Tushar Tomar. Current Position Postdoctoral researcher, Department of Medical Oncology, University Medical Center Groningen, The Netherlands
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1 Tushar Tomar Department of Medical Oncology, Cancer Research Center Groningen (CRCG) University Medical Center Groningen (UMCG) University of Groningen (RuG) 9713 GZ Groningen, The Netherlands Present Current Position Postdoctoral researcher, Department of Medical Oncology, University Medical Center Groningen, The Netherlands Academic Credential PhD in Medical Sciences, Department of Medical Oncology, University Medical Center Groningen, The Netherlands Masters in Medical and Pharmaceutical Sciences, University of Groningen, The Netherlands Bachelor in Pharmacy, Delhi Institute of Pharmaceutical Sciences and Research, University of Delhi, New Delhi, INDIA Academic Accomplishments/Awards Jan Kornelis de Cock Stichting Grant with a financial support of 4,500 euros Jan Kornelis de Cock Stichting Grant with a financial support of 4,000 euros EACR Proffered Paper Award 2014 for elected as best abstract in Imaging session at European Association of Cancer Research Biennial Meeting 2014, Munich Germany Obstetrics and Gynecology, UMCG Grant 2013 for pilot study with a financial support of 5,000 euros for research project Jan Kornelis de Cock Stichting Grant with a financial support of 3,000 euros Graduate School for Drug Exploration (GUIDE) Master's Scholarship, University of Groningen, The Netherlands which included tuition fees and living cost National Institute of Pharmaceutical sciences and research (NIPER) Entrance Exam 2009, India (ranked in Top 2% qualified students) Topper for attaining highest marks in Biology (98%) in Pre-university Exam, 2005 at all India level 1 P a g e
2 2016-Present Project: Project 1: Project 2: Project 1: Project 2: 1) 2) 3) 4) 5) 6) Research Experience Postdoctoral researcher, Department of Medical Oncology, University Medical Center Groningen, The Netherlands Prof. F.A.E. Kruyt and Prof. S. de Jong Targeting cancer stem cells in breast cancer model using novel antibody-drug conjugates PhD Scholar, Department of Gynaecological Oncology, University Medical Center Groningen, The Netherlands Prof. S. de Jong, Prof. A.G.J. van der Zee and Dr. G.B.A. Wisman Identification and functional validation of novel DNA methylation markers in relation to platinum-based chemotherapy in ovarian cancer Establishment of Patient-derived xenograft (PDX) mouse model for ovarian cancer and characterize them for their utility for novel target validation and treatment options Master s research Projects, University of Groningen, The Netherlands Targeting of anti-tumor Interferon-gamma to Tumor Stromal Fibroblasts through PDGF receptor (9 months) Prof. K.Poelstra and Dr. J. Prakash Effects of high fat-diet on renal endothelial dysfunction in Human CRP tansgenic mice (6 months) Prof. P. Heeringa and G.K. Yakala (PhD Student) Publications PhD doctoral thesis with title (Epi)genomics characterization of chemoresponse in ovarian cancer: finding better markers, model and targets for therapy, 2016 Tomar T, de Jong S, Alkema NG, Meersma GJ, Klip HG, van der Zee AGJ, Wisman GBA; Genome-wide methylation profiling of ovarian cancer patient-derived xenograft (PDXs) treated with the demethylating agent decitabine identifies novel epigenetically regulated genes and pathways (accepted in Genome Medicine) Tomar T*, Alkema NG*, Duiker EW, Meersma GJ, Klip HG, van der Zee AGJ, Wisman GBA, de Jong S; Biobanking of patient and patient-derived xenograft (PDX) ovarian tumour tissue: efficient preservation with low and high FCS based methods, Scientific Reports Oct Joseph JV, Tomar T*, Conroy S*, Eggens-Meijer E, Bhat K, Copray S, Walenkamp AM, Boddeke E, Balasubramanyian V, Wagemakers M, den Dunnen WF, Kruyt FA; TGF-β is an inducer of ZEB1-dependent mesenchymal transdifferentiation in glioblastoma that is associated with tumor invasion, Cell Death Dis Oct 2;5:e1443 Tomar T*, Alkema NG*, van der Zee AGJ, Everts M, Meersma GJ, Hollema H, de Jong S, van Vugt MA*, Wisman GBA*; Checkpoint kinase 2 (Chk2) supports sensitivity to platinum-based treatment in high grade serous ovarian cancer; Gynecol Oncol Jun;133(3):591-8 Bansal R, Tomar T, Ostman A, Poelstra K, Prakash J; Selective targeting of interferon gamma to stromal fibroblasts and pericytes as a novel therapeutic approach to inhibit angiogenesis and tumor growth, Mol Cancer Ther Nov;11(11): P a g e
