Reproducible Separation of Carbohydrate, Oligosaccharide, and Organic Acid Analysis. Accurate and reproducible analysis Long column lifetimes

Transcription

1 Reproducible Separation of Carbohydrate, Oligosaccharide, and Organic Acid Analysis Accurate and reproducible analysis Long column lifetimes

2 Trust Rezex For Your HPLC Analysis Of Carbohydrates, Oligosaccharides, And Organic Acids Phenomenex Rezex HPLC ion-exclusion and ion-exchange columns are guaranteed to give you the performance you need. From drug formulation analysis to quality control testing of finished food products, Rezex columns consistently provide accurate and reproducible results. Try Rezex columns today risk-free and be rewarded with long lasting quality. Try Rezex Risk Free! Rezex is a guaranteed alternative to: Bio-Rad Aminex Waters Sugar-Pak Supelco Supelcogel Transgenomic CarboSep If you are not completely satisfied with the performance of any Rezex column, as compared to a competing product of the same size and phase, simply return the Rezex column with your comparative data within days for a FULL REFUND. 009 Phenomenex, Inc. All rights reserved.

3 For Excellent Resolution And Reproducibility Of Sugars, Starches, Organic Acids, And Oligonucleotides Broad Range of Phases to Perfectly Suit Your Application Needs... Column Selection - Variety Gives You the Power of Optimization... See the Difference! The Rezex Performance Advantage Sharper Peak Shape = Easy & Accurate Quantitation... Lower Backpressure = Longer Column Lifetimes & Faster Run Time Capability... Higher Efficiency = Baseline Separation of Critical Sample Components... The Proof is in the Performance Select Applications Food and Beverages... Fermentation / Bioethanol... Carbohydrates / Saccharides Alcohols...8 Other Applications...9 Specifications and Operating Recommendations...0 Ordering Information...

4 Broad Range Of Phases To Perfectly Suit Your Application Needs Rezex columns achieve separations based on multiple modes of interaction including ion-exchange, ion-exclusion, size exclusion, reversed phase, and partition. In many cases, this allows for separation of multiple compound classes with one column. The materials exhibit excellent strength and durability while maintaining low system backpressures not typical with highly cross-linked resins. The % cross-linked silver and sodium phases have a larger effective pore volume and are ideal for larger starches, such as oligosaccharides. Table Phenomenex Rezex Phase Available Description Applications Cross Referencing Guide Bio-Rad Supelco Waters Transgenomic Aminex Supelcogel Sugar-Pak RCM-Monosaccharide (L9 packing)* RHM-Monosaccharide (L7 packing)* RAM-Carbohydrate RSO-Oligosaccharide RNO-Oligosaccharide RPM-Monosaccharide (L packing)* RNM-Carbohydrate (L8 packing)* ROA-Organic Acid (L packing)* RFQ-Fast Acid RKP-Potassium RCU-USP Sugar Alcohols (L9 packing)* CALCIUM HYDROGEN SILVER % cross-linked SILVER % cross-linked SODIUM LEAD SODIUM HYDROGEN HYDROGEN POTASSIUM CALCIUM Monosaccharides and sugar alcohols, including sorbitol and mannitol from sweeteners and corn and cane sugars Class separation of di-, tri-, and tetra- saccharides Monosaccharides in combination with organic acids, fatty acids, alcohols, ketones, neutral compounds, or inorganic salts HPX-87C -009 HPX-87H -00 Supelcogel Ca Supelcogel C-0H & H Sugar-Pak CARBOSep CHO-80 ICSep ION-00 Unique selectivity complimentary to other Rezex column types High resolution of oligosaccharides up to 8 degrees of polymerization (Dp) HPX-A Supelcogel Ag & Ag High resolution of oligosaccharides CARBOSep COREGEL-87N 00 x 7.8 mm dimensions available for sugar alcohol analysis according to the USP procedure Monosaccharides and sugar alcohol analysis Cellobiose, glucose, xylose, arabinose, and mannose and other cellulose products Analysis of mono-, di-,and tri- saccharides For matrices which contain high concentration of inorganic sodium (i.e.-molasses) Organic acids Organic acids in combination with carbohydrates, alcohols, fatty acids, or neutral compounds Amino sugars Rapid screening of fruit quality Ethanol, acetic acid, glycerol, & standard alcohol mixtures HPX-87P HPX-87N -0 HPX-87H -00 Fast Acid -000 Analysis of glyphosate HPX-87K -0 Sugar analysis according to the USP procedures on a 0 x.0 mm dimension Sugar Alcohols -009 Supelcogel Pb CARBOSep COREGEL-87P Supelcogel C-0H & H Supelcogel K CARBOSep COREGEL-87K * United States Pharmacopeia (USP)

