Faculty of Natural Science, University of Salzburg, Austria
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1 First International Meeting of the HNE-Club* 4-Hydroxynonenal and other Lipid Peroxidation Products Programme / Abstracts Conference plus Practical Course Faculty of Natural Science, University of Salzburg, Austria July 13-15, 2002 * Group of interest of the International Society for Free Radical Research A satellite meeting of the Biannual Congress of the ISFRR 1
2 Table of Content 1. PROGRAMME 1.1. CONFERENCE SATURDAY SUNDAY WORHSHOP - MONDAY ABSTRACTS 2.1. LECTURES WORKSHOP-LECTURES ORAL COMMUNICATIONS OF SELECTED POSTERS POSTERS Biological Effects Biochemical Reactions Signalling Gene Expression Metabolism Marker of Oxidative Stress SUPPLEMENT AUTHORS INDEX
3 First International Meeting of the HNE-Club 4-Hydroxynonenal and other Lipid Peroxidation Products Salzburg, Austria July 13-15, 2002 PROGRAMME 3
4 ACKNOWLEDGEMENT The Scientific Programme Commitee and the Local Organizing Committee wish to thank the following organizations for their financial support SPONSORS Neuroscience Foundation Cavaliere Ottolenghi Tecan Sales International GmbH County of Salzburg University of Salzburg Dr. Franz Tazber KEG Fresenius Kabi Austria GmbH Sarstdt AG & Co Ökopharm GmbH 4
5 SCIENTIFIC COMMITTEE P. M. ECKL Institute of Genetics and General Biology, University of Salzburg, Austria, G. POLI Department of Clinical and Biological Sciences, University of Turin, Italy. Coordinator of the HNE-Club R. J. SCHAUR Institute of Molecular Biology, Biochemistry & Microbiology, University of Graz, Austria Secretary of the HNE-Club N. ZARKOVIC Department of Molecular Medicine, Institute of Rudjer Boskovic, Zagreb, Croatia LOCAL ORGANIZING COMMITEE S. AITZETMÜLLER Department of Research Promotion University of Salzburg, Austria, P. ECKL/ N. BRESGEN Institute of Genetics and General Biology, University of Salzburg, Austria, M. SCHLAGER Institute of Genetics and General Biology, University of Salzburg, Austria, 5
6 REGISTRATION AND INFORMATION The registration and information desk will be open in the entrance area of the Auditorium Maximum MEALS Lunch will be available in the Mensa of the Faculty of Natural Sciences. ORAL PRESENTATIONS Speakers should hand their slides to the projectionist at least half an hour before the beginning of each session. Overhead projection and Videobeamer presentation will be also available. POSTERS All posters should be mounted on the poster boards on Saturday and removed on Sunday afternoon. LOCAL TRANSPORTATION From train station: Bus-No. 51 direction Alpensiedlung Süd, leave at bus stop Faistauer Gasse / Landesregierung, 5 min. walk to NAWI. From airport: Bus-No. 77 direction train station. Take bus no. 51, leave at bus stop Faistauer Gasse / Landesregierung, 5 min. walk to NAWI. By car: Motorhighway (A1) exit Salzburg Süd direction Salzburg Zentrum, Alpenstraße to Michael-Pacher- Straße. Parking is possible in the parking house of the NAWI. Walk: You can walk within half an hour from the center of Salzburg to the Faculty of Natural Sciences Faculty of Natural Sciences 6
7 Saturday, July 13th 08:00-09:00 9:00 Registration and Information Opening Remarks (Auditorium Maximum) Eckl P. (Chairman of the organizing Committee) Poli G. (Coordinator of the International HNE-Club) Boveris A. (President of the International Society for Free Radical Research) 1st Session: (Auditorium Maximum) Chair: Boveris A (Buenos Aires, Argentina) 9:30 10:00 Schaur R.J.: (Graz, Austria) Eckl P.: (Salzburg, Austria) Basic Aspects of the Biochemical Reactivity of 4-Hydroxynonenal Genotoxicity of 4-Hydroxynonenal 10:30 Siems W.: (Bad Harzburg, Germany) Metabolism of 4-Hydroxynonenal Coffe break 11:00-11:30 2nd Session: (Auditorium Maximum) Chair: Azzi A. (Bern, Switzerland) 11:30 Forman H.: (Birmingham, USA) HNE: Signaling Pathways Leading to its Elimination 7
8 12:00 12:30 Marinari U.M.: (Genova, Italy) Uchida K.: (Nagoya, Japan) Role of PKC-Dependent Pathways in HNE-induced Cell Protein Transport and Secretion Acrolein: the Product and Mediator of Oxidative Stress Lunch Buffet + Poster Viewing 13:00-15:00 3rd Session: (Auditorium Maximum) Chairs: Sevanian A. (Los Angeles, USA), Fraga C. (Buenos Aires, Argentinia) 15:00-17:30 Oral Communications 01. Aldini G.: Characterization of HNE-Carnosine Reaction Products by ESI-MS/MS and Detection by LC-MS in Rat Skeletal Muscle 02. Cravedi J.P.: Enantioselective Metabolism of (R)- and (S)- 4-Hydroxynonenal in Rat 03. Srivastava S.K.: Involvement of Aldose Reductase in the Metabolism of 4-Hydroxynonenal and its Glutathione Conjugate: Kinetic and Structural Studies 04. Bauer H.C.: Oxidative Stress in Cultured Cerebral Endothelial Cells induces Chromosomal Aberrations; Micronuclei and Apoptosis 05. Masotti L.: Cytotoxic and Cytostatic Effects induced ba 4-Hydroxynonenal in Human Osteosarcoma Cells
