Gary Kupfer, M.D. CURRICULUM VITAE

Transcription

1 CURRICULUM VITAE Name: Education: BS, microbiology, University of Florida, 1983 MD, Johns Hopkins University, 1989 Fellow, Pediatric Hematology/Oncology, Boston Children s Hospital, Harvard Medical School, 1995 Career/Academic Appointments: Research Associate, Laboratory of Dr. Warren Ross, University of Florida, Gainesville, FL. Project: Molecular Mechanisms of Chemotherapy Drugs Research Associate. Laboratory of Dr. Leroy Liu, John Hopkins School of Medicine, Baltimore, MD. Project: Molecular Mechanisms of Topoisomerases Research Associate, Laboratory of Dr. Chaim Kahane, Weizmann Institute, Rehovot, Israel. Project: Cloning and Sequencing Human Ornithine Decarboxylase Gene Research Associate, Laboratory of Dr. Robert Casero, John Hopkins School of Medicine Baltimore, MD. Project: Polyamine Metabolism in Carcinogenesis Resident in Pediatrics, Children s Hospital of Philadelphia Coordinator, Homeless Health Project, Children s Hospital at Philadelphia Fellow in Medicine (Hematology/Oncology), Children s Hospital, Boston Post-doctoral Fellow, Laboratory of Dr. Alan D. D Andrea, Dana-Farber Cancer Institute, Boston, Project: Molecular and Cellular Biology of Fanconi s Anemia Instructor in Medicine (Hematology/Oncology), Children s Hospital, Boston Assistant Professor of Microbiology and Pediatrics, University of Virginia, Charlottesville, VA Associate Professor of Microbiology and Pediatrics, University of Virginia, Charlottesville, VA Administrative Positions: Present Director, Comprehensive Hemophilia Clinic Program Board Certification: Massachusetts Full License, 1992 General Pediatric Boards Certification, Pediatric Hematology/Oncology Board Certification, Virginia Full License, 1998 General Pediatrics Board Recertification, Pediatric Hematology/Oncology Board Recertification

2 Professional Honors & Recognition A) International/National/Regional 2005 Commonwealth Health Research Board grant awardee NIH K02 career development award 2000 Concern Foundation Cancer Research Award 2000 ASH Junior Faculty Award 2000 Basil O Connor-March of Dimes Scholar Award 1997 ASH Travel Award 1995 NIH/NHLBI Clinical Investigator Development Award (K08) 1987 Sarnoff Foundation Research Fellowship 1987 Dean s Research Award, John Hopkins School of Medicine 1983 BS, with high honors 1983 American Cancer Society Student Fellowship 1983 Phi Beta Kappa B) University 2003 Department of Pediatrics Scholar Award 1989 Dean s Research Award Grant History: A) Current Grants Agency: Commonwealth Health Research Board Title: HTLV I tax protein sensitization of p53 mutant tumors P.I.: Percent effort: 20% Direct costs per year: $100,000 Total costs for project period: $200,000 Project period: 07/01/ /30/2007 Agency: NIH/NHLBI ID#: R01 Title: Characterization of the Fanconi Anemia Nuclear Protein Complex P.I.: Percent effort: 40% Direct costs per year: $250,000 Total costs for project period: $1,875,000 Project period: 05/30/ /29/2010 Agency: NIH/NHLBI ID#: K02 Title: Salary /career development award P.I.: Percent effort: 40% Direct costs per year: $93,000 Total costs for project period: $479,000 Project period: 07/01/ /30/2007 2

3 Agency: NIH/CDC Comprehensive Hemophilia Center Title: Characterization of the Fanconi Anemia Nuclear Protein Complex PI.: Direct costs per year: $90,000 Total costs for project period: $180,000 Project period: (routinely renewed) B) Past Grants Agency: Children s Medical Center Career Award Title: HTLV I tax sensitization of p53 mutant cells P.I.: Total costs for project period: $70,000 Project period: Agency: American Society of Hematology I.D.# Scholar Award Title: Subnuclear Localization of the Fanconi Anemia Proteins P.I.: Total costs for project period: $120,000 Project period: Agency: Concern Foundation I.D.# Scholar Award Title: Purification of the Fanconi Anemia Protein Complex P.I.: Total costs for project period: $100,000 Project period: Agency: March of Dimes I.D.# Basil O Connor Award Title: Subnuclear Localization of the Fanconi anemia proteins P.I.: Gary Kupfer, Ph.D. Total costs for project period: $50,000 Project period: Lectures, Courses, Web-based Education: Invited Speaker, Gordon Conference Red Cell Meeting, May Invited Speaker, Children s Hospital donors, UVa, June Invited Speaker, Boston University School of Medicine, Department of Genomics and Genetics, Boston, MA, May

