Effective Surface Area (m 2 /g) Pore Size (Å)
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1 n e w Ultra-High Performance on Any LC System Achieve sub- µm performance within HPLC backpressure limitations Substitute µm and µm columns for -x higher efficiency Obtain higher throughput without sacrificing resolution Easy method transfer across LC system platforms Reduce solvent consumption with faster analysis Reach lower levels of detection and quantitation Material Characteristics* Total Particle Size (µm) Porous Shell (µm) Solid Core (µm) Packing ph Material Stability Kinetex XB-C ** Pore Size (Å) Effective Surface Area (m /g) Effective Carbon Load % Kinetex C ** Kinetex C Kinetex PFP Kinetex HILIC Kinetex XB-C ** Kinetex C ** Kinetex C Kinetex PFP Kinetex HILIC *For the evaluation of effective surface area and carbon load, please request technical note, TN- **Columns are ph stable from.- under isocratic conditions. Columns are ph stable - under gradient conditions.. mm ID Kinetex columns are pressure stable up to bar. Pressure Stability / bar bar If you are not completely satisfied with Kinetex core-shell columns, send in your comparative data to a similar product within days and KEEP THE COLUMN FOR FREE. R&D Award Recipient Innovation in Particle Technology The Kinetex core-shell particle is not fully porous. Using sol-gel processing techniques that incorporate nano structuring technology, a durable, homogenous porous shell is grown on a solid silica core. This highly optimized process combined with uniform particle size distribution produces a column that generates extremely high plate counts. When using Kinetex. µm, less column backpressure is generated, allowing it to be used on any LC system. Kinetex. µm Core-Shell Particle Reduced diffusion path maximizes efficiency Increased efficiencies compared to traditional fully porous sub- µm columns. Typical operating backpressures > bar Kinetex. µm Core-Shell Particle Reduced diffusion path maximizes efficiency Ultra-high performance on any system with Kinetex. µm columns. µm Solid Core. µm Porous Shell. µm Solid Core. µm Porous Shell. µm. µm SEM of Kinetex Core-Shell Particles Cross Section of Kinetex Core-Shell Particle When using Kinetex. µm, increased performance can be achieved, however higher pressure-capable instrumentation is required. Phenomenex
2 Optimized for Ultra-High Performance Faster Mass Transfer Since the Kinetex particle is not fully porous, analytes spend less time diffusing into and out of the pores as they travel through the column. This shorter diffusion path allows for faster mass transfer. The result is less band broadening for higher peak efficiency comparable to or better than sub- µm fully porous particles. High Efficiency over Extended Range of Flow Rates. µm Kinetex Core-Shell Sub- µm Fully Porous Band Broadening Band Broadening HETP (µm) Double the efficiency of µm at high speed Sub- µm exceeds conventional pressure here µm µm Fully Porous Linear Velocity (mm/s) Kinetex. µm Core-Shell Sub- µm Fully Porous µm Fully Porous Band Broadening HPLC kinetex Kinetex particles are nearly monodispersed. This extremely narrow particle size distribution results in ultra-high column efficiency and excellent reproducibility. Uniform Particle Size Distribution Particle Count Particle Diameter (µm) Kinetex. µm Core-Shell Traditional Fully Porous. µm Traditional Fully Porous µm Batch-to-Batch Reproducibility Overlay Conditions same for all batches: Column: Kinetex. µm C Dimensions: x. mm Part No.: B--E Mobile Phase: Water / Acetonitrile (:) Flow Rate:. ml/min Detection: nm. Uracil. Hydroxycortisone. Cortisone. Cortisone acetate. -Hydroxyprogesterone Batch R s (,). Batch R s (,). Batch R s (,). min Batch Batch Batch App ID,, Phenomenex