3 Laboratory Experience Epigenetic methylation assays: Infinium methylation array (450K and 27K), Bisulphite pyrosequencing, Methylation specific PCR (MSP), Quantitative MSP, Genomic data analyses: SNP Arrays (Infinium), Microarrays (Operon V3 & Affymetrix HU133A), Quantitative rt-pcr Genome Editing: Molecular cloning, Transient knock-downs via sirnas, stable knockdowns by ShRNA and overexpression by pcdna transfection; CRISPRi and CRISPR-Cas9 genome editing Biochemical assays: Drug cytotoxicity assays (MTT, MTS, Alamar blue), ELISA, and Western Blotting, Cytokine bead array assay (FACS), multi-color cell surface FACS Cell culturing (primary and immortalized tumor cell lines [2D monolayer and 3D spheroids], endothelial cells, fibroblasts cell lines) and various cellular assay (proliferation, apoptosis, wound healing assay, tube formation assay, migration and invasion assay, cell cycle and DNA damage analysis (FACS), fluorescence and cytochemical staining, tissue stainings (including fluorescence staining) In-vivo experiments: Establishing Patient-derived xenograft (PDX) mouse model for ovarian cancer, various cell lines-based xenograft models, in-vivo molecular optical imaging, in-vivo drug testing Sept June present Teaching and Supervisory Experience Course instructor: Involved in University Course Research Proposal Cancer Research 2016/2017 (WMBM15001), at department of Medical Oncology, University Medical Center Groningen Course instructor: Organized lab research module for International Oncology Summer School 2016, at department of Medical Oncology, University Medical Center Groningen Laboratory supervisor: Supervision of three master degree students project for 6 months ( ) and 9 months ( ) and 9 months ( ) Daily supervisor: Trained four undergraduate students for 2 months short research project at medical oncology lab Courses Cancer Biology, Introduction into clinical and fundamental Oncology (organized by Dutch Society of Oncology, NVvO), In-vivo molecular optical imaging, Cellular and molecular biology, Pharmacology, Pathophysiology & toxicology and Biotechnology Additional Courses: Laboratory animal course (Article 9), Microbiology safety course (Dutch legislation complied), Data analysis using R language, Basic Medical Statistics, Project management, Publishing in English writing course, Grant proposal writing, Presentation skills 3 P a g e
4 2014-present Participation in Peer Review I reviewed articles for these scientific journals: Cancer letters; Cell death and diseases 2012-present 2013-present 2014-present present Memberships American Association for Cancer Research (AACR) Associate membership European Association for Cancer Research (EACR) PhD student ambassador Cancer Research Center Groningen (CRCG) Student membership Graduate School of Medical Sciences (GSMS) PhD Student membership Graduate School of Medical Sciences (GSMS) PhD Development Conference Finance manager in organization committee Glasgow- Groningen Joint Post-doc meeting 2015 Organization committee member Groningen Indian Students Association (GISA) President ( ) and Advisory board member ( ) University of Groningen (RuG) Alumni board University student ambassador for India 10/ / / / /2013 Presentations at Conferences/Seminars Oral presentation, Evaluation of the ovarian cancer patient-derived xenograft (PDXs) methylome for cancer epigenetics studies, 3 rd Annual PhD student meeting, Cancer Research Center Groningen (CRCG), Groningen, The Netherlands Invited short talk, Dual wavelength near-infrared fluorescence imaging of VEGF and IGF-1R in ovarian cancer patient-derived xenografts (PDX) model, Glasgow-Groningen (G2) joint post-doc meeting 2015, at Glasgow, UK Oral presentation, Genome-wide integrated epigenomics identifies FZD10 as novel modulator for platinum sensitivity in high grade serous ovarian cancer, Tumor cell biology annual meeting, Dutch Cancer Society, Lunteren, The Netherlands Invited short talk, Dual wavelength near-infrared fluorescence imaging of VEGF and IGF-1R in ovarian cancer patient-derived xenografts (PDX) model, European Association of Cancer Research (EACR) biennial Meeting 2014, at Munich, Germany Oral presentation, Identification of FAM as novel epigenetic chemoresponse marker for platinum-based chemotherapy resistance in high grade serous ovarian cancer, 1 st Annual PhD student meeting, Cancer Research Center Groningen (CRCG), Groningen, The Netherlands 4 P a g e
5 10/ /2013 Poster presentation, Identification of novel epigenetic biomarkers for platinum-based chemotherapy resistance in high grade serous ovarian cancer, Cell Symposium on Cancer Epigenomics at Sitges, Spain Poster presentation, Identification of novel epigenetic biomarkers for platinum-based chemotherapy resistance in high grade serous ovarian cancer, American Association for Cancer Research (AACR), Washington DC, USA References Available upon request TUSHAR TOMAR Place: Groningen, The Netherlands 5 P a g e
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