Studies of Egg-Shell Calcium (II) A Study on Absorption Rate of Egg-Shell Calcium in Rat

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1 J. Fd Hyg. Safety 18(2), (2003) ùƒe w (II) ùƒe w Á½ k w tœw Studies of Egg-Shell Calcium (II) A Study on Absorption Rate of Egg-Shell Calcium in Rat Sook Kyung Lee and Youn Tae Kim %FQBSUNFOUPG'PPE&OHJOFFSJOH%BOLPPL6OJWFSTJUZ$IPOBO,PSFB ABSTRACT This study was to investigate interaction between ionization rate and absorption rate of calcium (Ca) in each feeding Ca sources in rats. The results were as follows. 1. The ionized Ca ions fed into rats were absorbed in about two hours while Ca from other sources like powdered egg-shell Ca or precipitable Ca carbonate caused more than five hours to be absorbed. This means that the ionization of Ca is essential for the fast absorption in rat. 2. Absorption rate were increased in the rank order to brown rice vinegar-ca acetate > brewed vinegar-ca acetate > precipitated Ca carbonate > egg-shell Ca powder by feeding sources in rat and absorption rate of brown rice vinegar Ca was appeared 4 times highly than egg-shell Ca powder. 3. Absorption rate of brewed vinegar Ca acetate were appeared excellent, 1.4 times highly in case of the brewed vinegar at no ventilation condition than ventilation condition. 4. Ca concentration in blood serum was significantly enhanced the increased ionization rate of Ca in the above experiment rat models regardless of dietary Ca levels. Key words : ionization rate, absorption rate, brown rice vinegar Ca acetate, brewed vinegar Ca acetate, precipitated Ca carbonate, egg-shell Ca powder ƒ ƒw e ³x w œ 1) w» w e ƒ š w w ù. ƒœ t ƒw x y 2) w» w e ƒwš 3). w v e» w» w e t ù wš ù q š e» z ü w x û 4). e ƒw w e ƒ e y w ƒ w w š šwš 5-6). v 7) ƒ ü e w ƒ. y w ƒ ƒ e ƒw w e ƒw» Author to whom correspondence should be addressed. w. w e e z x w w š šw 8) ƒ e ƒ e w e 9). x e ùkü e w š e w š 10) e 11-13) š e w p ü w š 14-15) e w y e x ( ) w š. w x» z e ƒ wš ùƒe wš. s» ùƒ e y w w 16) w e x e» t ƒ wš ùƒe e k e 17) y e x 73

2 74 4PPL,ZVOH-FFBOE:PVO5BF,JN mw, y e x» šwš w. x w ùƒ w ü wš s»w w, w z w ùƒ e w qt 4% 4% x w. xw» w ùƒe w 18) e k e ( z y ) w w. x ùƒe ùƒe 16) w w. ùƒ e e y w ùkù 4% w ùƒ e ( w e w) 4% x w ùƒx e ( w x e w) w. x ww. e e d tœ x x w w x 19) ICP-MS(Model 7500A, Agilent, USA) w ƒƒ e w. e w e, x e ùƒe e k e e Ion-Meter (Model 920A ph/ise meter, Orion, USA) w Direct Measurement w 16). w e, x e ùƒe e k e tœ x w 20) w. Ÿ d UV-Visible Recording Spectrophotometer(Model UV-160A, Shimadzu, Japan) w. x x s³ 180 g 9 Sprague- Dawley f ( ) w j l w s³ 200 g w z 9 6 ù w. 22±2 o C w š, Ÿ»,» 12 w, 1 w š w 21-24). e k e x e, š k ùƒe e k e š e w w, ùƒe e k e e w w w ƒƒ 1 xkw w. w n ƒ x e w w 25-29). e d w w» w x x 24 k w w w,» x wš e, x e, ù ƒe e k e n w z 1 9 Õ l x 3z xw. w x Cold Lab Chamber(4 o C) 24 ew z 2500 rpm 20 w x. mw ¾ -20 o C þ w 30). x e x e þ w wwš TCA(Trichloroacetic acid) w z, LaCl 2 ƒ 1,000 ppm w 1N-HCl(LaCl 2 ) w w ICP-MS w ƒƒ x e w 16). m e m» y, ƒ x e x y w SAS program w ƒ x s ³ t r w š, p<0.05 ANOVA test z Duncan's multiple range test w w 31-36). š x d e e y e e (Calcium) y Table 1 5.0% (w/v) e ( s) 29.2±0.6mg/ml e 83.6± 1.8% y ùküš, 5.0%(w/v) e (m») 27.5±0.5 mg/ml e 85.2±1.5% y ùkü. w 5.0%(w/v) x e (m») 30.0±0.9 mg/ml e 87.2±1.3% y ùkü. w w ùƒe 958.0±1.6 mg/ ml e ùkþ ù y 0.04±0.02% û ùkü. e k e 400.8±1.3 mg/ ml e y 0.8±0.01% û ùk