3 Achieve Sub- µm Performance within HPLC Backpressure Limitations With the efficiency of a sub- µm column and typical operating backpressure less than bar, you can achieve the promise of ultra-high performance on any LC system. Traditional. µm N =, p/m > bar! Method cannot transfer to a non-uhplc instrument Kinetex. µm N =, p/m < bar! Easier method transfer to ANY system min Unparalleled Levels of Ultra-High Performance For users of higher pressure capable instruments who want the highest level of efficiency, we introduce the Kinetex. µm column - the first sub- µm core-shell particle available on the market. Traditional. µm N =, p/m min Kinetex. µm N =, p/m App ID App ID App ID App ID Column: Kinetex. µm C Traditional. µm C Dimensions: x. mm Mobile Phase: Acetonitrile / Water (:) Flow Rate:. ml/min Temperature: C Detection: nm Instrument: *Waters ACQUITY UPLC. µl test mixture. Acetophenone. Benzene. Toluene. Naphthalene min min Column: Kinetex. µm C Traditional. µm C Dimensions: x. mm Mobile Phase: Acetonitrile / Water (:) Flow Rate:. ml/min Temperature: C Detection: nm Instrument: *Waters ACQUITY UPLC. Acetophenone. Benzene. Toluene. Naphthalene Kinetex. µm columns,. mm ID, are pressure rated to bar use on both HPLC and UHPLC instrumentation. * Waters, ACQUITY, and UPLC are registered trademarks of Waters Corporation. Phenomenex is not affiliated with Waters Corporation. Comparative separations may not be representative of all applications. Phenomenex
4 Substitute µm and µm Columns for -x Higher Efficiency Replace traditional µm or µm analytical columns with Kinetex. µm core-shell columns for immediate performance improvements in efficiency, speed, resolution, and sensitivity. Optimize methods for ultra-high performance and transfer them to any system. Traditional µm N =, p/m Kinetex. µm N =, p/m Column: Kinetex. µm C Traditional µm C Dimensions: x. mm Mobile Phase: Acetonitrile / Water (:) Flow Rate:. ml/min Temperature: C Backpressure: bar (Kinetex) bar (Traditional µm) Detection: nm Instrument: Agilent SL App ID min. Uracil. Acetophenone. Toluene. Naphthalene Obtain Higher Throughput without Sacrificing Resolution min For the ultimate sample throughput demands, Kinetex columns provide the efficiency needed to significantly reduce run times. In this separation of ketones, a -fold increase in productivity is accomplished while still maintaining resolution. Traditional µm > min Rs, =. App ID < min Resolution maintained Rs, =. App ID Kinetex. µm App ID HPLC kinetex Column: Traditional µm C Dimensions: x. mm B: Acetonitrile Gradient: Time (min) % B.. Flow Rate:. ml/min Temperature: C Detection: nm min. Acetone. -Butanone. -Pentanone. Acetophenone. -Heptanone. Butyrophenone. -Nonanone Time (min) % B... Hexanophenone. Octanophenone. -Tridecanone. Decanophenone. -Pentadecanone. -Hexadecanone Comparative separations may not be representative of all applications. min Column: Kinetex. µm C Dimensions: x. mm Part No.: B--E B: Acetonitrile Gradient: Time (min) % B.. Flow Rate:. ml/min Temperature: C Detection: nm Backpressure: bar. Acetone. -Butanone. -Pentanone. Acetophenone. -Heptanone. Butyrophenone. -Nonanone Time (min) % B.... Hexanophenone. Octanophenone. -Tridecanone. Decanophenone. -Pentadecanone. -Hexadecanone Phenomenex
