Adel R Moawad, Ph D, MVSc, DVM

Transcription

1 Adel R Moawad, Ph D, MVSc, DVM Post Doctoral Fellow, Department of Obstetrics and Gynecology, McGill University Contact details Address: Urology Research Laboratory, H6-19, Royal Victoria Hospital, McGill University, 687, Pins Avenue, West, Montreal, Quebec, H3A 1A1, Canada Phone: adelreda902@hotmail.com, adel.abouelmagd@mail.mcgill.ca, adel.moawad@cu.edu.eg Education December 2010: Degree of Doctor of Philosophy (Ph D), Division of Animal Sciences, School of Biosciences, Faculty of Sciences, University of Nottingham, UK. Under supervision of Professor Keith Campbell (who did the first cloned animal Dolly the sheep). Thesis entitled (Cryopreservation of ovine oocytes). March 2005: Master of Veterinary Medical Sciences (MVSc.) Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Egypt. Under supervision of Professor Abou Baker EL-Wishy. Thesis entitled (In vitro maturation and fertilization of camel oocytes). September 2000: Bachelor Degree in Veterinary Medical Sciences (DVM) Faculty of Veterinary Medicine, Cairo University, Egypt, Very Good (Honors). Academic positions April 2012 Present: Post Doctoral Fellow, Faculty of Medicine, McGill University, Montreal, Quebec, Canada. January 2011 Present: Lecturer of Animal Reproduction, Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University. September 2006 December 2010: Ph D researcher, Division of Animal Sciences, School of Biosciences, University of Nottingham. March 2005 December 2010: Assistant Lecturer of Animal Reproduction, Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University. November 2000 to March 2005: Demonstrator of Animal Reproduction, Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University. 2008, 2009, 2010: Teaching assistant, Division of Animal Sciences, University of Nottingham. Research Over the last fourteen years my research has been focused on different aspects of assisted reproductive technologies such as oocyte collection, evaluation, in vitro maturation (IVM), in vitro fertilization (IVF) using epididymal and frozen spermatozoa, parthenogenetic activation, somatic cell nuclear transfer (SCNT), Intracytoplasmic Sperm Injection (ICSI), embryo culture and 1

2 embryo evaluation in different mammalian species include cattle, camel, buffalo, sheep, goat, pigs, mice and rats and humans. Also my research focused on cryopreservation of oocytes at different stage (germinal vesicle (GV) or metaphase II (MII)) in cattle, sheep, mice and rats using different techniques such as slow freezing or vitrification by Cryoloop, solid surface vitrification (SSV) or open pulled straw (OPS) trying to establish an oocyte and embryo banks for widespread application of ART in different species. I studied the effect of different vitrification techniques of immature oocytes on viability, in vitro maturation (nuclear and cytoplasmic maturation), MPF/MAPK activities, sperm penetration, in vitro fertilization, in vitro embryo development following IVF and SCNT, embryo evaluation (morphological or by different stains such as Hoechst stain and TUNEL assay for total cell numbers and apoptosis in blastocyst stage embryos) or using karyotyping technique. In addition to evaluation the effects of vitrification on oocyte spindle and chromosome configuration using immunostaining technique, fluorescence microscopy and image analysis system. I am also interested in molecular biology, cell biology and tissue culture. I have been worked with embryo cryopreservation in cattle, sheep, mice, rats and human. Current Research My current research focuses on improvement the efficiency of oocyte cryopreservation as a tool for fertility preservation in women and for genetic preservation and wide spread application of ART in animals by using mouse model. The goal of my studies is to evaluate the effect of L-carnitine (known to play an essential role in fat metabolism and energy production, and also acts as an antioxidant) supplementation during vitrification and IVM on viability, nuclear maturation, spindle configuration, ATP levels, mitochondrial activity and subsequent development of mouse oocytes vitrified at GV-stage. Publications 2014 Mohamed Fathi, Adel A. Seida, Refaat R. Sobhy, Gamal M. Darwish, Magdy R. Badr, Adel R. Moawad (2014): Caffeine supplementation during IVM improves frequencies of nuclear maturation and pre-implantation development of dromedary camel oocytes following IVF. Theriogenology (in press) Adel R Moawad, Seang Lin Tan, Baozeng Xu, Chen HY, Teruko Taketo. (2013). L-carnitine supplementation during vitrification of mouse oocytes at the germinal vesicle stage improves preimplantation development following maturation and fertilization in vitro. Biology of Reproduction, 88, 104, 1-8. Adel R Moawad, Jie Zhu, Inchul Choi, Dasari Amarnath, Wenchao Chen, Keith H Campbell. (2013). Production of good-quality blastocyst embryos following IVF of ovine oocytes vitrified at the germinal vesicle stage using a cryoloop. Reproduction, Fertility and Development, 25: Adel R. Moawad, Jie Zhu, Inchul Choi, Dasari Amarnath, Keith H. S. Campbell (2013). Effect of cytochalasin B pre-treatment on viability, frequencies of in vitro fertilization and subsequent development of ovine oocytes vitrified at germinal vesicle stage. CryoLetters, 34:

3 EL-Shahat, KH., Abo-El maaty, AM., Moawad, AR. (2013). Follicular fluid composition in relation to follicular size in pregnant and non-pregnant dromedary camels (Camelus dromedaries). Animal Reproduction, 10: Adel R. Moawad, Jie Zhu, Inchul Choi, Pat Fisher, Zsuzsanna Polgar, Andras Dinnyes, Keith H.S. Campbell (2012). In vitro fertilization of ovine oocytes vitrified by solid surface vitrification (SSV) at germinal vesicle stage. Cryobiology, 65: Adel R. Moawad, Inchul Choi, Jie Zhu, and Keith H.S. Campbell (2011). Ovine oocytes vitrified at germinal vesicle stages as recipient cytoplasts for somatic cell nuclear transfer (SCNT). Cellular Reprogramming, 13: Dasari Amarnath, Inchul Choi, Adel R Moawad, Teruhiko Wakayama and Keith H S Campbell (2011): Nuclear-cytoplasmic incompatibility and inefficient development of pig-mouse cytoplasmic hybrid embryos. Reproduction, 142: Dasari Amarnath, Sayaka Wakayama, Jie Zhu, Adel R. Moawad, Teruhiko Wakayama, Keith H. S. Campbell (2011): The novel use of modified pig zygotic medium for the efficient culture of the preimplantation mouse embryos. Theriogenology, 76: Conference proceeding publications Moawad AR., Xu B., Tan., SL., Taketo T. (2014): Impact of L-carnitine supplementation during vitrification on mitochondrial distribution and ATP levels in the mouse oocytes vitrified at the germinal vesicle stage, thawed and then matured in vitro. SSR annual meeting (submitted). Moawad AR, Tan SL, Xu B, Chen HY, Taketo T. (2013). L-carnitine supplementation during vitrification of mouse oocytes at the germinal vesicle stage improves spindle configuration and subsequent pre-implantation development following maturation and fertilization in vitro. Society for the Study of Reproduction (SSR) annual meeting (Abstract No. 697). Moawad, AR, Darwish, GM., Badr, MR. and EL-Wishy, AB. (2012): In vitro fertilization of dromedary camel (Camelus dromedaries) oocytes with epididymal spermatozoa. Reproduction Fertility and Development 24: Moawad, AR, Zhu, J., Choi, I., and Campbell, KHS. (2011): Developmental competence of ovine oocytes vitrified at germinal vesicle stage: In vitro fertilization, parthenogenetic activation and somatic cell nuclear transfer (SCNT). Reproduction, Fertility and Development, 23: 145. Moawad, AR., Zhu, J., Choi, I., and Campbell, KHS. (2010): Effect of demecolcine pre-treatment on viability, timing of first polar body extrusion, spindle configuration and subsequent development of ovine oocytes vitrified at germinal vesicle (GV) stage. Reproduction, Fertility and Development 22,

