43 (2) : , 1997 A cta Zoologica S inica

Transcription

1 43 (2) : , 1997 A cta Zoologica S inica (, ) ( ),,,,,,,,,, A B (Deurs et al., 1973 ; Grier, 1973 ; Todd, 1976 ; Gardiner, 1978 ; Poirier et al., 1982 ; Billard, 1986 ;, 1990 ;, 1992 ;, 1996a, 1996b ), Pseudosciaena crocea (Richardson) (, 1991, 1992), 1, 5,, 2ml,, 215 % (p H 714),, 111,, Ehrlich (, 1993) 24,, OL YMPUS BH r/ m 5,, ,

2 %, %,, 30, 215 %, 4 011mol/ L PBS, 1 %,, Epon 812, J EOL, J EM EX 113,, 2 % 3, 2 ( ) ( 1) (centriolar complex) (sleeve), ( 1) 211,, ( 1 B ; 2, 3, 4) 21111,,, ( 1 B ; 2),, ( 1 C H ; 5 10 ; 11),, ( 1 D F ; 6 9) ( 1 B H ; 2, 4 10 ; 11) 21112, ( 1 B ; 2) ( ), ( 1 B ; 2),, ( 1 D ; 6), ( 7),, ( 1 E ; 8) 21113, ( 1 B ; 2), 6 ( 2, 3 ; 13),, ; ( )

3 2 : Fig. 1 Diagram of the spermatozoon of the teleost, Pseudosciaena crocea ( Richardson) A. (appearance of a spermatozoon) B. (sagittal section of the sperm head) J. B (c j) [ represent the cross sections at the various levels (c j) indicated in B] K. (structure of the principal piece and the end piece of the tail ) (cross section of the principal piece) N O. (cross sections of the end piece) BB : (basal body) basal body and the initial part of the axoneme) C L. M. (cross section of the basket2like structure) C : (circular structure at the posterier end of the EP : (end piece of the tail) F : (flagellum)

4 H : (head of the sperm) (deeper area of the implantation fossa) IA : (initial part of the axoneme) (membrane parallel to the inner membrane of the sleeve) (microtubule) brane of the sleeve) (principal piece) N : (nucleus) PC : (proximal centriole) IM : (inner membrane of the sleeve) NM : (nuclear membrane) S : (central space of the sleeve) IF : m : M : (mitochondrion) MT : OM : (outer mem2 PM : (plasma membrane) V : (vesicle) PP :, ( 1 B, G J ; 2, 3, 10 ; 11 14) ( 1 ; 2, 3, 6 10 ; 11 14) 212 ( 1), 21211,,, ( 2, 3 ; 12, 21), 9 A B, ( 1 B, F, G; 2, 9, 10) ( 1 H J ; 11, 13, 14, 16) ( ) : 9 ( basket2like st ruc2 ture), ( 1 K, M ; 17, 18, 21, 22) ( 1 K, N, O ; 19, 20, 23) ( Fawcett, 1975 ;, 1992a, 1993) : (midpiece, ) ; ;,,

5 2 : 123 ( 2),, 312,, ( Grier, 1973 ; Deurs et al., 1973 ; Gar2 diner, 1978 ; Poirier et al., 1982 ;, 1996a, 1996b),,, ( 24a),, ( 24b), 313, 9, 9,,, (, 1992b) 9 + 2,, ( 9, 10) ( 1 F ; 9) ( 1 E ; 8), ( 1 G; 10) 314,, (lipid bilayer) (, 1996a, 1996b) 315,

6 124 43, ;,, ( 1 K, L, N, O ; 15, 16, 19, 20, 23), ( 17, 18), 1992a. 25 (4) : , 1992b. 11 (4) : , (4) : , 1996a. 29 (3) : , 1996b. 17 (4) : , (4) : ,,,, ( ) 7 (3) : ,,,, ( ) 8 (3) : :.,, (3) : Billard, R Spermatogenesis and spermatology of some teleost fish species. Reprod. N ut r. Develop. 26 (4) : Deurs, B. van and U. Lastein 1973 Ultrasturcture of the spermatozoa of the teleost Pantodon buchholzi Peters, with particular reference to the midpiece. J. Ult rast ruct. Res. 42 : Fawcett, D. W The mammalian spermatozoon. Gardiner, D. M. Biol. 13 : Develop. Biol. 44 : Fine structure of the spermatozoon of the viviparous teleost, Cymatogaster aggregata. J. Fish Grier, H. J Ultrastructure of the testis in the teleost Poecilia latipi nna. J. Ult rast ruct. Res. 45 : Poirier, G. R. and N. Nicholson 1982 Fine structure of the testicular spermatozoa from the channel catfish Ictal urus punctat us. J. Ult rast ruct. Res. 80 : Todd, P. R Ultrastructure of the spermatozoa and spermiogenesis in New Zealand freshwater eels (Anguilli2 dae). Cell Tiss. Res. 171 : ( Abstract) THE UL TRASTRUCTURE OF THE SPERMATOZOON OF THE TEL EOST, PS EUDOSCIA ENA CROCEA ( RICHARDSON) YOU Yong2Long L IN Dan2J un ( Bioengi neeri ng College, Fujian Normal U niversity, Fuz hou , Chi na) The spermatozoon of t he teleost, Pseudosciaena crocea ( Richardson) consist s of two parts : the head and the tail (flagellum). The structure of the head is quite special. A large

