COMBO ULTIMATE HPLC/UHPLC. Kinetex + Luna Omega NEW. Gain Incredible Performance, Versatile Selectivities and Upgrade Throughput

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1 ULTIMATE HPLC/UHPLC CMB Kinetex Luna mega Gain Incredible Performance, Versatile Selectivities and Upgrade Throughput NEW MEGA Core-Shell Technology Luna mega Luna mega PS Kinetex. Kinetex

2 Luna mega Cutting Edge ully Porous Silica Particle Luna is one of the most recognized HPLC brands on the market, delivering high efficiency, ruggedness, reproducibility, and dependability for a wide range of analyses. The new Luna mega builds upon this legacy with an innovative yet rugged silica particle architecture, designed and manufactured by Phenomenex based on more than years of applied knowledge, invention, and customer experience. Novel Design and Manufacturing Process Within the novel manufacturing process of Luna mega silica, we implement a proprietary processing technique to gain greater particle inertness, a stronger particle morphology, and more consistent porosity. UHP HP PREP S Micropores Consistent Porosity Thermal Modified Pore Structure Most importantly, through our proprietary process, we eliate micropores, further improving column efficiency, inertness, and reproducibility. Absence of Micropores.

3 Kinetex LC LC LC C The Chosen Core-Shell Brand Kinetex Core-Shell Technology delivers dramatic improvements in efficiency over conventional fully porous media which can be leveraged to increase resolution, greatly improve productivity, reduce solvent consumption, and decrease costs. Whether you are running HPLC or UHPLC methods, the Kinetex core-shell family can deliver shockingly improved performance over the current column you are using. inely Tuned, Unique Core-Shell Manufacturing Process Phenomenex designs, manufactures, and sells its very own silica and organo-silica coreshell particles. Using silica sol-gel processing techniques that incorporate nano-structuring technology, a durable, homogeneous porous shell is grown on a solid silica core to create a core-shell particle. The combination of a consistent, solid high density core along with proprietary column packing technologies ensures optimum bed structure and high column performance. Solid Core Porous Shell Kinetex Core-Shell ully Porous You can unleash the power of Kinetex Core-Shell Technology columns to achieve faster and better results just not possible on conventional fully porous materials. No matter what type of system you have or type of analysis you are perforg, there is a Kinetex solution for you. 8

4 Gain Incredible Performance With Kinetex and Luna mega The undeniably high efficiency levels found in each Luna mega and Kinetex column provide you with the potential of huge gains in method performance. While traditional silica and hybrid fully porous particles may claim high performance, when compared to Luna mega or Kinetex, they fall short and prevent HPLC/UHPLC scientists from reaching their goals. UHPLC Efficiency Levels (plates/m) Kinetex. Luna mega. Kinetex. Conventional ully Porous. Kinetex.,,,, UHPLC Performance Cyclosporine Impurity Profile Kinetex. μm Luna mega. μm Pressure: bar Pressure: bar Valuable Resolution App ID 9 Valuable Resolution App ID Conventional. μm - Disappointing Resolution Pressure: bar App ID 9 Conditions for all columns same except where noted: Columns: Kinetex. μm Luna mega. μm Conventional ully Porous. μm Dimensions: x. mm Mobile Phase: Acetonitrile/Tert-butyl methyl ether/water/phosphoric