3 4UVEJFTPG&HH4IFMM$BMDJVN ** 75 Table 1. Calcium concentration of dietary Sample for rat feeding test Type Calcium Concentration (mg/ml) Ionization Volume (mg/ml) Ionization Rate (%) 4% brewed vinegar* Ca acetate(no ventilation) 29.2Û Û Û1.8 4% brewage vinegar* Ca acetate(ventilation) 27.5Û Û Û1.5 4% brown rice vinegar* Ca acetate(ventilation) 30.0Û Û Û1.3 Egg-Shell Ca Powder 958.0Û Û Û0.02 Precipitated Ca Carbonate 400.8Û Û Û0.01 *Processing from 30 o C under 72 hour (vinegar volume is 200 ml) Table 2. Phosphorus concentration of dietary sample for rat feeding test Type Concentration of Phosphorus (elution volume, mg/ml) 4% brewage vinegar Ca acetate(no ventilation) % brewage vinegar Ca acetate(ventilation) % brown rice vinegar Ca acetate(ventilation) Egg-Shell Ca Powder Precipitated Ca Carbonate û. ùƒe e 50% û ù y 2 ù ùkü. e e w (Phosphorus) w š. p ƒ e 1:1~2 w e ƒ 37). š 38) w e 1.2% Ca : 0.5% P e ƒ š šw w e w š šw. w e e e w» w w Table 2 ùkü. e x e ƒƒ mg/100 g, ùƒe mg/100 g, e k e mg/100 g w ùkû. w x w e e e ( s) 12.92% Ca : % P, e (m») 27.5% Ca : % P, x e (m») 30.0% Ca : % P, ùƒe 95.8% Ca : % P, e k e 40.0% Ca : % P ùkû. w e e w e w. x w x ü e x w» s³ 200 g w 2 w x ü e w 8.79±1.27 mg/ dl ùkû x e ƒ 7.2~13.9 mg/dl ew x w k. Fig. 1. Concentration of calcium in rat's plasma as a result of feeding calcium materials. e ( s) z x e y e ( s) x e y Fig. 1 n z ƒw ƒ 2 z» e w 2.67 ± 1.2 mg/dl j ƒw ùkû (p<0.05). y» ƒ z 4 z» e ƒ¾ w ù 5 z l 8 ¾ w ƒw ƒ 9 z» w w ùkû. e (m») z x e y e (m») x e y Fig. 1 s n z ƒw ƒ 2 z» e w 3.18 ± 0.34 mg/dl j ƒw ùkû (p<0.05). y» ƒ z 4 z» e

4 76 4PPL,ZVOH-FFBOE:PVO5BF,JN û w ù ƒw 6 z x e š w w ƒ 9 z» w w ùk û. x e (m») z x e y x e (m») x e y (Fig. 1) n 2 z» e w 3.51 ± 0.46 mg/dl j ƒw ùkû (p<0.05). y» ƒ z 5 z» e w w ƒ 7 ¾ w ƒw ƒ 9 z» w w ùkû. ùƒe z x e y ùƒe x e y Fig. 2 n z w ƒw ƒ 5 z» e w 3.35 ± 0.24 mg/dl j ƒ w še ùkü (p<0.05). z w w ƒ 9 z» w w ùkû. e k e z x e y e k e x e y Fig. 2 n z w ƒw ƒ 5 z» e w 3.11 ± 1.36 mg/dl j ƒw še ùkü (p<0.05). z w w ƒ 9 z» w w ùkû. e x ƒ x x» e x Table 3 x e ƒ ùkû e ( s), e (m»), e k e, ùƒe û ùkù y ew. e s m» ƒ w x e e (m») 1.86, e ( s) 1.36, e x ùkû. ùƒe e k e y ƒ j ùkü (p<0.05). e x x e w ùƒe 28.96%, e k e 43.33% ƒ û ùkû. ù y x e, e ( s) e (m») ƒ j ùkû. e x y e y û e ùkü e x e w w e y w w. Fig. 2. Concentration of calcium in rat's plasma as a result of feeding egg-shell calcium powder and precipitated calcium carbonate w w w. Table 3. Concentration of total calcium in plasma during test period Type Concentration of Total Calcium in Plasma (mg/dl) 4% brewed vinegar Ca acetate(no ventilation) ± 2.53 a) 4% brewed vinegar Ca acetate(ventilation) a, c) ± % brown rice vinega Ca acetate(ventilation) ± 3.63 b) Egg-Shell Ca Powder ± 1.23 c) Precipitated Ca Carbonate ± 1.44 c) a), b), c), 1), 2), 3) Means with the same letter in row are not significant at P<0.05 level by Duncan's multiple range test.