5 Column Selection Variety Gives You The Power Of Optimization Rezex columns utilize different ligands. Selecting the right column will allow you to achieve the best resolution for your peaks of interest. Column Selection Guideline:. Application examples (Table ). Cross referencing guide (Table ). Retention time chart (Table ). Our support staff Knowledgeable consultants who can provide excellent recommendations on applications Retention Times For Some Carbohydrates And Sugar Alcohols Still not sure what phase to choose? It is important to ensure that you get adequate resolution between your peaks of interest. The retention time chart below gives the retention times for common carbohydrates and sugar alcohols on the most popular Rezex phases. Table Counter Ion Analyte RAM Ag + RCM Ca + RNM Na + RHM H + RPM Pb + Adonitol (Ribitol) D-Altrose D-(-)-Arabinose D-(+)-Cellobiose D-(+)-Digitoxose Dulcitol Meso-Erythritol D-(-)- Fructose L-(-)-Fucose D-(+)-Galactose Gentiobiose D-(+)-Glucose Inositol Isomaltose Lactose Lactulose D- Lyxose D- Maltose Maltotriose Maltulose * Partial hydrolysis results. Counter Ion Analyte RAM Ag + RCM Ca + RNM Na + RHM H + RPM Pb + D- Mannitol D-(+)-Mannose Melibiose D-(+)-Melezitose * 9.9 D-(+)-Raffinose * 0.8 L-(+)-Rhamnose D-(-)-Ribose Salicin D-Sorbitol Stachyose Sucrose *.00 Trehalose Xylitol D-(+)-Xylose Conditions Dimensions: 00 x 7.8 mm (degassed) Temperature: 80 0 C

6 Phenomenex Rezex vs. Bio-Rad Aminex Sharper Peak Shape = Easy & Accurate Quantitation Saccharides Orange Juice Rezex ROA Superior Peak Shape App ID Rezex RCM Easier Quantitation App ID min min Aminex HPX-87C App ID Aminex HPX-87C App ID min Columns: Rezex RCM-Monosaccharide Aminex HPX-87C Dimensions: 00 x 7.8 mm Temperature: 80 C Sample:. Melezitose. Mannose. Maltose. Fructose. Glucose. Ribitol min Columns: Rezex RCM-Monosaccharide Aminex HPX-87C Dimensions: 00 x 7.8 mm Temperature: 80 C Sample:. Sucrose. Glucose. Fructose vs. sugar alcohols 98 & 99 Organic Acids in Wine Sugar Alcohols Rezex ROA Aminex HPX-87H 0 0 Columns: Rezex ROA-Organic Acid Aminex HPX-87H Dimensions: 00 x 7.8 mm Mobile Phase: 0.00 N H SO Flow Rate: 0. ml/min Detection: 0 nm Temperature: 0 C Sample:. Citric acid. Lactic acid. Tartaric acid. Acetic acid. Succinic acid Sharper Peaks App ID 97 min App ID 97 min Rezex RPM min Aminex HPX-87P No Peak Fronting Columns: Rezex RPM-Monosaccharide Aminex HPX-87P Dimensions: 00 x 7.8 mm Temperature: 80 C Sample:. Glucose. Xylitol. Mannitol. Erythritol. Sorbitol min App ID 98 App ID 99 Aminex and Bio-Rad are registered trademarks of Bio-Rad Laboratories, Inc. Comparative separations may not be representative of all applications.