5 Easy Method Transfer Across LC System Platforms UHPLC methods developed with fully porous sub- µm columns often generate backpressure higher than HPLC system limitations. With Kinetex. µm core-shell technology, you are no longer restricted from developing high performance LC methods on any system and transferring them anywhere. In these examples different internal diameters of Kinetex columns are used on various systems to illustrate the versatility of Kinetex core-shell technology. Please note the flow rates are scaled to maintain the same linear velocity. Kinetex. mm ID on Agilent Column: Kinetex. µm C Dimensions: x. mm Part No.: B--E Mobile Phase: Acetonitrile / Water (:) Flow Rate:. ml/min Temperature: Ambient Detection: nm. Uracil. Acetophenone. Toluene. Naphthalene Kinetex. mm ID on *Shimadzu Prominence UFLCx r Column: Kinetex. µm C Dimensions: x. mm Part No.: B--Y Mobile Phase: Acetonitrile / Water (:) Flow Rate:. ml/min Temperature: Ambient Detection: nm. Uracil. Acetophenone. Toluene. Naphthalene App ID App ID min min Kinetex. mm ID on Agilent SL Kinetex. mm ID on *Waters ACQUITY UPLC Column: Kinetex. µm C Dimensions: x. mm Part No.: B--AN Mobile Phase: Acetonitrile / Water (:) Flow Rate:. ml/min Temperature: Ambient Detection: nm. Uracil. Acetophenone. Toluene. Naphthalene Column: Kinetex. µm C Dimensions: x. mm Part No.: B--AN Mobile Phase: Acetonitrile / Water (:) Flow Rate:. ml/min Temperature: Ambient Detection: nm. Uracil. Acetophenone. Toluene. Naphthalene App ID App ID min min * Waters, ACQUITY, and UPLC are registered trademarks of Waters Corporation. Prominence and UFLC are trademarks of Shimadzu Corporation. Phenomenex is not affiliated with Agilent Technologies, Waters Corp. or Shimadzu. Phenomenex
6 Improve Performance, Save Solvent When chromatographic column performance improves you can not only decrease your analysis time but also decrease your overall solvent consumption without compromising your separations. Use Kinetex core-shell technology to dramatically decrease the solvent consumption in your laboratory and increase sample throughput. Column: Traditional µm C Dimensions: x. mm Flow Rate:. ml/min Example Method Consumption ml solvent per run! App ID min Column: Kinetex. µm C Dimensions: x. mm Part No.: B--AN Flow Rate:. ml/min Less Solvent Consumption with Kinetex Column < ml solvent per run! Mobile Phase: A: mm Potassium phosphate ph B: Methanol / Acetonitrile (:) A/B (:) Temperature: ºC Detection: nm min App ID. Tianeptine. Desmethyldoxepin. Protriptyline. Desipramine. Imipramine. Amoxapine. Doxepin. Nortriptyline. Amitriptyline. Clomipramine HPLC kinetex Reach Lower Levels of Detection and Quantitation The combination of the small particle size, narrow particle size distribution, and the significantly shorter diffusion path results in much higher column efficiencies and increased chromatographic resolution. The increased efficiencies provide an immediate benefit on sensitivity since higher chromatographic efficiencies translate into significantly narrower and taller peaks, making it easier to detect low level impurities. *ZORBAX. µm SB-C Kinetex. µm C App ID.. App ID min min Dimensions: x. mm