Carbohydrate. Organic Acid Analysis Oligosaccharide. Guaranteed alternative to: Bio-Rad Aminex Waters Sugar-Pak Supelco Supelcogel

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1 Organic Acid Analysis Oligosaccharide Carbohydrate Guaranteed alternative to: Bio-Rad Waters Sugar-Pak Supelco Supelcogel

2 HPLC Columns for Carbohydrate, Oligosaccharide, and Organic Acid Analysis on-exclusion and ion-exchange materials with a small degree of reversed phase selectivity % and sulfonated styrene-divinylbenzene (SDVB) Calcium (Ca + ), Sodium (Na + ), Hydrogen (H + ), Potassium (K + ), Lead (Pb + ), and Silver (Ag + ) ionic forms for wide spectrum of selectivities Analyze samples from foods, beverages, fermentation broths, and other complex matrices USP* L9, L7, L, L, and L packings available Phases to Perfectly Suit Your Application Needs f you are not completely satisfied with the performance of any Rezex column, as compared to a competing product of the same size and phase, simply return the Rezex column with your comparative data within days for a FULL REFUND. Phase Available Description Applications Bio-Rad Supelco Supelcogel Waters Sugar-Pak RCM-Monosaccharide (L9 packing)* RHM-Monosaccharide (L7 packing)* RAM-Carbohydrate RSO-Oligosaccharide RNO-Oligosaccharide RPM-Monosaccharide (L packing)* RNM-Carbohydrate (L packing)* ROA-Organic Acid (L packing)* RFQ-Fast Acid RKP-Potassium RCU-USP Sugar Alcohols (L9 packing)* * United States Pharmacopeia CALCUM HYDROGEN SLVER % cross-linked SLVER % cross-linked SODUM LEAD SODUM HYDROGEN HYDROGEN POTASSUM CALCUM Monosaccharides and sugar alcohols, including sorbitol and mannitol from sweeteners and corn and cane sugars Class separation of di-, tri-, and tetra- saccharides Monosaccharides in combination with organic acids, fatty acids, alcohols, ketones, neutral compounds, or inorganic salts HPX-87C -9 HPX-87H - Supelcogel Ca Sugar-Pak Supelcogel C-H & H Unique selectivity complimentary to other Rezex column types High resolution of oligosaccharides up to 8 degrees of polymerization (dp) HPX-A -97 Supelcogel Ag & Ag High resolution of oligosaccharides x 7.8mm dimensions available for sugar alcohol analysis according to the USP procedure Monosaccharides and sugar alcohol analysis. Cellobiose, glucose, xylose, arabinose, and mannose and other cellulose products Analysis of mono-, di-,and tri- saccharides For matrices which contain high concentration of inorganic sodium (i.e.-molasses) Organic acids Organic acids in combination with carbohydrates, alcohols, fatty acids, or neutral compounds Amino sugars Rapid screening of fruit quality Ethanol, acetic acid, glycerol, & standard alcohol mixtures HPX-87P -98 HPX-87N - HPX-87H - Fast Acid - Analysis of glyphosate HPX-87K - Sugar analysis according to the USP procedures on a x.mm dimension Sugar Alcohols -9 Supelcogel Pb Supelcogel C-H & H Supelcogel K