7 Phenomenex Rezex vs. Bio-Rad Aminex Lower Backpressure = Longer Column Lifetimes and Faster Run Time Capability Aliphatic Acids Saccharides Rezex ROA Lower Backpressure min Aminex HPX-87H Rezex Aminex Bar Bar Columns: Rezex ROA-Organic Acid Aminex HPX-87H Dimensions: 00 x 7.8 mm Mobile Phase: 0.00 N H SO Flow Rate: 0. ml/min Detection: 0 nm Temperature: 0 C Sample:. Oxalic acid. Succinic acid. Citric acid. Formic acid. Tartaric acid. Acetic acid min App ID 9 App ID Rezex RPM Lower Backpressure Aminex HPX-87P Rezex 7 Aminex 7 Bar Bar Columns: Rezex RPM-Monosaccharide Aminex HPX-87P Dimensions: 00 x 7.8 mm Temperature: 7 C Sample:. Melezitose. Mannose. Maltose. Fructose. Glucose. Ribitol 0 0 min min App ID 98 App ID 98 vs. Higher Efficiency = Baseline Separation of Critical Sample Components Sugars 977 & 978 Sugars Oligosaccharides Rezex RCM Baseline Resolution App ID Rezex RSO Improved Resolution for Multiple Degrees of Polymerization Dp0 Dp Dp9 Dp8 Dp Dp7 Dp Dp Dp Dp Dp App ID min min Aminex HPX-87C App ID Aminex HPX-A Dp8 Dp9 Dp0 Dp Dp Dp Dp7 Dp Dp Dp Dp App ID min Columns: Rezex RCM-Monosaccharide Aminex HPX-87C Dimensions: 00 x 7.8 mm Temperature: 80 C Sample:. Sucrose. Fructose. Glucose. Sorbitol. Galactose min Columns: Rezex RSO-Oligosaccharide Aminex HPX-A Dimensions: 00 x 0.0 mm Flow Rate: 0. ml/min Temperature: 80 C Sample:. Malto-Oligosaccharides (Dp-Dp) (Dp refers to degree of polymerization) Aminex and Bio-Rad are registered trademarks of Bio-Rad Laboratories, Inc. Comparative separations may not be representative of all applications.

8 Select Applications On Rezex HPLC Columns Food and Beverage 970 Apple Juice White Wine 0 Honey App ID 0 App ID 970 App ID 0 0 min min min Column: Rezex RCM-Monosaccharide Dimensions: 00 x 7.8 mm Part No.: 00H-00-K0 Temperature: 7 C Sample:. Sucrose. Glucose. Fructose Column: Rezex ROA-Organic Acid Dimensions: 00 x 7.8 mm Part No.: 00H-08-K0 Mobile Phase: 0.00 N H SO Flow Rate: 0. ml/min Detection: 0 nm Temperature: 0 C Sample:. Citric acid. Tartaric acid. Succinic acid. Lactic acid. Acetic acid Column: Rezex RCM-Monosaccharide Dimensions: 00 x 7.8 mm Part No.: 00H-00-K0 Temperature: 7 C Sample:. Dp. Dp. Glucose. Fructose Fermentation and Bioethanol Fermentation Broth Bioethanol Bioethanol Great Resolution of Dp+ and Dp! Reduce run times by 0 % min 0 min 0 min App ID 987, 988, min 7 App ID min App ID 8 Column: Rezex RCM-Monosaccharide Dimensions: 00 x 7.8 mm Part No.: 00H-00-K0 Flow Rate: 0. ml/min Temperature: 80 C Sample:. Sucrose. Glucose. Galactose. Fructose Column: Rezex ROA-Organic Acid Dimensions: 00 x 7.8 mm Part No.: 00H-08-K0 Mobile Phase: 0.00 N Sulfuric 0 C Temperature: 0 C System: Shimadzu Prominence LC-0A System Sample:. Dp+. Lactic Acid. Dp. Glycerol. Maltose 7. Acetic Acid. Glucose 8. Ethanol Column: Rezex ROA-Organic Acid Dimensions: 0 x 7.8 mm Part No.: 00F-08-K0 Mobile Phase: 0.00 N Sulfuric 0 C Temperature: 0 C System: Shimadzu Prominence LC-0A System Sample:. Dp+. Lactic Acid. Dp. Glycerol. Maltose 7. Acetic Acid. Glucose 8. Ethanol

