Public Analyst s Laboratory

Transcription

1 Public Analyst s Laboratory Sir Patrick Dun s, Lower Grand Canal Street, Dublin 2 Testing Laboratory Registration number: 099T is accredited by the Irish National Board (INAB) to undertake testing as detailed in the Schedule bearing the Registration Number detailed above, in compliance with the International Standard ISO/IEC 17025: nd Edition General Requirements for the Competence of Testing and Calibration Laboratories (This Certificate must only be read in conjunction with the Annexed Schedule of ) Date of award of accreditation: 19:12:2002 Date of last renewal of accreditation: 11:10:2012 Expiry date of this certificate of accreditation: 11:10:2017 This shall remain in force until further notice subject to continuing compliance with INAB accreditation criteria, ISO/IEC and any further requirements specified by the Irish National Board. Manager: Chairperson: Dr Adrienne Duff Mr Tom O Neill Issued on 11 October 2012 Organisations are subject to annual surveillance and are re-assessed every five years. The renewal date on this Certificate confirms the latest date of renewal of accreditation. To confirm the validity of this Certificate, please contact the Irish National Board. The INAB is a signatory of the European co-operation for (EA) Testing Multilateral Agreement (MLA) and the International Laboratory Cooperation (ILAC) Mutual Recognition Arrangement. Edition 29, 09/07/ T Page 1 of 35

2 Schedule of Wilton Park House, Wilton Place, Dublin 2, Ireland Tel Fax inab@inab.ie Web (Annex to Certificate) Chemical and Biological Testing Laboratory Initial Registration Date : 23-September-1998 Postal Address: Sir Patrick Dun s, Lr. Grand Canal Street, Dublin 2 (Address of other locations as they apply) Telephone: +353 (1) Fax: +353 (1) Contact Name: Rosemary Hayden Facilities: Public testing service Edition 29, 09/07/ T Page 2 of 35

3 Wilton Park House, Wilton Place, Dublin 2, Ireland Tel Fax inab@inab.ie Web Schedule of THE IRISH NATIONAL ACCREDITATION BOARD (INAB) is the Irish body for the accreditation of organisations including laboratories. Laboratory accreditation is available to testing and calibration facilities operated by manufacturing organisations, government departments, educational institutions and commercial testing/calibration services. Indeed, any organisation involved in testing, measurement or calibration in any area of technology can seek accreditation for the work it is undertaking. Each accredited laboratory has been assessed by skilled specialist assessors and found to meet criteria which are in compliance with ISO/IEC or ISO/IEC (medical laboratories). Frequent audits, together with periodic inter-laboratory test programmes, ensure that these standards of operation are maintained. Testing and Calibration Categories: : Permanent laboratory calibration and testing where the laboratory is erected on a fixed location for a period expected to be greater than three years. Category B: Category C: Category D: Site calibration and testing that is performed by staff sent out on site by a permanent laboratory that is accredited by the Irish National Board. Site calibration and testing that is performed in a site/mobile laboratory or by staff sent out by such a laboratory, the operation of which is the responsibility of a permanent laboratory accredited by the Irish National Board. Site calibration and testing that is performed on site by individuals and organisations that do not have a permanent calibration/testing laboratory. Testing may be performed using (a) (b) (c) (d) portable test equipment a site laboratory a mobile laboratory or equipment from a mobile or site laboratory Standard Specification or Test Procedure Used: The standard specification or test procedure that is accredited is the issue that is current on the date of the most recent visit, unless otherwise stated. Glossary of Terms Facilities: Public calibration/testing service: Conditionally available for public calibration/testing: Normally not available for public calibration/testing: Commercial operations which actively seek work from others. Established for another primary purpose but, more commonly than not, is available for outside work. Unavailable for public calibration/testing more often than not. Laboratory users wishing to obtain assurance that calibration or test results are reliable and carried out to the Irish National Board criteria should insist on receiving an accredited calibration certificate or test report. Users should contact the laboratory directly to ensure that this scope of accreditation is current. INAB will, on request, verify the status and scope. Edition 29, 09/07/ T Page 3 of 35