3 HPLC Columns for Carbohydrate, Oligosaccharide, and Organic Acid Analysis VARETY GVES YOU THE POWER OF OPTMZATON Compounds of interest can change from application to application. Shouldn t you have choices for an optimal separation? The example below illustrates that you can achieve varying results to suit your specific needs by selecting the appropriate phase. RSO APP D 99 min RHM APP D 99 8 min RPM APP D 98 min RCM APP D 97 min RAM APP D 9 min Saccharides Columns: Rezex RSO-Oligosaccharide Rezex RHM-Monosaccharide Rezex RPM-Monosaccharide Rezex RCM-Monosaccharide Rezex RAM-Carbohydrate Dimensions: x.mm (RSO Only) x 7.8mm Order No.: P--N H--K H--K H--K H--K Temperature: C Sample:. Melezitose. Maltose. Glucose. Mannose. Fructose. Ribitol RETENTON TMES FOR SOME CARBOHYDRATES AND SUGAR ALCOHOLS Still not sure what phase to choose? t is important to ensure that you get adequate resolution between your peaks of interest. The retention time chart below gives the retention times for common carbohydrates and sugar alcohols on the most popular Rezex phases. Counter on Analyte RAM Ag + RCM Ca + RNM Na + RHM H + RPM Pb + Adonitol (Ribitol) D-Altrose D-(-)-Arabinose D-(+)-Cellobiose D-(+)-Digitoxose Dulcitol Meso-Erythritol D-(-)- Fructose L-(-)-Fucose D-(+)-Galactose Gentiobiose D-(+)-Glucose nositol somaltose Lactose Lactulose D- Lyxose D- Maltose Maltotriose Maltulose * Partial hydrolysis results. Counter on Analyte RAM Ag + RCM Ca + RNM Na + RHM H + RPM Pb + D- Mannitol D-(+)-Mannose Melibiose D-(+)-Melezitose * 9.9 D-(+)-Raffinose *.8 L-(+)-Rhamnose D-(-)-Ribose Salicin D-Sorbitol Stachyose Sucrose *. Trehalose Xylitol D-(+)-Xylose Conditions Dimensions: x 7.8mm (degassed) Temperature: C

4 PHENOMENEX Rezex vs. BO-RAD See The Difference! EASER, ACCURATE QUANTTATON Due to improved peak shape Saccharides Orange Juice Rezex RCM APP D 97 Superior Peak Shape Rezex RCM APP D 98 Easier Quantitation min 8 min HPX-87C APP D 97 HPX-87C APP D 98 min Columns: Rezex RCM-Monosaccharide HPX-87C Dimensions: x 7.8mm Order No.: H--K Temperature: C Sample:. Melezitose. Mannose. Maltose. Fructose. Glucose. Ribitol 8 min Columns: Rezex RCM-Monosaccharide HPX-87C Dimensions: x 7.8mm Order No.: H--K Temperature: C Sample:. Sucrose. Glucose. Fructose APP D 97 vs. sugar alcohols 98 & 99 Organic Acids in Wine Sugar Alcohols Rezex ROA HPX-87H APP D 97 Sharper Peaks min min Rezex RPM APP D 98 min HPX-87P APP D 99 No Peak Fronting min Columns: Rezex ROA-Organic Acid HPX-87H Dimensions: x 7.8mm Order No.: H-8-K Mobile Phase:.N H SO Flow Rate:. ml/min Detection: nm Temperature: C Sample:. Citric acid. Lactic acid. Tartaric acid. Acetic acid. Succinic acid Columns: Rezex RPM-Monosaccharide HPX-87P Dimensions: x 7.8mm Order No.: H--K Temperature: C Sample:. Glucose. Xylitol. Mannitol. Erythritol. Sorbitol

5 PHENOMENEX Rezex vs. BO-RAD See The Difference! LONGER COLUMN LFETME AND FASTER RUN TMES Due to lower backpressures Aliphatic Acids Saccharides Rezex RHM APP D 9 min Rezex RPM APP D 98 min HPX-87H APP D 9 min Columns: Rezex RHM-Monosaccharide HPX-87H Dimensions: x 7.8mm Order No.: H--K Mobile Phase:.N H SO Flow Rate:. ml/min Detection: nm Temperature: C Sample:. Oxalic acid. Succinic acid. Citric acid. Formic acid. Tartaric acid. Acetic acid Lower Backpressure R e z e x Bars Bars HPX-87P APP D 98 min Columns: Rezex RPM-Monosaccharide HPX-87P Dimensions: x 7.8mm Order No.: H--K Temperature: 7 C Sample:. Melezitose. Mannose. Maltose. Fructose. Glucose. Ribitol Lower Backpressure R e z e x 7 7 Bars Bars vs. BASELNE SEPARATON OF CRTCAL SAMPLE COMPONENTS Sugars Due to improved resolution 977 & 978 Sugars Oligosaccharides Rezex RCM APP D 977 Baseline Resolution Rezex RSO APP D 9 mproved Resolution for Multiple Degrees of Polymerization Dp8 Dp9 Dp Dp Dp Dp7 Dp Dp Dp Dp Dp min min HPX-87C APP D 978 HPX-A APP D 9 Dp8 Dp9 Dp Dp Dp Dp Dp7 Dp Dp Dp Dp min Columns: Rezex RCM-Monosaccharide HPX-87C Dimensions: x 7.8mm Order No.: H--K Temperature: C Sample:. Sucrose. Fructose. Glucose. Sorbitol. Galactose min Columns: Rezex RSO-Oligosaccharide HPX-A Dimensions: x.mm Order No.: P--N Flow Rate:. ml/min Temperature: C Sample:. Malto-Oligosaccharides (Dp-Dp) (Dp refers to degree of polymerization)