B: Acetonitrile Gradient: (:) A/B for. min, then to (:) A/B Flow Rate:. ml/min Temperature: ºC Detection: nm Instrument: Agilent. Pyridine. Acetaminophen. Pindolol. Quinine. Acebutolol. Chlorpheniramine. Triprolidine. Prednisolone. Nortriptyline. -Chlorobenzoic acid. -Methyl--hydroxy benzaldehyde. -Chlorocinnamic acid. Diazepam. Diflunisal. Niflumic acid. Hexanophenone * Zorbax is a registered trademark of Agilent Technologies. Comparative separations may not be representative of all applications. Phenomenex is not affiliated with Agilent Technologies. Phenomenex
7 Complementary and Orthogonal Selectivities To provide alternative and orthogonal selectivity phases, Kinetex columns are available in selectivities: XB-C, C, C, PFP (Pentafluorophenyl), and HILIC (Hydrophilic Interaction Liquid Chromatography), for resolution of a wide range of compounds from polar to hydrophobic, aromatic, and isomers. NEW! Kinetex XB-C Protective butyl side-chains allow superior peak shape and enhanced separation of basic compounds Kinetex C Offers the hydrophobic retention and methylene selectivity chromatographers expect from a C column. Low High Low High Si Hydrophobicity: Hydrophobicity: Polarity: Polarity: XB- C H-Bonding: Aromatic Selectivity: Silanol Activity: ph Range:. * USP Classification: L Particle Sizes:. µm &. µm Effective Carbon Load: % C H-Bonding: Aromatic Selectivity: Silanol Activity: ph Range:. * USP Classification: L Particle Sizes:. µm &. µm Effective Carbon Load: % NEW! Kinetex C Brings the benefits of core-shell technology to USP L and other C column methods. Kinetex PFP F F F Pentafluorophenyl phase incorporates fluorine atoms on the periphery of the phenyl ring to provide unique aromatic and polar selectivity. Hydrophobicity: Low High F F Hydrophobicity: Low High C Polarity: H-Bonding: Aromatic Selectivity: Silanol Activity: PFP Polarity: H-Bonding: Aromatic Selectivity: Silanol Activity: ph Range:. * USP Classification: L Particle Sizes:. µm &. µm Effective Carbon Load: % ph Range:. USP Classification: L Particle Sizes:. µm &. µm Effective Carbon Load: % Kinetex HILIC H ACN ACN ACN ACN ACN ACN ACN ACN ACN ACN ACN ACN H ACN ACN ACN ACN Unbonded silica phase used under hydrophilic interaction chromatography conditions to provide selectivity for the retention and separation of polar compounds. H HO HO O H OH H H O H HO H Si H OH Si HILIC Si OH Hydrophobicity: Polarity: H-Bonding: Aromatic Selectivity: Silanol Activity: Low ph Range:.. USP Classification: L Particle Sizes:. µm &. µm Carbon Load: High *Columns are ph stable from.- under isocratic conditions. Columns are ph stable - under gradient conditions. Phenomenex
8 Veterinary Drugs Steroids C XB-C App ID App ID min min, C App ID C App ID min min Column: Kinetex. µm C Kinetex. µm C Dimensions: x. mm Mobile Phase: A:.% Phosphoric acid in Water B:.% Phosporic acid in Acetonitrile Gradient: Time (min) % B. Flow Rate:. ml/min Temperature: ºC Detection: nm. Naproxen. Fenoprofen. Ibuprofen. Phenylbutazone Time (min) % B. Indomethacin. Meclofenamic acid. Matrix impurity. Matrix impurity Column: Kinetex. µm XB-C Kinetex. µm C Dimensions: x. mm B: Acetonitrile Gradient: Time (min) % B Flow Rate:. ml/min Temperature: ºC Detection: nm. Estriol. Hydrocortisone. Cortisone. Estradiol. Cortisone--acetate Time (min) % B.. -Hydroxyprogesterone. Estrone. -Hydroxyprogesterone. Deoxycorticosterone acetate HPLC