4 Invited Speaker, Johns Hopkins School of Medicine, Hematologic Malignancies Seminar Series, Baltimore, MD, May Invited Speaker, Brown School of Medicine, Department of Pediatrics Grand Rounds, Providence, RI, March Invited Speaker, Department of Otorhinolaryngology Grand Rounds, UVA, October Invited Speaker, First International Pediatric Hematology Conference, Schneider Children s Medical Center of Israel, Sackler School of Medicine, Tel Aviv University, Petach Tikvah, Israel, October Invited Seminar, Yale School of Medicine, Department of Pediatrics, Division of Pediatric Hematology-Oncology Grand Rounds, New Haven, CT, September Invited Seminar, Ontario Cancer Institute, University of Toronto, Toronto, ON, Canada, February Invited Seminar, Division of Hematologic Malignancies, Children s Hospital of Philadelphia, University of Pennsylvania, May Oral presentation (Jun Mi), American Society of Hematology, San Diego, CA, December Children s Hospital Research Center, Ohio State University School of Medicine, invited seminar, February Department of Pediatrics Grand Rounds, Eastern Virginia Medical School, Norfolk, VA, December American Society of Hematology, San Diego, CA, December 2003, oral presentation. Fanconi Anemia Research Foundation, Houston, TX, October 2003, oral presentation. Department of Microbiology Seminar Series, University of Virginia, April 2003 Jason Bennette Memorial Lecture, Einstein Medical School, Long Island Jewish Hospital, New York, New York, April Schneider Children s Hospital, Tel Aviv University, Tel Aviv, Israel, December 2002, invited speaker. Rambam Hospital, Technion University, Haifa, Israel, December 2002, invited speaker. Fanconi Anemia Research Foundation, Philadelphia, PA, December 2002, oral presentation Department of Pediatrics Grand Rounds, University of Virginia, Charlottesville, VA, June 4

5 Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah, April 2002, invited seminar speaker. University of Virginia Cancer Center Seminar, March Fanconi Anemia Research Foundation, Portland, Oregon, December 2001, oral presentation. Fanconi Anemia Research Foundation, Amsterdam, Netherlands, October 2000, oral presentation/research update. Fanconi Anemia Research Foundation, New Orleans, December 1999, oral presentation/research update. Biochemistry and Molecular Genetics Seminar Series, University of Virginia, Charlottesville, VA, September 1999 Fanconi Anemia Research Foundation, Denver, CO, December Fanconi Anemia: Paradigm of Genomic Instability, Bioregulation of Radiation Response: Genetic Determinants, Kyoto, Japan, October 1998, invited speaker. FAA and FAC Bind and Form a Nuclear Complex. Oral presentation at the American Society of Hematology meeting, December FAA and FAC Bind and Form a Nuclear Complex. Oral presentation at the American Society of Human Genetics meeting, October The Fanconi Anemia Polypeptide, FAC, Binds to the Cyclin-Dependent Kinase, cdc2. Oral presentation at the American Society of Hematology meeting, December 1996, Orlando, FL. The Fanconi Anemia Polypeptide, FACC, Normalizes p53 Induction in a Transfected Hematopoietic Cell Line. Oral presentation at the American Society of Hematology meeting, December 1995, Seattle, WA. Parent Attitudes Towards HIV+ Children in Daycare and School. Oral presentation at the Ambulatory Pediatrics Association/Society for Pediatric Research Annual Meeting, 1994, Seattle, WA. 5