6 Applications on Rezex HPLC Columns RCM-MONOSACCHARDE Ca + Apple Juice Saccharides Fermentation Broth min 8 min min min min APP D Column: Rezex RCM-Monosaccharide Dimensions: x 7.8mm Order No.: H--K Temperature: 7 C Sample:. Sucrose. Glucose. Fructose APP D Column: Rezex RCM-Monosaccharide Dimensions: x 7.8mm Order No.: H--K Temperature: 8 C Sample:. Melezitose. Maltose. Glucose. Mannose. Fructose. Ribitol APP D 987 APP D 988 APP D 989 Column: Rezex RCM-Monosaccharide Dimensions: x 7.8mm Order No.: H--K Flow Rate:. ml/min Temperature: C Sample:. Sucrose. Glucose. Galactose. Fructose 99 RHM-MONOSACCHARDE H + Monosaccharides with Organic Acids 97 White Wine 97 RAM-CARBOHYDRATE Ag + Carbohydrates 8 min APP D 99 Column: Rezex RHM-Monosaccharide Dimensions: x 7.8mm Order No.: H--K Mobile Phase:.% formic acid Flow Rate:. ml/min Temperature: 7 C Sample:. Citric acid. Tartaric acid. Glucose. Fructose. Succinic acid min APP D 97 Column: Rezex RHM-Monosaccharide Dimensions: x 7.8mm Order No.: H--K Mobile Phase:.N H SO Flow Rate:. ml/min Detection: nm Temperature: C Sample:. Citric acid. Tartaric acid. Succinic acid. Lactic acid. Acetic acid APP D 97 min Column: Rezex RAM-Carbohydrate Dimensions: x 7.8mm Order No.: H--K Temperature: C Sample:. Sucrose. Glucose. Arabinose. Ribose

7 Applications on Rezex HPLC Columns RCU-USP SUGAR ALCOHOL Ca + Honey Food Softeners Carbohydrates APP D min Column: Rezex RCM-Monosaccharide Dimensions: x 7.8mm Order No.: H--K Temperature: 7 C Sample:. Dp. Dp. Glucose. Fructose APP D 8 min Column: Rezex RCM-Monosaccharide Dimensions: x 7.8mm Order No.: H--K Flow Rate:. ml/min Temperature: C Sample:. Glycerol. Methoxypolyethylene Glycol. Triethylene Glycol. Sorbitol. Urea APP D min Column: Rezex RCU-USP Sugar Alcohols Dimensions: x.mm Order No.: G--D Flow Rate:. ml/min Detection: Rl Temperature: C Sample:. Mannitol. Sorbitol Saccharides RPM-MONOSACCHARDE Pb + 97 Wood Saccharides 8 Saccharides and Alcohols min min min APP D 9 Column: Rezex RAM-Carbohydrate Dimensions: x 7.8mm Order No.: H--K Temperature: 7 C Sample:. Melezitose. Mannose. Maltose. Fructose. Glucose APP D 97 Column: Rezex RPM-Monosaccharide Dimensions: x 7.8mm Order No.: H--K Temperature: 8 C Sample:. D-Cellobiose. α-d-glucose. D-(+)-Xylose. D-(+)-Galactose. D-(-)-Arabinose APP D 8 Column: Rezex RPM-Monosaccharide Dimensions: x 7.8mm Order No.: H--K Temperature: 7 C Sample:. Stachyose 8. Mannitol. Maltose 9. Salicin. Glucose. Xylitol. Xylose. Sorbitol. Galactose. Fructose 7. meso-erythritol