acid (:::) low Rate:. ml/ Temperature: 8 C Detection: nm Sample: Cyclosporine - HPLC Efficiency Levels (plates/m) Kinetex. Kinetex. Luna mega Conventional ully Porous Kinetex Luna mega Conventional ully Porous,,,, HPLC Performance Water Soluble Vitas Kinetex. Luna mega aster, er Efficiency Results and Stability Under % ueous Conditions App ID 8 Greater Retention and Stability Under % ueous Conditions App ID 8 8 Core-Shell. Poor Peak Shape and Unable to Run at % ueous Conditions 8 App ID Conditions for all columns same except where noted: Columns: Kinetex. Luna mega Core-Shell. Dimensions: x. mm Mobile Phase: A: mm Potassium Phosphate B: Methanol Gradient: Time () Gradient for conventional core-shell column starts at low Rate:. ml/ Temperature: Ambient Detection: nm Sample:. Thiae. Nicotinamide. Pyridoxal. Pyridoxine. Pantothenic Acid. Riboflavin

5 Gain Retention and Resolution With Enhanced The polar modified functionalities of the and PS stationary phases provide greater polar compound retention and resulting improvement in resolution values. Additionally, the advanced proprietary bonding technology used for both the and PS ensures % aqueous stability as well as balanced retention for non-polar compounds. Nicotine and Metabolites MMA and Succinic Acid.8e.e.e.e.e.8e.e.e.e.e 8.e.e.e.e.e.e.e.8e.e.e.e.e 8.e.e.e.e Greater Retention Conditions for all columns: Columns: Luna mega. Core-Shell. Dimension: x. mm Mobile Phase: A: mm Ammonium ormate with. % ormic Acid B: Acetonitrile with. % ormic Acid Gradient: Time (). 9 low Rate:. ml/ Temperature: C Detection: MS/MS (SCIEX API ) Sample:. Nornicotine. -Hydroxycotinine. Nicotine. Cotinine. Anabasine Columns: Kinetex. Dimension: x. mm Mobile Phase: A: Water B:. % ormic Acid in Methanol Gradient: - in, hold low Rate:. ml/ Temperature: Ambient Detection: MS/MS (SCIEX API ) Sample: Pesticides. ind the full compound list online at Luna mega. μm App ID 9 App ID 9 Core-Shell..e.8e.e.e.e.e 8.e.e.e.e.e.e.e.e Conditions for all columns: Columns: Luna mega. μm PS Conventional ully Porous μm Dimension: x. mm Mobile Phase: A: Water with. % ormic Acid B: Acetonitrile with. % ormic Acid 8.9e 8.e.e.e.e.e.e.e.e Gradient: Time (). Luna mega. μm PS Greater Retention and Resolution Conventional ully Porous μm low Rate:. ml/ Temperature: C Detection: MS/MS (SCIEX API ) Sample:. Succinic acid. MMA ptimal Selectivity for Complex /Non- Mixtures Use the combined core-shell performance and dual polar/non-polar selectivity gains of the Kinetex. to easily expand the elution window and resolution values for your multi-compound methodologies that contain polar and non-polar compounds. App ID App ID 9 App ID Increase Retention with Alkaline Mobile Phase The chemical stability and wide ph range (-) of Kinetex EV organo-silica particles allow you to use high ph mobile phases that are above a basic compound s pk a, thus enhancing retention and potentially improving resolution and sensitivity values. Kinetex. EV 8 ph. Conditions for all columns same except where noted: Column: Kinetex. EV Dimensions: x. mm Part No.: B--AN Mobile Phase: A: mm Potassium phosphate (ph.) B: Methanol Mobile Phase: A: mm Potassium phosphate (ph ) B: Methanol Gradient: Time () 8 low Rate:. ml/ Temperature: º C Detection: nm Sample:. luvoxae 9 Kinetex. EV EV EV Utilize ph Stability of EV to Enhance Retention ph App ID 8 App ID 9