4 Plastics Chemical analysis Primary aromatic amines in black nylon kitchen utensils: 2,4 toluenediamine (2,4-TDA) 2,6-toluenediamine (2,6-TDA) ppb Aniline (ANL) 4,4' Methylenedianiline (4,4'-MDA) ,000 ppb (analysed as 3% acetic acid solution, results obtained must be corrected for the surface area of the individual utensil under analysis) Equipment/techniques used SOP PALC 0092 method based on: Mortensen, S.K.; Trier, X.T; Foverskov, A; Petersen, J.H: Specific determination of 20 primary aromatic amines in aqueous food simulants by liquid chromatography electrospray ionizationtandem mass spectromtery, J. Chromatogr. A 1091, (2005) Residual formaldehyde in melamine kitchenware 3-30 mg/kg food simulant (analysed as 3% acetic acid solution) Results obtained must be corrected for the surface area of the individual utensil under analysis. SOP PALC 0117 based on Determination of formaldehyde in food simulants I.S. CEN/TS :2005 Edition 29, 09/07/ T Page 4 of 35

5 Plastics Chemical analysis Residual melamine in melamine kitchenware mg/kg food simulant (analysed as 3% acetic acid solution). Results obtained must be corrected for the surface area of the individual utensil under analysis Equipment/techniques used SOP PALC 0094, based on I.S.EN :2004, Waters application note EN, Oct Chemical analysis Epoxidised soybean oil (ESBO) in PVC Gasket 3.0% - 50% w/w In house method SOP PALC 0039 Edition 29, 09/07/ T Page 5 of 35

6 Foods Cereals and bakery products. ) Foods Wine Alcoholic beverages (other than wine).10 Non-alcoholic beverages Cumarin by HPLC with U detection. Bakery products: mg/kg Breakfast cereals: 2-50 mg/kg Alcohol by volume in drinks by distillation and pyknometry 4-50% v/v Fructose, glucose and sucrose by HPLC with refractive index detection SOP PALC 0121 based on Anal methods 2011, 3, 414. Scotter et al. SOP PALC 0001 method based on: Leatherhead F.R.A. Analytical Methods Manual (2nd Edition) Determination of Ethyl Alcohol by Distillation SOP PALC 0005 method.12 Alcoholic beverages Spirits Fructose % w/v Glucose % w/v Sucrose % w/v Fructose, glucose and sucrose by HPLC with electrochemical detection SOP PALC 0005 method Fructose mg/l Glucose mg/l Sucrose mg/l Edition 29, 09/07/ T Page 6 of 35

7 Foods Confectionery Honey Fructose, glucose and sucrose in honey % w/w SOP PALC 0005 method Diastase number of honey 5-16 Diastase number SOP PALC 0113, by Phadebas, based on Harmonised Methods of the International Honey Commission, Conductivity of Honey ms.cm -1 SOP PALC 0114, as above Honey Commission, ph of honey Acidity of honey 5-50 meq/kg SOP PALC 0115, as above, Honey Commission, Insoluble matter in honey g/100g SOP PALC 0118, as above Honey Commission, Moisture in honey 10-30% SOP PALC 0086 as above Honey Commission, hydroxymethylfurfural (HMF) content of honey by HPLC with UV detection mg/kg SOP PALC 0057 as above Honey Commission, Edition 29, 09/07/ T Page 7 of 35

