Experimental Techniques Training Series
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1 Pressure Volume Assessment: Rodent Cardiac Function Training Course 2-Day Training Course for the Cardiovascular Scientist Thursday, August 2nd Friday, August 3rd, :00 pm - 5:00 pm 8:30 am - 5:00 pm SpringHill Suites by Marriott Plato BioPharma, Inc West 103rd Ave W. 103rd Ave. Suite 300 Westminster, CO Westminster, CO This training course will focus on surgical skills, catheter placement, calibration and data recording methodologies for optimum in vivo left ventricle Pressure-Volume measurements. Pressure-Volume loops and cardiac function will be analyzed. Don t miss this opportunity to observe live animal surgery demos on the widely accepted rat heart model. Continuous beat-by-beat assessment of left ventricular pressure & volume from one catheter in intact hearts makes this the preferred method for investigations involving phenotyping, gene manipulation, and pharmacological studies. Conductance measurements for estimating LV volume are not only precise and less invasive, but are also a cheaper alternative to traditional systems such as echocardiography, sonomicrometry, and thermodilution. A panel of experts will be on hand to provide the crucial information needed to successfully install and maintain a pressure volume system. Avoid common pitfalls and take the right steps to streamline your physiological data acquisition and produce valid results from day one. Maximize your data investment with powerful analysis toolsets. The A-Z of pressure volume in a two-day intensive learning format. There is no other seminar or workshop like this anywhere! Register now, there are only 10 spaces available. Reasons You Should Attend: You are planning to implement your very own pressure volume system from the ground up. You prefer to make smart decisions on how to adapt pre-existing equipment to accommodate a pressure volume system. You recently purchased or inherited pressure volume equipment and are in need of a refresher course for you and/or your staff.
2 Training Course Program Level I - Ventricular Pressure-Volume Basics This session will introduce attendees to the background physiology, equipment functionality, and experimental design considerations required to fully optimize Ventricular Pressure-Volume systems in a laboratory setting. Learning Objectives The purpose of this session is to familiarize attendees with: Comparative Cardiac Physiology A review of basic cardiovascular physiology Pressure-Volume Loops Pressure-Volume Loops are introduced, focusing on important landmarks of the cardiac cycle Disease States Systolic Dysfunction, Diastolic Dysfunction and Valve Diseases will be discussed along with the corresponding changes in PV Loop morphology Heart Mechanics Important Pressure-Volume parameters are introduced with their application to cardiac function Pressure-Volume System Technology and Theory Introduction to conductance catheter theory and MPVS system operation Calibration and Data Analysis Requirements for proper cuvette calibration of PV data is discussed, along with parallel conductance calibration theory Experimental Design Proper experimental protocols and procedures are introduced Level II - Ventricular PV Experimental Techniques This session will provide comprehensive training for Pressure-Volume experimental techniques. Attendees will receive expert demonstration, as well as and hands-on experience operating Pressure-Volume hardware and performing surgical procedures. Learning Objectives The purpose of this session is to familiarize attendees with: Comparative Cardiac Physiology A review of basic cardiovascular physiology Pressure-Volume Loops Pressure-Volume Loops are introduced, focusing on important landmarks of the cardiac cycle Disease States Systolic Dysfunction, Diastolic Dysfunction and Valve Diseases will be discussed along with the corresponding changes in PV Loop morphology with parallel conductance calibration theory Experimental Design Proper experimental protocols and procedures are introduced
3 Blood Volume Cuvette Calibration Technique for catheter calibration and deriving blood resistivity Saline Calibration Proper technique for assessment of parallel conductance of the myocardium Real-Time Assessment Using LabChart s PV Loop module and other features to acquire relevant data in real time Level III - Leveraging Pressure-Volume Data The final session will take attendees from the acquisition of Pressure-Volume data to producing quality data, ready for publication using the PV Loop module in LabChart 7. Learning Objectives The purpose of this session is to familiarize attendees with: Applying Calibration Techniques Utilizing recorded blood cuvette data to calibrate conductance to volume units Saline Bolus Data Analysis Using the PV Loop module to adjust recorded volume data and derive parallel conductance of the myocardium PV Catheter Maintenance Best practices for prolonging the life of your MPVS systems and Pressure-Volume catheters PV Loop Data Analysis Leveraging recorded data using the PV Loop module and producing quality results Defining & Reporting Key Hemodynamic Parameters Exporting images and relevant cardiovascular hemodynamics for publication Advanced LabChart Techniques Harnessing the power of LabChart 7 to make recording and analyzing data as simple as possible