8 Applications on Rezex HPLC Columns ROA-ORGANC ACD H + Alcohols Carboxylic Acids Amino Sugars APP D min Column: Rezex ROA-Organic Acid Dimensions: x 7.8mm Order No.: H-8-K Detection: Rl Temperature: C Sample:. Methanol. Ethanol. sopropanol. n-propanol. sec-butanol. n-butanol APP D 7 8 min Column: Rezex ROA-Organic Acid Dimensions: x 7.8mm Order No.: H-8-K +.% Trifluoroacetic Acid Flow Rate:. ml/min Temperature: C Sample:. Acetylene Carboxylic Acid. Maleic Acid. Succinic Acid. Fumaric Acid APP D min Column: Rezex ROA-Organic Acid Dimensions: x 7.8mm Order No.: H-8-K Mobile Phase: % Phosphoric Acid Temperature: Ambient Sample:. Glucose. N-Acetylglucose. N-Acetylgalactosamine RKP-POTASSUM K + RNM-CARBOHYDRATE Na + RSO-OLGOSACCHARDE Ag + Carbohydrates Carbohydrates Oligosaccharides Dp 7 Dp Dp Dp Dp Dp7 Dp8 Dp9 Dp Dp Dp Dp Dp Dp min min min APP D Column: Rezex RKP-Potassium Dimensions: x 7.8mm Order No.: H--K Flow Rate:. ml/min Temperature: 8 C Sample:. Maltotriose. Maltose. Glucose APP D Column: Rezex RNM-Carbohydrate Dimensions: x 7.8mm Order No.: H--K Temperature: 7 C Sample:. Stachyose. Cellobiose. Glucose. Fructose. Arabinose. Ribose 7. Salicin (% solution of each) APP D 7 Column: Rezex RSO-Oligosaccharide Dimensions: x mm Order No.: P--N Flow Rate:. ml/min Detection: Rl Temperature: 7 C Sample: Malto-Oligosaccharides as shown 7

