Certification GMP, HACCP Certification ISO 9001:2008

Transcription

1 LEADER IN BIOTECHNOLOGY PROBIOTIC SOYA FERMENTATION Certification GMP, HACCP Certification ISO 9001:2008 Evershining Ingredient Co.,Ltd. Thailand.

2 Soybean meal -- the cheapest protein supply ingredient. 1> High protein digestibility. 2> Good palatability. 3> Excellent amino acid balance.

3 Anti-nutritional factors: 1> Trypsin inhibitor. 2> Antigens: Glycinine. β-conglycinine. 3> Lectins. 4> NSP and Oligosaccharides. 5> Urease, goitrogens, saponins etc. Limitation of usage of SBM

4 Trypsin inhibitor Inactivates trypsin and chymotrypsin. Decreases the protein digestion. Non-digested protein will ferment in the colon. Increases the requirement for methionine & cystine.

5 Content of trypsin inhibitor in soy protein products PRODUCT MG PER GRAM PROTEIN RAW SOY BEAN UNTOASTED SOY BEAN MEAL TOASTED SOY BEAN MEAL SOY PROTEIN CONCENTRATE HP NattoGold 500 < 2

6 Antigens: Glycinine β-conglycinine Antigen Pathogen Toxin antigen v.s. small intestinal epithelium nutrients

7 Lectin (Hemagglutinin) Damages the intestinal epithelium of the villi. Inactivates the intestinal epithelium enzyme. Reduces absorption of nutrients. Increases endogenic protein loss.

8 Content of antigens in soy protein products PRODUCT β-conglycinine GLYCININE LECTIN UNTOASTED SOY BEAN MEAL HIGH HIGH O PPM TOASTED SOY BEAN MEAL HIGH HIGH 20,000 40,000 PPM PPM SOY PROTEIN CONCENTRATE < 10 PPM < 100 PPM < 1 PPM HP-300 < 10 PPM < 100 PPM < 1 PPM ( 2 PPM)??? < 1 PPM NattoGold 500 < 10 PPM < 100 PPM < 1 PPM

9 Total Ca arbohydrates NSP (%, AS IS) Pectins 11.0 Soybean Rice Wheat Corn meal bran bran Beta-glucans 1.8 Arabinoxylans Cellulose Oligo-saccharides Starch Lignin FINNFEEDS

10 Non Starch Polysaccharides (NSP) and Oligosaccharides In soybean meal the NSP and oligosaccharides mainly are Pectins, Cellulose and α- galactosidose. Monogastric animals are unable to digest NSP and Oligosaccharides. Increase the water holding capacity of the feed.(osmotic diarrhea and reduced feed intake) Increase the viscosity of the intestinal contents.(reduce digestibility) Degraded by gas-producing bacteria in the appendix and colon.(bloating)

11 Content of Oligosaccharides in soy protein products PRODUCT OLIGOSACCHARIDES % ph UNTOASTED SOY BEAN MEAL TOASTED SOY BEAN MEAL SOY PROTEIN CONCENTRATE HP NattoGold

12 α-galactosidose CH2 CH2 CH2 CH2 CH2OH O OH OH OH OH O O O O OH O OH O OH O OH O CH2OH OH OH OH OH OH OH Galactose Galactose Galactose Glucose Fructose Verbascose Stachyose Raffinose Sucrose

13 Lactose Sucrose CH2 CH2 CH2 CH2 CH2OH O OH OH OH OH O O O O OH O OH O OH O OH O CH2OH OH OH OH OH OH OH Galactose Galactose Galactose Glucose Fructose Lactobacillus Lactobacillus Lactobacillus Lactic acid Lactic acid Lactic acid

14 Oligosaccharides are converted to lactic acid, NattoGold 500 has 4.5% L-Lactic acid. Lactic acid is the best acidifier: Improved feed utilization,performance. Enhanced pepsin production. Inhibition of harmful microorganisms. Increase calcium utility of feed. Lactic acid not only lowers ph, but also functions as a factor causing increased permeability of the outer membrane of Gram-negative bacteria, thus it may increase the effectiveness of other antagonistic substances (Alakomi et al., 2000).

15 Comparison of Soya protein products: NattoGold 500 HP 300 SPC Glycinine < 100 ppm < 100 ppm or??? < 100 ppm β-conglycinine < 10 ppm < 10 ppm or 2 ppm < 10 ppm Lectins < 1 ppm < 1 ppm < 1 ppm Oligo-saccharides 1.00% 1.00% 1.5-4% Lactic acid 4.5 % - - ph

16 LACTIC ACID TEST

17 Beans Cleaning Cracking De-hulling Conditioning TVO Thai Vegetable Oil Co.,Th. Flaking Full Fat Flakes Oil Extraction Defatted Soy Flakes Oil Grinding Drying De-hulled Soybean meal

18 (Nattgold 500) MYCOTOXIN TEST

19 NattoGold 500 v.s MILK REPLACER: According to ELISA, the antigenic activity of one famous milk replacer from Netherlands is 11. We estimate it could be produced by: 1). 80% soy bean meal + 20% lactose 2). 70% soy bean meal + 30% whey ELISA = Enzyme Link Immunosorbent Assay

