Annex to the Accreditation Certificate D-PL according to DIN EN ISO/IEC 17025:2005
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1 Deutsche Akkreditierungsstelle GmbH Annex to the Accreditation Certificate D-PL according to DIN EN ISO/IEC 17025:2005 Period of validity: to Date of issue: Holder of certificate: Federal State Budgetary Institution Bryansk Interregional Veterinary laboratory (FSBI "Bryanskaya IVL") Bryansky district, Bryansk region Shosseynaya Street 7, SUPONEVO, RUSSIAN FEDERATION Tests in the fields: physical, physico-chemical, chemical and microbiological tests of foodstuff, grain and products of its processing, animal feeding stuff, compound feeding stuff and raw material for animal feeding stuff Abbreviations used: see last page Within the given testing field marked with */**, the testing laboratory is permitted, without being required to inform and obtain prior approval from DAkkS, the following: *) the free choice of standard or equivalent testing methods. **) the modification, development and refinement of testing methods. The listed testing methods are exemplary. The testing laboratory maintains a current list of all testing methods within the flexible scope of accreditation. The testing laboratory is permitted, without being required to inform and obtain prior approval from DAkkS, to use standard testing methods listed here with different issue dates. 1/10
2 1 Physical, physico-chemical and chemical Analysis 1.1 Determination of Moisture in Milk, Milk Products, Grain and Animal Feeding Stuff using Thermogravimetry GOST GOST GOST R Grain Method of moisture content determination Dry canned milk Methods for determination of moisture Animal feeding stuffs. Determination of moisture content 1.2 Determination of Protein in Milk Products, Grain and Products of its Processing, Animal Feeding Stuff, Compound Feeding Stuff using Kjeldahl Method GOST GOST GOST R GOST R Grain and products of its processing Method for determination of protein Feeds, mixed feeds and raw material Determination of mass fraction of nitrogen and calculation of mass fraction of crude protein. Part 1: Kjeldahl method Feeds, mixed feeds and raw material Determination of mass fraction of nitrogen and calculation of mass fraction of crude protein, Kjeldahl method Milk and milk-containing products Determination of mass part of protein by Kjeldahl method 1.3 Determination of Fat in Grain and Products of its Processing, Animal Feeding Stuff, Compound Feeding Stuff and Raw Material for Animal Feeding Stuff using Soxhlet Method GOST GOST Feeds, mixed feeds, feed raw material. Methods for determining the raw fat content Grain and derived products. Determination of fat content Period of validity: to Date of issue: /10
3 GOST Feeds, mixed feeds and raw material. Method for determination of fat content 1.4 Determination of Ash in Animal Feeding Stuff, Compound Feeding Stuff and Raw Materials for Animal Feeding Stuff using Thermogravimetry GOST Fodder, mixed fodder and mixed fodder raw material. Methods for determination of raw ash 1.5 Determination of the Pesticides Residues in Vegetables and Fruits using Gas Chromatography and Mass Spectrometry (GC/MS) ** FR WI-M ver Measuring technique of determination of the pesticides residues in vegetables, fruits, cereals and soil by the method of Chromatography and Mass Spectrometry (Modification: only the following Parameters: Prometrin, Malathion, Penconazole, Chlorpyrifos ethyl, Triadimenol, Cyprodinil, Triadimefon, Iprodione, Bifenthrin, Phozalon, Lambdacyhalothrin, Cypermethrin, Esfenvalerate, Deltamethrin, Azoxystrobin, Diazinon, Fenvalerate, Chlorprophame, Propargite, Fluazifop-p-butyl, Procymidone, Fenitrothion, Parathionmethyl, Metribuzin, Diazinon) Determination of pesticides residues in vegetables and fruits technique: GC/MS (Modification: only the following Parameters: Azoxystrobin, Boscalid, Chlorpyrifos, Cyprodinil, Diazinon, Dimethoate, Fluazifop-p-butyl, Malathion, Metribuzin, Penconazole, Phozalone, Pirimicarb, Pirimiphos-methyl, Prometrin, Propargite, Pyraclostrobin, Pyrimethanil, Triadimenol, Triadimefon, Trifloxystrobin, Iprodione, Bifenthrin, Lambda- cyhalothrin, Esfenvalerate, Fenitrothion, Procymidone, Bromopropylate, Fenvalerate, Cypermethrin, Deltamethrin) Period of validity: to Date of issue: /10