4 Training Course Instructors: Dr. Craig Plato CEO & Founder, Plato BioPharma, Inc. Dr. Plato has over 10 years experience in the pharmaceutical industry and served as Head of In Vivo Pharmacology at both Myogen and Gilead Colorado from He established Plato BioPharma, Inc. in 2010 and has served small and large Biotech and Pharma in drug discovery. While directing the cardiovascular group at Myogen, Craig s team led several drug discovery pharmacology projects including Histone Deacetylase Inhibitors, Protein Kinase D Inhibitors, and a-myosin Hevay Chain activators for the Myogen-Novartis collaboration focusing on heart failure and pathological cardiac hypertrophy. Dr. Plato provided the first direct evidence and patented the use of HDAC inhibitors as combination therapeutics for the treatment of cardiac dysfunction. At Gilead Sciences, Dr. Plato led CKD drug discovery efforts as well as participated in development activities of the Endothelin Receptor Antagonist program and managed the team responsible for generating preclinical data requested by the FDA for approval of Ambrisentan Orphan Drug status for Idiopathic Pulmonary Fibrosis in Prior to these efforts, Craig was a Senior Scientist responsible for leading cardiorenal drug discovery teams at Abbott Laboratories ( ) in the Chicago area where he focused on diabetic nephrology, electrophysiologic safety, and platelet and coagulation activation. Dr. Plato received his Ph.D. Degree in Physiology from the Medical College of Wisconsin (1996), and completed his Post-Doctoral training in Hypertension and Vascular Research at Henry Ford Hospital. During Craig s academic career, he was twice awarded the American Physiological Society (APS) Excellence in Renal Research Award and the American Heart Association Merck New Investigator Award. During Craig s industry career he has served on the APS Renal Section Steering Committee ( ) as well as sat on, then Chaired, the APS Physiologists in Industry Committee. Jeff Harris Global Training & Support Manager, ADInstruments As a member of ADInstruments Corporate Training & Support Team, Jeff has extensive experience providing hands-on training and technical support to a large customer base of cardiovascular researchers focused on in vivo pressure-volume measurements in a wide range of animal models to include mice, rats, rabbits, dogs, pigs, sheep, and horses. Jeff s strong background in biomedical engineering and heart mechanics proved invaluable during the recent design and development of ADInstruments new PV Loop Analysis Module. Jeff is constantly designing, revising, and implementing newly structured training programs to help researchers master successful Pressure Volume hardware/software integration in their laboratory. Jeff received his B.S. Degree in Biomedical Engineering, with minors in Mathematics and Pre-Med, from Saint Louis University (2003.)
5 Brandon Bucher - Research Product and Support Manager, ADInstruments As Research Product Manager at ADInstruments, North America, Brandon has developed and delivered hands-on training and technical support to a wide range of cardiovascular physiologists, including hardware and application implementation and LabChart Software Training. Brandon s background in biomedical engineering and previous work designing specialized laboratory instrumentation delivers an exceptional understanding of the underlying theory, mechanics and operation of Ventricular Pressure-Volume Systems, with a strong grasp of the necessary data collection and analysis tools that are key to the experiment. Brandon received his B.S. in Bioengineering from Washington State University (2006). Kristin Joly Plato BioPharma, Inc. Kristin Joly has cardiovascular drug discovery experience at Myogen, Gilead Sciences and since its inception, at PBI. Kristin has been performing direct systemic and cardiac hemodynamic measurements since As part of that experience, Kristin contributed extensively to systolic and diastolic heart failure model development, validation and compound efficacy testing for the Myogen-Novartis heart Failure collaboration. Later, Kristin s expertise was expanded to developing and applying the Millar catheter and ADInstruments PowerLab technology to direct evaluation of pulmonary hemodynamics in the setting of Pulmonary Arterial Hypertension and Idiopathic Pulmonary Fibrosis. Kristin has contributed to several published efforts utilizing the Millar-ADInstruments platform to evaluate cardiac performance, and recently to a peer-reviewed paper (Liles, JT, KK Ida, KM Joly, J Chapo and CF Plato. Age exacerbates chronic catecholamine-induced impairments in contractile reserve in the rat. Am J Physiol Regul Integr Comp Physiol August : R491-R499) evaluating cardiac contractile reserve in the setting of disease with mechanistically distinct inotropic agents. Since 2006, Kristin has performed over 2,000 P-V procedures as well as well over 1000 pulmonary arterial catheterizations. This expertise has been leveraged to both train PBI staff as well as serving as a technical expert at the ADInstruments-Millar Rodent PV workshops since 2010.