5 4UVEJFTPG&HH4IFMM$BMDJVN ** 77 ùƒe e w w e, x e ùƒe e k e z e x ( ) w x. 1. y e ( s, m») x e n 2 z še ùkû y û ùƒe ù e k e 5 z še ùkû. y e ƒ w. 2. w e e x x e > e ( s)> e (m»)>e k e >ùƒe û ùkû. y. 3. x e x e ƒ ùƒe w 4 ùkù e x e w w e y w. 4. e e x s m» w 1.4 ùkù ³ w» w ƒ w w. š x 1. Hong, S.M. and Kim, H.J.: Calcium status and Effect of Nutrition of Prevention Osteoporosis in Middle-aged Women. Journal of the Korean Dietetic Association, 7(2), (2001). 2. : t k, ƒ (2002) : yw, y (1994). 4. : 98, (1999). 5.,, x : w. e e w. w wz, 24(2), (1991). 6. Smith, K.T. and Henders, S.M.: Calcium absorption from a new calcium delivery system(cc). Calcif. Tissues Int.. 41, (1987). 7. Pie, J.E. and Paik, H.Y.: The effect of meat protein and soy protein on calcium metabolism in young adult korean women, Korean J. Nutr., 19(1), (1986). 8. Jung, S.H. and Choi, M.J.: Effect of dietary protein level on Ca efficiency in bone mineral density in growing rats, Korean J. Nutr., 28(9), (1995). 9. Chang, Y.E., Chung, H.K., Chang, N.S. and Lee, H.S.: The effects of dietary protein and calcium levels on calcium and bone metabolism in growing rats, Korean J. Nutr., 30(3), (1997). 10. O, J.H., Song, M.H. and Lee, Y.S.: Effects of high protein and calcium intakes on calcium metabolism and renal function in ovariectomized osteoporosis rat model, Korean J. Nutr., 30(6), (1997). 11. Chung, H.K., Chang, N.S., Lee, H.S. and Chang, Y.E.: The effect of various types of calcium sources on calcium and bone metabolism in rats, Korean J. Nutr., 29(5), (1996). 12. Lee, Y.S. and O, J.H.: Effects of bovine bone ash and calcium phosphate on calcium metabolism in postmenopausal osteoporosis model rats, Korean J. Nutr., 28(5), (1995). 13. Lee, S.H. and Chang, S.O.: Comparison of the bioavailability of calcium from anchovy, tofu and nonfat dry milk(nfdm) in growing male rats, Korean J. Nutr., 27(5), (1994). 14. Lee, S.H., Hwangbo, Y.S., Kim, J.Y. and Lee, Y.S.: The Bioavailability of Dietary Calcium Sources, The Korea Nutrition Society, 30(5), (1997). 15. Kim, J.S., Choi, J.D. and Kim, D.S.: Preparation of calciumbased powder from fish bone and its characteristics, J. Agri. Chem. Biotechnology, 41(2), (1998). 16. Lee, S.K. and Park, J.H.: Studies of egg-shell calcium(i) The effects of elution condition of egg-shell calcium on elution quantity and ionization rate, J. Fd Hyg. Safety 17(4), (2002). 17.», y, ½ ³: e k e, ywœ», 12(5), (1994). 18. Shin, H.S. and Kim, K.H.: Preparation of calcium powder from eggshell and use of organic acid for enhancement of calcium ionization, J. Agri. Chem. Biotechnology, 40(6), (1997). 19. t t : tœ ( ),, (2000). 20. t t : tœ ( ),, 272, 274 (2000). 21. Lee, S.H., Hwangbo, Y.S., Kim, J.Y. and Lee, Y.S.: A study on the bioavailability of dietary calcium sources, Korean J. Nutr., 30(5), (1997). 22. Sung, C.J.: Effects of calcium intake on Calcium, sodium and potassium metabolism in young and adult female rats, Korean J. Nutr., 28(4), (1995). 23. Kim, K.H., Choi, M.J. and Lee, I.K.: The effect of dietary calcium level on bone mineral density and bone mineral content in ovariectomized female rats, Korean J. Nutr., 29(6), (1996).