4 Moawad, AR., Zhu, J., Choi, I., and Campbell, KHS. (2010): Developmental competence of ovine oocytes vitrified at germinal vesicle stage by two different protocols, Solid Surface Vitrification (SSV) or Cryoloop. Cryobiology, 61: 37. Moawad, AR., Choi, I., Zhu, J., and Campbell, KHS. (2010): Ovine oocytes vitrified at germinal vesicle stage as recipient cytoplasts for Somatic Cell Nuclear Transfer (SCNT). Society for Reproduction and Fertility Annual Meeting, 2010, Nottingham, UK. Moawad, A.R.; Choi, I.; Zhu, J., and K.H.S. Campbell. (2009): Vitrification of immature ovine oocytes with cryoloop: Effect of cytochalasin B pretreatment on subsequent development. Reproduction, Fertility and Development, 21: 135. Moawad AR.; Choi, I.; Zhu, J.; Amarnath, D., Campbell, KHS. (2009): Spindle configuration and in vitro embryo development of ovine oocytes vitrified at germinal vesicle stage: Effect of caffeine treatment on subsequent development. Cryobiology, 59: 376. Moawad, AR.; Zhu, J.; Choi, I., and Campbell, KHS. (2009): Parthenogenetic activation of ovine oocytes vitrified at germinal vesicle stage. Proceeding of The Society for Reproduction and Fertility Conference, July, Oxford, UK, O7. Moawad, AR., and Campbell, KHS. (2008): Vitrification of immature ovine oocytes with cryoloop: Effect on meiotic maturation. Reproduction Fertility and Development, 20: 122. Moawad, A R.; Amarnath, D.; Choi, I.; Fisher, P.; Zhu, J., and Campbell, KHS. (2008): Vitrification of immature ovine oocytes with cryoloop: Effect on in vitro fertilization rates Cryobiology, 57:323. Moawad, AR., Fisher, P.; Polgar, Z.; Dinnyes, A., and Campbell, K.H.S. (2008): In vitro fertilization of ovine oocytes vitrified by Solid Surface Vitrification (SSV) at germinal vesicle stage. Proceeding of The society for Reproduction and Fertility Conference, P40. Presentations Invited speaker, Division of Urology, McGill University, Montreal, Quebec (December, 2013, title: Oocyte cryopreservation: applications, challenges and progresses). Poster presentation, Society for the Study of Reproduction (SSR) annual conference, Montreal, Quebec, (July, 2013), (poster No. 697). Platform presentation, Centre for the Study of Reproduction at McGill Research, Day, Montreal, Quebec, Canada, (May, 2013). Invited speaker at The Department of Animal Sciences, Food and Nutrition, Southern Illinois University, Carbondale, USA (January, 2012, title: Cryopreservation of ovine oocytes). 4

5 Poster presentation at 38 th Annual conference of International Embryo Transfer Society, Phoenix, Arizona, (January, 2012). Platform and Poster Presentations at 37 th Annual conference of International Embryo Transfer Society, Orlando, Florida, USA (January, 2011). Poster presentation at The Society for Reproduction and Fertility Annual Conference Nottingham, UK (2010). Platform presentation at 46 th International Conference of Society for Cryobiology, Bristol, UK (2010). Poster presentation 36 th Annual Conference of International Embryo Transfer society, Cordoba, Argentina (2010). Platform presentation at 45 th International Conference of Society for Cryobiology, Sapporo, Japan (2009). Platform presentation at The Society for Reproduction and Fertility Annual Conference Oxford, UK (2009). Poster presentation 35 th Annual Conference of International Embryo Transfer society, San Diego, California (2009). Platform presentation at 44th International Conference of Society for Cryobiology, Charlotte, North Carolina, USA (2008). Poster presentation at The Society for Reproduction and Fertility Annual Conference Edinburgh, UK (2008). Poster presentation at 34 th Annual Conference of International Embryo Transfer Society, Denver, Colorado, USA (2008). Poster presentation at The Annual Post Graduate Conference at Division of Animal Sciences, University of Nottingham (2008). Platform presentation at The Annual Post Graduate Conference at Division of Animal Sciences, University of Nottingham (2007). Conferences Society for the Study of Reproduction (SSR) 46 th Annual Meeting, Montreal, Quebec, Canada, (July 22-26, 2013). Centre for the Study of Reproduction at McGill Research, Day, Montreal, Quebec, Canada, (May, 2013). Centre for the Study of Reproduction at McGill Research, Day, Montreal, Quebec, Canada, (May, 2012). 23 nd Annual McGill Urology Research Day, McGill University, Montreal, Quebec, Canada (April, 2012). 22 nd Annual McGill Urology Research Day, McGill University, Montreal, Quebec, Canada (April, 2012). 38 th Annual conference of International Embryo Transfer Society, Phoenix, Arizona, USA (2012). 37 th Annual conference of International Embryo Transfer Society, Orlando, Florida, USA (2011). 46 th International Conference of Society for Cryobiology, Bristol, UK (2010). 5