8 Foods Non-alcoholic beverages Benzoic acid and sorbic acid in non-alcoholic beverages by HPLC Benzoic acid mg/l Sorbic acid mg/l SOP PALC 0008 based on VEMS Method, Code: F/0290, June, 1994 Dairy products Benzoic acid and sorbic acid in.05 Fats and Oils foods by steam distillation and SOP PALC Soups broths and HPLC sauces Benzoic acid mg/kg method based on: VEMS.07 Cereals & bakery Sorbic acid mg/kg Method, Code: F/0290, products June, Fruit and vegetables.15 Confectionery.03 Meat and meat Sulphur dioxide in food and SOP PALC 0011 products, game and beverages by distillation and poultry titrimetry method based on: VEMS.04 Fish, Shellfish and Method, Code: F/0360, May molluscs Meat products mg/kg Fruit and vegetables Dried fruit mg/kg.10 Non-alcoholic Wine mg/l beverages Raw potatoes mg/kg.11 Wine Raw crustaceans mg/kg.12 Alcoholic beverages Cider mg/l (other than wine) Cordials mg/l.15 Confectionery Edition 29, 09/07/ T Page 8 of 35

9 Foods Fruit and vegetables.10 Non-alcoholic beverages Nitrate in lettuce and spinach by anion exchange HPLC mg/kg Aspartame, acesulfame-k and saccharin in non-alcoholic beverages by HPLC Aspartame mg/l Acesulfame-K mg/l Saccharin mg/l SOP PALC 0015 method based on: I.S. EN :1997 SOP PALC 0016 method based on: HPLC in Food Analysis, Ed. R. Macrae, 2nd Edition, 1988, P Steviol Glycosides (Rebaudioside A and Stevioside) in flavoured drinks. Rebaudioside A: mg/l ( mg/l steviol equivalents) Stevioside: mg/l (4-160 mg/l steviol equivalents) SOP PALC 0135 based on Steviol Glycosides, Prepared at the 73 rd JECFA (2010). Dairy products Aspartame, acesulfame-k and SOP PALC Soups, broths and saccharin in selected foods by Documented in house sauces HPLC method based on:.13 Ices and desserts Aspartame mg/kg I.S. EN 12856: Confectionery Acesulfame-K mg/kg Saccharin mg/kg Edition 29, 09/07/ T Page 9 of 35

10 Foods Fish, shellfish and molluscs Fish, shellfish and fish products Soups (fish), broths and sauces Tyramine, putrescine, cadaverine, histamine, agmatine, phenyethylamine, spermidine and spermine by HPLC and fluorescence detection Tyramine mg/kg (.04) mg/kg (.06) Putrescine mg/kg (.04) mg/kg (.06) Cadaverine mg/kg (.04) mg/kg (.06) Histamine mg/kg (.04) mg/kg (.06) Agmatine mg/kg (.04) mg/kg (.06) Phenyethylamine mg/kg (.04) mg/kg (.06) Spermidine mg/kg (.04) mg/kg (.06) Spermine mg/kg (.04) mg/kg (.06) SOP PALC 0017 method based on: SOP for Biogenic Amines by HPLC, Torry Research Station, MAFF, Scotland.10 Non-alcoholic Caffeine in foodstuffs by HPLC SOP PALC 0025 beverages and UV detection Documented In house.14 Cocoa and Cocoa Ground Coffee g/kg method:based on ISO preparations, coffee, Liquid Samples mg/l 10095:1992(E) tea. Edition 29, 09/07/ T Page 10 of 35

11 Foods Dairy products Fruit and vegetables Non-alcoholic beverages Alcoholic beverages (other than wine) Ices and desserts Confectionery.10 Non-alcoholic beverages Apple Juice Apple smoothies Sucralose in foodstuffs by HPLC and refractive index detection Non-alcoholic beverages mg/l Yoghurts mg/kg Patulin ug/kg SOP PALC 0026 Documented In house method based on TDS for Splenda, Tate and Lyle SOP PALC 0045 documented in house method based on Romer Application brief, 2 nd Feb LC-MS/MS confirmation..03 Meat and meat Nitrite and nitrate (expressed SOP PALC 0028 products, game and as sodium nitrite and sodium poultry nitrate) by anion exchange method based on: I.S. EN.21 Others HPLC :2005 Brines mg/kg (.03) mg/kg (.21, Nitrate) mg/kg (.21, Nitrite).18 Foodstuffs intended for special nutritional use infant formula and follow on formula Taurine by HPLC with UV detection Infant and follow on formula prepared as per manufacturer s instructions Range mg/l SOP PALC 0064 method based on: J Chem Ed 78 (6) 2001 Edition 29, 09/07/ T Page 11 of 35

