PHARMACODYNAMIC STUDY OF MONGOLIAN MEDICINE JIRUHEN NEW NO.2 ON THE MYOCARDIAL ISCHEMIA IN RATS

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1 458 -Ⅱ Ⅱ 6 -Ⅱ BL-420 S NO LDH CK T ST NO LDH CK P< Ⅱ P<0.05 T ST NO LDH CK P<0.01 -Ⅱ -Ⅱ R969.4 A DOI: /j.cnki.issn x X PHARMACODYNAMIC STUDY OF MONGOLIAN MEDICINE JIRUHEN NEW NO.2 ON THE MYOCARDIAL ISCHEMIA IN RATS WU Yu-xiao LI Li SA Ren Gaowa et al. Tianjin University of Traditional Chinese Medicine Tianjin China Abstract Objective To explore the rapeutic effect of Mongolian medicine Jiruhen new No.2 new No. 2 on myocardial ischemia. Methods Using comprehensive methods such as feeding fatigue establish " qixu" model of myocardial ischemia model in rats.rats were randomly divided into normal group model group control group new No.2 high medium low dosage group each group was administered by corresponding drug and examined ECG and serum NO LDH CK.Results After modeling compared with the normal group there are significant ischemic change in ECG and serum NO LDH CK P < After treatment with new No. 2 compared with the model group No. 2 significantly improve the ischemic change in ECG and serum NO LDH CK P < 0.05 or P < Conclusion NJZZ MS changbai2010@ aliyun.com

2 Middle dose of Mongolian Medicine Jiruhenxin-new No.2 has better effects on myocardial ischemia. Key words mongolian medicine jiruhenxin-new No.2 myocardial ischemia model pharmacodynamie Ⅱ BL-420S 9~ 30s 1mim 2min 12 coronary heart diease CHD 3000r /min 15min 3~ 6 13~ CK LDH NO 15 -Ⅱ 1.3 SPSS 17.0 x±s 珋 P<0.05 -Ⅱ wk SPF Wistar SCXZ ± 20 g -Ⅱ wk T ST NO LDH CK P < wk -Ⅱ 60mg /ml d 10min 5wk 0.2g /kg 2.1 P < Ⅱ -Ⅱ 1g /kg - P< Ⅱ 0.5g /kg -ⅡⅡ 0.25g /kg 6wk 2 /d 1 ~ 6 1 ~ 6 1 Tab.1 -Ⅱ / n = 10 x±s 珋 Effects of Jiruhen-new No.2 on heart rate times /min of ECG in rats n = 10 珋 x±s 485.0± ± ± ± ± ± ± ± ±12.9 -Ⅱ 460.2± ± ±10.6 -Ⅱ 490.2± ± ±8.6 -Ⅱ 456.0± ± ±9.9 P<0.01 P<0.01

3 Ⅱ T mv n = 10 x±s 珋 Tab.1 Effects of Jiruhen-new No.2 on T wave mv of ECG in rats n = 10 珋 x±s 0.07± ± ± ± ± ± ± ± ± Ⅱ 0.06± ± ± Ⅱ 0.08± ± ± Ⅱ 0.08± ± ±0.067 P<0.01 P< Tab.3 -Ⅱ ST mv n = 10 x±s 珋 Effects of Jiruhen-new No.2 on ST segment mv of ECG in rats n = 10 珋 x±s 0.178± ± ± ± ± ± ± ± ± Ⅱ 0.196± ± ± Ⅱ 0.181± ± ± Ⅱ 0.187± ± ±0.017 P<0.01 P< Tab.4 -Ⅱ NO μmol /L n = 10 x±s 珋 Effects of Jiruhen-new No.2 on serum NO concentrations μmol /L in rats n = 10 x±s 珋 70.58± ± ± ± ± ± Ⅱ 42.95± ± Ⅱ 42.94± ± Ⅱ 41.77± ±9.11 P<0.01 P< Tab.5 -Ⅱ LDH U /L n = 10 x±s 珋 Effects of Jiruhen-new No.2 on serum LDH concentrations U /L in rats n = 10 x±s 珋 557.5± ± ± ± ± ± Ⅱ ± ± Ⅱ ± ± Ⅱ ± ±114.8 P<0.01 P< Tab.6 -Ⅱ CK U /L n = 10 x±s 珋 Effects of Jiruhen-new No.2 on serum CK concentrations U /L in rats n = 10 x±s 珋 997.1± ± ± ± ± ±96.9 -Ⅱ ± ±84.9 -Ⅱ ± ± Ⅱ ± ±137.5 P<0.01 P<0.01

4 Fig.1 ECG of rats in normal group 2 Fig.2 ECG of rats in model group 3 Fig.3 ECG of rats in control group Fig.4 4 -Ⅱ ECG of rats in Jiruhen-new No.2 high dose group Fig Ⅱ 6 -Ⅱ ECG of rats in Jiruhen-new No.2 medium dose group Fig.6 ECG of rats in Jiruhen-new No.2 low dose group 18 -Ⅱ LDH CK NO T ST 19~ 22 -Ⅱ -Ⅱ -Ⅱ M M. 16 CHD

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