6 The Society for Reproduction and Fertility Conference, Nottingham, UK (2010). 36 th Annual Conference of International Embryo Transfer society, Cordoba, Argentina (2010). 45 th International Conference of Society for Cryobiology, Sapporo, Japan (2009). The Society for Reproduction and Fertility Conference, Oxford, UK (2009). 35 th Annual Conference of International Embryo Transfer society, San Diego, California (2009). Nottingham Cattle Fertility Conference, The University of Nottingham, UK (2008). 44 th International Conference of Society for Cryobiology, Charlotte, North Carolina, USA (2008). The Society for Reproduction and Fertility Conference Edinburgh, UK (2008). 34 th Annual Conference of International Embryo Transfer Society, Denver, Colorado, USA (2008). Academic Teaching Group teaching. One to one tutoring. Demonstration. Workshop. Designing Veterinary Reproduction and Reproductive Biology courses (Reproductive physiology, Endocrinology, Gynecology in farm animals, Andrology and Artificial insemination in farm animals, Obstetrics in farm animals, Theriogenology in pets), Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University. Teaching and Lecturing of animal reproduction courses (Gynecology, Obstetric, Andrology and Artificial Insemination) in Different species such as Cattle, Buffalo, Sheep, Goat, Equine, Pigs, Camel, Dogs and Cats at Theriogenology Department, Faculty of Veterinary Medicine, Cairo University for 4 th and 5 th year undergraduate students. Using appropriate technology such as audio visual aids, and different programs (Virtual Learning environment) to support learning and teaching and to motivate and inspire students. Contributing to module development in reproductive biology, Faculty of Veterinary Medicine, Cairo University. Contributing in marking/assessing assignments and examinations (Practical, Written, Platform) in Animal Reproduction for 4 th and 5 th years undergraduate student, Faculty of Veterinary Medicine, Cairo University. Contributing in teaching of summer courses and clinical training of different aspects of Animal Reproduction (such as physiology of reproduction in male and female, pregnancy diagnosis, treatment of infertility, treatment of dystocia (difficult birth) using different approaches, semen collection, evaluation and artificial insemination, embryo transfer, in different species such as cattle, buffaloes, equine, camels, sheep, goat, dogs and cats) for undergraduate students, Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University. Design and Teaching of Animal Reproduction, Reproductive Biology and Reproductive Physiology Courses (Male and female) for Postgraduate Students (Master, Ph D students), Faculty of Veterinary Medicine, Cairo University. 6

7 Co-supervision of postgraduate students (master and Ph D) Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University. Responsible for Animal reproduction training program for Veterinarian in Ministry of Agriculture, Egypt. Member of Postgraduate studies board in Faculty of Veterinary Medicine, Cairo University. Teaching practical courses in different aspects of animal physiology (Artificial Insemination, Placentaion, Female Reproduction, Endocrinology, Nervous System, and Cardiovascular System) for undergraduate students in Division of Animal Sciences, School of Bioscience, University of Nottingham. Clinical Experiences Human Clinical Embryology April 2011-March Embryologist at Dar Elteb Hospital, Cairo, Egypt. April present. Embryologist, andrologist, OrigenElle Fertility Clinic and Women Health Centre (originally, Montreal Reproductive Centre), Montreal, Canada. Veterinary Reproduction Veterinarian in the clinic of Theriogenology Department, Faculty of Veterinary Medicine, Cairo University and private clinics and farms since November Treatments of all forms of infertility in cattle, buffalo, horses, sheep, goat, dog and cat. Estrous synchronization, Perform embryo transfer in cattle, buffalo, horses, sheep and goat. Perform pregnancy diagnosis in farm animals and pet animals by using different methods such as rectal palpation or ultrasound. Treatment of all forms of dystocia in cattle, buffalo, horses, camels, sheep, goat, dogs and cats using different methods including caesarian section. Semen collection, evaluation, and artificial insemination in cattle, buffalo, horses, camels, sheep, goat, dogs and cats. Training Courses April 2007: Training course in Birmingham Women s Hospital on Cryopreservation of human oocytes and embryos. September 2007: Training course on Cryopreservation of mouse oocytes and embryos using Solid Surface vitrification technique (SSV) and VS3a technique and Slow Freezing in Agriculture Biotechnology Center (Godollo, Hungry) with Professor Andres Dinnyes. Development and professional Skills Workshops From 2007 till 2010 attending the following workshops in Graduate School, The University of Nottingham, Planning your research, Getting into the habit of writing, How to prepare an effective poster presentation, Getting going on your thesis and getting your work published, Further presentation skills for researchers, MS Excel functionality b: data exploration + analysis and graphing, 7