12 Foods Meat and meat products, game and poultry Smoked meat Fish, shellfish and molluscs Smoked fish Fats and oils Herbs and spices Cocoa and Cocoa preparations, coffee, tea Foodstuffs intended for special nutritional uses Infant formula baby foods Others Food supplements Polycyclic aromatic hydrocarbons: Cyclopenta[cd]pyrene Benzo[a]anthracene Chrysene 5-Methylchrysene Benzo[b]fluoranthene Benzo[j]fluoranthene Benzo[k]fluoranthene Benzo[a]pyrene Indeno[1,2,3-cd]pyrene Dibenzo[a,h]anthracene Benzo[ghi]perylene Dibenzo[a,l]pyrene Dibenzo[a,e]pyrene Dibenzo[a,i]pyrene Dibenzo[a,h]pyrene Fats and oils: µg/kg Infant formula: µg/kg Herbs and spices: µg/kg Smoked meat & fish: µg/kg Food supplements: µg/kg Brewed beverages: µg/kg Raw beverage: µg/kg Baby foods: µg/kg Heat treated meat/meat products ug/kg Cocoa beans and derived products ug/kg (fat) Equipment/techniques used method by GC-MS SOP PALC 0075 Edition 29, 09/07/ T Page 12 of 35

13 752 Residues in foods and agricultural materials Elements Arsenic species in fish tissue by HPLC- ICP/MS Inorganic arsenic mg/kg Equipment/techniques used methods: SOP PALC 0108, based on US Environmental Protection Agency methods 3110 and Other Residues Acrylamide in food µg/kg SOP PALC 0032 Edition 29, 09/07/ T Page 13 of 35

14 Residues in foods and agricultural materials Mycotoxins Cereals, nut products, dried fruit and dried fruit products products, shelled nuts, nuts, groundnuts, spices, seeds and baby foods.05 Mycotoxins Maize-based foods and baby foods Aflatoxins B 1, B 2, G 1 and G 2 in food by immunoaffinity column extraction and HPLC Cereals, seeds, nut products, dried fruit and dried fruit products: µg/kg Shelled nuts µg/kg Nuts and groundnuts in shell µg/kg Spices µg/kg Baby foods µg/kg (B 1 only) Fumonisin B 1, B 2 and B 3 in maize and maize products by immunoaffinity column extraction and HPLC Fumonisin B µg/kg Fumonisin B µg/kg Fumonisin B ug/kg Total Fumonisins ,190 µg/kg methods: SOP PALC 0031 SOP PALC 0076 method based on Application notes from R- Biopharm Rhone Ltd. Edition 29, 09/07/ T Page 14 of 35

15 752 Residues in foods and agricultural materials Milk powder Milk Aflatoxin M 1 in milk and milk powder by HPLC and fluorescence detection. Milk powder µg/kg Milk µg/l SOP PALC 0077 method based on Application notes from R- Biopharm Rhone Ltd..05 Mycotoxins Cereal products Dried fruits Wine Beer Coffee Baby food Liquorice Spices Grape juice Ochratoxin A in foodstuffs by high performance liquid chromatography (HPLC) and fluorescence detection Cereals, Coffee, Dried fruit, Paprika, Chocolate, Chilli, Liquorices, Black/White pepper, nutmeg, ginger, tumeric 1-60 µg/kg Baby foods 0-30 µg/kg Red/White grape juice, Beer and wine 1-6 µg/l, Cocoa 1-60ug/kg, Mixed spices 1-60 ug/kg SOP PALC 0018 method based on Application notes from R- Biopharm Rhone Ltd. Edition 29, 09/07/ T Page 15 of 35

