Characters and character states for Nuclear Division/Spindle Pole Body

Transcription

1 Characters and character states for Nuclear Division/Spindle Pole Body Locations for Nuclear Division/Spindle Pole Body NucDiv/SPB : ME - meiosis MI - mitosis 1. Centriole 0, absent 2, present (CAM, centriolar associated material; Ce, centriole; MT, astral microtubules; NE, nuclear envelope; PF, polar fenestra; S, spindle) 2. Basic organization of the spindle pole 0, centriolar-associated material (CAM, centriolar associated material; Ce, centriole; MT, astral microtubules; NE, nuclear envelope; PF, polar fenestra; S, spindle)

2 1, spindle pole body consists of small amount of extranuclear material (ESPB, extranuclear spindle pole body; ISPB, intranuclear spindle pole body; NE, nuclear envelope; S, spindle) 2, ring-like spindle pole body (CS, cross section; NE, nuclear envelope; R, ring; S, spindle) 3, spindle pole body a plaque or disc (CS, cross section; Di, disc; ISPB, intranuclear spindle pole body; NE, nuclear envelope; Pl, plaque; S, spindle)

3 4, spindle pole body globular (DC, electron dense core; NE, nuclear envelope; S, spindle; SPB, spindle pole body) 3. Spindle pole body form, interphase-prophase: 0, quadrilaterial plaque (CS, cross section; IE, intranuclear element; NE, nuclear envelope; Pl, plaque) 1, unlayered disc (CS, cross section; Di, disc; IE, intranuclear element; NE, nuclear envelope)

4 2, a 2-layered disc (Di, disc; NE, nuclear envelope; IE, intranuclear element; L1, L2, layers 1, 2; S, spindle) 3, a 3-layered disc (L1, L2, L3, layers 1,2,3, MP, middle piece; NE, nuclear envelope) 4, a 7- to 9-layered disc (L1 L7, layers 1 7; NE, nuclear envelope)

5 5, a globoid (GE, globular element; IE, intranuclear element; MP, middle piece; NE, nuclear envelope) 6, a subgloboid with flat internalized layer (DL, flat electron dense layer; GE, globular element; MP, middle piece; NE, nuclear envelope) 7, a subgloboid with internalized layer convex with respect to the nucleus (DL, convex electron dense layer; GE, globular element; N, nucleus; NE, nuclear envelope; Nu, nucleolus)

6 8, notched ring with middle piece and intranuclear component (CS, cross section; HMP, half middle piece; ISPB, intranuclear spindle pole body; NE, nuclear envelope; S, spindle) 9, ring containing microtubules but lacking centriolar 9-fold symmetry (CS, cross section; R, ring; NE, nuclear envelope, S, spindle) a, slight amount of extranuclear material and intranuclear material on either side of nuclear envelope (ESPB, extranuclear spindle pole body; ISPB, intranuclear spindle pole body; NE, nuclear envelope; S, spindle)

7 b, centriolar-associated extranuclear and intranuclear components with intact nuclear envelope (Ce, centriole; CS, cross section; ESPB, extranuclear spindle pole body; ISPB, intranuclear spindle pole body; NE, nuclear envelope; S, spindle) c, centriole-associated material (CAM, centriolar associated material; Ce, centriole; MT, astral microtubules; NE, nuclear envelope; PF, polar fenestra; S, spindle) 4. Spindle pole body form, metaphase-anaphase: 0, quadilateral plaque with intact nuclear envelope and internal microtubule organizing center (Pl, plaque; CS, cross section; NE, nuclear envelope; INSPB, intranuclear spindle pole body; S, spindle)

8 1, unlayered disc with intact nuclear envelope and internal microtubule organizing center (CS, cross section; Di, disc; ISPB, intranuclear spindle pole body; NE, nuclear envelope; S, spindle) 2, a 2-layered disc with intact nuclear envelope and internal microtubule organizing center (Di, disc; ISPB, intranuclear spindle pole body; L1, L2, layers 1,2; NE, nuclear envelope; S, spindle) 3, a 4-layered disc directly connected to spindle (SMT, spindle microtubules; ne, nuclear envelope)

