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![( 473000). [J]. 2016 20(14)](/docs-images/90/102843958/images/1-1.jpg ":2033-2039. DOI: 10.3969/j.issn.2095-4344.2016.14.")
1 Chinese Journal of Tissue Engineering Research April 1, 2016 Vol.20, No.14 ( ). [J] (14): DOI: /j.issn ORCID: () Transwell RT-PCR caspase-3 mrna Western blot caspase-3 IgG Fc IgG IgG Fc :R394.2 :A : (2016) CD h RT-PCR Western blot caspase-3 mrna (P < 0.05) caspase-3 mrna (P < 0.05) caspase-3 mrna caspase-3 ISSN CN /R CODEN: ZLKHAH 2033
2 . Shan Hai-xia, Master, Attending physician, Department of Infectious Diseases, Nanyang Central Hospital, Nanyang , Henan Province, China Corresponding author: Zhai Yu-feng, Chief physician, Department of Infectious Diseases, Nanyang Central Hospital, Nanyang , Henan Province, China Cytobine-induced killer cells promote apoptosis of human liver cancer stem cells Shan Hai-xia, Fan Chong-gui, Huo Li-ya, Zhang Huai-hong, Zhai Yu-feng (Department of Infectious Diseases, Nanyang Central Hospital, Nanyang , Henan Province, China) Abstract BACKGROUND: Immunotherapy with autologous immune cells has been developed as a major adjuvant therapy for malignant tumors, but its mechanism of action has not been elucidated. OBJECTIVE: To investigate the relationship between cytokine-induced killer cell secretion and apoptosis in human liver cancer stem cells. METHODS: Human liver cancer stem cells, HepG2 cells, were isolated and enriched using serum-free suspension method. The peripheral blood mononuclear cells from patients with liver cancer were induced by γ-interferon, CD3 monoclonal antibody and recombinant human interleukin-2 to form killer cells. Passage 1 liver cancer stem cells were divided into control group (culture alone) and experimental group (co-culture of cytokines-induced killer cells and human liver cancer stem cells). At 48 hours after culture, apoptosis in human liver cancer stem cells was detected using flow cytometry, and expression of caspase-3 mrna and protein was detected using RT-PCR and western blot, respectively. RESULTS AND CONCLUSION: The apoptotic rate in the control group was significantly lower than that in the experimental group (P < 0.05). The expressions of caspase-3 at mrna and protein levels were both higher in the experimental group than the control group (P < 0.05). Experimental findings show that cytokines-induced killer cells can significantly promote apoptosis in human liver cancer stem cells, and up-regulate the caspase-3 mrna and protein expressions dramatically. Subject headings: Liver Neoplasms; Neoplastic Stem Cells; Cytokine-Induced Killer Cells; Apoptosis; Tissue Engineering Cite this article: Shan HX, Fan CG, Huo LY, Zhang HH, Zhai YF. Cytobine-induced killer cells promote apoptosis of human liver cancer stem cells. Zhongguo Zuzhi Gongcheng Yanjiu. 2016;20(14): Introduction % [1] [2] T [3-4] [5] [6] [7] 1Materials and methods HepG2 DMEM ( ) ( )( ( )) GAPDH ( ) B27( 2034 P.O. Box 10002, Shenyang
3 . ) () () ( ) caspase-3 ( ) ( )2( ) () PBS () ( ) RPMI 1640( ) IgG( ) () (Bio-Rad ) (Promega ) CO 2 ( ) Transwell ( ) ( ( ) ) () HepG2 DMEM 37 5%CO 2 ( 20 µg/l 20 µg/l 2% B27 20 µg/l 100 U/mL 100 U/mL )37 5%CO 2 2 d 1 14 d 7 d 1 3 CD133 CD90 1 h PBS 3 PBS CD90 CD RPMI L 1 1 dcd3 2 3 d 1 RPMI d L ( L 1 ) Transwell d 450 nm h PBS L µl Annexin -FITC 10 min RT-PCR caspase-3 mrna 48 h RNAcDNARNA cdna caspase-3 mrna GAPDH GAPDH mrna 1 1 Table 1 Primer sequences of different genes GAPDH ( ) caspase-3 5 -AAC GGA TTT GGT CGT ATT G-3 5 -TGG CAT TGA GAC AGA CAG-3 5 -CTT CTG GGT GGC AGT GAT-3 5 -AAA GTA GCG TCA AAG GAA Western blot caspase-3 48 h SDS 5% 45 mincaspase-3 4 IgG 2 hgapdh 1.5 caspase-3 mrna ISSN CN /R CODEN: ZLKHAH 2035
4 . A B C 1 Figure 1 Cultivation and identification of human liver cancer stem cells A 14 d( 200) B C 3 CD90 CD133 95% A B C 2 Figure 2 Cytokine-induced killer cell culture and morphology A 1 d( 100) B 3 d( 100) C 7 d ( 400) 1.6 SPSS 19.0 x _ ±st P < Results d ( 1) 3CD90 CD133 95% d (P < 0.05) 2 (x _ ±s) Table 2 Absorbance values of human liver cancer stem cells in the two groups at different time of culture 1 d 3 d 5 d 7 d 0.64± ± ± ± ± ± ± ±0.04 P < 0.05 < 0.05 < 0.05 < 0.05 ( 2A)3 d ( 2B)7 d ( 2C) d (P < 0.05) (0.065± 0.007)% (12.145±1.025)% 0 1 d 3 d 5 d 7 d 3 Figure 3 Absorbance values of human liver cancer stem cells in the two groups at different time of culture 2036 P.O. Box 10002, Shenyang