6 PROFESSIONAL SERVICE Peer Review Groups/Grant Study Sections 2006 Program Project Grant review, NHLAI, NIH Hematopoiesis Study Section, NHLBI, NIH 2005 Clinical Hematology Study Section, NHLBI, NIH 2005 Congressional Directed Medical Research Program Journal Service Ad hoc reviewer for: Journals: Blood Experimental Hematology Cancer Research Mutation Research Human and Molecular Genetics JBC Journal of Pediatric Hematology-Oncology European Journal of Hematology Journal of Cell Biology Molecular and Cell Biology Haematologica/The Hematology Journal British Medical Bulletin EMBO Pediatric Blood and Cancer Genes to Cells Nature Genetics Organizations Fanconi Anemia Research Foundation UVa Summer Undergraduate Research Program Cancer Research Campaign (UK) Imperial Cancer Research Fund (UK) University of Liverpool Cancer Research Fund (UK) Northwest Cancer Research Fund (UK) US-Israel Binational Science Foundation American Society of Hematology (ASH) abstract reviewer for annual meeting, 2005 ASH abstract reviewer and abstract group leader for annual meeting, 2006 Medical Research Charities Group (Ireland) Netherlands Organisation for Scientific Research (Netherlands) Dutch Cancer Society (Netherlands) Professional Organizations American Society of Pediatric Hematology/Oncology American Society of Hematology Virginia Medical Society Society for Pediatric Research 6

7 University of Virginia Service University Committees 2005 Neonatology Division Head Search Committee MD-PhD Executive Committee 2004 Dissertation committee, Jeffrey Blackwell 2004 Comps committee, Huy Ty 2003 Comps committee, Amanda Weaver 2003 Planning Committee, Genomic Instability Symposium 2003 Dissertation Committee, Bjarki Stefansson 2003 Dissertation Committee, Andy High, Department of Chemistry Protocol Review Committee, Cancer Center Dean s Research Planning Committee 2002 Comps committee, Michelle Demory Dissertation committee, Christine Kessler Dissertation committee, Kurt Jensen 2001 Comps committee, Alex Ward Search Committee, Mellon Prostate Cancer Center recruitment 2001 Dissertation Defense Committee, Zhenghe Wang 2000-present Cancer Center member Departmental Committees 2005 Pediatric Hem-Onc Division Head Search Committee 2001 Search Committee, Division of Pediatric Hematology- Oncology, Dept of Pediatrics, clinical faculty recruitment 2001 Brochure Committee, Dept of Microbiology 2000-present Graduate Student Recruitment, Dept of Microbiology Outside Committees May 2006 External PPG reviewer, Oregon Health Sciences University, Portland, OR 2004-present Hodgkin lymphoma, Biology Committee, Children s Oncology Group Spring 2005 Susan Gordan, PhD candidate, University of Toronto Ronald McDonald House Board of Directors BIBLIOGRAPHY: 1. Peer-Reviewed Manuscripts 1. Rowe T, Kupfer G, Ross W. Inhibition of Epidophyllotoxin Cytotoxicity by Interference with Topoisomerase-mediated DNA Cleavage. Biochemical Pharmacology, 34: , Kupfer GM, Bodley A, Liu LF. Involvement of Intracellular ATP in the Cytotoxicity of Topoisomerase II-targeting Antitumor Drugs. NCI Monographs, 4: 37-40,

