About OMICS Group. OMICS Group International is an amalgamation of Open Access acetyl-coa

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1 About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 27 with the sole aim of making the information on Sciences and technology Open Access, OMICS Group publishes 5 online [ 4-aspartate] open access scholarly journals in all aspects of Science, Engineering, Management and Technology journals. OMICS Group has been instrumental iso in taking the knowledge on Science & technology to the doorsteps of ordinary men and women. Research Scholars, Students, Libraries, 2 Turn Educational Institutions, Research centers and the industry are main stakeholders that benefitted greatly from this knowledge dissemination. OMICS International also organizes 5 International conferences annually across the globe, where knowledge transfer takes place through debates, round table discussions, poster presentations, workshops, symposia and exhibitions.

2 About OMICS Group About OMICS International Conferences OMICS International is a pioneer and leading science event organizer, which publishes around 5 open access journals and conducts over 3 Medical, Clinical, Engineering, Life Sciences, Pharma scientific conferences all over the globe annually with the support of more than 1 scientific associations and 3, editorial board members and 3.5 million followers to [ 4-aspartate] its credit. iso OMICS International has organized 5 conferences, workshops and Vegas, San Antonio, Omaha, Orlando, Raleigh, Santa national symposiums across the major cities including San Francisco, Las Clara, Chicago, Philadelphia, Baltimore, United Kingdom, Valencia, Dubai, Beijing, Hyderabad, Bengaluru and Mumbai.

3 Mass spectrometry-based metabolic profiles of brain cancer [ 4-aspartate] iso KHALID AL-QAHTANI

4 IDH1-R132H signal intensity Number of "Metabolomics" Publications [ 4-aspartate] Publication Year iso 1 2HG 5 2OG 5 1 IDH1-Wild-type signal intensity

5 Time (min) Flow ml/min % Mobil phase A 1mM TBA + 15 AcOH % Mobil phase B ACN +.1% FA initial [ 4-aspartate] iso / isom/z 191 m/z Hydroxyglutarate Glutamic Glutamate / 2-Oxoglutarate m/z 147 m/z 146 m/z 145 Malate Aspartic m/z 133 m/z 132 Itconitate m/z 129 Succinate Fumarate m/z 117 m/z Retention time (min)

6 ACE-C18-PFP ACE-C18-PFP ACE-C18-PFP Kinetex-C18-PFP Kinetex-C18-PFP acquity-hsst3 acquity-hsst3 Kinetex-C18-PFP ACE-C18-PFP ACE-C18-PFP Kinetex-C18-PFP Kinetex-C18-PFP acquity-hsst3 acquity-hsst3 acquity-hsst3 Intensity (b1) iso 2 1 (a1) iso Intensity 4 (a2) 3 2 maleate (b2) maleate Intensity (a) Glu Gln Asp ICA Mat 2-HG SA ACT FUM CA 2-OG 4 (c1) iso Time (min) (c2) maleate Time (min) (b) 2-HG ICA Asp Mat FUM ACT CA Intensity (a2) [ 4-aspartate] Intensity 15 (a1) Glu SA Gln 2-OG OG iso (b2) 3 2 (b1) (c) 2-OG FUM CA ACT 1 st 2-HG Turn ICA SA MAT iso (c1) (c2) 2OG 2OG Gln Glu Asp Time (min) Time (min) Time (min)

7 1 [ 4-aspartate] 8 iso 6 4 2

8 Response Standard Addition Plot 6 y = 3.978x R² =.997 The x-intercept is [ 4-aspartate] Concentration [µm] of Added Analyte iso

9 1H MRS measures of 2-HG (µmoles/g) R² =.64 [ 4-aspartate] 6 iso Mass spectroscopy measures of 2-HG (µmoles/g)

10 [ 4-aspartate] iso

11 concentration ( µm) Limit of detection 5μM 25 Limit of quantification calibration curve linearity [ 4-aspartate] 1μM beyond 2 μm 2 15 Intra-day precision (n=3) inter-day precision (n=3) 5.8% 13.6% iso 1 5 wild type Mutant standard deviation (SD) 7.3

12 Peak Area [ 4-aspartate] iso peak area vs Incubation time Succinate Citrate Glutamine Oxaloacetate incubation time (min)

13 Concentration (μm/ Cells) Concentration (μm/4.66 Cells) 1 CA ICA CCA IA 2OG OAA ASP Mal Gln Glu [ 4-aspartate] iso 1 1 R132H - Basal R132H + L-Asp R132H + D-Asp 1 1

14 [ 4-aspartate] iso

15 [ 4-aspartate] [ 4-aspartate] 13 iso iso

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17 Let Us Meet Again We welcome you all to our future conferences of OMICS International [ 4-aspartate] Please Visit: iso + NAD