4 1.6 Determination of Pesticides Residues in Vegetables and Fruits using Liquid Mass Spectrometry (LC/MS-MS) ** FR WI-M ver WI-M ver Measuring technique of determination of the pesticides residues in vegetables, fruits, cereals and soil by the method of Chromatography and Mass Spectrometry (Modification: only the following Parameters: Azoxystrobin, Boscalid, Chlorpyrifos, Cyprodinil, Diazinon, Dimethoate, Fluazifop-p-butyl, Malathion, Metribuzin, Penconazole, Phozalone, Pirimicarb, Pirimiphos-methyl, Prometrin, Propargite, Pyraclostrobin, Pyrimethanil, Triadimenol, Triadimefon, Trifloxystrobin) Determination of pesticides residues by LC/MS-MS (UltiMate maxis impact) (Modification: only the following Parameters: Azoxystrobin, Bscalid, Chlorpyrifos, Cyprodinil, Diazinon, Dimethoate, Fluazifopp-butyl, Malathion, Metribuzin, Penconazole, Phozalone, Pirimicarb, Pirimiphos-methyl, Prometrin, Propargite, Pyraclostrobin, Pyrimethanil, Triadimenol, Triadimefon, Trifloxystrobin) Measuring technique of determination of the pesticides residues in vegetables and fruits by the method of liquid chromatography and mass spectrometry (Modification: only the following Parameters: Cyproconazole, Diazinon, Difenoconazole, Dimethoate, Dimethomorph, Fenarimol, Fenazaquin, Fenoxaprop-p-ethyl, Flutriafol, Imazalil, Imidacloprid, Kresoxim-methyl, Mefenpyr-diethyl, Metalaxyl, Metribuzin, Metsulfuron-methyl, Pirimicarb, Pirimiphos-methyl, Prochloraz, Propargite, Propiconazole, Pyraclostrobin, Pyrimethanil, Pyriproxyfen, Spiroxamine, Tebuconazole, Thiabendazole, Thiophanate-methyl, Triasulfuron, Trifloxystrobin, Tribenuron-methyl) 1.7 Preparation of Samples GOST Foodstuffs. Determination of trace elements. Preparation of samples by pressure digestion Period of validity: to Date of issue: /10
5 GOST R Foodstuffs Determination of trace elements. Preparation of samples by pressure digestion (Modification of EN 13805:2002) 1.8 Determination of Additives and Residual of Toxic Metal in Foodstuff and Food Raw Materials using Atomic Absorption Spectrometry * GOST GOST R GOST R GOST R М М MUK Foodstuffs. Determination of trace elements. Determination of total arsenic and selenium by hydride generation atomic absorption spectrometry (HGAAS) after pressure digestion (Modification of EN 14627:2002) Raw material and foodstuffs Atomic absorption method for determination of arsenic Foodstuff - Determination of trace elements Determination of total arsenic and selenium by hydride generation atomic absorption spectrometry (HGAAS) after pressure digestion (Modification of EN 14627:2002) Foodstuffs and animal feeding stuffs. Determination of mercury by Zeeman atomic absorption spectrometry Measuring technique of mass fraction of mercury in foodstuffs and food raw materials, feeds and raw materials for it production by electrothermal atomic absorption spectrometry using mercury analyzer RA- 915 and attachment PIRO 915+ Measuring technique for determination of mass fraction of cadmium, arsenic, tin, mercury, lead, chromium in foodstuff and food raw materials, feeds, mixed feeds and feed raw material Measuring technique of mass fraction of lead and cadmium in food-stuffs and food raw materials by the method of electrothermal atomic absorption spectrometry Period of validity: to Date of issue: /10