9 Applications on Rezex HPLC Columns This is only a partial list of applications. Compound Rezex phase App D No. Acetic acid ROA RHM 9 Acetylene carboxylic acid ROA 7 Arabinose RNM RAM 97 Citric acid ROA RHM 97 D-Cellobiose RNM RPM 97 D-(+)-Galactose RPM 97 D-(+)-Xylose RPM 97 D-(-)-Arabinose RPM 97 Erythritol RPM 98 Ethanol ROA Formic acid ROA RHM 9 Fructose RCM,, 97, 98, 977,987 RPM 8, 98 RNM RAM 9 Fumaric acid ROA 7 Galactose RPM 8 RCM 977, 987 Glucose RCM,, 97, 977, 979, 98, 987, 98 RPM 8, 98 RKP ROA RNM RAM 9, 97 Glycerol RCM 8 sopropanol ROA Lactic acid RHM 97, 97 Maleic acid ROA 7 Malto-Oligosaccharides RSO 7 Maltose RCM, 97 RPM 8, 98 RKP RAM 9 Maltotriose RKP Mannitol RPM 8, 98 RCU ORDERNG NFORMATON Compound Rezex phase App D No. Mannose RCM, 97 RPM 98 RAM 9 Melezitose RCM, 97 RPM 98 RAM 9 meso-erythritol RPM 8 Methanol ROA Methoxypolyethylene Glycol RCM 8 n-butanol ROA n-propanol ROA N-Acetylgalactosamine ROA N-Acetylglucose ROA Oxalic acid ROA RHM 9 Ribose RNM RAM 97 Ribitol RCM, 97 RAM 9 RHM 99 RPM 98 Salicin RPM 8 RNM sec-butanol ROA Sorbitol RPM 8, 98 RCM 8, 977 RCU Stachyose RPM 8 RNM Succinic acid ROA RHM 9 Sucrose RCM, 98, 977, 987 RAM 97 Tartaric acid RCM RHM 97 Triethylene glycol RCM 8 Urea RCM 8 Xylitol RPM 8, 98 Xylose RPM 8 * For applications not in this brochure, copies are available through or your local distributor. f you are not completely satisfied with the performance of any Rezex column, as compared to a competing product of the same size and phase, simply return the Rezex column with your comparative data within days for a FULL REFUND. SecurityGuard Cartridges require universal holder Order No.: KJO-8 REZEX Columns Guards SecurityGuard Cartridges Cross onic x.mm Description Order No. Linkage Form Size (mm) Price Order No. Size (mm) Price /8pk RCM-Monosaccharide H--K 8% Calcium x 7.8 B--K x 7.8 AJ-9 RHM-Monosaccharide H--K 8% Hydrogen x 7.8 B--K x 7.8 AJ-9 RAM-Carbohydrate H--K 8% Silver x 7.8 AJ-9 RSO-Oligosaccharide P--N % Silver x. R--N x. AJ-9 RNO-Oligosaccharide P-7-N % Sodium x. R-7-N x. RPM-Monosaccharide H--K 8% Lead x 7.8 B--K x 7.8 AJ-9 (for USP procedure) D--K 8% Lead x 7.8 B--K x 7.8 AJ-9 RNM-Carbohydrate H--K 8% Sodium x 7.8 B--K x 7.8 ROA-Organic Acid H-8-K 8% Hydrogen x 7.8 B-8-K x 7.8 AJ-9 RKP-Potassium H--K 8% Potassium x 7.8 RFQ-Fast Acid D--K 8% Hydrogen x 7.8 B--K x 7.8 AJ-9 RCU-USP Sugar Alcohols Column G--D 8% Calcium x. A--D x. AJ-9 Copyright, nc. Rezex and ThermaSphere are trademarks of. is a registered trademark of Bio-Rad. Sugar-Pak is a trademark of for D:.-8.mm Waters Corp. Supelcogel is a trademark of Supelco. is a registered trademark. Columns used for comparison were purchased directly from original column manufacturer. Comparisons may not be representative of every application. nformation, descriptions, and specifications in this publication are subject to change without notice. 8

