Prezentačný deň vedy Výskumného centra AgroBioTech
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1 Prezentačný deň vedy Výskumného centra AgroBioTech Nitra,
2 Laboratórium mikroskopických analýz Možnosti využitia pre riešenie základných a aplikovaných vedecko-výskumných projektov Ing. Jaromír Vašíček PhD., Ing. Lenka Kuželová, PhD. Nitra,
3 Výskumné zameranie laboratória Živočíšne genetické zdroje (čerstvé a mrazené vzorky): Analýza kvality ejakulátu hospodárskych zvierat Analýza kvality embryí hospodárskych zvierat Analýza kvality dospelých kmeňových buniek hospodárskych zvierat Plánujeme tiež analýzy rastlinných a mikrobiálnych vzoriek a analýzy pre potravinársky priemysel
4 Prístrojové vybavenie laboratória: Ultramikrotóm LEICA EM UC7 Kryotóm LEICA CM 1950 Laboratórny digestor 2, typ DS1 Optický svetelný mikroskop NICON ECLIPSE E100 Invertný fluorescenčný mikroskop LEICA DMI 6000B s kamerou DCF 345 FX Transmisný elektrónový mikroskop JEM-2100 JEOL
5 Prístrojové vybavenie laboratória: Ultramikrotóm Kryotóm
6 Prístrojové vybavenie laboratória: Invertný fluorescenčný mikroskop
7 Prístrojové vybavenie laboratória: Transmisný elektrónový mikroskop
8 Prístrojové vybavenie laboratória živočíšnych biotechnológií zameraného na bunkové kultúry (prof. Chrenek): Invertný svetelný mikroskop LEICA DM IL LED s kamerou EC3 Optický svetelný mikroskop NICON ECLIPSE E100 CO 2 inkubátor INCO 108 MEMMERT Termostat IN55 MEMMERT Vodný kúpeľ JULABO 13 Laminárny box Labculture Class II ESCO
9 Prístrojové vybavenie laboratória živočíšnych biotechnológií zameraného na bunkové kultúry (prof. Chrenek): Invertný svetelný mikroskop
10 Bunkové kultúry APVV , VEGA 1/0611/15, KEGA 011 UPJŠ-4/2014 APVV Kryouchovávanie živočíšnych genetických zdrojov na Slovensku (prof. Chrenek) Izolácia, kultivácia, kryouchovávanie a hodnotenie kmeňových buniek hydiny: a) embryonálne kmeňové bunky (ESC Embryonal Stem Cells) - izolácia zo zárodočného terčíka žĺtka čerstvo znesených vajec b) primordiálne zárodočné bunky (PGCs - Primordial Germ Cells) izolácia z krvi 2,5-3 dňového embrya z gonád 5-7 dňového embrya
11 Fluorescenčná mikroskopia: Princíp fluorescenčnej mikroskopie okulár dichroické zrkadlo nm 490 nm 525 nm barierový filter zdroj svetla excitačný filter dichroické zrkadlo objektív vzorka
12 Využitie FM: Fluorescenčná mikroskopia: bunková biológia - identifikácia bunkových organél, - zviditeľnenie niektorých bunkových štruktúr (jadro, cytoskelet) - sledovanie biochemických dejov - diagnostika a lokalizácia poškodenia buniek, tkanív, orgánov. mikrobiológia - identifikácia rôznych bakteriálnych rodov
13 Fluorescenčná mikroskopia
14 Fluorescenčná mikroskopia: Embryo králika DAPI PI Yo-Pro-1 Spermie králika
15 Transmisná elektrónová mikroskopia:
16 Transmisná elektrónová mikroskopia: zdroj vysokého napätia (200 kv) preparáty pre TEM o hrúbke nm až x zväčšenie STEM: x ultraštruktúra biologických vzoriek (rastlinných, či živočíšnych tkanív resp. buniek, rôznych mikroorganizmov, baktérií a vírusov a pod.)
17 Transmisná elektrónová mikroskopia: Grades of acrosome morphology of rabbit spermatozoa (membrane status) x x x x grade I sperm with intact plasma membrane of the head and intact acrosome grade II sperm with waved plasma membrane grade III sperm with swollen or damaged acrosome grade IV sperm with pseudo acrosomal reaction formed by vesicles and with loss of acrosomal content Vasicek J., Pivko J., Chrenek P.: Reproductive performance of New Zealand White rabbits after depletion of apoptotic spermatozoa. Folia Biologica (Krakow), 62, (2), 2014,
18 Transmisná elektrónová mikroskopia: Cattle Embryo 200X
19 Transmisná elektrónová mikroskopia: Rabbit Embryonic Stem Cells 7 200x Fibroblast and resc 4 800x
20 Imunoelektrónová mikroskopia: LM IM-TEM Fluor/Confocal
21 Elektrónová tomografia:
22 TEM a rastlinné vzorky:
23 Ebola TEM a mikrobiálne vzorky: Rod-shaped bacterium
24 Green (young) cheese - thin section TEM a potraviny: Ice-cream (Lactococcus lactis subsp. cremoris KB) location in full-fat Cheddar cheese
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