Chinese Journal of Experimental Traditional Medical Formulae 14 ~ ~ 20 P < 0. 05
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1 * SHR 14 ~ SHR 55 SHR WKY ELISA HT Ⅱ ANGⅡ NE 14 ~ 20 P < HT ANGⅡ NE 4 6 P < ~ 18 5-HT ANGⅡ NE 14 ~ 18 SHR 5-HT ANGⅡ NE R doi /j. cnki. syfjx A Research of Relative indexes of Animal Models of Hypertension with Liver Fire Hyperactivity Syndrome XUE Xiao-xing 1 LI Yu-bo 2 LIAN Hong-jian 1 LI Jun-ling 1 YAN Jing-lu 1 MA Xue-ling 1 WANG Wei-ming 1 WANG Wei 1* 1. School of Preclinical Medicine of Beijing University of Chinese Medicine Beijing China 2. Institute of Basic Theory of Traditional Chinese Medicine of Chinese Academy of Chinese Medical Sciences Beijing China Abstract Objective To investigate the biological basis of hypertension with hyperactivity of liver fire of macro characterization and behavioral tests micro physicochemical indexes disproof prescriptions. Method Fifty-five SHR rats with hyperactivity of liver fire were divided into model group Sancao Jiangya decoction SCD group Sini San decoction SND group and nifedipine group according to Latin square method Wistar-Kyoto WKY rats as control group. The macro characterization behavioral indexes and the systolic blood pressure SBP were collected. Enzyme-linked immunosorbent assay ELISA was used to detect the serum concentration of 5-hydroxytryptamine 5-HT angiotensin Ⅱ ANG Ⅱ norepinephrine NE after the rats were administrated for 4 and 6 weeks. Result Compared with control group the SBP and irritable degree increased revolving tolerance time decreased and serum 5-HT ANGⅡ NE level was higher in model group. Compared with model the SBP in SCD group and nifedipine group reduced after treated for 4 6 weeks weeks of age the SBP in SND group was stable and increased in the 16 weeks and then decreased. The symptoms of liver fire hyperactivity improved and it was not obvious in SND group and serum 5-HT ANG Ⅱ NE level was lower in SCD group compared with model group. Conclusion Fourteen-eighteen weeks is the stable time window of hyperactivity of liver fire. SHR 5-HT ANGⅡ NE may be used as the objective index of hypertension of hyperactivity of liver fire syndrome CB JYBZZ-XS-026 Tel xuexiaoxing2012@ 126. com * Tel wangwei@ bucm. edu. cn 97
2 21 8 Key words spontaneously hypertensive rats hyperactivity of liver fire biological mechanism kg ig 10 ml kg SHR 14 ~ 18 g kg g kg g kg ig 2. 3 SBP d 5-5-HT Ⅱ ANGⅡ 2. 4 NE HT Ⅱ ANGⅡ NE SHR 55 WKY 12 SPF 60 r min - 1 SCXK min SoftronBP-98A Ⅲ JD-01GSZ 3 Ⅱ 2 Ⅰ 1 5-HT ANGⅡ NE ELISA 0 0 RD EIA EIA g 20 g 20 g 20 g 6 g 6 g 6 g 6 g 6 g HT ANGⅡ NE % 4 ml kg ml H ~ 2 h r min min ELISA 2 5-HT ANGⅡ NE 2. 1 SHR WKY SPSS x 珋 ± s 1 6 ANOVA t P < SHR WKY ig 1 /d 6 P <
3 P < Table 1 x 珋 ± s Comparison of SBP in rats of all groups in different administration time 珋 x ± s n = 12 /mmhg 2 n = 12 4 n = 12 6 n = ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± P < P < mmhg = kpa SHR % 33% % % 2 P < % % % P < Table 2 x 珋 ± s Comparison of irritable degree in rats of all groups in different administration time 珋 x ± s n = 12 / 2 n = 12 4 n = 12 6 n = ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± P < P < 0. P < NE 6 20 P < P < HT P < ANGⅡ18 ANGⅡ P < P < NE 5-HT P < HT
4 Table 3 x 珋 ± s Comparison of revolving tolerance time in rats of all groups in different administration time 珋 x ± s n = 12 /s 2 n = 12 4 n = 12 6 n = 6-25 ± 6 28 ± 7 22 ± 5 24 ± ± 7 25 ± 4 34 ± ± ± ± 8 25 ± 2 30 ± ± ± ± 6 29 ± ± ± ± ± Table P < P < r min min 120 s 5-HT ANGⅡ NE x 珋 ± s n = 6 Comparison of concentration of serum 5-HT ANGⅡ NE in rats of all groups in different administration time x 珋 ± s n = HT /μg L ANGⅡ /ng L - 1 NE /μg L ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± P < P < HT ANGⅡ NE 5-HT ANGⅡ NE SHR 14 ~ 18 SHR 14 ~ 18 SHR14 ~ HT HT2A 10 K + 2. J. 11 ANG Ⅱ NE HT 5-HT J HT J RAS ANG Ⅱ RAS ANG Ⅱ 5. ANGⅡ H 2 O 2 J NE ANGⅡ NE J. J
5 Dewachter L Adnot S Fadel E et al. Angio poietin / J. tie2 pathway influences smooth muscle hyperplasia in Care Med J G- 9. J. J Gamoh S Hisa H Yamamoto R. 5-hydroxytryptamine receptors as targets for drug therapies of vascular-related diseases J. Biol Pharm Bull Sung D J Noh H J Kim J G et al. Serotonin contracts the rat mesenteric artery by inhibiting 4-aminopyridinesensitive Kv channels via the 5-HT2A receptor and Src tyrosine kinase J. Exp Mol Med e67. doi /emm Singh P Fletcher T W Li Y et al. Serotonin uptake rates in Platelets from angiotensin II-induced hypertensive mice J. Health Irvine Calif A idiopathic pulmonary hypertension J. Am J Respir Crit 5- J Du C Q Yang L Yang J et al Inhibition of farnesyl pyrophosphate synthase prevents norepinephrine-induced fibrotic responses in vascular smooth muscle cells from spontaneously hypertensive rats J. Hypertens Res J 檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶檶 Ⅰ I I When different dosages of improved accessory I combine with dextrin viscosity and release are positively correlated When different dosages of improved accessory I combine with dextrin viscosity and release are negatively correlated 1 101
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