10 Specifications and Operating Recommendations SPECFCATONS AND OPERATNG RECOMMENDATONS RCM Monosaccharide RSO Oligosaccharide RNO Oligosaccharide RNM Carbohydrate RAM Carbohydrate Order No. H--K P--N P-7-N H--K H--K onic Form Calcium Silver Sodium Sodium Silver Standard Dimensions x 7.8mm x mm x mm x 7.8mm x 7.8mm Matrix Sulfonated Styrene Divinyl Benzene Cross Linking 8% % % 8% 8% Particle Size 8µ µ µ 8µ 8µ Min. Efficiency (p/m) based on last peak,,, Typical Pressure Max Flow Rate) Max. Pressure Max Flow Rate) Max. Flow Rate (ml/min). (see pressure).... Max. Temperature ( C) Typical Mobile Phase Water Water Water Water Water ph Range Neutral Neutral Neutral Neutral Neutral Guard Column Order No. B--K R--N R-7-N B--K B--K Cleaning, Regeneration and Storage Organic Modifiers (Max) % Methanol, PA, EtOH, Acetonitrile norganic Modifiers % CaSO, Ca(NO ), CaCl % Silver Nitrate % Sodium Salts % Sodium Salts % Silver Nitrate Avoid >% Methanol, >% Methanol, >% Methanol, >% Methanol, >% Methanol, Acids, Bases, Acids, Bases, Acids, Bases, Acids, Bases, Acids, Bases, Non-Calcium Salts/ Non-Silver Salts/ Non-Sodium Salts/ Non-Sodium Salts/ Non-Silver Salts/ Metal ons Metal ons Metal ons Metal ons Metal ons Cleaning Solvent % Water % Water % Water % Water % Water Flow Rate(mL/min)..... Temperature ( C) Duration (hrs) Regeneration Solvent.M Ca(NO ).M AgNO.M NaNO.M NaNO.M AgNO Flow Rate (ml/min)..... Temperature ( C) Duration (hrs) Ship/Storage Solvent Water Water Water Water Water RPM Monosaccharide RHM Monosaccharide ROA Organic Acid RFQ Fast Acid RCU Sugar Alcohols Order No. H--K H--K H-8-K D--K G--D onic Form Lead Hydrogen Hydrogen Hydrogen Calcium Standard Dimensions x 7.8mm x 7.8mm x 7.8mm x 7.8mm x.mm Matrix Sulfonated Styrene Divinyl Benzene Cross Linking 8% 8% 8% 8% 8% Particle Size 8µ 8µ 8µ 8µ 8µ Min. Efficiency (p/m) based on last peak,,, (Acetic Acid),, Typical Pressure Max Flow Rate) Max. Pressure Max Flow Rate) Max. Flow Rate (ml/min)..... Max. Temperature ( C) Typical Mobile Phase Water Water.N H SO.N H SO Water ph Range Neutral Neutral - - Neutral Guard Column Order No. B--K B--K B-8-K B--K A--D Cleaning, Regeneration and Storage Organic Modifiers (Max) Up to % Methanol, PA, EtOH, Acetonitrite norganic Modifiers % Lead Nitrate % HNO, H PO % HNO, H PO % HNO, H PO % CaSO, Ca(NO ), CaCl Avoid >% Methanol, >% Methanol, >% Methanol, >% Methanol, >% Methanol, Acids, Bases, Acids, Bases, Salts, Acids, Bases, Salts, Acids, Bases, Salts, Acids, Bases, Non-Lead Salts/ Metal ons Metal ons, ph > Metal ons, ph > Non-Calcium Salts Metal ons or Metal ons Cleaning Solvent % Water % Water % Water % Water % Water Flow Rate(mL/min)..... Temperature ( C) 8 8 Ambient 8 8 Duration (hrs) Regeneration Solvent.M Pb(NO ).M H SO.M H SO.M H SO.M Ca(NO ) Flow Rate (ml/min)..... Temperature ( C) 8 8 Ambient 8 8 Duration (hrs) Ship/Storage Solvent Water Water.N HSO.N HSO Water 9

11 To mprove Your Carbohydrate and Organic Acid Reproducibility % OFF* (Any Single) TS- with your next Rezex column order You Need Constant Temperature, Use A ThermaSphere TS- Column Heater! BENEFTS mproves chromatographic reproducibility mproves ruggedness of separation mproves peak efficiency and analyte quantification mproves baseline and overall detector performance ESSENTAL FOR Monosaccharides and sugar alcohol analysis Organic acid analysis Oligosaccharide analysis Rapid Fruit Sugar Analysis FEATURES Compact and lightweight unit Transparent compartment cover Micro-fan provides rapid thermal equilibration Specifications Column Size Accommodated: Fits up to one cm length column, or cm column with guard column. Multiple inlet and outlet slots allow the shortest length of tubing to be used with any length column. Temperature Range: From to C in. C increments. Temperature Stability: ±. C Calibration two-point, electronic, factory set. Accuracy:. C over the entire range. Power: volt DC universal power supply takes voltage inputs from 9 VAC to VAC, / Hz. CE approved. Over-temperature Alarm: Audible with automatic heater shutoff if column temperature exceeds C of target temperature. Auto-Off Timer: Count down timer with audible alarm turns off heater, settable to days in days, hours, minutes and seconds. njection Counter: Trigger on external switch closure. ThermaSphere TS- Column Heater Order No. Description Price EH-77 ThermaSphere TS- HPLC Column Heater - C, 9 to VAC, / Hz EH-78 Stand for ThermaSphere TS- HPLC Column Heater. The ThermaSphere TS- is warranted for one year parts and labor. Each unit is individually calibrated and comes with a Certificate of Performance. No adjustment or re-calibration is ever necessary. CE approved, UL and CSA approval pending.. Please specify Line Cord if other than U.S. (Australia, Germany, taly and U.K. are available) * Offer cannot be combined with any other discount or promotional item. Discount valid only at time of purchase and must be specified at time of order. f you are not completely satisfied with the performance of your new TS- column heater, simply return the unit within days of purchase for A FULL REFUND.

12 PRNTED MATTER 9_