6 Combined Method Development lexibility Expand your method development options by screening a combination of core-shell and fully porous particles with different selectivities which can increase retention, improve resolution, or even flip peak elution orders. The combination of available Luna mega stationary phases represent an outstanding tool set to start with for the separation of acids, bases, neutrals, or mixtures. At the same time, the Kinetex core-shell EV,, and are outstanding orthogonal phases that can easily provide you with highly useful changes in elution windows and retention times. Cold Medicine Ingredients Excellent Resolution Kinetex App ID 8 Good Change in Retention Luna mega 9 8 App ID 8 New Elution Window Kinetex. EV App ID 8 EV 8 Useful Elution rder Movement Kinetex. 8 9 App ID 9 8 Conditions for all columns: Columns: Kinetex. Luna mega Kinetex. EV Kinetex. Dimension: x. mm Mobile Phase: A: mm Potassium Phosphate ph. B: Acetonitrile Gradient: Time () 8 low Rate: ml/ Temperature: Ambient Detection: nm Sample:. Maleic Acid. Phenylephrine. Acetaophen. Doxylae. Pyrilae. Chlorphenirae. Dextromethorphan 8. Bromophenirae 9. -Nitrophenol. Acetylsalicylic Acid. Diphenhydrae Improve Peak Shape for Bases The unique surface of the Luna mega PS contains a positive charged ligand which aids in the retention of acidic compounds through ionic interactions, while also greatly improving the peak shape of basic compounds through ionic repulsion. Conditions for all columns: Column: Luna mega PS Conventional ully Porous Dimensions: x. mm Mobile Phase: A: Water with. % ormic Acid B: Acetonitrile with. % ormic Acid Gradient: - 9 in low Rate:. ml/ Temperature: Ambient Detection: nm Sample:. Amitriptyline. Nortriptyline. Imiprae. Clomiprae Luna mega PS 8 8 Conventional ully Porous 8 8 Better Peak Shape and Sensitivity - App ID App ID

7 Superior Reproducibility And Scalability Batch-to-batch and column-to-column, Luna mega and Kinetex advanced surface chemistries are designed to be consistent and incredibly accurate tools for your analyses. Each batch and column are quality tested to ensure dependability and reproducibility. Additionally, direct selectivity scalability between particles sizes in both product families enables fluid method transfer from UHPLC platforms to HPLC and preparative instrumentation. Batch-to-Batch Reproducibility Steroids Kinetex. Pharmaceutical Mix Luna mega. Batch 8 R s (,). Batch R s (,). Batch R s (,). Batch 8 App ID 8, 8, 8 Batch A App ID Batch Batch B Batch Batch C Seamless Scalability (UHPLC/HPLC/PREP LC) Gingerols Kinetex. μm Kinetex. μm Kinetex. μm Kinetex μm App ID,,, Pharmaceutical Mix Luna mega. μm Luna mega μm App ID Remove harmful contaants and particulates or Longer Column Lifetimes ULTRA UHPLC Column Protection The column protection systems traps contaants and particulates. nce the cartridge is exhausted, simply replace it instead of your column! You will experience: Increased column lifetime er column performance More reproducible chromatography ewer wasted columns Contaants Trapped rit Material Chamber Compounds of Interest Contaants and Particulates * Cartridge schematic not drawn to scale Cartridge Holder rom Injector ULTRA Column to Detector