kinetex Norepinephrine and Epinephrine HILIC Explosives PFP Resorcinol C App ID mi C App ID min App ID C App ID..., App ID App ID min PFP min min... min Column: Kinetex. µm HILIC Kinetex. µm C Dimensions: x. mm Mobile Phase (HILIC): Acetonitrile / mm Ammonium formate ph. (:) Mobile Phase (C): mm Ammonium formate ph. / Methanol (:) Flow Rate:. ml/min Temperature: C Detection: nm. Norepinephrine. Epinephrine Column: Kinetex. µm PFP Kinetex. µm C Dimensions: x.mm B: Methanol Gradient: (:) A/B to (:) A/B over min Flow Rate:. ml/min Temperature: ºC Detection: nm. HMX. RDX.,,-Trinitrobenzene.,-Dinitrobenzene. Nitrobenzene.,,-Trinitrotoluene.,-Dinitrotoluene Column: Kinetex. µm PFP Kinetex. µm C Dimensions: x. mm Mobile Phase:. % Formic acid in Water /. % Formic acid in Acetonitrile (:) Flow Rate:. ml/min Temperature: C Detection: nm. Resorcinol. -Methylresorcinol. Catechol.,-Dimethylresorcinol. -Methylcatechol. -Nitrocatechol Comparative separations may not be representative of all applications. Phenomenex
9 Applications Food Testing Melamine and Cyanuric Acid Column: Kinetex. µm HILIC Dimensions: x. mm Part No.: B--AN Mobile Phase: Acetonitrile / mm Ammonium acetate, ph. (:) Flow Rate:. ml/min Temperature: ºC Detection: *API MS Backpressure: bar Instrument: Waters ACQUITY UPLC MS/MS Sample :. Cyanuric acid -. (quant ion),.-. (qualifier ion). Cyanuric acid-c ISTD.-.. Melamine.- (quant ion),.- (qualifier ion). Melamine-C.N ISTD.-..e,, Azo Dyes Column: Kinetex. µm C Dimensions: x. mm Part No.: F--E Mobile Phase: A:.% Phosphoric acid in Water B:.% Phosporic acid in Acetonitrile Gradient: Time (min) % B Flow Rate:. ml/min Temperature: ºC Detection: nm Backpressure: bar. Orange II. Sudan Orange G. Fast Garnet GBC. Dimethyl yellow. Sudan Red G Time (min) % B.. Sudan I. Sudan II. Sudan III. Sudan IV ESI - ESI +.e.e.e Environmental.... min Carbamate Pesticides: EPA Method. Column: Kinetex. µm C Dimensions: x. mm Part No.: B--AN Mobile Phase: A:. % Phosphoric acid in Water B:. % Phosphoric acid in Acetonitrile Gradient: (:) A/B to (:) A/B over min Flow Rate:. ml/min Temperature: ºC Detection: nm Sample :. Aldicarb sulfoxide. Oxamyl. Aldicarb sulfone. Methomyl. -OH-Carbofuran. Aldicarb sulfonerelated impurity. Aldicarb. Baygon (Propoxur). Carbofuran. Carbaryl. -Napthol. Methiocarb App ID Polyaromatic Hydrocarbons (PAHs): EPA Method Column: Kinetex. µm C Dimensions: x. mm Part No.: D--E B: Acetonitrile Gradient: (:) A/B to (:) A/B over min Flow Rate:. ml/min Temperature: C Detection: nm. Naphthalene. Acenaphthylene. Fluorene. Acenaphthene. Phenanthrene. Anthracene. Fluoranthene. Pyrene min. Chrysene. Benz[a]anthracene. Benzo[b]fluoranthene. Benzo[k]fluoranthene. Benzo[a]pyrene. Dibenz[a,h]anthracene. Indeno[,,-cd]pyrene. Benzo[g,h,i]perylene App ID App ID App ID min min * API is a trademark of Life Technologies Corporation and its affiliated companies. Waters, ACQUITY, and UPLC are registered trademarks of Waters Corporation. Phenomenex is not affiliated with Life Technologies Corporation or Waters Corporation. Phenomenex