9 06. Henderson G.I.: HNE Mediates Apoptotic Cell Death in the Developing Brain Exposed to Ethanol 07. Spickett C.: Comparison of the Cytotoxicity of Phospholipid Chlorohydrins and Aldehydes in Cultured Mammalian Cells 08. Zarkovic K.: Immunohistochemical Appearance of HNE-Protein Conjugates in Human Astrocytoma 09. Awasthi Y.: 4-Hydroxynonenal and Stress Mediated Signaling 10. Nakashima I.: 4-Hydroxynonenal Triggers A Novel Signal Transduction Cascade for a Positive Feedback Regulation of Caspase Activation 11. Leonarduzzi G.: UP-Regulation of TGFbeta1 in Macrophages by 9-Oxononanoyl Cholesterol 12. Leitinger N.: Oxidized Cholesteryl Linoleates Stimulate Endothelial Cells to bind Monocytes via Extracellular Signal Regulated Kinase 1/2 Pathway 13. Loidl A.: Biologically Active Phospholipids and Ceramide Role in mmldl-induced Interacellular Signaling of Smooth Muscle Cells 14. Schäferhenrich A.: 4-Hydroxynonenal Induce Damage of TP53 in Human Colons Cells Chair: Davies K.J.A. (Los Angeles, USA) 4th Session: (in the Aula of the University) 19:00 Hermann-Esterbauer-Award Ceremony 19:15 Dianzani M.U.: (Turin, Italy) Key-Note Lecture: HNE: from Pathology to Physiology 9
10 Sunday, July 14th 5th Session: (Auditorim Maximum) Chair: Niki E. (Ikeda, Japan) 9:00 Davies K.J.A. (Los Angeles, USA) HNE, Protein Aggregates and Proteasome 09:30 10:00 10:30 Vina J.: (Valencia, Spain) Zarkovic N.: (Zagreb, Croatia) Grune T.: (Berlin, Germany) Relationship between Malondialdehyde Formation and Glutathione Oxidation in Hepatic Aging and Disease HNE as a Marker of Pathophysiological Processes Degradation of HNE-Modified Proteins Coffe break 11:00-11:30 6th Session: (Auditorium Maximum) Chairs: Cadenas E. (Los Angeles, USA), Basaga H. (Istanbul, Turkey) 11:30-13:00 Oral Communications 15. Bhatnagar A.: Role of 4-Hydroxynonenal in Myocardial Ischemic injury and Adapation 16. Chen J.: HNE as a Mediator of Oxidative Stress- Associated Mitochondrial Damage in Rat Heart and Liver 10
11 17. Cutrin J.C.: Ischemic Preconditioning Attenuates the Oxidant-Dependent Mechanisms of Reperfusion Cell Damage and Death in Rat Liver 18. Montilla-López P.: Brain Oxidative Stress induces by the CentralIinhibition of the Serotonin Synthesis 19. Borovic S.: HNE and Transferrin Receptors of Cells of Colorectal Carcinoma and CaCo2 Cells Treated with Iron Compound 20. Zanetti D.: 4-Hydroxnonenal and transforming Growth Factor beta1 Expression in Colon Cancer 21. Verde V.: Oxidative Status in Chronic Hepatitis C. During Interferon and Ribavirin Combined Therapy 22. Resch U.: Lipid Peroxidation in Thyroid Dysfunction Lunch Buffet + Poster Viewing 13:00-14:30 11
12 7th Session: (Auditorim Maximum) Chair: Vina J. (Valencia, Spain) 14:30 15:00 15:30 Poli G. (Turin, Italy) Negre-Salvayre A. (Toulouse, France) Smith M. (Cleveland, USA) Apoptotic and Fibrogenic Effects of Oxysterols Oxidized LDL and 4-Hydroxynonenal Modulate Tyrosine Kinase Receptor Activity Phosphorylation Regulation of HNE-Adduction to Proteins: Relevance to Neurodegenerative Disease Social Programme 16:30 Guided sightseeing Tour: Burg Hohensalzburg 19:30 Congress - Dinner at the Restaurant Stiegl Keller 12
13 Monday, July 15th - Workshop 1. Lectures: (Seminarroom Genetic ) 9:00 9:30 10:00 10:30 Mittelbach M. : (Graz, Austria) Waeg G.: (Graz, Austria) Zarkovic K.: (Zargreb, Croatia) Eckl P./ Bresgen N.: (Salzburg, Austria) Synthesis and Characterization of HNE and Chlolesteryl Core Aldehydes Applications of HNE-Specific Antibodies Detection of HNE in the Central Nervous Systems Determination of Genotoxicity of HNE During the lectures an experiment can be observed via a monitor (treatment of HeLa cells by 100 micromolar HNE 2 Practical Course: (biochemistry lab, microscopy lab) 3 groups of experiments in parallel lasting for about 1.5 hours each Borovic S.: (Zagreb, Croatia) F. Biasi (Turin, Italy) Eckl P./ Bresgen N. (Salzburg, Austria) Preparation of free HNE from acetal derivative and HPLC of (free or hydrazone derivative) HNE Sister Chromarid Exchanges, Chromosomal Aberrations and Micronuclei caused by HNE (double staining for HNE also) Zarkovic K. / Immunohistochemistry / Immunocytochemistry / Loncaric I./ Cipak A. Western blot / dot blot of HNE conjugates (Zagreb, Croatia) 12:30-13:00 Lunch will be taken in the lab After the experiments the immunofluorescence of HNE-treated HeLa cells will 13
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