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9 Luna mega Phase Selection Luna mega Particle Selection Kinetex Core-Shell Particle Selection Complete scalable solution from UHPLC to HPLC to PREP LC UHPLC Rugged and highly efficient with strong focus on hydrophobic retention of non-polar and polar compounds Carbon Load %: Available Particle Size(s):. USP Listing: L PREP LC Excellent UHPLC efficiencies and inertness that drive greater performance gains UHPLC Unique, % aqueous stable mixed-mode phase that provides both polar and non-polar retention. The surface contains a positive charged ligand which aids in the retention of acidic compounds through ionic interactions, while the ligand promotes general reversed phase retention. The positively charged surface also improves basic compound peaks shape through ionic repulsion. Carbon Load %: 9 Available Particle Size(s):.,, USP Listing: L HPLC. PS % aqueous stability and enhanced selectivity/retention for polar analytes without diishing useful non-polar retention. The ligand provides general hydrophobic interactions while a polar modified particle surface provides enhanced polar compound retention. Complete scalable solution from UHPLC to HPLC to PREP LC Kinetex Phase Selection Easily upgrade existing and conventional fully porous methods to gain greater resolution and sensitivity Incredible UHPLC efficiency and performance gains % higher efficiency than fully porous. μm columns Achieve sub- μm performance on HPLC and UHPLC systems EV Combined and polar modified surface that provide polar and non-polar retention alongside % aqueous stability Robust reversed phase methods even in alkaline conditions with improved peak shape for polar basic compounds All purpose phase that offers phase with protective butyl USP L phase that provides the hydrophobic retention side chains for improved peak less hydrophobic and methylene and methylene selectivity shape for basic compounds selectivity than a chromatographers expect from under neutral and acidic a column conditions ph Stability: - Particle Size(s):,.,. USP Listing: L ph Stability:. - 8.* Particle Size(s):,.,.,. USP Listing: L ph Stability:. - 8.* Particle Size(s):,.,.,. USP Listing: L PhenylHexyl ly reproducible pentafluorophenyl propyl phase that offers a unique combination of polar, hydrophobic, aromatic, and shape selectivity % aqueous stable and allows for excellent reversed phase retention and enhanced polar and aromatic selectivity ph Stability: Particle Size(s):,.,. USP Listing: L ph Stability:. - 8.* Particle Size(s):,.,. USP Listing: L PREP LC.... All purpose HPLC and Preparative LC particle capable of improving performance for existing - methods HPLC Instantly improve your pharmacopoeia (Ph. Eur. & USP) monographs that require. μm particle size Carbon Load %: 9 Available Particle Size(s):.,, USP Listing: L ph Stability:. - 8.* Particle Size(s):. USP Listing: L μm or better efficiencies at μm pressures for HPLC and PREP LC methods XB ph Stability:. - 8.* Particle Size(s):,.,. USP Listing: L Reversed phase chemistry Unbonded silica phase for that allows for greater retention conditions to provide selectivity and separation of aromatic for polar compounds hydrocarbons ph Stability:. - 8.* Particle Size(s):,.,. USP Listing: L ph Stability:. -. Particle Size(s):,.,. USP Listing: L *ph stability under gradient conditions. ph stability is.- under isocratic conditions The Ultimate Guide to Reversed Phase HPLC/UHPLC Selectivity Hydrocarbon Compounds Hydroxyl- or Ae-containing unctionalities Kinetex XB- Si column hydrophobicity values indicate greater retention of carbon-containing analytes. USP: L Synergi Hydro-RP Luna () USP: L8 Kinetex Gei USP: L Si USP: L C Luna () Non-ionized Bases and xygenor Halogen-containing Compounds Cation Selectivity USP: L Kinetex USP: L x. G--E G--E G--E G--E G--E G-8-E G--E /pk AJ-99 AJ-9 AJ-9 AJ-88 AJ-88 AJ-8 AJ-8 for. mm ID SemiPrep Cartridges*** x AJ-9 AJ-9 AJ-98 AJ-98 