16 Residues in foods and agricultural materials Mycotoxins Cereals, Cereal-based baby foods Tinned sweetcorn.05 Mycotoxins Cereals, cereal based baby food, pasta Zearalenone in foodstuffs by immunoaffinity column extraction and HPLC with fluorescence detection Cereals μg/kg Cereal-based baby foods μg/kg Tinned sweetcorn µg/kg Deoxynivalenol by HPLC and fluorescence detection 50-4,000 µg/kg SOP PALC 0022 method based on Application notes from R- Biopharm Rhone Ltd. SOP PALC 0081 method based on Application notes from R- Biopharm Rhone Ltd..05 Mycotoxins Cereals T-2 and HT-2 toxins T ug/kg HT ug/kg Sum of T-2 and HT ug/kg. SOP PALC 0074 method. UPLC- MS/MS Edition 29, 09/07/ T Page 16 of 35

17 Residues in foods and agricultural materials Other residues.99 Sweets, Biscuits and cakes Soy products, milk powder Furan in food by headspace GC- MS Solid foods furan (µg/kg) 5-10,000 Liquid foods Furan (µg/l) Melamine in foodstuffs mg/kg for sweets biscuits and cakes mg/kg for soy products, milk powder method: SOP PALC 0041 Based on U.S. Food and Drug Administration (US FDA) Centre for Food Safety and Applies Nutrition (CFSAN) Determination of furan in foods May ~dms/furan.html method: SOP PALC 0091 based on Waters application note EN.99 Other residues jarred foods including infant foods Epoxidised Soybean Oil in food, food simulant mg/kg ESBO in food mg/kg ESBO in food simulant method: SOP PALC 0039, documented in house method based on Castle, L., Sharman, M., and Gilbert, J. A.O.A.C. No.6., 71, (1988) Edition 29, 09/07/ T Page 17 of 35

18 766 Waters Waters for potable Iron in water samples by flame SOP PALCW 0001 and domestic purposes a.a. spectrophotometry In house method based on (routine method) with the examination of water microwave digestion as and waste water, 22nd necessary edition; 2012 Fe μg/l Mercury in Water by Cold Vapour AA spectrophotometry µg/l Edition 29, 09/07/ T Page 18 of 35

19 766 Waters Waters for potable and domestic purposes Automated colorimetric/turbidimetric analysis: Ammonium (as NH 4 ) mg/l Chloride (Cl) mg/l Nitrite (NO 2 ) mg/l Nitrate (NO 3 ) mg/l Sulphate (SO 4 ) 8-250mg/l Alkalinity (HCO 3 ) mg/l Total Hardness (CaCO 3 ) mg/l Colour (Pt-Co units) mg/l SOP PALCW 0021 using Thermoscientific Aquakem 250 discrete analyser manual Edition 29, 09/07/ T Page 19 of 35

20 INAB Classification number (P9) 766 Waters Waters for potable and domestic purposes Waters for potable and domestic purposes Fluoride and sulphate in water by reagent free ion chromatography (RFIC) Sulphate mg/i Fluoride mg/i Total metals in water samples by inductively coupled plasma/mass spectrometry (ICP-MS) Chromium 4-80 Cr µg/l Cadmium 2-40 µg/l Lead 2-40 µg/l Nickel 2-40 µg/l Copper mg/l Sodium mg/l Calcium mg/l Potassium mg/l Magnesium mg/l Aluminium µg/l Antimony 2-40 µg/l Arsenic 2-40 µg/l Selenium 2-40 µg/l Manganese µg/l Boron µg/l SOP PALCW 0005 method based on the standard method for the examination of waters and waste waters 22 nd edition, 2012, sections 4110 Determination of Anions by IC method SOP PALCW 0006 Edition 29, 09/07/ T Page 20 of 35