8 MS PowerPoint: developing a professional presentation, Creating and managing long documents in word 2007, Nature of the doctorate and supervision process Computing and molecular biology, IT training support drop-in sessions, Essential information skills for new researchers in biosciences and Veterinary Medicine, Post-genomic and bioinformatics, Getting started with research design and statistics, Introduction to qualitative research Exploiting the power of MS Word a and b: long documents and paper. October Scientific writing workshop in Montreal Reproductive Centre, Montreal presented by Professor Gedis Grudzinskas; Editor; Reproduction Biomedicine Online, February Presentation skills and Networking skills workshops, Mitacs Step, Montreal, Canada Awards Mitacs Elevate Post Doctoral Fellowship, Montreal Canada, 2013 Invention application, McGill University, 2013 Post Doctoral Fellowship, McGill University, Montreal, Canada, 2012, 2013 International Publication Award, Cairo University, 2012, 2013, 2014 Travel Grant from Cairo University, 2012 Travel Grant from Cairo University, 2011 Travel Grant from Egyptian Cultural Bureau, London, 2009 Travel Grant from Society of Low Temperature Biology, 2008 Travel Grant from Society for Cryobiology, 2008 Travel Grant from BSDB, 2008, 2009 Honor Fell Travel Grant from BSCB, 2008 and 2009, 2010 Travel Grants, Society for Reproduction and Fertility, 2007, 2009 Ph D Scholarship from Ministry of Higher Education, Egyptian Government, 2006 Assistant Lecturer award, Faculty of Veterinary Medicine, Cairo University, 2006 ICDL (International Computer Driving License), 2006, Cairo University, Egypt Demonstrator Award, Cairo University Staff Club, 2003 Reviewer in Cellular Reprogramming, Reproduction Fertility and Development, Reproduction Journals Membership Society for the study of Reproduction (SSR) Society for Reproduction and Fertility (SRF) Society for Cryobiology (SFC) Society for Low Temperature Biology (SLTB) The British Society for Cell Biology (BSCB) The British Society of Developmental Biology (BSDB) International Society for Embryo Transfer (IETS) 8

9 The physiological Society Egyptian Society for Reproduction and Fertility Egyptian Society of Animal Management Language Arabic: Mother Language English: Very good writing and Speaking (International TOEFL Test, 2006, and IELTS Test, 2012) French: Basics References Dr. Dylan Sweetman, Ph D Lecturer of Molecular Embryology, Division of Animal Sciences, School of Biosciences, University of Nottingham, UK. Tel: + 44 (0) , E. mail: dylan.sweetman@nottingham.ac.uk Professor, Andras Dinnyes, Ph D Professor of Molecular Animal Biotechnology, Szent Istvan University, Hungary Tel: , E.mail: andras.dinnyes@biotalentum.hu Professor, Abou Baker EL-Wishy, Ph D, BVSc Professor of Animal Reproduction, Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Egypt. Tel.: +2 (0) , e mail: dr.bakrelwishy@gmail.com Dr. Inchul Choi, Ph D Assistant Professor, Department of animal biosystem sciences, College of Agriculture and Life Sciences Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, Republic of Korea Office: , fax: , icchoi@cnu.ac.kr Dr. Jie Zhu, Ph D Department of Biological resources, MRC Harwell, Mary Lyon Centre, Harwell Science and Innovation Campus, Oxfordshire, OX11 0RD, UK. E mail: j.zhu@har.mrc.ac.uk 9