9 Select Applications On Rezex HPLC Columns Saccharides 99 Monosaccharides with Organic Acids Saccharides Saccharides App ID 99 App ID 0 App ID min 0 8 min min Column: Rezex RHM-Monosaccharide Dimensions: 00 x 7.8 mm Part No.: 00H-0-K0 Mobile Phase: 0. % formic acid Flow Rate: 0. ml/min Temperature: 7 C Sample:. Citric acid. Tartaric acid. Glucose. Fructose. Succinic acid Column: Rezex RCM-Monosaccharide Dimensions: 00 x 7.8 mm Part No.: 00H-00-K0 Temperature: 8 C Sample:. Melezitose. Maltose. Glucose. Mannose. Fructose. Ribitol Column: Rezex RAM-Carbohydrate Dimensions: 00 x 7.8 mm Part No.: 00H-0-K0 Temperature: 7 C Sample:. Melezitose. Mannose. Maltose. Fructose. Glucose Carbohydrates Carbohydrates Carbohydrates Carbohydrates 0 7 App ID 97 App ID App ID min 0 min 0 min Column: Rezex RAM-Carbohydrate Dimensions: 00 x 7.8 mm Part No.: 00H-0-K0 Temperature: 80 C Sample:. Sucrose. Glucose. Arabinose. Ribose Column: Rezex RCU-USP Sugar Alcohols Dimensions: 0 x.0 mm Part No.: 00G-00-D0 Flow Rate: 0. ml/min Detection: Rl Temperature: 0 C Sample:. Mannitol. Sorbitol Column: Rezex RNM-Carbohydrate Dimensions: 00 x 7.8 mm Part No.: 00H-0-K0 Temperature: 7 C Sample:. Stachyose. Cellobiose. Glucose. Fructose. Arabinose. Ribose 7. Salicin ( % solution of each) 7

10 Select Applications On Rezex HPLC Columns Carbohydrates / Saccharides (Cont d) 08 Saccharides Carbohydrates Wood Saccharides and Alcohols App ID 0 App ID 97 App ID min min min Column: Rezex RKP-Potassium Dimensions: 00 x 7.8 mm Part No.: 00H--K0 Flow Rate: 0. ml/min Temperature: 8 C Sample:. Maltotriose. Maltose. Glucose Column: Rezex RPM-Monosaccharide Dimensions: 00 x 7.8 mm Part No.: 00H-0-K0 Temperature: 8 C Sample:. D-Cellobiose. a-d-glucose. D-(+)-Xylose. D-(+)-Galactose. D-(-)-Arabinose Column: Rezex RPM-Monosaccharide Dimensions: 00 x 7.8 mm Part No.: 00H-0-K0 Temperature: 7 C Sample:. Stachyose 8. Mannitol. Maltose 9. Salicin. Glucose 0. Xylitol. Xylose. Sorbitol. Galactose. Fructose 7. meso-erythritol Alcohols Alcohols Carboxylic Acids Amino Sugars min App ID 0 8 min App ID min App ID Column: Rezex RHM-Monosaccharide Dimensions: 00 x 7.8 mm Part No.: 00H-0-K0 Detection: Rl Temperature: 0 C Sample:. Methanol. Ethanol. Isopropanol. n-propanol. sec-butanol. n-butanol Column: Rezex ROA-Organic Acid Dimensions: 00 x 7.8 mm Part No.: 00H-08-K % Trifluoroacetic Acid Flow Rate:.0 ml/min Temperature: 0 C Sample:. Acetylene Carboxylic Acid. Maleic Acid. Succinic Acid. Fumaric Acid Column: Rezex ROA-Organic Acid Dimensions: 00 x 7.8 mm Part No.: 00H-08-K0 Mobile Phase: % Phosphoric Acid Temperature: Ambient Sample:. Glucose. N-Acetylglucose. N-Acetylgalactosamine 8