6 Training Sponsors: Plato BioPharma, Inc. (PBI) is a pre-clinical drug discovery contract research organization (CRO.) Their decades of pharmaceutical and biotech industry experience result in the focused, data intensive, communicative approach that helps clients navigate the complex drug discovery process more rapidly and effectively. With laboratories designed and opened in 2008, the company is a leader in models of Cardiovascular, Renal, Pulmonary, and Hepatic diseases. PBI utilizes state-ofthe-art data acquisition platforms, and offers a comprehensive suite of research services ranging from pharmacokinetics and target-based functional screens through complex proof-of-concept studies. PBI is based in Denver, CO. Established in 1988, ADInstruments is a world-leading provider of computerbased data acquisition and analysis systems for Life Science. Their products enable users to record and analyze life science data quickly and efficiently, whether it is to further their academic or private industry research, or to advance understanding of scientific concepts in biomedical classrooms. ADInstruments product range is based on the world-renowned PowerLab data acquisition system with LabChart software. PowerLab holds a leadership position in its market sector and is used in universities, hospitals, research institutes, pharmaceutical companies, and contract research organizations. ADInstruments and Plato BioPharma have established a strategic partnership agreement to improve in vivo pre-clinical research practices by providing advanced education courses with hands-on world-class instruction in a state-of-the-art facility, featuring the latest advances in life science research equipment technologies. Millar Instruments, Inc. designs, manufactures, and markets pressure-sensing technology for use by medical researchers and clinicians and has directed this expertise toward manufacturing a complete line of Mikro-Tip pressure and pressure-volume catheters for life science applications requiring complete hemodynamic function assessment. Millar improve research development in the medical community. They will be the exclusive supplier of high-fidelity catheters and pressure-volume systems to the Plato BioPharma training courses. By developing products and systems that further promote medical advancements and discoveries, Millar is committed to being an integral part of this on-going research.
7 ADInstruments in Research for Scientists Title: First name: Last name: Name of additional attendees: Company/Institution: Department/Division: Address for correspondence: City: State: Zip: Country: Phone number: address: Where did you hear about the workshop? Website Sales Rep Colleague Other I will be attending the Pressure Volume Training Course beginning (mm/dd/yyyy) / / at Plato BioPharma, Inc. The registration fee is $995 for industry and $895 for academia. *Please initial Please initial if you have any special needs or dietary restrictions we can plan for to make your day of participation more enjoyable. Details: Payment & Cancellation Training registration is not official until you have received an ADInstruments invoice confirming your registration. Please do not make travel plans until you have received an enrollment confirmation from ADInstruments. Deadline for registration is 14 days prior to the event. Cancellations must be received no later than 14 days prior to the event to receive a 50% refund of the registration fee. No refunds will be issued if cancellation is received less than 14 days prior to the event. Advanced payment in full is required to reserve your space at this event. Attendee lists will be filled on a first-come, first serve basis in the order that payment is received. Fax, , or phone your credit card information with registration. Tel: (719) Fax: (719) Visa MasterCard American Express Discover workshop.na@ adinstruments.com Cardholder s name: Card number: Exp date: Credit card billing address:
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