for mm ID x. --P-AX --P-AX --P-AX --P-AX --P-AX -8-P-AX --P-AX --P-AX x. G--P-AX G--P-AX G--P-AX G--P-AX G--P-AX G-8-P-AX G--P-AX G--P-AX x x --U-AX G--U-AX --U-AX G--U-AX --U-AX --U-AX G--U-AX --U-AX G--U-AX -8-U-AX G-8-U-AX --U-AX G--U-AX PREP Cartridges* x. AJ-9 AJ-9 AJ-9 AJ-9 AJ-9 AJ-9 AJ-9 AJ-9 for. mm ID PREP Cartridges** x AJ-9 AJ-9 AJ-9 AJ-9 AJ-9 AJ-9 AJ-9 for mm ID. μm MidBore Columns (mm) x. EV XB- A-9-Y A--Y A-9-Y A--Y. μm Analytical Columns (mm) x. EV XB- A--E. μm Minibore Columns (mm) x. EV XB- A-98-AN A--AN A-99-AN A--AN. μm MidBore Columns (mm) x. XB- A-98-Y A-99-Y x. B--AN B-9-AN B--AN B--AN B-9-AN B--AN B-9-AN B--AN B-9-AN x. B--Y B-9-Y B--Y B--Y B-9-Y B--Y B-9-Y B-9-Y x. B--E B-9-E B--E B--E B-9-E B--E B-9-E B--E B-9-E x. B--AN B--AN B-8-AN B-98-AN B--AN B-99-AN B--AN B--AN x. B-98-Y B--Y B-99-Y B--Y x. B--AN B--AN x. D--Y D--Y x. D--E D--E x. D--AN D--AN x. --Y --Y x. --E --E μm Axia Packed Preparative Columns (mm) x. x. x --P-AX G--P-AX --U-AX PS --P-AX G--P-AX --U-AX Catiovn Selectivity at ph. x. --AN -9-AN --AN --AN -9-AN --AN -9-AN --AN -9-AN x. --Y -9-Y --Y --Y -9-Y --Y -9-Y --Y -9-Y /pk AJ-99 AJ-9 AJ-9 AJ-98 AJ-8 AJ-8 AJ-8 AJ-89 AJ-88 for. mm ID x. D--E D-9-E D--E D--E D-9-E D--E D-9-E D--E D-9-E x. --E -9-E --E --E -9-E --E -9-E --E -9-E /pk AJ-99 AJ-9 AJ-9 AJ-9 AJ-88 AJ-88 AJ-8 AJ-8 AJ-8 for. mm ID x. D--AN D--AN D-8-AN D-98-AN D--AN D-99-AN D--AN D--AN x. --AN --AN -8-AN -98-AN --AN -99-AN --AN /pk AJ-998 AJ-9 AJ-99 AJ-88 AJ-88 AJ-88 AJ-88 AJ-888 for. mm ID x. D-98-Y D--Y D-99-Y /pk AJ-8 AJ-8 AJ-8 AJ-89 for. mm ID x. C-9-AN C--AN C-9-AN C--AN C-9-AN x. C-9-Y C--Y C-9-Y x. C-9-E C--E C-9-E C--E C-9-E x. D--AN D-9-AN D--AN D--AN D-9-AN D--AN D-9-AN D--AN D-9-AN x. D--Y D-9-Y D--Y D--Y D-9-Y D--Y D-9-Y D-9-Y. μm Minibore Columns (mm) x. x. A--AN B--AN. μm Minibore Columns (mm) x. A-8-AN PS A--AN A--AN x. G--E G--E x.* AJ- AJ-.-8. mm μm Minibore Columns (mm) x. A--AN PS A-8-AN x.** AJ- AJ-8. mm x. AJ- AJ-9 mm μm MidBore Columns (mm) x. B--Y PS B-8-Y x. AJ- AJ-9 mm μm Analytical Columns (mm) x. B--E PS B-8-E x. B-8-AN B--AN B--AN x. B--AN B-8-AN x. D--Y D-8-Y x. D--E D-8-E x. -8-A --A x. D-8-AN D--AN D--AN x. D--AN D-8-AN x. --Y -8-Y x. --E -8-E x. -8-AN --AN --AN x. --AN -8-AN /pk AJ-9 AJ-98 AJ-9 for. mm ID x.* AJ- AJ-. -. mm x.* AJ- AJ-. -. mm x. G--E G-8-E x.* AJ- AJ-.-8. mm Luna () Which solid support is right for your analysis? If Kinetex or Luna mega analytical columns do not provide at least an equivalent separation as compared to a competing column of the sameparticle size, similar phase and dimensions, return the column with comparative data within days for a ULL REUND. Important! x.** AJ- AJ-8. mm x.* AJ- AJ-. -. mm. μm Microbore Columns (mm) x. x. B-8-A D-8-A B--A D--A ULTRA Cartridges require holder, Part No.: AJ-9 * Analytical Cartridges require holder, Part No.: KJ-8 ** PREP Cartridges require holder, Part No.: AJ-8 PREP Cartridges require holder, Part No.: AJ-8 nly column selectivity parameters of the same class (i.e. Steric Interactions) may be compared between the columns featured here. μm Axia Packed Preparative Columns (mm) x x G--U-AX G--V-AX PS G--U-AX G--V-AX x. --AN --AN x.