11 Select Applications On Rezex HPLC Columns Other Applications Food Softeners Oligosaccharides Oligosaccharides Dp min App ID 8 Dp Dp Dp Dp Dp7 Dp8 Dp9 Dp0 Dp Dp Dp Dp Dp 0 min App ID min App ID 0 Column: Rezex RCM-Monosaccharide Dimensions: 00 x 7.8 mm Part No.: 00H-00-K0 Flow Rate: 0. ml/min Temperature: 0 C Sample:. Glycerol. Methoxypolyethylene Glycol. Triethylene Glycol. Sorbitol. Urea Column: Rezex RSO-Oligosaccharide Dimensions: 00 x 0 mm Part No.: 00P-0-N0 Flow Rate: 0. ml/min Detection: Rl Temperature: 7 C Sample: Malto-Oligosaccharides as shown Column: Rezex RPM-Monosaccharide Dimensions: 00 x 7.8 mm Part No.: 00D-0-K0 (Ambient) Column Temperature: 80 C Sample:. Mannitol. Sorbitol Applications (partial list) Compound Rezex Phase App ID No. Acetic acid ROA RHM 9 Acetylene carboxylic acid ROA 7 Arabinose RNM 0 RAM 97 Citric acid ROA RHM 970 D-Cellobiose RNM 0 RPM 97 D-(+)-Galactose RPM 97 D-(+)-Xylose RPM 97 D-(-)-Arabinose RPM 97 Erythritol RPM 98 Ethanol ROA Formic acid ROA RHM 9 Fructose RCM 0, 0, 97, 98, 977,987 RPM 08, 98 RNM 0 RAM 9 Fumaric acid ROA 7 Galactose RPM 08 RCM 977, 987 Glucose RCM 0, 0, 97, 977, 979, 98, 987, 98 RPM 08, 98 RKP 0 ROA RNM 0 RAM 9, 97 Glycerol RCM 8 Isopropanol ROA Lactic acid RHM 970, 97 Maleic acid ROA 7 Malto-Oligosaccharides RSo 07 Maltose RCM 0, 97 RPM 08, 98 RKP 0 RAM 9 Maltotriose RKP 0 Mannitol RPM 08, 98 RCU Compound Rezex Phase App ID No. Mannose RCM 0, 97 RPM 98 RAM 9 Melezitose RCM 0, 97 RPM 98 RAM 9 meso-erythritol RPM 08 Methanol ROA Methoxypolyethylene Glycol RCM 8 n-butanol ROA n-propanol ROA N-Acetylgalactosamine ROA N-Acetylglucose ROA Oxalic acid ROA RHM 9 Ribose RNM 0 RAM 97 Ribitol RCM 0, 97 RAM 9 RHM 99 RPM 98 Salicin RPM 08 RNM 0 sec-butanol ROA Sorbitol RPM 08, 98 RCM 8, 977 RCU Stachyose RPM 08 RNM 0 Succinic acid ROA RHM 9 Sucrose RCM 0, 98, 977, 987 RAM 97 Tartaric acid RCM 0 RHM 970 Triethylene glycol RCM 8 Urea RCM 8 Xylitol RPM 08, 98 Xylose RPM 08 * For applications not in this brochure, copies are available through Phenomenex or your local Phenomenex distributor. Call us for assistance in selecting the right column for your needs 9