10 Applications Biopharmaceutical Peptide Map of Biogeneric a-interferon (unreduced) Column: Kinetex. µm C Dimensions: x. mm Part No.: F--E Mobile Phase: A:. % Trifluoroacetic acid / % Acetonitrile / Water B:. % Trifluoroacetic acid / % Acetonitrile / Water Gradient: Time (min) % B Flow Rate:. ml/min Temperature: ºC Detection: nm Human a-interferon tryptic digest Peptide Map of Reduced and Alkylated Ig-G Column: Kinetex. µm C Dimensions: x. mm Part No.: F--E Mobile Phase: A:. % Trifluoroacetic acid / % Acetonitrile / Water B:. % Trifluoroacetic acid in Acetonitrile Gradient: Time (min) % B Flow Rate:. ml/min Temperature: ºC Detection: nm Hu-Ig-G Reduced and Alkylated Tryptic Digest Pharmaceutical Atenonol Impurity Profile Column: Kinetex. µm C Dimensions: x. mm Part No.: D--E Mobile Phase:. mm Phosphoric acid in Water, ph. +. g Sodium Octanesulfonate +. g Tetrabutyl Ammonium Hydrogen Sulfate / Methanol / THF (::) Flow Rate:. ml/min Temperature: ºC Detection: nm. Impurity B. Impurity D and E. Impurity A. Impurity F. Impurity J. Impurity G. Impurity I. Impurity H App ID min min Forced Degradation of Ranitidine Column: Kinetex. µm C Dimensions: x. mm Part No.: F--E Mobile Phase: A:. % Formic acid in Water B:. % Formic acid in Acetonitrile Gradient: % to % B in min % to % in min Flow Rate:. ml/min Temperature: ºC Detection: nm ( ºC) Ranitidine mg/ml in Methanol. Heated at ºC for days. App ID HPLC kinetex App ID Degradation Products Degradation Products App ID min min Phenomenex
11 If you are not completely satisfied with Kinetex core-shell columns, send in your comparative data to a similar product within days and KEEP THE COLUMN FOR FREE. n e w Ordering Information SecurityGuard. μm Analytical Columns (mm) Ultra Cartridges Phases x. x. x. x. x.. mm ID /pk XB-C B--E C--E D--E F--E AJ- C A--E B--E C--E D--E F--E AJ- C B--E C--E D--E F--E AJ- PFP A--E B--E C--E D--E F--E AJ- HILIC B--E C--E D--E F--E AJ- for. mm ID SecurityGuard. μm Solvent Saver MidBore Columns (mm) Ultra Cartridges Phases x. x. x. x. x.. mm ID /pk XB-C B--Y D--Y AJ- C A--Y B--Y C--Y D--Y F--Y AJ- C B--Y D--Y AJ- PFP A--Y B--Y C--Y D--Y F--Y AJ- HILIC F--Y AJ- for. mm ID n e w SecurityGuard. μm Minibore Columns (mm) Ultra Cartridges Phases x. x. x. x.. mm ID /pk XB-C B--AN D--AN AJ- C A--AN B--AN D--AN F--AN AJ- C B--AN D--AN AJ- PFP A--AN B--AN D--AN F--AN AJ- HILIC B--AN D--AN F--AN AJ- for. mm ID SecurityGuard. μm Minibore Columns (mm) Ultra Cartridges Phases x. x. x.. mm ID /pk XB-C B--AN D--AN AJ- C B--AN D--AN F--AN AJ- C B--AN D--AN AJ- PFP B--AN D--AN F--AN AJ- HILIC B--AN AJ- for. mm ID SecurityGuard Ultra Cartridge System The SecurityGuard Ultra patent pending cartridge system protects ultra-high performance columns, like Kinetex, from damaging contaminants and microparticulates. Extend Kinetex column lifetime Simple to use Pressure rated to, psi (, bar) Fits virtually all manufacturers columns New High Pressure Rated Format SecurityGuard Ultra cartridges require holder, Part No.: AJ- SecurityGuard Ultra Cartridge Holders Ordering Information Part No. Description Unit Price AJ-- SecurityGuard Ultra Cartridge Holder ea AJ- SecurityGuard Ultra Cartridge Holders /pk UHPLC / HPLC Sure-Lok High Pressure PEEK Male Nut Fittings Ordering Information Part No. Description Unit Price AQ- Sure-Lok High Pressure PEEK -Pc Nut -, /pk for / in. Tubing,, psi ( bar) AQ- Sure-Lok Fitting Tightening Tool, Aluminum ea See p. for more information. AQ- AQ- For a convenient, disposable in-line filter that fits virtually all UHPLC / HPLC columns. to. mm ID, use KrudKatcher Ultra, found on p.. For Ultra-High Performance Stainless Steel Nut and Ferrule Set, see p. For Column Heaters, see p. Phenomenex
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