambrosio A., Pollo B., Finocchiaro G., Boccaccio C. 2011: IRCC International Conference Molecular Clinical Oncology - May , Torino (Italy).