12 Specifications And Operating Recommendations RCM Monosaccharide RSO Oligosaccharide RNO Oligosaccharide RNM Carbohydrate RAM Carbohydrate Part No. 00H-00-K0 00P-0-N0 00P-07-N0 00H-0-K0 00H-0-K0 Ionic Form Calcium Silver Sodium Sodium Silver Standard Dimensions 00 x 7.8 mm 00 x 0 mm 00 x 0 mm 00 x 7.8 mm 00 x 7.8 mm Matrix Sulfonated Styrene Divinyl Benzene Cross Linking 8 % % % 8 % 8 % Particle Size 8 µm µm µm 8 µm 8 µm Min. Efficiency (p/m) based on last peak,000 0,000,000 Typical Pressure Max Flow Rate) Max. Pressure Max Flow Rate), ,000,000 Max. Flow Rate (ml/min) 0. (see pressure) Max. Temperature ( C) Typical Mobile Phase Water Water Water Water Water ph Range Neutral Neutral Neutral Neutral Neutral Guard Column Part No. 0B-00-K0 0R-0-N0 0R-07-N0 0B-0-K0 0B-0-K0 Cleaning, Regeneration and Storage Organic Modifiers (Max) 0 % Methanol, IPA, EtOH, Acetonitrile Inorganic Modifiers % CaSO, Ca(NO ), CaCl % Silver Nitrate % Sodium Salts % Sodium Salts % Silver Nitrate Avoid >0 % Organic, > % Organic, >0 % Organic, >0 % Organic, > % Organic, Acids, Bases, Acids, Bases, Acids, Bases, Acids, Bases, Acids, Bases, Non-Calcium Salts/ Non-Silver Salts/ Non-Sodium Salts/ Non-Sodium Salts/ Non-Silver Salts/ Metal Ions Metal Ions Metal Ions Metal Ions Metal Ions Cleaning Solvent 00 % Water 00 % Water 00 % Water 00 % Water 00 % Water Flow Rate(mL/min) Temperature ( C) Duration (hrs) Regeneration Solvent 0. M Ca(NO ) 0. M AgNO 0. M NaNO 0. M NaNO 0. M AgNO Flow Rate (ml/min) Temperature ( C) Duration (hrs) Ship/Storage Solvent Water Water Water Water Water RPM Monosaccharide RHM Monosaccharide ROA Organic Acid RFQ Fast Acid RCU Sugar Alcohols Part No. 00H-0-K0 00H-0-K0 00H-08-K0 00D-0-K0 00G-00-D0 Ionic Form Lead Hydrogen Hydrogen Hydrogen Calcium Standard Dimensions 00 x 7.8 mm 00 x 7.8 mm 00 x 7.8 mm 00 x 7.8 mm 0 x.0 mm Matrix Sulfonated Styrene Divinyl Benzene Cross Linking 8 % 8 % 8 % 8 % 8 % Particle Size 8 µm 8 µm 8 µm 8 µm 8 µm Min. Efficiency (p/m) based on last peak,000,000 0,000 (Acetic Acid) 0,000,000 Typical Pressure Max Flow Rate) Max. Pressure Max Flow Rate),000,000,000,000,000 Max. Flow Rate (ml/min) Max. Temperature ( C) Typical Mobile Phase Water Water 0.00 N H SO 0.00 N H SO Water ph Range Neutral Neutral Guard Column Part No. 0B-0-K0 0B-0-K0 0B-08-K0 0B-0-K0 0A-00-D0 Cleaning, Regeneration and Storage Organic Modifiers (Max) 0 % Methanol, IPA, EtOH, Acetonitrite Inorganic Modifiers % Lead Nitrate % HNO, H PO % HNO, H PO % HNO, H PO % CaSO, Ca(NO ), CaCl Avoid >0 % Organic, >0 % Organic, >0 % Organic, >0 % Organic, >0 % Organic, Acids, Bases, Acids, Bases, Salts, Acids, Bases, Salts, Acids, Bases, Salts, Acids, Bases, Non-Lead Salts/ Metal Ions Metal Ions, ph > Metal Ions, ph > Non-Calcium Salts Metal Ions or Metal Ions Cleaning Solvent 00 % Water 00 % Water 00 % Water 00 % Water 00 % Water Flow Rate(mL/min) Temperature ( C) Duration (hrs) Regeneration Solvent 0. M Pb(NO ) 0.0 M H SO 0.0 M H SO 0.0 M H SO 0. M Ca(NO ) Flow Rate (ml/min) Temperature ( C) Duration (hrs) Ship/Storage Solvent Water Water 0.00 N H SO 0.00 N H SO Water 0