9 4, a 5-layered disc directly connected to spindle (Di, disc; L1 L5, layers 1 5; MC, membranous cap; NE, nuclear envelope; S, spindle) 5, a 7- to 9-layered disc directly connected to spindle (L1 L7, layers 1 7; NE, nuclear envelope; S, spindle) 6, an ellipsoid that expands by more than 100% of its interphase-prophase size directly connected to spindle (EDC, electron dense core; NE, nuclear envelope; S, spindle; SPB, spindle pole body)

10 7, a globoid that expands by more than 100% of its interphase-prophase size directly connected to spindle (C, cytoplasm; NE, nuclear envelope; S, spindle; SPB, spindle pole body with two globular elements and middle piece) 8, a globoid with limited enlargement directly connected to spindle (SPB, spindle pole body) Link to the article at SpringerLink from which the article is licensed: 2ku22p/ 9, a subgloboid with flat internalized layer directly connected to spindle (DC, electron dense core; MC, membranous cap; S, spindle; SPB, spindle pole body)

11 a, a subgloboid with internalized layer convex with respect to the spindle and directly connected to spindle (DL, electron dense layer; NE, nuclear envelope; S, spindle) b, notched ring with persistent half middle piece and clear zone between intranuclear component and nuclear envelope (CS, cross section; HMP, half middle piece; ISPB, intranuclear spindle pole body; NE, nuclear envelope; S, spindle) c, ring containing microtubules but lacking centriolar 9-fold symmetry (CS, cross section; NE, nuclear envelope; R, ring; S, spindle)

12 d, slight amount of extranuclear material and intranuclear material on either side of nuclear envelope (ESPB, extranuclear spindle pole body; ISPB, intranuclear spindle pole body; NE, nuclear envelope; S, spindle) e, centriolar-associated extranuclear and intranuclear components with intact nuclear envelope (Ce, centriole; CS, cross section; ESPB, extranuclear spindle pole body; ISPB, intranuclear spindle pole body; NE, nuclear envelope; S, spindle) f, centriole associated material (CAM, centriolar associated material; Ce, centriole; MT, astral microtubules; NE, nuclear envelope; PF, polar fenestra; S, spindle)

13 5. Spindle development site: 0, gap in nuclear envelope (CAM, centriolar associated material; Ce, centriole; MT, astral microtubules; NE, nuclear envelope; PF, polar fenestra; S, spindle) 1, cytoplasm (C, cytoplasm; MT, astral microtubules; NE, nuclear envelope; Nu, nucleus; SPB, spindle pole body) 2, intranuclear (nuclear envelope intact) (CS, cross section; HMP, half middle piece; ISPB, intranuclear spindle pole body; NE, nuclear envelope; S, spindle)

14 6. Spindle pole body migration (modified from Heath, 1980, Int. Rev. Cytol. 64:1): 0, migration before spindle formation 1, migration during spindle formation 2, formation of adjacent fanshaped arrays of microtubules that reorient during spindle formation

15 3, integration of spindle pole body into invaginated nuclear envelope before spindle formation (NE, nuclear envelope; S, spindle; SPB, spindle pole body) 7. Metaphase nuclear envelope: 0, intact (ESPB, extranuclear spindle pole body; ISPB, intranuclear spindle pole body; NE, nuclear envelope; S, spindle) 1, intact with small polar fenestrae plugged by the spindle pole bodies (Di, disc; MC, membranous cap; NE, nuclear envelope; S, spindle; PF, polar fenestrae)

16 2, loose polar fenestrae, including extensions of nuclear envelope into the cytoplasm at the spindle pole, but mainly intact (CAM, centriolar associated material; Ce, centriole; MT, astral microtubules; NE, nuclear envelope; PF, polar fenestra; S, spindle) 3, partially dispersed (C, cytoplasm; NE, nuclear envelope; S, spindle; SPB, spindle pole body) Link to the original article from which the image (right) is reproduced: 4, nearly or entirely dispersed (DC, electron dense core; MC, membranous cap; S, spindle; SPB, spindle pole body)