7 2 : 125 nucleus is located at the ventral part of the head. The dorsal part of the head is occupied by the centriolar complex. The posterior end of the head is the sleeve. The ventral surface of the nucleus is convex, whereas the dorsal surface of the nucleus is concave. Within the nu2 cleus t he chromatin condenses into wads, among which are loose chromatin fibers. The im2 plantation fossa is on t he dorsal surface of t he nucleus. It is groove2like running f rom t he an2 terior end to the posterior end of the nucleus. The centriolar complex is in the implantation fossa. The proximal cent riole is perpendicular to t he major axis of t he spermatozoon, where2 as the basal body is parallel to the major axis of the spermatozoon. The anterior end of the basal body is roll2like. It is electron2dense, with some microtubules in it. The posterior end of the basal body splits into 9 strips. The sleeve contains several mitochondria and vesicles. Within the cytoplasm of the sleeve, there is a membrane which is near and parallel to, as well as thinner than the inner membrane of the sleeve. The tail, which is thin and long, stretches out of the central space of the sleeve. It consists of a principal piece and an end piece. The cent ral st ruct ure of t he principal piece is axoneme. There is only a little cytoplasm outside the axoneme. The 9 doublets of the axoneme is connected to the 9 strips of the poste2 rior end of the basal body. The initial part of the axoneme is special in structure : there is no central pair of the single microtubule, the doublets of it are electron2dense, and there are no dynein arms on the doublets. At the end of the principal piece, the microtubules, which con2 stitute the axoneme, spiral to become a basket2like structure. Several microtubules extend into the end piece of the tail. Key words Pseudosciaena crocea ( Richardson), Spermatozoon, Ultrastructure, Implan2 tation fossa, Centriolar complex, Basket2like structure ( Plate ) 1. ( The appearance of the spermatozoon under the light microscope) (A sagittal section of the sperm head) (A frontal section of the dorsal part of the sperm head) (A frontal section of the ventral part of the sperm head) (A cross section of the sperm head, showing the proximal centriole at the implantation fossa) 6. (A cross section of the sperm head, showing the anterior end of the basal body at the implantation fossa) 7., (A cross section of the sperm head, showing the basal body splitting into several strips) 8., (A cross section of the sperm head, showing the posterior end of the basal body splitting into 9 strips. At the inner side of the 9 strips, there is a circular structure)

8 (A cross section of the sperm head, showing the initial part of the axoneme at the implantation fossa, where there is no central pair of the single microtubule. At the inner side of the doublets, there is a circular structure. ) 10., (A cross section of the sperm head, showing the initial part of the flagellum, where there is no central pair of the single microtubule in the axoneme) ( Plate ) 11. (A cross section of the sperm head, showing the flagellum in the central space of the sleeve) 12. (A sagittal section of the sperm head, showing the membrane in the sleeve parallel to the inner membrane of the sleeve) 13. (A cross section of the sleeve) (A cross section of the posterior end of the sleeve) 15. (A transmission electron micrograph of the end part of the tail, showing the axoneme and basket - like structure in the principal piece and the microtubules in the end piece) (A cross section of the principal piece) ( Cross sections through the basket2like struc2 ture of the principal piece. The central pair of single microtubule may or may not be seen) (Cross sections through the end piece of the tail) , (A longitudinal section of the principal piece, showing the basket2like structure) (A longitudinal section through the basket2like structure) (A longitudinal section of the end piece) (24a) (24b) [ Longitudinal sections of the sperm heads of the teleosts, Pseu2 dobagrus f ulvi draco (Richardson) (24a) and Cypri nus carpio (Linnaeus) (24b) ] A : (axoneme) of the tail) BB : (basal body) IA : (initial part of the axoneme) (inner membrane of the sleeve) OM : (outer membrane of the sleeve) the sleeve) V : (vesicle) membrane of the sleeve) BS : (basket2like structure) F : (flagellum) EP : (end piece IB : (intercentriolar body) M : (mitochondrion) PC : (proximal centriole) IM : N : (nucleus) S : (central space of (arrow indicates the membrane parallel to the inner