13 Ordering Information Rezex Columns Guards Description Part No. SecurityGuard Analytical Cartridges require universal holder Part No.: KJO-8 Cross Linkage Ionic Form Size (mm) Price Part No. Size (mm) Price SecurityGuard Cartridges (mm) x.0 /0pk RCM-Monosaccharide 00H-00-K0 8 % Calcium 00 x 7.8 0B-00-K0 0 x 7.8 AJ0-9 RHM-Monosaccharide 00H-0-K0 8 % Hydrogen 00 x 7.8 0B-0-K0 0 x 7.8 AJ0-90 RAM-Carbohydrate 00H-0-K0 8 % Silver 00 x 7.8 AJ0-9 RSO-Oligosaccharide 00P-0-N0 % Silver 00 x 0.0 0R-0-N0 0 x 0.0 RNO-Oligosaccharide 00P-07-N0 % Sodium 00 x 0.0 0R-07-N0 0 x 0.0 RPM-Monosaccharide (for USP procedure) 00H-0-K0 00D-0-K0 8 % 8 % Lead Lead 00 x x 7.8 0B-0-K0 0B-0-K0 0 x x 7.8 RNM-Carbohydrate 00H-0-K0 8 % Sodium 00 x 7.8 0B-0-K0 0 x 7.8 AJ0-9 AJ0-9 ROA-Organic Acid 00H-08-K0 8 % Hydrogen 00 x 7.8 0B-08-K0 0 x 7.8 AJ0-90 ROA-Organic Acid 00F-08-K0 8 % Hydrogen 0 x 7.8 0B-08-K0 0 x 7.8 AJ0-90 ROA-Organic Acid 00G-08-E0 8 % Hydrogen 0 x. AJ0-90 RKP-Potassium 00H--K0 8 % Potassium 00 x 7.8 RFQ-Fast Acid 00D-0-K0 8 % Hydrogen 00 x 7.8 0B-0-K0 0 x 7.8 AJ0-90 RCU-USP Sugar Alcohols 00G-00-D0 8 % Calcium 0 x.0 0A-00-D0 0 x.0 AJ0-9 for ID:.-8.0 mm Column Cross Reference Chart Phenomenex Rezex Bio-Rad Aminex Supelco Supelcogel Waters Sugar-Pak RCM-Monosaccharide HPX-87C Supelcogel Ca Sugar-Pak -009 RHM-Monosaccharide HPX-87H Supelcogel C-0H & H -00 RPM-Monosaccaride HPX-87P Supelcogel Pb RNM-Carbohydrate HPX-87N -0 RSO-Oligosaccharide HPX-A Supelcogel Ag & Ag ROA-Organic Acid HPX-87H Supelcogel C-0H & H -00 RFQ-Fast Acid Fast Acid -000 RKP-Potassium HPX-87K Supelcogel K -0 RCU-USP Sugar Alcohols Sugar Alcohols -009 If you are not completely satisfied with the performance of any Rezex column, as compared to a competing product of the same size and phase, simply return the Rezex column with your comparative data within days for a FULL REFUND.