21 766 Waters Waters for potable and domestic purposes Turbidity in waters Turbidity (NTU) Determination of ph 4-10 Determination of conductivity µS/cm at 20 C SOP PALCW 0020 method based on Hach Turbidimeter Method. SOP PALCW 0022 in house method based on Jenway ph meter operation SOP PALCW 0019 in house method based on Jenway conductivity meter operation General chemistry.09 Trace elements Hg in blood by cold vapour AA spectrometry. method µg/l SOP PALC 0085, based also on SOP PALC Copper in serum by flame AA spectrophotometry. method µg/100ml SOP PALC 0099 Zinc in serum by flame AA spectrophotometry. method µg/100ml SOP PALC 0101 Copper in urine by flame AA spectrophotometry. method µg/100ml SOP PALC 0104 Edition 29, 09/07/ T Page 21 of 35

22 Misc Materials and products Chemical tests Analysis of metals in Hexafluorosicicic Acid solution (HFSA) Using ICP-MS Antimony µg/l Arsenic µg/l Cadmium µg/l Chromium µg/l Lead µg/l Nickel µg/l Selenium µg/l SOP PALCW 0006 based on I.S. EN 12175:2013 Analysis of Flouride in 10.9% HFSA solution by Reagant free Ion Chromatography SOP PALCW 0005 based on I.S. EN 12175:2013 Analysis of Mercury by Cold Vapour Atomic Absorption spectrophotometry µg/l Analysis of HFSA in Aqueous solution (10-35%) by Titrimetry SOP PALCW 0023 based on I.S. EN 12175:2013 SOP PALCW 0024 based on I.S. EN 12175:2013 Edition 29, 09/07/ T Page 22 of 35

23 Biological Testing Laboratory Microbiological tests on food Dairy products Egg and egg products Meat and meat products, game and poultry Fish, shellfish and molluscs Fruit and vegetables Prepared dishes Aerobic colony count (pour plate) at 30 C for 72 hours. Aerobic colony count (spiral plate) at 30 C for 48 hours. SOP PALM 0001 based on ISO 4833:2003 SOP PALM 0001 (S) based on ISO 4833:2003 Edition 29, 09/07/ T Page 23 of 35

24 Public Analyst's Laboratory Biological Testing Laboratory Microbiological tests on foods Egg and egg products Meat and meat products, game and poultry Fish, shellfish and molluscs Cereals and bakery products Cocoa and cocoa preparations, coffee and tea Prepared dishes Enumeration of β- glucuronidase- positive E.coli by colony count at 44ºC using TBX SOP PALM 0026 based on ISO :2001 Dairy products Enumeration of presumptive SOP PALM 0003(S) based on.02 Egg and egg products Bacillus cereus using Bacillus ISO 7932: Meat and meat sereus agar. products, game and poultry.04 Fish, shellfish and molluscs.07 Cereals and bakery products.08 Fruit and vegetables.17 Prepared dishes Edition 29, 09/07/ T Page 24 of 35

25 Public Analyst's Laboratory Biological Testing Laboratory Microbiological tests on food Fish, shellfish and molluscs Detection and enumeration of Vibrio parahaemolyticus (Surface spread/spiral) SOP PALM 0028 based on ISO TS :2007/Cor1:2008 Dairy products Enumeration of Clostridium SOP PALM 0006 based on ISO.02 Egg and egg products perfingens 7937: Meat and meat products, game and poultry.04 Fish, shellfish and molluscs.08 Fruit and vegetables.17 Prepared dishes Edition 29, 09/07/ T Page 25 of 35

26 Public Analyst's Laboratory Biological Testing Laboratory Microbiological Tests on food Dairy products Egg and egg products Meat and meat products, game and poultry Fish, shellfish and molluscs Fruit and vegetables Prepared dishes Enumeration of Enterobacteriacae SOP PALM 0009 based on ISO : Egg and egg products Enumeration of coagulase- SOP PALM 0061 based on ISO.03 Meat and meat positive staphylococci by RPF : 1999 Amd products, game and technique poultry.04 Fish, shellfish and molluscs.08 Fruit and vegetables.17 Prepared dishes Edition 29, 09/07/ T Page 26 of 35