6 78 4PPL,ZVOH-FFBOE:PVO5BF,JN 24. Chio, M.J. and Jo, H.J.: Effect of chloride suppliementation on bone metabolism in rats consuming a low calcium diet, Korean J. Nutr., 29(10), (1996). 25. Do, J.C. and Huh, R.S.: Changes of the blood chemistry, lipid and protein components in blood and liver tissue according to the time lapsed of the rat after aral administration of caffeine, Korean J. Vet. Res., 36(4), (1996). 26. Do, J.C: Determination of sulfamethazine residues in liver, kidney and muscle according to the time lapsed after oral administration of sulfamethazine sodium to rats, Korean J. Vet. Res., 36(3), (1996). 27. Choi, M.D., Kim, D.H., Kim, J.H. and Kim, S.H.: Short-term oral toxicity test of the pine needle extracts in rat, Korean J. Food Sci. Technol., 5, (1999). 28. Park, R.J., Kim, N.J., Lee, K.T. and Seo, S.H.: Comparative studies on concentration of decursinol in plasma after oral administration of angelicae gigantis radix extract and combined use of decursin and cnidii rhizoma extract or bupleuri radix extract in rats, Kor. J. Pharmacogn., 32(1), (2001). 29. Park, S.C. and Yun, H.I.: Bioavailability and comparative pharmacokinetics of two enrofloxacin formulations in broiler chickens, Korena J. Vet Clin Med., 14(2), (1997). 30. O, J.H. and Lee, Y.S.: Effects of dietary calcium levels on the reduction of calcium availability in ovariectomized osteoporosis model rats, Korean J. Nutr., 26(3), (1993). 31. Chang, S.H., Cho, S.Y. and Park, M.L.: Effect of calcium and magnesium on the lipid and mineral composition of serum and tissues in cholesterol-fed rats, J. Korean Soc. Food Nutr., 17(2), (1988). 32. Lee, K.H., Choi, I.S., Lee, S.S., Oh, S.H.: Effects of nondigestable substances and calcium on lipid metabolism in rats, J. Korean Soc. Food Sci. Nutr., 26(5), (1997). 33. Lee, S.H. and Lee, Y.S.: Effects of late-harvested green tea extract on lipid metabolisn and ca absorption in rats, Korean J. Nutr., 31(6), (1998). 34. Lee, J.S. and Cho, S.Y.: Effects of dietary protein and calcium levels on Ca, Fe, Cu, Zn and Mg level of the tissues of the Pbadministered rats, J. Korean Soc. Food Nutr., 22(2), (1993). 35. Lee, J.S., Hong, H.O. and Yu, C.H.: A study on the effect of caffeine intake on calcium and phosphorus metabolism in ovariectomized rats, Korean J. Nutr., 29(9), (1996). 36., : m, e, (1998). 37. : v, ƒ (2002). 38. Jeong, H.K., Kim, J.Y., Lee, H.S. and Kim, J.Y.: The effect of dietary calcium and phosphate level on calcium and bone metabolism in rats, Korean J. Nutr., 30(7), (1997).