Poultry Hydrolysates. enhance Stress Resistance in Pacific White Shrimp (Litopenaeus vannamei)

Transcription

1 Poultry Hydrolysates enhance Stress Resistance in Pacific White Shrimp (Litopenaeus vannamei) Presentation at the Asia Pacific Aquaculture Ho Chi Minh City, Vietnam December 10-13th, 2013 Dr. sc. agr. Franz-Peter Rebafka GePro Geflügel-Protein Vertriebsgesellschaft mbh & Co. KG GERMANY

2 Agenda Poultry Hydrolysates Enhance Stress Resistance in Pacific White Shrimp (Litopenaeus vannamei) GePro at a glance Stress in aquaculture Peptides as Immunomodulator Trial: Applied stress in shrimps

3 GePro at a Glance Part of a poultry integration

4 GePro at a Glance

5 Sea bass (the Mediterranean) Aquaculture: every 2 nd fish we eat Tilapia (Thailand) Barramundi (the Negev / Israel Salmon (Norway)

6 Aquaculture: ISSUE: High Stocking Density

7 High Stocking Density Thailand: 40 shrimps /m 2 to 160 shrimps/m 2 Infection rate: - In SE Asia 88% of shrimp are infected with one virus 53% have been infected with two to three viruses - EMS issue Poor Water quality (nitrite, ammonia, salinity, ) Reduced stress resistance Sensitivity and poor performance

8 Bioactive Peptides from Protein Hydrolysates Multiple functional properties Affecting Physiological and Metabolic Processes - Antibacterial peptides - Antioxidant peptides - ACE inhibitors (therapy for hypertension / heart failure) - Antidiabetic - Casomorphins (an opioid acting as a histamine releaser) (Lordan et al (2011) Marine bioactives as functional food ingredients) Affecting Immune Response

9 Objective of this Study: to verify Poultry Hydrolysates as Bioactive Peptides and Immuno Modulator increasing immune response in shrimps

10 Material & Methods Pacific White Shrimps (L. vannamei) Initial weight: 3.1 g/shrimp Stocking density: 70 shrimps/m 2 (aquarium) Water quality: brackish / 12 ppt salt Feeding: 3 x/day; to match 2.5% bw June 2012 Kasetsart University, Bangkok (Thailand); Dr Orapint Jintasataporn Dept. Of Aquaculture, Faculty of Fisheries Complete randomized design 4 replicates Significance test: Duncan s Multiple Range Test (p<0.05)

11 Test product: Enzymatically Hydrolyzed Poultry Peptides Origin: Poultry Bones / Carcasses Poultry Liver

12 PRODUCTION: Enzymatically Hydrolyzed Poultry Peptides 1. Heating (95 C) Coagulation 2.Tricanter Phase Separation Protein, Stickwater, Fat 3. + Enzymes Protein Hydrolysation 4. Separation Peptides (liquid) Bone Residues 5. Spray drying Peptides (dry); el..liver emb...meatbone

13 Test product: Enzymatically Hydrolyzed Poultry Peptides Main characteristic: Low in Molecular Weight

14 Experimental Feed: Control diet: Marine protein Test diets: Peptides to compensate for vegetable protein el: Enzymat. hydrolyzed poultry liver emb: Enzymat. hydrolyzed poultry bones

15 Blood Diagnostic for Immune Response Total Hemolymph Protein (TP) Hemolymph: shrimp blood, with - (oxy)-hemocyanin: O 2 -carrying molecules - (lectins):immunoreactive molecules The more TP / lectins, the better the immune competency Total Hemocyte Cell Count (THC) Primary (non-specific) defense in shrimp to inactivate foreign cells: - Phagocytosis - Encapsulation The more hemocyte, the better the immune competency

16 Feeding Peptides: Improved Performance and Less Mortality Mean value with different superscript are significantly different (p<0.05)

17 Feeding Peptides: More Hemolymph and Hemocytes No Stress TP: Total hemolymph protein (g/dl) THC: Total hemocyte count (x 10 6 ) MR: Mortality rate (%)) Mean value with different superscript are significantly different (p<0.05)

18 a) Salinity Stress (0-40 ppt) b) Chemical Stress NH 3+ (50 ppm, ph 7-8) NO 2 - (20 ppm) c) Biological Stress + White Spot Syndrome Virus (WSSV) + Vibrio harveyi (subcutaneous injection)

19 Salinity Stress Protocol a) Salinity Stress 0-4. week: 12 ppt salinity week: 20 ppt a1: week: 0 ppt (Low Salinity) a2: week: 40 ppt (High Salinity)

20 Feeding Peptides: More Hemocytes and Less Mortality With Stress TP: Total hemolymph protein (g/dl) THC: Total hemocyte count (x 10 6 ) MR: Mortality rate (%)) Man value with different superscript are significantly different (p<0.05)

21 Feeding Peptides: More Hemocytes With Stress TP: Total hemolymph protein (g/dl) THC: Total hemocyte count (x 10 6 ) MR: Mortality rate (%)) Man value with different superscript are significantly different (p<0.05)

22 Chemical Stress Protocol b) Chemical Stress 0-4. week: at 12 ppt salinity week: at 20 ppt b1: week: at 20 ppt / 50 ppm NH 3+ (ph 7-8) (Ammonia) b2: week: at 20 ppt / 20 ppm NO 2 - (Nitrite)

23 Feeding Peptides: More Hemolymph With Stress (normal range<0.5 ppm) TP: Total hemolymph protein (g/dl) THC: Total hemocyte count (x 10 6 ) MR: Mortality rate (%)) Man value with different superscript are significantly different (p<0.05)

24 Feeding Peptides: More Hemolymph and Hemocytes; less Mortality With Stress (normal range<1.0 ppm) TP: Total hemolymph protein (g/dl) THC: Total hemocyte count (x 10 6 ) MR: Mortality rate (%)) Man value with different superscript are significantly different (p<0.05)

25 Biological Stress Protocol c) Biological Stress 0-4. week: at 12 ppt salinity c1) week: + White Spot Syndrome Virus (WSSV) c2) week: + Vibrio harveyi (subcutaneous injection)

26 Feeding Peptides: More Hemolymph With Stress TP: Total hemolymph protein (g/dl) THC: Total hemocyte count (x 10 6 ) MR: Mortality rate (%)) Man value with different superscript are significantly different (p<0.05)

27 Feeding Peptides: More Hemolymph and Hemocytes; less Mortality With Stress TP: Total hemolymph protein (g/dl) THC: Total hemocyte count (x 10 6 ) MR: Mortality rate (%)) Man value with different superscript are significantly different (p<0.05)

28 Summary: Feeding peptides to shrimps UNDER STRESS, leads to Mortality reduction Immunity response stimulation Growth improvement

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