6 1.9 Determination of Residues of Veterinary Pharmaceuticals/Drugs in Foodstuff and Food Raw Materials using High Performance Liquid Chromatography Mass Spectrometry (LC/MS-MS) ** GOST GOST GOST R GOST R GOST R GOST R WI-M ver WI-M ver Food products, food raw materials. Method of determination of the nitrofuran metabolites by high performance liquid chromatography - mass spectrometry (HPLC-MS) Food products, food raw materials. Method for determination of the quinolone content by high performance liquid chromatography with mass spectrometry detector Food products, food raw materials Method of determination of the antibiotic residues of tetracycline group by high performance liquid chromatography mass spectrometry (LC/MS-MS) Food products, food raw materials - Method for determination of the nitrofuran metabolites residual content by high performance liquid chromatography-mass spectrometry (LC/MS-MS) Food products, feeds, food raw materials. Method for determination of the coccidiostatics content by high performance liquid chromatography-mass spectrometry (HPLC- MS) Food products, food raw materials. Method of determination of the sulfonamides, nitroimidazoles, penicillins, amphenicols by high performance liquid chromatography - mass spectrometry (Modification: only the following Parameters: Chloramphenicol, Dimetridazole, Ronidazole, un Rnidazole, Metronidazole hydroxide, Metronidazole, Ornidazole, Tinidazole) Determination of ractopamine and clenbuterol in meat and meat products by LC/MS-MS (Matrix: meat and meat products; only the following Parameters: Ractopamine, Clenbuterol) Determination of antibiotic residues of tetracycline group in meat and meat products by LC/MS-MS (Modification of GOST , EN 14627:2002) Period of validity: to Date of issue: /10
7 WI-M ver WI-М ver WI-М ver WI-M ver WI-M ver WI-M ver MU А1/ Determination of antibiotic residues of tetracycline group in milk and milk products by LC/MS-MS (Modification of GOST , EN 14627:2002) Food products, food raw materials. Method of determination of the chloramphenicol by high performance liquid chromatography - mass spectrometry (Parameter: only the following Parameter: Chloramphenicol) Method of determination of the nitroimidazoles in meat and meat products by high performance liquid chromatography - mass spectrometry (Modification: only the following Parameters: Dimetridazole, Ronidazole, Un ronidazole, Metronidazole hydroxide, Metronidazole, Ornidazole, Tinidazole) Method of determination of the nitroimidazoles in milk and milk products by high performance liquid chromatography - mass spectrometry (Modification: only the following Parameters: Dimetridazole, Ronidazole, Un ronidazole, Metronidazole hydroxide, Metronidazole, Ornidazole, Tinidazole) Determination of the residual quinolone content in meat and meat products by high-performance liquid chromatography with mass spectrometric detection (modified MU /23) (Matrix: meat and meat products) Determination of the residual quinolone content in milk and milk products by high-performance liquid chromatography with mass spectrometric detection (Modification: MU /23) (Matrix: milk and milk products) Methodology for residue testing and determination of macrolides, lincosamides, pleuromutilins in livestock products by high performance liquid chromatography-mass spectrometry (HPLC-MS) for arbitration Period of validity: to Date of issue: /10
8 MUK 228/ Methodology used in arbitration testing and determination of beta-adrenostimulators in animal feed, physiological saline solution, organs and tissues of animals by high performance liquid chromatography and mass spectrometry (Matrix: meat, meat products; only the following Parameters: Ractopamine, Clenbuterol) 1.10 Determination of Organic Contaminants in Food using Liquid Chromatography with UV- Detection * GOST R ISO Cheese and processed cheese. Determination of natamycin content. Part 2: High performance liquid chromatographic method (identical to ISO :2007) 1.11 Detection of Vegetable in Milk Fat using Gas Chromatography Mass Spectrometry * GOST Milk and milk products. Detection of vegetable fat by gas chromatography with mass spectrometric detection 2 Microbiological Analysis 2.1 Determination and Detection of Bacteria in Foodstuff using Cultural Microbiological Methods * GOST GOST GOST GOST Foodstuff Methods for determination quantity of mesophilic aerobes and facultative anaerobes Microbiology of food and animal feeding stuffs. Methods for detection and enumeration of sulfite-reducing bacteria growing under anaerobic conditions (Modification of ISO 15213:2003) (here only detection) Milk and milk products. Methods for determination of Staphylococcus aureus (Equivalent to GOST R : ) Foodstuffs. Methods for detection and determination of Escherichia coli (here only detection) Period of validity: to Date of issue: /10
