ect of Protamine from Chum Salmon Milt on Lipid Absorption in Humans

Transcription

1 360 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. //, No. 2, -0* -00 (,**2) 6 * * The Suppressive E# ect of Protamine from Chum Salmon Milt on Lipid Absorption in Humans Yosuke Hoshino, Yoshinori Takahashi, Masataka Kawarasaki, Ryoko Akita, Hiroyuki Enari and Sigeru Yamamoto* Central Research Laboratory, Nichiro Corporation, +0, Wadai, Tsukuba-City, Ibaraki -**.,3/, Japan * International Nutrition, Ochanomizu University Graduate School of Humanities and Sciences,, + + Ohtsuka, Bunkyo-ku, Tokyo ++, 20+*, Japan Protamine is a basic protein that is combined with DNA in the vertebrate sperm nucleus. It was reported that protamine purified from chum salmon milt has pancreas lipase inhibition activity (IC/* +,. g/ml). In the case of rats, protamine administration ( +** mg/rat) showed a suppressive e# ect on the blood plasma triglyceride (TG) levels in the corn oil tolerance test. Here, we investigated the suppressive e# ect of our developed protamine, Proserve, on lipid absorption in humans. The oral fat tolerance test (OFTT) was performed on normal adult male subjects as a single-blind test. The subjects were fed a high-fat meal containing /0.* g of lipid and /** mg of protamine. Before and,, -,., 0 h after ingestion, TG and free fatty acid levels in blood were measured. Protamine significantly suppressed the elevations of the TG and free fatty acid levels in blood after ingestion of the high fat meal compared with the placebo group. When -** mg of protamine was administered, no significant suppressive e# ect was confirmed, but TG and free fatty acid levels in the blood tended to be suppressed. In the digestive resistance test, the lipase inhibition activity of protamine was maintained after the reaction in the stomach. Therefore, we propose that protamine is an anti-obesity material. (Received Mar. -+,,**2 ; Accepted May,-,,**2) Keywords : salmon, milt, protamine, lipid absorption :, in vitro - TG TG IC /*. g ml, DNA DNA TG +,-,+/ **--, **.,3/ +0, * ++, 20+*, + + Corresponding authoryo-hoshino@maruha-nichiro.co.jp +, + -, --. *** / ***

2 7 : 361 AI : PRRRRSSSRP VRRRRRPRVS RRRRRRGGRR RR + +** g AII : PRRRRSSSRP IRRRRPRRAS RRRRRRGGRR RR CII : MPRRRRSSSR PVRRRRRPRV SRRRRRRGGR RRR + ( ) kcal g -00 /4, -1-, AI, AII, CII g / * g g,- // 3*.. - in vitro +** mg TG - C +2 :, -4, C +2 : - *4- C,* : * *40,43 +.-Methylumbelliferyl oleate. -MUO BioChemika EC EC -.,-+... EC -.,+.... SIGMA +,,*.* g 240 /4/ *4+ mg,-2, 0*,/, OFFT Cream wv + +0* g -/ /04* +4/*,4. *4/* *42 *4-0 *40 *4,/ *4. *4*, *4* 0, , kcal/ +** g /.1 kcal - OFFT Cream C. : *.4, C 0 : *,4, C 2 : * +4, C +* : *,41 C +* : + *4- C +, : * -4* C +. : * +*4, C +. : C +0 : *,34- C +0 : +,4- C +2 : * +*43 C +2 : +, InBody -,.. Oral Fat Tolerance Test : BMI, OFTT,+,+ - +,/* -** mg,/* mg PCS FC- /* : OFFT Cream /0.* g

3 362 // 2,**2 2 8, - +** ml +.* gml /* ** mg -** mg + /** mg IC +., gml,/* mg, + /** mg,/* mg 0, OFFT Inbody, -. 0 /. :TG FFA T-Cho LDL LDL-Cho HDL HDL-Cho / / /0 / 1 - TG OFFT, - MacIlvain +** mmoll TG -,** mmoll. /** mg ph 1...-Methylumbelliferyl, a, - oleate. -MUO 1+.. p**/. -** mg moll.-muo +.* L, /* moll,* gml - a 0 +* L, +.* gml TG AUC Area Under the Curve -1 / /** mg., /* L, /* mu -1,*, b p*.*/ -** mg +1 +** mmoll +.* ml - b TG 30 Microfluor + Black, Thermo Labsystems 1* L +** mmoll. +.* L Tecan GENIOS Pro -,* nm, OFFT. /./* nm /** mg 0. a - p*. + -** mg / a 0 AUC /** mg,** mg +* mmoll./. b p*.*/ -** ph 0. 2,* ml -1, mg,- / b +*. moll +*. mol TG, L +*. ml ph +/. +*. mgml +*. ml -1 TG 0* +.* moll,*. ml +* 1 23,+ +, Student to / +* + in vitro TG TG /*

4 9 : 363 TG. Inbody n1 n0 p / -* +,3 + *401 LDL cm +1, / *41 *412 HDL kg 1* * -4- *4/* 0 kg /-4+,41 /,40,4* *423-0 kg ,4. +4/ *4-.,* / *4, *42 2, *4,, BMI, ,,40 +4* *4/+ 0 in vitro BMI Body Mass Index p Student s t, -** mg n1 n0 a b AUC Area Under the Curve - /** mg n1 n0 a b AUC Student s t * p**/..

5 364 // 2,** ** mg n1 n0 a b AUC / /** mg n1 n0 a b AUC Student s t * : p**/. : p*+. 1 OFFT in vitro,** /** m mol/l +* +, +** mmol/l 0* +.* mmol/l +- 1 DNA DNA

6 11 : a n1 -** mg n0 b n1 /** mg n0 1 +*. gml +*. gml n/ OFTT /** mg TG

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