8 3. Kupfer GM, Giardino AP. Reimbursement and Insurance Coverage in Cases of Suspected Sexual Abuse in the Emergency Department, Child Abuse and Neglect, 19: 291-5, Yamashita T, Wu N, Kupfer G, et.al. The Clinical Variability of Fanconi Anemia (Type C) Results from Expression of an Amino Terminal Truncated FAC Polypeptide with Partial Activity, Blood, 87: , IF 9.6, 40 hits 5. Kupfer GM, D Andrea AD. The Effect of the Fanconi Anemia Polypeptide, FAC, Upon p53 Induction and G2 Checkpoint Regulation, Blood, 88: , IF 9.6, 27 hits 6. Kupfer GM, Yamashita T, Näf D, Suliman A, Asano S, D Andrea AD. The Fanconi Anemia Polypeptide, FAC, Binds to the Cyclin-Dependent Kinase, cdc2, Blood, 90: , IF 9.6, 53 hits 7. Kupfer GM, Näf D, Suliman A, Pulsipher M, D Andrea AD. The FA Proteins, FAA and FAC, Bind Each Other and Form a Nuclear Complex, Nature Genetics, 17: , IF 26.7, 110 hits 8. Pulsipher M, Kupfer GM, Naf D, Suliman A, Sieff C, Mulligan R, D'Andrea A. Functional Correction and Rapid Subtyping of FA Cells Using Psuedotyped Retroviruses. Molecular Medicine, 4, p , IF 3.3, 20 hits 9. Naf D, Kupfer GM, Suliman A, Lambert K, D'Andrea A. Functional Activity of the Fanconi Anemia Protein, FAA, requires FAC Binding and Nuclear Localization. MCB, 18, p , IF 8.8, 58 hits 10. Yamashita T, Kupfer GM, Naf D, Suliman A, Joenje H, Asano S, D Andrea AD. The Fanconi Anemia Pathway requires FAA Phosphorylation and FAA/FAC Nuclear Accumulation. PNAS, 95: , IF 10.7, 61 hits 11. Kupfer GM, Naf D, Garcia-Higuera I, Wasik J, Cheng A, Yamashita T, Tipping A, Morgan N, Mathew CG, D Andrea AD. A Patient-derived Mutant Form of the Fanconi Anemia Protein FANCA is defective in Nuclear Accumulation. Experimental Hematology, 27: , IF 3.4, 14 hits 50% of this work was done by Dr. Kupfer at the University of Virginia. 12. Qiao F, Moss A, Kupfer GM. Fanconi Anemia Proteins Localize to Chromatin and Nuclear Matrix in a DNA damage and cell cycle inducible fashion. JBC, 276: , IF 6.7, 16 hits 13. Al-Hadoui A, Wong E, Kupfer G, Semmes OJ. HTLV I Tax1 protein associates with chk2. JBC, 278, , Qiao F, Mi J, Wilson JB, Moss A, High A, Jones NJ, Kupfer GM. FANCG is phosphorylated at serine 7 which is required for normal Fanconi anemia function. JBC, 279, , Mi J, Qiao F, Wilson JB, High A, Schroeder M, Shabanowitz J, Hunt DF, Jones NJ, Kupfer GM. FANCG is phosphorylated at serine 383 and 387 during mitosis. MCB, 24, ,

9 16. Thomashevski A, High A, Drozd M, Grant P, Hunt D, Kupfer G. The Fanconi anemia core complex exists as 4 different sized complexes in different cellular compartments. JBC, 279, , Mi J, Kupfer GM. Fanconi anemia core complex associates with chromatin during S phase as nuclear foci. BLOOD, 105, , Hussain S,Wilson JB, Blom E, Thompson LH, Sung P, Gordon SM, Kupfer GM, et.al. Tetratricopeptide-motif-mediated interaction of FANCG with recombination proteins XRCC3 and BRCA2. DNA Repair, 5, , Machida YJ, Machida Y, Chen Y, Gurtan AM, Kupfer GM, D Andrea AD, Dutta A. UBE2T is the E2 in the Fanconi Anemia Pathway and Undergoes Negative Regulation byautoubiquitination. Molecular Cell, vol 23, p589-96, Reviews, Chapters, Books 1. Kupfer GM, Näf D, D Andrea AD. The Molecular Biology of Fanconi Anemia, Hematology/Oncology Clinics of North America, 11: , Kupfer GM. Childhood Cancer Epidemiology, in Pediatrics, electronic textbook (via PubMed), Kupfer GM. Congenital DNA Repair Disorders, in DNA Repair in Cancer Therapy, Humana Press, Totowa, New Jersey, Collins NB, Kupfer GM. Molecular Pathogenesis of Fanconi Anemia. International Journal of Hematology, 82, p , , Kupfer GM. Neonatal Bone Marrow Hypoplasia, in Neonatal Hematology, Cambridge University Press, Cambridge, UK, Kupfer GM. FANCD1 and FANCD2, chapter in Molecular Mechanisms of Fanconi anemia, Springer, New York, Kupfer GM. Childhood Cancer Epidemiology, in Pediatrics, electronic textbook (via PubMed), 2006 (updated from 2001). Papers in Press N/A 9