27 Public Analyst's Laboratory Biological Testing Laboratory Microbiological tests on food Dairy products Egg and egg products Meat and meat products, game and poultry Fish, shellfish and molluscs Cereals and bakery products Fruit and vegetables Prepared dishes Detection of Listeria monocytogenes Enumeration of viable aerobic mesophilic flora using Tempo TVC. (excluding.08 Fruit and vegetables) SOP PALM 0017 based on ISO :1996/Amd.1:2004 SOP PALM 0079 based on AFNOR TEMPO TVC validation BIO 12/15-09/05 (Renewed 2009).02 gg and egg products.03 Meat and meat method: products, game and Elfa Detection of Salmonella SOP PALM 4001 based on poultry spp using VIDAS SLM Kit. AFNOR VIDAS Salmonella.04 Fish, shellfish and (Vidas SLM) method BIO molluscs 12/1-04/94 (renewed 2010).08 Fruit and vegetables Screening method. Cultural.17 Prepared dishes and confirmation aspects.06 Soups, broths and based on ISO 6579:2002 sauces Amd Herbs and spices.12 Alcoholic beverages Detection of salmonella spp SOP PALM 0004 based on (other than wine) ISO6579:2002 Amd Cream Liquers.15 Confectionery Edition 29, 09/07/ T Page 27 of 35

28 Public Analyst's Laboratory Biological Testing Laboratory 811 Microbiological Tests on Food method: Dairy Products Elfa Detection of SOP PALM 4003 based on.02 Egg and egg products Campylobacter spp using VIDAS AFNOR VIDAS Campylobacter.03 Meat and meat CAM kit. method (VVIDAS CAM) BIO products, game and 12/29-05/10 Screening poultry method. Cultural and.04 Fish, shellfish and confirmation aspects based molluscs on ISO : Fruit and vegetables.09 Herbs and Spices.17 Prepared dishes Detection of Campylobacter SOP PALM 0023 based on ISO spp :2006 Dairy products Enumeration of Listeria spp and SOP PALM 0018(S) based on.02 Egg and egg products L. monocytogenes. ISO Meat and meat 2:1999/Amd.1:2004 products, game and poultry.04 Fish, shellfish and molluscs.08 Fruit and vegetables.17 Prepared dishes Edition 29, 09/07/ T Page 28 of 35

29 Public Analyst's Laboratory Biological Testing Laboratory Microbiological Tests on Food Egg and egg products Meat and meat products, game and poultry Fish, shellfish and molluscs Cereals and Bakery products Prepared dishes Enumeration of Escherichia coli in food products using TEMPO EC(E coli) test Documented in house method SOP PALM 0005, AFNOR TEMPO EC Validation BIO 12/13-02/05 (Renewed 2009) Edition 29, 09/07/ T Page 29 of 35

30 Public Analyst's Laboratory Biological Testing Laboratory Waters including effluents Bacteriological condition of potable waters Bacteriological condition of environmental waters Detection and enumeration of coliforms bacteria and E.coli in water by membrane filtration method: SOP PALM 0100 based on the Microbiology of Drinking Water 2009, Part 4A.11 Bacteriological Enumeration of heterotrophic SOP PALM 0107 based on the condition of potable bacteria colony count Microbiology of Drinking waters technique at 22 C or 37 C Water 2012, Part 7.15 Bacteriological condition of swimming Detection and enumeration of SOP PALM 0106 based on the pools and spas Ps. aeruginosa in water by Microbiology of Drinking membrane filtration Water 2010, Part 8 Edition 29, 09/07/ T Page 30 of 35