* AJ- AJ-. -. mm Hydrophobic selectivity is the main mechanism of retention under reversed phase conditions. Within the column profiles the different selectivity parameter classes are not on the same scale. Performance Gains on ANY HPLC or UHPLC System Extensive Selection for Increased Retention Core-Shell Particles ully Porous Particles Increased loadability due to higher surface area Excellent mechanical strength Large range of scalable particle sizes and selectivities Ultra-high efficiency at decreased backpressure (. ) or better efficiencies at pressures ( ) Easy method transfer between HPLC and UHPLC systems est efficiencies on UHPLC systems (. and. ) Additional Selection Tips Acids Luna mega PS Alkaline Mobile (ph 8-) Aromatic Compounds Synthetic ligonucleotides Proteins (> kda) Peptides ( kda) Chiral Compounds Kinetex Kinetex Luna PP() Clarity ligo-xt Clarity ligo-rp Aeris WIDEPRE XB- Aeris WIDEPRE XB- Aeris WIDEPRE C Aeris PEPTIDE XB- Luna mega PS Kinetex EV Kinetex EV Gei NX- Gei Gei C-Phenyl x. -9-A /pk AJ-998 AJ-9 AJ-9 AJ-99 AJ-88 AJ-88 AJ-88 AJ-88 AJ-888 for. mm ID Kinetex EV Luna mega Synergi Max-RP Improved Peak Shape for Bases Luna mega PS USP: L8 t Gei Luna PP() Increased Retention of Bases Kinetex x. --E --E --E --E --E -8-E --E USP: L column cation selectivity values will have less interaction and retention for ionized bases, but may have very good peak shape. μm Axia Packed Preparative Columns (mm) x x EV B--U-AX D--U-AX B--U-AX D--U-AX XB- B--U-AX D--U-AX B--U-AX D--U-AX B-8-U-AX D-8-U-AX B--U-AX D--U-AX μm Analytical Columns (mm) x. B--E PS B--E column cation selectivity values will show higher retention for ionized bases. μm Axia Packed Preparative Columns (mm) x. x. EV B--P-AX D--P-AX B--P-AX D--P-AX XB- B--P-AX D--P-AX B--P-AX D--P-AX B-8-P-AX D-8-P-AX B--P-AX D--P-AX D--P-AX μm MidBore Columns (mm) x. B--Y PS B--Y Synergi Hydro-RP Isomers - /Neutral unctionalities Positional Kinetex x. D--E D--E D--E D--E D--E D-8-E D--E μm Semi-Preparative Columns (mm) x x EV --N G--N G--N --N G--N --N G--N μm Minibore Columns (mm) x. A--AN PS A--AN Hydrogen bond donating groups on the silica surface interact with accessible functionalities containing a lone pair of electrons. column steric interaction values are best suited for the analysis of analytes that require separation based on size and shape differences. USP: L8 s x. --Y --Y --Y --Y --Y /pk AJ-99 AJ-9 AJ-98 AJ-8 AJ-8 AJ-8 AJ-88 for. mm ID. μm Minibore Columns (mm) x. EV A--AN A-9-AN A--AN A--AN XB- A-9-AN A--AN A-9-AN A--AN A-9-AN MEGA B x. --AN --AN --AN /pk AJ-998 AJ-9 AJ-99 AJ-88 AJ-88 AJ-88 AJ-888 for. mm ID. μm Microbore Columns (mm) x. x. XB- B-9-A D-9-A H Synergi Max-RP Kinetex μm Analytical Columns (mm) x. EV B--E B--E B--E XB- B--E B--E B-8-E B--E B USP: L Kinetex Basic Compounds Kinetex EV Identify Differences in Shape Selectivity Luna () Luna Luna mega PS x. D--Y D--Y D--Y D--Y D--Y D-8-Y D--Y x. D--AN D--AN D--AN D--AN D--AN D-8-AN D--AN ULTRA Cartridges require holder, Part No.: AJ-9 * PREP Cartridges require holder, Part No.: AJ-8 ** PREP Cartridges require holder, Part No.: AJ-8 *** SemiPrep Cartridges require holder, Part No.: AJ-98 Luna mega PS Luna CN N Gei NX- Isomers, Isobaric Compounds, and Shape Selectivity Luna mega Luna () Kinetex Hydrogen bond accepting groups on the silica surface interact with hydrogen bond donating functionalities on analytes. Synergi usion-rp Kinetex Synergi usion-rp x. B--AN B--AN B--AN B--AN B--AN B-8-AN B--AN Kinetex Luna mega Kinetex XB- Synergi Max-RP Kinetex USP: L Synergi -RP X μm MidBore Columns (mm) x. EV B--Y B--Y B--Y XB- B--Y B--Y B-8-Y B--Y Gei H μm Minibore Columns (mm) x. EV A--AN A--AN A--AN XB- A--AN A--AN Luna PP() Gei C-Phenyl. μm Analytical Columns (mm) x. x. /pk XB- D--E --E AJ-88 for. mm ID Core-Shell Technology Luna mega Lux Amylose- Lux Cellulose- Lux Cellulose- Lux i-cellulose-