20 NattoGold 500 vs. MILK REPLACER: Value of milk replacer: 1). 80% soy bean meal + 20% lactose = = USD/Kg 2). 70% soy bean meal+30% whey = = USD/Kg

21 HOW TO REPLACE MILK REPLACER BY NattoGold 500 Same value: 70% NattoGold % whey Best: 50% NattoGold % whey

22 70% NattoGold % WHEY PROTEIN = =38.3% LACTOSE =21.0% LYSINE 2.40% M + C 1.16% THREONINE 1.60% TRYPTOPHAN 0.50% ANTIGEN LOW LACTOBACILLUS + MILK REPLACER 35 ~ 38 %???~ 20 % 2.5~ 2.6 % 1.10 % 1.60 % 0.40 %??? ---

23 NattoGold 500 WINS Fixed amount of NattoGold 500 mixed with fish meal in water,stand them for 24 hours NattoGold 500 NattoGold 500 NattoGold 500

24 NattoGold 500 WINS Fish meal : NattoGold 500 1:0 3:1 1:1 1:2 1:3 NO 2- (ppm) Fixed amount of NattoGold 500 mixed with fish meal in water,stand them for 24 hours. The NO 2- values of tests are 1/10 compared with blank.

25 USE OF NattoGold 500 Replace acidifiers, probiotics, intestinal bacteria depressing agents. Replace unnecessary drugs..

26 NattoGold 500 Free / low antigens content. The elimination of indigestible sugars. Rich in Vitamins & UGF. High digestibility(small particle nutrient). Best feed acidifier (4.5% lactic acid). Excellent probiotic(lactobacillus acidophilus). Fancy palatability. Reasonable price

27 Comparison of protein molecular weight in soy protein products Molecular weight (Daltons) NattoGold 500 HP-300 SPC Yeast Dehulled Soybean meal > % 34.36% 71.54% 4.24% 76.14% 12500~ ~ % 5.34% 8.68% 14.21% 2.02% 4.52% 23.10% 11.79% 32.51% 6.23% < % 37.20% 7.99% 49.04% 15.61% Food Industry Research and Development Institute, Taiwan

28 Molecule weight of Amino Acid Amino Acid Molecule Weight Amino Acid Molecule Weight Lysine 146 Valine 117 Methionine 149 Leucine 131 Cystine 121 Isoleucine 131 Threonine 118 Histidine 154 Tryptophan 211 Phenylalanine 169 Serine 105 Tyrosine 186 Arginine 175 Aspartic acid 133 Glycine 75 Glutamic acid 147 Alanine 89 Proline 115

29 High Quality Soya Protein Low antigen, High Lactic acid

30 BUREAU VERITAS CERTIFICATION OF ISO 9001:2008, GMP, HACCP

31 Evaluation of (NattoGold 500) (Dehulled Soybean Meal Fermentation by Lactobacillus) in Weaning Pigs Diets Jowaman Khajarern and Sarote Khajarern Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand Table 1. Variable ingredient by treatment diets fed during whole period of testing (35 days). Fish Meal Skimmed milk Soybean SPC* ESP 500 Dietary treatment replacer meal (55% CP) (38%CP) (55%CP) (48%CP) (44% CP) 1. Control SPC (NattoGold 500) SPC

32 T a b l e 2. C o m p o s i t i o n o f t h e e x p e r i m e n t a l w e a n i n g d i e t s. T 1 T 2 T 3 T 4 T 5 Ingredient Control SPC (NattoGold SPC (NattoGold 5.0% 500) 5.4% 10% 500) 11.4% Broken rice (8% CP) Skimmed milk replacer (38% CP) Whey powdered (11% CP) Fish meal (55% CP) Full fat soybean (38% CP) Soybean meal (44% CP) Soya protein (55% CP) (NattoGold 500) (min.49% CP) Crude rice oil Dicalcium phosphate

33 Table 3. Effect of (NattoGold 500) on average body weigh gain (ABWG), average daily gain (ADG), average daily feed intake (ADFI), feed efficiency (Feed : gain) and uniformity of body weight (UBW) at termination (21-56 days of age). Dietary ABWG ADG ADFI Feed:Gain UBW treatment kg g/d g/d g:g % b b b b ab ab ab a ab ab ab a ab ab ab ab a a a a Pooled SEM

34 Table 4. Summary on improvement performance of (NattoGold 500) supplementation in weaning pigs during days of age. Parameter T 1 Control T 2 SPC 5.0% T 3 (NattoGold 500) 5.7% T 4 SPC 10.0% T 5 (NattoGold 500) 11.4% Initial weight, kg/head Final weight, kg/head ABWG, kg/head Improvement performance : Improvement ADG, % Improvement ADFI, % Improvement Feed : Gain, % Improvement UBW, %

35 High Quality Soya Protein Low antigen, High Lactic acid