5 . caspase bp GAPDH M r GAPDH 534 bp caspase-3 M r RT-PCR caspase-3 mrna Figure 4 Expression of caspase-3 mrna in human liver cancer stem cells detected by RT-PCR 2.5 caspase-3 mrna RT-PCR caspase-3 mrna (P < 0.05)4 2.6 caspase-3 caspase-3 (P < 0.05)5 3Discussion [8] [9-11] [12] [13-15] [16-18] [19-23] Rosenberg [24] 5 caspase-3 Figure 5 Expression of caspase-3 protein in human liver cancer stem cells detected by western blot T 2 Grimm [25] Gutgemann [26] [27] [28] [29] [30] [31] caspase [32] caspase-3 [33-35] caspase-3 caspase-3 [36-38] 2 1 [39] caspase-3 [40] ISSN CN /R CODEN: ZLKHAH 2037
6 . caspase-3 mrna (P < 0.05) caspase-3 mrna 2009 Ethical issues in animal experimentation CNKI 3 ( ) 4 References [1],,,. [C] ,2014: [2],,,.DC+CIK [C].2009,2009: [3],,,. (DC) CIK [J].:,2012,9: [4],,,. CIK [J].,2011,20(2): [5] Zhu R, Mancini-Bourgine M, Zhang XM, et al. Plasmid vector-linked maturation of natural killer (NK) cells is coupled to antigen-dependent NK cell activation during DNA-based immunization in mice. J Virol. 2011;85(19): [6] Smyth MJ, Cretney E, Takeda K, et al. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) contributes to interferon gamma-dependent natural killer cell protection from tumor metastasis. J Exp Med. 2001;193(6): [7],,,. CIK [J].,2009,11(10): [8],,,.DC CIK HuH-7[J].,2014,21(5): [9],,,. [J].,2014,13(5): [10],,,.IL-21 [J]., 2012,33(10): [11] Muench MO, Humeau L, Paek B, et al. Differential effects of interleukin-3, interleukin-7, interleukin 15, and granulocyte-macrophage colony-stimulating factor in the generation of natural killer and B cells from primitive human fetal liver progenitors. Exp Hematol. 2000;28(8): [12],,,. [J].,2004,27(5): [13] Huang J, Li C, Wang Y, et al. Cytokine-induced killer (CIK) cells bound with anti-cd3/anti-cd133 bispecific antibodies target CD133(high) cancer stem cells in vitro and in vivo. Clin Immunol. 2013;149(1): [14],,,. [J]., 2007, 28(3): [15] Yuanying Y, Lizhi N, Feng M, et al. Therapeutic outcomes of combining cryotherapy, chemotherapy and DC-CIK immunotherapy in the treatment of metastatic non-small cell lung cancer. Cryobiology. 2013;67(2): [16],,,.CIK [J].,2009,19(21): ,3226. [17],.DC CIK [J].,2015,12(2): [18],.DC-CIK [J].,2011,27(6): [19],,,. [J]., 2009,19(19): [20],,,. [J].,2008,24(2): , P.O. Box 10002, Shenyang
7 . [21],,,. [J].,2014,22(2): [22],. [J].,2014,11(2): [23],. [J].,2011, 21(1): [24] Rosenberg SA, Lotze MT, Muul LM, et al. Observations on the systemic administration of autologous lymphokine-activated killer cells and recombinant interleukin-2 to patients with metastatic cancer. N Engl J Med. 1985;313(23): [25] Grimm EA, Mazumder A, Zhang HZ, et al. Lymphokine-activated killer cell phenomenon. Lysis of natural killer-resistant fresh solid tumor cells by interleukin 2-activated autologous human peripheral blood lymphocytes. J Exp Med. 1982;155(6): [26] Gütgemann S, Frank S, Strehl J, et al. Cytokine-induced killer cells are type II natural killer T cells. Ger Med Sci. 2007;5:Doc07. [27],,,. [J].,2006,86(26): [28],,. DC-CIK 531 [J].,2011,27(4): [29],,,. DC-CIK 110 [J].,2010,30(8): [30],,,. [J].,2003,83(23): [31] Marin V, Pizzitola I, Agostoni V, et al. Cytokine-induced killer cells for cell therapy of acute myeloid leukemia: improvement of their immune activity by expression of CD33-specific chimeric receptors. Haematologica. 2010;95(12): [32] Motamedi M, Arab S, Moazzeni SM, et al. Improvement of a dendritic cell-based therapeutic cancer vaccine with components of Toxoplasma gondii. Clin Vaccine Immunol. 2009;16(10): [33] Lin CC, Yu YL, Shih CC, et al. A novel adjuvant Ling Zhi-8 enhances the efficacy of DNA cancer vaccine by activating dendritic cells. Cancer Immunol Immunother. 2011;60(7): [34],,,. [J]., 2006,25(11): [35],,,.DC-CIK [J].,2010,17(1): [36] Xie L, Pang R, Jin Y, et al. Effects of hepatic artery chemotherapeutic embolization combined with perfusing LAK cells into hepatic artery after radical operation of liver cancer. Zhonghua Gan Zang Bing Za Zhi. 2000;8(3): [37] Li H, Yu JP, Cao S, et al. CD4 +CD25 + regulatory T cells decreased the antitumor activity of cytokine-induced killer (CIK) cells of lung cancer patients. J Clin Immunol. 2007;27(3): [38] Ren J, Di L, Song G, et al. Selections of appropriate regimen of high-dose chemotherapy combined with adoptive cellular therapy with dendritic and cytokine-induced killer cells improved progression-free and overall survival in patients with metastatic breast cancer: reargument of such contentious therapeutic preferences.clin Transl Oncol. 2013;15(10): [39],,,.DC CIK [J].,2014, 34(1): [40],,,. [J]., 2004,26(8): ISSN CN /R CODEN: ZLKHAH 2039
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