14 Phenex Syringe Filters For Sample and Solvent Filtration Prior to Chromatography! Less system downtime More consistent, reproducible results Increased column lifetime -7 mm Diameter for 0 ml sample volumes -8 mm Diameter for 0 00 ml sample volumes Membrane Type/Size Part No. Unit Price Part No. Unit Price 0. µm Phenex-RC (Regenerated Cellulose) Phenex-PES (Polyethersulfone) Phenex-PTFE (Polytetrafluoroethylene) Phenex-NY (Nylon) 0.0 µm Phenex-RC (Regenerated Cellulose) Phenex-PES (Polyethersulfone) Phenex-PTFE (Polytetrafluoroethylene) Phenex-NY (Nylon).0 µm Phenex-GF, (Glass Fiber) AF /pk AF /pk AF /pk AF /pk AF-08-00/pk AF /pk AF /pk AF /pk AF /pk AF /pk AF /pk AF-07-00/pk AF /pk AF-07-00/pk AF /pk AF /pk AF /pk AF /pk AF /pk AF /pk AF /pk AF /pk AF /pk AF /pk AF /pk AF-07-00/pk AF /pk AF-07-00/pk AF /pk Prefiltration of heavily contaminated or highly viscous samples. When used in-series preceding a membrane filter, clogging of the membrane filter is prevented and sample clean up is optimized. Outlet connection is luer lock. AF0-8- AF /pk 00/pk If Phenex Syringe Filters do not perform as well or better than your current syringe filter product of similar membrane, diameter and pore size, send in your comparative data within days and keep the Phenex products for FREE! Syringe filters are non-sterile. Housing is made of medical-grade polypropylene (PP) and offer luer lock inlet/slip outlet connections, unless otherwise indicated.. 7 mm diameter.. Glass fiber filters are 8 mm diameter and made of borosilicate. They will remove 90 % of all particles >. µm.. Housing material is methacrylate butadiene styrene (MBS) polymerisate. Also known as Cyrolite.. mm diameter.. Hydrophobic membrane. Can be made hydrophilic by pre-wetting with IPA.. 8 mm diameter. 7. Additional dimensions and membrane types are available. Please contact your local Phenomenex technical consultant or distributor for availability or assistance. 8. Larger quantity purchases at significant savings are available.

15 You Need Constant Temperature, Use A ThermaSphere TS-0 Column Heater! BENEFITS Improves chromatographic reproducibility Improves ruggedness of separation Improves peak efficiency and analyte quantification Improves baseline and overall detector performance ESSENTIAL FOR Monosaccharides and sugar alcohol analysis Organic acid analysis Oligosaccharide analysis Rapid Fruit Sugar Analysis FEATURES Compact and lightweight unit Transparent compartment cover Micro-fan provides rapid thermal equilibration Specifications Column Size Accommodated: Fits up to one 0 cm length column, or cm column with guard column. Multiple inlet and outlet slots allow the shortest length of tubing to be used with any length column. ThermaSphere TS-0 Column Heater Part No. Description Price EH0-707 I ThermaSphere TS-0 HPLC Column Heater -90 C, I 9 to VAC, 0/0 Hz EH0-708 Stand for ThermaSphere TS-0 HPLC Column Heater Temperature Range: From to 90 C in 0. C increments. Temperature Stability: ±0. C Calibration two-point, electronic, factory set. Accuracy: 0. C over the entire range. Power: volt DC universal power supply takes voltage inputs from 9 VAC to VAC, 0/0 Hz. CE approved.. The ThermaSphere TS-0 is warranted for one year parts and labor. Each unit is individually calibrated and comes with a Certificate of Performance. No adjustment or re-calibration is ever necessary. CE approved, UL and CSA approved power supplies.. Please specify Line Cord if other than North America (Australia, Germany, Italy and U.K. are available) Over-temperature Alarm: Audible with automatic heater shutoff if column temperature exceeds 0 C of target temperature. Auto-Off Timer: Count down timer with audible alarm turns off heater, settable to 0 days in days, hours, minutes and seconds. Injection Counter: Trigger on external switch closure. If you are not completely satisfied with the performance of your new TS-0 column header, simply return the unit within 0 days of purchase for a FULL REFUND. Trademarks Phenex, Rezex, ThermaSphere, and SecurityGuard are trademarks of Phenomenex, Inc. Cyrolite is a trademark of CY/RO Industries. Aminex and Bio-Rad are registered trademarks of Bio-Rad Laboratories, Inc. Waters is a registered trademark and Sugar-Pak is a trademark of Waters Corporation. Supelco is a registered trademark and SUPELCOGEL is a trademark of Sigma-Aldrich Biotechnology LP. Transgenomic is a registered trademark and CARBOSep is a trademark of Transgenomic, Inc. Disclaimer Comparative separations may not be representative of all applications. Subject to Phenomenex Standard Terms & Conditions, which may be viewed at Phenomenex, Inc. All rights reserved.

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