9 GOST GOST GOST GOST GOST GOST ISO GOST R GOST R GOST R Poultry meat, edible offal and poultry meat ready-to-cook. Method for detection of Salmonellae Food products. Methods for the detection of Salmonella spp. (Modification of ISO 6579:2002) Food products. Methods for detection and quantity determination of coagulase-positive staphylococci and Staphylococcus aureus (Modification of ISO :1999, ISO :1999, ISO :1999) Food products. Methods for detection and quantity determination of coliformes (Modification of ISO 4831:2006, ISO 4832:2006) (Modification: only the following Parameters: Escherichia coli, Klebsiella, Enterobacteria, Citrobacter, Serratia) Food products. Methods for detection of Listeria monocytogenes (identical to ISO :1996 and ISO : 1996/Amd.1:2004) Microbiology of foods and animal feeding stuffs. General requirements and guidance for microbiological examinations (identical to ISO 7218:2007) (here for the determination of total bacterial count) Poultry meat, edible offal and ready-to-cook poultry meat - Method for quantity determination of mesophilic aerobic and facultative-anaerobic microorganisms Food products. Methods for detection and determination of Listeria monocytogenes bacteria (harmonized with ISO : 1998) Food products. Method for the detection of Salmonella spp. (Modification of ISO 6579:2002) Period of validity: to Date of issue: /10
10 GOST R GOST R GOST R GOST R GOST R Food products. Methods for detection and quantity determination of coagulase-positive staphylococci and Staphylococcus aureus (Modification of ISO :1999, ISO :1999, ISO :1999) Food products. Methods for detection and quantity determination of coliformes (Modification of ISO 4831:2006, ISO 4832:2006) (Modification: only the following Parameters: Escherichia coli, Klebsiella, Enterobacteria, Citrobacter, Serratia) Milk and milk products. Methods of microbiological analysis Poultry meat, edible offal and ready to cook products. Methods for detection and quantity determination of coliform bacteria Poultry meat, edible offal and poultry meat ready-to-cook. Method for detection and determination of Staphylococcus aureus Abbreviations used: DIN EN FR GOST GOST R IEC ISO M MUK WI-M German Institute for Standardisation (Deutsches Institut für Normung e. V.) European Standard Measuring technique approved by the Federal Agency for Technical Regulation and Metrology (Rostekhregulirovaniye) and by the Federal Service for Veterinary and Phitosanitary Surveilance of Russian Federation (Rosselkhoznadzor) Interstate Standard approved by Interstate Council for Standardization, Metrology and Certification (ISC) National Standard of the Russian Federation approved by the Federal Agency for Technical Regulation and Metrology (Rostekhregulirovaniye) International Electrotechnical Commission International Organization for Standardization Methodical instruction specified by the manufacturer of the equipment and approved by the Federal Agency for Technical Regulation and Metrology (Rostekhregulirovaniye) Methodical instruction on inspection methods of Federal service in sphere of protection of costumers and human well-being of Ministry of Health and Social Development of the Russian Federation, approved by the head of Federal service in sphere of protection of costumers and human well-being. Laboratory in-house test method of Federal State Budgetary Institution Bryansk Interregional Veterinary laboratory, FSBI "Bryankaya IVL" Period of validity: to Date of issue: /10
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