31 Public Analyst's Laboratory Biological Testing Laboratory 870 Waters including effluents method:.11 Bacteriological Detection and enumeration of SOP PALM 0102 based on ISO condition of potable Enterococci in water by :2000 waters membrane filtration.15 Bacteriological Detection of Salmonella spp in SOP PALM 0103 based on the condition of swimming water Microbiology of Drinking pools and spas Water 2006, Part 9.16 Bacteriological condition of environmental waters.11 Bacteriological condition of potable method: waters Detection and enumeration of SOP PALM 0104 based on the.15 Bacteriological sulphite reducing clostridia and Microbiology of Drinking condition of swimming Cl. perfringens in water by Water 2010, Part 6 pools and spas membrane filtration..16 Bacteriological condition of Chromogenic/ Fluorogenic MPN SOP PALM 0108 based on the environmental waters enumeration of coliform and E. Microbiology of Drinking coli using Colilert Quanti-Tray Water 2009 Part 4D. MPN. Edition 29, 09/07/ T Page 31 of 35

32 Public Analyst's Laboratory Biological Testing Laboratory Waters including effluents Bacteriological condition of potable waters Bacteriological condition of swimming pools and spas Bacteriological condition of environmental waters ELFA Detection of Salmonella spp using VIDAS SLM kit. Detection and Enumeration of thermotolerant Campylobacter spp. in water by the membrane filtration method method: SOP PALM 4011 based on AFNOR VIDAS Salmonella (VIDAS SLM) method BIO 12/1-04/94 (renewed 2010) Screening method. Cultural and confirmation aspects based on ISO 6579:2002 Amd SOP PALM 0062 based on ISO 17995: Microbiological tests Detection of Salmonella spp SOP PALM 0004 based on ISO for factory hygiene 6579:2002 Amd purposes Enumeration of SOP PALM 0009 based on ISO Surfaces Enterobacteriacae :2004 Stick swabs SOP PALM 0026 based on ISO Enumeration of β :2001 glucuronidase-postive E.coli by colony count at 44 C using TBX Enumeration of viable aerobic SOP PALM 0079 based on mesophilic flora using Tempo AFNOR TEMPO Validation TVC. BIO 12/15-09/05 (Renewed 2009) Edition 29, 09/07/ T Page 32 of 35

33 Public Analyst's Laboratory Biological Testing Laboratory 818 Microbiological tests for factory hygiene purposes Surfaces Stick swabs ELFA detection of Salmonella spp using VIDAS SLM kit SOP PALM 4001 based on AFNOR VIDAS Salmonella (VIDAS SLM) method BIO 12/1-04/94 (renewed 2010) Screening method. Cultural and confirmation aspects based on ISO 6579:2002 Amd Edition 29, 09/07/ T Page 33 of 35

34 Public Analyst's Laboratory Biological Testing Laboratory 818 Microbiological tests for factory hygiene purposes Surfaces Stick swabs Aerobic colony (Pour plate) at 30 C Aerobic colony count (spiral plate) at 30 C SOP PALM 0001 based on ISO 4833:2003 SOP PALM 0001(S) based on ISO4833:2003 Elfa detection of Campylobacter spp. Using VIDAS CAM kit SOP PALM 4003 based on AFNOR VIDAS Campylobacter (VIDAS CAM) method BIO 12/29-05/10 Screening method. Cultural and confirmation aspects based on ISO :2006 Detection of Campylobacter spp SOP PALM 0023 based on ISO :2006 Enumeration of Escherichia coli in food products using TEMPO EC(E coli) test SOP PALM 0005 based on AFNOR TEMPO EC validation BIO 12/13-02/05 (renewed 2009) Edition 29, 09/07/ T Page 34 of 35

35 Public Analyst's Laboratory Biological Testing Laboratory 810 Microbiological tests on cosmetics.12 Microbial count on Enumeration of aerobic SOP PALM 3000 based on ISO cosmetics mesophilic bacteria 21149:2006 Detection of Ps. Aeruginosa SOP PALM 3001 based on ISO 22717:2006 Enumeration of yeasts and SOP PALM 3003 based on ISO moulds 16212:2008 Edition 29, 09/07/ T Page 35 of 35