17 8. Telophase nuclear envelope: 0, retention of complete nuclear envelope around the chromatin with median constriction/fragmentation (C, cytoplasm; NE, nuclear envelope; S, spindle; SPB, spindle pole body) 1, retention of parts of a disrupted nuclear envelope around the chromatin (C, cytoplasm; NE, nuclear envelope; S, spindle; SPB, spindle pole body) Link to the original article from which the image (right) is reproduced: 2, new envelope forms within old envelope (C, cytoplasm; E2, inner membrane cisternae; NE (E1), original nuclear envelope; SPB, spindle pole body) 3, dispersed in interzone (C, cytoplasm; NE, nuclear envelope; S, spindle)

18 4, reappearance of nuclear envelope after metaphase (C, cytoplasm; NE, nuclear envelope; SPB, spindle pole body; S, spindle) 5, retention of the nuclear envelope around the chromatin with constriction/fragmentation near the poles, and interzone is cut off from the daughter nuclei (C, cytoplasm; NE, nuclear envelope; S, spindle) 9. Perinuclear endoplasmic reticulum: 0, absent 1, present (Ce, centriole; ER, endoplasmic reticulum; MT, microtubules; NE, nuclear envelope; S, spindle)

19 10. Spindle vesicles: 0, absent 1, present (NE, nuclear envelope; SPB, spindle pole body; S, spindle; V, vesicle) 11. Central spindle: 0, absent (NE, nuclear envelope; Ce, centriole; S, spindle; Ch, chromatin) 1, present (NE, nuclear envelope; CeS, outer limits of central spindle; Ch, chromosome) Link to the article at SpringerLink from which the article is licensed: qw3022/?p=f2b81777b65f4aaf97663edca79f9bb6& pi=3

20 12. Metaphase plate: 0, absent (NE, nuclear envelope; CeS, outer limits of central spindle; Ch, chromosome) Link to the article at SpringerLink from which the article is licensed: qw3022/?p=f2b81777b65f4aaf97663edca79f9bb6& pi=3 1, present (NE, nuclear envelope; Ce, centriole; S, spindle; Ch, chromatin) 13. Spindle pole body cap: 0, absent (DC, electron dense core; NE, nuclear envelope; S, spindle; SPB, spindle pole body)

21 1, continuous with the nuclear envelope (SPB, spindle pole body; DC, electrondense core; NE, nuclear envelope; S, spindle) Link to the original article from which the image (right) is reproduced: 2, distinct from nuclear envelope (Di, disc; MC, membranous cap; NE, nuclear envelope; S, spindle) 3; fragments of a discontinuous membrane that do not form a true cap (DC, electron dense core; MC, membranous cap; S, spindle; SPB, spindle pole body)

22 14. Interphase chromatin condensation: 0, absent 1, present (Cr, chromatin; Nu, nucleolus; SPB, spindle pole body; C, cytoplasm; NE, nuclear envelope) 15. Nucleolus behavior: 0, nucleolus is dispersed and no longer recognizable during prophase (dnu, disintegrating nucleolus; Ce, centriole; MT, astral microtubule; NE, nuclear envelope) 1, nucleolus discarded between prophase and metaphase (N, nucleus; NE, nuclear envelope; Nu, disintegrating nucleolus check spelling) Link to the original article from which the image (right) is reproduced:

23 2, nucleolus or part of it is more persistent and is discarded after metaphase (C, cytoplasm; Nu, nucleolus; SPB, spindle pole body; S, spindle; NE, nuclear envelope) 3, nucleolus is persistent throughout division (Nu, nucleolus; SPB, spindle pole body; S, spindle; NE, nuclear envelope) 16. Transient intranuclear element at late interphase-prophase: 0, absent (MP, middle piece; NE, nuclear envelope; L1, L2, L3, layers 1,2,3)

24 1, present within nucleus opposite the extranuclear spindle pole body (MP, middle piece; GE, globular element; NE, nuclear envelope; IE, intranuclear element)